Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0007375

Physical Location & Seq

Genomic Coordinates Forward (+)

88752997 .. 88759967

Gene Structure 6971 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00067450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2991 bp

ATGTTTCGCATGTTCAGAGGAGGCAACGCCTCCGATGCCTCGCCGGAATCTTCCTCCTCTCCGTCGTCGTCGTCCTCGCCTTCGCCGCCGCAGCCGTCTCGGCCGGTGACCGGGCCGGCGAGGCCTATTCGACTCGTCTACGCCGACGAGAAAGGGAGGTTCCGGATGGATCCGGAAGCCGTCGCGGTGTTGCAGCTCGTTAAGGAGCCGATTGGTGTCGTCTCCGTCTGCGGTCGCGCTCGCCAGGGCAAGAGCTATATTCTCAACCAGGGAACGTACAGCACACAAATATTTTCCTTGGCTGTTCTCTTATCCAGCATGTTCATTTATAATCAGGTTAGGAGACTTCTGCTGATGGGTGGTATAGATGAAGCTTCACTTGATCGTCTTGCTCTTGTCACCCAAGTGACCAAACATATCCGTGTTAAAGCCTCTGGAGGAAGAACCACAGCCTCTGAAATCGGGCAGTTCTCCCCAGTTTTTGTCTGGCTTCTAAGGGACTTCTATCTCGAATTAGTAGATAATGGTAGAAAGATAACACCCCGTGAGTATCTAGAGACTGCTTTGAGGCCTTTTGAAGGAAAACAAGACGTAGCTGCCAAAAATGAGATACGGGACTCCATTAGAGCTCTATTTCCAGATAGAGAATGCTTCACCCTTTTGCGGCCTGTGGACAAGGAAGAGGAGCTCCGGAGACTCGATCAAATTCAGTTGAACAAGTTAAGGCCTGAGTTTAGGTCAGGGCTGGATGCTTTGACAAGTTTTGTTTTTAAACGAACGAGGCCCAAGCAAGTTGGGGCCACTATGATGACAGGGCCAGTTCTAGTTGGTATCCTTCAGTCCTATCTTGATGCTCTTAATAATGGTGCAGTGCCTACCATCTCATCTTCATGGCAGAGTGTCGAAGAAGCAGAGTGCCGAAGAGCATATGATTCTGCTGTTGATGCTTATAGGTCTGCTTTTGACCGTTCAAAGTTGCCTGAAGAAGTAAGCTGCACTGAGGAGGCTCATGAAGAGGCAGTCCAAAAATCACTGGCTGCTTTTAATGATTGTGCTGTAGGGGGTGGTCCAACAAGGAAAAAATATGAAGGGAATCTTCACAAACAGCTCAAAAAGGAATTTGAAGATTATAAGAAGAATGCATATATGGAGGCAGAGTTGCAATGTGTAAATGCTATACAAAGGATGGAAGGGAAGCTGAGGAGCGCTTGCCATGCTAGTGATGCTAATATTGATAATGTTCTGAAGGTTCTTGGTGACCTTATGTCCGAGTATGAAAAGGCAAGTAGGGGTCCACTAAAATGGCAGCAACTGGCTTTCTTTTTAAGAAAGAGTTTGGAAGGTCCAATTCTTGATCTCATCATGAGGCAAATCAACAAAGTTGAATCACAGAATGGCACCTTGAGGTTGAGATCCCGTGCCATGGAAGATGAGTTGGGTTTACTAAAAAAGCGGGTAGAGGAAAGCAAGCAGTCTAAGATGGAGTATGTCAAACGCTACGAGGACGCTCTCAATGACAATAACAAGCTAAAGGATGAATACAATGTCCGTATTAACAACTTGCAAGGAAATAATACTTCTCTGCGAGATAAGTGTGCTAGCTTGCGTAAGAGTTTGGATTCTGTAAAAGCAGAAGCAGGAGAATGGCAAAGAAAATATGAGCATGTTCTCTCCAAGCAGAAGGCTGAGGAAGATCAAGCTGGTTCCGAAATCGCTGTTCTCAAGTCTCGATGCAGTGCTGGCGAAGCAAGGCTGGCTGCTGCAAAGGAACAAGCTCAGTCTGCCCAAGAGGAGGCAGAGGACTGGAAACGCAAATATGACATTGCTTTTAGAGAGGCAAAAGCTGCTCTAGAGAAGGCAGCAATTGTCCAAGAACGTTCCAGTAAGGAAACACAAAGGAGAGAAGATGCTTTGAGGGAAGAATTCACAAGTAGTTTGGCTGCGAAGGCTGCCAGGTCCAAAATTGATAGTTATGATGCTGAAATATCATCAGGAAAGCTTGAAATCAAGGAGCTGAGTAGAAAATTAGAAGCAGCAAATGACAAGGCCAATTCATTTGAAAGAGAAAAGAAGATACTGGAGCAGGAGAAGATCCATCTAAAGCAGACGTATGAAACTGAGATCAAAAGATTGGATGAAGTCCAAGAAAGGTGTAAAATTGCTGAAAAAGAAGCCACTAGAGCAACTGATATTGCTGATAAAGCACGAGCTCAAGCAGACATAGCTCAAAAGGAAAAGGGGGAGATGCAGAGGCTGGCAATAGAAAGGTTGGCTCAAATTGAGAGGGCTGAGAGGCATATTGAAAGTCTACAGAGGGAGAAAAGAGACTTGGAAGTTGAGTTGGAAGGAATCCGTGCATCAGAAAGGGGAGCTCATCACAAAATTTCTCTACTGGAGGCAAGAGTTGAGGAAAGGGAGAAAGAAATAGAATCATTATTGGAGTCAAACAATGAGCAGAGAACTAGCACAGTTCAAGTCCTTCAAGGTCTTCTGGACAGTGAACGTGCAGCACATGCAGACGCAAACAATAGGGCAGAAGCCCTCTCTCATCAGCTACAATCTGCACAAGCAAAACTCGATAAACTCCAGCAAGAATTGACTTCTGTTCGCCTGAATGAAACTGCATTGGATAGTAAGCTTAGGACTGCTTCACATGGGAAGCGTTCAAGGGTAGATGACTATGACATGGACGTGGACTCTGTTCAAGATGGGGAATTGAGTGACCGGATCTTAAGAGTAAATAAGAAGTCTAGGAGTACTACTAGCCCTCTGAAGCATGCACAAACAGAAGATGGTGGTTCAGTATTCCGGGGAGATGAGGATAGCCGCAGTCAACAAACCAATCCAGAAGATTACACAAAGTTCACAGTCCAGAAACTGAAGCAAGAGCTAACAAAGCATAATTTCGGTGCTGAGCTGCTGCAGTTAAGGAACCCCAACAAAAGGGAGATCCTCACACTATATGAGAAGTGCATTCTTCAAAAGTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0008104 GO:0008150 GO:0009987 GO:0010033 GO:0012505 GO:0015629 GO:0017076 GO:0019001 GO:0019221 GO:0023052 GO:0031974 GO:0031981 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0033036 GO:0033365 GO:0034097 GO:0034340 GO:0034341 GO:0034504 GO:0034612 GO:0034613 GO:0035639 GO:0036094 GO:0042221 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046983 GO:0048471 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051641 GO:0051716 GO:0060333 GO:0060337 GO:0065007 GO:0070013 GO:0070555 GO:0070727 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071347 GO:0071356 GO:0071357 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04621 map04621

