Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007375

Physical Location & Seq

Genomic Coordinates Forward (+)

44023178 .. 44029471

Gene Structure 6294 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1309600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3189 bp

ATGAGGAGATTCTTCAGAGGCAAAGACGATTCTTCACCGTCGTCGCCGTCGCCGTCGTTCTCTCCCTCGCCTTCCCGACCGGTGACGGGTCCCCCGAGGCCGATACGGCTCGTCTACTGCGACGAGAATGGGAAGTTCCGGATGGATCAGGAGGCGGTCTCCATGCTGCAGCTCGTTAAGGAACCCATTGGTATCGTCGCGGTCTGCGGCCGTGCCCGCCAGGGCAAGAGCTTTATTCTGAACCAGCTTCTTGGTAGGAGTGGTGGATTTCAAGTAGCATCTACACATCGCCCATGTACGAAAGGGCTATGGTTGTGGAGTGCGCCCTTTAAGAGAATTGCATTGGATGGGACTGAATACAATCTATTACTGTTGGATACTGAAGGAATAGATGCTTTTGATCAGACGGAAACGTACAGCACACAGATATTTTCCTTAGCGGTTCTCCTATCTAGCATGTTTATTTACAACCAGATGGGTGGTATAGATGAAGGTTCACTTGATCGTATCTCTCTTGTCACTCAAATGACCAAGCATATACGTGTTAAAGCTTCTGGAGGAACCACAGCTTCTGAAATTGGGCAGTTCTCCCCAATCTTTGTGTGGCTTCTAAGGGACTTCTATCTGGATTTATCAGAGGAGAGTGGAAAGATAACACCCCGTGATTATCTGGAGCTTGCTTTGAAGCCAGTCGAAGACAGAAAAAGGGACGTAGTTGCTAAAAATGAGATACGAGCCTCCATTCGAGCTCTATTTCCAGACAGAGAATGCTTCACCCTTGTGCGGCCTTTGAACAATGAACATGATCTTCAAAGACTTGACGAAATTCAGTTGGACAAATTAAGGCCTGAATTCAGAGCTGGGCTAGATGCATTGACAAAGTTTGTTTTTGAAAGAACAAGGCCCAAGCAAGTTGGGGCAACAATGATGACAGGTCCAGTTCTAGTTGGTATCACACAGTCTTATCTTGATGCTCTTAATAAAGGGGCGGTGCCTACCATATCCTCTTCATGGCAGAGTGTCGAAGAAGATGGTTGCCGAAGAGCATATGATTCTGCTCTTGAGGTGTATAAGTCTGCTTTTCAGCATCCGAAAGATCCCGAAGAAGCTGCATTCAGGGAAGCACATGAAAAGGCAGTTCAGAAATCATTGGCTGCGTTCAATGATGGTGCTGTAGGAGTTGGTCCACCGAGGAAGAAGTACGAGGCAGAGCTCCACAAAAAGTTGAAAAAGGAGTTTGAGGATTGTAAAAAGAATGCATATATGGAGGCAGAGTTGCACTGTTCAAATGCCATACAAAGCATGGAAAGGAAGCTGAGAACAGCATGCCATGCTAGTGATGCTAATATTGATAATGTTCTTAAGGTTCTTGATGGTCTTCTATCCGACTATGAAGCAGCAAGTCATGGTCCAGCAAAATGGCAGAAACTGACCATGTTCCTACAAAAGAGTTTTGAAGGTCCACTACTTGACCTTGTCAAGAGACTGATTGACCAAGCTGGATCAGAGAAGAGTACCCTCATGTTGAGATGCCGCTCATTAGAAGATAAGTTGGGGTTACTTAACAAGCGGCTGGAGTCCAGTGAGAAGTCTAAGTCTGAATATCTTAAGCGTTATGAGGATGCGATCAATGACAAGAAGAAGCTAGCTGATGAATATATGGGCCGCATAAATAACTTGCAGAGTAATCACAGTTCATTGGGTGAGAGATGTTCTAGTTTGGTTAAAACATTGGATTCTGCAAAGCAGGAATCCTTAGAGTGGAAAAGAAAATATGAACATGTCCTATCAAGGCAGAAGGCTGAGGAAGATCAATGGAGTTCTGAAATTGCAATTCTCAAGTCCCGATGCAGTGCTGGTGAAGCAAGGCTGGCTGCTGCTCGGGAACAAGCTCAGTCTGCACAAGAGGAGGCTGAGGAGTGGAAACGCAAGTATGACATTGCTTTTAGAGAAGCAAAAGCTGCTCTGGAGAAGGCTGCAATTGTCCAAGAGCGTTCAAGTAAGGAAACTCAAAAGAGAGAAGATGCCTTGAGGGAAGAATTTGCTGATACTTTGGCTGATAAGGAGGAGGAAATAAAGGATAAGACGGCAAAGATTGAACATGCTGAGCAGTGCTTGACAACTCTAAGATTAGAATTGAAGGCTGCCCAATCGAAAGTAGATAGTTATGATGCTGAAATATCATCCATGAAGCTTGAAATCAAGGAGTTCATTCAAAAATTAGAAGCTGCAAATGCAAAGGCTCACTCATTTGAGAGAGAGAAAAAGATACTGGAGCAGGAGAAGCTATATCTGGAGCAGAAGTATGAATCTGAGATTAAAAGATTTGACGAAGTGCAAGAAAGGTGCAAAATTGCTGAAAAGGAAGCAACAAGAGCGACTGATATTGCTGATAGAGCACGGGCTCAAGCAGATACTGCTCAGAAGGAAAAGAGTGAGATGCAGAGATTGGCAATGGAAAGATTGGCCCAAATTGAGAGGGCTGAGAGGCTTATTGAAAGTTTGCAAAGGGAAAAAAGAGACGTGGATATTGAACTAGAGAGAATTCGTGTATCAGAGATGGAAGCTCATTCGAAAGTTACTCTACTGGAGGGAAGAGTTGAAGAACGAGAGAAAGAAATAGAATCGCTATTGAAATCAAATAATGAGCAGAGAACTAGTACTGTTCAGGTCCTTCAAGGCCTTCTGGAAAGTGAGCGTGCAGCACATGCAGATGCAAACAATAGGGCAGACCACCTGTCTCATCAGCTACAACTTGCACAATCACAGCTTGATTCACTCCAGCAAGAATTGACTTCTGTTCGGCTGAATGAAACAGCATTGGATAGCAAGCTAAAGACTGCTTCCCATGGGAAACGCTCAAGGGTAGACGACCATGAAGTGGGCATAGACTCTGTTCAAGACATGGAGATGAGTGATCGGATTTTAAGAGTGAATAAGAGGTCTAGAAGTACTACCAGTCCTTTAAAGCACACACAAACAGAAGATGGTGGTTCAGTATTCAAGGGAGATGATGATACCCAGAGTCAGCAAACCAATCCGGAAGACTATAAAAAGTTCACCGTCACAAAAATTAAGCAAGAACTGACAAAACATAATTTTGGTGCCGAAGTGCTCGAGTTGAGAAGCAATGCTAGCAAAAAAGAATGGCTTGATCTGTACGAGAAATGCATCCTTCAGAAGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0008104 GO:0008150 GO:0009987 GO:0010033 GO:0012505 GO:0015629 GO:0017076 GO:0019001 GO:0019221 GO:0023052 GO:0031974 GO:0031981 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0033036 GO:0033365 GO:0034097 GO:0034340 GO:0034341 GO:0034504 GO:0034612 GO:0034613 GO:0035639 GO:0036094 GO:0042221 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046983 GO:0048471 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051641 GO:0051716 GO:0060333 GO:0060337 GO:0065007 GO:0070013 GO:0070555 GO:0070727 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071347 GO:0071356 GO:0071357 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04621 map04621

