Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005133

Physical Location & Seq

Genomic Coordinates Reverse (-)

45124064 .. 45131620

Gene Structure 7557 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1324700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2691 bp

ATGTCGTCGTCTTCGTCGTCTTCCAAGGTCTGCTACAATTCTGAGTGCAAGGAGGTGAAATCGGAGTTTCCGAGAAAGGGCTGGCGGCTCCGGACTGGCGAGTACTGCGAGCTCTGCGATCGGTGCTATGTTGCTTATGAGGAAGGGAGATTCTGTGAGACCTTTCATACGAATGCTGCTGGTTGGAGGTGCTGTGAGTCTTGTGGGAAGCGGGTTCATTGTGGATGCATCGTTTCAAGTCACGCATTTACATTGTTGGATCCCGGAGGAATTGAGTGCATGACATGTGCAAGGAAAAATTATGTAATGACATCAAACCCAGCATGGCCAACATCTATGTTCTTCCATTCACCTTTATCTGAGAGACTTAAGGACCTGTCTACCAAAAATTGGACTCAGTTGGCTGGATCTGGTCCTGTACCATGGAGGCAAGCACCCAGTTTATTCAATTCTTCTATTCCTTCATCTGAGTTGCATACGAGGGTGCCACATGAAGTTGATGGTTCAACTGGAATTAACAAGCTTAATGCCAGTGAGAGAATATTTGTCTCCACCTTGGAAAAGAAGAAAGTTGAGGAATTTTCTGAAAGACTAATGAAAGTAAACCTAAGTATTGGCACACAACAGAGTCTTGAAAATGGGATTGGAAACCCAGGAATTAACCACGAGGAGAAAAGTAGTTCATGTTTAAATATATCCCAGCAGTCTGCCTCTTTGAAGGAGGAACCGTCTACTCCACAGTTTGTACCTTATGGATCTCCAAGTGAAACAAATCCACAACTGGGGGTTACTGGAACTAATTTGCGGCCAATCGCTCCTTTGGCAAAGAATTATAATGGTAATGGGGTTGAATCATCTGGTGATAATCAGATCCGCAATGCAAGGCCTCGAGCAGATGCCCGGGGAAGAAATCAGTTACTTCCTCGGTACTGGCCTAGATTCACTGATCAAGAGCTGCAGCATATATCTGGAGATAAAAATTCTGTAATCACTCCATTATTCGAGAAAATGTTGAGCGCTAGTGATGCTGGGCGGATCGGGCGTTTGGTGTTGCCAAAAAAATGTGCAGAGGCCTACTTTCCTCCAATTTCTCAGCCCGAAGGGTTGCCTCTAAAAGTCCAGGATTCAAAAGGCAAGGAATGGATATTTCAGTTTCGCTTCTGGCCCAACAACAATAGCAGAATGTATGTTTTGGAGGGGGTTACCCCTTGCATACAATCCATGCAATTGCTGGCCGGTGACATAGTGACATTTAGTCGATTGGAGCCAGAAGGGAAATTGGTTATGGGGTTCAGAAAGGCTTCAACTTCCCTACAATCTGATCAGGAAAATGAGGCAAACAAGACAACCAACGGAGTTTCGGTCCATGCAGAAGTTGAATTGGCAGATCCTAGCTCATGGTCTAATGTTGATAAGTCAGGTTACATAGCAAAAGAAGCACTGGAAGATAAATCTTTGATCCCTAAAAAAAGGAAGAACAGTAACTTGGGTTCTAAGAGTAAGCGTCTGAGAATTGAAAATGAAGACCTGATAGAACTGAAGGTTACATGGGAAGAAGCTCAAGGCCTGCTTCGCCCTCCTGCTAACCATGTTCCCACTGTTGTGGTGATTGAAGGCTTTGAGTTTGAGGAATATGAGGATGCTCCAATTCTTGGGAAGCCAACAATTTATGCGACGGGCAGAGCAGGTGAGAAGATTCAGTGGGTTCAATGCGAAGATTGTTTCAAATGGCGCAAACTGCCAATCAATGCTCTTCTACCATCAAAGTGGACTTGTTCTGAGAATTCATGGGACCCTGAAAGATCTTTGTGTTCAGCAGCTCAAGAATTGACAACGGAACAGCTCCAAGATTTGCTACCCCGCTGTAATACAGTTGCTTCGAAGAAAATGAAGGCTGCTAAACAGGACCCCGATAACATTGAAGTTCTGGAGGGCCTTGACACACTTGCGAACCTAGCCATCTTGGGAGAGGGTGAGTCGCTCCCATCATCAGCTCAGGCCACCACAAAGCACCCCCGACATAGGCCCGGATGCTCATGTATTGTCTGCATCCAGCCCCCTAGTGGGAAGGGTCCTAAACACAAGCAAACATGCACCTGCAATGTATGCTTAACAGTGAAGCGTCGCTTCCGTACATTAATGATGCGGCGTGAGAAGAAACAGTCAGATAAAGAAGCAGAAACTACACGCAGAAAGCAGCACAAGGGGCAATTACTGGTGGTTGATAAACAGGAGGACGACACCCTATCATATTGTAACGTAGGAAGCAGCAGTCCAAACCAGAAAATAGAGATCCTTGAACTTTCCGATGATGACCTTAACATGACGAAATCCTCAACTTCACCTTTCAAAGGCCAAATTGACCTGAATATCCAGCCAGAACGCGAGGATGAGCTGTCACCTGGATCCGATTCAGGCGGCATGATGAAGTTGCTTCACGATGCCACAGCAAGGTATCTCAGGCAGCAGAGATTGTCAAGCTCTGGTGGTAGTGAAAAGTCATCAGGCAACGGGTCACAACGAGCTGCAAGTGAAGCAGGTGGAGGGGGAGGGGGAGAACCCCTCAGCAATGGTGTAAACCTCGAAAGTAGCAATGGTGTAAACCTCGAAAATAGCAATCATGACAGCAGCAACGACTGTCCTGCAACTTTATCCATCAAACCATCGGCATCCATGACTGCCACGGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B3 zf-CW

Protein Analysis

897

Amino Acids

98.8

Weight (kDa)

7.42

Isoelectric Point (pI)

50.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf_VAL1_N	PF25813	54 - 101	1.2e-22	VAL1-like, N-terminal zinc finger domain
B3	PF02362	334 - 433	1.7e-15	B3 DNA binding domain
zf-CW	PF07496	567 - 609	6.7e-12	CW-type Zinc Finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21550 AT4G21550 AT4G21550
fragaria_vesca	FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120
malus_domestica	MD05G1324700.v1.1 MD10G1302500.v1.1
prunus_persica	Prupe.4G036100_v2.0.a1
pyrus_communis	pycom05g29890 pycom10g25500
rosa_chinensis	RchiOBHm_Chr5g0006571
rosa_laevigata	RLG00000031400
rosa_multiflora	Rmu_sc0007807.1_g000001 Rmu_sc0011154.1_g000008 Rmu_sc0011154.1_g000009
rosa_roxburghii	Rroxscaffold_1G00069470
rosa_rugosa	Rorug04G0422600.1 Rorug04G0422700
rosa_samantha	Rh5AG049800 Rh5BG049100 Rh5CG057400 Rh5DG048500
rosa_wichuraiana	Rw5G004990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	834
AarI	CACCTGC	3 cut(s)	1676, 2105, 2528
Acc36I	ACCTGC	3 cut(s)	1676, 2105, 2528
AccB1I	GGYRCC	1 cut(s)	484
AccB7I	CCANNNNNTGG	2 cut(s)	390, 1652
AccI	GTMKAC	2 cut(s)	380, 731
AccII	CGCG	1 cut(s)	2385
AccIII	TCCGGA	1 cut(s)	90
