MD05G1316500.v1.1

RNA polymerase II C-terminal domain phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007472
Physical Location & Seq
Reverse (-)
44562018 .. 44570133
8116 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1316500.v1.1.491

Sequence Viewer

Length: 2883 bp
ATGTATAAGTTCGTGTATAAAGGCGAAGATTTACTTGGGGAAGTAGAGATATATCCAACAGTGAACGAGAACAACAAGAATGTTCAGGATGTGTTGAAGGAAATCAGAATAAGCTATTTTTCTCAACCAAGTGAAAGATGTCCACCAGTGGCAGTGCTTCATACCATAAACTCCTCCAATGGAGTTTGCTTCAAAATGATGGAGTCAAAGACGTCGCCGTTGTCGTCTCCGGACACGCCGCTCTTTCTTTTGCACTCCTCCATGACTCAGGAGAACAAGACTGCAGTAATGCCCCTAGGAGGGGAGGAGCTTCATTTGGTTGCCATGCAATCCAGGAATGGTGGTAAACAGTTCCCATGTTTCTGGGGATTCTATGTTGCATCAGGACTATACAATTCTTGCCTTGTCATGCTGAATCTTAGATGTCTGGGCATTGTATTTGATCTTGATGAAACACTCATTGTTGCAAATACAATGCGCTCTTTTGAGGATCGAATTGAGGCCCTACAGAGGAAGATAAGCACTGAGGTGGACCCACTACGTATTTCTGGTATGCTTGCAGAGATCAAGCGGTATCAAGATGACAAGTTTATATTGAAGCAATATGCTGAAAATGATCAAGTTGTTGATAATGGGAGAGTTGTCAAAACTCAATCGGAGGTTGTTCCAGCCTTGTCTGACAATCATCAACCTATTATTCGGCCTCTTATACGATTACATGAGAAAAATATTATACTGACTCGCATTAATCCGCAGATTCGTGATACAAGTGTTCTTGTGAGGTTGAGACCTGCATGGGAAGATCTTCGGAGCTACCTGACTGCAAGAGGGCGCAAGCGGTTTGAGGTTTATGTTTGCACAATGGCTGAAAGGGATTATGCATTGGAAATGTGGAGGCTTCTTGATCCTGATTCAAACTTGATAAACTCAAATAAATTACTTGATCGTATTGTCTGTGTGAAGTCTGGTTCAAGAAAGTCGTTGTTCAATGTCTTCCAAGAAAGCTTATGCCATCCTAAGATGGCATTGGTAATTGATGATCGCTTGAAAGTGTGGGATGAGAGAGATCAACCTCGGGTGCATGTTGTTCCTGCTTTTGCTCCGTATTATGCTCCTCAAGCTGAAGCAAATAACACAGTCCCTGTCCTGTGTGTTGCAAGAAATGTTGCTTGCAATGTCAGAGGTGGATTTTTTAAAGAATTTGATGACAGTCTTCTACAAAAGATTCCTGAATTTTTTTATGAAGATGATATTAAAGATGTCCCTTCACCTGATGTGAGCAATCATCTAGTTTCAGAGGATGATCCTTCTGCTTTAAATGGAAACAGAGACCCGCTCACTTTTGATGGCATGGCAGATGCTGAGGTTGAAAGAAGACTGAAGGAAGCAACCTCAGCTGCTTTAACGGCTTCTTCGGTAGTCACTAACATAGATCCAAGGCTTGCTTCTCTTCAGTACTCTATGGCTCCTTCCTCTAGCACAACTTCACTGCCGTCAAGTCAGCAATCACCAATGACTTTTCCTAATATACAGTTCCCTCAGGGAGCTTCAGTGGTTAAACCGTTGGGTCATCTTGGCGCTGCAGAACCAAGCTTGCATAGTTCTCCGGCTAGAGAGGAGGGAGAGGTACCTGAATCAGAGTTAGACCCTGATACAAGGCGGAGGCTACTGATTTTGCAGCATGGCCAAGATACAAGAGAACCACCACCGAGTGAGCCCCCATTTGCTGTCAGACCTCCAGTACAAGCCTCTGTTCCACGGGTGCAACCGCGTCCAGGCTGGTTTCCAGTGGAGGAAGAAATGAGCCCTCGGCAACTGAGCCGGACGGTACCCAAAGAACTTCCATTGGATCCAGATCCAATGCAGATTGAGAAGCATCGACCTCATCATTCATCTTTTTTCTCAAAGGTTGATAATTCTATTCCATCTGATAGGATTCTTCAAGAAAATCAAAGATTTCCGAAAGAGGCATTTCATAGAGATGATCGGCTGAGATTCAACCATGCATCGGCTGGCTATCATTCAGTGTCAGGTGAGGAGATTCCCTTGAGTCGATCCCCTTCCATGAATAGAGATGTGGACTTTGAGTCTGGACGAGCTATATCAAATGCAGAAACTCCTGCTGGAGCCTTACAGGAAATCGCAATGAAGTGTGGAGCCAAGGTGGAGTTTAGGCCAGCTTTGGTTGCTAGCACGGAACTCCAGTTTTATGTTGAGGCTTGGTTTGCAGGAGAGAAAATTGGTGAAGGAACTGGTAAAACAAGAAGGGAAGCTCATTTCCAGGCTGCCGAGGGTTCTCTAAAAAACTTAGCCAATATATACCTTTCCCGCGTCAAGCCTGATTCTGTGCCTGTTCATGGGGAAATGAGCAAGTTTTCTAATGCTAACAACAATGGTTTTGTGGGTAATGCGAATTCTTTTGGGATCCAGTCATTTCCCAAAGAGGAGTCCCTGTCATCTTCAACTTCATCGGAGCCTTCTAGGCCACTAGACCCTAGGCTTGAAGGCTTCCAAAAATCTATGAATTCAGTCTCTGCCCTCAAAGAACTTTGTATGATAGAGGGCCTTGGTGGTGTAGTTTTTCAACCGCGACCTCCACCTTCAGCTAATTCAGTTGAGAAGGATGAAGTGCACGTACAGGTTGAAATAGATGGAGAGGTCTTGGGAAAGGGGATTGGGTTGACGTGGGATGAGGCTAAGATGCAGGCCGCTGAAAAGGCGCTCGGAAGTCTAAGATCAACTTTATTTGCTCAGAAACGTCAGGGTTCTCCAAGGTCTTTCCAAGGGATGCCAAATAAACGAATGAAGCAGGAGTTTCCACAAGTTCTTCAACGGATGCCCTCTTCTGCTAGATATCCGAAAAATGCTCCACCAGTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001101 GO:0003674 GO:0003824 GO:0004647 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008420 GO:0009611 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016311 GO:0016591 GO:0016787 GO:0016788 GO:0016791 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030880 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032870 GO:0032991 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070940 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0080090 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF dsrm

Protein Analysis

961

Amino Acids

107.33

Weight (kDa)

6.07

Isoelectric Point (pI)

59.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 253 - 357 3.8e-06 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 215
Acc36I ACCTGC 1 cut(s) 799
Acc65I GGTACC 2 cut(s) 1627, 1828
AccB1I GGYRCC 2 cut(s) 1627, 1828
AccBSI CCGCTC 2 cut(s) 241, 1336
AccII CGCG 3 cut(s) 1771, 2331, 2590
AccIII TCCGGA 1 cut(s) 229
AcoI YGGCCR 1 cut(s) 1684
AcsI RAATTY 4 cut(s) 1199, 1232, 2413, 2524
AcuI CTGAAG 5 cut(s) 1143, 1400, 1436, 1533, 2586
