MD05G1315100.v1.1

U2 snRNP auxiliary factor large subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008127
Physical Location & Seq
Forward (+)
44476215 .. 44482009
5795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1315100.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGCCGAGACGGAGCTTAGTTAGACGTGATGGTAATCACATACACCAGATCAAAAGAGCCTGCAAATCGCGAGCTTCTGGTTTCAGCGAATTTACATATTTAGGGTTCTTCTCATTTGAAATTCGGACTGCTACAATGTCAGATTTCGAAGGCGGCAGCAAGAATTCTCGTGGATCAGAACGTGAGAGCTCGTGGAGAGATCGAGAGCGAGACCGAGGTCAAGATAGAGAAAAAGATGGTGGCCGGTATACACGGGAGTATAAGGATAGAAGTGGAAGATTTGACAGAGGGAGGAACTCGTACAATGGCTATCGCCGCAGTCGAAACGAAGATTTTGACAGGCATCGTGATTATGATCAAGATAGAGAAAGGAGGCATAGACATAGATCACGTTCTCGTTCTCCCTCAAGTGACAGATCTCCTTCCCGTTCTCGTTCAAAGAGCAAGCGTACAAGTGGCTTTGACATGGCACCACCTGCTGGGTCAGTGCTGCCTAATGCTCCCGTTTCAGGTATTCCGCAAATGATGCCAGGAGTAGTACAAAATTCATTACCTTTTGGGGCATCGCATATGGCTCTTCCTTTGATGCCAGCCCAAGCCATGACTCAGCAGGCTACAAGGCATGCACGTCGGGTATATGTTGGTGGGCTTCCTCCCTTGGCTAATGAGCAGACAATTGCCACATTCTTTAGCCAAGTCATGGCTGCCATTGGAGGAAATTCTGTTGGTCCAGTATCTGAACTTGCAGGTGATGCGGTAGTAAATGTCTATATTAATCATGAGAAGAAGTTTGCATTTGTGGAGATGAGAACGGTTGAAGAAGCAAGTAATGCAATGGCATTAGATGGAATTATATTTGAGGGGGTTGCTGTGAGGGTACGAAGGCCAACAGACTACAATCCTACTTTAGCTGCAGCGCTTGGTCCTAGCCAACCAAGTCCTCACCTTAACTTAGCTGCCGTTGGTCTCACACAAGGTGCTGTTGGTGGAGCTGAGGGACCTGACCGTATCTTTGTGGGTGGGCTACCTTACTACTTCACTGAAGCTCAGATTAGAGAGTTGCTACAGTCCTTTGGACCTCTCCGTGGTTTTGACCTCGTAAAGGATAAAGACACAGGAAACTCTAAAGGATATGGATTCTGTGTTTATCAGGATCCAACTGTGACAGACATTGCTTGCGGTGCTCTCAACGGCTTAAAAATGGGTGATAAAACTTTAACTGTCCGGCGTGCTACTGCCAGCAACGGGCAGTCTAAAACAGAGCAAGAAAATATCTTGGTACAGGCACAACAGCATATAGCCATGCAGAAAATGGCCTTGCAGGTTGGTGACTTCAATCTTCTAGGAGCTGGAATGGCAACAATGGCTAGTGGTGAAACTCCCACAAAAGTCCTATGCTTGACTGAGGCGATTACTGCTGATCAACTAGGTGATGATGAAGAGTATGTCGAAATACTAGAAGACATGAGGGATGAATGCAGCAAATTTGGAACTCTGGTGAATGTTGTTATTCCTCGTGCGGATCAAAACGGAGAACAGATTCCAGGCGTTGGAAAGGTATTCTTGGAATATACTGACACTGGGGGTTGCGCCAACGCAAAGAATGTACTCGGTGGGAGAAAATTCGGAGGTAACATAGTGCATGCCGTTTACTATCCAGAAGAGAAATACTACAACAGGGACTACAGTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

565

Amino Acids

62.17

Weight (kDa)

8.92

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 337 - 406 2.8e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 484, 737
Acc36I ACCTGC 3 cut(s) 484, 737, 1312
AccB1I GGYRCC 1 cut(s) 469
AccB7I CCANNNNNTGG 3 cut(s) 479, 700, 1550
AccI GTMKAC 1 cut(s) 248
AccII CGCG 1 cut(s) 70
AciI CCGC 6 cut(s) 153, 316, 518, 755, 1179, 1520
AclWI GGATC 4 cut(s) 181, 1148, 1161, 1530
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 7 cut(s) 89, 120, 163, 544, 718, 1484, 1622
