Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0007375

Physical Location & Seq

Genomic Coordinates Forward (+)

88244190 .. 88251308

Gene Structure 7119 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00066750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2988 bp

ATGTTTCGCATGTTCAGAGGAGGCAACGCCTCCGATGCCTCGCCGGAATCTTCCTCCTCTCCGTCGTCGTCGTCCTCGCCTTCGCCGCCGCAGCCGTCTCGGCCGGTGACCGGGCCGGCGAGGCCTATTCGACTCGTCTACGCCGACGAGAAAGGGAGGTTCCGGATGGATCCGGAAGCCGTCGCGGTGTTGCAGCTCGTTAAGGAGCCGATTGGTGTCGTCTCCGTCTGCGGTCGCGCTCGCCAGGGCAAGAGCTATATTCTCAACCAGGGAACGTACAGCACACAAATATTTTCCTTGGCTGTTCTCTTATCCAGCATGTTCATTTATAATCAGGTTAGGAGACTTCTGCTGATGGGTGGTATAGATGAAGCTTCACTTGATCGTCTTGCTCTTGTCACCCAAGTGACCAAACATATCCGTGTTAAAGCCTCTGGAGGAAGAACCACAGCCTCTGAAATCGGGCAGTTCTCCCCAGTTTTTGTCTGGCTTCTAAGGGACTTCTATCTCGAATTAGTAGATAATGGTAGAAAGATAACACCCCGTGAGTATCTAGAGACTGCTTTGAGGCCTTTTGAAGGAAAACAAGACGTAGCTGCCAAAAATGAGATACGGGACTCCATTAGAGCTCTATTTCCAGATAGAGAATGCTTCACCCTTTTGCGGCCTGTGGACAAGGAAGAGGAGCTCCGGAGACTCGATCAAATTCAGTTGAACAAGTTAAGGCCTGAGTTTAGGTCAGGGCTGGATGCTTTGACAAGTTTTGTTTTTAAACGAACGAGGCCCAAGCAAGTTGGGGCCACTATGATGACAGGGCCAGTTCTAGTTGGTATCCTTCAGTCCTATCTTGATGCTCTTAATAATGGTGCAGTGCCTACCATCTCATCTTCATGGCAGAGTGTCGAAGAAGCAGAGTGCCGAAGAGCATATGATTCTGCTGTTGATGCTTATAGGTCTGCTTTTGACCGTTCAAAGTTGCCTGAAGAAGCTGCACTGAGGGAGGCTCATGAAGAGGCAGTCCAAAAATCACTGGCTGCTTTTAATGATTGTGCTGTAGGGGGTGGTCCAACAAGGAAAAAATATGAAGGGAATCTTCACAAACAGCTCAAAAAGGAATTTGAAGATTATAAGAAGAATGCATATATGGAGGCAGAGTTGCAATGTGTAAATGCTATACAAAGGATGGAAGGGAAGCTGAGGAGCGCTTGCCATGCTAGTGATGCTAATATTGATAATGTTCTGAAGGTTCTTGGTGACCTTATGTCCGAGTATGAAAAGGCAAGTAGGGGTCCACTAAAATGGCAGCAACTGGCTTTCTTTTTAAGAAAGAGTTTGGAAGGTCCAATTCTTGATCTCATCATGAGGCAAATCAACAAAGTTGAATCACAGAATGGCACCTTGAGGTTGAGATCCCGTGCCATGGAAGATGAGTTGGGTTTACTAAAAAAGCGGGTAGAGGAAAGCAAGCAGTCTAAGATGGAGTATGTCAAACGCTACGAGGACGCTCTCAATGACAATAACAAGCTAAAGGATGAATACAATGTCCGTATTAACAACTTGCAAGGAAATAATACTTCTCTGCGAGATAAGTGTGCTAGCTTGCGTAAGAGTTTGGATTCTGTAAAAGCAGAAGCAGGAGAATGGCAAAGAAAATATGAGCATGTTCTCTCCAAGCAGAAGGCTGAGGAAGATCAAGCTGGTTCCGAAATCGCTGTTCTCAAGTCTCGATGCAGTGCTGGCGAAGCAAGGCTGGCTGCTGCAAAGGAACAAGCTCAGTCTGCCCAAGAGGAGGCAGAGGACTGGAAACGCAAATATGACATTGCTTTTAGAGAGGCAAAAGCTGCTCTAGAGAAGGCAGCAATTGTCCAAGAACGTTCCAGTAAGGAAACACAAAGGAGAGAAGATGCTTTGAGGGAAGAATTCACAAGTAGTTTGGCTGCGAAGGCTGCCAGGTCCAAAATTGATAGTTATGATGCTGAAATATCATCAGGAAAGCTTGAAATCAAGGAGCTGAGTAGAAAATTAGAAGCAGCAAATGACAAGGCCAATTCATTTGAAAGAGAAAAGAAGATACTGGAGCAGGAGAAGATCCATCTAAAGCAGACGTATGAAACTGAGATCAAAAGATTGGATGAAGTCCAAGAAAGGTGTAAAATTGCTGAAAAAGAAGCCACTAGAGCAACTGATATTGCTGATAAAGCACGAGCTCAAGCAGACATAGCTCAAAAGGAAAAGGGGGAGATGCAGAGGCTGGCAATAGAAAGGTTGGCTCAAATTGAGAGGGCTGAGAGGCATATTGAAAGTCTACAGAGGGAGAAAAGAGACTTGGAAGTTGAGTTGGAAGGAATCCGTGCATCAGAAAGGGGAGCTCATCACAAAATTTCTCTACTGGAGGCAAGAGTTGAGGAAAGGGAGAAAGAAATAGAATCATTATTGGAGTCAAACAATGAGCAGAGAACTAGCACAGTTCAAGTCCTTCAAGGTCTTCTGGACAGTGAACGTGCAGCACATGCAGACGCAAACAATAGGGCAGAAGCCCTCTCTCATCAGCTACAATCTGCACAAGCAAAACTCGATAAACTCCAGCAAGAATTGACTTCTGTTCGCCTGAATGAAACTGCATTGGATAGTAAGCTTAGGACTGCTTCACATGGGAAGCGTTCAAGGGTAGATGACTATGACATGGACGTGGACTCTGTTCAAGATGGGGAATTGAGTGACCGGATCTTAAGAGTAAATAAGAAGTCTAGGAGTACTACTAGCCCTCTGAAGCATGCACAAACAGAAGATGGTGGTTCAGTATTCCGGGGAGATGAGGATAGCCGCAGTCAACAAACCAATCCAGAAGATTACACAAAGTTCACAGTCCAGAAACTGAAGCAAGAGCTAACAAAGCATAATTTCGGTGCTGAGCTGCTGCAGTTAAGGAACCCCAACAAAAGGGAGATCCTCACACTATATGAGAAGTGCATTCTTCAAAAGTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0008104 GO:0008150 GO:0009987 GO:0010033 GO:0012505 GO:0015629 GO:0017076 GO:0019001 GO:0019221 GO:0023052 GO:0031974 GO:0031981 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0033036 GO:0033365 GO:0034097 GO:0034340 GO:0034341 GO:0034504 GO:0034612 GO:0034613 GO:0035639 GO:0036094 GO:0042221 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046983 GO:0048471 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051641 GO:0051716 GO:0060333 GO:0060337 GO:0065007 GO:0070013 GO:0070555 GO:0070727 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071347 GO:0071356 GO:0071357 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04621 map04621

