MD05G1313300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014040
Physical Location & Seq
Reverse (-)
44389432 .. 44391191
1760 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1313300.v1.1.491

Sequence Viewer

Length: 459 bp
ATGTCGACCGCCAAAGCGAGCAACAAGAAGCGCAGGACCGACCCGGAGATCGCCAAATCGGACCACCTCGATCTCGACCTCGACCTCTCCAATGATATCAAGGGAATCATGTCGGCGATTCATCAGATCCGAGAGAAGGCTCACAAGGACGGTCTGAAGAAGAACGAAGAGACGATTTCGAGTGTGGCAGCTGAGGTCAGGTCTGCCATTGATGAGCTCAAGTCCAAACTCGAAAAGGATAGGCAAAGTTTCGCTAAATCCCTCTCTAAGAGCTCAAAAGAGTGTGAAAATTGCTTGAAGACTGAAGCTGCAAAGTTCCAAGCACTTTATGAAAAGTTCTGCAAGGACCAAGCAAGCCATCTGCAGGCCTTGAAAGGAGTTATTTCGAACTATGAAGAAGAGAAGGAAAGGCTCTGCGCACGATACGAACAATTGACGAGAAAGGAGTATGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.37

Weight (kDa)

8.26

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33793 AT2G33793 AT2G33793
fragaria_vesca FvH4_3g09320
malus_domestica MD05G1313300.v1.1
prunus_persica Prupe.4G046300_v2.0.a1
pyrus_communis pycom05g29130
rosa_chinensis RchiOBHm_Chr5g0008281
rosa_laevigata RLG00000031558
rosa_roxburghii Rroxscaffold_1G00055470 Rroxscaffold_1G00068180
rosa_rugosa Rorug04G0434700
rosa_samantha Rh5AG063400 Rh5BG059900 Rh5CG070200 Rh5DG059400
rosa_wichuraiana Rw5G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 418
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 9
AclWI GGATC 1 cut(s) 121
AcuI CTGAAG 2 cut(s) 176, 324
AfiI CCNNNNNNNGG 2 cut(s) 136, 364
AgsI TTSAA 2 cut(s) 298, 373
AluBI AGCT 4 cut(s) 191, 217, 273, 308
AluI AGCT 4 cut(s) 191, 217, 273, 308
Alw21I GWGCWC 2 cut(s) 219, 275
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 121
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 2 cut(s) 188, 308
AspLEI GCGC 2 cut(s) 33, 419
AspS9I GGNCC 3 cut(s) 36, 61, 346
AsuC2I CCSGG 1 cut(s) 44
AsuII TTCGAA 1 cut(s) 386
AvaII GGWCC 3 cut(s) 36, 61, 346
BanII GRGCYC 2 cut(s) 219, 275
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 2 cut(s) 219, 275
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 200, 295
BccI CCATC 1 cut(s) 366
BcnI CCSGG 1 cut(s) 44
BcoDI GTCTC 1 cut(s) 164
BfmI CTRYAG 1 cut(s) 362
BisI GCNGC 2 cut(s) 189, 309
BlsI GCNGC 2 cut(s) 190, 310
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 3 cut(s) 36, 61, 346
BmgT120I GGNCC 3 cut(s) 36, 61, 346
BmrFI CCNGG 1 cut(s) 44
BpiI GAAGAC 1 cut(s) 305
Bpu10I CCTNAGC 1 cut(s) 192
Bpu14I TTCGAA 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 44
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 364
BseLI CCNNNNNNNGG 2 cut(s) 136, 364
BseMII CTCAG 1 cut(s) 183
BseXI GCAGC 2 cut(s) 200, 295
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 2 cut(s) 219, 275
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 2 cut(s) 136, 364
BsmAI GTCTC 1 cut(s) 164
BsmBI CGTCTC 1 cut(s) 164
BsnI GGCC 1 cut(s) 368
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 2 cut(s) 219, 275
Bsp143I GATC 3 cut(s) 48, 70, 126
BspACI CCGC 1 cut(s) 9
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 1 cut(s) 184
BspMAI CTGCAG 1 cut(s) 366
BspPI GGATC 1 cut(s) 121
BspT104I TTCGAA 1 cut(s) 386
BssMI GATC 3 cut(s) 48, 70, 126
Bst4CI ACNGT 1 cut(s) 152
Bst6I CTCTTC 2 cut(s) 162, 393
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 19, 355, 366
BstDEI CTNAG 2 cut(s) 192, 267
BstHHI GCGC 2 cut(s) 33, 419
BstKTI GATC 3 cut(s) 51, 73, 129
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 3 cut(s) 48, 70, 126
BstMCI CGRYCG 1 cut(s) 9
BstSCI CCNGG 1 cut(s) 42
BstSFI CTRYAG 1 cut(s) 362
