MD10G1297000.v1.1

RNA polymerase II C-terminal domain phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007472
Physical Location & Seq
Reverse (-)
38453471 .. 38462376
8906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1297000.v1.1.491

Sequence Viewer

Length: 2871 bp
ATGTATAAGTCGGTGTATAAAGGCGAAGATTTACTTGGGGAAGTAGAGATATATCCAACAGAGAACGAGAACAAGAATATTCAGGATGAGTTGAAGGAAATCAGAATAAGCTATTTTTCTCAACCAAGTGAAAGATGTCCACCAGTGGCAGTGCTTCATACCATAAGCTCCAATGGAGTTTGCTTCAAAATGATGGAGTCAAAGACGTCACCGTCGTCGTCTCAGGACACGCCGCTCTTTCTTTTGCACTCCTCCATGACTCAGGAGAACAAGACTGCAGTAATGCCGCTAGGAGGGGAGGAGCTTCATTTGGTTGCCATGCAATCCAGGAATGGTGATAAACAGTGTCCATGTTTTTGGGGATTCTATGTTGCATCAGGACTATACAATTCTTGCCTTGTCATGCTGAATCTTAGATGTCTGGGCATTGTATTTGATCTTGATGAAACACTCATTGTTGCAAATACAATGCGCTCCTTTGAAGATCGAATTGAGGCCTTGCAGAGGAAGATAAGCACTGAGGTGGACCCACAACGCATTTCTGGTATGCTTGCAGAGATCAAACGGTATCAAGATGACAAGTTTATATTGAAACAATATGCTGAAAATGATCAAGTTCTTGATAATGGGAGGGTCATCAAAACTCAAGCTGAGGTTGTTCCAGCCTTGTCTGACAATCATCAACCTATTATTCGGCCCCTTATACGATTACACGAGAAAAATATTATACTGACTCGCATTAATCCGCAGATTCGTGATACAAGTGTTCTCGTGAGGTTGAGACCTGCATGGGAGGATCTTCGGAGCTACCTGACTGCAAGGGGGCGCAAGCGGTTTGAGGTTTATGTTTGCACAATGGCTGAAAGGGATTATGCATTAGAAATGTGGAGGCTTCTTGATCCTGATTCAAATTTGATAAACCCAACTAAACTACTGGATCGCATTGTCTGTGTGAAGTCTGGTTCAAGAAAGTCGTTGTTCAGTGTCTTCCAAGAAAGCTTATGTCATCCTAAGATGGCATTGGTAATTGATGATCGCTTGAAAGTGTGGGATGACAGAGATCAACCTCGGGTGCATGTTGTTCCTGCATTTGCCCCGTATTATGCTCCTCAAGCTGAAGCAAATAATGCTGTCCCTGTCCTGTGTGTTGCAAGAAATGTTGCCTGCAATGTTAGAGGTGGATTTTTTAGAGAATTTGATGACAGTCTTCTACAAAAGATTCCTGAAATTTTTTATGAAGATGATATTAAAGATGTCCCTTCGCCTGATGTGAGCAATTATCTAGTTTCAGAGGATGACGGTTCTGCTATAAATGGAAACAGAGACCCACTTACTTTTGATGGCATGGCAGACATTGAAGTTGAAAGAAGACTGAAGGAAGCAACCTCAGCTGCTTTAACGGCTTCTTCAGTAGTCACTAATGTAGATCCAAGGCTTGCTTCTCTTCAGTACTCAATGGCTCCTTCCTCTAGCATAATTTCACTGCCATCAAGTCAGCCATCAGCGATGCATTTTCCTAGTATACAGTTCCCTCAGGCAGCTTCAGTAGTTAAACCGTTGGGTCATCTTGGCGCTGCAGAACCAAGCTTGCATAGTTCTCCTGCTAGAGAGGAGGGAGAGGTACCTGAATCAGAGTTAGACCCTGATACAAGACGGAGGCTACTGATTTTGCAGCATGGCCAAGATACAAGAGAACCACCACCAAGTGAGCCTCCATTTCCTGTCAGATCTCCAGTACAGGCCTCTGTTCCACGGGTGCAACCGCGTCCAGGCTGGTTTCCAGTGGAAGAAGAAATGAGCCCACGGCAACTGAGCCGGATGGTACCCAAAGAACTTCCGTTAGATCCAGATCCCATGCAGATTGAGAAGCATCGACCTCATCATTCATCTTTTTTCTCTAAGGTTGATAATTCTATTCCATCTGATAGGATTCTTCAAGAAAATCAAAGATTGCCAAAAGAGGCATTTCATAGAGATGATCGGCTGAGATTCAACCATGAATTGGCTGGCTATCATTCAATGTCAGGTGAGGAGATTCCCTTGAGTCGTTCCTCTTCCATGAATAGAGATGTGGACTTTGAATCTGGACAAGCTATATCAAATGCAGAAACTCCTGCTGGAGCCTTACAGGAAATCGCAATGAAGTGTGGAGCCAAGGTGGAGTTTAGGCCAGCTTTGGTTGCTAGCGCAGAACTGCAGTTTTATGTTGAGGCTTCGTTTGCAGGAGAGAAAATTGGTGAAGGAACTGGTAAAACAAGAAGGGAAGCTCATTTCCAGGCTGCTGAGGGTTCTCTTAAAAACTTAGCCAATGTTTACCTTTCCCGCTTCAAGCATGATTCTGTGCCTGTTCAAGGAGAAATGATTAAGTTTCCTAATGTTAACAACAATGGTTTTGTGGGTAATGCGAATTCTTTTGGGATCCAGTCATTTCCCAAAGATGAGTCCCTGTCATCTTCTTCAGAGTCTTCTAGGCCACTAGATCCTAGGCTTGAAGGCCCTAAAAAATCTATGAGTTCAGTATCTGCCCTCAAAGAACTTTGTATGATGGAGGGTCTTGGTGGTGTAGTTTTTCAACCACGACCTCCACCCTCTGCTAATTCAGTCGAGAAAGATGAAGTGCATGTACAGGTTGAAATAGATGGAGAGGTTCTGGGGAAGGGGATTGGGTTGACGTGGGATGAGGCTAAGATGCAGGCTGCTGAAAAGGCGCTCAGAAGTCTAAGACCAACTCTATTTGCTCAGAAACGTCAGGGTTCTCCAAGGTCTTTCCAAGGGATGCCAAATAAACGTATGAAGCAGGAGTTTCCACAAGTTCTGCAACGGATGCCCTCTTCTTCTAGATATCCGAAAAATGCTCCGCCAGTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001101 GO:0003674 GO:0003824 GO:0004647 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008420 GO:0009611 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016311 GO:0016591 GO:0016787 GO:0016788 GO:0016791 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030880 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032870 GO:0032991 GO:0033993 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070940 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0080090 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF dsrm

Protein Analysis

957

Amino Acids

107.03

Weight (kDa)

5.91

Isoelectric Point (pI)

59.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 251 - 355 5.5e-06 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 211
AatII GACGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 793
Acc65I GGTACC 2 cut(s) 1621, 1822
AccB1I GGYRCC 2 cut(s) 1621, 1822
AccB7I CCANNNNNTGG 1 cut(s) 2002
AccBSI CCGCTC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 1522
AccII CGCG 1 cut(s) 1765
AciI CCGC 7 cut(s) 233, 287, 746, 832, 1763, 2323, 2858