AcuI CTGAAG 1 cut(s) 1062
AdeI CACNNNGTG 1 cut(s) 977
AfaI GTAC 6 cut(s) 302, 451, 540, 879, 1281, 1608
AfeI AGCGCT 1 cut(s) 918
AfiI CCNNNNNNNGG 7 cut(s) 479, 509, 700, 1085, 1102, 1245, 1550
AgsI TTSAA 4 cut(s) 119, 438, 818, 1336
AjiI CACGTC 2 cut(s) 26, 629
AjnI CCWGG 2 cut(s) 529, 1543
AluBI AGCT 8 cut(s) 15, 74, 189, 911, 956, 992, 1046, 1349
AluI AGCT 8 cut(s) 15, 74, 189, 911, 956, 992, 1046, 1349
Alw21I GWGCWC 2 cut(s) 191, 1186
Alw26I GTCTC 2 cut(s) 204, 971
AlwI GGATC 4 cut(s) 181, 1148, 1161, 1530
AlwNI CAGNNNCTG 1 cut(s) 737
Aor51HI AGCGCT 1 cut(s) 918
AoxI GGCC 3 cut(s) 241, 884, 1314
ApeKI GCWGC 7 cut(s) 156, 490, 704, 911, 914, 956, 1479
ApoI RAATTY 7 cut(s) 89, 120, 163, 544, 718, 1484, 1622
ArsI GACNNNNNNTTYG 2 cut(s) 995, 1027
AseI ATTAAT 1 cut(s) 774
Asp700I GAANNNNTTC 1 cut(s) 1539
AspLEI GCGC 2 cut(s) 919, 1592
AspS9I GGNCC 4 cut(s) 728, 923, 998, 1076
AsuHPI GGTGA 7 cut(s) 761, 935, 1217, 1340, 1385, 1442, 1510
AsuII TTCGAA 1 cut(s) 147
AvaII GGWCC 4 cut(s) 728, 923, 998, 1076
BamHI GGATCC 1 cut(s) 1153
BanI GGYRCC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 191
BauI CACGAG 3 cut(s) 168, 190, 1515
BbsI GAAGAC 1 cut(s) 1467
Bbv12I GWGCWC 2 cut(s) 191, 1186
BbvCI CCTCAGC 1 cut(s) 993
BbvI GCAGC 7 cut(s) 168, 477, 691, 898, 926, 943, 1491
BccI CCATC 3 cut(s) 23, 230, 839
BceAI ACGGC 3 cut(s) 944, 1207, 1631
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 2 cut(s) 531, 1545
BclI TGATCA 2 cut(s) 355, 1420
BcoDI GTCTC 2 cut(s) 204, 971
BfaI CTAG 5 cut(s) 927, 1343, 1368, 1427, 1457
BfmI CTRYAG 3 cut(s) 912, 1064, 1684
BfoI RGCGCY 1 cut(s) 920
BfuAI ACCTGC 3 cut(s) 484, 737, 1312
BglII AGATCT 1 cut(s) 416
BisI GCNGC 9 cut(s) 154, 157, 316, 491, 705, 912, 915, 957, 1480
BlsI GCNGC 9 cut(s) 155, 158, 317, 492, 706, 913, 916, 958, 1481
Bme1390I CCNGG 2 cut(s) 531, 1545
Bme18I GGWCC 4 cut(s) 728, 923, 998, 1076
BmgBI CACGTC 2 cut(s) 26, 629
BmgT120I GGNCC 4 cut(s) 728, 923, 998, 1076
BmiI GGNNCC 3 cut(s) 471, 999, 1155
BmrFI CCNGG 2 cut(s) 531, 1545
BmrI ACTGGG 1 cut(s) 1590
BmsI GCATC 5 cut(s) 352, 516, 572, 576, 742
BmuI ACTGGG 1 cut(s) 1590
BoxI GACNNNNGTC 1 cut(s) 216
BpiI GAAGAC 1 cut(s) 1467
Bpu10I CCTNAGC 1 cut(s) 993
Bpu14I TTCGAA 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 391
BsaBI GATNNNNATC 2 cut(s) 33, 354
BsaI GGTCTC 2 cut(s) 204, 971
BsaJI CCNNGG 3 cut(s) 214, 657, 1084
Bsc4I CCNNNNNNNGG 7 cut(s) 479, 509, 700, 1085, 1102, 1245, 1550
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 2 cut(s) 731, 1585
Bse3DI GCAATG 2 cut(s) 840, 1170
Bse8I GATNNNNATC 2 cut(s) 33, 354
