Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0005133

Physical Location & Seq

Genomic Coordinates Forward (+)

4786587 .. 4794240

Gene Structure 7654 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031400

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2715 bp

ATGTCGCCGTCGTCGTCGTCTTCTTCTTCCAAGTGCTGCTACAATTCCGAGTGCGGCGAATTGAAGCCGGAGCACCCGAGGAAGGCCTGGCGCCTCCGCACCGGAGACTTCGCCGAGCTCTGCGATCGATGCGCTCTCATTTATGAAGAAGGAAGATTTTGTGAGACTTTTCATGTGAAAGCTTCTGGGTGGAGGTGTTGTGAGTCGTGTGGAAAGCGGGTTCATTGTGGATGTATCGTTTCAGCTCAAGCCTTTACCTTGTTGGATCCTGGAGGGATTGAGTGCGTAACATGTGCCAGGAAACATATTGTTTTGTCATCAAATCCAGCATGGGCACCAACTTTGTTCTTTCATTCACCTTTATCTGATAGACTGAAGGACATGTCTGGCAAAAATTGGACTCAGTTGGCTGGATCTGGTCCTGTACCATGGAGGCAAGCACCCAGTTTATTCAATTCTTCAATATCTTCATCTGAGTTGCATGGGAGGTTGCCATATGATGTTGATGGTTCAATGGGAATTAATAAACTTAATGCCAGTGAAAGAGTATCAGTCCATCCCTTGGAAAAGAAGAAAGCTGAGGATTTCTCTGAAAGATTAATGAAAGTGAACCTTGCAATTGGCACACCAGTTAGTTTTGAAAATGGGAATTCAGGAAATAAATGCATGGAGCAAAGCAGTTCATGTTTAAATATATCTCAACAGCCTGCCTCTTTGAAGGAGGAACCATCTACTCCACAGTTTGTCCTGTCTGTACCTTATGTATCAGCAAGTGAAACAAATGGTCAAACAAACGGTCAAGTGGTAGTTACGGGAACCAATTTGCGGCCTGCCCTTCCTATGGTAAAGCAGTTCAATGGTACTTTACATAATGGAGTTGAATTGTCTGGTGATACTCAGATCCGCAATGCAAGGCCTCGAGCAGATGCACGGGGAAGAAGTCCGTTACTTCCACGGTACTGGCCTAGATTTACTGATCAAGAGCTACAGCAAATGTCTGGAGATTCAAATTCTGTCATTACTCCATTATTTGAGAAAATGCTGAGCGCAAGTGATGCTGGGAGGATTGGACGTCTGGTGTTGCCAAAAAAGTGTGCAGAGGCCTACTTCCCACCGATTTCTCAGCCTGAAGGATTACCGCTTAAAGTCCAAGATTCGAAAGGCAAGGAATGGGTATTTCAGTTTCGCTTCTGGCCCAACAATAATAGCAGAATGTATGTTTTGGAGGGGGTTACTCCTTGCATACAATCCATGCAATTGCAAGCAGGCGACATAGTGACATTCAGTCGATTAGAGCCCGGAGGGAAATTGGTTATGGGGTGCAGAAGGGCTTCAACTGCCCCATCTGATCAGGAAAATGATACAAACAAGATGGGGAATGGAGTTTCAGCCCATGTAGATGTTAGTATAACAGATACCAAATCTGGTGAAGTTTTTTCAAATGCTGAATTGGCAGATCCTAGTACATTTCCCAAAGGTGATAAGTCAAGTTACATATCAAAAGAAGTAGAGGGAGCTAAATCTTTGATCTCTCGAAAAAGGAAAAATAGCAACTTGGGGTCTAAGAGCAAGCGCCTGAGAATTGAAAAGGAAGATCTGATAGAGCTGAAGATAACATGGGAAGAAGCTCAAGGACTGCTTCGCCCTCCTCCTAACCATATTCCTACTGTTGTGGTGATTGAAGGTTTTGAGTTTGAGGAATATGAGGATGGTCCAATTCTTGGGAAGCCGACACTTCATGCTACAGGAAGTAAAGGTGAGAAGATTCAATGGGCACAATGTGAAGTTTGTTGTAAGTGGCGCCAATTGCCTGTTAATGCCCTTCTTCCCTCAAAGTGGACTTGCTCAGATAACTCATGGGACCCTGATAGGTCATTGTGTTCAGCAGCTCAAGAGTTGACAACAGAACAGCTTGAAGATCTGCTTCCCAGTTGTAATTCAGTTGCTCCAAAGAAAATGAAGGCTACTAAACAGGACCCAGATAACATTGAAGCTTTGGAGGGGCTTGATACACTTGCAAACCTAGCTATCTTGGGAGAAGGTGAGTCACTCCCAGCATCAGCTCAGGCCACAACAAAGCACCCCCGACATAGGCCCGGATGCTCATGCATTGTTTGCATCCAGCCCCCTAGTGGGAAGGGTCCTAAACACAAGCAAACGTGCATCTGCAATGTATGCTCAACAGTGAAGCGCCGGTTCCGTACGTTAATGATGCGGCGCGAGAAGAATAAGTCGGAGAAAGAAGCAGAAACTACACGCAAAAAGCAGCAGCAGCAACAGCAATTACTAGACGATGAGACCCTCTTATGTGGTAACGCAGGAAGTAGCAGTCCAAACAACAAAGTGGAGGTCCTTGAGCTTTCTGATGATGACTTTAACAGGACGAAATCTCCAACTCCACCTTATAAAGGCCAGATTGATCTGAATATCCAGCCAGAGCGGGAGGAAGATCTATCACCTGGCTCAGATTCTGGTGGCGTGATAAAGTTGCTCCAAGATGCCACAGAAAGGTATCTCAGGCAGCAGAGGCAGAGTTTGTCAACCTCTGGTGGTGGTGAAAGATCCATGGGCAACAAGTCGCAGCGAGCTGGAGGTGAAGCAGGGGGAGAACCACTTGGCAACATTGCAATTGTTGGGAATAGCAATCATGATAGTGGCAATGATCATCCTGCAACTTTATCTATCAAAGCTTCAGCTTCCATGACAGCTACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B3 zf-CW

