Rh5DG059400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014040
Physical Location & Seq
Forward (+)
5028498 .. 5030776
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG059400.1

Sequence Viewer

Length: 630 bp
ATGTCCACCGCCAAAGCCACCACCAGGAAGAGGCGCGCGGCCGAGCCCGAGCTCGCCAAATCGGCCAACCTCGATCTCGATCTCGATCTCGACCTCTCCAGTGATATCAAAGGAATCATGTCGGCTCTGCAACAGATCAGAGAGAAGGCGCACAAGGACGGACTCAAGAAGAACGAAGAGACCATTTCCAGTGTGGCTTCTGAAGTCAGGTTTGTGATTGATGAACTGAAGTCGAAAGTTGAGAAGGACAGGCAAAGTTTCGCGAAATCACTTTCAAAGAGCTCAAAAGAGTGTGAGAATTGCTTGAAGACTGAAACTACCAAGTTCCAAGCAGTTTACGAGAAATTCTGCAAGGATCAAGCGAACCATCTACAATCCCTGAAAGGTATCATTTCAAACTTTGAAGAAGAGAAGGAAAGACTATTCACTCGATATGAGCAACTCAGAAAGAGAGAGAGAACTATGATATCAGAGCATGAAAAAGCTTGCACCGATAAAATTTCCAAGTTAGAAGAGTCTCTGAAAAAGAAGAAGCAGGACAACAAAACTTTCAGCGTTTTGAGGAAAACTCTTGGTTCATTTCTGGACGATGATGCTTCAGATGAAGAAGGCGTCCCACTTGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

23.92

Weight (kDa)