Pfam Domains

Protein Families

GBP GBP_C

Protein Analysis

1063

Amino Acids

120.83

Weight (kDa)

6.33

Isoelectric Point (pI)

49.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GBP	PF02263	44 - 300	1.1e-60	Guanylate-binding protein, N-terminal domain
GBP_C	PF02841	306 - 609	8.7e-32	Guanylate-binding protein, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46070
fragaria_vesca	FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471
malus_domestica	MD05G1309600.v1.1 MD10G1288200.v1.1
prunus_persica	Prupe.4G053400_v2.0.a1
pyrus_communis	pycom05g28600 pycom10g24130 pycom10g24140
rosa_chinensis	RchiOBHm_Chr5g0009341
rosa_laevigata	RLG00000031653
rosa_multiflora	Rmu_sc0002711.1_g000023 Rmu_sc0009480.1_g000002
rosa_roxburghii	Rroxscaffold_1G00066750 Rroxscaffold_1G00067450
rosa_rugosa	Rorug04G0441800
rosa_samantha	Rh5AG072700 Rh5BG069000 Rh5CG081600 Rh5DG069300
rosa_wichuraiana	Rw5G006790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	991, 3104
AccB7I	CCANNNNNTGG	1 cut(s)	2881
AccBSI	CCGCTC	1 cut(s)	1536
AccI	GTMKAC	2 cut(s)	114, 2868
AccII	CGCG	1 cut(s)	200
AccIII	TCCGGA	2 cut(s)	138, 3040
AclWI	GGATC	3 cut(s)	153, 1091, 1510
AcoI	YGGCCR	1 cut(s)	208
AcsI	RAATTY	4 cut(s)	825, 851, 2039, 2544
AcuI	CTGAAG	2 cut(s)	402, 3161
AdeI	CACNNNGTG	1 cut(s)	662
AfaI	GTAC	7 cut(s)	298, 416, 1202, 1516, 2662, 2953, 3161
AfiI	CCNNNNNNNGG	5 cut(s)	86, 783, 1011, 1761, 2881
AflII	CTTAAG	2 cut(s)	1361, 1607
AflIII	ACRYGT	2 cut(s)	541, 1780
AgeI	ACCGGT	1 cut(s)	79
AhdI	GACNNNNNGTC	1 cut(s)	2737
AhlI	ACTAGT	1 cut(s)	2657
AjiI	CACGTC	1 cut(s)	2524
AjnI	CCWGG	1 cut(s)	219
AleI	CACNNNNGTG	1 cut(s)	779
Alw21I	GWGCWC	4 cut(s)	751, 1215, 2401, 3117
Alw26I	GTCTC	4 cut(s)	163, 1477, 2514, 2745
AlwI	GGATC	3 cut(s)	153, 1091, 1510
AlwNI	CAGNNNCTG	3 cut(s)	572, 1429, 2417
Ama87I	CYCGRG	3 cut(s)	94, 1881, 3116
Aor13HI	TCCGGA	2 cut(s)	138, 3040
AoxI	GGCC	8 cut(s)	98, 208, 785, 845, 902, 1663, 2466, 2680
ApoI	RAATTY	4 cut(s)	825, 851, 2039, 2544
AsiGI	ACCGGT	1 cut(s)	79
AspLEI	GCGC	1 cut(s)	325
AspS9I	GGNCC	9 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2467, 2671
AsuHPI	GGTGA	6 cut(s)	27, 94, 766, 1715, 1871, 3052
AsuII	TTCGAA	1 cut(s)	2573
AsuNHI	GCTAGC	2 cut(s)	1645, 3134
AvaI	CYCGRG	3 cut(s)	94, 1881, 3116
AvaII	GGWCC	6 cut(s)	89, 935, 1184, 1409, 1460, 2671