Pfam Domains

Protein Families

GBP GBP_C

Protein Analysis

995

Amino Acids

112.66

Weight (kDa)

8.54

Isoelectric Point (pI)

56.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GBP	PF02263	94 - 261	2.5e-33	Guanylate-binding protein, N-terminal domain
GBP_C	PF02841	266 - 569	4.7e-31	Guanylate-binding protein, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46070
fragaria_vesca	FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471
malus_domestica	MD05G1309600.v1.1 MD10G1288200.v1.1
prunus_persica	Prupe.4G053400_v2.0.a1
pyrus_communis	pycom05g28600 pycom10g24130 pycom10g24140
rosa_chinensis	RchiOBHm_Chr5g0009341
rosa_laevigata	RLG00000031653
rosa_multiflora	Rmu_sc0002711.1_g000023 Rmu_sc0009480.1_g000002
rosa_roxburghii	Rroxscaffold_1G00066750 Rroxscaffold_1G00067450
rosa_rugosa	Rorug04G0441800
rosa_samantha	Rh5AG072700 Rh5BG069000 Rh5CG081600 Rh5DG069300
rosa_wichuraiana	Rw5G006790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	330, 1128
AccB1I	GGYRCC	1 cut(s)	1394
AccI	GTMKAC	2 cut(s)	138, 2305
AccII	CGCG	2 cut(s)	185, 237
AccIII	TCCGGA	3 cut(s)	162, 172, 690
AciI	CCGC	7 cut(s)	86, 89, 185, 231, 664, 1450, 2824
AclI	AACGTT	1 cut(s)	1873
AclWI	GGATC	6 cut(s)	164, 177, 1404, 2083, 2732, 2941
AcoI	YGGCCR	1 cut(s)	101
AcsI	RAATTY	4 cut(s)	705, 1115, 1919, 2379
AcuI	CTGAAG	5 cut(s)	821, 1002, 1262, 2789, 2897
AdeI	CACNNNGTG	1 cut(s)	545
AfaI	GTAC	2 cut(s)	278, 2755
AfeI	AGCGCT	1 cut(s)	1204
AfiI	CCNNNNNNNGG	6 cut(s)	110, 578, 663, 1420, 1789, 2940
AflII	CTTAAG	1 cut(s)	2728
AhdI	GACNNNNNGTC	1 cut(s)	1261
AjiI	CACGTC	1 cut(s)	2689
AjnI	CCWGG	3 cut(s)	243, 267, 1949
AjuI	GAANNNNNNNTTGG	2 cut(s)	2309, 2341
AleI	CACNNNNGTG	1 cut(s)	404
Alw21I	GWGCWC	4 cut(s)	631, 690, 2209, 2371
Alw26I	GTCTC	7 cut(s)	102, 226, 337, 551, 688, 1728, 2316
AlwI	GGATC	6 cut(s)	164, 177, 1404, 2083, 2732, 2941
AlwNI	CAGNNNCTG	4 cut(s)	455, 1309, 2251, 2875
Aor13HI	TCCGGA	3 cut(s)	162, 172, 690
Aor51HI	AGCGCT	1 cut(s)	1204
ApoI	RAATTY	4 cut(s)	705, 1115, 1919, 2379
AspLEI	GCGC	2 cut(s)	239, 1205
AspS9I	GGNCC	8 cut(s)	113, 783, 798, 815, 1064, 1289, 1340, 1953
AsuC2I	CCSGG	2 cut(s)	112, 2807
AsuHPI	GGTGA	4 cut(s)	118, 391, 646, 1265
AsuNHI	GCTAGC	1 cut(s)	1595
AvaII	GGWCC	4 cut(s)	1064, 1289, 1340, 1953
BamHI	GGATCC	1 cut(s)	169
BanI	GGYRCC	1 cut(s)	1394
