MD10G1280200.v1.1

Olee1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009996
Physical Location & Seq
Reverse (-)
37118140 .. 37119013
874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1280200.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGCCAATTCTAATAGTATCGTTCTTGTCTCTGCCCTTTGCTTCTTGTCGCTCATCGGCGTCGCCTACTCCACCGAGACCCTCAATGTCCACGGCATTGTTTATTGCGATAATTGCCGCACGCAGTTCTTTACCAGAATCAGTGAACCCCTCACAGGTGCAAAGGTGAGATTGGAATGCAGAGAGAGTGAAGGGGGAAAAGTAACTCTAACCAAAGAGGTAGACACCGAGGAATTAGGAACTTACTCCATTCCGGTGGAAGGAGACCATGAAGAGGAGGTTTGCGAGGTGATCTTGGTGAAGAGTCCCAAGGAGGACTGCAGCGAGATCAGCAACGAGCTTCATGCGAAGCTCAGCGCGAGGATCAGCCTCACGAACCACAATGGCATCACCGGGCCTCATCGCATGGCGAACCCTCTCGGTTTCATGAAGAAGGAGGTTGATCCTAGGTGCGCTGAGGTCCTCAAGGAGCTCGGACTCACCCCCGATGGCGAATTTATTGACGGCGCTCCTGATGCCGCTCCTGATGGCGCTGCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

181

Amino Acids

19.46

Weight (kDa)

4.73

Isoelectric Point (pI)

