Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0007375

Physical Location & Seq

Genomic Coordinates Reverse (-)

6020073 .. 6027253

Gene Structure 7181 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG069300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2730 bp

ATGTTCATTTATAATCAGATGGGTGGTATAGATGAAGCTTCACTTGATCGTCTTGCTCTGGTCACCCAAGTGACCAAACATATCCGTGTTAAAGCCTCTGGAGGAAGAACCACAGCCTCTGAAATCGGGCAGTTCTCCCCAGTTTTTGTCTGGCTTCTAAGGGACTTCTATCTCGAATTAGTAGATAATGGTAGAAAGATAACACCCCGTGAGTATCTAGAGACTGCTTTGAGGCCTTTTGAAGGAAAACAAGATGTAGCTGCCAAAAATGAGATACGGGACTCCATTAGAGCTCTATTTCCAGATAGAGAATGCTTCACCCTTTTGCGACCTGTGGACAAGGAAGAGGAGCTCCGGAGACTCGATCAAATTCAGTTGAACAAGTTAAGGCCTGAATTTAAGTCAGGGCTGGATGCTTTGACAAGGTTTGTTTTTGAACGAACGAGGCCCAAGCAAGTTGGGGCCACTATGATGACAGGGCCAGTTCTAGTTGGTATCCTTCAGTCCTATCTTGATGCTCTTAATAATGGTGCAGTGCCTACCATCTCATCTTCATGGCAGAGTGTCGAAGAAGCAGAGTGCCGAAGAGCATATGATTCTGCGGTTGATGCTTATAGGTCTGCTTTTGACCGTTCAAAGTTGCCTGAAGAAGCTGCACTGAGGGAAGCTCATGAAGAGGCAGTCCAAAAATCACTGGCTGCTTTTAACGATTGTGCTGTAGGGGGTGGTCCAACAAGGAAAAAATATGAAGGGAATCTTCACAAACAGCTCAAAAAGGAATTTCAGGATTATAAGAAGAATGCATATATGGAGGCAGAGTTGCAATGTATAAATGCTATACAAAGGATGGAAGGGAAGCTGAGGAGCGCTTGCCATGCTAGTGATGCTAATATTGATAATGTTCTGAAGATTCTTGGTGACCTTATGTCTGAGTATGAAAAGGCAAGTCGGGGTCCACTAAAATGGCAGCAACTGGCTTTCTTTTTAAGAAAGAGTTTGGAAGGTCCAATTCTTGATCTCATCATGAGGCAAATCAACAAAGTTGAATCAGAGAATGGCACCTTGAGGTTGAGATCCCGTGCCATGCAAGATGAGTTGGGTTTACTGAAAAAGCGGGTAGAGGAAAGCAAGCAGTCTAAGATGGAGTACGTCAAACGCTACGAGGATGCTCTCAATGACAAGAACAAGCTAAATGATGAATACAATATCCGTTTTAACAACTTGCAAGGAAATAATACTTCTCTGCGAGATCAGTGTGCTAGCTTGCGTAAGAGTTTGGATTCTGTAAAAGCAGAAGCAGGAGAATGGCAAAGAAAATATGAGCAGGTTCTCTCCAAGCAGAAGGCTGAGGAAGATCAAGCTGGTTCTGAAATTGCGGTTCTCAAGTCTCGATGCAGTGCTGGTGAAGCAAGGCTGGCTGCTGCAAAGGAACAAGCTCAGTCTGCTCAAGAGGAGGCAGAGGACTGGAAACGCAAATATGACATTGCTTTTAGAGAGGCAAAAGCTGCTCTAGAGAAGGCAGCAATTGTCCAAGAGCGTTCCAGTAAGGAAACACAAAGGAGAGAAGATGCTTTGAGGGAAGAATTCACAAGTAGTTTGGCTGCAAAGGAGGAAGACATAAAGGAAAAGACAGCAAAGATTGAATATGCTGAGCAGTGCTTAACGACTCTAAAAATGGAACTAAAGGCTGCCAGGTCCAAAATTGATAGTTACGATGCTGAAATATCATCAGGAAAGCTTGAAATCAAGGAGCTGAGTAGAAAATTAGAAGCTGCAAATGACAAGGCCAATTCATTTGAAAGAGAAAAGAAGATACTGGAGCAGGAGAAGATCCATCTAAAGCAGACATATGAAACTGAGATCAAAAGATTGGATGAAGTCCAAGAAAGGTGTAAAATTGCTGAAAAAGAAGCCACTAGAGCAACTGATATTGCTGATAAAGCACGAGCTCAAGCAGACATAGCTCAAAAGGAAAAGGGGGAGATGCAGAGGCTGGCAATAGAAAGGTTGGCTCAAATTGAGAGGGCTGAGAGGCATATTGAAAGTCTACAGAGGGAAAAAAGAGACTTGGAAGTTGAGTTGGACGGAATCCGTGCATCAGAAAGGGGAGCTCATCACAAAATTTCTCTACTGGAGGCAAGAGTTGAAGAAAGGGAGAAAGAAATAGAATCATTATTGGAGTCGAACAATGAGCAGAGAACTAGTACAGTTCAAGTCCTTCAAGGTCTTCTGGACAGTGAACGTGCAGCACATGCAGACGCAAACAATAGGGCAGAAGCCCTCTCTCATCAGCTACAATCTGCACAAGCAAAACTCGATAAACTCCAGCAAGAATTGACTTCTGTTCGCCTGAATGAAACTGCATTGGATAGTAAGCTTAGGACTGCTTCACATGGGAAGCGTTCAAGGGTAGATGACTATGACATGGACGTGGACTCTGTTCAAGACGGGGAATTGAGTGACCGGATCTTAAGAGTAAATAAGAAGTCTAGGAGTACTACTAGCCCTCTGAAGCATGCGCAAACAGAAGATGGTGGTTCAGTATTCCGGGGAGATGAGGATAGCCGCAGTCAGCAAACCAATCCAGAAGATTACACAAAGTTCACAGTCCAGAAACTGAAGCAAGAGCTAACAAAGCATAATTTTGGTGCTGAGCTGCTGCAGTTAAGGAACCCCAACAAAAGGGAGATCCTCACGCTATATGAGAAGTGCGTTCTTCAAAAGTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0008104 GO:0008150 GO:0009987 GO:0010033 GO:0012505 GO:0015629 GO:0017076 GO:0019001 GO:0019221 GO:0023052 GO:0031974 GO:0031981 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0033036 GO:0033365 GO:0034097 GO:0034340 GO:0034341 GO:0034504 GO:0034612 GO:0034613 GO:0035639 GO:0036094 GO:0042221 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046983 GO:0048471 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051641 GO:0051716 GO:0060333 GO:0060337 GO:0065007 GO:0070013 GO:0070555 GO:0070727 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071347 GO:0071356 GO:0071357 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04621 map04621