BanII	GRGCYC	4 cut(s)	631, 690, 2209, 2371
BauI	CACGAG	1 cut(s)	2202
BbsI	GAAGAC	1 cut(s)	2477
Bbv12I	GWGCWC	4 cut(s)	631, 690, 2209, 2371
BbvCI	CCTCAGC	2 cut(s)	1196, 1683
BccI	CCATC	8 cut(s)	160, 349, 887, 1177, 1471, 2100, 2699, 2783
BceAI	ACGGC	2 cut(s)	79, 164
BcgI	CGANNNNNNTGC	2 cut(s)	80, 114
BciT130I	CCWGG	3 cut(s)	245, 269, 1951
BciVI	GTATCC	1 cut(s)	842
BcnI	CCSGG	2 cut(s)	112, 2807
BcoDI	GTCTC	7 cut(s)	102, 226, 337, 551, 688, 1728, 2316
BfaI	CTAG	9 cut(s)	554, 824, 1215, 1596, 1847, 2175, 2460, 2748, 2760
BfmI	CTRYAG	3 cut(s)	1053, 2306, 2918
BfoI	RGCGCY	1 cut(s)	1206
BfrI	CTTAAG	1 cut(s)	2728
BfuI	GTATCC	1 cut(s)	842
BglI	GCCNNNNNGGC	2 cut(s)	100, 121
BlpI	GCTNAGC	1 cut(s)	2910
BmcAI	AGTACT	1 cut(s)	2755
Bme1390I	CCNGG	5 cut(s)	112, 245, 269, 1951, 2807
Bme18I	GGWCC	4 cut(s)	1064, 1289, 1340, 1953
BmeRI	GACNNNNNGTC	1 cut(s)	1261
BmgBI	CACGTC	1 cut(s)	2689
BmgT120I	GGNCC	8 cut(s)	113, 783, 798, 815, 1064, 1289, 1340, 1953
BmiI	GGNNCC	8 cut(s)	161, 171, 207, 799, 1290, 1396, 1702, 2930
BmrFI	CCNGG	5 cut(s)	112, 245, 269, 1951, 2807
BmrI	ACTGGG	1 cut(s)	470
BmtI	GCTAGC	1 cut(s)	1599
BmuI	ACTGGG	1 cut(s)	470
BpiI	GAAGAC	1 cut(s)	2477
BplI	GAGNNNNNCTC	2 cut(s)	988, 1020
BpmI	CTGGAG	4 cut(s)	456, 2096, 2411, 2567
Bpu10I	CCTNAGC	3 cut(s)	1196, 1683, 2636
Bpu1102I	GCTNAGC	1 cut(s)	2910
BpuEI	CTTGAG	3 cut(s)	1420, 1703, 2193
BpuMI	CCSGG	2 cut(s)	112, 2807
BsaJI	CCNNGG	5 cut(s)	244, 268, 297, 1419, 2806
BsaWI	WCCGGW	4 cut(s)	162, 172, 690, 2721
BsaXI	ACNNNNNCTCC	2 cut(s)	1139, 1169
Bsc4I	CCNNNNNNNGG	6 cut(s)	110, 578, 663, 1420, 1789, 2940
Bse118I	RCCGGY	2 cut(s)	103, 115
Bse1I	ACTGG	8 cut(s)	476, 818, 1035, 1314, 1805, 1878, 2079, 2394
Bse3DI	GCAATG	2 cut(s)	1166, 1818
BseAI	TCCGGA	3 cut(s)	162, 172, 690
BseBI	CCWGG	3 cut(s)	245, 269, 1951
BseDI	CCNNGG	5 cut(s)	244, 268, 297, 1419, 2806
BseGI	GGATG	5 cut(s)	171, 754, 1188, 1537, 2137
BseLI	CCNNNNNNNGG	6 cut(s)	110, 578, 663, 1420, 1789, 2940
BseMI	GCAATG	2 cut(s)	1166, 1818
BseMII	CTCAG	9 cut(s)	720, 986, 1187, 1674, 1787, 2003, 2106, 2277, 2901
BseNI	ACTGG	8 cut(s)	476, 818, 1035, 1314, 1805, 1878, 2079, 2394
BseRI	GAGGAG	5 cut(s)	33, 46, 698, 1213, 1802
BseX3I	CGGCCG	1 cut(s)	101
BsgI	GTGCAG	4 cut(s)	888, 975, 2523, 2544
Bsh1236I	CGCG	2 cut(s)	185, 237
Bsh1285I	CGRYCG	2 cut(s)	104, 235
BshNI	GGYRCC	1 cut(s)	1394
BsiEI	CGRYCG	2 cut(s)	104, 235