BaeGI	GKGCMC	1 cut(s)	217
BanI	GGYRCC	2 cut(s)	991, 3104
BanII	GRGCYC	3 cut(s)	751, 1215, 2407
BarI	GAAGNNNNNNTAC	2 cut(s)	1449, 1481
BbsI	GAAGAC	3 cut(s)	702, 1370, 3051
Bbv12I	GWGCWC	4 cut(s)	751, 1215, 2401, 3117
BbvCI	CCTCAGC	2 cut(s)	1803, 1914
BccI	CCATC	8 cut(s)	136, 341, 469, 1025, 1160, 1367, 2554, 2981
BceAI	ACGGC	5 cut(s)	31, 37, 122, 195, 2103
BcgI	CGANNNNNNTGC	2 cut(s)	1091, 1125
BciT130I	CCWGG	1 cut(s)	221
BciVI	GTATCC	1 cut(s)	370
BclI	TGATCA	1 cut(s)	400
BcoDI	GTCTC	4 cut(s)	163, 1477, 2514, 2745
BcuI	ACTAGT	1 cut(s)	2657
BfmI	CTRYAG	2 cut(s)	167, 1173
BfrI	CTTAAG	2 cut(s)	1361, 1607
BfuI	GTATCC	1 cut(s)	370
BglI	GCCNNNNNGGC	1 cut(s)	106
BlpI	GCTNAGC	1 cut(s)	2106
BmcAI	AGTACT	2 cut(s)	2662, 2953
Bme1390I	CCNGG	1 cut(s)	221
Bme18I	GGWCC	6 cut(s)	89, 935, 1184, 1409, 1460, 2671
BmeRI	GACNNNNNGTC	1 cut(s)	2737
BmeT110I	CYCGRG	3 cut(s)	94, 1881, 3116
BmgBI	CACGTC	1 cut(s)	2524
BmgT120I	GGNCC	9 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2467, 2671
BmiI	GGNNCC	6 cut(s)	90, 91, 183, 562, 993, 3106
BmrFI	CCNGG	1 cut(s)	221
BmtI	GCTAGC	2 cut(s)	1649, 3138
BpiI	GAAGAC	3 cut(s)	702, 1370, 3051
BplI	GAGNNNNNCTC	2 cut(s)	143, 175
BpmI	CTGGAG	8 cut(s)	576, 692, 1595, 1988, 2294, 2315, 2609, 2765
Bpu10I	CCTNAGC	3 cut(s)	436, 1803, 1914
Bpu1102I	GCTNAGC	1 cut(s)	2106
Bpu14I	TTCGAA	1 cut(s)	2573
BpuEI	CTTGAG	5 cut(s)	1082, 1823, 2050, 2391, 2845
BsaAI	YACGTR	1 cut(s)	542
BsaI	GGTCTC	1 cut(s)	163
BsaJI	CCNNGG	4 cut(s)	95, 220, 1190, 2848
BsaWI	WCCGGW	3 cut(s)	79, 138, 3040
BsaXI	ACNNNNNCTCC	4 cut(s)	1259, 1289, 2288, 2318
Bsc4I	CCNNNNNNNGG	5 cut(s)	86, 783, 1011, 1761, 2881
Bse118I	RCCGGY	1 cut(s)	79
Bse1I	ACTGG	6 cut(s)	689, 938, 1581, 2277, 2592, 2958
Bse3DI	GCAATG	3 cut(s)	1938, 2460, 3136
BseAI	TCCGGA	2 cut(s)	138, 3040
BseBI	CCWGG	1 cut(s)	221
BseDI	CCNNGG	4 cut(s)	95, 220, 1190, 2848
BseGI	GGATG	6 cut(s)	147, 352, 1087, 1627, 2183, 3171
BseLI	CCNNNNNNNGG	5 cut(s)	86, 783, 1011, 1761, 2881
BseMI	GCAATG	3 cut(s)	1938, 2460, 3136
BseMII	CTCAG	8 cut(s)	1307, 1794, 1905, 1907, 2097, 2304, 2435, 2475
BseNI	ACTGG	6 cut(s)	689, 938, 1581, 2277, 2592, 2958
BseRI	GAGGAG	5 cut(s)	19, 653, 1922, 1931, 2081
BseSI	GKGCMC	1 cut(s)	217
BseX3I	CGGCCG	1 cut(s)	208
BseYI	CCCAGC	1 cut(s)	860
BsgI	GTGCAG	2 cut(s)	1884, 2721
Bsh1236I	CGCG	1 cut(s)	200
Bsh1285I	CGRYCG	2 cut(s)	80, 211