AcyI GRCGYC 1 cut(s) 212
AdeI CACNNNGTG 1 cut(s) 1712
AfaI GTAC 5 cut(s) 1457, 1629, 1743, 1830, 2637
AfiI CCNNNNNNNGG 7 cut(s) 299, 300, 301, 510, 1775, 2008, 2357
AhdI GACNNNNNGTC 1 cut(s) 2086
AjiI CACGTC 1 cut(s) 2685
AjnI CCWGG 3 cut(s) 332, 1774, 2280
AjuI GAANNNNNNNTTGG 4 cut(s) 18, 50, 1429, 1461
AleI CACNNNNGTG 1 cut(s) 527
Alw21I GWGCWC 1 cut(s) 2634
Alw26I GTCTC 4 cut(s) 231, 781, 1323, 2536
Alw44I GTGCAC 1 cut(s) 2630
AlwNI CAGNNNCTG 3 cut(s) 1142, 1361, 2534
Ama87I CYCGRG 1 cut(s) 1074
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 7 cut(s) 501, 701, 1684, 2174, 2483, 2563, 2706
ApaLI GTGCAC 1 cut(s) 2630
ApeKI GCWGC 4 cut(s) 1397, 1580, 1678, 2285
ApoI RAATTY 4 cut(s) 1199, 1232, 2413, 2524
AseI ATTAAT 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 804
Asp718I GGTACC 2 cut(s) 1627, 1828
AspA2I CCTAGG 2 cut(s) 295, 2495
AspLEI GCGC 4 cut(s) 480, 834, 1580, 2722
AspS9I GGNCC 3 cut(s) 502, 532, 2563
AsuHPI GGTGA 4 cut(s) 1260, 1500, 2045, 2255
AsuNHI GCTAGC 1 cut(s) 2189
AvaI CYCGRG 1 cut(s) 1074
AvaII GGWCC 1 cut(s) 532
AvrII CCTAGG 2 cut(s) 295, 2495
AxyI CCTNAGG 1 cut(s) 1539
BaeGI GKGCMC 1 cut(s) 2634
BaeI ACNNNNGTAYC 2 cut(s) 756, 789
BalI TGGCCA 1 cut(s) 1686
BamHI GGATCC 2 cut(s) 1849, 2424
BanI GGYRCC 2 cut(s) 1627, 1828
BanII GRGCYC 2 cut(s) 1719, 1808
BarI GAAGNNNNNNTAC 2 cut(s) 2619, 2651
BbsI GAAGAC 3 cut(s) 985, 1205, 1381
Bbv12I GWGCWC 1 cut(s) 2634
BbvCI CCTCAGC 2 cut(s) 1362, 1393
BbvI GCAGC 4 cut(s) 1384, 1567, 1690, 2272
BccI CCATC 6 cut(s) 193, 1015, 1020, 1340, 1933, 2645
BceAI ACGGC 3 cut(s) 202, 1422, 1477
BciT130I CCWGG 3 cut(s) 334, 1776, 2282
BclI TGATCA 1 cut(s) 616
BcoDI GTCTC 4 cut(s) 231, 781, 1323, 2536
BfaI CTAG 9 cut(s) 296, 1289, 1476, 1611, 2190, 2481, 2489, 2496, 2850
BfmI CTRYAG 3 cut(s) 282, 506, 1581
BfoI RGCGCY 2 cut(s) 1581, 2723
BfuAI ACCTGC 1 cut(s) 799
BglI GCCNNNNNGGC 1 cut(s) 2482
BglII AGATCT 1 cut(s) 802
BisI GCNGC 6 cut(s) 239, 1398, 1581, 1679, 2286, 2709
BlnI CCTAGG 2 cut(s) 295, 2495
BlsI GCNGC 6 cut(s) 240, 1399, 1582, 1680, 2287, 2710
BmcAI AGTACT 1 cut(s) 1457
Bme1390I CCNGG 3 cut(s) 334, 1776, 2282
Bme18I GGWCC 1 cut(s) 532