BseBI CCWGG 2 cut(s) 531, 1545
BseDI CCNNGG 3 cut(s) 214, 657, 1084
BseGI GGATG 1 cut(s) 1477
BseJI GATNNNNATC 2 cut(s) 33, 354
BseLI CCNNNNNNNGG 7 cut(s) 479, 509, 700, 1085, 1102, 1245, 1550
BseMI GCAATG 2 cut(s) 840, 1170
BseMII CTCAG 4 cut(s) 620, 984, 1061, 1395
BseNI ACTGG 2 cut(s) 731, 1585
BseXI GCAGC 7 cut(s) 168, 477, 691, 898, 926, 943, 1491
BseYI CCCAGC 1 cut(s) 479
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 3 cut(s) 243, 886, 1316
BshNI GGYRCC 1 cut(s) 469
BsiHKAI GWGCWC 2 cut(s) 191, 1186
BsiSI CCGG 2 cut(s) 244, 1225
BslFI GGGAC 1 cut(s) 1011
BslI CCNNNNNNNGG 7 cut(s) 479, 509, 700, 1085, 1102, 1245, 1550
BsmAI GTCTC 2 cut(s) 204, 971
BsmFI GGGAC 1 cut(s) 1011
BsmI GAATGC 1 cut(s) 1481
BsnI GGCC 3 cut(s) 243, 886, 1316
Bso31I GGTCTC 2 cut(s) 204, 971
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 191, 1186
Bsp143I GATC 9 cut(s) 48, 173, 199, 355, 386, 416, 1153, 1420, 1522
Bsp68I TCGCGA 1 cut(s) 70
BspACI CCGC 6 cut(s) 153, 316, 518, 755, 1179, 1520
BspANI GGCC 3 cut(s) 243, 886, 1316
BspCNI CTCAG 4 cut(s) 619, 985, 1060, 1396
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 3 cut(s) 471, 999, 1155
BspMAI CTGCAG 1 cut(s) 916
BspMI ACCTGC 3 cut(s) 484, 737, 1312
BspPI GGATC 4 cut(s) 181, 1148, 1161, 1530
BspQI GCTCTTC 1 cut(s) 582
BspT104I TTCGAA 1 cut(s) 147
BspT107I GGYRCC 1 cut(s) 469
BspTNI GGTCTC 2 cut(s) 204, 971
BsrDI GCAATG 2 cut(s) 840, 1170
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 2 cut(s) 731, 1585
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 3 cut(s) 214, 657, 1084
BssMI GATC 9 cut(s) 48, 173, 199, 355, 386, 416, 1153, 1420, 1522
BssNAI GTATAC 1 cut(s) 249
BssSI CACGAG 3 cut(s) 168, 190, 1515
BssT1I CCWWGG 1 cut(s) 657
Bst1107I GTATAC 1 cut(s) 249
Bst2BI CACGAG 3 cut(s) 168, 190, 1515
Bst2UI CCWGG 2 cut(s) 531, 1545
Bst4CI ACNGT 6 cut(s) 814, 1007, 1068, 1162, 1222, 1688
Bst6I CTCTTC 3 cut(s) 582, 1434, 1656
BstAPI GCANNNNNTGC 4 cut(s) 476, 526, 752, 830
BstBI TTCGAA 1 cut(s) 147
BstDEI CTNAG 6 cut(s) 16, 606, 952, 993, 1047, 1404
BstDSI CCRYGG 1 cut(s) 1084
BstENI CCTNNNNNAGG 1 cut(s) 1100
BstF5I GGATG 1 cut(s) 1477
BstFNI CGCG 1 cut(s) 70
BstH2I RGCGCY 1 cut(s) 920
BstHHI GCGC 2 cut(s) 919, 1592
BstKTI GATC 9 cut(s) 51, 176, 202, 358, 389, 419, 1156, 1423, 1525
BstMAI GTCTC 2 cut(s) 204, 971
BstMBI GATC 9 cut(s) 48, 173, 199, 355, 386, 416, 1153, 1420, 1522
BstMWI GCNNNNNNNGC 9 cut(s) 315, 476, 526, 752, 830, 1181, 1355, 1364, 1415
BstNI CCWGG 2 cut(s) 531, 1545
BstNSI RCATGY 2 cut(s) 626, 1646
BstPAI GACNNNNGTC 1 cut(s) 216
BstSCI CCNGG 2 cut(s) 529, 1543