Pfam Domains

Protein Families

GBP GBP_C

Protein Analysis

996

Amino Acids

112.77

Weight (kDa)

8.33

Isoelectric Point (pI)

57.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GBP	PF02263	94 - 261	2.5e-33	Guanylate-binding protein, N-terminal domain
GBP_C	PF02841	266 - 570	1.4e-29	Guanylate-binding protein, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46070
fragaria_vesca	FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471 FvH4_3g08471
malus_domestica	MD05G1309600.v1.1 MD10G1288200.v1.1
prunus_persica	Prupe.4G053400_v2.0.a1
pyrus_communis	pycom05g28600 pycom10g24130 pycom10g24140
rosa_chinensis	RchiOBHm_Chr5g0009341
rosa_laevigata	RLG00000031653
rosa_multiflora	Rmu_sc0002711.1_g000023 Rmu_sc0009480.1_g000002
rosa_roxburghii	Rroxscaffold_1G00066750 Rroxscaffold_1G00067450
rosa_rugosa	Rorug04G0441800
rosa_samantha	Rh5AG072700 Rh5BG069000 Rh5CG081600 Rh5DG069300
rosa_wichuraiana	Rw5G006790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	330, 1131
AccB1I	GGYRCC	1 cut(s)	1397
AccI	GTMKAC	2 cut(s)	138, 2308
AccII	CGCG	2 cut(s)	185, 237
AccIII	TCCGGA	3 cut(s)	162, 172, 690
AciI	CCGC	7 cut(s)	86, 89, 185, 231, 664, 1453, 2827
AclI	AACGTT	1 cut(s)	1876
AclWI	GGATC	6 cut(s)	164, 177, 1407, 2086, 2735, 2944
AcoI	YGGCCR	1 cut(s)	101
AcsI	RAATTY	4 cut(s)	705, 1118, 1922, 2382
AcuI	CTGAAG	5 cut(s)	821, 1002, 1265, 2792, 2900
AdeI	CACNNNGTG	1 cut(s)	545
AfaI	GTAC	2 cut(s)	278, 2758
AfeI	AGCGCT	1 cut(s)	1207
AfiI	CCNNNNNNNGG	6 cut(s)	110, 578, 663, 1423, 1792, 2943
AflII	CTTAAG	1 cut(s)	2731
AhdI	GACNNNNNGTC	1 cut(s)	1264
AjiI	CACGTC	1 cut(s)	2692
AjnI	CCWGG	3 cut(s)	243, 267, 1952
AjuI	GAANNNNNNNTTGG	2 cut(s)	2312, 2344
AleI	CACNNNNGTG	1 cut(s)	404
Alw21I	GWGCWC	4 cut(s)	631, 690, 2212, 2374
Alw26I	GTCTC	7 cut(s)	102, 226, 337, 551, 688, 1731, 2319
AlwI	GGATC	6 cut(s)	164, 177, 1407, 2086, 2735, 2944
AlwNI	CAGNNNCTG	4 cut(s)	455, 1312, 2254, 2878
Aor13HI	TCCGGA	3 cut(s)	162, 172, 690
Aor51HI	AGCGCT	1 cut(s)	1207
ApoI	RAATTY	4 cut(s)	705, 1118, 1922, 2382
AspLEI	GCGC	2 cut(s)	239, 1208
AspS9I	GGNCC	8 cut(s)	113, 783, 798, 815, 1067, 1292, 1343, 1956
AsuC2I	CCSGG	2 cut(s)	112, 2810
AsuHPI	GGTGA	4 cut(s)	118, 391, 646, 1268
AsuNHI	GCTAGC	1 cut(s)	1598
AvaII	GGWCC	4 cut(s)	1067, 1292, 1343, 1956
BamHI	GGATCC	1 cut(s)	169
BanI	GGYRCC	1 cut(s)	1397