AciI	CCGC	8 cut(s)	85, 211, 805, 874, 1033, 1861, 2146, 2418
AcoI	YGGCCR	3 cut(s)	326, 806, 1233
AcsI	RAATTY	3 cut(s)	578, 979, 1783
AcuI	CTGAAG	1 cut(s)	1559
AfaI	GTAC	5 cut(s)	104, 420, 747, 929, 2134
AfeI	AGCGCT	1 cut(s)	1018
AfiI	CCNNNNNNNGG	9 cut(s)	77, 390, 782, 1652, 2022, 2063, 2064, 2351, 2451
AflII	CTTAAG	1 cut(s)	368
AflIII	ACRYGT	1 cut(s)	284
AhdI	GACNNNNNGTC	1 cut(s)	1254
AjnI	CCWGG	3 cut(s)	652, 1119, 2401
AjuI	GAANNNNNNNTTGG	4 cut(s)	627, 659, 1469, 1501
AleI	CACNNNNGTG	1 cut(s)	1601
Alw21I	GWGCWC	1 cut(s)	114
Alw26I	GTCTC	3 cut(s)	152, 358, 553
Ama87I	CYCGRG	2 cut(s)	888, 900
Aor13HI	TCCGGA	1 cut(s)	90
Aor51HI	AGCGCT	1 cut(s)	1018
ApoI	RAATTY	3 cut(s)	578, 979, 1783
AseI	ATTAAT	1 cut(s)	2138
Asp700I	GAANNNNTTC	1 cut(s)	1300
AspLEI	GCGC	2 cut(s)	1019, 1734
AspS9I	GGNCC	9 cut(s)	373, 413, 1164, 1363, 1792, 1906, 1933, 2025, 2072
AsuC2I	CCSGG	4 cut(s)	264, 901, 902, 2028
AsuHPI	GGTGA	9 cut(s)	67, 342, 872, 1250, 1618, 1700, 1985, 2334, 2391
AsuII	TTCGAA	1 cut(s)	1880
AvaI	CYCGRG	2 cut(s)	888, 900
AvaII	GGWCC	6 cut(s)	373, 413, 1363, 1792, 1906, 2072
BalI	TGGCCA	1 cut(s)	328
BamHI	GGATCC	2 cut(s)	259, 2405
BanI	GGYRCC	1 cut(s)	484
BanII	GRGCYC	1 cut(s)	114
BauI	CACGAG	1 cut(s)	665
BbsI	GAAGAC	3 cut(s)	3, 12, 1530
Bbv12I	GWGCWC	1 cut(s)	114
BbvCI	CCTCAGC	1 cut(s)	2564
BccI	CCATC	6 cut(s)	494, 1768, 1967, 1993, 2663, 2671
BcgI	CGANNNNNNTGC	2 cut(s)	2624, 2658
BciT130I	CCWGG	3 cut(s)	654, 1121, 2403
BclI	TGATCA	2 cut(s)	946, 1321
BcnI	CCSGG	4 cut(s)	264, 901, 902, 2028
BcoDI	GTCTC	3 cut(s)	152, 358, 553
BfaI	CTAG	5 cut(s)	936, 1020, 1392, 1955, 2061
BfmI	CTRYAG	1 cut(s)	956
BfoI	RGCGCY	1 cut(s)	1020
BfrI	CTTAAG	1 cut(s)	368
BfuAI	ACCTGC	3 cut(s)	1676, 2105, 2528
BglII	AGATCT	1 cut(s)	1802
BmcAI	AGTACT	1 cut(s)	104
Bme1390I	CCNGG	7 cut(s)	264, 654, 901, 902, 1121, 2028, 2403
Bme18I	GGWCC	6 cut(s)	373, 413, 1363, 1792, 1906, 2072
BmeRI	GACNNNNNGTC	1 cut(s)	1254
BmeT110I	CYCGRG	2 cut(s)	888, 900
BmgT120I	GGNCC	9 cut(s)	373, 413, 1164, 1363, 1792, 1906, 1933, 2025, 2072
BmrFI	CCNGG	7 cut(s)	264, 654, 901, 902, 1121, 2028, 2403
BmrI	ACTGGG	2 cut(s)	432, 791
