Prupe.4G061000_v2.0.a1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0004001
Physical Location & Seq
Forward (+)
2903049 .. 2906544
3496 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G061000.3

Sequence Viewer

Length: 786 bp
ATGGCTTCCACCAAAGAGAGAGAGAACTACGTCTATACTGCTAAGCTCGCCGAACAAGCCGAGCGCTACGATGAAATGGTGGAGGCGATGGCCAAAGTAGCGAAGCTTGACGTGGAGCTCACCGTGGAGGAGCGAAATCTGCTTTCGGTTGGGTACAAGAACGTGATCGGAGCTCGTAGGGCTTCGTGGAGAATACTGTCATCGATCGAGCAGAAGGAGGAGGGGAAAGGGAACGATCAGAATGTGAGCCGAATCAAGGAATATAGGCACAAGGTCGAGTCTGAGCTCTCTAGCATTTGCAGTGACATCATGAGGGTTATCGATGAGCATCTCATTCCTTCCTGCACAGCTTTTGAGTCCACTGTGTTTTTCTACAAAATGAAGGGAGATTATTATCGCTATTTGGCAGAGTTTAAGACAGGTGATGACAGGAAAGAGGTTGCTGATCAGTCAATGAAAGCTTATCAGGCAGCTTCTAGTAAAGCAGAGGCAGACTTACCTCCTACACATCCCATCAGACTGGGTTTGGCCTTGAACTTCTCCGTCTTTTATTATGAAATCTTGAACTCTCCTGAAAGAGCCTGTCACCTGGCAAAGCAAGCTTTTGATGAAGCTATCTCAGAATTGGATACTTTGAGTGAGGAATCATACAAAGATAGCACATTGATCATGCAGCTCTTGAGGGACAACCTTACATTGTGGACCTCTGACATTCCAGAGGATGGAGCTGAGGAAGCCCAGAAGGTGGATGTCTCTGCTAAGCCTGGAGGTGAAGATGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

262

Amino Acids

29.61

Weight (kDa)