BmeRI GACNNNNNGTC 1 cut(s) 2086
BmeT110I CYCGRG 1 cut(s) 1074
BmgBI CACGTC 1 cut(s) 2685
BmgT120I GGNCC 3 cut(s) 502, 532, 2563
BmiI GGNNCC 9 cut(s) 534, 1467, 1629, 1830, 1851, 2128, 2158, 2426, 2475
BmrFI CCNGG 3 cut(s) 334, 1776, 2282
BmsI GCATC 7 cut(s) 389, 1348, 1885, 2015, 2691, 2778, 2826
BmtI GCTAGC 1 cut(s) 2193
BpiI GAAGAC 3 cut(s) 985, 1205, 1381
BplI GAGNNNNNCTC 2 cut(s) 1320, 1352
BpmI CTGGAG 3 cut(s) 1722, 2145, 2186
Bpu10I CCTNAGC 2 cut(s) 1362, 1393
BpuEI CTTGAG 2 cut(s) 1101, 2068
BsaAI YACGTR 2 cut(s) 542, 2635
BsaBI GATNNNNATC 1 cut(s) 1854
BsaHI GRCGYC 1 cut(s) 212
BsaI GGTCTC 2 cut(s) 781, 1323
BsaWI WCCGGW 1 cut(s) 229
BsaXI ACNNNNNCTCC 6 cut(s) 1536, 1566, 2148, 2178, 2438, 2468
Bsc4I CCNNNNNNNGG 7 cut(s) 299, 300, 301, 510, 1775, 2008, 2357
Bse1I ACTGG 7 cut(s) 146, 1739, 1787, 2203, 2257, 2428, 2873
Bse21I CCTNAGG 1 cut(s) 1539
Bse3DI GCAATG 2 cut(s) 1180, 2151
Bse8I GATNNNNATC 1 cut(s) 1854
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 3 cut(s) 334, 1776, 2282
BseGI GGATG 8 cut(s) 94, 1012, 1063, 1306, 2629, 2695, 2793, 2841
BseJI GATNNNNATC 1 cut(s) 1854
BseLI CCNNNNNNNGG 7 cut(s) 299, 300, 301, 510, 1775, 2008, 2357
BseMI GCAATG 2 cut(s) 1180, 2151
BseMII CTCAG 8 cut(s) 281, 516, 1353, 1407, 1553, 1808, 1982, 2765
BseNI ACTGG 7 cut(s) 146, 1739, 1787, 2203, 2257, 2428, 2873
BseRI GAGGAG 7 cut(s) 163, 247, 320, 1104, 1631, 2051, 2459
BseSI GKGCMC 1 cut(s) 2634
BseXI GCAGC 4 cut(s) 1384, 1567, 1690, 2272
Bsh1236I CGCG 3 cut(s) 1771, 2331, 2590
BshFI GGCC 7 cut(s) 503, 703, 1686, 2176, 2485, 2565, 2708
BshNI GGYRCC 2 cut(s) 1627, 1828
BsiHKAI GWGCWC 1 cut(s) 2634
BsiHKCI CYCGRG 1 cut(s) 1074
BsiSI CCGG 3 cut(s) 230, 1607, 1822
BslFI GGGAC 3 cut(s) 1124, 1247, 2434
BslI CCNNNNNNNGG 7 cut(s) 299, 300, 301, 510, 1775, 2008, 2357
BsmAI GTCTC 4 cut(s) 231, 781, 1323, 2536
BsmBI CGTCTC 1 cut(s) 231
BsmFI GGGAC 3 cut(s) 1124, 1247, 2434
BsnI GGCC 7 cut(s) 503, 703, 1686, 2176, 2485, 2565, 2708
Bso31I GGTCTC 2 cut(s) 781, 1323
BsoBI CYCGRG 1 cut(s) 1074
Bsp1286I GDGCHC 3 cut(s) 1719, 1808, 2634
Bsp13I TCCGGA 1 cut(s) 229
BspANI GGCC 7 cut(s) 503, 703, 1686, 2176, 2485, 2565, 2708
