Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007375

Physical Location & Seq

Genomic Coordinates Forward (+)

37742129 .. 37748406

Gene Structure 6278 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1288200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3183 bp

ATGATGAAATTCTTCAGAGGCAAAGACGATTCTTCACCGCCGTCGCCGTCGCAGTCGTTCTCTCCCTCGCCTTCGCGGTCGGTGACCGGTCCCCCGAGGCCGATACGGCTCGTCTACTGCGACGAGAATGGGAAGTTCCGGATGGATCAGGAGGCTGTCTCCATGCTGCAGCTCGTAAAGGAACCCATTGGTATCGTCGCGGTCTGCGGCCGTGCCCGCCAGGGCAAGAGCTTTATTCTGAACCAGCTCCTCGGAAGAAGTGGCGGATTTCAAGTAGCATCTACACATCGCCCATGTACAAAAGGGCTATGGTTGTGGAGTGCACCCTTTAAGAGAACTGCACTGGATGGGACTGAATACAATCTATTACTATTGGATACTGAAGGAATAGATGCTTTTGATCAGACGGAAACGTACAGCACACAGATATTTTCCTTAGCAGTTCTCCTATCTAGCATGTTTATTTACAACCAGATGGGTGGTATAGATGAAGGTTCCCTCGATCGTATCTCTCTTGTCACTCAAATGTCCAAGCATATACGTGTTAAAGCTTCTGGAGGAACCACAGCTTCTGAAATTGGGCAGTTCTCCCCAATCTTTGTGTGGCTTCTAAGGGACTTCTATCTGAATTTATCAGAGGATAGTGGAAGGATAACACCTCGTGATTATCTGGAGCTTGCTTTGAAGCCAGTCGAAGACAGAAAAAGGGACGTAGTTGCTAAAAATGAGATACGAGCCTCCATTCGAGCTTTATTTCCAGACAGAGAATGCTTCACCCTTGTGCGGCCTTTGAATGACGAACATGATCTTCAAAGACTTGACGAAATTCGGTTGGATAAATTAAGGCCTGAATTCAGAGCCGGGCTAGATGCATTGACAAAGTTTGTTTTTGAAAGAACAAGGCCCAAGCAAGTTGGGGCAACAATGATGACAGGTCCAGTTCTAGTTGGTATCACACAGTCTTATCTTGATGCTCTTAATAAAGGCGCGGTGCCTACCATATCCTCTTCATGGCAGAGTGTTGAAGAAGATGGTTGCCGAAGAGCATATGATTCTGCTCTTGAGGTGTATAAGTCTGCTTTTCAGCGTCCAAAAGATCCCGAAGAAGCTGCATTCAGGGAAGCACATGAAAAGGCAGTTCATAGATCATTGGCTGCGTTCAATGATGGTGCTGTAGGAGTTGGTCCACCAAGGAAGAAGTATGAGGCGGAGCTCCACAAAAAGTTGAAAAAGGAGTTTGAGGATTGTAAAAAGAATGCATATATGGAGGCAGAGTTGCACTGTTCAAATGCCATACAAATCATGGAAAGGAAGCTGAGAACAGCATGCCATGCTAGTGATGCTAATATTGATAATGTTGGGAAGGTTCTTGATGGTCTTCTATCCGACTATGAAGCAGCAAGTCATGGTCCAGCAAAATGGCAGAAACTGACCATGTTCTTACAGAAGAGTTTTGAAGGGCCAATACTTGACCTCGTCAAGAGACTGATTGACCAAGCTGGGTCAGAGAAGAATACCCTCATGTTGAGGTGCCGCTCATTAGAAGATAAGTTGGGGTTACTTAACAAGCAGCTGGAGTCTAGTGAGAAGTCGAAGTCTGAATATCTTAAGCGTTATGAGGATGCTATCAATGACAAGAAGAAGCTAGCTGATGAATATATGGGCCGCATAAATAACTTGCAGAGTAATCACAGTTCATTGGGTGAGAGATGTTCTGGCTTGATTAAAACATTGGATTCTGCAAAGCAGGAATCCTTAGAGTGGAAAAGAAAATATGAACATGTCTTATCAAGGCAGAAGGCTGAGGAAGATCAATGGAGTTCTGAAATTGCAATTCTCAAGTCCCGATGCAGTGCTGGTGAAGCAAGGCTGGCTGCTGCTCGGGAACAAGCTCAGTCTGCTCAAGAGGAGGCTGAGGAGTGGAAACGCAAGTATGACAATGCTTTTAGAGAAGCAAAAGCTGCTCTGGAGAAGGCTGCAACTGTCCAAGAGCGTTCAAGTAAGGAAACTCAGAAGAGAGAAGATGCTTTGAGGGCAGAATTCGCTGATACTCTGGCTGATAAGGAGGAGGAAATAAAGGATAAGACGGCAAAGATTGAACATGCAGAGCAGTGCTTGACAACTCTAAGGTTAGAATTGAAGGCTGCCCAATCGAAAGTAGATAGCTATGATGCTGAAACATCATCCATGAAGCTTGAAATAAAGGAGTTAAGTAAAAAATTAGAAGCTACAAATGCAAAGGCCCACTCATTTGAGAGAGAGAAAAAGATACTGGAGCAGGAGAAGATATATCTGGAGCAGAAGTACGAATCTGATATAAAAAGATTTGACGAAGTGCAAGAAAGGTGCAAAATTGCTGAAAAGGAAGCAACAAGAGCAACTGATATTGCTGATAGAGCACGGGCTCAAGCAGATACTGCTCAGAAGGAAAAGAGTGAGATGCAGAGATTGGCAATGGAAAGATTGGCTCAAATTGAGAGGGCTGAGAGGCTTATTGAAAGTTTGCAAAGGGAAAAAAGAGACTTGGATAATGAACTAGACAGAATTCGTGTATCAGAGATGGAAGCTCATTCGAAAGTTACCCTACTGGAGGGAAGAGTTGAAGAACGAGAGAAAGAAATAGAATTGCTTTTGAAATCAAATAACGAGCAGAGAACTAGTACTGTTCAGGTTCTTCAAGGCCTTCTGGAAAGTGAGCGCGCAGCACATGCAGATGCAAACAATAGGGCAGACCACCTCTCTCATCAGCTACAACTTGCACAATCACAACTGGATTCACTCCAGCAAGAATTGACTTCTGTTCGGCTGAATGAAACAGCATTGGATAGCAAGTTAAGGACTACTTCCCGTGGGAAACGCTCAAGGGTAGACGACTATGAAATGGGCATGGACTCTGTTCAAGACGTGGAGATGAGTGATCGGATTTTAAGAGTGAATAAGAGGTCTAGAAGTACTACCAGTCCGCACACACAAAAAGAAGATGGTGGTTCAGTATTCAAGGGAGATGATGATACCCGCAGTCAGCAAACCAATTCGGAAGACTATAAAAAGTTCACCGTTGCAAAAATTAAGCAAGAACTCACAAAACATAATTTTGGTGCCGAAGTGCTCGAGTTGAGAAGCAATGCTAGCAAAAAAGATTGGCTTGATCTGTACGAGAAATGCATCCTCCAGAAGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0008104 GO:0008150 GO:0009987 GO:0010033 GO:0012505 GO:0015629 GO:0017076 GO:0019001 GO:0019221 GO:0023052 GO:0031974 GO:0031981 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0033036 GO:0033365 GO:0034097 GO:0034340 GO:0034341 GO:0034504 GO:0034612 GO:0034613 GO:0035639 GO:0036094 GO:0042221 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046983 GO:0048471 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051641 GO:0051716 GO:0060333 GO:0060337 GO:0065007 GO:0070013 GO:0070555 GO:0070727 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071347 GO:0071356 GO:0071357 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04621 map04621