7.61

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASY3-like PF20435 21 - 179 5.4e-07 Meiosis-specific protein ASY3-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33793 AT2G33793 AT2G33793
fragaria_vesca FvH4_3g09320
malus_domestica MD05G1313300.v1.1
prunus_persica Prupe.4G046300_v2.0.a1
pyrus_communis pycom05g29130
rosa_chinensis RchiOBHm_Chr5g0008281
rosa_laevigata RLG00000031558
rosa_roxburghii Rroxscaffold_1G00055470 Rroxscaffold_1G00068180
rosa_rugosa Rorug04G0434700
rosa_samantha Rh5AG063400 Rh5BG059900 Rh5CG070200 Rh5DG059400
rosa_wichuraiana Rw5G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 36, 38, 263
AciI CCGC 2 cut(s) 9, 38
AclWI GGATC 1 cut(s) 363
AcoI YGGCCR 2 cut(s) 39, 63
AcsI RAATTY 2 cut(s) 344, 498
AcuI CTGAAG 3 cut(s) 222, 248, 582
AcyI GRCGYC 1 cut(s) 612
AfiI CCNNNNNNNGG 2 cut(s) 24, 30
AgsI TTSAA 4 cut(s) 276, 307, 396, 404
AjnI CCWGG 1 cut(s) 23
AluBI AGCT 3 cut(s) 52, 282, 485
AluI AGCT 3 cut(s) 52, 282, 485
Alw21I GWGCWC 2 cut(s) 54, 284
Alw26I GTCTC 2 cut(s) 173, 522
AlwI GGATC 1 cut(s) 363
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 2 cut(s) 39, 63
ApoI RAATTY 2 cut(s) 344, 498
AspLEI GCGC 3 cut(s) 36, 38, 151
AvaI CYCGRG 1 cut(s) 47
BanII GRGCYC 3 cut(s) 48, 54, 284
BbsI GAAGAC 1 cut(s) 314
Bbv12I GWGCWC 2 cut(s) 54, 284
BccI CCATC 1 cut(s) 375
BciT130I CCWGG 1 cut(s) 25
BcoDI GTCTC 2 cut(s) 173, 522
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 47
BmrFI CCNGG 1 cut(s) 25
BmsI GCATC 1 cut(s) 583
BpiI GAAGAC 1 cut(s) 314
BplI GAGNNNNNCTC 2 cut(s) 553, 585
BpmI CTGGAG 1 cut(s) 82
BpuEI CTTGAG 1 cut(s) 149
BsaBI GATNNNNATC 2 cut(s) 78, 84
BsaHI GRCGYC 1 cut(s) 612
BsaI GGTCTC 1 cut(s) 173
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 30
Bse1I ACTGG 2 cut(s) 99, 189
Bse8I GATNNNNATC 2 cut(s) 78, 84
BseBI CCWGG 1 cut(s) 25
BseJI GATNNNNATC 2 cut(s) 78, 84
BseLI CCNNNNNNNGG 2 cut(s) 24, 30
BseMII CTCAG 1 cut(s) 457
BseNI ACTGG 2 cut(s) 99, 189
BsePI GCGCGC 1 cut(s) 34
BseX3I CGGCCG 1 cut(s) 39
Bsh1236I CGCG 3 cut(s) 36, 38, 263
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 2 cut(s) 41, 65
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 2 cut(s) 54, 284
BsiHKCI CYCGRG 1 cut(s) 47
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 2 cut(s) 24, 30
BsmAI GTCTC 2 cut(s) 173, 522
BsmFI GGGAC 1 cut(s) 599
BsnI GGCC 2 cut(s) 41, 65
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 3 cut(s) 48, 54, 284
Bsp143I GATC 5 cut(s) 73, 79, 85, 135, 355
Bsp68I TCGCGA 1 cut(s) 263
BspACI CCGC 2 cut(s) 9, 38
BspANI GGCC 2 cut(s) 41, 65
BspCNI CTCAG 1 cut(s) 456
BspFNI CGCG 3 cut(s) 36, 38, 263
BspPI GGATC 1 cut(s) 363
BspTNI GGTCTC 1 cut(s) 173
BsrI ACTGG 2 cut(s) 99, 189
BssHII GCGCGC 1 cut(s) 34
BssMI GATC 5 cut(s) 73, 79, 85, 135, 355
BssNI GRCGYC 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 25
Bst6I CTCTTC 4 cut(s) 23, 171, 402, 507
BstACI GRCGYC 1 cut(s) 612
BstC8I GCNNGC 3 cut(s) 36, 54, 487
BstDEI CTNAG 1 cut(s) 443
BstFNI CGCG 3 cut(s) 36, 38, 263
BstHHI GCGC 3 cut(s) 36, 38, 151
BstKTI GATC 5 cut(s) 76, 82, 88, 138, 358
BstMAI GTCTC 2 cut(s) 173, 522
BstMBI GATC 5 cut(s) 73, 79, 85, 135, 355
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 25
BstSCI CCNGG 1 cut(s) 23
BstUI CGCG 3 cut(s) 36, 38, 263
BstV2I GAAGAC 1 cut(s) 314
BstZI CGGCCG 1 cut(s) 39
BsuRI GGCC 2 cut(s) 41, 65
BtsIMutI CAGTG 2 cut(s) 106, 196
BtuMI TCGCGA 1 cut(s) 263
Cac8I GCNNGC 3 cut(s) 36, 54, 487
CfoI GCGC 3 cut(s) 36, 38, 151
CseI GACGC 1 cut(s) 601
