MD00G1176300.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004001
Physical Location & Seq
Forward (+)
40806633 .. 40810797
4165 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1176300.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGGTCTCGCTACTGAACGGGAGAACTTCGCTTACCTTGCCAAGCTCGCTGAGCAGGCAGAGCGATACGATGAGATGGTTGATGCAATGAAGAAGGTCGCAAATCTTGATGTTGAATTGACGGTTGAAGAGAGAAACTTGCTCTCTGTTGGGTACAAGAATGTTGTTGGTGCTCGTCGCGCATCATGGAGGATCCTATCATCAATTGAACAGAAAGAGGAAGCAAAAGGAAATGAGATTCATGTGAAGCAACTCAAGGAGTATAGACGGAAGGTTGAATCAGAGCTCTCAACCATTTGTAGTGATATCATGGCAGTGATCGATGAGCATCTCATTCCTTCAGCTACAGTTGGTGAATCAACTGTTTTCTATTATAAGATGAAAGGAGATTATTATAGGTATCTTGCAGAATTCAAGAGCGGTGACGATAAAAAAGAGGCTGCTGAACAGTCAAAGAAAGCATACGAGACTGCCACCACTACTGCTGAGGCCGAGTTGCCTTCCACGCATCCCATCCGTTTGGGTCTGGCTCTGAATTTCTCAGTCTTCTATTATGAGATCATGAATGCTCCAGAAAGTGCTTGCCACCTTGCAAAGATGGCTTTTGATGAAGCTATTGCTGAGCTTGACTCTTCGAATGAGGAGTCATACAAAGATAGCACCTTAATCATGCAGCTTTTAAGGGACAACCTCACATTGTGGACTTCTGACCATCCAGAAGATGGAGAGGATTCCCAAAAGGTGAATGGCAATGCCAAGGTTGGCGAGTTGGAAGAGGCAAACTCACAGATTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

266

Amino Acids

29.89

Weight (kDa)

