Prupe.3G121100_v2.0.a1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0004001
Physical Location & Seq
Forward (+)
10733503 .. 10739403
5901 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G121100.1

Sequence Viewer

Length: 795 bp
ATGGGTTTCGCTACAGAGCGTGAAAACTTCGTCTACCTTGCCAAGCTCTCTGAGCAGGCAGAGCGATACGATGAGATGGTGGATGCAATGAAGAAGGTTGCAAATCTTGATGTTGAATTGACAGTTGAAGAGAGAAACTTGCTCTCTGTTGGGTACAAGAATGTTGTTGGTTCTCGTCGAGCATCATGGAGGATCCTATCATCAATAGAACAGAAAGAGGAAGCAAAAGGAAATGAGAATCATGTGAAGCAACTCAAGGAGTATAGGCAGAAGGTTGAATCAGAGCTCTCGACCATTTGTGGTGATATCATGACAGTGATCGATGAGCATCTAATTCCTTCAGCTACAGTTGGTGAATCTACTGTTTTCTATTATAAGATGAAAGGAGATTATTATAGGTATCTTGCAGAATTCAAGAGCGGTGATGATAAAAAAGAGGCTGCTGAACAGTCGAAGAAAGCATATGAGAGTGCCACCATTGCTGCTGAAGGTGAGTTGCCAACTACACATCCCATCCGCTTGGGTCTGGCTTTGAATTTCTCAGTCTTCTATTATGAGATCATGAATTCTCCAGAAAGGGCTTGCCACCTTGCAAAGATGGCTTTTGATGAAGCTATTGCTGAGCTTGACTCGCTGAATGAGGAGTCATACAAAGATAGCACCTTAATCATGCAGCTTCTAAGGGACAACCTCACATTGTGGACTTCGGACCTTCCAGAGGATGGAGAAGATTCCCAGAAGGTGAATGGCACTGCCAAGGTTGGTGGGCTGGAAGATGCGGCCGTGACACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

265

Amino Acids

29.76

Weight (kDa)