BmuI	ACTGGG	2 cut(s)	432, 791
BpiI	GAAGAC	3 cut(s)	3, 12, 1530
BplI	GAGNNNNNCTC	2 cut(s)	2547, 2579
BpmI	CTGGAG	2 cut(s)	990, 1949
Bpu10I	CCTNAGC	2 cut(s)	1995, 2564
Bpu14I	TTCGAA	1 cut(s)	1880
BpuEI	CTTGAG	2 cut(s)	1545, 1806
BpuMI	CCSGG	4 cut(s)	264, 901, 902, 2028
BsaI	GGTCTC	1 cut(s)	152
BsaJI	CCNNGG	8 cut(s)	24, 422, 555, 652, 900, 901, 923, 2682
BsaWI	WCCGGW	1 cut(s)	90
BsaXI	ACNNNNNCTCC	8 cut(s)	139, 169, 178, 208, 713, 743, 1347, 1377
Bsc4I	CCNNNNNNNGG	9 cut(s)	77, 390, 782, 1652, 2022, 2063, 2064, 2351, 2451
Bse118I	RCCGGY	1 cut(s)	1235
Bse1I	ACTGG	9 cut(s)	100, 438, 514, 531, 786, 796, 935, 1446, 2220
Bse3DI	GCAATG	4 cut(s)	883, 2107, 2575, 2599
BseAI	TCCGGA	1 cut(s)	90
BseBI	CCWGG	3 cut(s)	654, 1121, 2403
BseDI	CCNNGG	8 cut(s)	24, 422, 555, 652, 900, 901, 923, 2682
BseGI	GGATG	6 cut(s)	230, 1645, 2036, 2049, 2395, 2669
BseLI	CCNNNNNNNGG	9 cut(s)	77, 390, 782, 1652, 2022, 2063, 2064, 2351, 2451
BseMI	GCAATG	4 cut(s)	883, 2107, 2575, 2599
BseNI	ACTGG	9 cut(s)	100, 438, 514, 531, 786, 796, 935, 1446, 2220
BseRI	GAGGAG	1 cut(s)	683
BseYI	CCCAGC	3 cut(s)	319, 699, 1028
BsgI	GTGCAG	1 cut(s)	1086
Bsh1236I	CGCG	1 cut(s)	2385
Bsh1285I	CGRYCG	1 cut(s)	121
BshNI	GGYRCC	1 cut(s)	484
BsiEI	CGRYCG	1 cut(s)	121
BsiHKAI	GWGCWC	1 cut(s)	114
BsiHKCI	CYCGRG	2 cut(s)	888, 900
BsiSI	CCGG	5 cut(s)	91, 264, 901, 1236, 2028
BslFI	GGGAC	1 cut(s)	1805
BslI	CCNNNNNNNGG	9 cut(s)	77, 390, 782, 1652, 2022, 2063, 2064, 2351, 2451
BsmAI	GTCTC	3 cut(s)	152, 358, 553
BsmFI	GGGAC	1 cut(s)	1805
BsmI	GAATGC	1 cut(s)	178
Bso31I	GGTCTC	1 cut(s)	152
BsoBI	CYCGRG	2 cut(s)	888, 900
Bsp119I	TTCGAA	1 cut(s)	1880
Bsp1286I	GDGCHC	1 cut(s)	114
Bsp13I	TCCGGA	1 cut(s)	90
Bsp19I	CCATGG	1 cut(s)	422
BspACI	CCGC	8 cut(s)	85, 211, 805, 874, 1033, 1861, 2146, 2418
BspEI	TCCGGA	1 cut(s)	90
BspFNI	CGCG	1 cut(s)	2385
BspHI	TCATGA	1 cut(s)	2620
BspMAI	CTGCAG	1 cut(s)	960
BspMI	ACCTGC	3 cut(s)	1676, 2105, 2528
BspQI	GCTCTTC	1 cut(s)	1758
BspT104I	TTCGAA	1 cut(s)	1880
BspT107I	GGYRCC	1 cut(s)	484
BspTI	CTTAAG	1 cut(s)	368
BspTNI	GGTCTC	1 cut(s)	152
BsrDI	GCAATG	4 cut(s)	883, 2107, 2575, 2599
BsrFI	RCCGGY	1 cut(s)	1235