22.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 30 - 114 1.8e-19 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29140 AT4G18596 AT4G18596 AT5G45880
fragaria_vesca FvH4_3g07791
malus_domestica MD05G1301300.v1.1 MD10G1280200.v1.1
prunus_persica Prupe.4G061100_v2.0.a1
pyrus_communis pycom05g27910
rosa_chinensis RchiOBHm_Chr5g0010771
rosa_laevigata RLG00000031778
rosa_multiflora Rmu_sc0000109.1_g000021
rosa_roxburghii Rroxscaffold_1G00065610
rosa_rugosa Rorug04G0452100
rosa_samantha Rh5AG084000 Rh5AG084400 Rh5BG079200 Rh5CG092000 Rh5DG079400
rosa_wichuraiana Rw0G000580 Rw0G022280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 521
AccI GTMKAC 1 cut(s) 222
AccII CGCG 1 cut(s) 359
AciI CCGC 2 cut(s) 118, 519
AclWI GGATC 2 cut(s) 371, 437
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 60
AfiI CCNNNNNNNGG 3 cut(s) 155, 260, 274
AluBI AGCT 3 cut(s) 340, 352, 472
AluI AGCT 3 cut(s) 340, 352, 472
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 3 cut(s) 34, 71, 258
AlwI GGATC 2 cut(s) 371, 437
AoxI GGCC 2 cut(s) 3, 395
ApeKI GCWGC 2 cut(s) 321, 533
ApoI RAATTY 1 cut(s) 494
AspA2I CCTAGG 1 cut(s) 446
AspLEI GCGC 4 cut(s) 359, 455, 509, 533
AspS9I GGNCC 2 cut(s) 395, 460
AsuC2I CCSGG 1 cut(s) 394
AsuHPI GGTGA 5 cut(s) 178, 301, 310, 382, 472
AvaII GGWCC 1 cut(s) 460
AvrII CCTAGG 1 cut(s) 446
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 2 cut(s) 333, 520
BccI CCATC 2 cut(s) 482, 521
BceAI ACGGC 2 cut(s) 109, 520
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BcnI CCSGG 1 cut(s) 394
BcoDI GTCTC 3 cut(s) 34, 71, 258
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 2 cut(s) 510, 534
BisI GCNGC 4 cut(s) 118, 322, 519, 534
BlnI CCTAGG 1 cut(s) 446
BlpI GCTNAGC 1 cut(s) 353
BlsI GCNGC 4 cut(s) 119, 323, 520, 535
Bme1390I CCNGG 1 cut(s) 394
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 2 cut(s) 395, 460
BmrFI CCNGG 1 cut(s) 394
BmsI GCATC 2 cut(s) 396, 505
Bpu10I CCTNAGC 1 cut(s) 456
Bpu1102I GCTNAGC 1 cut(s) 353
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 2 cut(s) 71, 258
BsaJI CCNNGG 4 cut(s) 91, 228, 309, 446
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 260, 274
BseDI CCNNGG 4 cut(s) 91, 228, 309, 446
BseLI CCNNNNNNNGG 3 cut(s) 155, 260, 274
BseMII CTCAG 2 cut(s) 367, 447
BseRI GAGGAG 1 cut(s) 290
BseXI GCAGC 2 cut(s) 333, 520
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 2 cut(s) 5, 397
BsiHKAI GWGCWC 1 cut(s) 474
BsiSI CCGG 2 cut(s) 254, 393
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 3 cut(s) 155, 260, 274
BsmAI GTCTC 3 cut(s) 34, 71, 258
BsmFI GGGAC 1 cut(s) 291
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 2 cut(s) 5, 397
Bso31I GGTCTC 2 cut(s) 71, 258
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 4 cut(s) 291, 327, 363, 442
Bsp1720I GCTNAGC 1 cut(s) 353
BspACI CCGC 2 cut(s) 118, 519
BspANI GGCC 2 cut(s) 5, 397
BspCNI CTCAG 2 cut(s) 366, 448
BspFNI CGCG 1 cut(s) 359
BspHI TCATGA 1 cut(s) 426
BspMAI CTGCAG 1 cut(s) 323
BspPI GGATC 2 cut(s) 371, 437
BspTNI GGTCTC 2 cut(s) 71, 258
BsrBI CCGCTC 1 cut(s) 521
BssECI CCNNGG 4 cut(s) 91, 228, 309, 446
BssMI GATC 4 cut(s) 291, 327, 363, 442
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 2 cut(s) 309, 446
Bst6I CTCTTC 2 cut(s) 267, 296
BstACI GRCGYC 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 2 cut(s) 353, 456
BstDSI CCRYGG 1 cut(s) 91
BstFNI CGCG 1 cut(s) 359
BstH2I RGCGCY 2 cut(s) 510, 534
BstHHI GCGC 4 cut(s) 359, 455, 509, 533
BstKTI GATC 4 cut(s) 294, 330, 366, 445
BstMAI GTCTC 3 cut(s) 34, 71, 258
BstMBI GATC 4 cut(s) 291, 327, 363, 442
BstMWI GCNNNNNNNGC 3 cut(s) 114, 330, 515
BstSCI CCNGG 1 cut(s) 392
BstSFI CTRYAG 1 cut(s) 319
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 2 cut(s) 333, 520
BstXI CCANNNNNNTGG 1 cut(s) 256
BsuRI GGCC 2 cut(s) 5, 397
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 386
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 1 cut(s) 122
CciI TCATGA 1 cut(s) 426
CfoI GCGC 4 cut(s) 359, 455, 509, 533
Cfr13I GGNCC 2 cut(s) 395, 460
CseI GACGC 1 cut(s) 49
CviAII CATG 4 cut(s) 269, 344, 406, 427