BstSFI CTRYAG 3 cut(s) 912, 1064, 1684
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 7 cut(s) 168, 477, 691, 898, 926, 943, 1491
BstV2I GAAGAC 1 cut(s) 1467
BstX2I RGATCY 2 cut(s) 416, 1153
BstYI RGATCY 2 cut(s) 416, 1153
BstZ17I GTATAC 1 cut(s) 249
BsuRI GGCC 3 cut(s) 243, 886, 1316
BtgI CCRYGG 1 cut(s) 1084
BtgZI GCGATG 1 cut(s) 549
BtrI CACGTC 2 cut(s) 26, 629
BtsCI GGATG 1 cut(s) 1477
BtsIMutI CAGTG 4 cut(s) 492, 1038, 1578, 1693
BtuMI TCGCGA 1 cut(s) 70
BveI ACCTGC 3 cut(s) 484, 737, 1312
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 778
CfoI GCGC 2 cut(s) 919, 1592
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 4 cut(s) 728, 923, 998, 1076
Csp6I GTAC 6 cut(s) 301, 450, 539, 878, 1280, 1607
CviAII CATG 8 cut(s) 466, 601, 623, 700, 779, 1303, 1465, 1643
CviQI GTAC 6 cut(s) 301, 450, 539, 878, 1280, 1607
DdeI CTNAG 6 cut(s) 16, 606, 952, 993, 1047, 1404
DpnI GATC 9 cut(s) 50, 175, 201, 357, 388, 418, 1155, 1422, 1524
DpnII GATC 9 cut(s) 48, 173, 199, 355, 386, 416, 1153, 1420, 1522
DraIII CACNNNGTG 1 cut(s) 977
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 582, 1434, 1656
EarI CTCTTC 3 cut(s) 582, 1434, 1656
Ecl136II GAGCTC 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 657
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 2 cut(s) 204, 971
Eco47I GGWCC 4 cut(s) 728, 923, 998, 1076
Eco47III AGCGCT 1 cut(s) 918
Eco53kI GAGCTC 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 1062
EcoICRI GAGCTC 1 cut(s) 189
EcoNI CCTNNNNNAGG 1 cut(s) 1100
EcoO109I RGGNCCY 1 cut(s) 998
EcoRI GAATTC 1 cut(s) 163
EcoRII CCWGG 2 cut(s) 529, 1543
EcoT14I CCWWGG 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 8 cut(s) 469, 604, 626, 703, 782, 1306, 1468, 1646
FalI AAGNNNNNCTT 2 cut(s) 1547, 1579
FaqI GGGAC 1 cut(s) 1011
FatI CATG 8 cut(s) 465, 600, 622, 699, 778, 1302, 1464, 1642
FauNDI CATATG 1 cut(s) 570
FbaI TGATCA 2 cut(s) 355, 1420
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 9 cut(s) 154, 157, 316, 491, 705, 912, 915, 957, 1480
FokI GGATG 1 cut(s) 1484
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 9 cut(s) 154, 157, 316, 491, 705, 912, 915, 957, 1480
FspBI CTAG 5 cut(s) 927, 1343, 1368, 1427, 1457
GlaI GCGC 2 cut(s) 918, 1591
GluI GCNGC 9 cut(s) 154, 157, 316, 491, 705, 912, 915, 957, 1480
GsaI CCCAGC 1 cut(s) 483
HaeII RGCGCY 1 cut(s) 920
HaeIII GGCC 3 cut(s) 243, 886, 1316
HapII CCGG 2 cut(s) 244, 1225
HhaI GCGC 2 cut(s) 919, 1592
Hin1II CATG 8 cut(s) 469, 604, 626, 703, 782, 1306, 1468, 1646
Hin6I GCGC 2 cut(s) 917, 1590
HinP1I GCGC 2 cut(s) 917, 1590