Pfam Domains

Protein Families

GBP GBP_C

Protein Analysis

1061

Amino Acids

120.62

Weight (kDa)

6.63

Isoelectric Point (pI)

48.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GBP	PF02263	44 - 300	6.9e-60	Guanylate-binding protein, N-terminal domain
GBP_C	PF02841	306 - 609	7.1e-31	Guanylate-binding protein, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46070
fragaria_vesca	FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471
malus_domestica	MD05G1309600.v1.1 MD10G1288200.v1.1
prunus_persica	Prupe.4G053400_v2.0.a1
pyrus_communis	pycom05g28600 pycom10g24130 pycom10g24140
rosa_chinensis	RchiOBHm_Chr5g0009341
rosa_laevigata	RLG00000031653
rosa_multiflora	Rmu_sc0002711.1_g000023 Rmu_sc0009480.1_g000002
rosa_roxburghii	Rroxscaffold_1G00066750 Rroxscaffold_1G00067450
rosa_rugosa	Rorug04G0441800
rosa_samantha	Rh5AG072700 Rh5BG069000 Rh5CG081600 Rh5DG069300
rosa_wichuraiana	Rw5G006790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	991, 1530, 3098
AccBSI	CCGCTC	1 cut(s)	1536
AccI	GTMKAC	2 cut(s)	114, 2868
AccII	CGCG	4 cut(s)	76, 200, 989, 2700
AccIII	TCCGGA	1 cut(s)	138
AclWI	GGATC	2 cut(s)	153, 1091
AcoI	YGGCCR	1 cut(s)	208
AcsI	RAATTY	6 cut(s)	8, 628, 825, 851, 2039, 2544
AcuI	CTGAAG	1 cut(s)	402
AdeI	CACNNNGTG	1 cut(s)	662
AfaI	GTAC	6 cut(s)	298, 416, 2306, 2662, 2953, 3155
AfiI	CCNNNNNNNGG	4 cut(s)	783, 1011, 1761, 2590
AflII	CTTAAG	1 cut(s)	1607
AflIII	ACRYGT	2 cut(s)	541, 1780
AgeI	ACCGGT	1 cut(s)	86
AhlI	ACTAGT	1 cut(s)	2657
AjiI	CACGTC	1 cut(s)	2905
AjnI	CCWGG	1 cut(s)	219
AleI	CACNNNNGTG	1 cut(s)	779
Alw21I	GWGCWC	4 cut(s)	325, 1215, 2401, 3111
Alw26I	GTCTC	3 cut(s)	163, 1477, 2514
Alw44I	GTGCAC	1 cut(s)	321
AlwI	GGATC	2 cut(s)	153, 1091
AlwNI	CAGNNNCTG	3 cut(s)	572, 1429, 2417
Ama87I	CYCGRG	3 cut(s)	94, 1881, 3110
Aor13HI	TCCGGA	1 cut(s)	138
AoxI	GGCC	9 cut(s)	98, 208, 785, 845, 902, 1460, 1663, 2241, 2680
ApaLI	GTGCAC	1 cut(s)	321
ApoI	RAATTY	6 cut(s)	8, 628, 825, 851, 2039, 2544
AsiGI	ACCGGT	1 cut(s)	86
Asp700I	GAANNNNTTC	1 cut(s)	11
AspLEI	GCGC	3 cut(s)	989, 2700, 2702
AspS9I	GGNCC	8 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2242
AsuC2I	CCSGG	1 cut(s)	862
AsuHPI	GGTGA	6 cut(s)	27, 94, 766, 1715, 1871, 3046
AsuII	TTCGAA	1 cut(s)	2573
AsuNHI	GCTAGC	2 cut(s)	1645, 3128
AvaI	CYCGRG	3 cut(s)	94, 1881, 3110
AvaII	GGWCC	4 cut(s)	89, 935, 1184, 1409
BaeGI	GKGCMC	2 cut(s)	217, 325
BanI	GGYRCC	3 cut(s)	991, 1530, 3098
BanII	GRGCYC	2 cut(s)	1215, 2407
BarI	GAAGNNNNNNTAC	2 cut(s)	1449, 1481
