Rh5AG063400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0014040
Physical Location & Seq
Forward (+)
5343287 .. 5345547
2261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG063400.1

Sequence Viewer

Length: 597 bp
ATGTCCACCGCCAAAGCCACCACCAGGAAGAGGCGCGCGGCCGAGCCCGAGCTCGCCAAATCGGCCAACCTCGATCTCGATCTCGACCTCTCCAGTGATATCAAAGGAATCATGTCGGCTCTGCAACAGATCAGAGAGAAGGCGCACAAGGACGGACTCAAGAAGAACGAAGAGACCATTTCCAGTGTGGCTTCTGAAGTCAGGTTTGTGATTGATGAACTGAAGTCGAAAGTTGAGAAGGACAGGCAAAGTTTCGCGAAATCACTTTCAAAGAGCTCAAAAGAGTGTGAGAATTGCTTGAAGACTGAAACTACCAAGTTCCAAGCAGTTTACGAGAAATTCTGCAAGGATCAAGCGAACCATCTACAATCCCTGAAAGGTATCATTTCAAACTTTGAAGAAGAGAAGGAAAGGCTATTCACTCGATATGAGCAACTCAGAAAGAGAGAGAGAACTATGATATCAGAGCATGAAAAAGCTTGCACTGATAAAATTTCCAAGTTAGAAGAGTCTCTGAAAAAGAAGAAGCAGGTACTGTTTCTGTCAGATCTCTCTGTATATCTATCTATGTGTGTGATTTGTATAAACATGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.74

Weight (kDa)