4.78

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 13 - 235 1.7e-106 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AccB7I CCANNNNNTGG 1 cut(s) 722
AccBSI CCGCTC 1 cut(s) 420
AccII CGCG 1 cut(s) 180
AciI CCGC 1 cut(s) 420
AclWI GGATC 2 cut(s) 187, 200
AcsI RAATTY 2 cut(s) 410, 535
AcuI CTGAAG 1 cut(s) 324
AdeI CACNNNGTG 1 cut(s) 699
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 722
AgsI TTSAA 5 cut(s) 116, 128, 209, 278, 415
AluBI AGCT 6 cut(s) 46, 286, 344, 614, 625, 676
AluI AGCT 6 cut(s) 46, 286, 344, 614, 625, 676
Alw21I GWGCWC 2 cut(s) 175, 288
Alw26I GTCTC 2 cut(s) 11, 461
AlwI GGATC 2 cut(s) 187, 200
AoxI GGCC 1 cut(s) 489
ApeKI GCWGC 2 cut(s) 440, 673
ApoI RAATTY 2 cut(s) 410, 535
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
AspLEI GCGC 1 cut(s) 182
AsuHPI GGTGA 3 cut(s) 365, 434, 754
AsuII TTCGAA 1 cut(s) 635
BamHI GGATCC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 288
BbsI GAAGAC 1 cut(s) 538
Bbv12I GWGCWC 2 cut(s) 175, 288
BbvCI CCTCAGC 1 cut(s) 486
BbvI GCAGC 2 cut(s) 427, 685
BccI CCATC 5 cut(s) 70, 521, 592, 716, 720
BcoDI GTCTC 2 cut(s) 11, 461
BfmI CTRYAG 1 cut(s) 345
BisI GCNGC 2 cut(s) 441, 674
BlpI GCTNAGC 2 cut(s) 51, 621
BlsI GCNGC 2 cut(s) 442, 675
BmiI GGNNCC 1 cut(s) 194
BmsI GCATC 4 cut(s) 73, 191, 337, 517
BpiI GAAGAC 1 cut(s) 538
BplI GAGNNNNNCTC 3 cut(s) 614, 646, 767
BpmI CTGGAG 1 cut(s) 555
Bpu10I CCTNAGC 1 cut(s) 486
Bpu1102I GCTNAGC 2 cut(s) 51, 621
Bpu14I TTCGAA 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 239
Bsa29I ATCGAT 1 cut(s) 321
BsaBI GATNNNNATC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 756
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse3DI GCAATG 2 cut(s) 93, 757
Bse8I GATNNNNATC 1 cut(s) 327
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 756
BseGI GGATG 3 cut(s) 508, 513, 712
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMI GCAATG 2 cut(s) 93, 757
BseMII CTCAG 4 cut(s) 42, 477, 555, 612
BseRI GAGGAG 1 cut(s) 656
BseXI GCAGC 2 cut(s) 427, 685
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 1 cut(s) 491
BshVI ATCGAT 1 cut(s) 321
BsiHKAI GWGCWC 2 cut(s) 175, 288
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 1 cut(s) 722
BsmAI GTCTC 2 cut(s) 11, 461
BsmFI GGGAC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 571
BsnI GGCC 1 cut(s) 491
Bso31I GGTCTC 1 cut(s) 11
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 175, 288
Bsp143I GATC 3 cut(s) 192, 318, 558
Bsp1720I GCTNAGC 2 cut(s) 51, 621
BspACI CCGC 1 cut(s) 420
BspANI GGCC 1 cut(s) 491
BspCNI CTCAG 4 cut(s) 43, 478, 554, 613
BspDI ATCGAT 1 cut(s) 321
BspFNI CGCG 1 cut(s) 180
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 1 cut(s) 194
BspPI GGATC 2 cut(s) 187, 200
BspT104I TTCGAA 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 11
BsrBI CCGCTC 1 cut(s) 420
BsrDI GCAATG 2 cut(s) 93, 757
BssECI CCNNGG 1 cut(s) 756
BssMI GATC 3 cut(s) 192, 318, 558
BssT1I CCWWGG 1 cut(s) 756
Bst4CI ACNGT 4 cut(s) 124, 349, 364, 450
Bst6I CTCTTC 3 cut(s) 123, 637, 768
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 3 cut(s) 48, 57, 583
BstDEI CTNAG 5 cut(s) 51, 486, 541, 621, 795
BstF5I GGATG 3 cut(s) 508, 513, 712
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 3 cut(s) 195, 321, 561
BstMAI GTCTC 2 cut(s) 11, 461
BstMBI GATC 3 cut(s) 192, 318, 558
BstMWI GCNNNNNNNGC 8 cut(s) 38, 47, 52, 56, 61, 179, 505, 599
BstSFI CTRYAG 1 cut(s) 345
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 2 cut(s) 427, 685
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 1 cut(s) 192
Bsu15I ATCGAT 1 cut(s) 321
BsuRI GGCC 1 cut(s) 491
BsuTUI ATCGAT 1 cut(s) 321
BtsCI GGATG 3 cut(s) 508, 513, 712
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 1 cut(s) 321
Cac8I GCNNGC 3 cut(s) 48, 57, 583
CciI TCATGA 1 cut(s) 561
CfoI GCGC 1 cut(s) 182
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 1 cut(s) 154
CviAII CATG 5 cut(s) 186, 242, 310, 562, 670
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 5 cut(s) 51, 486, 541, 621, 795