Pfam Domains

Protein Families

GBP GBP_C

Protein Analysis

909

Amino Acids

103.78

Weight (kDa)

6.61

Isoelectric Point (pI)

54.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GBP	PF02263	2 - 148	8.9e-29	Guanylate-binding protein, N-terminal domain
GBP_C	PF02841	154 - 487	1.7e-30	Guanylate-binding protein, C-terminal domain
Tropomyosin	PF00261	422 - 630	9.5e-07	Tropomyosin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46070
fragaria_vesca	FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471
malus_domestica	MD05G1309600.v1.1 MD10G1288200.v1.1
prunus_persica	Prupe.4G053400_v2.0.a1
pyrus_communis	pycom05g28600 pycom10g24130 pycom10g24140
rosa_chinensis	RchiOBHm_Chr5g0009341
rosa_laevigata	RLG00000031653
rosa_multiflora	Rmu_sc0002711.1_g000023 Rmu_sc0009480.1_g000002
rosa_roxburghii	Rroxscaffold_1G00066750 Rroxscaffold_1G00067450
rosa_rugosa	Rorug04G0441800
rosa_samantha	Rh5AG072700 Rh5BG069000 Rh5CG081600 Rh5DG069300
rosa_wichuraiana	Rw5G006790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	12, 792
Acc16I	TGCGCA	1 cut(s)	2520
Acc36I	ACCTGC	1 cut(s)	1315
AccB1I	GGYRCC	1 cut(s)	1058
AccI	GTMKAC	1 cut(s)	2047
AccIII	TCCGGA	1 cut(s)	354
AciI	CCGC	4 cut(s)	602, 1114, 1376, 2566
AclWI	GGATC	4 cut(s)	1068, 1825, 2474, 2683
AcsI	RAATTY	5 cut(s)	369, 395, 779, 1583, 2121
AcuI	CTGAAG	5 cut(s)	485, 666, 926, 2531, 2639
AdeI	CACNNNGTG	1 cut(s)	209
AfaI	GTAC	3 cut(s)	1148, 2206, 2497
AfeI	AGCGCT	1 cut(s)	868
AfiI	CCNNNNNNNGG	2 cut(s)	242, 2682
AflII	CTTAAG	1 cut(s)	2470
AhdI	GACNNNNNGTC	1 cut(s)	925
AhlI	ACTAGT	1 cut(s)	2201
AjiI	CACGTC	1 cut(s)	2431
AjnI	CCWGG	1 cut(s)	1691
AleI	CACNNNNGTG	1 cut(s)	68
Alw21I	GWGCWC	4 cut(s)	295, 354, 1951, 2113
Alw26I	GTCTC	4 cut(s)	215, 352, 1392, 2058
AlwI	GGATC	4 cut(s)	1068, 1825, 2474, 2683
AlwNI	CAGNNNCTG	4 cut(s)	119, 973, 1993, 2617
Aor13HI	TCCGGA	1 cut(s)	354
Aor51HI	AGCGCT	1 cut(s)	868
AoxI	GGCC	6 cut(s)	233, 389, 446, 462, 479, 1785
ApoI	RAATTY	5 cut(s)	369, 395, 779, 1583, 2121
AspLEI	GCGC	2 cut(s)	869, 2521
AspS9I	GGNCC	7 cut(s)	447, 462, 479, 728, 953, 1004, 1695
AsuC2I	CCSGG	1 cut(s)	2549
AsuHPI	GGTGA	4 cut(s)	55, 310, 929, 1415
AsuNHI	GCTAGC	1 cut(s)	1259
AvaII	GGWCC	4 cut(s)	728, 953, 1004, 1695
BanI	GGYRCC	1 cut(s)	1058
BanII	GRGCYC	4 cut(s)	295, 354, 1951, 2113
BauI	CACGAG	1 cut(s)	1944
BbsI	GAAGAC	2 cut(s)	1620, 2219
Bbv12I	GWGCWC	4 cut(s)	295, 354, 1951, 2113
BbvCI	CCTCAGC	2 cut(s)	860, 1347
BccI	CCATC	6 cut(s)	13, 551, 841, 1135, 1842, 2525
BciT130I	CCWGG	1 cut(s)	1693