AclWI GGATC 9 cut(s) 804, 893, 945, 1421, 1838, 1844, 2413, 2426, 2474
AcoI YGGCCR 1 cut(s) 1678
AcsI RAATTY 4 cut(s) 910, 1193, 1227, 2407
AcuI CTGAAG 6 cut(s) 1137, 1392, 1394, 1430, 1527, 2442
AcyI GRCGYC 1 cut(s) 206
AdeI CACNNNGTG 1 cut(s) 1706
AfaI GTAC 5 cut(s) 1451, 1623, 1737, 1824, 2625
AfiI CCNNNNNNNGG 5 cut(s) 293, 504, 1769, 2002, 2351
AjiI CACGTC 1 cut(s) 2673
AjnI CCWGG 3 cut(s) 326, 1768, 2274
AjuI GAANNNNNNNTTGG 4 cut(s) 18, 50, 1423, 1455
AleI CACNNNNGTG 1 cut(s) 521
Alw26I GTCTC 3 cut(s) 225, 775, 1317
AlwI GGATC 9 cut(s) 804, 893, 945, 1421, 1838, 1844, 2413, 2426, 2474
AlwNI CAGNNNCTG 1 cut(s) 2522
Ama87I CYCGRG 1 cut(s) 1068
AoxI GGCC 7 cut(s) 495, 695, 1678, 1740, 2168, 2471, 2494
ApeKI GCWGC 6 cut(s) 1391, 1538, 1574, 1672, 2279, 2696
ApoI RAATTY 4 cut(s) 910, 1193, 1227, 2407
AseI ATTAAT 1 cut(s) 741
Asp718I GGTACC 2 cut(s) 1621, 1822
AspA2I CCTAGG 1 cut(s) 2483
AspLEI GCGC 5 cut(s) 474, 828, 1574, 2189, 2710
AspS9I GGNCC 3 cut(s) 526, 696, 2495
AsuHPI GGTGA 4 cut(s) 201, 347, 2039, 2249
AsuNHI GCTAGC 1 cut(s) 2183
AvaI CYCGRG 1 cut(s) 1068
AvaII GGWCC 1 cut(s) 526
AvrII CCTAGG 1 cut(s) 2483
AxyI CCTNAGG 1 cut(s) 1533
BaeI ACNNNNGTAYC 2 cut(s) 750, 783
BalI TGGCCA 1 cut(s) 1680
BamHI GGATCC 1 cut(s) 2418
BanI GGYRCC 2 cut(s) 1621, 1822
BanII GRGCYC 1 cut(s) 1802
BarI GAAGNNNNNNTAC 2 cut(s) 2607, 2639
BauI CACGAG 2 cut(s) 713, 770
BbsI GAAGAC 4 cut(s) 979, 1199, 1375, 2457
BbvCI CCTCAGC 3 cut(s) 651, 1387, 2283
BbvI GCAGC 6 cut(s) 1378, 1550, 1561, 1684, 2266, 2683
BccI CCATC 9 cut(s) 187, 1009, 1334, 1495, 1507, 1813, 1927, 2539, 2633
BceAI ACGGC 2 cut(s) 1416, 1820
BciT130I CCWGG 3 cut(s) 328, 1770, 2276
BclI TGATCA 1 cut(s) 610
BcoDI GTCTC 3 cut(s) 225, 775, 1317
BfmI CTRYAG 3 cut(s) 276, 1575, 2195
BfoI RGCGCY 2 cut(s) 1575, 2711
BfuAI ACCTGC 1 cut(s) 793
BglII AGATCT 1 cut(s) 1727
BisI GCNGC 8 cut(s) 233, 287, 1392, 1539, 1575, 1673, 2280, 2697
BlnI CCTAGG 1 cut(s) 2483
BlsI GCNGC 8 cut(s) 234, 288, 1393, 1540, 1576, 1674, 2281, 2698
BmcAI AGTACT 1 cut(s) 1451
Bme1390I CCNGG 3 cut(s) 328, 1770, 2276
Bme18I GGWCC 1 cut(s) 526
BmeT110I CYCGRG 1 cut(s) 1068
BmgBI CACGTC 1 cut(s) 2673
