Rw0G022290

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01032
OG0004001
Physical Location & Seq
Reverse (-)
112944 .. 116409
3466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022290.1

Sequence Viewer

Length: 774 bp
ATGGCCGAGAGAGAGAACCACGTCTACACCGCCAAGCTCGCCGAGCAAGCCGAGCGCTACGACGAAATGGTGGAGGCGATGACGAAAGTGGCGAAGCTTGACGTGGAGCTGACTATCGAGGAGCGGAATCTGCTGTCTGTGGGGTACAAGAACGTGATCGGAGCACGGAGGGCTTCGTGGAGGATTCTGTCGTCGATTGAGCAGAAGGAGGAGAGCAAAGGGAATGAGCAGAATGTGAGCCGTATCAAGGAGTACAGGAACAAGGTGGAGACTGAGCTCTCCAGCATTTGCAGTGACATCATGAGGGTTATTGATGAGCATCTCCTTCCGTCTTGCTCGGCTTTCGAGTCCACTGTGTTCTTCTACAAAATGAAAGGAGATTACTATCGCTACTTGGCTGAGTTTAAGACTGGTGATGACAGGAAGGAGGTTGCTGATCAGTCAATGAAGGCTTATCAGGCAGCTTCTAGCAAAGCAGAGACTGATTTACCTCCCACACATCCCATCAGGCTGGGATTGGCCTTGAACTTCTCTGTCTTTTACTATGAAATCTTGAATTCTCCTGAAAGAGCCTGTCATCTTGCAAAGCAAGCCTTTGATGAAGCTATCTCCGAGCTGGATACTTTGAGTGAGGAGTCATACAAGGACAGCACCTTAATCATGCAACTCTTGAGGGACAACCTCACATTGTGGACCTCTGACATTCCGGAGGATGGAGTTGAAGAAGGGCAGAAGGCGGACAGCTCTGCAAAAACTGGAGGTGAAGATGCAGAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

258

Amino Acids

29.34

Weight (kDa)