BauI	CACGAG	1 cut(s)	660
BbsI	GAAGAC	3 cut(s)	702, 1370, 3045
Bbv12I	GWGCWC	4 cut(s)	325, 1215, 2401, 3111
BbvCI	CCTCAGC	2 cut(s)	1803, 1914
BccI	CCATC	8 cut(s)	136, 341, 469, 1025, 1160, 1367, 2554, 2975
BceAI	ACGGC	5 cut(s)	25, 31, 122, 195, 2103
BcgI	CGANNNNNNTGC	2 cut(s)	1091, 1125
BciT130I	CCWGG	1 cut(s)	221
BciVI	GTATCC	1 cut(s)	370
BclI	TGATCA	1 cut(s)	400
BcnI	CCSGG	1 cut(s)	862
BcoDI	GTCTC	3 cut(s)	163, 1477, 2514
BcuI	ACTAGT	1 cut(s)	2657
BfmI	CTRYAG	2 cut(s)	167, 1173
BfrI	CTTAAG	1 cut(s)	1607
BfuI	GTATCC	1 cut(s)	370
BglI	GCCNNNNNGGC	1 cut(s)	106
BmcAI	AGTACT	2 cut(s)	2662, 2953
Bme1390I	CCNGG	2 cut(s)	221, 862
Bme18I	GGWCC	4 cut(s)	89, 935, 1184, 1409
BmeT110I	CYCGRG	3 cut(s)	94, 1881, 3110
BmgBI	CACGTC	1 cut(s)	2905
BmgT120I	GGNCC	8 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2242
BmiI	GGNNCC	7 cut(s)	91, 183, 496, 562, 993, 1532, 3100
BmrFI	CCNGG	2 cut(s)	221, 862
BmtI	GCTAGC	2 cut(s)	1649, 3132
BpiI	GAAGAC	3 cut(s)	702, 1370, 3045
BplI	GAGNNNNNCTC	2 cut(s)	143, 175
BpmI	CTGGAG	9 cut(s)	576, 692, 1595, 1988, 2294, 2315, 2609, 2765, 3155
Bpu10I	CCTNAGC	3 cut(s)	436, 1803, 1914
Bpu14I	TTCGAA	1 cut(s)	2573
BpuEI	CTTGAG	5 cut(s)	1082, 1823, 1887, 2391, 2845
BpuMI	CCSGG	1 cut(s)	862
BsaAI	YACGTR	1 cut(s)	542
BsaJI	CCNNGG	5 cut(s)	95, 220, 250, 1190, 2848
BsaWI	WCCGGW	2 cut(s)	86, 138
BsaXI	ACNNNNNCTCC	4 cut(s)	1259, 1289, 2288, 2318
Bsc4I	CCNNNNNNNGG	4 cut(s)	783, 1011, 1761, 2590
Bse118I	RCCGGY	1 cut(s)	86
Bse1I	ACTGG	7 cut(s)	348, 689, 938, 2277, 2592, 2775, 2958
Bse3DI	GCAATG	2 cut(s)	2460, 3130
BseAI	TCCGGA	1 cut(s)	138
BseBI	CCWGG	1 cut(s)	221
BseDI	CCNNGG	5 cut(s)	95, 220, 250, 1190, 2848
BseGI	GGATG	5 cut(s)	147, 352, 1627, 2183, 3165
BseLI	CCNNNNNNNGG	4 cut(s)	783, 1011, 1761, 2590
BseMI	GCAATG	2 cut(s)	2460, 3130
BseMII	CTCAG	7 cut(s)	1307, 1794, 1905, 1907, 2024, 2435, 2475
BseNI	ACTGG	7 cut(s)	348, 689, 938, 2277, 2592, 2775, 2958
BsePI	GCGCGC	1 cut(s)	2698
BseRI	GAGGAG	4 cut(s)	239, 1922, 1931, 2081
BseSI	GKGCMC	2 cut(s)	217, 325
BseX3I	CGGCCG	1 cut(s)	208
BseYI	CCCAGC	1 cut(s)	1499
BsgI	GTGCAG	1 cut(s)	324
Bsh1236I	CGCG	4 cut(s)	76, 200, 989, 2700
Bsh1285I	CGRYCG	3 cut(s)	80, 211, 505
BshFI	GGCC	9 cut(s)	100, 210, 787, 847, 904, 1462, 1665, 2243, 2682
BshNI	GGYRCC	3 cut(s)	991, 1530, 3098
BshTI	ACCGGT	1 cut(s)	86
BsiEI	CGRYCG	3 cut(s)	80, 211, 505
BsiHKAI	GWGCWC	4 cut(s)	325, 1215, 2401, 3111
BsiHKCI	CYCGRG	3 cut(s)	94, 1881, 3110
BsiSI	CCGG	3 cut(s)	87, 139, 861
