Rh5DG079300

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004001
Physical Location & Seq
Forward (+)
6926465 .. 6930855
4391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG079300.1

Sequence Viewer

Length: 702 bp
ATGGCCGAGAGAGAGAACCACGTCTACACCGCCAAGCTCGCCGAGCAAGCCGAGCGCTACGACGAAATGGTGGAGGCGATGACGAAAGTGGCGAAGCTTGACGTGGAGCTGACTATCGAGGAGCGGAATCTGCTGTCTGTGGGGTACAAGAACGTGATCGGAGCACGGAGGGCTTCGTGGAGGATACTGTCGTCGATTGAGCAGAAGGAGGAGAGCAAAGGGAATGAGCAGAATGTGAGCCGTATCAAGGAGTACAGGAACAAGGTGGAGACTGAGCTCTCCAGCATTTGCAGTGACATCATGAGGGTTATTGATGAGCATCTCCTTCCGTCTTGCTCGGCTTTCGAGTCCACTGTGTTCTTCTACAAAATGAAAGGAGATTACTATCGCTACTTGGCTGAGTTTAAGACTGGTGATGACAGGAAGGAGGTTGCTGATCAGTCAATGAAGGCTTATCAGGCAGCTTCTAGCAAAGCAGAGACTGATTTACCTCCCACACATCCCATCAGGCTGGGATTGGCCTTGAACTTCTCTGTCTTTTACTATGAAATCTTGAATTCTCCTGAAAGGTATGCGTTTCATTGTGATCAATATTTGTGCTATAGATTTTTCTTTTTAACTAGTTTATGTAATGTAATGTTTTGTATGTTGACGGAAAGCAAAAGTAGAAGAAAAGAAATTTACTTGGTTGTACATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

233

Amino Acids

27.32

Weight (kDa)