BmgT120I GGNCC 3 cut(s) 526, 696, 2495
BmiI GGNNCC 8 cut(s) 528, 698, 1461, 1623, 1824, 2122, 2152, 2420
BmrFI CCNGG 3 cut(s) 328, 1770, 2276
BmsI GCATC 6 cut(s) 383, 1497, 1879, 2679, 2766, 2814
BmtI GCTAGC 1 cut(s) 2187
BpiI GAAGAC 4 cut(s) 979, 1199, 1375, 2457
BpmI CTGGAG 2 cut(s) 1716, 2139
Bpu10I CCTNAGC 3 cut(s) 651, 1387, 2283
BpuEI CTTGAG 3 cut(s) 630, 1095, 2062
BsaBI GATNNNNATC 1 cut(s) 1848
BsaHI GRCGYC 1 cut(s) 206
BsaI GGTCTC 2 cut(s) 775, 1317
BsaJI CCNNGG 8 cut(s) 1067, 1430, 1751, 1802, 2154, 2483, 2759, 2770
BsaXI ACNNNNNCTCC 2 cut(s) 2142, 2172
Bsc4I CCNNNNNNNGG 5 cut(s) 293, 504, 1769, 2002, 2351
Bse1I ACTGG 7 cut(s) 143, 939, 1733, 1781, 2251, 2422, 2861
Bse21I CCTNAGG 1 cut(s) 1533
Bse3DI GCAATG 2 cut(s) 1174, 2145
Bse8I GATNNNNATC 1 cut(s) 1848
BseBI CCWGG 3 cut(s) 328, 1770, 2276
BseDI CCNNGG 8 cut(s) 1067, 1430, 1751, 1802, 2154, 2483, 2759, 2770
BseGI GGATG 8 cut(s) 91, 1006, 1057, 1300, 1824, 2683, 2781, 2829
BseJI GATNNNNATC 1 cut(s) 1848
BseLI CCNNNNNNNGG 5 cut(s) 293, 504, 1769, 2002, 2351
BseMI GCAATG 2 cut(s) 1174, 2145
BseNI ACTGG 7 cut(s) 143, 939, 1733, 1781, 2251, 2422, 2861
BseRI GAGGAG 5 cut(s) 241, 314, 1098, 1625, 2045
BseXI GCAGC 6 cut(s) 1378, 1550, 1561, 1684, 2266, 2683
Bsh1236I CGCG 1 cut(s) 1765
BshFI GGCC 7 cut(s) 497, 697, 1680, 1742, 2170, 2473, 2496
BshNI GGYRCC 2 cut(s) 1621, 1822
BsiHKCI CYCGRG 1 cut(s) 1068
BsiSI CCGG 1 cut(s) 1816
BslFI GGGAC 3 cut(s) 1118, 1241, 2428
BslI CCNNNNNNNGG 5 cut(s) 293, 504, 1769, 2002, 2351
BsmAI GTCTC 3 cut(s) 225, 775, 1317
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 3 cut(s) 1118, 1241, 2428
BsnI GGCC 7 cut(s) 497, 697, 1680, 1742, 2170, 2473, 2496
Bso31I GGTCTC 2 cut(s) 775, 1317
BsoBI CYCGRG 1 cut(s) 1068
Bsp1286I GDGCHC 1 cut(s) 1802
Bsp1407I TGTACA 1 cut(s) 2623
BspACI CCGC 7 cut(s) 233, 287, 746, 832, 1763, 2323, 2858
BspANI GGCC 7 cut(s) 497, 697, 1680, 1742, 2170, 2473, 2496
BspFNI CGCG 1 cut(s) 1765
BspLI GGNNCC 8 cut(s) 528, 698, 1461, 1623, 1824, 2122, 2152, 2420
BspMAI CTGCAG 3 cut(s) 280, 1579, 2199
BspMI ACCTGC 1 cut(s) 793
BspOI GCTAGC 1 cut(s) 2187
BspPI GGATC 9 cut(s) 804, 893, 945, 1421, 1838, 1844, 2413, 2426, 2474
