FvH4_3g07810

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004001
Physical Location & Seq
Reverse (-)
4596479 .. 4600299
3821 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07810.t2

Sequence Viewer

Length: 777 bp
ATGGCTGAGAGAGACAACTACGTCTACACCGCCAAGCTCGCCGAGCAGGCCGAGCGCTACGACGAAATGGTGGAGGCGATGACGAAAGTGGCTAAGCTTGACGTGGAGCTGACTATTGAGGAGAGGAATCTGCTGTCTGTGGGGTACAAGAATGTGATTGGAGCTCGGAGGGCTTCGTGGAGGATACTGTCGTCGATTGAGCAGAAGGAGGAGGGCAAAGGGAATGATATCAATGTGAGCCGGATCAAGGAGTACAGGAACAAGGTGGAGGCTGAGCTTTCCAGCATTTGCAGTGACATCATGAGGGTTATTGATGAGCATCTCCTCCCGTCCTGCTCGGGATTTGAGTCCACTGTGTTCTTCTACAAAATGAAAGGCGATTACTATCGCTACTTGGCTGAGTTTAAGACTGGTGATGACAGGAAAGAGGTTGCTGATCAGTCAATGAAGGCTTATCAGGCAGCTTCAAGCAAGGCAGAGACTGATTTACCTCCCACTCATCCCATCAGGCTGGGATTGGCCTTGAACTTCTCTGTCTTTTACTATGAAATCTTAAACTCTCCTGAAAGAGCCTGTCACCTTGCAAAGCAAGCCTTTGATGAAGCTATCTCTGAGCTGGATACTTTGAGTGAGGAATCGTACAAGGACAGCACCTTAATCATGCAACTTCTGAGGGACAATCTTACACTGTGGACCTCTGACATTCCAGAGGATGGAGTTGAAGAAGGCCAGAAGGCAGACAGCTCTGCAAAAGCTGGAGGTGAAGATGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

259

Amino Acids

29.22

Weight (kDa)