9.02

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASY3-like PF20435 30 - 165 9.5e-07 Meiosis-specific protein ASY3-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33793 AT2G33793 AT2G33793
fragaria_vesca FvH4_3g09320
malus_domestica MD05G1313300.v1.1
prunus_persica Prupe.4G046300_v2.0.a1
pyrus_communis pycom05g29130
rosa_chinensis RchiOBHm_Chr5g0008281
rosa_laevigata RLG00000031558
rosa_roxburghii Rroxscaffold_1G00055470 Rroxscaffold_1G00068180
rosa_rugosa Rorug04G0434700
rosa_samantha Rh5AG063400 Rh5BG059900 Rh5CG070200 Rh5DG059400
rosa_wichuraiana Rw5G005750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 520
AccII CGCG 3 cut(s) 36, 38, 257
AciI CCGC 2 cut(s) 9, 38
AclWI GGATC 1 cut(s) 357
AcoI YGGCCR 2 cut(s) 39, 63
AcsI RAATTY 2 cut(s) 338, 492
AcuI CTGAAG 2 cut(s) 216, 242
AfaI GTAC 1 cut(s) 534
AfiI CCNNNNNNNGG 2 cut(s) 24, 30
AflIII ACRYGT 1 cut(s) 588
AgsI TTSAA 4 cut(s) 270, 301, 390, 398
AjnI CCWGG 1 cut(s) 23
AluBI AGCT 3 cut(s) 52, 276, 479
AluI AGCT 3 cut(s) 52, 276, 479
Alw21I GWGCWC 2 cut(s) 54, 278
Alw26I GTCTC 2 cut(s) 167, 516
AlwI GGATC 1 cut(s) 357
AlwNI CAGNNNCTG 1 cut(s) 535
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 2 cut(s) 39, 63
ApoI RAATTY 2 cut(s) 338, 492
AspLEI GCGC 3 cut(s) 36, 38, 145
AvaI CYCGRG 1 cut(s) 47
BanII GRGCYC 3 cut(s) 48, 54, 278
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 2 cut(s) 54, 278
BccI CCATC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 25
BcoDI GTCTC 2 cut(s) 167, 516
BfuAI ACCTGC 1 cut(s) 520
BglI GCCNNNNNGGC 1 cut(s) 62
BglII AGATCT 1 cut(s) 547
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 47
BmrFI CCNGG 1 cut(s) 25
BpiI GAAGAC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 76
BpuEI CTTGAG 1 cut(s) 143
BsaBI GATNNNNATC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 30
Bse1I ACTGG 2 cut(s) 93, 183
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 1 cut(s) 25
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 2 cut(s) 24, 30
BseMII CTCAG 1 cut(s) 451
BseNI ACTGG 2 cut(s) 93, 183
BsePI GCGCGC 1 cut(s) 34
BseX3I CGGCCG 1 cut(s) 39
Bsh1236I CGCG 3 cut(s) 36, 38, 257
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 2 cut(s) 41, 65
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 2 cut(s) 54, 278
BsiHKCI CYCGRG 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 24, 30
BsmAI GTCTC 2 cut(s) 167, 516
BsnI GGCC 2 cut(s) 41, 65
Bso31I GGTCTC 1 cut(s) 167
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 3 cut(s) 48, 54, 278
Bsp143I GATC 5 cut(s) 73, 79, 129, 349, 547
Bsp68I TCGCGA 1 cut(s) 257
BspACI CCGC 2 cut(s) 9, 38
BspANI GGCC 2 cut(s) 41, 65
BspCNI CTCAG 1 cut(s) 450
BspFNI CGCG 3 cut(s) 36, 38, 257
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 1 cut(s) 357
BspTNI GGTCTC 1 cut(s) 167
BsrI ACTGG 2 cut(s) 93, 183
BssHII GCGCGC 1 cut(s) 34
BssMI GATC 5 cut(s) 73, 79, 129, 349, 547
Bst2UI CCWGG 1 cut(s) 25
Bst4CI ACNGT 1 cut(s) 537
Bst6I CTCTTC 4 cut(s) 23, 165, 396, 501
BstC8I GCNNGC 3 cut(s) 36, 54, 481
BstDEI CTNAG 1 cut(s) 437
BstFNI CGCG 3 cut(s) 36, 38, 257
BstHHI GCGC 3 cut(s) 36, 38, 145
BstKTI GATC 5 cut(s) 76, 82, 132, 352, 550
BstMAI GTCTC 2 cut(s) 167, 516
BstMBI GATC 5 cut(s) 73, 79, 129, 349, 547
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 25
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 1 cut(s) 23
BstUI CGCG 3 cut(s) 36, 38, 257
BstV2I GAAGAC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
BstZI CGGCCG 1 cut(s) 39
BsuRI GGCC 2 cut(s) 41, 65
BtsIMutI CAGTG 3 cut(s) 100, 190, 483
BtuMI TCGCGA 1 cut(s) 257
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 3 cut(s) 36, 54, 481
CaiI CAGNNNCTG 1 cut(s) 535