BspCNI CTCAG 8 cut(s) 280, 517, 1354, 1406, 1552, 1809, 1983, 2764
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 3 cut(s) 1771, 2331, 2590
BspLI GGNNCC 9 cut(s) 534, 1467, 1629, 1830, 1851, 2128, 2158, 2426, 2475
BspMAI CTGCAG 2 cut(s) 286, 1585
BspMI ACCTGC 1 cut(s) 799
BspOI GCTAGC 1 cut(s) 2193
BspT107I GGYRCC 2 cut(s) 1627, 1828
BspTNI GGTCTC 2 cut(s) 781, 1323
BsrBI CCGCTC 2 cut(s) 241, 1336
BsrDI GCAATG 2 cut(s) 1180, 2151
BsrI ACTGG 7 cut(s) 146, 1739, 1787, 2203, 2257, 2428, 2873
BssNI GRCGYC 1 cut(s) 212
BssT1I CCWWGG 7 cut(s) 295, 1436, 2160, 2495, 2566, 2771, 2782
Bst2UI CCWGG 3 cut(s) 334, 1776, 2282
Bst4CI ACNGT 7 cut(s) 61, 351, 1138, 1211, 1533, 1563, 1828
Bst6I CTCTTC 2 cut(s) 1455, 2848
BstACI GRCGYC 1 cut(s) 212
BstBAI YACGTR 2 cut(s) 542, 2635
BstC8I GCNNGC 9 cut(s) 558, 836, 1171, 1443, 1595, 2014, 2178, 2191, 2706
BstDSI CCRYGG 1 cut(s) 1757
BstF5I GGATG 8 cut(s) 94, 1012, 1063, 1306, 2629, 2695, 2793, 2841
BstFNI CGCG 3 cut(s) 1771, 2331, 2590
BstH2I RGCGCY 2 cut(s) 1581, 2723
BstHHI GCGC 4 cut(s) 480, 834, 1580, 2722
BstMAI GTCTC 4 cut(s) 231, 781, 1323, 2536
BstMWI GCNNNNNNNGC 7 cut(s) 1118, 1394, 1406, 2186, 2225, 2482, 2717
BstNI CCWGG 3 cut(s) 334, 1776, 2282
BstNSI RCATGY 1 cut(s) 1085
BstSCI CCNGG 3 cut(s) 332, 1774, 2280
BstSFI CTRYAG 3 cut(s) 282, 506, 1581
BstSLI GKGCMC 1 cut(s) 2634
BstSNI TACGTA 1 cut(s) 542
BstUI CGCG 3 cut(s) 1771, 2331, 2590
BstV1I GCAGC 4 cut(s) 1384, 1567, 1690, 2272
BstV2I GAAGAC 3 cut(s) 985, 1205, 1381
BstX2I RGATCY 5 cut(s) 802, 1432, 1849, 1855, 2424
BstXI CCANNNNNNTGG 1 cut(s) 363
BstYI RGATCY 5 cut(s) 802, 1432, 1849, 1855, 2424
Bsu36I CCTNAGG 1 cut(s) 1539
BsuRI GGCC 7 cut(s) 503, 703, 1686, 2176, 2485, 2565, 2708
BtgI CCRYGG 1 cut(s) 1757
BtrI CACGTC 1 cut(s) 2685
BtsCI GGATG 8 cut(s) 94, 1012, 1063, 1306, 2629, 2695, 2793, 2841
BtsI GCAGTG 2 cut(s) 159, 1487
BtsIMutI CAGTG 8 cut(s) 66, 153, 159, 522, 1487, 1557, 1794, 2031
BveI ACCTGC 1 cut(s) 799
Cac8I GCNNGC 9 cut(s) 558, 836, 1171, 1443, 1595, 2014, 2178, 2191, 2706
CaiI CAGNNNCTG 3 cut(s) 1142, 1361, 2534
CfoI GCGC 4 cut(s) 480, 834, 1580, 2722
Cfr13I GGNCC 3 cut(s) 502, 532, 2563