BslFI	GGGAC	5 cut(s)	75, 364, 629, 722, 1828
BslI	CCNNNNNNNGG	4 cut(s)	783, 1011, 1761, 2590
BsmAI	GTCTC	3 cut(s)	163, 1477, 2514
BsmFI	GGGAC	5 cut(s)	75, 364, 629, 722, 1828
BsmI	GAATGC	3 cut(s)	773, 1112, 1261
BsnI	GGCC	9 cut(s)	100, 210, 787, 847, 904, 1462, 1665, 2243, 2682
BsoBI	CYCGRG	3 cut(s)	94, 1881, 3110
Bsp119I	TTCGAA	1 cut(s)	2573
Bsp1286I	GDGCHC	6 cut(s)	217, 325, 1215, 2401, 2407, 3111
Bsp13I	TCCGGA	1 cut(s)	138
Bsp1407I	TGTACA	1 cut(s)	296
Bsp143I	GATC	9 cut(s)	145, 400, 502, 805, 1096, 1145, 1810, 2917, 3148
BspANI	GGCC	9 cut(s)	100, 210, 787, 847, 904, 1462, 1665, 2243, 2682
BspCNI	CTCAG	7 cut(s)	1308, 1795, 1906, 1906, 2023, 2434, 2476
BspEI	TCCGGA	1 cut(s)	138
BspFNI	CGCG	4 cut(s)	76, 200, 989, 2700
BspLI	GGNNCC	7 cut(s)	91, 183, 496, 562, 993, 1532, 3100
BspMAI	CTGCAG	1 cut(s)	171
BspOI	GCTAGC	2 cut(s)	1649, 3132
BspPI	GGATC	2 cut(s)	153, 1091
BspQI	GCTCTTC	1 cut(s)	1036
BspT104I	TTCGAA	1 cut(s)	2573
BspT107I	GGYRCC	3 cut(s)	991, 1530, 3098
BspTI	CTTAAG	1 cut(s)	1607
BsrBI	CCGCTC	1 cut(s)	1536
BsrDI	GCAATG	2 cut(s)	2460, 3130
BsrFI	RCCGGY	1 cut(s)	86
BsrGI	TGTACA	1 cut(s)	296
BsrI	ACTGG	7 cut(s)	348, 689, 938, 2277, 2592, 2775, 2958
BssAI	RCCGGY	1 cut(s)	86
BssECI	CCNNGG	5 cut(s)	95, 220, 250, 1190, 2848
BssHII	GCGCGC	1 cut(s)	2698
BssMI	GATC	9 cut(s)	145, 400, 502, 805, 1096, 1145, 1810, 2917, 3148
BssSI	CACGAG	1 cut(s)	660
BssT1I	CCWWGG	1 cut(s)	1190
Bst2BI	CACGAG	1 cut(s)	660
Bst2UI	CCWGG	1 cut(s)	221
Bst4CI	ACNGT	6 cut(s)	960, 1283, 1694, 1984, 2665, 3058
Bst6I	CTCTTC	5 cut(s)	1012, 1036, 1442, 2009, 2590
BstAFI	CTTAAG	1 cut(s)	1607
BstAPI	GCANNNNNTGC	4 cut(s)	1331, 1961, 2417, 2708
BstAUI	TGTACA	1 cut(s)	296
BstBAI	YACGTR	1 cut(s)	542
BstBI	TTCGAA	1 cut(s)	2573
BstC8I	GCNNGC	7 cut(s)	217, 678, 1327, 1647, 1872, 2700, 3130
BstDSI	CCRYGG	1 cut(s)	2848
BstEII	GGTNACC	1 cut(s)	82
BstENI	CCTNNNNNAGG	1 cut(s)	2588
BstF5I	GGATG	5 cut(s)	147, 352, 1627, 2183, 3165
BstFNI	CGCG	4 cut(s)	76, 200, 989, 2700
BstHHI	GCGC	3 cut(s)	989, 2700, 2702
BstKTI	GATC	9 cut(s)	148, 403, 505, 808, 1099, 1148, 1813, 2920, 3151
BstMAI	GTCTC	3 cut(s)	163, 1477, 2514
BstMBI	GATC	9 cut(s)	145, 400, 502, 805, 1096, 1145, 1810, 2917, 3148
BstMCI	CGRYCG	3 cut(s)	80, 211, 505
BstNI	CCWGG	1 cut(s)	221
BstNSI	RCATGY	5 cut(s)	460, 1329, 1784, 2105, 2711
BstPI	GGTNACC	1 cut(s)	82
BstSCI	CCNGG	2 cut(s)	219, 860
BstSFI	CTRYAG	2 cut(s)	167, 1173
BstSLI	GKGCMC	2 cut(s)	217, 325
BstUI	CGCG	4 cut(s)	76, 200, 989, 2700
BstV2I	GAAGAC	3 cut(s)	702, 1370, 3045