BspT107I GGYRCC 2 cut(s) 1621, 1822
BspTNI GGTCTC 2 cut(s) 775, 1317
BsrBI CCGCTC 1 cut(s) 235
BsrDI GCAATG 2 cut(s) 1174, 2145
BsrGI TGTACA 1 cut(s) 2623
BsrI ACTGG 7 cut(s) 143, 939, 1733, 1781, 2251, 2422, 2861
BssECI CCNNGG 8 cut(s) 1067, 1430, 1751, 1802, 2154, 2483, 2759, 2770
BssNAI GTATAC 1 cut(s) 1523
BssNI GRCGYC 1 cut(s) 206
BssSI CACGAG 2 cut(s) 713, 770
BssT1I CCWWGG 5 cut(s) 1430, 2154, 2483, 2759, 2770
Bst1107I GTATAC 1 cut(s) 1523
Bst2BI CACGAG 2 cut(s) 713, 770
Bst2UI CCWGG 3 cut(s) 328, 1770, 2276
Bst4CI ACNGT 7 cut(s) 213, 345, 567, 1205, 1301, 1527, 1557
Bst6I CTCTTC 3 cut(s) 1449, 2059, 2836
BstACI GRCGYC 1 cut(s) 206
BstAPI GCANNNNNTGC 2 cut(s) 1127, 2824
BstAUI TGTACA 1 cut(s) 2623
BstC8I GCNNGC 9 cut(s) 552, 830, 1165, 1437, 1589, 2008, 2172, 2185, 2694
BstDSI CCRYGG 2 cut(s) 1751, 1802
BstENI CCTNNNNNAGG 2 cut(s) 502, 2349
BstF5I GGATG 8 cut(s) 91, 1006, 1057, 1300, 1824, 2683, 2781, 2829
BstFNI CGCG 1 cut(s) 1765
BstH2I RGCGCY 2 cut(s) 1575, 2711
BstHHI GCGC 5 cut(s) 474, 828, 1574, 2189, 2710
BstMAI GTCTC 3 cut(s) 225, 775, 1317
BstMWI GCNNNNNNNGC 8 cut(s) 1112, 1127, 1388, 1400, 2180, 2219, 2705, 2824
BstNI CCWGG 3 cut(s) 328, 1770, 2276
BstNSI RCATGY 2 cut(s) 1079, 2624
BstSCI CCNGG 3 cut(s) 326, 1768, 2274
BstSFI CTRYAG 3 cut(s) 276, 1575, 2195
BstUI CGCG 1 cut(s) 1765
BstV1I GCAGC 6 cut(s) 1378, 1550, 1561, 1684, 2266, 2683
BstV2I GAAGAC 4 cut(s) 979, 1199, 1375, 2457
BstX2I RGATCY 7 cut(s) 796, 1426, 1727, 1843, 1849, 2418, 2479
BstXI CCANNNNNNTGG 1 cut(s) 357
BstYI RGATCY 7 cut(s) 796, 1426, 1727, 1843, 1849, 2418, 2479
BstZ17I GTATAC 1 cut(s) 1523
Bsu36I CCTNAGG 1 cut(s) 1533
BsuRI GGCC 7 cut(s) 497, 697, 1680, 1742, 2170, 2473, 2496
BtgI CCRYGG 2 cut(s) 1751, 1802
BtgZI GCGATG 1 cut(s) 1520
BtrI CACGTC 1 cut(s) 2673
BtsCI GGATG 8 cut(s) 91, 1006, 1057, 1300, 1824, 2683, 2781, 2829
BtsI GCAGTG 2 cut(s) 156, 1481
BtsIMutI CAGTG 7 cut(s) 150, 156, 350, 516, 988, 1481, 1788
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 9 cut(s) 552, 830, 1165, 1437, 1589, 2008, 2172, 2185, 2694
CaiI CAGNNNCTG 1 cut(s) 2522
CfoI GCGC 5 cut(s) 474, 828, 1574, 2189, 2710