4.82

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 722, 745
AcoI YGGCCR 1 cut(s) 90
AfaI GTAC 1 cut(s) 155
AfeI AGCGCT 1 cut(s) 65
AfiI CCNNNNNNNGG 3 cut(s) 256, 722, 745
AgsI TTSAA 2 cut(s) 535, 565
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 2 cut(s) 588, 763
Alw21I GWGCWC 3 cut(s) 120, 175, 288
Alw26I GTCTC 1 cut(s) 757
Aor51HI AGCGCT 1 cut(s) 65
AoxI GGCC 2 cut(s) 90, 528
ApeKI GCWGC 2 cut(s) 470, 673
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 702
AsuHPI GGTGA 4 cut(s) 112, 434, 578, 782
AvaII GGWCC 1 cut(s) 702
BalI TGGCCA 1 cut(s) 92
BanII GRGCYC 3 cut(s) 120, 175, 288
Bbv12I GWGCWC 3 cut(s) 120, 175, 288
BbvCI CCTCAGC 1 cut(s) 729
BbvI GCAGC 2 cut(s) 482, 685
BccI CCATC 3 cut(s) 82, 521, 716
BciT130I CCWGG 2 cut(s) 590, 765
BciVI GTATCC 1 cut(s) 622
BclI TGATCA 2 cut(s) 445, 666
BcoDI GTCTC 1 cut(s) 757
BfaI CTAG 2 cut(s) 291, 477
BfoI RGCGCY 1 cut(s) 67
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 2 cut(s) 471, 674
BlpI GCTNAGC 2 cut(s) 42, 759
BlsI GCNGC 2 cut(s) 472, 675
Bme1390I CCNGG 2 cut(s) 590, 765
Bme18I GGWCC 1 cut(s) 702
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 702
BmrFI CCNGG 2 cut(s) 590, 765
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 2 cut(s) 337, 766
BmuI ACTGGG 1 cut(s) 530
BpmI CTGGAG 1 cut(s) 786
Bpu10I CCTNAGC 1 cut(s) 729
Bpu1102I GCTNAGC 2 cut(s) 42, 759
BpuEI CTTGAG 1 cut(s) 700
Bsa29I ATCGAT 2 cut(s) 203, 321
BsaBI GATNNNNATC 2 cut(s) 327, 393
BsaJI CCNNGG 1 cut(s) 123
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 722, 745
Bse1I ACTGG 1 cut(s) 525
Bse8I GATNNNNATC 2 cut(s) 327, 393
BseBI CCWGG 2 cut(s) 590, 765
BseCI ATCGAT 2 cut(s) 203, 321
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 3 cut(s) 508, 727, 754
BseJI GATNNNNATC 2 cut(s) 327, 393
BseLI CCNNNNNNNGG 3 cut(s) 256, 722, 745
BseMII CTCAG 3 cut(s) 273, 633, 720
BseNI ACTGG 1 cut(s) 525
BseRI GAGGAG 2 cut(s) 143, 233
BseXI GCAGC 2 cut(s) 482, 685
BsgI GTGCAG 1 cut(s) 328
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 2 cut(s) 92, 530
BshVI ATCGAT 2 cut(s) 203, 321
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 3 cut(s) 120, 175, 288
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 3 cut(s) 256, 722, 745
BsmAI GTCTC 1 cut(s) 757
BsmFI GGGAC 1 cut(s) 698
BsnI GGCC 2 cut(s) 92, 530
Bsp1286I GDGCHC 3 cut(s) 120, 175, 288
Bsp143I GATC 5 cut(s) 165, 204, 235, 445, 666
Bsp1720I GCTNAGC 2 cut(s) 42, 759
BspANI GGCC 2 cut(s) 92, 530
BspCNI CTCAG 3 cut(s) 274, 632, 721
BspDI ATCGAT 2 cut(s) 203, 321
BspHI TCATGA 1 cut(s) 309
BsrI ACTGG 1 cut(s) 525
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 5 cut(s) 165, 204, 235, 445, 666
Bst2UI CCWGG 2 cut(s) 590, 765
Bst4CI ACNGT 3 cut(s) 124, 198, 364
BstC8I GCNNGC 2 cut(s) 48, 600
BstDEI CTNAG 5 cut(s) 42, 282, 619, 729, 759
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 3 cut(s) 508, 727, 754
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 5 cut(s) 168, 207, 238, 448, 669
BstMAI GTCTC 1 cut(s) 757
BstMBI GATC 5 cut(s) 165, 204, 235, 445, 666
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 8 cut(s) 47, 56, 98, 139, 179, 467, 599, 734
BstNI CCWGG 2 cut(s) 590, 765
BstSCI CCNGG 2 cut(s) 588, 763
BstV1I GCAGC 2 cut(s) 482, 685
BstXI CCANNNNNNTGG 1 cut(s) 520
Bsu15I ATCGAT 2 cut(s) 203, 321
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 2 cut(s) 92, 530
BsuTUI ATCGAT 2 cut(s) 203, 321
BtgI CCRYGG 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 101
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 3 cut(s) 508, 727, 754
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 307, 360
Cac8I GCNNGC 2 cut(s) 48, 600
CciI TCATGA 1 cut(s) 309
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 702
ClaI ATCGAT 2 cut(s) 203, 321
Csp6I GTAC 1 cut(s) 154
CviAII CATG 2 cut(s) 310, 670
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 5 cut(s) 42, 282, 619, 729, 759
DpnI GATC 5 cut(s) 167, 206, 237, 447, 668