BsrI	ACTGG	9 cut(s)	100, 438, 514, 531, 786, 796, 935, 1446, 2220
BssAI	RCCGGY	1 cut(s)	1235
BssECI	CCNNGG	8 cut(s)	24, 422, 555, 652, 900, 901, 923, 2682
BssSI	CACGAG	1 cut(s)	665
BssT1I	CCWWGG	3 cut(s)	24, 422, 555
Bst2BI	CACGAG	1 cut(s)	665
Bst2UI	CCWGG	3 cut(s)	654, 1121, 2403
Bst4CI	ACNGT	8 cut(s)	729, 741, 1481, 1600, 1873, 2116, 2163, 2639
Bst6I	CTCTTC	1 cut(s)	1758
BstAFI	CTTAAG	1 cut(s)	368
BstAPI	GCANNNNNTGC	1 cut(s)	2106
BstBI	TTCGAA	1 cut(s)	1880
BstC8I	GCNNGC	5 cut(s)	83, 110, 432, 1233, 1568
BstDSI	CCRYGG	2 cut(s)	422, 2682
BstEII	GGTNACC	1 cut(s)	1201
BstENI	CCTNNNNNAGG	1 cut(s)	2349
BstF5I	GGATG	6 cut(s)	230, 1645, 2036, 2049, 2395, 2669
BstFNI	CGCG	1 cut(s)	2385
BstH2I	RGCGCY	1 cut(s)	1020
BstHHI	GCGC	2 cut(s)	1019, 1734
BstMAI	GTCTC	3 cut(s)	152, 358, 553
BstMCI	CGRYCG	1 cut(s)	121
BstNI	CCWGG	3 cut(s)	654, 1121, 2403
BstNSI	RCATGY	2 cut(s)	288, 2094
BstPI	GGTNACC	1 cut(s)	1201
BstSCI	CCNGG	7 cut(s)	262, 652, 899, 900, 1119, 2026, 2401
BstSFI	CTRYAG	1 cut(s)	956
BstUI	CGCG	1 cut(s)	2385
BstV2I	GAAGAC	3 cut(s)	3, 12, 1530
BstX2I	RGATCY	8 cut(s)	259, 407, 755, 870, 1387, 1802, 2292, 2405
BstXI	CCANNNNNNTGG	2 cut(s)	1603, 1767
BstYI	RGATCY	8 cut(s)	259, 407, 755, 870, 1387, 1802, 2292, 2405
BtgI	CCRYGG	2 cut(s)	422, 2682
BtsCI	GGATG	6 cut(s)	230, 1645, 2036, 2049, 2395, 2669
BtsIMutI	CAGTG	6 cut(s)	538, 942, 1439, 1596, 1706, 2121
BveI	ACCTGC	3 cut(s)	1676, 2105, 2528
Cac8I	GCNNGC	5 cut(s)	83, 110, 432, 1233, 1568
CciI	TCATGA	1 cut(s)	2620
CfoI	GCGC	2 cut(s)	1019, 1734
Cfr10I	RCCGGY	1 cut(s)	1235
Cfr13I	GGNCC	9 cut(s)	373, 413, 1164, 1363, 1792, 1906, 1933, 2025, 2072
Cfr9I	CCCGGG	1 cut(s)	900
CseI	GACGC	2 cut(s)	1493, 2111
Csp6I	GTAC	5 cut(s)	103, 419, 746, 928, 2133
CviQI	GTAC	5 cut(s)	103, 419, 746, 928, 2133
DraI	TTTAAA	1 cut(s)	690
DriI	GACNNNNNGTC	1 cut(s)	1254
EaeI	YGGCCR	3 cut(s)	326, 806, 1233
Eam1104I	CTCTTC	1 cut(s)	1758
Eam1105I	GACNNNNNGTC	1 cut(s)	1254
EarI	CTCTTC	1 cut(s)	1758
EciI	GGCGGA	1 cut(s)	1048
Ecl136II	GAGCTC	1 cut(s)	112
Eco130I	CCWWGG	3 cut(s)	24, 422, 555
Eco147I	AGGCCT	3 cut(s)	886, 1073, 1566
Eco24I	GRGCYC	1 cut(s)	114
Eco31I	GGTCTC	1 cut(s)	152