4.78

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 10 - 232 5.7e-108 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 24
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 3 cut(s) 30, 124, 737
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 556
AdeI CACNNNGTG 1 cut(s) 690
AfaI GTAC 2 cut(s) 146, 254
AfeI AGCGCT 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 247, 713
AgsI TTSAA 3 cut(s) 526, 556, 722
AjiI CACGTC 2 cut(s) 22, 103
AluBI AGCT 8 cut(s) 37, 97, 109, 277, 464, 605, 616, 744
AluI AGCT 8 cut(s) 37, 97, 109, 277, 464, 605, 616, 744
Alw21I GWGCWC 2 cut(s) 166, 279
Alw26I GTCTC 2 cut(s) 263, 473
AlwNI CAGNNNCTG 1 cut(s) 482
Aor13HI TCCGGA 1 cut(s) 706
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 2 cut(s) 3, 519
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 1 cut(s) 556
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 693
AsuHPI GGTGA 2 cut(s) 425, 773
AvaII GGWCC 1 cut(s) 693
BanII GRGCYC 1 cut(s) 279
Bbv12I GWGCWC 2 cut(s) 166, 279
BbvI GCAGC 1 cut(s) 473
BccI CCATC 2 cut(s) 512, 707
BceAI ACGGC 1 cut(s) 225
BciVI GTATCC 1 cut(s) 613
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 2 cut(s) 263, 473
BfaI CTAG 1 cut(s) 468
BfoI RGCGCY 1 cut(s) 58
BfuI GTATCC 1 cut(s) 613
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
Bme18I GGWCC 1 cut(s) 693
BmgBI CACGTC 2 cut(s) 22, 103
BmgT120I GGNCC 1 cut(s) 693
BmsI GCATC 2 cut(s) 328, 757
BpmI CTGGAG 1 cut(s) 265
BpuEI CTTGAG 1 cut(s) 691
BsaBI GATNNNNATC 2 cut(s) 318, 384
BsaWI WCCGGW 1 cut(s) 706
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 713
Bse1I ACTGG 2 cut(s) 415, 760
Bse8I GATNNNNATC 2 cut(s) 318, 384
BseAI TCCGGA 1 cut(s) 706
BseGI GGATG 2 cut(s) 499, 718
BseJI GATNNNNATC 2 cut(s) 318, 384
BseLI CCNNNNNNNGG 2 cut(s) 247, 713
BseMII CTCAG 2 cut(s) 264, 390
BseNI ACTGG 2 cut(s) 415, 760
BseRI GAGGAG 3 cut(s) 134, 224, 647
BseXI GCAGC 1 cut(s) 473
BseYI CCCAGC 1 cut(s) 511
BshFI GGCC 2 cut(s) 5, 521
BsiHKAI GWGCWC 2 cut(s) 166, 279
BsiSI CCGG 1 cut(s) 707
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 2 cut(s) 247, 713
BsmAI GTCTC 2 cut(s) 263, 473
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 2 cut(s) 5, 521
Bsp1286I GDGCHC 2 cut(s) 166, 279
Bsp13I TCCGGA 1 cut(s) 706
Bsp143I GATC 2 cut(s) 156, 436
BspACI CCGC 3 cut(s) 30, 124, 737
BspANI GGCC 2 cut(s) 5, 521
BspCNI CTCAG 2 cut(s) 265, 391
BspEI TCCGGA 1 cut(s) 706
BspHI TCATGA 1 cut(s) 300
BsrBI CCGCTC 1 cut(s) 124
BsrI ACTGG 2 cut(s) 415, 760
BssMI GATC 2 cut(s) 156, 436
Bst4CI ACNGT 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 39, 48, 591
BstDEI CTNAG 2 cut(s) 273, 399
BstF5I GGATG 2 cut(s) 499, 718
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 2 cut(s) 159, 439
BstMAI GTCTC 2 cut(s) 263, 473
BstMBI GATC 2 cut(s) 156, 436
BstMWI GCNNNNNNNGC 8 cut(s) 38, 43, 47, 52, 130, 170, 458, 590
BstV1I GCAGC 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 511
BsuI GTATCC 1 cut(s) 613
BsuRI GGCC 2 cut(s) 5, 521
BtgZI GCGATG 1 cut(s) 92
BtrI CACGTC 2 cut(s) 22, 103
BtsCI GGATG 2 cut(s) 499, 718
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 2 cut(s) 298, 351
Cac8I GCNNGC 3 cut(s) 39, 48, 591
CaiI CAGNNNCTG 1 cut(s) 482
CciI TCATGA 1 cut(s) 300
CfoI GCGC 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 693
Csp6I GTAC 2 cut(s) 145, 253
CviAII CATG 2 cut(s) 301, 661
CviQI GTAC 2 cut(s) 145, 253
DdeI CTNAG 2 cut(s) 273, 399
DpnI GATC 2 cut(s) 158, 438
DpnII GATC 2 cut(s) 156, 436
DraIII CACNNNGTG 1 cut(s) 690
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 752
