pycom10g23410

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004001
Physical Location & Seq
Reverse (-)
25572562 .. 25574894
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23410.1

Sequence Viewer

Length: 789 bp
ATGGCTTCCCCCAAAGAGAGAGAGAACTACGTCTACACTGCGAAGCTCGCCGAGCAAGCCGAGCGTTACGATGAAATGGTGGAGGCGATGACGAAAGTAGCGAAACTTGACGTGGAGCTCACTGTAGAGGAGCGGAATCTGCTTTCGGTCGGGTACAAGAACGTGATCGGAGCTCGCAGAGCTTCGTGGAGAATTCTGTCGTCGATTGAGCAGAAGGAGGAGGGGAAAGGGAACGATCAGAATGTGAGCCGTATCAAGGAGTACAGGAAAAAGGTCGAGTCCGAGCTCTCTAGCATTTGCAGCGACATCATGAGGGTTATTGATGAACATCTCATTCCGTCGTGCACTGCTTTTGAATCCACTGTGTTTTTCTACAAAATGAAAGGAGATTATTATCGCTACTTGGCGGAGTTTAAGACTGGTGATGACAGGAAAGAGGATGCCGATCAGTCAATGAAAGCTTATCAGGCAGCTTCTAGTAAAGCAGAAACTGATTTACCCCCCACACATCCCATCAGACTGGGTTTGGCCTTGAACTTTTCTGTCTTTTATTATGAAATCTTGAACTCTCCGGAAAGGGCATGTCACCTTGCAAAGCAAGCTTTTGATGAAGCTATCTCAGAATTGGACACTTTGAGTGAGGAATCATACAAAGACAGCACCTTGATCATGCAGCTCTTGAGGGACAACCTTACATTGTGGACCTCTGACATTCCAGAGGATGGAGGCGACGATATACAGAAGGGGGACGGCTCTGCTAAACCTGGAGGTGAAGATGCAGAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

263

Amino Acids

29.69

Weight (kDa)