BanII	GRGCYC	4 cut(s)	631, 690, 2212, 2374
BauI	CACGAG	1 cut(s)	2205
BbsI	GAAGAC	1 cut(s)	2480
Bbv12I	GWGCWC	4 cut(s)	631, 690, 2212, 2374
BbvCI	CCTCAGC	2 cut(s)	1199, 1686
BccI	CCATC	8 cut(s)	160, 349, 887, 1180, 1474, 2103, 2702, 2786
BceAI	ACGGC	2 cut(s)	79, 164
BcgI	CGANNNNNNTGC	2 cut(s)	80, 114
BciT130I	CCWGG	3 cut(s)	245, 269, 1954
BciVI	GTATCC	1 cut(s)	842
BcnI	CCSGG	2 cut(s)	112, 2810
BcoDI	GTCTC	7 cut(s)	102, 226, 337, 551, 688, 1731, 2319
BfaI	CTAG	9 cut(s)	554, 824, 1218, 1599, 1850, 2178, 2463, 2751, 2763
BfmI	CTRYAG	3 cut(s)	1056, 2309, 2921
BfoI	RGCGCY	1 cut(s)	1209
BfrI	CTTAAG	1 cut(s)	2731
BfuI	GTATCC	1 cut(s)	842
BglI	GCCNNNNNGGC	2 cut(s)	100, 121
BlpI	GCTNAGC	1 cut(s)	2913
BmcAI	AGTACT	1 cut(s)	2758
Bme1390I	CCNGG	5 cut(s)	112, 245, 269, 1954, 2810
Bme18I	GGWCC	4 cut(s)	1067, 1292, 1343, 1956
BmeRI	GACNNNNNGTC	1 cut(s)	1264
BmgBI	CACGTC	1 cut(s)	2692
BmgT120I	GGNCC	8 cut(s)	113, 783, 798, 815, 1067, 1292, 1343, 1956
BmiI	GGNNCC	8 cut(s)	161, 171, 207, 799, 1293, 1399, 1705, 2933
BmrFI	CCNGG	5 cut(s)	112, 245, 269, 1954, 2810
BmrI	ACTGGG	1 cut(s)	470
BmtI	GCTAGC	1 cut(s)	1602
BmuI	ACTGGG	1 cut(s)	470
BpiI	GAAGAC	1 cut(s)	2480
BpmI	CTGGAG	4 cut(s)	456, 2099, 2414, 2570
Bpu10I	CCTNAGC	3 cut(s)	1199, 1686, 2639
Bpu1102I	GCTNAGC	1 cut(s)	2913
BpuEI	CTTGAG	3 cut(s)	1423, 1706, 2196
BpuMI	CCSGG	2 cut(s)	112, 2810
BsaJI	CCNNGG	5 cut(s)	244, 268, 297, 1422, 2809
BsaWI	WCCGGW	4 cut(s)	162, 172, 690, 2724
BsaXI	ACNNNNNCTCC	2 cut(s)	1142, 1172
Bsc4I	CCNNNNNNNGG	6 cut(s)	110, 578, 663, 1423, 1792, 2943
Bse118I	RCCGGY	2 cut(s)	103, 115
Bse1I	ACTGG	8 cut(s)	476, 818, 1038, 1317, 1808, 1881, 2082, 2397
Bse3DI	GCAATG	2 cut(s)	1169, 1821
BseAI	TCCGGA	3 cut(s)	162, 172, 690
BseBI	CCWGG	3 cut(s)	245, 269, 1954
BseDI	CCNNGG	5 cut(s)	244, 268, 297, 1422, 2809
BseGI	GGATG	5 cut(s)	171, 754, 1191, 1540, 2140
BseLI	CCNNNNNNNGG	6 cut(s)	110, 578, 663, 1423, 1792, 2943
BseMI	GCAATG	2 cut(s)	1169, 1821
BseMII	CTCAG	9 cut(s)	720, 990, 1190, 1677, 1790, 2006, 2109, 2280, 2904
BseNI	ACTGG	8 cut(s)	476, 818, 1038, 1317, 1808, 1881, 2082, 2397
BseRI	GAGGAG	6 cut(s)	33, 46, 698, 1016, 1216, 1805
BseX3I	CGGCCG	1 cut(s)	101
BsgI	GTGCAG	4 cut(s)	888, 979, 2526, 2547
Bsh1236I	CGCG	2 cut(s)	185, 237
Bsh1285I	CGRYCG	2 cut(s)	104, 235
BshNI	GGYRCC	1 cut(s)	1397
BsiEI	CGRYCG	2 cut(s)	104, 235