BstX2I	RGATCY	1 cut(s)	1096
BstXI	CCANNNNNNTGG	2 cut(s)	479, 1419
BstYI	RGATCY	1 cut(s)	1096
BstZI	CGGCCG	1 cut(s)	208
BsuI	GTATCC	1 cut(s)	370
BsuRI	GGCC	9 cut(s)	100, 210, 787, 847, 904, 1462, 1665, 2243, 2682
BtgI	CCRYGG	1 cut(s)	2848
BtgZI	GCGATG	1 cut(s)	272
BtrI	CACGTC	1 cut(s)	2905
BtsCI	GGATG	5 cut(s)	147, 352, 1627, 2183, 3165
BtsI	GCAGTG	2 cut(s)	1858, 2117
BtsIMutI	CAGTG	4 cut(s)	341, 1279, 1858, 2117
Cac8I	GCNNGC	7 cut(s)	217, 678, 1327, 1647, 1872, 2700, 3130
CaiI	CAGNNNCTG	3 cut(s)	572, 1429, 2417
CfoI	GCGC	3 cut(s)	989, 2700, 2702
Cfr10I	RCCGGY	1 cut(s)	86
Cfr13I	GGNCC	8 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2242
CseI	GACGC	1 cut(s)	1076
Csp6I	GTAC	6 cut(s)	297, 415, 2305, 2661, 2952, 3154
CspAI	ACCGGT	1 cut(s)	86
CviQI	GTAC	6 cut(s)	297, 415, 2305, 2661, 2952, 3154
DpnI	GATC	9 cut(s)	147, 402, 504, 807, 1098, 1147, 1812, 2919, 3150
DpnII	GATC	9 cut(s)	145, 400, 502, 805, 1096, 1145, 1810, 2917, 3148
DraIII	CACNNNGTG	1 cut(s)	662
EaeI	YGGCCR	1 cut(s)	208
EagI	CGGCCG	1 cut(s)	208
Eam1104I	CTCTTC	5 cut(s)	1012, 1036, 1442, 2009, 2590
EarI	CTCTTC	5 cut(s)	1012, 1036, 1442, 2009, 2590
EciI	GGCGGA	2 cut(s)	279, 1223
Ecl136II	GAGCTC	1 cut(s)	1213
EclXI	CGGCCG	1 cut(s)	208
Eco130I	CCWWGG	1 cut(s)	1190
Eco147I	AGGCCT	2 cut(s)	847, 2682
Eco24I	GRGCYC	2 cut(s)	1215, 2407
Eco47I	GGWCC	4 cut(s)	89, 935, 1184, 1409
Eco52I	CGGCCG	1 cut(s)	208
Eco53kI	GAGCTC	1 cut(s)	1213
Eco57I	CTGAAG	1 cut(s)	402
Eco88I	CYCGRG	3 cut(s)	94, 1881, 3110
Eco91I	GGTNACC	1 cut(s)	82
EcoICRI	GAGCTC	1 cut(s)	1213
EcoNI	CCTNNNNNAGG	1 cut(s)	2588
EcoO65I	GGTNACC	1 cut(s)	82
EcoRI	GAATTC	3 cut(s)	851, 2039, 2544
EcoRII	CCWGG	1 cut(s)	219
EcoT14I	CCWWGG	1 cut(s)	1190
EcoT22I	ATGCAT	3 cut(s)	874, 1261, 3167
EcoT38I	GRGCYC	2 cut(s)	1215, 2407
ErhI	CCWWGG	1 cut(s)	1190
FalI	AAGNNNNNCTT	4 cut(s)	2827, 2859, 3129, 3161
FaqI	GGGAC	5 cut(s)	75, 364, 629, 722, 1828
FauI	CCCGC	2 cut(s)	224, 3023
FauNDI	CATATG	1 cut(s)	1048
FbaI	TGATCA	1 cut(s)	400
FblI	GTMKAC	2 cut(s)	114, 2868
FokI	GGATG	5 cut(s)	154, 359, 1634, 2170, 3152
FriOI	GRGCYC	2 cut(s)	1215, 2407
GlaI	GCGC	3 cut(s)	988, 2699, 2701
GsaI	CCCAGC	1 cut(s)	1503
GsuI	CTGGAG	9 cut(s)	576, 692, 1595, 1988, 2294, 2315, 2609, 2765, 3155
HaeIII	GGCC	9 cut(s)	100, 210, 787, 847, 904, 1462, 1665, 2243, 2682
HapII	CCGG	3 cut(s)	87, 139, 861
HgaI	GACGC	1 cut(s)	1076
HhaI	GCGC	3 cut(s)	989, 2700, 2702
Hin6I	GCGC	3 cut(s)	987, 2698, 2700
HinP1I	GCGC	3 cut(s)	987, 2698, 2700