CseI GACGC 2 cut(s) 1760, 2320
Csp6I GTAC 5 cut(s) 1456, 1628, 1742, 1829, 2636
CviQI GTAC 5 cut(s) 1456, 1628, 1742, 1829, 2636
DraI TTTAAA 2 cut(s) 1195, 1317
DraIII CACNNNGTG 1 cut(s) 1712
DriI GACNNNNNGTC 1 cut(s) 2086
EaeI YGGCCR 1 cut(s) 1684
Eam1104I CTCTTC 2 cut(s) 1455, 2848
Eam1105I GACNNNNNGTC 1 cut(s) 2086
EarI CTCTTC 2 cut(s) 1455, 2848
EciI GGCGGA 1 cut(s) 1675
Eco105I TACGTA 1 cut(s) 542
Eco130I CCWWGG 7 cut(s) 295, 1436, 2160, 2495, 2566, 2771, 2782
Eco24I GRGCYC 2 cut(s) 1719, 1808
Eco31I GGTCTC 2 cut(s) 781, 1323
Eco32I GATATC 1 cut(s) 2855
Eco47I GGWCC 1 cut(s) 532
Eco57I CTGAAG 5 cut(s) 1143, 1400, 1436, 1533, 2586
Eco81I CCTNAGG 1 cut(s) 1539
Eco88I CYCGRG 1 cut(s) 1074
EcoO109I RGGNCCY 2 cut(s) 502, 2563
EcoRI GAATTC 2 cut(s) 2413, 2524
EcoRII CCWGG 3 cut(s) 332, 1774, 2280
EcoRV GATATC 1 cut(s) 2855
EcoT14I CCWWGG 7 cut(s) 295, 1436, 2160, 2495, 2566, 2771, 2782
EcoT22I ATGCAT 2 cut(s) 883, 2008
EcoT38I GRGCYC 2 cut(s) 1719, 1808
ErhI CCWWGG 7 cut(s) 295, 1436, 2160, 2495, 2566, 2771, 2782
Esp3I CGTCTC 1 cut(s) 231
FalI AAGNNNNNCTT 6 cut(s) 18, 50, 1429, 1461, 2725, 2757
FaqI GGGAC 3 cut(s) 1124, 1247, 2434
FauI CCCGC 2 cut(s) 1341, 2336
FbaI TGATCA 1 cut(s) 616
Fnu4HI GCNGC 6 cut(s) 239, 1398, 1581, 1679, 2286, 2709
FokI GGATG 8 cut(s) 101, 999, 1070, 1313, 2636, 2702, 2800, 2848
FriOI GRGCYC 2 cut(s) 1719, 1808
Fsp4HI GCNGC 6 cut(s) 239, 1398, 1581, 1679, 2286, 2709
FspBI CTAG 9 cut(s) 296, 1289, 1476, 1611, 2190, 2481, 2489, 2496, 2850
GlaI GCGC 4 cut(s) 479, 833, 1579, 2721
GluI GCNGC 6 cut(s) 239, 1398, 1581, 1679, 2286, 2709
GsuI CTGGAG 3 cut(s) 1722, 2145, 2186
HaeII RGCGCY 2 cut(s) 1581, 2723
HaeIII GGCC 7 cut(s) 503, 703, 1686, 2176, 2485, 2565, 2708
HapII CCGG 3 cut(s) 230, 1607, 1822
HgaI GACGC 2 cut(s) 1760, 2320
HhaI GCGC 4 cut(s) 480, 834, 1580, 2722
Hin1I GRCGYC 1 cut(s) 212
Hin6I GCGC 4 cut(s) 478, 832, 1578, 2720
HinP1I GCGC 4 cut(s) 478, 832, 1578, 2720
HincII GTYRAC 1 cut(s) 2682
HindII GTYRAC 1 cut(s) 2682
HindIII AAGCTT 2 cut(s) 1003, 1591
HpaII CCGG 3 cut(s) 230, 1607, 1822
HphI GGTGA 4 cut(s) 1260, 1500, 2045, 2255
Hpy166II GTNNAC 7 cut(s) 64, 143, 347, 532, 2080, 2632, 2682