BsiHKAI	GWGCWC	4 cut(s)	631, 690, 2212, 2374
BsiSI	CCGG	9 cut(s)	44, 104, 111, 116, 163, 173, 691, 2725, 2809
BslFI	GGGAC	2 cut(s)	512, 629
BslI	CCNNNNNNNGG	6 cut(s)	110, 578, 663, 1423, 1792, 2943
BsmAI	GTCTC	7 cut(s)	102, 226, 337, 551, 688, 1731, 2319
BsmBI	CGTCTC	2 cut(s)	102, 226
BsmFI	GGGAC	2 cut(s)	512, 629
BsmI	GAATGC	3 cut(s)	653, 1144, 2973
Bsp1286I	GDGCHC	4 cut(s)	631, 690, 2212, 2374
Bsp13I	TCCGGA	3 cut(s)	162, 172, 690
Bsp1720I	GCTNAGC	1 cut(s)	2913
Bsp19I	CCATGG	1 cut(s)	1422
BspACI	CCGC	7 cut(s)	86, 89, 185, 231, 664, 1453, 2827
BspCNI	CTCAG	9 cut(s)	721, 991, 1191, 1678, 1789, 2007, 2110, 2281, 2905
BspEI	TCCGGA	3 cut(s)	162, 172, 690
BspFNI	CGCG	2 cut(s)	185, 237
BspHI	TCATGA	2 cut(s)	1009, 1362
BspLI	GGNNCC	8 cut(s)	161, 171, 207, 799, 1293, 1399, 1705, 2933
BspMAI	CTGCAG	1 cut(s)	2925
BspOI	GCTAGC	1 cut(s)	1602
BspPI	GGATC	6 cut(s)	164, 177, 1407, 2086, 2735, 2944
BspQI	GCTCTTC	1 cut(s)	916
BspT107I	GGYRCC	1 cut(s)	1397
BspTI	CTTAAG	1 cut(s)	2731
BsrDI	GCAATG	2 cut(s)	1169, 1821
BsrFI	RCCGGY	2 cut(s)	103, 115
BsrI	ACTGG	8 cut(s)	476, 818, 1038, 1317, 1808, 1881, 2082, 2397
BssAI	RCCGGY	2 cut(s)	103, 115
BssECI	CCNNGG	5 cut(s)	244, 268, 297, 1422, 2809
BssSI	CACGAG	1 cut(s)	2205
BssT1I	CCWWGG	2 cut(s)	297, 1422
Bst2BI	CACGAG	1 cut(s)	2205
Bst2UI	CCWGG	3 cut(s)	245, 269, 1954
Bst4CI	ACNGT	4 cut(s)	968, 2470, 2498, 2869
Bst6I	CTCTTC	3 cut(s)	675, 916, 1008
BstAFI	CTTAAG	1 cut(s)	2731
BstAPI	GCANNNNNTGC	2 cut(s)	1844, 2513
BstDSI	CCRYGG	1 cut(s)	1422
BstEII	GGTNACC	2 cut(s)	106, 1256
BstENI	CCTNNNNNAGG	1 cut(s)	576
BstF5I	GGATG	5 cut(s)	171, 754, 1191, 1540, 2140
BstFNI	CGCG	2 cut(s)	185, 237
BstH2I	RGCGCY	1 cut(s)	1209
BstHHI	GCGC	2 cut(s)	239, 1208
BstMAI	GTCTC	7 cut(s)	102, 226, 337, 551, 688, 1731, 2319
BstMCI	CGRYCG	2 cut(s)	104, 235
BstNI	CCWGG	3 cut(s)	245, 269, 1954
BstNSI	RCATGY	5 cut(s)	13, 322, 1667, 2516, 2780
BstPI	GGTNACC	2 cut(s)	106, 1256
BstSCI	CCNGG	5 cut(s)	110, 243, 267, 1952, 2808
BstSFI	CTRYAG	3 cut(s)	1056, 2309, 2921
BstUI	CGCG	2 cut(s)	185, 237
BstV2I	GAAGAC	1 cut(s)	2480
BstX2I	RGATCY	5 cut(s)	169, 1412, 2091, 2727, 2949
BstXI	CCANNNNNNTGG	1 cut(s)	1302
BstYI	RGATCY	5 cut(s)	169, 1412, 2091, 2727, 2949
BstZI	CGGCCG	1 cut(s)	101
BsuI	GTATCC	1 cut(s)	842
BtgI	CCRYGG	1 cut(s)	1422
BtrI	CACGTC	1 cut(s)	2692