4.73

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 1 - 210 2.4e-99 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 713
AccI GTMKAC 1 cut(s) 24
AciI CCGC 1 cut(s) 30
AclWI GGATC 1 cut(s) 251
AfaI GTAC 3 cut(s) 146, 254, 641
AfeI AGCGCT 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 247, 713
AgsI TTSAA 3 cut(s) 468, 526, 722
AjiI CACGTC 1 cut(s) 103
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 2 cut(s) 6, 473
AlwI GGATC 1 cut(s) 251
AlwNI CAGNNNCTG 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 337
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 3 cut(s) 48, 519, 727
ApeKI GCWGC 1 cut(s) 461
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 693
AsuHPI GGTGA 3 cut(s) 425, 569, 773
AvaI CYCGRG 1 cut(s) 337
AvaII GGWCC 1 cut(s) 693
BaeI ACNNNNGTAYC 2 cut(s) 176, 209
BanII GRGCYC 1 cut(s) 166
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 473
BccI CCATC 2 cut(s) 512, 707
BciVI GTATCC 2 cut(s) 177, 613
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 2 cut(s) 6, 473
BfoI RGCGCY 1 cut(s) 58
BfuI GTATCC 2 cut(s) 177, 613
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 1 cut(s) 462
BlpI GCTNAGC 2 cut(s) 93, 273
BlsI GCNGC 1 cut(s) 463
Bme18I GGWCC 1 cut(s) 693
BmeT110I CYCGRG 1 cut(s) 337
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 693
BmsI GCATC 2 cut(s) 328, 757
BpmI CTGGAG 1 cut(s) 777
Bpu1102I GCTNAGC 2 cut(s) 93, 273
BsaBI GATNNNNATC 2 cut(s) 318, 384
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 713
Bse1I ACTGG 1 cut(s) 415
Bse8I GATNNNNATC 2 cut(s) 318, 384
BseGI GGATG 2 cut(s) 499, 718
BseJI GATNNNNATC 2 cut(s) 318, 384
BseLI CCNNNNNNNGG 2 cut(s) 247, 713
BseMII CTCAG 4 cut(s) 264, 390, 603, 662
BseNI ACTGG 1 cut(s) 415
BseRI GAGGAG 3 cut(s) 134, 224, 314
BseXI GCAGC 1 cut(s) 473
BseYI CCCAGC 1 cut(s) 511
BshFI GGCC 3 cut(s) 50, 521, 729
BsiHKAI GWGCWC 1 cut(s) 166
BsiHKCI CYCGRG 1 cut(s) 337
BsiSI CCGG 1 cut(s) 241
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 2 cut(s) 247, 713
BsmAI GTCTC 2 cut(s) 6, 473
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 3 cut(s) 50, 521, 729
BsoBI CYCGRG 1 cut(s) 337
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 2 cut(s) 243, 436
Bsp1720I GCTNAGC 2 cut(s) 93, 273
BspACI CCGC 1 cut(s) 30
BspANI GGCC 3 cut(s) 50, 521, 729
BspCNI CTCAG 4 cut(s) 265, 391, 604, 663
BspHI TCATGA 1 cut(s) 300
BspPI GGATC 1 cut(s) 251
BsrI ACTGG 1 cut(s) 415
BssMI GATC 2 cut(s) 243, 436
Bst4CI ACNGT 3 cut(s) 189, 355, 690
BstC8I GCNNGC 3 cut(s) 39, 48, 591
BstDEI CTNAG 6 cut(s) 6, 93, 273, 399, 612, 671
BstF5I GGATG 2 cut(s) 499, 718
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 2 cut(s) 246, 439
BstMAI GTCTC 2 cut(s) 6, 473
BstMBI GATC 2 cut(s) 243, 436
BstMWI GCNNNNNNNGC 7 cut(s) 38, 43, 47, 52, 170, 458, 590
BstV1I GCAGC 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 511
BsuI GTATCC 2 cut(s) 177, 613
BsuRI GGCC 3 cut(s) 50, 521, 729
BtgZI GCGATG 1 cut(s) 92
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 2 cut(s) 499, 718
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 3 cut(s) 298, 351, 686
Cac8I GCNNGC 3 cut(s) 39, 48, 591
CaiI CAGNNNCTG 1 cut(s) 482
CciI TCATGA 1 cut(s) 300
CfoI GCGC 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 693
Csp6I GTAC 3 cut(s) 145, 253, 640
CviAII CATG 2 cut(s) 301, 661
CviQI GTAC 3 cut(s) 145, 253, 640
DdeI CTNAG 6 cut(s) 6, 93, 273, 399, 612, 671
DpnI GATC 2 cut(s) 245, 438
DpnII GATC 2 cut(s) 243, 436
DrdI GACNNNNNNGTC 1 cut(s) 20
DseDI GACNNNNNNGTC 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 166
Eco32I GATATC 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 693
Eco47III AGCGCT 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 337
EcoICRI GAGCTC 1 cut(s) 164
EcoRV GATATC 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 166
FaeI CATG 2 cut(s) 304, 664
FaiI YATR 3 cut(s) 302, 546, 662
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 689
FatI CATG 2 cut(s) 300, 660
FbaI TGATCA 1 cut(s) 436
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 2 cut(s) 486, 725
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 462
GlaI GCGC 1 cut(s) 56
GluI GCNGC 1 cut(s) 462
GsaI CCCAGC 1 cut(s) 515
GsuI CTGGAG 1 cut(s) 777
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 3 cut(s) 50, 521, 729
HapII CCGG 1 cut(s) 241
HhaI GCGC 1 cut(s) 57
Hin1II CATG 2 cut(s) 304, 664
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 3 cut(s) 127, 347, 635
HpaII CCGG 1 cut(s) 241
HphI GGTGA 3 cut(s) 425, 569, 773
Hpy166II GTNNAC 3 cut(s) 25, 351, 693
Hpy188I TCNGA 4 cut(s) 168, 613, 672, 700
Hpy188III TCNNGA 4 cut(s) 301, 339, 563, 707
Hpy8I GTNNAC 3 cut(s) 25, 351, 693
Hpy99I CGWCG 2 cut(s) 65, 196
HpyAV CCTTC 4 cut(s) 199, 442, 719, 727
HpyCH4III ACNGT 3 cut(s) 189, 355, 690
HpyCH4IV ACGT 2 cut(s) 21, 102
HpyCH4V TGCA 5 cut(s) 291, 584, 664, 749, 770
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 43, 47, 52, 170, 458, 590
HpyF3I CTNAG 6 cut(s) 6, 93, 273, 399, 612, 671
HpySE526I ACGT 2 cut(s) 21, 102
Hsp92II CATG 2 cut(s) 304, 664
HspAI GCGC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 2 cut(s) 243, 436
LmnI GCTCC 2 cut(s) 106, 161
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 2 cut(s) 328, 757
MaeII ACGT 2 cut(s) 21, 102
MaeIII GTNAC 2 cut(s) 293, 575
MalI GATC 2 cut(s) 245, 438
MboI GATC 2 cut(s) 243, 436
MboII GAAGA 3 cut(s) 352, 734, 776
MhlI GDGCHC 1 cut(s) 166
MlyI GAGTC 1 cut(s) 356
MseI TTAA 3 cut(s) 405, 554, 656
MspI CCGG 1 cut(s) 241
MwoI GCNNNNNNNGC 7 cut(s) 38, 43, 47, 52, 170, 458, 590
NdeII GATC 2 cut(s) 243, 436
NlaIII CATG 2 cut(s) 304, 664
NmeAIII GCCGAG 2 cut(s) 67, 76
NmuCI GTSAC 2 cut(s) 293, 575
PagI TCATGA 1 cut(s) 300
PfeI GAWTC 2 cut(s) 127, 635
PflMI CCANNNNNTGG 1 cut(s) 713
PkrI GCNGC 1 cut(s) 463
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
Psp124BI GAGCTC 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 511
PspPI GGNCC 1 cut(s) 693
PstNI CAGNNNCTG 1 cut(s) 482
RsaI GTAC 3 cut(s) 146, 254, 641
RsaNI GTAC 3 cut(s) 145, 253, 640
SacI GAGCTC 1 cut(s) 166
SaqAI TTAA 3 cut(s) 405, 554, 656
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 2 cut(s) 243, 436
Sau96I GGNCC 1 cut(s) 693
SchI GAGTC 1 cut(s) 356
SduI GDGCHC 1 cut(s) 166
SfaNI GCATC 2 cut(s) 328, 757
SinI GGWCC 1 cut(s) 693
SsiI CCGC 1 cut(s) 30
SstI GAGCTC 1 cut(s) 166
TaaI ACNGT 3 cut(s) 189, 355, 690
TaiI ACGT 2 cut(s) 24, 105
TaqI TCGA 1 cut(s) 194
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 2 cut(s) 127, 635
Tru1I TTAA 3 cut(s) 405, 554, 656
Tru9I TTAA 3 cut(s) 405, 554, 656
TscAI CASTG 3 cut(s) 298, 358, 693
TseFI GTSAC 2 cut(s) 293, 575
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 2 cut(s) 293, 575
TspDTI ATGAA 4 cut(s) 386, 461, 561, 615
TspRI CASTG 3 cut(s) 298, 358, 693
Van91I CCANNNNNTGG 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 693
XmiI GTMKAC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.