BciVI	GTATCC	1 cut(s)	506
BcnI	CCSGG	1 cut(s)	2549
BcoDI	GTCTC	4 cut(s)	215, 352, 1392, 2058
BcuI	ACTAGT	1 cut(s)	2201
BfaI	CTAG	9 cut(s)	218, 488, 879, 1260, 1511, 1917, 2202, 2490, 2502
BfmI	CTRYAG	3 cut(s)	717, 2048, 2660
BfoI	RGCGCY	1 cut(s)	870
BfrI	CTTAAG	1 cut(s)	2470
BfuAI	ACCTGC	1 cut(s)	1315
BfuI	GTATCC	1 cut(s)	506
BlpI	GCTNAGC	2 cut(s)	1650, 2652
BmcAI	AGTACT	1 cut(s)	2497
Bme1390I	CCNGG	2 cut(s)	1693, 2549
Bme18I	GGWCC	4 cut(s)	728, 953, 1004, 1695
BmeRI	GACNNNNNGTC	1 cut(s)	925
BmgBI	CACGTC	1 cut(s)	2431
BmgT120I	GGNCC	7 cut(s)	447, 462, 479, 728, 953, 1004, 1695
BmiI	GGNNCC	4 cut(s)	463, 954, 1060, 2672
BmrFI	CCNGG	2 cut(s)	1693, 2549
BmrI	ACTGGG	1 cut(s)	134
BmtI	GCTAGC	1 cut(s)	1263
BmuI	ACTGGG	1 cut(s)	134
BpiI	GAAGAC	2 cut(s)	1620, 2219
BplI	GAGNNNNNCTC	2 cut(s)	652, 684
BpmI	CTGGAG	4 cut(s)	120, 1838, 2153, 2309
Bpu10I	CCTNAGC	3 cut(s)	860, 1347, 2378
Bpu1102I	GCTNAGC	2 cut(s)	1650, 2652
BpuEI	CTTGAG	4 cut(s)	1084, 1367, 1431, 1935
BpuMI	CCSGG	1 cut(s)	2549
BsaJI	CCNNGG	1 cut(s)	2548
BsaWI	WCCGGW	2 cut(s)	354, 2463
BsaXI	ACNNNNNCTCC	2 cut(s)	803, 833
Bsc4I	CCNNNNNNNGG	2 cut(s)	242, 2682
Bse1I	ACTGG	8 cut(s)	140, 482, 699, 978, 1469, 1542, 1821, 2136
Bse3DI	GCAATG	2 cut(s)	830, 1482
BseAI	TCCGGA	1 cut(s)	354
BseBI	CCWGG	1 cut(s)	1693
BseDI	CCNNGG	1 cut(s)	2548
BseGI	GGATG	4 cut(s)	418, 852, 1171, 1879
BseLI	CCNNNNNNNGG	2 cut(s)	242, 2682
BseMI	GCAATG	2 cut(s)	830, 1482
BseNI	ACTGG	8 cut(s)	140, 482, 699, 978, 1469, 1542, 1821, 2136
BseRI	GAGGAG	3 cut(s)	362, 877, 1466
BsgI	GTGCAG	4 cut(s)	552, 639, 2265, 2286
BshFI	GGCC	6 cut(s)	235, 391, 448, 464, 481, 1787
BshNI	GGYRCC	1 cut(s)	1058
BsiHKAI	GWGCWC	4 cut(s)	295, 354, 1951, 2113
BsiSI	CCGG	3 cut(s)	355, 2464, 2548
BslFI	GGGAC	2 cut(s)	176, 293
BslI	CCNNNNNNNGG	2 cut(s)	242, 2682
BsmAI	GTCTC	4 cut(s)	215, 352, 1392, 2058
BsmFI	GGGAC	2 cut(s)	176, 293
BsmI	GAATGC	2 cut(s)	317, 805
BsnI	GGCC	6 cut(s)	235, 391, 448, 464, 481, 1787
Bsp1286I	GDGCHC	4 cut(s)	295, 354, 1951, 2113
Bsp13I	TCCGGA	1 cut(s)	354
Bsp1720I	GCTNAGC	2 cut(s)	1650, 2652
BspACI	CCGC	4 cut(s)	602, 1114, 1376, 2566
BspANI	GGCC	6 cut(s)	235, 391, 448, 464, 481, 1787
BspEI	TCCGGA	1 cut(s)	354
BspHI	TCATGA	2 cut(s)	670, 1023
BspLI	GGNNCC	4 cut(s)	463, 954, 1060, 2672
BspMAI	CTGCAG	1 cut(s)	2664
BspMI	ACCTGC	1 cut(s)	1315
BspOI	GCTAGC	1 cut(s)	1263
BspPI	GGATC	4 cut(s)	1068, 1825, 2474, 2683
BspQI	GCTCTTC	1 cut(s)	580
BspT107I	GGYRCC	1 cut(s)	1058
BspTI	CTTAAG	1 cut(s)	2470
