pycom05g29130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014040
Physical Location & Seq
Reverse (-)
29642557 .. 29644668
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29130.8

Sequence Viewer

Length: 771 bp
ATGCCAGGAACAATTTCCTTTCTAATTTATAAATTCAGCAGTTTAGTAGCTACTCAAACAGTCCCAAATTTGAAAGCGCGGGAATCTCACTCGGTGACTCAAACGACCTTCTTCAACCTCCAAGCCACAATCCTCAAGCTCCAAACCCTAACAACAATGTCGACCGCCAAAGCAAGCAACAAGAAGCGCAGGACCGACCCGGAGATCGCCAAATCGGACCACCTCGATCTCGACCTCGACCTCTCCAATGATATCAAAGGAATCATGTCAGCGATTCATCAGATCCGAGAGAAGGCTCACAAGGACGGTCTGAAGAAGAACGAAGAGACGATTTCGAGTGTGGCAGCTGAGGTCAGGTCTGCCATTGATGAGCTCAAGTCCAAACTCGAAAAGGATAGGCAAAGTTTCGCTAAATCCCTCTCGAAGAGCTCAAAAGAGTGTGACAATTGCTTGAAGACTGAAGCTGCAAAGTTCCAAGCACTTCATGAAAAGTTCTGCAAGGACCAAGCAAGCCATCTGCAGGCCTTGAAAGGAGTTATTTCGACATATGAAGAAGAGAAGGAAAGGCTCTGCACACGATACGAACAATTGAGGAAGAGAGAAAGGAGTATGATTTCTGAGCATGAGAAAGCTTGCGCTGATAAAATTAACAAACTGGAAGAGTCATTGAAAAAGAAGAAGCAGGACGACAGAACTTTCAGCGTTCTGAGAAAGTCTCTTGGTTCAATTTTGGACAATGGTTCCGACGAGGACATCCCGGCTGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

28.94

Weight (kDa)