BtsCI	GGATG	5 cut(s)	171, 754, 1191, 1540, 2140
BtsI	GCAGTG	2 cut(s)	876, 1741
BtsIMutI	CAGTG	5 cut(s)	876, 996, 1031, 1741, 2503
CaiI	CAGNNNCTG	4 cut(s)	455, 1312, 2254, 2878
CciI	TCATGA	2 cut(s)	1009, 1362
CfoI	GCGC	2 cut(s)	239, 1208
Cfr10I	RCCGGY	2 cut(s)	103, 115
Cfr13I	GGNCC	8 cut(s)	113, 783, 798, 815, 1067, 1292, 1343, 1956
CseI	GACGC	2 cut(s)	1514, 2528
Csp6I	GTAC	2 cut(s)	277, 2757
CviQI	GTAC	2 cut(s)	277, 2757
DraI	TTTAAA	1 cut(s)	772
DraIII	CACNNNGTG	1 cut(s)	545
DriI	GACNNNNNGTC	1 cut(s)	1264
EaeI	YGGCCR	1 cut(s)	101
EagI	CGGCCG	1 cut(s)	101
Eam1104I	CTCTTC	3 cut(s)	675, 916, 1008
Eam1105I	GACNNNNNGTC	1 cut(s)	1264
EarI	CTCTTC	3 cut(s)	675, 916, 1008
Ecl136II	GAGCTC	4 cut(s)	629, 688, 2210, 2372
EclXI	CGGCCG	1 cut(s)	101
Eco130I	CCWWGG	2 cut(s)	297, 1422
Eco147I	AGGCCT	3 cut(s)	124, 571, 727
Eco24I	GRGCYC	4 cut(s)	631, 690, 2212, 2374
Eco47I	GGWCC	4 cut(s)	1067, 1292, 1343, 1956
Eco47III	AGCGCT	1 cut(s)	1207
Eco52I	CGGCCG	1 cut(s)	101
Eco53kI	GAGCTC	4 cut(s)	629, 688, 2210, 2372
Eco57I	CTGAAG	5 cut(s)	821, 1002, 1265, 2792, 2900
Eco91I	GGTNACC	2 cut(s)	106, 1256
EcoICRI	GAGCTC	4 cut(s)	629, 688, 2210, 2372
EcoNI	CCTNNNNNAGG	1 cut(s)	576
EcoO65I	GGTNACC	2 cut(s)	106, 1256
EcoRI	GAATTC	1 cut(s)	1922
EcoRII	CCWGG	3 cut(s)	243, 267, 1952
EcoT14I	CCWWGG	2 cut(s)	297, 1422
EcoT22I	ATGCAT	1 cut(s)	1144
EcoT38I	GRGCYC	4 cut(s)	631, 690, 2212, 2374
ErhI	CCWWGG	2 cut(s)	297, 1422
Esp3I	CGTCTC	2 cut(s)	102, 226
FalI	AAGNNNNNCTT	4 cut(s)	363, 395, 1080, 1112
FaqI	GGGAC	2 cut(s)	512, 629
FauI	CCCGC	1 cut(s)	1446
FauNDI	CATATG	1 cut(s)	928
FblI	GTMKAC	2 cut(s)	138, 2308
FokI	GGATG	5 cut(s)	178, 761, 1198, 1547, 2147
FriOI	GRGCYC	4 cut(s)	631, 690, 2212, 2374
FspBI	CTAG	9 cut(s)	554, 824, 1218, 1599, 1850, 2178, 2463, 2751, 2763
GlaI	GCGC	2 cut(s)	238, 1207
GsuI	CTGGAG	4 cut(s)	456, 2099, 2414, 2570
HaeII	RGCGCY	1 cut(s)	1209
HapII	CCGG	9 cut(s)	44, 104, 111, 116, 163, 173, 691, 2725, 2809
HgaI	GACGC	2 cut(s)	1514, 2528
HhaI	GCGC	2 cut(s)	239, 1208
Hin6I	GCGC	2 cut(s)	237, 1206
HinP1I	GCGC	2 cut(s)	237, 1206
HincII	GTYRAC	1 cut(s)	2834
HindII	GTYRAC	1 cut(s)	2834
HindIII	AAGCTT	3 cut(s)	372, 1997, 2636
HpaII	CCGG	9 cut(s)	44, 104, 111, 116, 163, 173, 691, 2725, 2809
HphI	GGTGA	4 cut(s)	118, 391, 646, 1268
Hpy166II	GTNNAC	9 cut(s)	139, 673, 1295, 1442, 2309, 2501, 2695, 2834, 2865