CviAII CATG 2 cut(s) 118, 476
CviJI RGCY 9 cut(s) 17, 41, 46, 52, 65, 125, 197, 282, 485
CviKI_1 RGCY 9 cut(s) 17, 41, 46, 52, 65, 125, 197, 282, 485
DdeI CTNAG 1 cut(s) 443
DpnI GATC 5 cut(s) 75, 81, 87, 137, 357
DpnII GATC 5 cut(s) 73, 79, 85, 135, 355
EaeI YGGCCR 2 cut(s) 39, 63
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 4 cut(s) 23, 171, 402, 507
EarI CTCTTC 4 cut(s) 23, 171, 402, 507
Ecl136II GAGCTC 2 cut(s) 52, 282
EclXI CGGCCG 1 cut(s) 39
Eco24I GRGCYC 3 cut(s) 48, 54, 284
Eco31I GGTCTC 1 cut(s) 173
Eco32I GATATC 2 cut(s) 106, 468
Eco52I CGGCCG 1 cut(s) 39
Eco53kI GAGCTC 2 cut(s) 52, 282
Eco57I CTGAAG 3 cut(s) 222, 248, 582
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 2 cut(s) 52, 282
EcoRII CCWGG 1 cut(s) 23
EcoRV GATATC 2 cut(s) 106, 468
EcoT38I GRGCYC 3 cut(s) 48, 54, 284
FaeI CATG 2 cut(s) 121, 479
FaiI YATR 4 cut(s) 119, 435, 464, 477
FaqI GGGAC 1 cut(s) 599
FatI CATG 2 cut(s) 117, 475
Fnu4HI GCNGC 1 cut(s) 39
FriOI GRGCYC 3 cut(s) 48, 54, 284
Fsp4HI GCNGC 1 cut(s) 39
GlaI GCGC 3 cut(s) 35, 37, 150
GluI GCNGC 1 cut(s) 39
GsuI CTGGAG 1 cut(s) 82
HaeIII GGCC 2 cut(s) 41, 65
HgaI GACGC 1 cut(s) 601
HhaI GCGC 3 cut(s) 36, 38, 151
Hin1I GRCGYC 1 cut(s) 612
Hin1II CATG 2 cut(s) 121, 479
Hin6I GCGC 3 cut(s) 34, 36, 149
HinP1I GCGC 3 cut(s) 34, 36, 149
HindIII AAGCTT 1 cut(s) 483
HinfI GANTC 3 cut(s) 114, 162, 515
Hpy166II GTNNAC 2 cut(s) 6, 337
Hpy188I TCNGA 6 cut(s) 140, 202, 446, 472, 522, 601
Hpy188III TCNNGA 6 cut(s) 77, 83, 89, 166, 262, 584
Hpy8I GTNNAC 2 cut(s) 6, 337
HpyAV CCTTC 4 cut(s) 139, 238, 406, 602
HpyCH4V TGCA 3 cut(s) 130, 351, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 443
Hsp92I GRCGYC 1 cut(s) 612
Hsp92II CATG 2 cut(s) 121, 479
HspAI GCGC 3 cut(s) 34, 36, 149
Kzo9I GATC 5 cut(s) 73, 79, 85, 135, 355
LpnPI CCDG 9 cut(s) 10, 37, 112, 193, 202, 235, 392, 521, 569
LweI GCATC 1 cut(s) 583
MalI GATC 5 cut(s) 75, 81, 87, 137, 357
MboI GATC 5 cut(s) 73, 79, 85, 135, 355
MboII GAAGA 9 cut(s) 40, 181, 188, 319, 416, 419, 524, 541, 617
MhlI GDGCHC 3 cut(s) 48, 54, 284
MluCI AATT 3 cut(s) 298, 344, 498
MlyI GAGTC 2 cut(s) 156, 524
MnlI CCTC 4 cut(s) 24, 80, 104, 555
MspR9I CCNGG 1 cut(s) 25
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 3 cut(s) 36, 38, 263
MwoI GCNNNNNNNGC 1 cut(s) 62
NdeII GATC 5 cut(s) 73, 79, 85, 135, 355
NlaIII CATG 2 cut(s) 121, 479
NmeAIII GCCGAG 1 cut(s) 67
NruI TCGCGA 1 cut(s) 263
PauI GCGCGC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 114
PkrI GCNGC 1 cut(s) 40
PleI GAGTC 2 cut(s) 156, 523
PpsI GAGTC 2 cut(s) 156, 523
Psp124BI GAGCTC 2 cut(s) 54, 284
Psp6I CCWGG 1 cut(s) 23
PspGI CCWGG 1 cut(s) 23
PteI GCGCGC 1 cut(s) 34
RruI TCGCGA 1 cut(s) 263
SacI GAGCTC 2 cut(s) 54, 284
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 5 cut(s) 73, 79, 85, 135, 355
SchI GAGTC 2 cut(s) 156, 524
ScrFI CCNGG 1 cut(s) 25
SduI GDGCHC 3 cut(s) 48, 54, 284
SetI ASST 7 cut(s) 54, 72, 96, 212, 284, 388, 487
SfaNI GCATC 1 cut(s) 583
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 3 cut(s) 298, 344, 498
SsiI CCGC 2 cut(s) 9, 38
SstI GAGCTC 2 cut(s) 54, 284
StyD4I CCNGG 1 cut(s) 23
TaqI TCGA 6 cut(s) 72, 78, 84, 90, 233, 430
TasI AATT 3 cut(s) 298, 344, 498
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 114
TscAI CASTG 2 cut(s) 106, 196
TspDTI ATGAA 4 cut(s) 237, 492, 567, 618
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 2 cut(s) 106, 196
XapI RAATTY 2 cut(s) 344, 498
XcmI CCANNNNNNNNNTGG 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.