4.76

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AccB7I CCANNNNNTGG 1 cut(s) 722
AccBSI CCGCTC 1 cut(s) 420
AccI GTMKAC 1 cut(s) 33
AciI CCGC 3 cut(s) 420, 517, 779
AclWI GGATC 2 cut(s) 187, 200
AcoI YGGCCR 1 cut(s) 780
AcsI RAATTY 3 cut(s) 410, 535, 565
AcuI CTGAAG 2 cut(s) 324, 507
AdeI CACNNNGTG 1 cut(s) 699
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 2 cut(s) 718, 722
AgsI TTSAA 5 cut(s) 116, 128, 278, 415, 535
AluBI AGCT 6 cut(s) 46, 286, 344, 614, 625, 676
AluI AGCT 6 cut(s) 46, 286, 344, 614, 625, 676
Alw21I GWGCWC 1 cut(s) 288
AlwI GGATC 2 cut(s) 187, 200
AoxI GGCC 1 cut(s) 780
ApeKI GCWGC 3 cut(s) 440, 482, 673
ApoI RAATTY 3 cut(s) 410, 535, 565
AspS9I GGNCC 1 cut(s) 709
AsuHPI GGTGA 5 cut(s) 314, 365, 434, 503, 754
AvaII GGWCC 1 cut(s) 709
BamHI GGATCC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 288
BbsI GAAGAC 1 cut(s) 538
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 3 cut(s) 427, 469, 685
BccI CCATC 4 cut(s) 70, 521, 592, 716
BceAI ACGGC 1 cut(s) 767
BfmI CTRYAG 2 cut(s) 12, 345
BisI GCNGC 4 cut(s) 441, 483, 674, 780
BlpI GCTNAGC 1 cut(s) 621
BlsI GCNGC 4 cut(s) 442, 484, 675, 781
Bme18I GGWCC 1 cut(s) 709
BmgT120I GGNCC 1 cut(s) 709
BmiI GGNNCC 1 cut(s) 194
BmsI GCATC 4 cut(s) 73, 191, 337, 766
BpiI GAAGAC 1 cut(s) 538
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 1 cut(s) 555
Bpu1102I GCTNAGC 1 cut(s) 621
BpuEI CTTGAG 1 cut(s) 239
Bsa29I ATCGAT 1 cut(s) 321
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 756
Bsc4I CCNNNNNNNGG 2 cut(s) 718, 722
Bse3DI GCAATG 2 cut(s) 93, 477
Bse8I GATNNNNATC 1 cut(s) 327
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 756
BseGI GGATG 4 cut(s) 88, 508, 513, 727
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 2 cut(s) 718, 722
BseMI GCAATG 2 cut(s) 93, 477
BseMII CTCAG 3 cut(s) 42, 555, 612
BseRI GAGGAG 1 cut(s) 656
BseX3I CGGCCG 1 cut(s) 780
BseXI GCAGC 3 cut(s) 427, 469, 685
Bsh1285I CGRYCG 1 cut(s) 783
BshFI GGCC 1 cut(s) 782
BshVI ATCGAT 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 783
BsiHKAI GWGCWC 1 cut(s) 288
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 2 cut(s) 718, 722
BsmFI GGGAC 1 cut(s) 698
BsnI GGCC 1 cut(s) 782
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 3 cut(s) 192, 318, 558
Bsp1720I GCTNAGC 1 cut(s) 621
BspACI CCGC 3 cut(s) 420, 517, 779
BspANI GGCC 1 cut(s) 782
BspCNI CTCAG 3 cut(s) 43, 554, 613
BspDI ATCGAT 1 cut(s) 321
BspHI TCATGA 2 cut(s) 309, 561
BspLI GGNNCC 1 cut(s) 194
BspPI GGATC 2 cut(s) 187, 200
BsrBI CCGCTC 1 cut(s) 420
BsrDI GCAATG 2 cut(s) 93, 477
BssECI CCNNGG 1 cut(s) 756
BssMI GATC 3 cut(s) 192, 318, 558
BssT1I CCWWGG 1 cut(s) 756
Bst4CI ACNGT 5 cut(s) 124, 316, 349, 364, 450
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 57, 583
BstDEI CTNAG 5 cut(s) 51, 541, 621, 680, 792
BstENI CCTNNNNNAGG 1 cut(s) 716
BstF5I GGATG 4 cut(s) 88, 508, 513, 727
BstKTI GATC 3 cut(s) 195, 321, 561
BstMBI GATC 3 cut(s) 192, 318, 558
BstMCI CGRYCG 1 cut(s) 783
BstMWI GCNNNNNNNGC 5 cut(s) 52, 61, 479, 599, 631
BstSFI CTRYAG 2 cut(s) 12, 345
BstV1I GCAGC 3 cut(s) 427, 469, 685
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 1 cut(s) 192
BstZI CGGCCG 1 cut(s) 780
Bsu15I ATCGAT 1 cut(s) 321
BsuRI GGCC 1 cut(s) 782
BsuTUI ATCGAT 1 cut(s) 321
BtsCI GGATG 4 cut(s) 88, 508, 513, 727
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 2 cut(s) 321, 750
Cac8I GCNNGC 2 cut(s) 57, 583
CciI TCATGA 2 cut(s) 309, 561
Cfr13I GGNCC 1 cut(s) 709
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 1 cut(s) 154
CspCI CAANNNNNGTGG 2 cut(s) 745, 780
CviAII CATG 5 cut(s) 186, 242, 310, 562, 670
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 5 cut(s) 51, 541, 621, 680, 792
DpnI GATC 3 cut(s) 194, 320, 560
DpnII GATC 3 cut(s) 192, 318, 558
DraIII CACNNNGTG 1 cut(s) 699
EaeI YGGCCR 1 cut(s) 780
EagI CGGCCG 1 cut(s) 780