CfoI GCGC 3 cut(s) 36, 38, 145
Csp6I GTAC 1 cut(s) 533
CviAII CATG 3 cut(s) 112, 470, 589
CviQI GTAC 1 cut(s) 533
DdeI CTNAG 1 cut(s) 437
DpnI GATC 5 cut(s) 75, 81, 131, 351, 549
DpnII GATC 5 cut(s) 73, 79, 129, 349, 547
EaeI YGGCCR 2 cut(s) 39, 63
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 4 cut(s) 23, 165, 396, 501
EarI CTCTTC 4 cut(s) 23, 165, 396, 501
Ecl136II GAGCTC 2 cut(s) 52, 276
EclXI CGGCCG 1 cut(s) 39
Eco24I GRGCYC 3 cut(s) 48, 54, 278
Eco31I GGTCTC 1 cut(s) 167
Eco32I GATATC 2 cut(s) 100, 462
Eco52I CGGCCG 1 cut(s) 39
Eco53kI GAGCTC 2 cut(s) 52, 276
Eco57I CTGAAG 2 cut(s) 216, 242
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 2 cut(s) 52, 276
EcoRII CCWGG 1 cut(s) 23
EcoRV GATATC 2 cut(s) 100, 462
EcoT38I GRGCYC 3 cut(s) 48, 54, 278
FaeI CATG 3 cut(s) 115, 473, 592
FaiI YATR 8 cut(s) 113, 429, 458, 471, 559, 569, 584, 590
FatI CATG 3 cut(s) 111, 469, 588
Fnu4HI GCNGC 1 cut(s) 39
FriOI GRGCYC 3 cut(s) 48, 54, 278
Fsp4HI GCNGC 1 cut(s) 39
GlaI GCGC 3 cut(s) 35, 37, 144
GluI GCNGC 1 cut(s) 39
GsuI CTGGAG 1 cut(s) 76
HaeIII GGCC 2 cut(s) 41, 65
HhaI GCGC 3 cut(s) 36, 38, 145
Hin1II CATG 3 cut(s) 115, 473, 592
Hin6I GCGC 3 cut(s) 34, 36, 143
HinP1I GCGC 3 cut(s) 34, 36, 143
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 3 cut(s) 108, 156, 509
Hpy166II GTNNAC 2 cut(s) 6, 331
Hpy188I TCNGA 6 cut(s) 134, 196, 440, 466, 516, 547
Hpy188III TCNNGA 5 cut(s) 77, 83, 160, 256, 594
Hpy8I GTNNAC 2 cut(s) 6, 331
HpyAV CCTTC 3 cut(s) 133, 232, 400
HpyCH4III ACNGT 1 cut(s) 537
HpyCH4V TGCA 3 cut(s) 124, 345, 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 437
Hsp92II CATG 3 cut(s) 115, 473, 592
HspAI GCGC 3 cut(s) 34, 36, 143
Kzo9I GATC 5 cut(s) 73, 79, 129, 349, 547
LpnPI CCDG 8 cut(s) 10, 37, 106, 187, 196, 229, 386, 515
MalI GATC 5 cut(s) 75, 81, 131, 351, 549
MboI GATC 5 cut(s) 73, 79, 129, 349, 547
MboII GAAGA 8 cut(s) 40, 175, 182, 313, 410, 413, 518, 535
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 3 cut(s) 48, 54, 278
MluCI AATT 3 cut(s) 292, 338, 492
MlyI GAGTC 2 cut(s) 150, 518
MnlI CCTC 3 cut(s) 24, 80, 98
MspR9I CCNGG 1 cut(s) 25
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 3 cut(s) 36, 38, 257
MwoI GCNNNNNNNGC 1 cut(s) 62
NdeII GATC 5 cut(s) 73, 79, 129, 349, 547
NlaIII CATG 3 cut(s) 115, 473, 592
NmeAIII GCCGAG 1 cut(s) 67
NruI TCGCGA 1 cut(s) 257
NspI RCATGY 1 cut(s) 592
PauI GCGCGC 1 cut(s) 34
PciI ACATGT 1 cut(s) 588
PfeI GAWTC 1 cut(s) 108
PkrI GCNGC 1 cut(s) 40
PleI GAGTC 2 cut(s) 150, 517
PpsI GAGTC 2 cut(s) 150, 517
PscI ACATGT 1 cut(s) 588
Psp124BI GAGCTC 2 cut(s) 54, 278
Psp6I CCWGG 1 cut(s) 23
PspGI CCWGG 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 535
PsuI RGATCY 1 cut(s) 547
PteI GCGCGC 1 cut(s) 34
RruI TCGCGA 1 cut(s) 257
RsaI GTAC 1 cut(s) 534
RsaNI GTAC 1 cut(s) 533
SacI GAGCTC 2 cut(s) 54, 278
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 5 cut(s) 73, 79, 129, 349, 547
SchI GAGTC 2 cut(s) 150, 518
ScrFI CCNGG 1 cut(s) 25
SduI GDGCHC 3 cut(s) 48, 54, 278
SetI ASST 8 cut(s) 54, 72, 90, 206, 278, 382, 481, 534
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
Sse9I AATT 3 cut(s) 292, 338, 492
SsiI CCGC 2 cut(s) 9, 38
SstI GAGCTC 2 cut(s) 54, 278
StyD4I CCNGG 1 cut(s) 23
TaaI ACNGT 1 cut(s) 537
TaqI TCGA 5 cut(s) 72, 78, 84, 227, 424
TasI AATT 3 cut(s) 292, 338, 492
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 108
TscAI CASTG 3 cut(s) 100, 190, 490
TspDTI ATGAA 2 cut(s) 231, 486
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 3 cut(s) 100, 190, 490
XapI RAATTY 2 cut(s) 338, 492
XceI RCATGY 1 cut(s) 592
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.