BsrDI	GCAATG	2 cut(s)	830, 1482
BsrI	ACTGG	8 cut(s)	140, 482, 699, 978, 1469, 1542, 1821, 2136
BssECI	CCNNGG	1 cut(s)	2548
BssSI	CACGAG	1 cut(s)	1944
Bst2BI	CACGAG	1 cut(s)	1944
Bst2UI	CCWGG	1 cut(s)	1693
Bst4CI	ACNGT	4 cut(s)	632, 2209, 2237, 2608
Bst6I	CTCTTC	3 cut(s)	339, 580, 669
BstAFI	CTTAAG	1 cut(s)	2470
BstAPI	GCANNNNNTGC	2 cut(s)	1505, 2252
BstC8I	GCNNGC	7 cut(s)	871, 1130, 1261, 1265, 1416, 1995, 2517
BstEII	GGTNACC	2 cut(s)	61, 917
BstENI	CCTNNNNNAGG	1 cut(s)	240
BstF5I	GGATG	4 cut(s)	418, 852, 1171, 1879
BstH2I	RGCGCY	1 cut(s)	870
BstHHI	GCGC	2 cut(s)	869, 2521
BstMAI	GTCTC	4 cut(s)	215, 352, 1392, 2058
BstNI	CCWGG	1 cut(s)	1693
BstNSI	RCATGY	2 cut(s)	2255, 2519
BstPI	GGTNACC	2 cut(s)	61, 917
BstSCI	CCNGG	2 cut(s)	1691, 2547
BstSFI	CTRYAG	3 cut(s)	717, 2048, 2660
BstV2I	GAAGAC	2 cut(s)	1620, 2219
BstX2I	RGATCY	4 cut(s)	1073, 1830, 2466, 2688
BstXI	CCANNNNNNTGG	1 cut(s)	963
BstYI	RGATCY	4 cut(s)	1073, 1830, 2466, 2688
BsuI	GTATCC	1 cut(s)	506
BsuRI	GGCC	6 cut(s)	235, 391, 448, 464, 481, 1787
BtrI	CACGTC	1 cut(s)	2431
BtsCI	GGATG	4 cut(s)	418, 852, 1171, 1879
BtsI	GCAGTG	3 cut(s)	540, 1402, 1661
BtsIMutI	CAGTG	7 cut(s)	540, 656, 692, 1259, 1402, 1661, 2242
BveI	ACCTGC	1 cut(s)	1315
Cac8I	GCNNGC	7 cut(s)	871, 1130, 1261, 1265, 1416, 1995, 2517
CaiI	CAGNNNCTG	4 cut(s)	119, 973, 1993, 2617
CciI	TCATGA	2 cut(s)	670, 1023
CfoI	GCGC	2 cut(s)	869, 2521
Cfr13I	GGNCC	7 cut(s)	447, 462, 479, 728, 953, 1004, 1695
CseI	GACGC	1 cut(s)	2267
Csp6I	GTAC	3 cut(s)	1147, 2205, 2496
CviAII	CATG	9 cut(s)	555, 671, 875, 1024, 1084, 2252, 2393, 2425, 2516
CviQI	GTAC	3 cut(s)	1147, 2205, 2496
DraIII	CACNNNGTG	1 cut(s)	209
DriI	GACNNNNNGTC	1 cut(s)	925
Eam1104I	CTCTTC	3 cut(s)	339, 580, 669
Eam1105I	GACNNNNNGTC	1 cut(s)	925
EarI	CTCTTC	3 cut(s)	339, 580, 669
Ecl136II	GAGCTC	4 cut(s)	293, 352, 1949, 2111
Eco147I	AGGCCT	2 cut(s)	235, 391
Eco24I	GRGCYC	4 cut(s)	295, 354, 1951, 2113
Eco47I	GGWCC	4 cut(s)	728, 953, 1004, 1695
Eco47III	AGCGCT	1 cut(s)	868
Eco53kI	GAGCTC	4 cut(s)	293, 352, 1949, 2111
Eco57I	CTGAAG	5 cut(s)	485, 666, 926, 2531, 2639
Eco91I	GGTNACC	2 cut(s)	61, 917
EcoICRI	GAGCTC	4 cut(s)	293, 352, 1949, 2111
EcoNI	CCTNNNNNAGG	1 cut(s)	240
EcoO65I	GGTNACC	2 cut(s)	61, 917
EcoRI	GAATTC	1 cut(s)	1583
EcoRII	CCWGG	1 cut(s)	1691
EcoT22I	ATGCAT	1 cut(s)	805
EcoT38I	GRGCYC	4 cut(s)	295, 354, 1951, 2113
FaeI	CATG	9 cut(s)	558, 674, 878, 1027, 1087, 2255, 2396, 2428, 2519
FalI	AAGNNNNNCTT	4 cut(s)	27, 59, 741, 773
FaqI	GGGAC	2 cut(s)	176, 293