BstV1I GCAGC 2 cut(s) 200, 295
BstV2I GAAGAC 1 cut(s) 305
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 1 cut(s) 368
Cac8I GCNNGC 3 cut(s) 19, 355, 366
CfoI GCGC 2 cut(s) 33, 419
Cfr13I GGNCC 3 cut(s) 36, 61, 346
CviAII CATG 1 cut(s) 109
CviJI RGCY 8 cut(s) 140, 191, 217, 273, 308, 357, 368, 412
CviKI_1 RGCY 8 cut(s) 140, 191, 217, 273, 308, 357, 368, 412
DdeI CTNAG 2 cut(s) 192, 267
DpnI GATC 3 cut(s) 50, 72, 128
DpnII GATC 3 cut(s) 48, 70, 126
Eam1104I CTCTTC 2 cut(s) 162, 393
EarI CTCTTC 2 cut(s) 162, 393
Ecl136II GAGCTC 2 cut(s) 217, 273
Eco147I AGGCCT 1 cut(s) 368
Eco24I GRGCYC 2 cut(s) 219, 275
Eco32I GATATC 1 cut(s) 97
Eco47I GGWCC 3 cut(s) 36, 61, 346
Eco53kI GAGCTC 2 cut(s) 217, 273
Eco57I CTGAAG 2 cut(s) 176, 324
EcoICRI GAGCTC 2 cut(s) 217, 273
EcoRV GATATC 1 cut(s) 97
EcoT38I GRGCYC 2 cut(s) 219, 275
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 1 cut(s) 112
FaiI YATR 4 cut(s) 110, 330, 393, 450
FatI CATG 1 cut(s) 108
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 189, 309
FriOI GRGCYC 2 cut(s) 219, 275
Fsp4HI GCNGC 2 cut(s) 189, 309
FspI TGCGCA 1 cut(s) 418
GlaI GCGC 2 cut(s) 32, 418
GluI GCNGC 2 cut(s) 189, 309
HaeIII GGCC 1 cut(s) 368
HapII CCGG 1 cut(s) 44
HhaI GCGC 2 cut(s) 33, 419
Hin1II CATG 1 cut(s) 112
Hin6I GCGC 2 cut(s) 31, 417
HinP1I GCGC 2 cut(s) 31, 417
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 2 cut(s) 105, 118
HpaII CCGG 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 5 cut(s) 61, 126, 131, 156, 458
Hpy188III TCNNGA 1 cut(s) 74
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 2 cut(s) 130, 397
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4V TGCA 3 cut(s) 311, 342, 364
HpyF3I CTNAG 2 cut(s) 192, 267
Hsp92II CATG 1 cut(s) 112
HspAI GCGC 2 cut(s) 31, 417
Kzo9I GATC 3 cut(s) 48, 70, 126
LpnPI CCDG 4 cut(s) 19, 57, 184, 350
Lsp1109I GCAGC 2 cut(s) 200, 295
MalI GATC 3 cut(s) 50, 72, 128
MboI GATC 3 cut(s) 48, 70, 126
MboII GAAGA 6 cut(s) 169, 172, 179, 310, 407, 410
MfeI CAATTG 1 cut(s) 431
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 219, 275
MluCI AATT 2 cut(s) 289, 431
MnlI CCTC 5 cut(s) 77, 89, 95, 187, 272
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 44
MunI CAATTG 1 cut(s) 431
NciI CCSGG 1 cut(s) 44
NdeII GATC 3 cut(s) 48, 70, 126
NlaIII CATG 1 cut(s) 112
NsbI TGCGCA 1 cut(s) 418
NspV TTCGAA 1 cut(s) 386
PceI AGGCCT 1 cut(s) 368
PfeI GAWTC 2 cut(s) 105, 118
PkrI GCNGC 2 cut(s) 190, 310
Psp124BI GAGCTC 2 cut(s) 219, 275
PspPI GGNCC 3 cut(s) 36, 61, 346
PstI CTGCAG 1 cut(s) 366
PsuI RGATCY 1 cut(s) 126
PvuII CAGCTG 1 cut(s) 191
SacI GAGCTC 2 cut(s) 219, 275
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 2 cut(s) 189, 309
Sau3AI GATC 3 cut(s) 48, 70, 126
Sau96I GGNCC 3 cut(s) 36, 61, 346
ScrFI CCNGG 1 cut(s) 44
SduI GDGCHC 2 cut(s) 219, 275
SetI ASST 9 cut(s) 69, 81, 87, 193, 198, 203, 219, 275, 310
SfcI CTRYAG 1 cut(s) 362
SfuI TTCGAA 1 cut(s) 386
SinI GGWCC 3 cut(s) 36, 61, 346
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 2 cut(s) 289, 431
SseBI AGGCCT 1 cut(s) 368
SsiI CCGC 1 cut(s) 9
SstI GAGCTC 2 cut(s) 219, 275
StuI AGGCCT 1 cut(s) 368
StyD4I CCNGG 1 cut(s) 42
TaaI ACNGT 1 cut(s) 152
TaqI TCGA 7 cut(s) 5, 69, 75, 81, 179, 231, 386
TaqII GACCGA 1 cut(s) 53
TasI AATT 2 cut(s) 289, 431
TfiI GAWTC 2 cut(s) 105, 118
TseI GCWGC 2 cut(s) 188, 308
TspDTI ATGAA 3 cut(s) 110, 345, 408
VpaK11BI GGWCC 3 cut(s) 36, 61, 346
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.