Cfr13I GGNCC 3 cut(s) 526, 696, 2495
CseI GACGC 1 cut(s) 1754
Csp6I GTAC 5 cut(s) 1450, 1622, 1736, 1823, 2624
CviQI GTAC 5 cut(s) 1450, 1622, 1736, 1823, 2624
DraIII CACNNNGTG 1 cut(s) 1706
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 1678
Eam1104I CTCTTC 3 cut(s) 1449, 2059, 2836
EarI CTCTTC 3 cut(s) 1449, 2059, 2836
EciI GGCGGA 1 cut(s) 2847
Eco130I CCWWGG 5 cut(s) 1430, 2154, 2483, 2759, 2770
Eco147I AGGCCT 2 cut(s) 497, 1742
Eco24I GRGCYC 1 cut(s) 1802
Eco31I GGTCTC 2 cut(s) 775, 1317
Eco32I GATATC 1 cut(s) 2843
Eco47I GGWCC 1 cut(s) 526
Eco57I CTGAAG 6 cut(s) 1137, 1392, 1394, 1430, 1527, 2442
Eco81I CCTNAGG 1 cut(s) 1533
Eco88I CYCGRG 1 cut(s) 1068
EcoNI CCTNNNNNAGG 2 cut(s) 502, 2349
EcoO109I RGGNCCY 1 cut(s) 2495
EcoRI GAATTC 1 cut(s) 2407
EcoRII CCWGG 3 cut(s) 326, 1768, 2274
EcoRV GATATC 1 cut(s) 2843
EcoT14I CCWWGG 5 cut(s) 1430, 2154, 2483, 2759, 2770
EcoT22I ATGCAT 2 cut(s) 877, 1512
EcoT38I GRGCYC 1 cut(s) 1802
ErhI CCWWGG 5 cut(s) 1430, 2154, 2483, 2759, 2770
Esp3I CGTCTC 1 cut(s) 225
FalI AAGNNNNNCTT 4 cut(s) 18, 50, 1423, 1455
FaqI GGGAC 3 cut(s) 1118, 1241, 2428
FauI CCCGC 1 cut(s) 2330
FbaI TGATCA 1 cut(s) 610
FblI GTMKAC 1 cut(s) 1522
Fnu4HI GCNGC 8 cut(s) 233, 287, 1392, 1539, 1575, 1673, 2280, 2697
FokI GGATG 8 cut(s) 98, 993, 1064, 1307, 1831, 2690, 2788, 2836
FriOI GRGCYC 1 cut(s) 1802
Fsp4HI GCNGC 8 cut(s) 233, 287, 1392, 1539, 1575, 1673, 2280, 2697
GlaI GCGC 5 cut(s) 473, 827, 1573, 2188, 2709
GluI GCNGC 8 cut(s) 233, 287, 1392, 1539, 1575, 1673, 2280, 2697
GsuI CTGGAG 2 cut(s) 1716, 2139
HaeII RGCGCY 2 cut(s) 1575, 2711
HaeIII GGCC 7 cut(s) 497, 697, 1680, 1742, 2170, 2473, 2496
HapII CCGG 1 cut(s) 1816
HgaI GACGC 1 cut(s) 1754
HhaI GCGC 5 cut(s) 474, 828, 1574, 2189, 2710
Hin1I GRCGYC 1 cut(s) 206
Hin6I GCGC 5 cut(s) 472, 826, 1572, 2187, 2708
HinP1I GCGC 5 cut(s) 472, 826, 1572, 2187, 2708
HincII GTYRAC 2 cut(s) 2380, 2670
HindII GTYRAC 2 cut(s) 2380, 2670
HindIII AAGCTT 2 cut(s) 997, 1585
HpaI GTTAAC 1 cut(s) 2380
HpaII CCGG 1 cut(s) 1816
HphI GGTGA 4 cut(s) 201, 347, 2039, 2249
Hpy166II GTNNAC 7 cut(s) 140, 526, 1523, 2074, 2314, 2380, 2670
Hpy8I GTNNAC 7 cut(s) 140, 526, 1523, 2074, 2314, 2380, 2670