DpnII GATC 5 cut(s) 165, 204, 235, 445, 666
EaeI YGGCCR 1 cut(s) 90
Ecl136II GAGCTC 3 cut(s) 118, 173, 286
Eco24I GRGCYC 3 cut(s) 120, 175, 288
Eco47I GGWCC 1 cut(s) 702
Eco47III AGCGCT 1 cut(s) 65
Eco53kI GAGCTC 3 cut(s) 118, 173, 286
EcoICRI GAGCTC 3 cut(s) 118, 173, 286
EcoRII CCWGG 2 cut(s) 588, 763
EcoT38I GRGCYC 3 cut(s) 120, 175, 288
FaeI CATG 2 cut(s) 313, 673
FaiI YATR 6 cut(s) 36, 264, 311, 555, 649, 671
FaqI GGGAC 1 cut(s) 698
FatI CATG 2 cut(s) 309, 669
FbaI TGATCA 2 cut(s) 445, 666
Fnu4HI GCNGC 2 cut(s) 471, 674
FokI GGATG 3 cut(s) 495, 734, 761
FriOI GRGCYC 3 cut(s) 120, 175, 288
Fsp4HI GCNGC 2 cut(s) 471, 674
FspBI CTAG 2 cut(s) 291, 477
GlaI GCGC 1 cut(s) 65
GluI GCNGC 2 cut(s) 471, 674
GsuI CTGGAG 1 cut(s) 786
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 2 cut(s) 92, 530
HhaI GCGC 1 cut(s) 66
Hin1II CATG 2 cut(s) 313, 673
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HindIII AAGCTT 3 cut(s) 104, 459, 600
HinfI GANTC 4 cut(s) 252, 278, 356, 644
HphI GGTGA 4 cut(s) 112, 434, 578, 782
Hpy166II GTNNAC 2 cut(s) 360, 702
Hpy188I TCNGA 6 cut(s) 170, 240, 283, 518, 622, 709
Hpy188III TCNNGA 5 cut(s) 310, 562, 572, 679, 716
Hpy8I GTNNAC 2 cut(s) 360, 702
HpyAV CCTTC 4 cut(s) 208, 348, 376, 736
HpyCH4III ACNGT 3 cut(s) 124, 198, 364
HpyCH4IV ACGT 3 cut(s) 30, 111, 162
HpyCH4V TGCA 4 cut(s) 300, 345, 673, 779
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 56, 98, 139, 179, 467, 599, 734
HpyF3I CTNAG 5 cut(s) 42, 282, 619, 729, 759
HpySE526I ACGT 3 cut(s) 30, 111, 162
Hsp92II CATG 2 cut(s) 313, 673
HspAI GCGC 1 cut(s) 64
Ksp22I TGATCA 2 cut(s) 445, 666
Kzo9I GATC 5 cut(s) 165, 204, 235, 445, 666
LmnI GCTCC 4 cut(s) 115, 130, 170, 725
Lsp1109I GCAGC 2 cut(s) 482, 685
LweI GCATC 2 cut(s) 337, 766
MaeI CTAG 2 cut(s) 291, 477
MaeII ACGT 3 cut(s) 30, 111, 162
MaeIII GTNAC 2 cut(s) 302, 584
MalI GATC 5 cut(s) 167, 206, 237, 447, 668
MboI GATC 5 cut(s) 165, 204, 235, 445, 666
MboII GAAGA 1 cut(s) 785
MhlI GDGCHC 3 cut(s) 120, 175, 288
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 1 cut(s) 623
MluNI TGGCCA 1 cut(s) 92
MlyI GAGTC 2 cut(s) 287, 365
Mox20I TGGCCA 1 cut(s) 92
MscI TGGCCA 1 cut(s) 92
MseI TTAA 1 cut(s) 414
Msp20I TGGCCA 1 cut(s) 92
MspR9I CCNGG 2 cut(s) 590, 765
MvaI CCWGG 2 cut(s) 590, 765
MwoI GCNNNNNNNGC 8 cut(s) 47, 56, 98, 139, 179, 467, 599, 734
NdeII GATC 5 cut(s) 165, 204, 235, 445, 666
NlaIII CATG 2 cut(s) 313, 673
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 302, 584
PagI TCATGA 1 cut(s) 309
PfeI GAWTC 2 cut(s) 252, 644
PflMI CCANNNNNTGG 2 cut(s) 722, 745
PkrI GCNGC 2 cut(s) 472, 675
Ple19I CGATCG 1 cut(s) 207
PleI GAGTC 2 cut(s) 286, 364
PpsI GAGTC 2 cut(s) 286, 364
Psp124BI GAGCTC 3 cut(s) 120, 175, 288
Psp6I CCWGG 2 cut(s) 588, 763
PspGI CCWGG 2 cut(s) 588, 763
PspPI GGNCC 1 cut(s) 702
PvuI CGATCG 1 cut(s) 207
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SacI GAGCTC 3 cut(s) 120, 175, 288
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 2 cut(s) 471, 674
Sau3AI GATC 5 cut(s) 165, 204, 235, 445, 666
Sau96I GGNCC 1 cut(s) 702
SchI GAGTC 2 cut(s) 287, 365
ScrFI CCNGG 2 cut(s) 590, 765
SduI GDGCHC 3 cut(s) 120, 175, 288
SfaNI GCATC 2 cut(s) 337, 766
SinI GGWCC 1 cut(s) 702
SmlI CTYRAG 1 cut(s) 679
SmoI CTYRAG 1 cut(s) 679
Sse9I AATT 1 cut(s) 623
SspMI CTAG 2 cut(s) 291, 477
SstI GAGCTC 3 cut(s) 120, 175, 288
StyD4I CCNGG 2 cut(s) 588, 763
TaaI ACNGT 3 cut(s) 124, 198, 364
TaiI ACGT 3 cut(s) 33, 114, 165
TaqI TCGA 4 cut(s) 203, 207, 276, 321
TasI AATT 1 cut(s) 623
TfiI GAWTC 2 cut(s) 252, 644
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 2 cut(s) 307, 367
TseFI GTSAC 2 cut(s) 302, 584
TseI GCWGC 2 cut(s) 470, 673
Tsp45I GTSAC 2 cut(s) 302, 584
TspDTI ATGAA 5 cut(s) 87, 395, 470, 570, 624
TspGWI ACGGA 1 cut(s) 532
TspRI CASTG 2 cut(s) 307, 367
Van91I CCANNNNNTGG 2 cut(s) 722, 745
VpaK11BI GGWCC 1 cut(s) 702
XspI CTAG 2 cut(s) 291, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.