BshFI	GGCC	8 cut(s)	100, 210, 787, 847, 904, 1665, 2468, 2682
BshNI	GGYRCC	2 cut(s)	991, 3104
BshTI	ACCGGT	1 cut(s)	79
BsiEI	CGRYCG	2 cut(s)	80, 211
BsiHKAI	GWGCWC	4 cut(s)	751, 1215, 2401, 3117
BsiHKCI	CYCGRG	3 cut(s)	94, 1881, 3116
BsiSI	CCGG	3 cut(s)	80, 139, 3041
BslFI	GGGAC	5 cut(s)	75, 364, 629, 722, 1828
BslI	CCNNNNNNNGG	5 cut(s)	86, 783, 1011, 1761, 2881
BsmAI	GTCTC	4 cut(s)	163, 1477, 2514, 2745
BsmBI	CGTCTC	1 cut(s)	2514
BsmFI	GGGAC	5 cut(s)	75, 364, 629, 722, 1828
BsmI	GAATGC	3 cut(s)	773, 1112, 1261
BsnI	GGCC	8 cut(s)	100, 210, 787, 847, 904, 1665, 2468, 2682
Bso31I	GGTCTC	1 cut(s)	163
BsoBI	CYCGRG	3 cut(s)	94, 1881, 3116
Bsp119I	TTCGAA	1 cut(s)	2573
Bsp1286I	GDGCHC	6 cut(s)	217, 751, 1215, 2401, 2407, 3117
Bsp13I	TCCGGA	2 cut(s)	138, 3040
Bsp1720I	GCTNAGC	1 cut(s)	2106
Bsp19I	CCATGG	1 cut(s)	2848
BspANI	GGCC	8 cut(s)	100, 210, 787, 847, 904, 1665, 2468, 2682
BspCNI	CTCAG	8 cut(s)	1308, 1795, 1906, 1906, 2098, 2305, 2434, 2476
BspEI	TCCGGA	2 cut(s)	138, 3040
BspFNI	CGCG	1 cut(s)	200
BspLI	GGNNCC	6 cut(s)	90, 91, 183, 562, 993, 3106
BspMAI	CTGCAG	1 cut(s)	171
BspOI	GCTAGC	2 cut(s)	1649, 3138
BspPI	GGATC	3 cut(s)	153, 1091, 1510
BspQI	GCTCTTC	1 cut(s)	1036
BspT104I	TTCGAA	1 cut(s)	2573
BspT107I	GGYRCC	2 cut(s)	991, 3104
BspTI	CTTAAG	2 cut(s)	1361, 1607
BspTNI	GGTCTC	1 cut(s)	163
BsrBI	CCGCTC	1 cut(s)	1536
BsrDI	GCAATG	3 cut(s)	1938, 2460, 3136
BsrFI	RCCGGY	1 cut(s)	79
BsrI	ACTGG	6 cut(s)	689, 938, 1581, 2277, 2592, 2958
BssAI	RCCGGY	1 cut(s)	79
BssECI	CCNNGG	4 cut(s)	95, 220, 1190, 2848
BssT1I	CCWWGG	1 cut(s)	2848
Bst2UI	CCWGG	1 cut(s)	221
Bst4CI	ACNGT	7 cut(s)	39, 372, 960, 1283, 1694, 2665, 3064
Bst6I	CTCTTC	4 cut(s)	1012, 1036, 1505, 2590
BstAFI	CTTAAG	2 cut(s)	1361, 1607
BstAPI	GCANNNNNTGC	4 cut(s)	1331, 1961, 2417, 2708
BstBAI	YACGTR	1 cut(s)	542
BstBI	TTCGAA	1 cut(s)	2573
BstC8I	GCNNGC	8 cut(s)	217, 678, 1327, 1647, 1872, 2700, 2831, 3136
BstDSI	CCRYGG	1 cut(s)	2848
BstF5I	GGATG	6 cut(s)	147, 352, 1087, 1627, 2183, 3171
BstFNI	CGCG	1 cut(s)	200
BstHHI	GCGC	1 cut(s)	325
BstMAI	GTCTC	4 cut(s)	163, 1477, 2514, 2745
BstMCI	CGRYCG	2 cut(s)	80, 211
BstNI	CCWGG	1 cut(s)	221
BstNSI	RCATGY	5 cut(s)	460, 1329, 1784, 2105, 2711
BstSCI	CCNGG	1 cut(s)	219
BstSFI	CTRYAG	2 cut(s)	167, 1173
BstSLI	GKGCMC	1 cut(s)	217
BstUI	CGCG	1 cut(s)	200