FatI	CATG	9 cut(s)	554, 670, 874, 1023, 1083, 2251, 2392, 2424, 2515
FauI	CCCGC	1 cut(s)	1107
FauNDI	CATATG	2 cut(s)	592, 1849
FblI	GTMKAC	1 cut(s)	2047
FokI	GGATG	4 cut(s)	425, 859, 1178, 1886
FriOI	GRGCYC	4 cut(s)	295, 354, 1951, 2113
FspBI	CTAG	9 cut(s)	218, 488, 879, 1260, 1511, 1917, 2202, 2490, 2502
FspI	TGCGCA	1 cut(s)	2520
GlaI	GCGC	2 cut(s)	868, 2520
GsuI	CTGGAG	4 cut(s)	120, 1838, 2153, 2309
HaeII	RGCGCY	1 cut(s)	870
HaeIII	GGCC	6 cut(s)	235, 391, 448, 464, 481, 1787
HapII	CCGG	3 cut(s)	355, 2464, 2548
HgaI	GACGC	1 cut(s)	2267
HhaI	GCGC	2 cut(s)	869, 2521
Hin1II	CATG	9 cut(s)	558, 674, 878, 1027, 1087, 2255, 2396, 2428, 2519
Hin6I	GCGC	2 cut(s)	867, 2519
HinP1I	GCGC	2 cut(s)	867, 2519
HindIII	AAGCTT	3 cut(s)	36, 1736, 2375
HpaII	CCGG	3 cut(s)	355, 2464, 2548
HphI	GGTGA	4 cut(s)	55, 310, 929, 1415
Hpy166II	GTNNAC	7 cut(s)	337, 956, 1103, 2048, 2240, 2434, 2604
Hpy188I	TCNGA	8 cut(s)	18, 121, 906, 931, 1051, 1369, 2101, 2511
Hpy8I	GTNNAC	7 cut(s)	337, 956, 1103, 2048, 2240, 2434, 2604
HpyAV	CCTTC	8 cut(s)	236, 509, 743, 845, 995, 1336, 1510, 2228
HpyCH4III	ACNGT	4 cut(s)	632, 2209, 2237, 2608
HpyCH4IV	ACGT	3 cut(s)	1149, 2242, 2430
HpySE526I	ACGT	3 cut(s)	1149, 2242, 2430
Hsp92II	CATG	9 cut(s)	558, 674, 878, 1027, 1087, 2255, 2396, 2428, 2519
HspAI	GCGC	2 cut(s)	867, 2519
Kpn2I	TCCGGA	1 cut(s)	354
LguI	GCTCTTC	1 cut(s)	580
LmnI	GCTCC	6 cut(s)	349, 357, 864, 1750, 1819, 2108
MaeI	CTAG	9 cut(s)	218, 488, 879, 1260, 1511, 1917, 2202, 2490, 2502
MaeII	ACGT	3 cut(s)	1149, 2242, 2430
MaeIII	GTNAC	5 cut(s)	61, 70, 917, 1709, 2459
MfeI	CAATTG	1 cut(s)	1524
MflI	RGATCY	4 cut(s)	1073, 1830, 2466, 2688
MhlI	GDGCHC	4 cut(s)	295, 354, 1951, 2113
MlyI	GAGTC	5 cut(s)	275, 354, 1660, 2189, 2429
MmeI	TCCRAC	2 cut(s)	755, 2061
Mph1103I	ATGCAT	1 cut(s)	805
MroI	TCCGGA	1 cut(s)	354
MslI	CAYNNNNRTG	7 cut(s)	68, 84, 470, 553, 879, 961, 2429
MspCI	CTTAAG	1 cut(s)	2470
MspI	CCGG	3 cut(s)	355, 2464, 2548
MspR9I	CCNGG	2 cut(s)	1693, 2549
MunI	CAATTG	1 cut(s)	1524
Mva1269I	GAATGC	2 cut(s)	317, 805
MvaI	CCWGG	1 cut(s)	1693
NciI	CCSGG	1 cut(s)	2549
NdeI	CATATG	2 cut(s)	592, 1849
NheI	GCTAGC	1 cut(s)	1259
NlaIII	CATG	9 cut(s)	558, 674, 878, 1027, 1087, 2255, 2396, 2428, 2519
NlaIV	GGNNCC	4 cut(s)	463, 954, 1060, 2672
NmuCI	GTSAC	4 cut(s)	61, 70, 917, 2459
NsbI	TGCGCA	1 cut(s)	2520
NsiI	ATGCAT	1 cut(s)	805
NspI	RCATGY	2 cut(s)	2255, 2519
OliI	CACNNNNGTG	1 cut(s)	68
PaeI	GCATGC	1 cut(s)	2519
PagI	TCATGA	2 cut(s)	670, 1023
PceI	AGGCCT	2 cut(s)	235, 391