CviJI RGCY 6 cut(s) 5, 340, 352, 369, 397, 472
CviKI_1 RGCY 6 cut(s) 5, 340, 352, 369, 397, 472
DdeI CTNAG 2 cut(s) 353, 456
DpnI GATC 4 cut(s) 293, 329, 365, 444
DpnII GATC 4 cut(s) 291, 327, 363, 442
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 267, 296
EarI CTCTTC 2 cut(s) 267, 296
Ecl136II GAGCTC 1 cut(s) 472
Eco130I CCWWGG 2 cut(s) 309, 446
Eco24I GRGCYC 1 cut(s) 474
Eco31I GGTCTC 2 cut(s) 71, 258
Eco47I GGWCC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 472
EcoO109I RGGNCCY 1 cut(s) 460
EcoT14I CCWWGG 2 cut(s) 309, 446
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 2 cut(s) 309, 446
FaeI CATG 4 cut(s) 272, 347, 409, 430
FaiI YATR 4 cut(s) 270, 345, 407, 428
FaqI GGGAC 1 cut(s) 291
FatI CATG 4 cut(s) 268, 343, 405, 426
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 4 cut(s) 118, 322, 519, 534
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 4 cut(s) 118, 322, 519, 534
FspBI CTAG 1 cut(s) 447
GlaI GCGC 4 cut(s) 358, 454, 508, 532
GluI GCNGC 4 cut(s) 118, 322, 519, 534
HaeII RGCGCY 2 cut(s) 510, 534
HaeIII GGCC 2 cut(s) 5, 397
HapII CCGG 2 cut(s) 254, 393
HgaI GACGC 1 cut(s) 49
HhaI GCGC 4 cut(s) 359, 455, 509, 533
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 4 cut(s) 272, 347, 409, 430
Hin6I GCGC 4 cut(s) 357, 453, 507, 531
HinP1I GCGC 4 cut(s) 357, 453, 507, 531
HinfI GANTC 3 cut(s) 138, 304, 477
HpaII CCGG 2 cut(s) 254, 393
HphI GGTGA 5 cut(s) 178, 301, 310, 382, 472
Hpy166II GTNNAC 3 cut(s) 91, 146, 223
Hpy188I TCNGA 1 cut(s) 476
Hpy188III TCNNGA 4 cut(s) 373, 427, 512, 524
Hpy8I GTNNAC 3 cut(s) 91, 146, 223
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 3 cut(s) 185, 254, 427
HpyCH4V TGCA 3 cut(s) 161, 180, 321
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 330, 515
HpyF3I CTNAG 2 cut(s) 353, 456
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 4 cut(s) 272, 347, 409, 430
HspAI GCGC 4 cut(s) 357, 453, 507, 531
Kzo9I GATC 4 cut(s) 291, 327, 363, 442
LmnI GCTCC 3 cut(s) 469, 514, 526
LpnPI CCDG 6 cut(s) 141, 148, 267, 406, 525, 537
Lsp1109I GCAGC 2 cut(s) 333, 520
LweI GCATC 2 cut(s) 396, 505
MaeI CTAG 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 202
MalI GATC 4 cut(s) 293, 329, 365, 444
MbiI CCGCTC 1 cut(s) 521
MboI GATC 4 cut(s) 291, 327, 363, 442
MboII GAAGA 3 cut(s) 284, 313, 442
MhlI GDGCHC 1 cut(s) 474
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 7, 112, 233, 494
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 313, 471
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 254
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 254, 393
MspR9I CCNGG 1 cut(s) 394
Mva1269I GAATGC 1 cut(s) 182
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 3 cut(s) 114, 330, 515
NciI CCSGG 1 cut(s) 394
NdeII GATC 4 cut(s) 291, 327, 363, 442
NlaIII CATG 4 cut(s) 272, 347, 409, 430
PagI TCATGA 1 cut(s) 426
PctI GAATGC 1 cut(s) 182
PfeI GAWTC 1 cut(s) 138
PkrI GCNGC 4 cut(s) 119, 323, 520, 535
PleI GAGTC 2 cut(s) 312, 471
PpsI GAGTC 2 cut(s) 312, 471
PpuMI RGGWCCY 1 cut(s) 460
Psp124BI GAGCTC 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 460
PspPI GGNCC 2 cut(s) 395, 460
PspPPI RGGWCCY 1 cut(s) 460
PstI CTGCAG 1 cut(s) 323
RseI CAYNNNNRTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 474
SatI GCNGC 4 cut(s) 118, 322, 519, 534
Sau3AI GATC 4 cut(s) 291, 327, 363, 442
Sau96I GGNCC 2 cut(s) 395, 460
SchI GAGTC 2 cut(s) 313, 471
ScrFI CCNGG 1 cut(s) 394
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 2 cut(s) 396, 505
SfcI CTRYAG 1 cut(s) 319
SinI GGWCC 1 cut(s) 460
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 4 cut(s) 7, 112, 233, 494
SsiI CCGC 2 cut(s) 118, 519
SspMI CTAG 1 cut(s) 447
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 392
StyI CCWWGG 2 cut(s) 309, 446
TasI AATT 4 cut(s) 7, 112, 233, 494
TauI GCSGC 2 cut(s) 120, 521
TfiI GAWTC 1 cut(s) 138
TscAI CASTG 1 cut(s) 148
TseI GCWGC 2 cut(s) 321, 533
TspDTI ATGAA 4 cut(s) 285, 332, 415, 443
TspRI CASTG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 1 cut(s) 494
XmaJI CCTAGG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.