BstV2I	GAAGAC	3 cut(s)	702, 1370, 3051
BstX2I	RGATCY	1 cut(s)	1096
BstXI	CCANNNNNNTGG	3 cut(s)	251, 479, 1419
BstYI	RGATCY	1 cut(s)	1096
BstZI	CGGCCG	1 cut(s)	208
BsuI	GTATCC	1 cut(s)	370
BsuRI	GGCC	8 cut(s)	100, 210, 787, 847, 904, 1665, 2468, 2682
BtgI	CCRYGG	1 cut(s)	2848
BtgZI	GCGATG	1 cut(s)	272
BtrI	CACGTC	1 cut(s)	2524
BtsCI	GGATG	6 cut(s)	147, 352, 1087, 1627, 2183, 3171
BtsI	GCAGTG	2 cut(s)	1858, 2117
BtsIMutI	CAGTG	4 cut(s)	1279, 1588, 1858, 2117
Cac8I	GCNNGC	8 cut(s)	217, 678, 1327, 1647, 1872, 2700, 2831, 3136
CaiI	CAGNNNCTG	3 cut(s)	572, 1429, 2417
CfoI	GCGC	1 cut(s)	325
Cfr10I	RCCGGY	1 cut(s)	79
Cfr13I	GGNCC	9 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2467, 2671
Csp6I	GTAC	7 cut(s)	297, 415, 1201, 1515, 2661, 2952, 3160
CspAI	ACCGGT	1 cut(s)	79
CviQI	GTAC	7 cut(s)	297, 415, 1201, 1515, 2661, 2952, 3160
DraI	TTTAAA	1 cut(s)	2967
DraIII	CACNNNGTG	1 cut(s)	662
DriI	GACNNNNNGTC	1 cut(s)	2737
EaeI	YGGCCR	1 cut(s)	208
EagI	CGGCCG	1 cut(s)	208
Eam1104I	CTCTTC	4 cut(s)	1012, 1036, 1505, 2590
Eam1105I	GACNNNNNGTC	1 cut(s)	2737
EarI	CTCTTC	4 cut(s)	1012, 1036, 1505, 2590
Ecl136II	GAGCTC	2 cut(s)	749, 1213
EclXI	CGGCCG	1 cut(s)	208
Eco130I	CCWWGG	1 cut(s)	2848
Eco147I	AGGCCT	2 cut(s)	847, 2682
Eco24I	GRGCYC	3 cut(s)	751, 1215, 2407
Eco31I	GGTCTC	1 cut(s)	163
Eco47I	GGWCC	6 cut(s)	89, 935, 1184, 1409, 1460, 2671
Eco52I	CGGCCG	1 cut(s)	208
Eco53kI	GAGCTC	2 cut(s)	749, 1213
Eco57I	CTGAAG	2 cut(s)	402, 3161
Eco88I	CYCGRG	3 cut(s)	94, 1881, 3116
EcoICRI	GAGCTC	2 cut(s)	749, 1213
EcoO109I	RGGNCCY	2 cut(s)	89, 2671
EcoRI	GAATTC	2 cut(s)	851, 2544
EcoRII	CCWGG	1 cut(s)	219
EcoT14I	CCWWGG	1 cut(s)	2848
EcoT22I	ATGCAT	3 cut(s)	874, 1261, 3173
EcoT38I	GRGCYC	3 cut(s)	751, 1215, 2407
ErhI	CCWWGG	1 cut(s)	2848
Esp3I	CGTCTC	1 cut(s)	2514
FalI	AAGNNNNNCTT	4 cut(s)	483, 515, 3135, 3167
FaqI	GGGAC	5 cut(s)	75, 364, 629, 722, 1828
FauI	CCCGC	1 cut(s)	224
FauNDI	CATATG	1 cut(s)	1048
FbaI	TGATCA	1 cut(s)	400
FblI	GTMKAC	2 cut(s)	114, 2868
FokI	GGATG	6 cut(s)	154, 359, 1074, 1634, 2170, 3158
FriOI	GRGCYC	3 cut(s)	751, 1215, 2407
GlaI	GCGC	1 cut(s)	324
GsaI	CCCAGC	1 cut(s)	864
GsuI	CTGGAG	8 cut(s)	576, 692, 1595, 1988, 2294, 2315, 2609, 2765
HaeIII	GGCC	8 cut(s)	100, 210, 787, 847, 904, 1665, 2468, 2682
HapII	CCGG	3 cut(s)	80, 139, 3041
HhaI	GCGC	1 cut(s)	325
Hin6I	GCGC	1 cut(s)	323