Ecl136II GAGCTC 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 693
Eco47III AGCGCT 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 556
EcoT38I GRGCYC 1 cut(s) 279
FaeI CATG 2 cut(s) 304, 664
FaiI YATR 4 cut(s) 302, 546, 640, 662
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 689
FatI CATG 2 cut(s) 300, 660
FbaI TGATCA 1 cut(s) 436
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 2 cut(s) 486, 725
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 1 cut(s) 468
GlaI GCGC 1 cut(s) 56
GluI GCNGC 1 cut(s) 462
GsaI CCCAGC 1 cut(s) 515
GsuI CTGGAG 1 cut(s) 265
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 2 cut(s) 5, 521
HapII CCGG 1 cut(s) 707
HhaI GCGC 1 cut(s) 57
Hin1II CATG 2 cut(s) 304, 664
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 4 cut(s) 127, 184, 347, 635
HpaII CCGG 1 cut(s) 707
HphI GGTGA 2 cut(s) 425, 773
Hpy166II GTNNAC 3 cut(s) 25, 351, 693
Hpy188I TCNGA 3 cut(s) 161, 613, 700
Hpy188III TCNNGA 5 cut(s) 301, 553, 563, 670, 707
Hpy8I GTNNAC 3 cut(s) 25, 351, 693
Hpy99I CGWCG 2 cut(s) 65, 196
HpyAV CCTTC 6 cut(s) 199, 335, 418, 442, 719, 727
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 3 cut(s) 21, 102, 153
HpyCH4V TGCA 5 cut(s) 291, 584, 664, 749, 770
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 43, 47, 52, 130, 170, 458, 590
HpyF3I CTNAG 2 cut(s) 273, 399
HpySE526I ACGT 3 cut(s) 21, 102, 153
Hsp92II CATG 2 cut(s) 304, 664
HspAI GCGC 1 cut(s) 55
Kpn2I TCCGGA 1 cut(s) 706
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 2 cut(s) 156, 436
LmnI GCTCC 3 cut(s) 106, 121, 161
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 2 cut(s) 328, 757
MaeI CTAG 1 cut(s) 468
MaeII ACGT 3 cut(s) 21, 102, 153
MaeIII GTNAC 1 cut(s) 293
MalI GATC 2 cut(s) 158, 438
MbiI CCGCTC 1 cut(s) 124
MboI GATC 2 cut(s) 156, 436
MboII GAAGA 2 cut(s) 352, 734
MhlI GDGCHC 2 cut(s) 166, 279
MluCI AATT 1 cut(s) 556
MlyI GAGTC 2 cut(s) 356, 644
MroI TCCGGA 1 cut(s) 706
MseI TTAA 2 cut(s) 405, 656
MspI CCGG 1 cut(s) 707
MwoI GCNNNNNNNGC 8 cut(s) 38, 43, 47, 52, 130, 170, 458, 590
NdeII GATC 2 cut(s) 156, 436
NlaIII CATG 2 cut(s) 304, 664
NmeAIII GCCGAG 4 cut(s) 31, 67, 76, 317
NmuCI GTSAC 1 cut(s) 293
PagI TCATGA 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PfeI GAWTC 2 cut(s) 127, 184
PkrI GCNGC 1 cut(s) 463
PleI GAGTC 2 cut(s) 355, 643
PpsI GAGTC 2 cut(s) 355, 643
Psp124BI GAGCTC 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 511
PspPI GGNCC 1 cut(s) 693
PsrI GAACNNNNNNTAC 2 cut(s) 8, 40
PstNI CAGNNNCTG 1 cut(s) 482
RsaI GTAC 2 cut(s) 146, 254
RsaNI GTAC 2 cut(s) 145, 253
SacI GAGCTC 1 cut(s) 279
SaqAI TTAA 2 cut(s) 405, 656
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 2 cut(s) 156, 436
Sau96I GGNCC 1 cut(s) 693
SchI GAGTC 2 cut(s) 356, 644
SduI GDGCHC 2 cut(s) 166, 279
SfaNI GCATC 2 cut(s) 328, 757
SinI GGWCC 1 cut(s) 693
SmlI CTYRAG 1 cut(s) 670
SmoI CTYRAG 1 cut(s) 670
Sse9I AATT 1 cut(s) 556
SsiI CCGC 3 cut(s) 30, 124, 737
SspMI CTAG 1 cut(s) 468
SstI GAGCTC 1 cut(s) 279
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 3 cut(s) 24, 105, 156
TaqI TCGA 3 cut(s) 117, 194, 345
TasI AATT 1 cut(s) 556
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 2 cut(s) 127, 184
Tru1I TTAA 2 cut(s) 405, 656
Tru9I TTAA 2 cut(s) 405, 656
TscAI CASTG 2 cut(s) 298, 358
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 4 cut(s) 386, 461, 561, 615
TspGWI ACGGA 2 cut(s) 181, 318
TspRI CASTG 2 cut(s) 298, 358
VpaK11BI GGWCC 1 cut(s) 693
XapI RAATTY 1 cut(s) 556
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.