Hpy8I GTNNAC 7 cut(s) 64, 143, 347, 532, 2080, 2632, 2682
Hpy99I CGWCG 1 cut(s) 217
HpyCH4III ACNGT 7 cut(s) 61, 351, 1138, 1211, 1533, 1563, 1828
HpyCH4IV ACGT 5 cut(s) 212, 541, 2634, 2684, 2758
HpyF10VI GCNNNNNNNGC 7 cut(s) 1118, 1394, 1406, 2186, 2225, 2482, 2717
HpySE526I ACGT 5 cut(s) 212, 541, 2634, 2684, 2758
Hsp92I GRCGYC 1 cut(s) 212
HspAI GCGC 4 cut(s) 478, 832, 1578, 2720
Kpn2I TCCGGA 1 cut(s) 229
KpnI GGTACC 2 cut(s) 1631, 1832
Ksp22I TGATCA 1 cut(s) 616
Lsp1109I GCAGC 4 cut(s) 1384, 1567, 1690, 2272
LweI GCATC 7 cut(s) 389, 1348, 1885, 2015, 2691, 2778, 2826
MaeI CTAG 9 cut(s) 296, 1289, 1476, 1611, 2190, 2481, 2489, 2496, 2850
MaeII ACGT 5 cut(s) 212, 541, 2634, 2684, 2758
MaeIII GTNAC 1 cut(s) 1420
MbiI CCGCTC 2 cut(s) 241, 1336
MflI RGATCY 5 cut(s) 802, 1432, 1849, 1855, 2424
MhlI GDGCHC 3 cut(s) 1719, 1808, 2634
MlsI TGGCCA 1 cut(s) 1686
MluNI TGGCCA 1 cut(s) 1686
MlyI GAGTC 6 cut(s) 212, 259, 733, 2059, 2096, 2456
MmeI TCCRAC 1 cut(s) 80
Mox20I TGGCCA 1 cut(s) 1686
Mph1103I ATGCAT 2 cut(s) 883, 2008
MroI TCCGGA 1 cut(s) 229
MroXI GAANNNNTTC 1 cut(s) 804
MscI TGGCCA 1 cut(s) 1686
MseI TTAA 6 cut(s) 747, 1194, 1254, 1316, 1403, 1557
MslI CAYNNNNRTG 2 cut(s) 527, 1980
Msp20I TGGCCA 1 cut(s) 1686
MspA1I CMGCKG 2 cut(s) 1397, 2711
MspI CCGG 3 cut(s) 230, 1607, 1822
MspR9I CCNGG 3 cut(s) 334, 1776, 2282
MvaI CCWGG 3 cut(s) 334, 1776, 2282
MvnI CGCG 3 cut(s) 1771, 2331, 2590
MwoI GCNNNNNNNGC 7 cut(s) 1118, 1394, 1406, 2186, 2225, 2482, 2717
NheI GCTAGC 1 cut(s) 2189
NlaIV GGNNCC 9 cut(s) 534, 1467, 1629, 1830, 1851, 2128, 2158, 2426, 2475
NmeAIII GCCGAG 2 cut(s) 1789, 2314
NmuCI GTSAC 1 cut(s) 1420
NsiI ATGCAT 2 cut(s) 883, 2008
NspI RCATGY 1 cut(s) 1085
OliI CACNNNNGTG 1 cut(s) 527
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PdmI GAANNNNTTC 1 cut(s) 804
PfoI TCCNGGA 1 cut(s) 332
PkrI GCNGC 6 cut(s) 240, 1399, 1582, 1680, 2287, 2710
PleI GAGTC 6 cut(s) 211, 259, 733, 2058, 2095, 2455
PpsI GAGTC 6 cut(s) 211, 259, 733, 2058, 2095, 2455
Ppu21I YACGTR 2 cut(s) 542, 2635
PshBI ATTAAT 1 cut(s) 747
Psp6I CCWGG 3 cut(s) 332, 1774, 2280
PspGI CCWGG 3 cut(s) 332, 1774, 2280