BsiHKAI	GWGCWC	4 cut(s)	631, 690, 2209, 2371
BsiSI	CCGG	9 cut(s)	44, 104, 111, 116, 163, 173, 691, 2722, 2806
BslFI	GGGAC	2 cut(s)	512, 629
BslI	CCNNNNNNNGG	6 cut(s)	110, 578, 663, 1420, 1789, 2940
BsmAI	GTCTC	7 cut(s)	102, 226, 337, 551, 688, 1728, 2316
BsmBI	CGTCTC	2 cut(s)	102, 226
BsmFI	GGGAC	2 cut(s)	512, 629
BsmI	GAATGC	3 cut(s)	653, 1141, 2970
Bsp1286I	GDGCHC	4 cut(s)	631, 690, 2209, 2371
Bsp13I	TCCGGA	3 cut(s)	162, 172, 690
Bsp1720I	GCTNAGC	1 cut(s)	2910
Bsp19I	CCATGG	1 cut(s)	1419
BspACI	CCGC	7 cut(s)	86, 89, 185, 231, 664, 1450, 2824
BspCNI	CTCAG	9 cut(s)	721, 987, 1188, 1675, 1786, 2004, 2107, 2278, 2902
BspEI	TCCGGA	3 cut(s)	162, 172, 690
BspFNI	CGCG	2 cut(s)	185, 237
BspHI	TCATGA	2 cut(s)	1006, 1359
BspLI	GGNNCC	8 cut(s)	161, 171, 207, 799, 1290, 1396, 1702, 2930
BspMAI	CTGCAG	1 cut(s)	2922
BspOI	GCTAGC	1 cut(s)	1599
BspPI	GGATC	6 cut(s)	164, 177, 1404, 2083, 2732, 2941
BspQI	GCTCTTC	1 cut(s)	916
BspT107I	GGYRCC	1 cut(s)	1394
BspTI	CTTAAG	1 cut(s)	2728
BsrDI	GCAATG	2 cut(s)	1166, 1818
BsrFI	RCCGGY	2 cut(s)	103, 115
BsrI	ACTGG	8 cut(s)	476, 818, 1035, 1314, 1805, 1878, 2079, 2394
BssAI	RCCGGY	2 cut(s)	103, 115
BssECI	CCNNGG	5 cut(s)	244, 268, 297, 1419, 2806
BssSI	CACGAG	1 cut(s)	2202
BssT1I	CCWWGG	2 cut(s)	297, 1419
Bst2BI	CACGAG	1 cut(s)	2202
Bst2UI	CCWGG	3 cut(s)	245, 269, 1951
Bst4CI	ACNGT	4 cut(s)	968, 2467, 2495, 2866
Bst6I	CTCTTC	3 cut(s)	675, 916, 1005
BstAFI	CTTAAG	1 cut(s)	2728
BstAPI	GCANNNNNTGC	2 cut(s)	1841, 2510
BstDSI	CCRYGG	1 cut(s)	1419
BstEII	GGTNACC	2 cut(s)	106, 1253
BstENI	CCTNNNNNAGG	1 cut(s)	576
BstF5I	GGATG	5 cut(s)	171, 754, 1188, 1537, 2137
BstFNI	CGCG	2 cut(s)	185, 237
BstH2I	RGCGCY	1 cut(s)	1206
BstHHI	GCGC	2 cut(s)	239, 1205
BstMAI	GTCTC	7 cut(s)	102, 226, 337, 551, 688, 1728, 2316
BstMCI	CGRYCG	2 cut(s)	104, 235
BstNI	CCWGG	3 cut(s)	245, 269, 1951
BstNSI	RCATGY	5 cut(s)	13, 322, 1664, 2513, 2777
BstPI	GGTNACC	2 cut(s)	106, 1253
BstSCI	CCNGG	5 cut(s)	110, 243, 267, 1949, 2805
BstSFI	CTRYAG	3 cut(s)	1053, 2306, 2918
BstUI	CGCG	2 cut(s)	185, 237
BstV2I	GAAGAC	1 cut(s)	2477
BstX2I	RGATCY	5 cut(s)	169, 1409, 2088, 2724, 2946
BstXI	CCANNNNNNTGG	1 cut(s)	1299
BstYI	RGATCY	5 cut(s)	169, 1409, 2088, 2724, 2946
BstZI	CGGCCG	1 cut(s)	101
BsuI	GTATCC	1 cut(s)	842
BtgI	CCRYGG	1 cut(s)	1419
BtrI	CACGTC	1 cut(s)	2689