Eco47I	GGWCC	6 cut(s)	373, 413, 1363, 1792, 1906, 2072
Eco47III	AGCGCT	1 cut(s)	1018
Eco53kI	GAGCTC	1 cut(s)	112
Eco57I	CTGAAG	1 cut(s)	1559
Eco88I	CYCGRG	2 cut(s)	888, 900
Eco91I	GGTNACC	1 cut(s)	1201
EcoICRI	GAGCTC	1 cut(s)	112
EcoNI	CCTNNNNNAGG	1 cut(s)	2349
EcoO109I	RGGNCCY	5 cut(s)	373, 1792, 1906, 1933, 2072
EcoO65I	GGTNACC	1 cut(s)	1201
EcoRI	GAATTC	1 cut(s)	1783
EcoRII	CCWGG	3 cut(s)	652, 1119, 2401
EcoT14I	CCWWGG	3 cut(s)	24, 422, 555
EcoT22I	ATGCAT	1 cut(s)	230
EcoT38I	GRGCYC	1 cut(s)	114
ErhI	CCWWGG	3 cut(s)	24, 422, 555
FalI	AAGNNNNNCTT	4 cut(s)	1554, 1586, 2111, 2143
FaqI	GGGAC	1 cut(s)	1805
FauI	CCCGC	2 cut(s)	204, 1868
FbaI	TGATCA	2 cut(s)	946, 1321
FblI	GTMKAC	2 cut(s)	380, 731
FokI	GGATG	6 cut(s)	237, 1652, 2036, 2043, 2402, 2656
FriOI	GRGCYC	1 cut(s)	114
FspBI	CTAG	5 cut(s)	936, 1020, 1392, 1955, 2061
GlaI	GCGC	2 cut(s)	1018, 1733
GsaI	CCCAGC	3 cut(s)	323, 703, 1032
GsuI	CTGGAG	2 cut(s)	990, 1949
HaeII	RGCGCY	1 cut(s)	1020
HapII	CCGG	5 cut(s)	91, 264, 901, 1236, 2028
HgaI	GACGC	2 cut(s)	1493, 2111
HhaI	GCGC	2 cut(s)	1019, 1734
Hin6I	GCGC	2 cut(s)	1017, 1732
HinP1I	GCGC	2 cut(s)	1017, 1732
HindIII	AAGCTT	1 cut(s)	521
HpaII	CCGG	5 cut(s)	91, 264, 901, 1236, 2028
HphI	GGTGA	9 cut(s)	67, 342, 872, 1250, 1618, 1700, 1985, 2334, 2391
Hpy166II	GTNNAC	6 cut(s)	381, 604, 732, 1770, 2578, 2602
Hpy8I	GTNNAC	6 cut(s)	381, 604, 732, 1770, 2578, 2602
Hpy99I	CGWCG	4 cut(s)	10, 19, 1678, 2127
HpyAV	CCTTC	9 cut(s)	137, 471, 712, 1094, 1265, 1534, 1607, 1885, 2062
HpyCH4III	ACNGT	8 cut(s)	729, 741, 1481, 1600, 1873, 2116, 2163, 2639
HpyCH4IV	ACGT	1 cut(s)	2259
HpySE526I	ACGT	1 cut(s)	2259
HspAI	GCGC	2 cut(s)	1017, 1732
KflI	GGGWCCC	1 cut(s)	1792
Kpn2I	TCCGGA	1 cut(s)	90
Ksp22I	TGATCA	2 cut(s)	946, 1321
LguI	GCTCTTC	1 cut(s)	1758
LmnI	GCTCC	6 cut(s)	93, 820, 1264, 1648, 1848, 1986
MaeI	CTAG	5 cut(s)	936, 1020, 1392, 1955, 2061
MaeII	ACGT	1 cut(s)	2259
MfeI	CAATTG	1 cut(s)	1226
MflI	RGATCY	8 cut(s)	259, 407, 755, 870, 1387, 1802, 2292, 2405
MhlI	GDGCHC	1 cut(s)	114
MlsI	TGGCCA	1 cut(s)	328
MluNI	TGGCCA	1 cut(s)	328
MlyI	GAGTC	4 cut(s)	206, 388, 637, 1985
MmeI	TCCRAC	2 cut(s)	164, 237
Mox20I	TGGCCA	1 cut(s)	328