DpnI GATC 3 cut(s) 194, 320, 560
DpnII GATC 3 cut(s) 192, 318, 558
DraIII CACNNNGTG 1 cut(s) 699
Eam1104I CTCTTC 3 cut(s) 123, 637, 768
EarI CTCTTC 3 cut(s) 123, 637, 768
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 756
Eco24I GRGCYC 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 307
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 324
EcoICRI GAGCTC 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 410
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 756
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 5 cut(s) 189, 245, 313, 565, 673
FaqI GGGAC 1 cut(s) 698
FatI CATG 5 cut(s) 185, 241, 309, 561, 669
Fnu4HI GCNGC 2 cut(s) 441, 674
FokI GGATG 3 cut(s) 495, 500, 699
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 2 cut(s) 441, 674
GlaI GCGC 1 cut(s) 181
GluI GCNGC 2 cut(s) 441, 674
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 491
HhaI GCGC 1 cut(s) 182
Hin1II CATG 5 cut(s) 189, 245, 313, 565, 673
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HinfI GANTC 6 cut(s) 238, 278, 356, 629, 644, 731
HphI GGTGA 3 cut(s) 365, 434, 754
Hpy166II GTNNAC 1 cut(s) 702
Hpy188I TCNGA 3 cut(s) 283, 534, 709
Hpy188III TCNNGA 5 cut(s) 107, 415, 562, 572, 716
Hpy8I GTNNAC 1 cut(s) 702
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 4 cut(s) 88, 265, 348, 510
HpyCH4III ACNGT 4 cut(s) 124, 349, 364, 450
HpyCH4V TGCA 4 cut(s) 86, 407, 593, 673
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 47, 52, 56, 61, 179, 505, 599
HpyF3I CTNAG 5 cut(s) 51, 486, 541, 621, 795
Hsp92II CATG 5 cut(s) 189, 245, 313, 565, 673
HspAI GCGC 1 cut(s) 180
Kzo9I GATC 3 cut(s) 192, 318, 558
LmnI GCTCC 1 cut(s) 574
LpnPI CCDG 4 cut(s) 41, 512, 585, 729
Lsp1109I GCAGC 2 cut(s) 427, 685
LweI GCATC 4 cut(s) 73, 191, 337, 517
MaeIII GTNAC 1 cut(s) 422
MalI GATC 3 cut(s) 194, 320, 560
MbiI CCGCTC 1 cut(s) 420
MboI GATC 3 cut(s) 192, 318, 558
MboII GAAGA 6 cut(s) 103, 140, 538, 624, 731, 785
MfeI CAATTG 1 cut(s) 204
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 2 cut(s) 175, 288
MluCI AATT 4 cut(s) 116, 204, 410, 535
MlyI GAGTC 2 cut(s) 623, 653
MmeI TCCRAC 1 cut(s) 750
MnlI CCTC 8 cut(s) 183, 211, 430, 481, 634, 701, 721, 769
MseI TTAA 2 cut(s) 665, 680
MslI CAYNNNNRTG 1 cut(s) 314
MunI CAATTG 1 cut(s) 204
Mva1269I GAATGC 1 cut(s) 571
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 8 cut(s) 38, 47, 52, 56, 61, 179, 505, 599
NdeII GATC 3 cut(s) 192, 318, 558
NlaIII CATG 5 cut(s) 189, 245, 313, 565, 673
NlaIV GGNNCC 1 cut(s) 194
NmeAIII GCCGAG 1 cut(s) 517
NmuCI GTSAC 1 cut(s) 422
NspV TTCGAA 1 cut(s) 635
PagI TCATGA 1 cut(s) 561
PctI GAATGC 1 cut(s) 571
PfeI GAWTC 4 cut(s) 238, 278, 356, 731
PflMI CCANNNNNTGG 1 cut(s) 722
PkrI GCNGC 2 cut(s) 442, 675
PleI GAGTC 2 cut(s) 623, 652
PpsI GAGTC 2 cut(s) 623, 652
PsiI TTATAA 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 194
PsrI GAACNNNNNNTAC 2 cut(s) 17, 49
PsuI RGATCY 1 cut(s) 192
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 314
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 665, 680
SatI GCNGC 2 cut(s) 441, 674
Sau3AI GATC 3 cut(s) 192, 318, 558
SchI GAGTC 2 cut(s) 623, 653
SduI GDGCHC 2 cut(s) 175, 288
SfaNI GCATC 4 cut(s) 73, 191, 337, 517
SfcI CTRYAG 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 4 cut(s) 116, 204, 410, 535
SsiI CCGC 1 cut(s) 420
SstI GAGCTC 1 cut(s) 288
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 4 cut(s) 124, 349, 364, 450
TaqI TCGA 2 cut(s) 321, 635
TasI AATT 4 cut(s) 116, 204, 410, 535
TfiI GAWTC 4 cut(s) 238, 278, 356, 731
Tru1I TTAA 2 cut(s) 665, 680
Tru9I TTAA 2 cut(s) 665, 680
TscAI CASTG 1 cut(s) 321
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 2 cut(s) 440, 673
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 5 cut(s) 104, 230, 395, 578, 624
TspGWI ACGGA 2 cut(s) 283, 506
TspRI CASTG 1 cut(s) 321
Van91I CCANNNNNTGG 1 cut(s) 722
XapI RAATTY 2 cut(s) 410, 535
XcmI CCANNNNNNNNNTGG 2 cut(s) 719, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.