BtsCI	GGATG	5 cut(s)	171, 754, 1188, 1537, 2137
BtsI	GCAGTG	2 cut(s)	876, 1738
BtsIMutI	CAGTG	5 cut(s)	876, 992, 1028, 1738, 2500
CaiI	CAGNNNCTG	4 cut(s)	455, 1309, 2251, 2875
CciI	TCATGA	2 cut(s)	1006, 1359
CfoI	GCGC	2 cut(s)	239, 1205
Cfr10I	RCCGGY	2 cut(s)	103, 115
Cfr13I	GGNCC	8 cut(s)	113, 783, 798, 815, 1064, 1289, 1340, 1953
CseI	GACGC	2 cut(s)	1511, 2525
Csp6I	GTAC	2 cut(s)	277, 2754
CviQI	GTAC	2 cut(s)	277, 2754
DraI	TTTAAA	1 cut(s)	772
DraIII	CACNNNGTG	1 cut(s)	545
DriI	GACNNNNNGTC	1 cut(s)	1261
EaeI	YGGCCR	1 cut(s)	101
EagI	CGGCCG	1 cut(s)	101
Eam1104I	CTCTTC	3 cut(s)	675, 916, 1005
Eam1105I	GACNNNNNGTC	1 cut(s)	1261
EarI	CTCTTC	3 cut(s)	675, 916, 1005
Ecl136II	GAGCTC	4 cut(s)	629, 688, 2207, 2369
EclXI	CGGCCG	1 cut(s)	101
Eco130I	CCWWGG	2 cut(s)	297, 1419
Eco147I	AGGCCT	3 cut(s)	124, 571, 727
Eco24I	GRGCYC	4 cut(s)	631, 690, 2209, 2371
Eco47I	GGWCC	4 cut(s)	1064, 1289, 1340, 1953
Eco47III	AGCGCT	1 cut(s)	1204
Eco52I	CGGCCG	1 cut(s)	101
Eco53kI	GAGCTC	4 cut(s)	629, 688, 2207, 2369
Eco57I	CTGAAG	5 cut(s)	821, 1002, 1262, 2789, 2897
Eco91I	GGTNACC	2 cut(s)	106, 1253
EcoICRI	GAGCTC	4 cut(s)	629, 688, 2207, 2369
EcoNI	CCTNNNNNAGG	1 cut(s)	576
EcoO65I	GGTNACC	2 cut(s)	106, 1253
EcoRI	GAATTC	1 cut(s)	1919
EcoRII	CCWGG	3 cut(s)	243, 267, 1949
EcoT14I	CCWWGG	2 cut(s)	297, 1419
EcoT22I	ATGCAT	1 cut(s)	1141
EcoT38I	GRGCYC	4 cut(s)	631, 690, 2209, 2371
ErhI	CCWWGG	2 cut(s)	297, 1419
Esp3I	CGTCTC	2 cut(s)	102, 226
FalI	AAGNNNNNCTT	4 cut(s)	363, 395, 1077, 1109
FaqI	GGGAC	2 cut(s)	512, 629
FauI	CCCGC	1 cut(s)	1443
FauNDI	CATATG	1 cut(s)	928
FblI	GTMKAC	2 cut(s)	138, 2305
FokI	GGATG	5 cut(s)	178, 761, 1195, 1544, 2144
FriOI	GRGCYC	4 cut(s)	631, 690, 2209, 2371
FspBI	CTAG	9 cut(s)	554, 824, 1215, 1596, 1847, 2175, 2460, 2748, 2760
GlaI	GCGC	2 cut(s)	238, 1204
GsuI	CTGGAG	4 cut(s)	456, 2096, 2411, 2567
HaeII	RGCGCY	1 cut(s)	1206
HapII	CCGG	9 cut(s)	44, 104, 111, 116, 163, 173, 691, 2722, 2806
HgaI	GACGC	2 cut(s)	1511, 2525
HhaI	GCGC	2 cut(s)	239, 1205
Hin6I	GCGC	2 cut(s)	237, 1203
HinP1I	GCGC	2 cut(s)	237, 1203
HincII	GTYRAC	1 cut(s)	2831
HindII	GTYRAC	1 cut(s)	2831
HindIII	AAGCTT	3 cut(s)	372, 1994, 2633
HpaII	CCGG	9 cut(s)	44, 104, 111, 116, 163, 173, 691, 2722, 2806
HphI	GGTGA	4 cut(s)	118, 391, 646, 1265
Hpy166II	GTNNAC	9 cut(s)	139, 673, 1292, 1439, 2306, 2498, 2692, 2831, 2862