Hpy188I	TCNGA	8 cut(s)	17, 34, 457, 1245, 1270, 1708, 2362, 2772
Hpy8I	GTNNAC	9 cut(s)	139, 673, 1295, 1442, 2309, 2501, 2695, 2834, 2865
Hpy99I	CGWCG	5 cut(s)	67, 70, 73, 149, 185
HpyCH4III	ACNGT	4 cut(s)	968, 2470, 2498, 2869
HpyCH4IV	ACGT	6 cut(s)	275, 591, 1876, 2108, 2503, 2691
HpySE526I	ACGT	6 cut(s)	275, 591, 1876, 2108, 2503, 2691
HspAI	GCGC	2 cut(s)	237, 1206
Kpn2I	TCCGGA	3 cut(s)	162, 172, 690
KroI	GCCGGC	1 cut(s)	115
KroNI	GCCGGC	1 cut(s)	117
LguI	GCTCTTC	1 cut(s)	916
LmnI	GCTCC	7 cut(s)	205, 685, 693, 1203, 2011, 2080, 2369
MaeI	CTAG	9 cut(s)	554, 824, 1218, 1599, 1850, 2178, 2463, 2751, 2763
MaeII	ACGT	6 cut(s)	275, 591, 1876, 2108, 2503, 2691
MaeIII	GTNAC	5 cut(s)	106, 397, 406, 1256, 2720
MfeI	CAATTG	1 cut(s)	1863
MflI	RGATCY	5 cut(s)	169, 1412, 2091, 2727, 2949
MhlI	GDGCHC	4 cut(s)	631, 690, 2212, 2374
MlyI	GAGTC	5 cut(s)	126, 611, 690, 2450, 2690
MmeI	TCCRAC	2 cut(s)	1094, 2322
Mph1103I	ATGCAT	1 cut(s)	1144
MroI	TCCGGA	3 cut(s)	162, 172, 690
MroNI	GCCGGC	1 cut(s)	115
MslI	CAYNNNNRTG	7 cut(s)	404, 420, 806, 889, 1218, 1300, 2690
MspCI	CTTAAG	1 cut(s)	2731
MspI	CCGG	9 cut(s)	44, 104, 111, 116, 163, 173, 691, 2725, 2809
MspR9I	CCNGG	5 cut(s)	112, 245, 269, 1954, 2810
MunI	CAATTG	1 cut(s)	1863
Mva1269I	GAATGC	3 cut(s)	653, 1144, 2973
MvaI	CCWGG	3 cut(s)	245, 269, 1954
MvnI	CGCG	2 cut(s)	185, 237
NaeI	GCCGGC	1 cut(s)	117
NciI	CCSGG	2 cut(s)	112, 2810
NcoI	CCATGG	1 cut(s)	1422
NdeI	CATATG	1 cut(s)	928
NgoMIV	GCCGGC	1 cut(s)	115
NheI	GCTAGC	1 cut(s)	1598
NlaIV	GGNNCC	8 cut(s)	161, 171, 207, 799, 1293, 1399, 1705, 2933
NmeAIII	GCCGAG	1 cut(s)	79
NmuCI	GTSAC	5 cut(s)	106, 397, 406, 1256, 2720
NsiI	ATGCAT	1 cut(s)	1144
NspI	RCATGY	5 cut(s)	13, 322, 1667, 2516, 2780
OliI	CACNNNNGTG	1 cut(s)	404
PaeI	GCATGC	1 cut(s)	2780
PagI	TCATGA	2 cut(s)	1009, 1362
PceI	AGGCCT	3 cut(s)	124, 571, 727
PciSI	GCTCTTC	1 cut(s)	916
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PctI	GAATGC	3 cut(s)	653, 1144, 2973
PdiI	GCCGGC	1 cut(s)	117
PfeI	GAWTC	7 cut(s)	47, 932, 1093, 1385, 1619, 2349, 2429
PleI	GAGTC	5 cut(s)	126, 611, 690, 2449, 2690
PpsI	GAGTC	5 cut(s)	126, 611, 690, 2449, 2690
PsiI	TTATAA	2 cut(s)	330, 1131
Psp124BI	GAGCTC	4 cut(s)	631, 690, 2212, 2374
Psp1406I	AACGTT	1 cut(s)	1876
Psp6I	CCWGG	3 cut(s)	243, 267, 1952
PspEI	GGTNACC	2 cut(s)	106, 1256