Hpy99I CGWCG 2 cut(s) 217, 220
HpyAV CCTTC 8 cut(s) 88, 1269, 1369, 1473, 2234, 2253, 2486, 2650
HpyCH4III ACNGT 7 cut(s) 213, 345, 567, 1205, 1301, 1527, 1557
HpyCH4IV ACGT 4 cut(s) 206, 2672, 2746, 2788
HpyF10VI GCNNNNNNNGC 8 cut(s) 1112, 1127, 1388, 1400, 2180, 2219, 2705, 2824
HpySE526I ACGT 4 cut(s) 206, 2672, 2746, 2788
Hsp92I GRCGYC 1 cut(s) 206
HspAI GCGC 5 cut(s) 472, 826, 1572, 2187, 2708
KpnI GGTACC 2 cut(s) 1625, 1826
Ksp22I TGATCA 1 cut(s) 610
KspAI GTTAAC 1 cut(s) 2380
LmnI GCTCC 9 cut(s) 173, 301, 479, 804, 1111, 1465, 2120, 2150, 2860
Lsp1109I GCAGC 6 cut(s) 1378, 1550, 1561, 1684, 2266, 2683
LweI GCATC 6 cut(s) 383, 1497, 1879, 2679, 2766, 2814
MaeII ACGT 4 cut(s) 206, 2672, 2746, 2788
MaeIII GTNAC 2 cut(s) 207, 1414
MbiI CCGCTC 1 cut(s) 235
MflI RGATCY 7 cut(s) 796, 1426, 1727, 1843, 1849, 2418, 2479
MhlI GDGCHC 1 cut(s) 1802
MlsI TGGCCA 1 cut(s) 1680
MluNI TGGCCA 1 cut(s) 1680
MlyI GAGTC 6 cut(s) 206, 253, 727, 2053, 2450, 2471
MmeI TCCRAC 1 cut(s) 80
Mox20I TGGCCA 1 cut(s) 1680
Mph1103I ATGCAT 2 cut(s) 877, 1512
MscI TGGCCA 1 cut(s) 1680
MseI TTAA 7 cut(s) 741, 1248, 1397, 1551, 2295, 2364, 2379
MslI CAYNNNNRTG 2 cut(s) 521, 1974
Msp20I TGGCCA 1 cut(s) 1680
MspA1I CMGCKG 1 cut(s) 1391
MspI CCGG 1 cut(s) 1816
MspR9I CCNGG 3 cut(s) 328, 1770, 2276
MvaI CCWGG 3 cut(s) 328, 1770, 2276
MvnI CGCG 1 cut(s) 1765
MwoI GCNNNNNNNGC 8 cut(s) 1112, 1127, 1388, 1400, 2180, 2219, 2705, 2824
NheI GCTAGC 1 cut(s) 2183
NlaIV GGNNCC 8 cut(s) 528, 698, 1461, 1623, 1824, 2122, 2152, 2420
NmuCI GTSAC 2 cut(s) 207, 1414
NsiI ATGCAT 2 cut(s) 877, 1512
NspI RCATGY 2 cut(s) 1079, 2624
OliI CACNNNNGTG 1 cut(s) 521
PceI AGGCCT 2 cut(s) 497, 1742
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PflMI CCANNNNNTGG 1 cut(s) 2002
PfoI TCCNGGA 1 cut(s) 326
PkrI GCNGC 8 cut(s) 234, 288, 1393, 1540, 1576, 1674, 2281, 2698
PleI GAGTC 6 cut(s) 205, 253, 727, 2052, 2449, 2470
PpsI GAGTC 6 cut(s) 205, 253, 727, 2052, 2449, 2470
PshBI ATTAAT 1 cut(s) 741
Psp6I CCWGG 3 cut(s) 326, 1768, 2274
PspGI CCWGG 3 cut(s) 326, 1768, 2274
PspN4I GGNNCC 8 cut(s) 528, 698, 1461, 1623, 1824, 2122, 2152, 2420