HindIII	AAGCTT	2 cut(s)	549, 2192
HinfI	GANTC	8 cut(s)	29, 1052, 1577, 1736, 1751, 2309, 2774, 2891
HpaII	CCGG	3 cut(s)	87, 139, 861
HphI	GGTGA	6 cut(s)	27, 94, 766, 1715, 1871, 3046
Hpy166II	GTNNAC	5 cut(s)	115, 323, 1187, 2869, 3054
Hpy8I	GTNNAC	5 cut(s)	115, 323, 1187, 2869, 3054
Hpy99I	CGWCG	4 cut(s)	46, 52, 125, 200
HpyCH4III	ACNGT	6 cut(s)	960, 1283, 1694, 1984, 2665, 3058
HpyCH4IV	ACGT	4 cut(s)	413, 541, 711, 2904
HpySE526I	ACGT	4 cut(s)	413, 541, 711, 2904
HspAI	GCGC	3 cut(s)	987, 2698, 2700
Kpn2I	TCCGGA	1 cut(s)	138
Ksp22I	TGATCA	1 cut(s)	400
Kzo9I	GATC	9 cut(s)	145, 400, 502, 805, 1096, 1145, 1810, 2917, 3148
LguI	GCTCTTC	1 cut(s)	1036
LmnI	GCTCC	6 cut(s)	252, 673, 1210, 1218, 2275, 2296
MaeII	ACGT	4 cut(s)	413, 541, 711, 2904
MaeIII	GTNAC	4 cut(s)	82, 517, 1557, 2578
MalI	GATC	9 cut(s)	147, 402, 504, 807, 1098, 1147, 1812, 2919, 3150
MbiI	CCGCTC	1 cut(s)	1536
MboI	GATC	9 cut(s)	145, 400, 502, 805, 1096, 1145, 1810, 2917, 3148
MflI	RGATCY	1 cut(s)	1096
MhlI	GDGCHC	6 cut(s)	217, 325, 1215, 2401, 2407, 3111
MlyI	GAGTC	2 cut(s)	1586, 2885
MmeI	TCCRAC	2 cut(s)	813, 1410
Mph1103I	ATGCAT	3 cut(s)	874, 1261, 3167
MroI	TCCGGA	1 cut(s)	138
MroXI	GAANNNNTTC	1 cut(s)	11
MslI	CAYNNNNRTG	5 cut(s)	524, 540, 779, 1335, 2712
MspA1I	CMGCKG	1 cut(s)	1573
MspCI	CTTAAG	1 cut(s)	1607
MspI	CCGG	3 cut(s)	87, 139, 861
MspR9I	CCNGG	2 cut(s)	221, 862
Mva1269I	GAATGC	3 cut(s)	773, 1112, 1261
MvaI	CCWGG	1 cut(s)	221
MvnI	CGCG	4 cut(s)	76, 200, 989, 2700
NciI	CCSGG	1 cut(s)	862
NdeI	CATATG	1 cut(s)	1048
NdeII	GATC	9 cut(s)	145, 400, 502, 805, 1096, 1145, 1810, 2917, 3148
NheI	GCTAGC	2 cut(s)	1645, 3128
NlaIV	GGNNCC	7 cut(s)	91, 183, 496, 562, 993, 1532, 3100
NmuCI	GTSAC	2 cut(s)	82, 517
NsiI	ATGCAT	3 cut(s)	874, 1261, 3167
NspI	RCATGY	5 cut(s)	460, 1329, 1784, 2105, 2711
NspV	TTCGAA	1 cut(s)	2573
OliI	CACNNNNGTG	1 cut(s)	779
PaeI	GCATGC	1 cut(s)	1329
PaeR7I	CTCGAG	1 cut(s)	3110
PauI	GCGCGC	1 cut(s)	2698
PceI	AGGCCT	2 cut(s)	847, 2682
PciI	ACATGT	1 cut(s)	1780
PciSI	GCTCTTC	1 cut(s)	1036
PcsI	WCGNNNNNNNCGW	1 cut(s)	117
PctI	GAATGC	3 cut(s)	773, 1112, 1261
PdmI	GAANNNNTTC	1 cut(s)	11
PfeI	GAWTC	6 cut(s)	29, 1052, 1736, 1751, 2309, 2774
PflFI	GACNNNGTC	1 cut(s)	1475
PinAI	ACCGGT	1 cut(s)	86
Ple19I	CGATCG	1 cut(s)	505
PleI	GAGTC	2 cut(s)	1585, 2885
PpsI	GAGTC	2 cut(s)	1585, 2885
Ppu21I	YACGTR	1 cut(s)	542
PscI	ACATGT	1 cut(s)	1780
Psp124BI	GAGCTC	1 cut(s)	1215
Psp6I	CCWGG	1 cut(s)	219
PspEI	GGTNACC	1 cut(s)	82
PspFI	CCCAGC	1 cut(s)	1499