PspN4I GGNNCC 9 cut(s) 534, 1467, 1629, 1830, 1851, 2128, 2158, 2426, 2475
PspPI GGNCC 3 cut(s) 502, 532, 2563
PstI CTGCAG 2 cut(s) 286, 1585
PstNI CAGNNNCTG 3 cut(s) 1142, 1361, 2534
PsuI RGATCY 5 cut(s) 802, 1432, 1849, 1855, 2424
PvuII CAGCTG 1 cut(s) 1397
RsaI GTAC 5 cut(s) 1457, 1629, 1743, 1830, 2637
RsaNI GTAC 5 cut(s) 1456, 1628, 1742, 1829, 2636
RseI CAYNNNNRTG 2 cut(s) 527, 1980
SaqAI TTAA 6 cut(s) 747, 1194, 1254, 1316, 1403, 1557
SatI GCNGC 6 cut(s) 239, 1398, 1581, 1679, 2286, 2709
Sau96I GGNCC 3 cut(s) 502, 532, 2563
ScaI AGTACT 1 cut(s) 1457
SchI GAGTC 6 cut(s) 212, 259, 733, 2059, 2096, 2456
ScrFI CCNGG 3 cut(s) 334, 1776, 2282
SduI GDGCHC 3 cut(s) 1719, 1808, 2634
SfaNI GCATC 7 cut(s) 389, 1348, 1885, 2015, 2691, 2778, 2826
SfcI CTRYAG 3 cut(s) 282, 506, 1581
SinI GGWCC 1 cut(s) 532
SmiMI CAYNNNNRTG 2 cut(s) 527, 1980
SmlI CTYRAG 2 cut(s) 1116, 2047
SmoI CTYRAG 2 cut(s) 1116, 2047
SnaBI TACGTA 1 cut(s) 542
SspI AATATT 1 cut(s) 730
SspMI CTAG 9 cut(s) 296, 1289, 1476, 1611, 2190, 2481, 2489, 2496, 2850
StyD4I CCNGG 3 cut(s) 332, 1774, 2280
StyI CCWWGG 7 cut(s) 295, 1436, 2160, 2495, 2566, 2771, 2782
TaaI ACNGT 7 cut(s) 61, 351, 1138, 1211, 1533, 1563, 1828
TaiI ACGT 5 cut(s) 215, 544, 2637, 2687, 2761
TaqI TCGA 3 cut(s) 493, 1879, 2053
TatI WGTACW 2 cut(s) 1455, 1741
TauI GCSGC 2 cut(s) 241, 2711
Tru1I TTAA 6 cut(s) 747, 1194, 1254, 1316, 1403, 1557
Tru9I TTAA 6 cut(s) 747, 1194, 1254, 1316, 1403, 1557
TscAI CASTG 8 cut(s) 66, 153, 159, 529, 1494, 1557, 1794, 2031
TseFI GTSAC 1 cut(s) 1420
TseI GCWGC 4 cut(s) 1397, 1580, 1678, 2285
Tsp45I GTSAC 1 cut(s) 1420
TspGWI ACGGA 3 cut(s) 1092, 2210, 2848
TspRI CASTG 8 cut(s) 66, 153, 159, 529, 1494, 1557, 1794, 2031
VneI GTGCAC 1 cut(s) 2630
VpaK11BI GGWCC 1 cut(s) 532
VspI ATTAAT 1 cut(s) 747
XapI RAATTY 4 cut(s) 1199, 1232, 2413, 2524
XceI RCATGY 1 cut(s) 1085
XcmI CCANNNNNNNNNTGG 1 cut(s) 2009
XmaJI CCTAGG 2 cut(s) 295, 2495
XmnI GAANNNNTTC 1 cut(s) 804
XspI CTAG 9 cut(s) 296, 1289, 1476, 1611, 2190, 2481, 2489, 2496, 2850
ZraI GACGTC 1 cut(s) 213
ZrmI AGTACT 1 cut(s) 1457
Zsp2I ATGCAT 2 cut(s) 883, 2008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.