Hpy188I	TCNGA	8 cut(s)	17, 34, 457, 1242, 1267, 1705, 2359, 2769
Hpy8I	GTNNAC	9 cut(s)	139, 673, 1292, 1439, 2306, 2498, 2692, 2831, 2862
Hpy99I	CGWCG	5 cut(s)	67, 70, 73, 149, 185
HpyCH4III	ACNGT	4 cut(s)	968, 2467, 2495, 2866
HpyCH4IV	ACGT	6 cut(s)	275, 591, 1873, 2105, 2500, 2688
HpySE526I	ACGT	6 cut(s)	275, 591, 1873, 2105, 2500, 2688
HspAI	GCGC	2 cut(s)	237, 1203
Kpn2I	TCCGGA	3 cut(s)	162, 172, 690
KroI	GCCGGC	1 cut(s)	115
KroNI	GCCGGC	1 cut(s)	117
LguI	GCTCTTC	1 cut(s)	916
LmnI	GCTCC	7 cut(s)	205, 685, 693, 1200, 2008, 2077, 2366
MaeI	CTAG	9 cut(s)	554, 824, 1215, 1596, 1847, 2175, 2460, 2748, 2760
MaeII	ACGT	6 cut(s)	275, 591, 1873, 2105, 2500, 2688
MaeIII	GTNAC	5 cut(s)	106, 397, 406, 1253, 2717
MfeI	CAATTG	1 cut(s)	1860
MflI	RGATCY	5 cut(s)	169, 1409, 2088, 2724, 2946
MhlI	GDGCHC	4 cut(s)	631, 690, 2209, 2371
MlyI	GAGTC	5 cut(s)	126, 611, 690, 2447, 2687
MmeI	TCCRAC	2 cut(s)	1091, 2319
Mph1103I	ATGCAT	1 cut(s)	1141
MroI	TCCGGA	3 cut(s)	162, 172, 690
MroNI	GCCGGC	1 cut(s)	115
MslI	CAYNNNNRTG	7 cut(s)	404, 420, 806, 889, 1215, 1297, 2687
MspCI	CTTAAG	1 cut(s)	2728
MspI	CCGG	9 cut(s)	44, 104, 111, 116, 163, 173, 691, 2722, 2806
MspR9I	CCNGG	5 cut(s)	112, 245, 269, 1951, 2807
MunI	CAATTG	1 cut(s)	1860
Mva1269I	GAATGC	3 cut(s)	653, 1141, 2970
MvaI	CCWGG	3 cut(s)	245, 269, 1951
MvnI	CGCG	2 cut(s)	185, 237
NaeI	GCCGGC	1 cut(s)	117
NciI	CCSGG	2 cut(s)	112, 2807
NcoI	CCATGG	1 cut(s)	1419
NdeI	CATATG	1 cut(s)	928
NgoMIV	GCCGGC	1 cut(s)	115
NheI	GCTAGC	1 cut(s)	1595
NlaIV	GGNNCC	8 cut(s)	161, 171, 207, 799, 1290, 1396, 1702, 2930
NmeAIII	GCCGAG	1 cut(s)	79
NmuCI	GTSAC	5 cut(s)	106, 397, 406, 1253, 2717
NsiI	ATGCAT	1 cut(s)	1141
NspI	RCATGY	5 cut(s)	13, 322, 1664, 2513, 2777
OliI	CACNNNNGTG	1 cut(s)	404
PaeI	GCATGC	1 cut(s)	2777
PagI	TCATGA	2 cut(s)	1006, 1359
PceI	AGGCCT	3 cut(s)	124, 571, 727
PciSI	GCTCTTC	1 cut(s)	916
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PctI	GAATGC	3 cut(s)	653, 1141, 2970
PdiI	GCCGGC	1 cut(s)	117
PfeI	GAWTC	7 cut(s)	47, 932, 1090, 1382, 1616, 2346, 2426
PleI	GAGTC	5 cut(s)	126, 611, 690, 2446, 2687
PpsI	GAGTC	5 cut(s)	126, 611, 690, 2446, 2687
PsiI	TTATAA	2 cut(s)	330, 1128
Psp124BI	GAGCTC	4 cut(s)	631, 690, 2209, 2371
Psp1406I	AACGTT	1 cut(s)	1873
Psp6I	CCWGG	3 cut(s)	243, 267, 1949
PspEI	GGTNACC	2 cut(s)	106, 1253