Mph1103I	ATGCAT	1 cut(s)	230
MroI	TCCGGA	1 cut(s)	90
MroXI	GAANNNNTTC	1 cut(s)	1300
MscI	TGGCCA	1 cut(s)	328
MseI	TTAA	8 cut(s)	369, 516, 525, 660, 689, 2111, 2138, 2319
MslI	CAYNNNNRTG	3 cut(s)	171, 1601, 1765
Msp20I	TGGCCA	1 cut(s)	328
MspA1I	CMGCKG	1 cut(s)	1863
MspCI	CTTAAG	1 cut(s)	368
MspI	CCGG	5 cut(s)	91, 264, 901, 1236, 2028
MspR9I	CCNGG	7 cut(s)	264, 654, 901, 902, 1121, 2028, 2403
MunI	CAATTG	1 cut(s)	1226
Mva1269I	GAATGC	1 cut(s)	178
MvaI	CCWGG	3 cut(s)	654, 1121, 2403
MvnI	CGCG	1 cut(s)	2385
NciI	CCSGG	4 cut(s)	264, 901, 902, 2028
NcoI	CCATGG	1 cut(s)	422
NmuCI	GTSAC	5 cut(s)	239, 1238, 1246, 2397, 2514
NsiI	ATGCAT	1 cut(s)	230
NspI	RCATGY	2 cut(s)	288, 2094
NspV	TTCGAA	1 cut(s)	1880
OliI	CACNNNNGTG	1 cut(s)	1601
PaeR7I	CTCGAG	1 cut(s)	888
PagI	TCATGA	1 cut(s)	2620
PaqCI	CACCTGC	3 cut(s)	1676, 2105, 2528
PceI	AGGCCT	3 cut(s)	886, 1073, 1566
PciI	ACATGT	1 cut(s)	284
PciSI	GCTCTTC	1 cut(s)	1758
PcsI	WCGNNNNNNNCGW	3 cut(s)	11, 14, 68
PctI	GAATGC	1 cut(s)	178
PdmI	GAANNNNTTC	1 cut(s)	1300
PfeI	GAWTC	6 cut(s)	150, 851, 939, 1124, 1696, 2411
PflMI	CCANNNNNTGG	2 cut(s)	390, 1652
PfoI	TCCNGGA	2 cut(s)	262, 1119
Ple19I	CGATCG	1 cut(s)	121
PleI	GAGTC	4 cut(s)	205, 388, 636, 1984
PpsI	GAGTC	4 cut(s)	205, 388, 636, 1984
PpuMI	RGGWCCY	4 cut(s)	373, 1792, 1906, 2072
PscI	ACATGT	1 cut(s)	284
PshBI	ATTAAT	1 cut(s)	2138
PsiI	TTATAA	1 cut(s)	834
Psp124BI	GAGCTC	1 cut(s)	114
Psp5II	RGGWCCY	4 cut(s)	373, 1792, 1906, 2072
Psp6I	CCWGG	3 cut(s)	652, 1119, 2401
PspEI	GGTNACC	1 cut(s)	1201
PspFI	CCCAGC	3 cut(s)	319, 699, 1028
PspGI	CCWGG	3 cut(s)	652, 1119, 2401
PspPI	GGNCC	9 cut(s)	373, 413, 1164, 1363, 1792, 1906, 1933, 2025, 2072
PspPPI	RGGWCCY	4 cut(s)	373, 1792, 1906, 2072
PspXI	VCTCGAGB	1 cut(s)	888
PsrI	GAACNNNNNNTAC	2 cut(s)	1474, 1506
PstI	CTGCAG	1 cut(s)	960
PsuI	RGATCY	8 cut(s)	259, 407, 755, 870, 1387, 1802, 2292, 2405
PvuI	CGATCG	1 cut(s)	121
RsaI	GTAC	5 cut(s)	104, 420, 747, 929, 2134
RsaNI	GTAC	5 cut(s)	103, 419, 746, 928, 2133
RseI	CAYNNNNRTG	3 cut(s)	171, 1601, 1765
SacI	GAGCTC	1 cut(s)	114
SapI	GCTCTTC	1 cut(s)	1758
SaqAI	TTAA	8 cut(s)	369, 516, 525, 660, 689, 2111, 2138, 2319