8.79

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33793 AT2G33793 AT2G33793
fragaria_vesca FvH4_3g09320
malus_domestica MD05G1313300.v1.1
prunus_persica Prupe.4G046300_v2.0.a1
pyrus_communis pycom05g29130
rosa_chinensis RchiOBHm_Chr5g0008281
rosa_laevigata RLG00000031558
rosa_roxburghii Rroxscaffold_1G00055470 Rroxscaffold_1G00068180
rosa_rugosa Rorug04G0434700
rosa_samantha Rh5AG063400 Rh5BG059900 Rh5CG070200 Rh5DG059400
rosa_wichuraiana Rw5G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 30
AccI GTMKAC 1 cut(s) 161
AccII CGCG 1 cut(s) 79
AciI CCGC 2 cut(s) 79, 165
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 2 cut(s) 32, 67
AcuI CTGAAG 2 cut(s) 332, 480
AdeI CACNNNGTG 1 cut(s) 94
AfiI CCNNNNNNNGG 2 cut(s) 292, 520
AgsI TTSAA 6 cut(s) 73, 115, 454, 529, 670, 726
AjnI CCWGG 1 cut(s) 4
AluBI AGCT 7 cut(s) 50, 139, 347, 373, 429, 464, 632
AluI AGCT 7 cut(s) 50, 139, 347, 373, 429, 464, 632
Alw21I GWGCWC 2 cut(s) 375, 431
Alw26I GTCTC 2 cut(s) 320, 720
AlwI GGATC 1 cut(s) 277
AoxI GGCC 1 cut(s) 522
ApeKI GCWGC 2 cut(s) 344, 464
ApoI RAATTY 2 cut(s) 32, 67
Asp700I GAANNNNTTC 1 cut(s) 13
AspLEI GCGC 3 cut(s) 79, 189, 638
AspS9I GGNCC 3 cut(s) 192, 217, 502
AsuC2I CCSGG 2 cut(s) 200, 758
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 3 cut(s) 192, 217, 502
BanII GRGCYC 2 cut(s) 375, 431
BbsI GAAGAC 1 cut(s) 461
Bbv12I GWGCWC 2 cut(s) 375, 431
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 356, 451
BccI CCATC 1 cut(s) 522
BciT130I CCWGG 1 cut(s) 6
BcnI CCSGG 2 cut(s) 200, 758
BcoDI GTCTC 2 cut(s) 320, 720
BfmI CTRYAG 1 cut(s) 518
BisI GCNGC 2 cut(s) 345, 465
BlsI GCNGC 2 cut(s) 346, 466
Bme1390I CCNGG 3 cut(s) 6, 200, 758
Bme18I GGWCC 3 cut(s) 192, 217, 502
BmgT120I GGNCC 3 cut(s) 192, 217, 502
BmiI GGNNCC 1 cut(s) 742
BmrFI CCNGG 3 cut(s) 6, 200, 758
BpiI GAAGAC 1 cut(s) 461
BplI GAGNNNNNCTC 2 cut(s) 700, 732
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 2 cut(s) 119, 359
BpuMI CCSGG 2 cut(s) 200, 758
Bsc4I CCNNNNNNNGG 2 cut(s) 292, 520
Bse1I ACTGG 1 cut(s) 660
BseBI CCWGG 1 cut(s) 6
BseGI GGATG 1 cut(s) 753
BseLI CCNNNNNNNGG 2 cut(s) 292, 520
BseMII CTCAG 3 cut(s) 339, 609, 698
BseNI ACTGG 1 cut(s) 660
BseXI GCAGC 2 cut(s) 356, 451
BsgI GTGCAG 1 cut(s) 556
Bsh1236I CGCG 1 cut(s) 79
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 1 cut(s) 524
BsiEI CGRYCG 1 cut(s) 165
BsiHKAI GWGCWC 2 cut(s) 375, 431
BsiSI CCGG 2 cut(s) 200, 758
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 292, 520
BsmAI GTCTC 2 cut(s) 320, 720
BsmBI CGTCTC 1 cut(s) 320
BsmFI GGGAC 1 cut(s) 47
BsnI GGCC 1 cut(s) 524
Bsp1286I GDGCHC 2 cut(s) 375, 431
Bsp143I GATC 3 cut(s) 204, 226, 282
BspACI CCGC 2 cut(s) 79, 165
BspANI GGCC 1 cut(s) 524
BspCNI CTCAG 3 cut(s) 340, 610, 699
BspFNI CGCG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 742
BspMAI CTGCAG 1 cut(s) 522
BspPI GGATC 1 cut(s) 277
BspQI GCTCTTC 1 cut(s) 419
BsrI ACTGG 1 cut(s) 660
BssMI GATC 3 cut(s) 204, 226, 282
Bst2UI CCWGG 1 cut(s) 6
Bst4CI ACNGT 2 cut(s) 61, 308
Bst6I CTCTTC 5 cut(s) 318, 419, 549, 590, 654
BstC8I GCNNGC 4 cut(s) 175, 511, 522, 634
BstDEI CTNAG 3 cut(s) 348, 618, 707
BstF5I GGATG 1 cut(s) 753
BstFNI CGCG 1 cut(s) 79
BstHHI GCGC 3 cut(s) 79, 189, 638
BstKTI GATC 3 cut(s) 207, 229, 285
BstMAI GTCTC 2 cut(s) 320, 720
BstMBI GATC 3 cut(s) 204, 226, 282
BstMCI CGRYCG 1 cut(s) 165
BstNI CCWGG 1 cut(s) 6
BstSCI CCNGG 3 cut(s) 4, 198, 756
BstSFI CTRYAG 1 cut(s) 518
BstUI CGCG 1 cut(s) 79
BstV1I GCAGC 2 cut(s) 356, 451
BstV2I GAAGAC 1 cut(s) 461
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BsuRI GGCC 1 cut(s) 524
BtsCI GGATG 1 cut(s) 753
Cac8I GCNNGC 4 cut(s) 175, 511, 522, 634
CciI TCATGA 1 cut(s) 484
CfoI GCGC 3 cut(s) 79, 189, 638
Cfr13I GGNCC 3 cut(s) 192, 217, 502
CviAII CATG 3 cut(s) 265, 485, 623
DdeI CTNAG 3 cut(s) 348, 618, 707
DpnI GATC 3 cut(s) 206, 228, 284
DpnII GATC 3 cut(s) 204, 226, 282
DraIII CACNNNGTG 1 cut(s) 94
Eam1104I CTCTTC 5 cut(s) 318, 419, 549, 590, 654
EarI CTCTTC 5 cut(s) 318, 419, 549, 590, 654
Ecl136II GAGCTC 2 cut(s) 373, 429
Eco147I AGGCCT 1 cut(s) 524