HinfI GANTC 3 cut(s) 604, 1137, 1540
HpaII CCGG 2 cut(s) 244, 1225
HphI GGTGA 7 cut(s) 761, 935, 1217, 1340, 1385, 1442, 1510
Hpy166II GTNNAC 2 cut(s) 249, 1651
Hpy188I TCNGA 6 cut(s) 126, 142, 178, 739, 1050, 1628
Hpy188III TCNNGA 8 cut(s) 69, 203, 221, 347, 359, 779, 1151, 1658
Hpy8I GTNNAC 2 cut(s) 249, 1651
Hpy99I CGWCG 1 cut(s) 633
HpyAV CCTTC 3 cut(s) 143, 432, 876
HpyCH4III ACNGT 6 cut(s) 814, 1007, 1068, 1162, 1222, 1688
HpyCH4IV ACGT 4 cut(s) 25, 181, 391, 628
HpyF10VI GCNNNNNNNGC 9 cut(s) 315, 476, 526, 752, 830, 1181, 1355, 1364, 1415
HpyF3I CTNAG 6 cut(s) 16, 606, 952, 993, 1047, 1404
HpySE526I ACGT 4 cut(s) 25, 181, 391, 628
Hsp92II CATG 8 cut(s) 469, 604, 626, 703, 782, 1306, 1468, 1646
HspAI GCGC 2 cut(s) 917, 1590
Ksp22I TGATCA 2 cut(s) 355, 1420
Kzo9I GATC 9 cut(s) 48, 173, 199, 355, 386, 416, 1153, 1420, 1522
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 4 cut(s) 12, 505, 989, 1346
Lsp1109I GCAGC 7 cut(s) 168, 477, 691, 898, 926, 943, 1491
LweI GCATC 5 cut(s) 352, 516, 572, 576, 742
MaeI CTAG 5 cut(s) 927, 1343, 1368, 1427, 1457
MaeII ACGT 4 cut(s) 25, 181, 391, 628
MaeIII GTNAC 4 cut(s) 410, 1162, 1328, 1631
MalI GATC 9 cut(s) 50, 175, 201, 357, 388, 418, 1155, 1422, 1524
MboI GATC 9 cut(s) 48, 173, 199, 355, 386, 416, 1153, 1420, 1522
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 2 cut(s) 416, 1153
MhlI GDGCHC 2 cut(s) 191, 1186
MluCI AATT 9 cut(s) 89, 120, 163, 544, 675, 718, 849, 1484, 1622
MlyI GAGTC 1 cut(s) 598
MmeI TCCRAC 2 cut(s) 1181, 1531
MroXI GAANNNNTTC 1 cut(s) 1539
MseI TTAA 4 cut(s) 774, 948, 1196, 1217
MspI CCGG 2 cut(s) 244, 1225
MspR9I CCNGG 2 cut(s) 531, 1545
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 1481
MvaI CCWGG 2 cut(s) 531, 1545
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 9 cut(s) 315, 476, 526, 752, 830, 1181, 1355, 1364, 1415
NdeI CATATG 1 cut(s) 570
NdeII GATC 9 cut(s) 48, 173, 199, 355, 386, 416, 1153, 1420, 1522
NlaIII CATG 8 cut(s) 469, 604, 626, 703, 782, 1306, 1468, 1646
NlaIV GGNNCC 3 cut(s) 471, 999, 1155
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 3 cut(s) 410, 1162, 1328
NruI TCGCGA 1 cut(s) 70
NspI RCATGY 2 cut(s) 626, 1646
NspV TTCGAA 1 cut(s) 147
PaeI GCATGC 2 cut(s) 626, 1646
PagI TCATGA 1 cut(s) 778
PaqCI CACCTGC 2 cut(s) 484, 737
PciSI GCTCTTC 1 cut(s) 582
PcsI WCGNNNNNNNCGW 1 cut(s) 319
PctI GAATGC 1 cut(s) 1481
PdmI GAANNNNTTC 1 cut(s) 1539
PfeI GAWTC 2 cut(s) 1137, 1540
PflMI CCANNNNNTGG 3 cut(s) 479, 700, 1550
PkrI GCNGC 9 cut(s) 155, 158, 317, 492, 706, 913, 916, 958, 1481
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