PciSI	GCTCTTC	1 cut(s)	580
PctI	GAATGC	2 cut(s)	317, 805
PfeI	GAWTC	7 cut(s)	596, 754, 910, 1046, 1280, 2088, 2168
PleI	GAGTC	5 cut(s)	275, 354, 1660, 2188, 2429
PpsI	GAGTC	5 cut(s)	275, 354, 1660, 2188, 2429
PsiI	TTATAA	2 cut(s)	12, 792
Psp124BI	GAGCTC	4 cut(s)	295, 354, 1951, 2113
Psp6I	CCWGG	1 cut(s)	1691
PspEI	GGTNACC	2 cut(s)	61, 917
PspGI	CCWGG	1 cut(s)	1691
PspN4I	GGNNCC	4 cut(s)	463, 954, 1060, 2672
PspPI	GGNCC	7 cut(s)	447, 462, 479, 728, 953, 1004, 1695
PstI	CTGCAG	1 cut(s)	2664
PstNI	CAGNNNCTG	4 cut(s)	119, 973, 1993, 2617
PsuI	RGATCY	4 cut(s)	1073, 1830, 2466, 2688
RsaI	GTAC	3 cut(s)	1148, 2206, 2497
RsaNI	GTAC	3 cut(s)	1147, 2205, 2496
RseI	CAYNNNNRTG	7 cut(s)	68, 84, 470, 553, 879, 961, 2429
SacI	GAGCTC	4 cut(s)	295, 354, 1951, 2113
SapI	GCTCTTC	1 cut(s)	580
Sau96I	GGNCC	7 cut(s)	447, 462, 479, 728, 953, 1004, 1695
ScaI	AGTACT	1 cut(s)	2497
SchI	GAGTC	5 cut(s)	275, 354, 1660, 2189, 2429
ScrFI	CCNGG	2 cut(s)	1693, 2549
SduI	GDGCHC	4 cut(s)	295, 354, 1951, 2113
SfcI	CTRYAG	3 cut(s)	717, 2048, 2660
SinI	GGWCC	4 cut(s)	728, 953, 1004, 1695
SmiMI	CAYNNNNRTG	7 cut(s)	68, 84, 470, 553, 879, 961, 2429
SmlI	CTYRAG	5 cut(s)	1063, 1382, 1446, 1950, 2470
SmoI	CTYRAG	5 cut(s)	1063, 1382, 1446, 1950, 2470
SpeI	ACTAGT	1 cut(s)	2201
SphI	GCATGC	1 cut(s)	2519
SseBI	AGGCCT	2 cut(s)	235, 391
SsiI	CCGC	4 cut(s)	602, 1114, 1376, 2566
SspI	AATATT	1 cut(s)	892
SspMI	CTAG	9 cut(s)	218, 488, 879, 1260, 1511, 1917, 2202, 2490, 2502
SstI	GAGCTC	4 cut(s)	295, 354, 1951, 2113
StuI	AGGCCT	2 cut(s)	235, 391
StyD4I	CCNGG	2 cut(s)	1691, 2547
TaaI	ACNGT	4 cut(s)	632, 2209, 2237, 2608
TaiI	ACGT	3 cut(s)	1152, 2245, 2433
TaqI	TCGA	6 cut(s)	174, 363, 567, 1390, 2183, 2316
TatI	WGTACW	2 cut(s)	2204, 2495
TauI	GCSGC	1 cut(s)	2568
TfiI	GAWTC	7 cut(s)	596, 754, 910, 1046, 1280, 2088, 2168
TscAI	CASTG	7 cut(s)	540, 663, 699, 1259, 1402, 1661, 2242
TseFI	GTSAC	4 cut(s)	61, 70, 917, 2459
Tsp45I	GTSAC	4 cut(s)	61, 70, 917, 2459
TspGWI	ACGGA	4 cut(s)	74, 1199, 2081, 2100
TspRI	CASTG	7 cut(s)	540, 663, 699, 1259, 1402, 1661, 2242
Vha464I	CTTAAG	1 cut(s)	2470
VpaK11BI	GGWCC	4 cut(s)	728, 953, 1004, 1695
XagI	CCTNNNNNAGG	1 cut(s)	240
XapI	RAATTY	5 cut(s)	369, 395, 779, 1583, 2121
XbaI	TCTAGA	2 cut(s)	217, 1510
XceI	RCATGY	2 cut(s)	2255, 2519
XcmI	CCANNNNNNNNNTGG	1 cut(s)	147
XmiI	GTMKAC	1 cut(s)	2047
XspI	CTAG	9 cut(s)	218, 488, 879, 1260, 1511, 1917, 2202, 2490, 2502
ZrmI	AGTACT	1 cut(s)	2497
Zsp2I	ATGCAT	1 cut(s)	805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.