4.75

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 13 - 235 1e-107 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 722
AccBSI CCGCTC 1 cut(s) 133
AccI GTMKAC 1 cut(s) 33
AccIII TCCGGA 1 cut(s) 571
AciI CCGC 2 cut(s) 133, 407
AcsI RAATTY 1 cut(s) 192
AfaI GTAC 2 cut(s) 155, 263
AfiI CCNNNNNNNGG 2 cut(s) 256, 722
AgsI TTSAA 3 cut(s) 356, 535, 565
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 1 cut(s) 763
Alw21I GWGCWC 4 cut(s) 120, 175, 288, 347
Alw44I GTGCAC 1 cut(s) 343
AlwNI CAGNNNCTG 1 cut(s) 491
Aor13HI TCCGGA 1 cut(s) 571
AoxI GGCC 1 cut(s) 528
ApaLI GTGCAC 1 cut(s) 343
ApeKI GCWGC 3 cut(s) 300, 470, 673
ApoI RAATTY 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 702
AsuHPI GGTGA 3 cut(s) 434, 578, 782
AvaII GGWCC 1 cut(s) 702
BaeGI GKGCMC 1 cut(s) 347
BanII GRGCYC 3 cut(s) 120, 175, 288
Bbv12I GWGCWC 4 cut(s) 120, 175, 288, 347
BbvI GCAGC 3 cut(s) 312, 482, 685
BccI CCATC 2 cut(s) 521, 716
BceAI ACGGC 2 cut(s) 234, 766
BciT130I CCWGG 1 cut(s) 765
BclI TGATCA 1 cut(s) 666
BfaI CTAG 2 cut(s) 291, 477
BfmI CTRYAG 1 cut(s) 123
BisI GCNGC 3 cut(s) 301, 471, 674
BlsI GCNGC 3 cut(s) 302, 472, 675
Bme1390I CCNGG 1 cut(s) 765
Bme18I GGWCC 1 cut(s) 702
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 702
BmrFI CCNGG 1 cut(s) 765
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 2 cut(s) 430, 766
BmuI ACTGGG 1 cut(s) 530
BpmI CTGGAG 1 cut(s) 786
BpuEI CTTGAG 1 cut(s) 700
BsaBI GATNNNNATC 3 cut(s) 327, 393, 444
BsaWI WCCGGW 1 cut(s) 571
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 722
Bse1I ACTGG 2 cut(s) 424, 525
Bse8I GATNNNNATC 3 cut(s) 327, 393, 444
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 1 cut(s) 765
BseGI GGATG 3 cut(s) 445, 508, 727
BseJI GATNNNNATC 3 cut(s) 327, 393, 444
BseLI CCNNNNNNNGG 2 cut(s) 256, 722
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 2 cut(s) 424, 525
BseRI GAGGAG 2 cut(s) 143, 233
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 3 cut(s) 312, 482, 685
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 1 cut(s) 530
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 4 cut(s) 120, 175, 288, 347
BsiSI CCGG 1 cut(s) 572
BslFI GGGAC 2 cut(s) 698, 761
BslI CCNNNNNNNGG 2 cut(s) 256, 722
BsmFI GGGAC 2 cut(s) 698, 761
BsnI GGCC 1 cut(s) 530
Bsp1286I GDGCHC 4 cut(s) 120, 175, 288, 347
Bsp13I TCCGGA 1 cut(s) 571
Bsp143I GATC 4 cut(s) 165, 235, 445, 666
BspACI CCGC 2 cut(s) 133, 407
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 1 cut(s) 632
BspEI TCCGGA 1 cut(s) 571
BspHI TCATGA 1 cut(s) 309
BsrBI CCGCTC 1 cut(s) 133
BsrI ACTGG 2 cut(s) 424, 525
BssMI GATC 4 cut(s) 165, 235, 445, 666
Bst2UI CCWGG 1 cut(s) 765
Bst4CI ACNGT 2 cut(s) 124, 364
BstC8I GCNNGC 4 cut(s) 48, 57, 175, 600
BstDEI CTNAG 1 cut(s) 619
BstF5I GGATG 3 cut(s) 445, 508, 727
BstKTI GATC 4 cut(s) 168, 238, 448, 669
BstMBI GATC 4 cut(s) 165, 235, 445, 666
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 9 cut(s) 47, 52, 56, 61, 139, 179, 300, 467, 599
BstNI CCWGG 1 cut(s) 765
BstNSI RCATGY 1 cut(s) 585
BstSCI CCNGG 1 cut(s) 763
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 347
BstV1I GCAGC 3 cut(s) 312, 482, 685
BstXI CCANNNNNNTGG 1 cut(s) 520
BsuRI GGCC 1 cut(s) 530
BtgZI GCGATG 1 cut(s) 101
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 3 cut(s) 445, 508, 727
BtsI GCAGTG 2 cut(s) 36, 345
BtsIMutI CAGTG 4 cut(s) 36, 120, 345, 360
Cac8I GCNNGC 4 cut(s) 48, 57, 175, 600
CaiI CAGNNNCTG 1 cut(s) 491
CciI TCATGA 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 702
Csp6I GTAC 2 cut(s) 154, 262
CviAII CATG 3 cut(s) 310, 582, 670
CviQI GTAC 2 cut(s) 154, 262
DdeI CTNAG 1 cut(s) 619
DpnI GATC 4 cut(s) 167, 237, 447, 668
DpnII GATC 4 cut(s) 165, 235, 445, 666
EciI GGCGGA 1 cut(s) 422
Ecl136II GAGCTC 3 cut(s) 118, 173, 286
Eco24I GRGCYC 3 cut(s) 120, 175, 288
Eco47I GGWCC 1 cut(s) 702
Eco53kI GAGCTC 3 cut(s) 118, 173, 286
EcoICRI GAGCTC 3 cut(s) 118, 173, 286
EcoRI GAATTC 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 763