PspGI	CCWGG	3 cut(s)	243, 267, 1952
PspN4I	GGNNCC	8 cut(s)	161, 171, 207, 799, 1293, 1399, 1705, 2933
PspPI	GGNCC	8 cut(s)	113, 783, 798, 815, 1067, 1292, 1343, 1956
PstI	CTGCAG	1 cut(s)	2925
PstNI	CAGNNNCTG	4 cut(s)	455, 1312, 2254, 2878
PsuI	RGATCY	5 cut(s)	169, 1412, 2091, 2727, 2949
RsaI	GTAC	2 cut(s)	278, 2758
RsaNI	GTAC	2 cut(s)	277, 2757
RseI	CAYNNNNRTG	7 cut(s)	404, 420, 806, 889, 1218, 1300, 2690
SacI	GAGCTC	4 cut(s)	631, 690, 2212, 2374
SapI	GCTCTTC	1 cut(s)	916
Sau96I	GGNCC	8 cut(s)	113, 783, 798, 815, 1067, 1292, 1343, 1956
ScaI	AGTACT	1 cut(s)	2758
SchI	GAGTC	5 cut(s)	126, 611, 690, 2450, 2690
ScrFI	CCNGG	5 cut(s)	112, 245, 269, 1954, 2810
SduI	GDGCHC	4 cut(s)	631, 690, 2212, 2374
SfcI	CTRYAG	3 cut(s)	1056, 2309, 2921
SfiI	GGCCNNNNNGGCC	1 cut(s)	121
SinI	GGWCC	4 cut(s)	1067, 1292, 1343, 1956
SmiMI	CAYNNNNRTG	7 cut(s)	404, 420, 806, 889, 1218, 1300, 2690
SmlI	CTYRAG	4 cut(s)	1402, 1721, 2211, 2731
SmoI	CTYRAG	4 cut(s)	1402, 1721, 2211, 2731
SphI	GCATGC	1 cut(s)	2780
SseBI	AGGCCT	3 cut(s)	124, 571, 727
SsiI	CCGC	7 cut(s)	86, 89, 185, 231, 664, 1453, 2827
SspI	AATATT	2 cut(s)	291, 1231
SspMI	CTAG	9 cut(s)	554, 824, 1218, 1599, 1850, 2178, 2463, 2751, 2763
SstI	GAGCTC	4 cut(s)	631, 690, 2212, 2374
StuI	AGGCCT	3 cut(s)	124, 571, 727
StyD4I	CCNGG	5 cut(s)	110, 243, 267, 1952, 2808
StyI	CCWWGG	2 cut(s)	297, 1422
TaaI	ACNGT	4 cut(s)	968, 2470, 2498, 2869
TaiI	ACGT	6 cut(s)	278, 594, 1879, 2111, 2506, 2694
TaqI	TCGA	6 cut(s)	130, 510, 699, 903, 1729, 2577
TatI	WGTACW	1 cut(s)	2756
TauI	GCSGC	4 cut(s)	88, 91, 667, 2829
TfiI	GAWTC	7 cut(s)	47, 932, 1093, 1385, 1619, 2349, 2429
TscAI	CASTG	5 cut(s)	876, 1003, 1038, 1741, 2503
TseFI	GTSAC	5 cut(s)	106, 397, 406, 1256, 2720
Tsp45I	GTSAC	5 cut(s)	106, 397, 406, 1256, 2720
TspGWI	ACGGA	5 cut(s)	51, 214, 410, 1538, 2342
TspRI	CASTG	5 cut(s)	876, 1003, 1038, 1741, 2503
Vha464I	CTTAAG	1 cut(s)	2731
VpaK11BI	GGWCC	4 cut(s)	1067, 1292, 1343, 1956
XagI	CCTNNNNNAGG	1 cut(s)	576
XapI	RAATTY	4 cut(s)	705, 1118, 1922, 2382
XbaI	TCTAGA	2 cut(s)	553, 1849
XceI	RCATGY	5 cut(s)	13, 322, 1667, 2516, 2780
XcmI	CCANNNNNNNNNTGG	1 cut(s)	483
XmiI	GTMKAC	2 cut(s)	138, 2308
XspI	CTAG	9 cut(s)	554, 824, 1218, 1599, 1850, 2178, 2463, 2751, 2763
ZrmI	AGTACT	1 cut(s)	2758
Zsp2I	ATGCAT	1 cut(s)	1144

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.