HinP1I	GCGC	1 cut(s)	323
HindIII	AAGCTT	2 cut(s)	549, 2192
HpaII	CCGG	3 cut(s)	80, 139, 3041
HphI	GGTGA	6 cut(s)	27, 94, 766, 1715, 1871, 3052
Hpy166II	GTNNAC	6 cut(s)	115, 497, 1187, 1463, 2869, 3060
Hpy8I	GTNNAC	6 cut(s)	115, 497, 1187, 1463, 2869, 3060
Hpy99I	CGWCG	6 cut(s)	43, 46, 52, 58, 125, 200
HpyCH4III	ACNGT	7 cut(s)	39, 372, 960, 1283, 1694, 2665, 3064
HpyCH4IV	ACGT	4 cut(s)	413, 541, 711, 2523
HpySE526I	ACGT	4 cut(s)	413, 541, 711, 2523
HspAI	GCGC	1 cut(s)	323
KflI	GGGWCCC	1 cut(s)	89
Kpn2I	TCCGGA	2 cut(s)	138, 3040
Ksp22I	TGATCA	1 cut(s)	400
LguI	GCTCTTC	1 cut(s)	1036
LmnI	GCTCC	4 cut(s)	673, 1218, 2275, 2296
MaeII	ACGT	4 cut(s)	413, 541, 711, 2523
MaeIII	GTNAC	5 cut(s)	82, 517, 1557, 2578, 3064
MbiI	CCGCTC	1 cut(s)	1536
MfeI	CAATTG	1 cut(s)	1980
MflI	RGATCY	1 cut(s)	1096
MhlI	GDGCHC	6 cut(s)	217, 751, 1215, 2401, 2407, 3117
MlyI	GAGTC	3 cut(s)	1586, 2885, 3034
MmeI	TCCRAC	3 cut(s)	354, 813, 1410
Mph1103I	ATGCAT	3 cut(s)	874, 1261, 3173
MroI	TCCGGA	2 cut(s)	138, 3040
MslI	CAYNNNNRTG	6 cut(s)	524, 540, 779, 1335, 2712, 2909
MspCI	CTTAAG	2 cut(s)	1361, 1607
MspI	CCGG	3 cut(s)	80, 139, 3041
MspR9I	CCNGG	1 cut(s)	221
MunI	CAATTG	1 cut(s)	1980
Mva1269I	GAATGC	3 cut(s)	773, 1112, 1261
MvaI	CCWGG	1 cut(s)	221
MvnI	CGCG	1 cut(s)	200
NcoI	CCATGG	1 cut(s)	2848
NdeI	CATATG	1 cut(s)	1048
NheI	GCTAGC	2 cut(s)	1645, 3134
NlaIV	GGNNCC	6 cut(s)	90, 91, 183, 562, 993, 3106
NmuCI	GTSAC	3 cut(s)	82, 517, 3064
NsiI	ATGCAT	3 cut(s)	874, 1261, 3173
NspI	RCATGY	5 cut(s)	460, 1329, 1784, 2105, 2711
NspV	TTCGAA	1 cut(s)	2573
OliI	CACNNNNGTG	1 cut(s)	779
PaeI	GCATGC	1 cut(s)	1329
PaeR7I	CTCGAG	1 cut(s)	3116
PceI	AGGCCT	2 cut(s)	847, 2682
PciI	ACATGT	1 cut(s)	1780
PciSI	GCTCTTC	1 cut(s)	1036
PcsI	WCGNNNNNNNCGW	3 cut(s)	50, 92, 117
PctI	GAATGC	3 cut(s)	773, 1112, 1261
PfeI	GAWTC	8 cut(s)	9, 29, 1052, 1736, 1751, 2309, 2624, 2774
PflFI	GACNNNGTC	1 cut(s)	1475
PflMI	CCANNNNNTGG	1 cut(s)	2881
PinAI	ACCGGT	1 cut(s)	79
PleI	GAGTC	3 cut(s)	1585, 2885, 3033
PpsI	GAGTC	3 cut(s)	1585, 2885, 3033
Ppu21I	YACGTR	1 cut(s)	542
PpuMI	RGGWCCY	2 cut(s)	89, 2671
PscI	ACATGT	1 cut(s)	1780
Psp124BI	GAGCTC	2 cut(s)	751, 1215
Psp5II	RGGWCCY	2 cut(s)	89, 2671
Psp6I	CCWGG	1 cut(s)	219
PspFI	CCCAGC	1 cut(s)	860
PspGI	CCWGG	1 cut(s)	219
PspN4I	GGNNCC	6 cut(s)	90, 91, 183, 562, 993, 3106