Eco24I GRGCYC 2 cut(s) 375, 431
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 3 cut(s) 192, 217, 502
Eco53kI GAGCTC 2 cut(s) 373, 429
Eco57I CTGAAG 2 cut(s) 332, 480
EcoICRI GAGCTC 2 cut(s) 373, 429
EcoRII CCWGG 1 cut(s) 4
EcoRV GATATC 1 cut(s) 253
EcoT38I GRGCYC 2 cut(s) 375, 431
Esp3I CGTCTC 1 cut(s) 320
FaeI CATG 3 cut(s) 268, 488, 626
FaiI YATR 7 cut(s) 30, 266, 486, 547, 549, 611, 624
FaqI GGGAC 1 cut(s) 47
FatI CATG 3 cut(s) 264, 484, 622
FauI CCCGC 1 cut(s) 72
FauNDI CATATG 1 cut(s) 547
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 2 cut(s) 345, 465
FokI GGATG 1 cut(s) 740
FriOI GRGCYC 2 cut(s) 375, 431
Fsp4HI GCNGC 2 cut(s) 345, 465
GlaI GCGC 3 cut(s) 78, 188, 637
GluI GCNGC 2 cut(s) 345, 465
HaeIII GGCC 1 cut(s) 524
HapII CCGG 2 cut(s) 200, 758
HhaI GCGC 3 cut(s) 79, 189, 638
Hin1II CATG 3 cut(s) 268, 488, 626
Hin6I GCGC 3 cut(s) 77, 187, 636
HinP1I GCGC 3 cut(s) 77, 187, 636
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 5 cut(s) 83, 97, 261, 274, 662
HpaII CCGG 2 cut(s) 200, 758
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 7 cut(s) 217, 282, 287, 312, 619, 708, 745
Hpy188III TCNNGA 3 cut(s) 230, 421, 485
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 3 cut(s) 118, 286, 553
HpyCH4III ACNGT 2 cut(s) 61, 308
HpyCH4V TGCA 4 cut(s) 467, 498, 520, 573
HpyF3I CTNAG 3 cut(s) 348, 618, 707
Hsp92II CATG 3 cut(s) 268, 488, 626
HspAI GCGC 3 cut(s) 77, 187, 636
Kzo9I GATC 3 cut(s) 204, 226, 282
LguI GCTCTTC 1 cut(s) 419
LmnI GCTCC 1 cut(s) 144
LpnPI CCDG 7 cut(s) 18, 175, 213, 340, 506, 641, 668
Lsp1109I GCAGC 2 cut(s) 356, 451
MaeIII GTNAC 2 cut(s) 94, 440
MalI GATC 3 cut(s) 206, 228, 284
MboI GATC 3 cut(s) 204, 226, 282
MfeI CAATTG 2 cut(s) 445, 587
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 2 cut(s) 375, 431
MluCI AATT 8 cut(s) 12, 24, 32, 67, 445, 587, 645, 726
MlyI GAGTC 2 cut(s) 91, 671
MmeI TCCRAC 1 cut(s) 768
MnlI CCTC 9 cut(s) 128, 143, 233, 245, 251, 343, 428, 585, 742
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 1 cut(s) 648
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 2 cut(s) 200, 758
MspR9I CCNGG 3 cut(s) 6, 200, 758
MunI CAATTG 2 cut(s) 445, 587
MvaI CCWGG 1 cut(s) 6
MvnI CGCG 1 cut(s) 79
NciI CCSGG 2 cut(s) 200, 758
NdeI CATATG 1 cut(s) 547
NdeII GATC 3 cut(s) 204, 226, 282
NlaIII CATG 3 cut(s) 268, 488, 626
NlaIV GGNNCC 1 cut(s) 742
NmuCI GTSAC 2 cut(s) 94, 440
PagI TCATGA 1 cut(s) 484
PceI AGGCCT 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 419
PdmI GAANNNNTTC 1 cut(s) 13
PfeI GAWTC 3 cut(s) 83, 261, 274
PkrI GCNGC 2 cut(s) 346, 466
PleI GAGTC 2 cut(s) 91, 670
PpsI GAGTC 2 cut(s) 91, 670
PsiI TTATAA 1 cut(s) 30
Psp124BI GAGCTC 2 cut(s) 375, 431
Psp6I CCWGG 1 cut(s) 4
PspGI CCWGG 1 cut(s) 4
PspN4I GGNNCC 1 cut(s) 742
PspPI GGNCC 3 cut(s) 192, 217, 502
PstI CTGCAG 1 cut(s) 522
PsuI RGATCY 1 cut(s) 282
PvuII CAGCTG 1 cut(s) 347
SacI GAGCTC 2 cut(s) 375, 431
SalI GTCGAC 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 419
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 2 cut(s) 345, 465
Sau3AI GATC 3 cut(s) 204, 226, 282
Sau96I GGNCC 3 cut(s) 192, 217, 502
SchI GAGTC 2 cut(s) 91, 671
ScrFI CCNGG 3 cut(s) 6, 200, 758
SduI GDGCHC 2 cut(s) 375, 431
SfcI CTRYAG 1 cut(s) 518
SinI GGWCC 3 cut(s) 192, 217, 502
SmlI CTYRAG 2 cut(s) 134, 374
SmoI CTYRAG 2 cut(s) 134, 374
Sse9I AATT 8 cut(s) 12, 24, 32, 67, 445, 587, 645, 726
SseBI AGGCCT 1 cut(s) 524
SsiI CCGC 2 cut(s) 79, 165
SstI GAGCTC 2 cut(s) 375, 431
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 3 cut(s) 4, 198, 756
TaaI ACNGT 2 cut(s) 61, 308
TaqI TCGA 8 cut(s) 161, 225, 231, 237, 335, 387, 422, 542
TaqII GACCGA 1 cut(s) 209
TasI AATT 8 cut(s) 12, 24, 32, 67, 445, 587, 645, 726
TfiI GAWTC 3 cut(s) 83, 261, 274
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TseFI GTSAC 2 cut(s) 94, 440
TseI GCWGC 2 cut(s) 344, 464
Tsp45I GTSAC 2 cut(s) 94, 440
TspDTI ATGAA 4 cut(s) 266, 473, 501, 564
VpaK11BI GGWCC 3 cut(s) 192, 217, 502
XapI RAATTY 2 cut(s) 32, 67
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.