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 286
EclXI CGGCCG 1 cut(s) 780
Eco130I CCWWGG 1 cut(s) 756
Eco24I GRGCYC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 709
Eco52I CGGCCG 1 cut(s) 780
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 2 cut(s) 324, 507
EcoICRI GAGCTC 1 cut(s) 286
EcoNI CCTNNNNNAGG 1 cut(s) 716
EcoRI GAATTC 2 cut(s) 410, 565
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 756
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 5 cut(s) 189, 245, 313, 565, 673
FaqI GGGAC 1 cut(s) 698
FatI CATG 5 cut(s) 185, 241, 309, 561, 669
FauNDI CATATG 1 cut(s) 463
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 4 cut(s) 441, 483, 674, 780
FokI GGATG 4 cut(s) 95, 495, 500, 734
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 4 cut(s) 441, 483, 674, 780
GluI GCNGC 4 cut(s) 441, 483, 674, 780
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 782
Hin1II CATG 5 cut(s) 189, 245, 313, 565, 673
HinfI GANTC 6 cut(s) 238, 278, 356, 629, 644, 731
HphI GGTGA 5 cut(s) 314, 365, 434, 503, 754
Hpy166II GTNNAC 2 cut(s) 34, 702
Hpy188I TCNGA 3 cut(s) 52, 283, 709
Hpy188III TCNNGA 7 cut(s) 107, 289, 310, 415, 562, 572, 716
Hpy8I GTNNAC 2 cut(s) 34, 702
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 6 cut(s) 88, 265, 348, 482, 722, 733
HpyCH4III ACNGT 5 cut(s) 124, 316, 349, 364, 450
HpyCH4V TGCA 5 cut(s) 86, 101, 407, 593, 673
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 61, 479, 599, 631
HpyF3I CTNAG 5 cut(s) 51, 541, 621, 680, 792
Hsp92II CATG 5 cut(s) 189, 245, 313, 565, 673
Kzo9I GATC 3 cut(s) 192, 318, 558
LpnPI CCDG 6 cut(s) 41, 512, 585, 729, 749, 755
Lsp1109I GCAGC 3 cut(s) 427, 469, 685
LweI GCATC 4 cut(s) 73, 191, 337, 766
MaeIII GTNAC 1 cut(s) 784
MalI GATC 3 cut(s) 194, 320, 560
MbiI CCGCTC 1 cut(s) 420
MboI GATC 3 cut(s) 192, 318, 558
MboII GAAGA 6 cut(s) 103, 140, 466, 538, 740, 785
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 5 cut(s) 116, 333, 410, 535, 565
MlyI GAGTC 2 cut(s) 623, 653
MnlI CCTC 6 cut(s) 183, 211, 430, 634, 701, 712
MseI TTAA 1 cut(s) 665
MslI CAYNNNNRTG 1 cut(s) 314
MwoI GCNNNNNNNGC 5 cut(s) 52, 61, 479, 599, 631
NdeI CATATG 1 cut(s) 463
NdeII GATC 3 cut(s) 192, 318, 558
NlaIII CATG 5 cut(s) 189, 245, 313, 565, 673
NlaIV GGNNCC 1 cut(s) 194
NmuCI GTSAC 1 cut(s) 784
PagI TCATGA 2 cut(s) 309, 561
PfeI GAWTC 4 cut(s) 238, 278, 356, 731
PflMI CCANNNNNTGG 1 cut(s) 722
PkrI GCNGC 4 cut(s) 442, 484, 675, 781
PleI GAGTC 2 cut(s) 623, 652
PpsI GAGTC 2 cut(s) 623, 652
PsiI TTATAA 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 194
PspPI GGNCC 1 cut(s) 709
PsuI RGATCY 1 cut(s) 192
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 314
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 665
SatI GCNGC 4 cut(s) 441, 483, 674, 780
Sau3AI GATC 3 cut(s) 192, 318, 558
Sau96I GGNCC 1 cut(s) 709
SchI GAGTC 2 cut(s) 623, 653
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 4 cut(s) 73, 191, 337, 766
SfcI CTRYAG 2 cut(s) 12, 345
SinI GGWCC 1 cut(s) 709
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 5 cut(s) 116, 333, 410, 535, 565
SsiI CCGC 3 cut(s) 420, 517, 779
SstI GAGCTC 1 cut(s) 288
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 5 cut(s) 124, 316, 349, 364, 450
TaqI TCGA 4 cut(s) 178, 290, 321, 452
TasI AATT 5 cut(s) 116, 333, 410, 535, 565
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 4 cut(s) 238, 278, 356, 731
Tru1I TTAA 1 cut(s) 665
Tru9I TTAA 1 cut(s) 665
TscAI CASTG 2 cut(s) 321, 757
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 3 cut(s) 440, 482, 673
Tsp45I GTSAC 1 cut(s) 784
TspDTI ATGAA 4 cut(s) 104, 395, 578, 624
TspRI CASTG 2 cut(s) 321, 757
Van91I CCANNNNNTGG 1 cut(s) 722
VpaK11BI GGWCC 1 cut(s) 709
XagI CCTNNNNNAGG 1 cut(s) 716
XapI RAATTY 3 cut(s) 410, 535, 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XmiI GTMKAC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.