6.03

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 10 - 190 1.1e-82 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 24
AciI CCGC 2 cut(s) 30, 124
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 556, 678
AfaI GTAC 3 cut(s) 146, 254, 693
AfeI AGCGCT 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 247
AgsI TTSAA 2 cut(s) 526, 556
AhlI ACTAGT 1 cut(s) 620
AjiI CACGTC 2 cut(s) 22, 103
AluBI AGCT 5 cut(s) 37, 97, 109, 277, 464
AluI AGCT 5 cut(s) 37, 97, 109, 277, 464
Alw21I GWGCWC 2 cut(s) 166, 279
Alw26I GTCTC 2 cut(s) 263, 473
AlwNI CAGNNNCTG 1 cut(s) 482
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 2 cut(s) 3, 519
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 2 cut(s) 556, 678
AspLEI GCGC 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 425
BaeI ACNNNNGTAYC 2 cut(s) 176, 209
BanII GRGCYC 1 cut(s) 279
Bbv12I GWGCWC 2 cut(s) 166, 279
BbvI GCAGC 1 cut(s) 473
BccI CCATC 1 cut(s) 512
BceAI ACGGC 1 cut(s) 225
BciVI GTATCC 1 cut(s) 177
BclI TGATCA 2 cut(s) 436, 586
BcoDI GTCTC 2 cut(s) 263, 473
BcuI ACTAGT 1 cut(s) 620
BfaI CTAG 2 cut(s) 468, 621
BfmI CTRYAG 1 cut(s) 601
BfoI RGCGCY 1 cut(s) 58
BfuI GTATCC 1 cut(s) 177
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
BmgBI CACGTC 2 cut(s) 22, 103
BmsI GCATC 1 cut(s) 328
BpmI CTGGAG 1 cut(s) 265
BsaBI GATNNNNATC 2 cut(s) 318, 384
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 415
Bse8I GATNNNNATC 2 cut(s) 318, 384
BseGI GGATG 1 cut(s) 499
BseJI GATNNNNATC 2 cut(s) 318, 384
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMII CTCAG 2 cut(s) 264, 390
BseNI ACTGG 1 cut(s) 415
BseRI GAGGAG 2 cut(s) 134, 224
BseXI GCAGC 1 cut(s) 473
BseYI CCCAGC 1 cut(s) 511
BshFI GGCC 2 cut(s) 5, 521
BsiHKAI GWGCWC 2 cut(s) 166, 279
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 2 cut(s) 263, 473
BsnI GGCC 2 cut(s) 5, 521
Bsp1286I GDGCHC 2 cut(s) 166, 279
Bsp1407I TGTACA 1 cut(s) 691
Bsp143I GATC 3 cut(s) 156, 436, 586
BspACI CCGC 2 cut(s) 30, 124
BspANI GGCC 2 cut(s) 5, 521
BspCNI CTCAG 2 cut(s) 265, 391
BspHI TCATGA 1 cut(s) 300
BsrBI CCGCTC 1 cut(s) 124
BsrGI TGTACA 1 cut(s) 691
BsrI ACTGG 1 cut(s) 415
BssMI GATC 3 cut(s) 156, 436, 586
Bst4CI ACNGT 2 cut(s) 189, 355
BstAUI TGTACA 1 cut(s) 691
BstC8I GCNNGC 2 cut(s) 39, 48
BstDEI CTNAG 2 cut(s) 273, 399
BstF5I GGATG 1 cut(s) 499
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 3 cut(s) 159, 439, 589
BstMAI GTCTC 2 cut(s) 263, 473
BstMBI GATC 3 cut(s) 156, 436, 586
BstMWI GCNNNNNNNGC 7 cut(s) 38, 43, 47, 52, 130, 170, 458
BstSFI CTRYAG 1 cut(s) 601
BstV1I GCAGC 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 511
BsuI GTATCC 1 cut(s) 177
BsuRI GGCC 2 cut(s) 5, 521
BtgZI GCGATG 1 cut(s) 92
BtrI CACGTC 2 cut(s) 22, 103
BtsCI GGATG 1 cut(s) 499
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 2 cut(s) 298, 351
Cac8I GCNNGC 2 cut(s) 39, 48
CaiI CAGNNNCTG 1 cut(s) 482
CciI TCATGA 1 cut(s) 300
CfoI GCGC 1 cut(s) 57
Csp6I GTAC 3 cut(s) 145, 253, 692
CviAII CATG 1 cut(s) 301
CviQI GTAC 3 cut(s) 145, 253, 692
DdeI CTNAG 2 cut(s) 273, 399
DpnI GATC 3 cut(s) 158, 438, 588
DpnII GATC 3 cut(s) 156, 436, 586
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 279
Eco47III AGCGCT 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 556
EcoT38I GRGCYC 1 cut(s) 279
FaeI CATG 1 cut(s) 304
FaiI YATR 6 cut(s) 302, 546, 573, 603, 628, 647
FatI CATG 1 cut(s) 300
FbaI TGATCA 2 cut(s) 436, 586
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 1 cut(s) 486
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 2 cut(s) 468, 621
GlaI GCGC 1 cut(s) 56
GluI GCNGC 1 cut(s) 462
GsaI CCCAGC 1 cut(s) 515
GsuI CTGGAG 1 cut(s) 265
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 2 cut(s) 5, 521
HhaI GCGC 1 cut(s) 57
Hin1II CATG 1 cut(s) 304
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 2 cut(s) 127, 347
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 3 cut(s) 25, 351, 651
Hpy188I TCNGA 1 cut(s) 161
Hpy188III TCNNGA 3 cut(s) 301, 553, 563
Hpy8I GTNNAC 3 cut(s) 25, 351, 651
Hpy99I CGWCG 2 cut(s) 65, 196
HpyAV CCTTC 4 cut(s) 199, 335, 418, 442
HpyCH4III ACNGT 2 cut(s) 189, 355
HpyCH4IV ACGT 3 cut(s) 21, 102, 153
HpyCH4V TGCA 1 cut(s) 291
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 43, 47, 52, 130, 170, 458
HpyF3I CTNAG 2 cut(s) 273, 399
HpySE526I ACGT 3 cut(s) 21, 102, 153
Hsp92II CATG 1 cut(s) 304
HspAI GCGC 1 cut(s) 55
Ksp22I TGATCA 2 cut(s) 436, 586
Kzo9I GATC 3 cut(s) 156, 436, 586
LmnI GCTCC 3 cut(s) 106, 121, 161
LpnPI CCDG 8 cut(s) 241, 295, 396, 406, 443, 493, 497, 576
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 1 cut(s) 328
MaeI CTAG 2 cut(s) 468, 621
MaeII ACGT 3 cut(s) 21, 102, 153
MaeIII GTNAC 1 cut(s) 293
MalI GATC 3 cut(s) 158, 438, 588
MbiI CCGCTC 1 cut(s) 124
MboI GATC 3 cut(s) 156, 436, 586
MboII GAAGA 2 cut(s) 352, 681
MhlI GDGCHC 2 cut(s) 166, 279
MluCI AATT 2 cut(s) 556, 678
MlyI GAGTC 1 cut(s) 356
MnlI CCTC 8 cut(s) 67, 112, 162, 174, 202, 297, 421, 501
MseI TTAA 2 cut(s) 405, 617
MwoI GCNNNNNNNGC 7 cut(s) 38, 43, 47, 52, 130, 170, 458
NdeII GATC 3 cut(s) 156, 436, 586
NlaIII CATG 1 cut(s) 304
NmeAIII GCCGAG 4 cut(s) 31, 67, 76, 317
NmuCI GTSAC 1 cut(s) 293
PagI TCATGA 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 1 cut(s) 463
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
Psp124BI GAGCTC 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 511
PsrI GAACNNNNNNTAC 2 cut(s) 8, 40
PstNI CAGNNNCTG 1 cut(s) 482
RsaI GTAC 3 cut(s) 146, 254, 693
RsaNI GTAC 3 cut(s) 145, 253, 692
SacI GAGCTC 1 cut(s) 279
SaqAI TTAA 2 cut(s) 405, 617
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 3 cut(s) 156, 436, 586
SchI GAGTC 1 cut(s) 356
SduI GDGCHC 2 cut(s) 166, 279
SfaNI GCATC 1 cut(s) 328
SfcI CTRYAG 1 cut(s) 601
SpeI ACTAGT 1 cut(s) 620
Sse9I AATT 2 cut(s) 556, 678
SsiI CCGC 2 cut(s) 30, 124
SspI AATATT 1 cut(s) 593
SspMI CTAG 2 cut(s) 468, 621
SstI GAGCTC 1 cut(s) 279
TaaI ACNGT 2 cut(s) 189, 355
TaiI ACGT 3 cut(s) 24, 105, 156
TaqI TCGA 3 cut(s) 117, 194, 345
TasI AATT 2 cut(s) 556, 678
TatI WGTACW 2 cut(s) 252, 691
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 405, 617
Tru9I TTAA 2 cut(s) 405, 617
TscAI CASTG 2 cut(s) 298, 358
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 4 cut(s) 386, 461, 561, 569
TspGWI ACGGA 3 cut(s) 181, 318, 668
TspRI CASTG 2 cut(s) 298, 358
XapI RAATTY 2 cut(s) 556, 678
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 2 cut(s) 468, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.