PpuMI RGGWCCY 1 cut(s) 998
PshAI GACNNNNGTC 1 cut(s) 216
PshBI ATTAAT 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 998
Psp6I CCWGG 2 cut(s) 529, 1543
PspFI CCCAGC 1 cut(s) 479
PspGI CCWGG 2 cut(s) 529, 1543
PspN4I GGNNCC 3 cut(s) 471, 999, 1155
PspPI GGNCC 4 cut(s) 728, 923, 998, 1076
PspPPI RGGWCCY 1 cut(s) 998
PstI CTGCAG 1 cut(s) 916
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 2 cut(s) 416, 1153
RruI TCGCGA 1 cut(s) 70
RsaI GTAC 6 cut(s) 302, 451, 540, 879, 1281, 1608
RsaNI GTAC 6 cut(s) 301, 450, 539, 878, 1280, 1607
SacI GAGCTC 1 cut(s) 191
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 4 cut(s) 774, 948, 1196, 1217
SatI GCNGC 9 cut(s) 154, 157, 316, 491, 705, 912, 915, 957, 1480
Sau3AI GATC 9 cut(s) 48, 173, 199, 355, 386, 416, 1153, 1420, 1522
Sau96I GGNCC 4 cut(s) 728, 923, 998, 1076
SchI GAGTC 1 cut(s) 598
ScrFI CCNGG 2 cut(s) 531, 1545
SduI GDGCHC 2 cut(s) 191, 1186
SfaNI GCATC 5 cut(s) 352, 516, 572, 576, 742
SfcI CTRYAG 3 cut(s) 912, 1064, 1684
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 4 cut(s) 728, 923, 998, 1076
SmlI CTYRAG 1 cut(s) 406
SmoI CTYRAG 1 cut(s) 406
SphI GCATGC 2 cut(s) 626, 1646
Sse9I AATT 9 cut(s) 89, 120, 163, 544, 675, 718, 849, 1484, 1622
SsiI CCGC 6 cut(s) 153, 316, 518, 755, 1179, 1520
SspMI CTAG 5 cut(s) 927, 1343, 1368, 1427, 1457
SstI GAGCTC 1 cut(s) 191
StyD4I CCNGG 2 cut(s) 529, 1543
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 6 cut(s) 814, 1007, 1068, 1162, 1222, 1688
TaiI ACGT 4 cut(s) 28, 184, 394, 631
TaqI TCGA 4 cut(s) 147, 202, 322, 1449
TaqII GACCGA 1 cut(s) 228
TasI AATT 9 cut(s) 89, 120, 163, 544, 675, 718, 849, 1484, 1622
TatI WGTACW 2 cut(s) 538, 1606
TauI GCSGC 2 cut(s) 156, 318
TfiI GAWTC 2 cut(s) 1137, 1540
Tru1I TTAA 4 cut(s) 774, 948, 1196, 1217
Tru9I TTAA 4 cut(s) 774, 948, 1196, 1217
TscAI CASTG 4 cut(s) 492, 1045, 1585, 1693
TseFI GTSAC 3 cut(s) 410, 1162, 1328
TseI GCWGC 7 cut(s) 156, 490, 704, 911, 914, 956, 1479
Tsp45I GTSAC 3 cut(s) 410, 1162, 1328
TspDTI ATGAA 3 cut(s) 537, 1452, 1488
TspGWI ACGGA 3 cut(s) 25, 1073, 1545
TspRI CASTG 4 cut(s) 492, 1045, 1585, 1693
Van91I CCANNNNNTGG 3 cut(s) 479, 700, 1550
VpaK11BI GGWCC 4 cut(s) 728, 923, 998, 1076
VspI ATTAAT 1 cut(s) 774
XagI CCTNNNNNAGG 1 cut(s) 1100
XapI RAATTY 7 cut(s) 89, 120, 163, 544, 718, 1484, 1622
XceI RCATGY 2 cut(s) 626, 1646
XcmI CCANNNNNNNNNTGG 1 cut(s) 1309
XmiI GTMKAC 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 1539
XspI CTAG 5 cut(s) 927, 1343, 1368, 1427, 1457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.