Sau96I	GGNCC	9 cut(s)	373, 413, 1164, 1363, 1792, 1906, 1933, 2025, 2072
ScaI	AGTACT	1 cut(s)	104
SchI	GAGTC	4 cut(s)	206, 388, 637, 1985
ScrFI	CCNGG	7 cut(s)	264, 654, 901, 902, 1121, 2028, 2403
SduI	GDGCHC	1 cut(s)	114
SfcI	CTRYAG	1 cut(s)	956
Sfr274I	CTCGAG	1 cut(s)	888
SfuI	TTCGAA	1 cut(s)	1880
SinI	GGWCC	6 cut(s)	373, 413, 1363, 1792, 1906, 2072
SlaI	CTCGAG	1 cut(s)	888
SmaI	CCCGGG	1 cut(s)	902
SmiMI	CAYNNNNRTG	3 cut(s)	171, 1601, 1765
SmlI	CTYRAG	4 cut(s)	368, 888, 1560, 1821
SmoI	CTYRAG	4 cut(s)	368, 888, 1560, 1821
SseBI	AGGCCT	3 cut(s)	886, 1073, 1566
SsiI	CCGC	8 cut(s)	85, 211, 805, 874, 1033, 1861, 2146, 2418
SspI	AATATT	1 cut(s)	543
SspMI	CTAG	5 cut(s)	936, 1020, 1392, 1955, 2061
SstI	GAGCTC	1 cut(s)	114
StuI	AGGCCT	3 cut(s)	886, 1073, 1566
StyD4I	CCNGG	7 cut(s)	262, 652, 899, 900, 1119, 2026, 2401
StyI	CCWWGG	3 cut(s)	24, 422, 555
TaaI	ACNGT	8 cut(s)	729, 741, 1481, 1600, 1873, 2116, 2163, 2639
TaiI	ACGT	1 cut(s)	2262
TaqI	TCGA	6 cut(s)	889, 1002, 1258, 1880, 2583, 2607
TaqII	GACCGA	1 cut(s)	1351
TatI	WGTACW	1 cut(s)	102
TauI	GCSGC	4 cut(s)	88, 808, 2149, 2421
TfiI	GAWTC	6 cut(s)	150, 851, 939, 1124, 1696, 2411
Tru1I	TTAA	8 cut(s)	369, 516, 525, 660, 689, 2111, 2138, 2319
Tru9I	TTAA	8 cut(s)	369, 516, 525, 660, 689, 2111, 2138, 2319
TscAI	CASTG	6 cut(s)	538, 949, 1446, 1603, 1706, 2121
TseFI	GTSAC	5 cut(s)	239, 1238, 1246, 2397, 2514
Tsp45I	GTSAC	5 cut(s)	239, 1238, 1246, 2397, 2514
TspGWI	ACGGA	3 cut(s)	1368, 1850, 2120
TspMI	CCCGGG	1 cut(s)	900
TspRI	CASTG	6 cut(s)	538, 949, 1446, 1603, 1706, 2121
Van91I	CCANNNNNTGG	2 cut(s)	390, 1652
Vha464I	CTTAAG	1 cut(s)	368
VpaK11BI	GGWCC	6 cut(s)	373, 413, 1363, 1792, 1906, 2072
VspI	ATTAAT	1 cut(s)	2138
XagI	CCTNNNNNAGG	1 cut(s)	2349
XapI	RAATTY	3 cut(s)	578, 979, 1783
XceI	RCATGY	2 cut(s)	288, 2094
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1228, 2060
XhoI	CTCGAG	1 cut(s)	888
XmaI	CCCGGG	1 cut(s)	900
XmiI	GTMKAC	2 cut(s)	380, 731
XmnI	GAANNNNTTC	1 cut(s)	1300
XspI	CTAG	5 cut(s)	936, 1020, 1392, 1955, 2061
ZrmI	AGTACT	1 cut(s)	104
Zsp2I	ATGCAT	1 cut(s)	230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.