EcoT38I GRGCYC 3 cut(s) 120, 175, 288
FaeI CATG 3 cut(s) 313, 585, 673
FaiI YATR 7 cut(s) 311, 555, 583, 649, 671, 737, 787
FaqI GGGAC 2 cut(s) 698, 761
FatI CATG 3 cut(s) 309, 581, 669
FbaI TGATCA 1 cut(s) 666
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 3 cut(s) 301, 471, 674
FokI GGATG 3 cut(s) 452, 495, 734
FriOI GRGCYC 3 cut(s) 120, 175, 288
Fsp4HI GCNGC 3 cut(s) 301, 471, 674
FspBI CTAG 2 cut(s) 291, 477
GluI GCNGC 3 cut(s) 301, 471, 674
GsuI CTGGAG 1 cut(s) 786
HaeIII GGCC 1 cut(s) 530
HapII CCGG 1 cut(s) 572
Hin1II CATG 3 cut(s) 313, 585, 673
HindIII AAGCTT 2 cut(s) 459, 600
HinfI GANTC 4 cut(s) 136, 278, 356, 644
HpaII CCGG 1 cut(s) 572
HphI GGTGA 3 cut(s) 434, 578, 782
Hpy166II GTNNAC 3 cut(s) 34, 345, 702
Hpy188I TCNGA 6 cut(s) 170, 240, 283, 518, 622, 709
Hpy188III TCNNGA 5 cut(s) 310, 562, 572, 679, 716
Hpy8I GTNNAC 3 cut(s) 34, 345, 702
Hpy99I CGWCG 3 cut(s) 205, 343, 734
HpyAV CCTTC 2 cut(s) 208, 736
HpyCH4III ACNGT 2 cut(s) 124, 364
HpyCH4IV ACGT 3 cut(s) 30, 111, 162
HpyCH4V TGCA 5 cut(s) 300, 345, 593, 673, 779
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 52, 56, 61, 139, 179, 300, 467, 599
HpyF3I CTNAG 1 cut(s) 619
HpySE526I ACGT 3 cut(s) 30, 111, 162
Hsp92II CATG 3 cut(s) 313, 585, 673
Kpn2I TCCGGA 1 cut(s) 571
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 4 cut(s) 165, 235, 445, 666
LmnI GCTCC 3 cut(s) 115, 130, 170
LpnPI CCDG 9 cut(s) 250, 405, 415, 452, 506, 585, 729, 750, 777
Lsp1109I GCAGC 3 cut(s) 312, 482, 685
LweI GCATC 2 cut(s) 430, 766
MaeI CTAG 2 cut(s) 291, 477
MaeII ACGT 3 cut(s) 30, 111, 162
MaeIII GTNAC 2 cut(s) 65, 584
MalI GATC 4 cut(s) 167, 237, 447, 668
MbiI CCGCTC 1 cut(s) 133
MboI GATC 4 cut(s) 165, 235, 445, 666
MboII GAAGA 1 cut(s) 785
MhlI GDGCHC 4 cut(s) 120, 175, 288, 347
MluCI AATT 2 cut(s) 192, 623
MlyI GAGTC 1 cut(s) 287
MroI TCCGGA 1 cut(s) 571
MseI TTAA 1 cut(s) 414
MspI CCGG 1 cut(s) 572
MspR9I CCNGG 1 cut(s) 765
MvaI CCWGG 1 cut(s) 765
MwoI GCNNNNNNNGC 9 cut(s) 47, 52, 56, 61, 139, 179, 300, 467, 599
NdeII GATC 4 cut(s) 165, 235, 445, 666
NlaIII CATG 3 cut(s) 313, 585, 673
NmeAIII GCCGAG 2 cut(s) 76, 85
NmuCI GTSAC 1 cut(s) 584
NspI RCATGY 1 cut(s) 585
PagI TCATGA 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PfeI GAWTC 3 cut(s) 136, 356, 644
PflMI CCANNNNNTGG 1 cut(s) 722
PkrI GCNGC 3 cut(s) 302, 472, 675
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Psp124BI GAGCTC 3 cut(s) 120, 175, 288
Psp6I CCWGG 1 cut(s) 763
PspGI CCWGG 1 cut(s) 763
PspPI GGNCC 1 cut(s) 702
PsrI GAACNNNNNNTAC 2 cut(s) 17, 49
PstNI CAGNNNCTG 1 cut(s) 491
RsaI GTAC 2 cut(s) 155, 263
RsaNI GTAC 2 cut(s) 154, 262
SacI GAGCTC 3 cut(s) 120, 175, 288
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 3 cut(s) 301, 471, 674
Sau3AI GATC 4 cut(s) 165, 235, 445, 666
Sau96I GGNCC 1 cut(s) 702
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 765
SduI GDGCHC 4 cut(s) 120, 175, 288, 347
SfaNI GCATC 2 cut(s) 430, 766
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 702
SmlI CTYRAG 1 cut(s) 679
SmoI CTYRAG 1 cut(s) 679
Sse9I AATT 2 cut(s) 192, 623
SsiI CCGC 2 cut(s) 133, 407
SspMI CTAG 2 cut(s) 291, 477
SstI GAGCTC 3 cut(s) 120, 175, 288
StyD4I CCNGG 1 cut(s) 763
TaaI ACNGT 2 cut(s) 124, 364
TaiI ACGT 3 cut(s) 33, 114, 165
TaqI TCGA 2 cut(s) 203, 276
TaqII GACCGA 1 cut(s) 136
TasI AATT 2 cut(s) 192, 623
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 3 cut(s) 136, 356, 644
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 4 cut(s) 43, 127, 352, 367
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 3 cut(s) 300, 470, 673
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 6 cut(s) 87, 339, 395, 470, 570, 624
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 4 cut(s) 43, 127, 352, 367
Van91I CCANNNNNTGG 1 cut(s) 722
VneI GTGCAC 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 702
XapI RAATTY 1 cut(s) 192
XceI RCATGY 1 cut(s) 585
XmiI GTMKAC 1 cut(s) 33
XspI CTAG 2 cut(s) 291, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.