PspPI	GGNCC	9 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2467, 2671
PspPPI	RGGWCCY	2 cut(s)	89, 2671
PspXI	VCTCGAGB	1 cut(s)	3116
PsrI	GAACNNNNNNTAC	2 cut(s)	1678, 1710
PstI	CTGCAG	1 cut(s)	171
PstNI	CAGNNNCTG	3 cut(s)	572, 1429, 2417
PsuI	RGATCY	1 cut(s)	1096
PsyI	GACNNNGTC	1 cut(s)	1475
RsaI	GTAC	7 cut(s)	298, 416, 1202, 1516, 2662, 2953, 3161
RsaNI	GTAC	7 cut(s)	297, 415, 1201, 1515, 2661, 2952, 3160
RseI	CAYNNNNRTG	6 cut(s)	524, 540, 779, 1335, 2712, 2909
SacI	GAGCTC	2 cut(s)	751, 1215
SapI	GCTCTTC	1 cut(s)	1036
Sau96I	GGNCC	9 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2467, 2671
ScaI	AGTACT	2 cut(s)	2662, 2953
SchI	GAGTC	3 cut(s)	1586, 2885, 3034
ScrFI	CCNGG	1 cut(s)	221
SduI	GDGCHC	6 cut(s)	217, 751, 1215, 2401, 2407, 3117
SfcI	CTRYAG	2 cut(s)	167, 1173
Sfr274I	CTCGAG	1 cut(s)	3116
SfuI	TTCGAA	1 cut(s)	2573
SinI	GGWCC	6 cut(s)	89, 935, 1184, 1409, 1460, 2671
SlaI	CTCGAG	1 cut(s)	3116
SmiMI	CAYNNNNRTG	6 cut(s)	524, 540, 779, 1335, 2712, 2909
SmlI	CTYRAG	8 cut(s)	1061, 1361, 1607, 1838, 2029, 2406, 2860, 3116
SmoI	CTYRAG	8 cut(s)	1061, 1361, 1607, 1838, 2029, 2406, 2860, 3116
SpeI	ACTAGT	1 cut(s)	2657
SphI	GCATGC	1 cut(s)	1329
SseBI	AGGCCT	2 cut(s)	847, 2682
SspI	AATATT	1 cut(s)	1348
SstI	GAGCTC	2 cut(s)	751, 1215
StuI	AGGCCT	2 cut(s)	847, 2682
StyD4I	CCNGG	1 cut(s)	219
StyI	CCWWGG	1 cut(s)	2848
TaaI	ACNGT	7 cut(s)	39, 372, 960, 1283, 1694, 2665, 3064
TaiI	ACGT	4 cut(s)	416, 544, 714, 2526
TaqI	TCGA	6 cut(s)	693, 745, 1023, 2153, 2573, 3117
TatI	WGTACW	2 cut(s)	2660, 2951
TauI	GCSGC	5 cut(s)	210, 787, 1536, 1573, 1668
TfiI	GAWTC	8 cut(s)	9, 29, 1052, 1736, 1751, 2309, 2624, 2774
TscAI	CASTG	4 cut(s)	1286, 1588, 1858, 2117
TseFI	GTSAC	3 cut(s)	82, 517, 3064
Tsp45I	GTSAC	3 cut(s)	82, 517, 3064
TspGWI	ACGGA	1 cut(s)	422
TspRI	CASTG	4 cut(s)	1286, 1588, 1858, 2117
Tth111I	GACNNNGTC	1 cut(s)	1475
Van91I	CCANNNNNTGG	1 cut(s)	2881
Vha464I	CTTAAG	2 cut(s)	1361, 1607
VpaK11BI	GGWCC	6 cut(s)	89, 935, 1184, 1409, 1460, 2671
XapI	RAATTY	4 cut(s)	825, 851, 2039, 2544
XbaI	TCTAGA	1 cut(s)	2945
XceI	RCATGY	5 cut(s)	460, 1329, 1784, 2105, 2711
XcmI	CCANNNNNNNNNTGG	2 cut(s)	600, 1300
XhoI	CTCGAG	1 cut(s)	3116
XmiI	GTMKAC	2 cut(s)	114, 2868
ZrmI	AGTACT	2 cut(s)	2662, 2953
Zsp2I	ATGCAT	3 cut(s)	874, 1261, 3173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.