PspPI GGNCC 3 cut(s) 526, 696, 2495
PstI CTGCAG 3 cut(s) 280, 1579, 2199
PstNI CAGNNNCTG 1 cut(s) 2522
PsuI RGATCY 7 cut(s) 796, 1426, 1727, 1843, 1849, 2418, 2479
PvuII CAGCTG 1 cut(s) 1391
RsaI GTAC 5 cut(s) 1451, 1623, 1737, 1824, 2625
RsaNI GTAC 5 cut(s) 1450, 1622, 1736, 1823, 2624
RseI CAYNNNNRTG 2 cut(s) 521, 1974
SaqAI TTAA 7 cut(s) 741, 1248, 1397, 1551, 2295, 2364, 2379
SatI GCNGC 8 cut(s) 233, 287, 1392, 1539, 1575, 1673, 2280, 2697
Sau96I GGNCC 3 cut(s) 526, 696, 2495
ScaI AGTACT 1 cut(s) 1451
SchI GAGTC 6 cut(s) 206, 253, 727, 2053, 2450, 2471
ScrFI CCNGG 3 cut(s) 328, 1770, 2276
SduI GDGCHC 1 cut(s) 1802
SfaNI GCATC 6 cut(s) 383, 1497, 1879, 2679, 2766, 2814
SfcI CTRYAG 3 cut(s) 276, 1575, 2195
SinI GGWCC 1 cut(s) 526
SmiMI CAYNNNNRTG 2 cut(s) 521, 1974
SmlI CTYRAG 3 cut(s) 645, 1110, 2041
SmoI CTYRAG 3 cut(s) 645, 1110, 2041
SseBI AGGCCT 2 cut(s) 497, 1742
SsiI CCGC 7 cut(s) 233, 287, 746, 832, 1763, 2323, 2858
SspI AATATT 2 cut(s) 79, 724
StuI AGGCCT 2 cut(s) 497, 1742
StyD4I CCNGG 3 cut(s) 326, 1768, 2274
StyI CCWWGG 5 cut(s) 1430, 2154, 2483, 2759, 2770
TaaI ACNGT 7 cut(s) 213, 345, 567, 1205, 1301, 1527, 1557
TaiI ACGT 4 cut(s) 209, 2675, 2749, 2791
TaqI TCGA 3 cut(s) 487, 1873, 2604
TatI WGTACW 3 cut(s) 1449, 1735, 2623
TauI GCSGC 2 cut(s) 235, 289
Tru1I TTAA 7 cut(s) 741, 1248, 1397, 1551, 2295, 2364, 2379
Tru9I TTAA 7 cut(s) 741, 1248, 1397, 1551, 2295, 2364, 2379
TscAI CASTG 7 cut(s) 150, 156, 350, 523, 988, 1488, 1788
TseFI GTSAC 2 cut(s) 207, 1414
TseI GCWGC 6 cut(s) 1391, 1538, 1574, 1672, 2279, 2696
Tsp45I GTSAC 2 cut(s) 207, 1414
TspGWI ACGGA 3 cut(s) 1669, 1827, 2836
TspRI CASTG 7 cut(s) 150, 156, 350, 523, 988, 1488, 1788
Van91I CCANNNNNTGG 1 cut(s) 2002
VpaK11BI GGWCC 1 cut(s) 526
VspI ATTAAT 1 cut(s) 741
XagI CCTNNNNNAGG 2 cut(s) 502, 2349
XapI RAATTY 4 cut(s) 910, 1193, 1227, 2407
XbaI TCTAGA 1 cut(s) 2837
XceI RCATGY 2 cut(s) 1079, 2624
XcmI CCANNNNNNNNNTGG 1 cut(s) 2003
XmaJI CCTAGG 1 cut(s) 2483
XmiI GTMKAC 1 cut(s) 1522
ZraI GACGTC 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 1451
Zsp2I ATGCAT 2 cut(s) 877, 1512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.