PspGI	CCWGG	1 cut(s)	219
PspN4I	GGNNCC	7 cut(s)	91, 183, 496, 562, 993, 1532, 3100
PspPI	GGNCC	8 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2242
PspXI	VCTCGAGB	1 cut(s)	3110
PsrI	GAACNNNNNNTAC	2 cut(s)	1678, 1710
PstI	CTGCAG	1 cut(s)	171
PstNI	CAGNNNCTG	3 cut(s)	572, 1429, 2417
PsuI	RGATCY	1 cut(s)	1096
PsyI	GACNNNGTC	1 cut(s)	1475
PteI	GCGCGC	1 cut(s)	2698
PvuI	CGATCG	1 cut(s)	505
PvuII	CAGCTG	1 cut(s)	1573
RsaI	GTAC	6 cut(s)	298, 416, 2306, 2662, 2953, 3155
RsaNI	GTAC	6 cut(s)	297, 415, 2305, 2661, 2952, 3154
RseI	CAYNNNNRTG	5 cut(s)	524, 540, 779, 1335, 2712
SacI	GAGCTC	1 cut(s)	1215
SapI	GCTCTTC	1 cut(s)	1036
Sau3AI	GATC	9 cut(s)	145, 400, 502, 805, 1096, 1145, 1810, 2917, 3148
Sau96I	GGNCC	8 cut(s)	89, 903, 935, 1184, 1409, 1460, 1663, 2242
ScaI	AGTACT	2 cut(s)	2662, 2953
SchI	GAGTC	2 cut(s)	1586, 2885
ScrFI	CCNGG	2 cut(s)	221, 862
SduI	GDGCHC	6 cut(s)	217, 325, 1215, 2401, 2407, 3111
SfcI	CTRYAG	2 cut(s)	167, 1173
Sfr274I	CTCGAG	1 cut(s)	3110
SfuI	TTCGAA	1 cut(s)	2573
SinI	GGWCC	4 cut(s)	89, 935, 1184, 1409
SlaI	CTCGAG	1 cut(s)	3110
SmiMI	CAYNNNNRTG	5 cut(s)	524, 540, 779, 1335, 2712
SmlI	CTYRAG	7 cut(s)	1061, 1607, 1838, 1902, 2406, 2860, 3110
SmoI	CTYRAG	7 cut(s)	1061, 1607, 1838, 1902, 2406, 2860, 3110
SpeI	ACTAGT	1 cut(s)	2657
SphI	GCATGC	1 cut(s)	1329
SseBI	AGGCCT	2 cut(s)	847, 2682
SspI	AATATT	1 cut(s)	1348
SstI	GAGCTC	1 cut(s)	1215
StuI	AGGCCT	2 cut(s)	847, 2682
StyD4I	CCNGG	2 cut(s)	219, 860
StyI	CCWWGG	1 cut(s)	1190
TaaI	ACNGT	6 cut(s)	960, 1283, 1694, 1984, 2665, 3058
TaiI	ACGT	4 cut(s)	416, 544, 714, 2907
TaqI	TCGA	7 cut(s)	501, 693, 745, 1592, 2153, 2573, 3111
TatI	WGTACW	3 cut(s)	296, 2660, 2951
TauI	GCSGC	4 cut(s)	210, 787, 1536, 1668
TfiI	GAWTC	6 cut(s)	29, 1052, 1736, 1751, 2309, 2774
TscAI	CASTG	4 cut(s)	348, 1286, 1858, 2117
TseFI	GTSAC	2 cut(s)	82, 517
Tsp45I	GTSAC	2 cut(s)	82, 517
TspGWI	ACGGA	1 cut(s)	422
TspRI	CASTG	4 cut(s)	348, 1286, 1858, 2117
Tth111I	GACNNNGTC	1 cut(s)	1475
Vha464I	CTTAAG	1 cut(s)	1607
VneI	GTGCAC	1 cut(s)	321
VpaK11BI	GGWCC	4 cut(s)	89, 935, 1184, 1409
XagI	CCTNNNNNAGG	1 cut(s)	2588
XapI	RAATTY	6 cut(s)	8, 628, 825, 851, 2039, 2544
XbaI	TCTAGA	1 cut(s)	2945
XceI	RCATGY	5 cut(s)	460, 1329, 1784, 2105, 2711
XcmI	CCANNNNNNNNNTGG	2 cut(s)	600, 1300
XhoI	CTCGAG	1 cut(s)	3110
XmiI	GTMKAC	2 cut(s)	114, 2868
XmnI	GAANNNNTTC	1 cut(s)	11
ZrmI	AGTACT	2 cut(s)	2662, 2953
Zsp2I	ATGCAT	3 cut(s)	874, 1261, 3167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.