PspGI	CCWGG	3 cut(s)	243, 267, 1949
PspN4I	GGNNCC	8 cut(s)	161, 171, 207, 799, 1290, 1396, 1702, 2930
PspPI	GGNCC	8 cut(s)	113, 783, 798, 815, 1064, 1289, 1340, 1953
PstI	CTGCAG	1 cut(s)	2922
PstNI	CAGNNNCTG	4 cut(s)	455, 1309, 2251, 2875
PsuI	RGATCY	5 cut(s)	169, 1409, 2088, 2724, 2946
RsaI	GTAC	2 cut(s)	278, 2755
RsaNI	GTAC	2 cut(s)	277, 2754
RseI	CAYNNNNRTG	7 cut(s)	404, 420, 806, 889, 1215, 1297, 2687
SacI	GAGCTC	4 cut(s)	631, 690, 2209, 2371
SapI	GCTCTTC	1 cut(s)	916
Sau96I	GGNCC	8 cut(s)	113, 783, 798, 815, 1064, 1289, 1340, 1953
ScaI	AGTACT	1 cut(s)	2755
SchI	GAGTC	5 cut(s)	126, 611, 690, 2447, 2687
ScrFI	CCNGG	5 cut(s)	112, 245, 269, 1951, 2807
SduI	GDGCHC	4 cut(s)	631, 690, 2209, 2371
SfcI	CTRYAG	3 cut(s)	1053, 2306, 2918
SfiI	GGCCNNNNNGGCC	1 cut(s)	121
SinI	GGWCC	4 cut(s)	1064, 1289, 1340, 1953
SmiMI	CAYNNNNRTG	7 cut(s)	404, 420, 806, 889, 1215, 1297, 2687
SmlI	CTYRAG	4 cut(s)	1399, 1718, 2208, 2728
SmoI	CTYRAG	4 cut(s)	1399, 1718, 2208, 2728
SphI	GCATGC	1 cut(s)	2777
SseBI	AGGCCT	3 cut(s)	124, 571, 727
SsiI	CCGC	7 cut(s)	86, 89, 185, 231, 664, 1450, 2824
SspI	AATATT	2 cut(s)	291, 1228
SspMI	CTAG	9 cut(s)	554, 824, 1215, 1596, 1847, 2175, 2460, 2748, 2760
SstI	GAGCTC	4 cut(s)	631, 690, 2209, 2371
StuI	AGGCCT	3 cut(s)	124, 571, 727
StyD4I	CCNGG	5 cut(s)	110, 243, 267, 1949, 2805
StyI	CCWWGG	2 cut(s)	297, 1419
TaaI	ACNGT	4 cut(s)	968, 2467, 2495, 2866
TaiI	ACGT	6 cut(s)	278, 594, 1876, 2108, 2503, 2691
TaqI	TCGA	6 cut(s)	130, 510, 699, 903, 1726, 2574
TatI	WGTACW	1 cut(s)	2753
TauI	GCSGC	4 cut(s)	88, 91, 667, 2826
TfiI	GAWTC	7 cut(s)	47, 932, 1090, 1382, 1616, 2346, 2426
TscAI	CASTG	5 cut(s)	876, 999, 1035, 1738, 2500
TseFI	GTSAC	5 cut(s)	106, 397, 406, 1253, 2717
Tsp45I	GTSAC	5 cut(s)	106, 397, 406, 1253, 2717
TspGWI	ACGGA	5 cut(s)	51, 214, 410, 1535, 2339
TspRI	CASTG	5 cut(s)	876, 999, 1035, 1738, 2500
Vha464I	CTTAAG	1 cut(s)	2728
VpaK11BI	GGWCC	4 cut(s)	1064, 1289, 1340, 1953
XagI	CCTNNNNNAGG	1 cut(s)	576
XapI	RAATTY	4 cut(s)	705, 1115, 1919, 2379
XbaI	TCTAGA	2 cut(s)	553, 1846
XceI	RCATGY	5 cut(s)	13, 322, 1664, 2513, 2777
XcmI	CCANNNNNNNNNTGG	1 cut(s)	483
XmiI	GTMKAC	2 cut(s)	138, 2305
XspI	CTAG	9 cut(s)	554, 824, 1215, 1596, 1847, 2175, 2460, 2748, 2760
ZrmI	AGTACT	1 cut(s)	2755
Zsp2I	ATGCAT	1 cut(s)	1141

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.