Protein Analysis

905

Amino Acids

98.96

Weight (kDa)

7.97

Isoelectric Point (pI)

50.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf_VAL1_N	PF25813	56 - 103	5.1e-22	VAL1-like, N-terminal zinc finger domain
B3	PF02362	344 - 444	2.8e-16	B3 DNA binding domain
zf-CW	PF07496	590 - 632	2.7e-09	CW-type Zinc Finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21550 AT4G21550 AT4G21550
fragaria_vesca	FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120
malus_domestica	MD05G1324700.v1.1 MD10G1302500.v1.1
prunus_persica	Prupe.4G036100_v2.0.a1
pyrus_communis	pycom05g29890 pycom10g25500
rosa_chinensis	RchiOBHm_Chr5g0006571
rosa_laevigata	RLG00000031400
rosa_multiflora	Rmu_sc0007807.1_g000001 Rmu_sc0011154.1_g000008 Rmu_sc0011154.1_g000009
rosa_roxburghii	Rroxscaffold_1G00069470
rosa_rugosa	Rorug04G0422600.1 Rorug04G0422700
rosa_samantha	Rh5AG049800 Rh5BG049100 Rh5CG057400 Rh5DG048500
rosa_wichuraiana	Rw5G004990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2406
AatII	GACGTC	1 cut(s)	1075
AccB1I	GGYRCC	3 cut(s)	90, 334, 1800
AccB7I	CCANNNNNTGG	2 cut(s)	562, 1721
AccBSI	CCGCTC	1 cut(s)	2440
AccII	CGCG	1 cut(s)	2220
AciI	CCGC	8 cut(s)	54, 97, 217, 826, 904, 1139, 2215, 2440
AclWI	GGATC	6 cut(s)	260, 273, 421, 895, 1451, 2556
AcsI	RAATTY	2 cut(s)	649, 1009
AcuI	CTGAAG	4 cut(s)	395, 1149, 1628, 2676
AcyI	GRCGYC	3 cut(s)	91, 1072, 1801
AdeI	CACNNNGTG	1 cut(s)	1781
AfaI	GTAC	6 cut(s)	426, 756, 862, 959, 1465, 2203
AflIII	ACRYGT	2 cut(s)	290, 381
AhdI	GACNNNNNGTC	1 cut(s)	1284
AjnI	CCWGG	4 cut(s)	86, 268, 296, 2458
AloI	GAACNNNNNNTCC	2 cut(s)	204, 236
Alw21I	GWGCWC	2 cut(s)	75, 120
Alw26I	GTCTC	3 cut(s)	99, 158, 2291
AlwI	GGATC	6 cut(s)	260, 273, 421, 895, 1451, 2556
AlwNI	CAGNNNCTG	1 cut(s)	2471
Ama87I	CYCGRG	2 cut(s)	76, 918
AoxI	GGCC	9 cut(s)	84, 827, 914, 962, 1101, 1193, 2067, 2093, 2410
ApeKI	GCWGC	7 cut(s)	36, 1886, 2266, 2269, 2272, 2521, 2581
ApoI	RAATTY	2 cut(s)	649, 1009
ArsI	GACNNNNNNTTYG	4 cut(s)	781, 813, 2334, 2366
AseI	ATTAAT	2 cut(s)	522, 599
Asp700I	GAANNNNTTC	1 cut(s)	1330
AspLEI	GCGC	7 cut(s)	93, 134, 1049, 1575, 1803, 2193, 2220
AspS9I	GGNCC	8 cut(s)	419, 1194, 1712, 1861, 1975, 2094, 2141, 2350
AsuC2I	CCSGG	2 cut(s)	1299, 2097
AsuII	TTCGAA	1 cut(s)	1157
AvaI	CYCGRG	2 cut(s)	76, 918
AvaII	GGWCC	6 cut(s)	419, 1712, 1861, 1975, 2141, 2350
BaeGI	GKGCMC	2 cut(s)	337, 1777
BamHI	GGATCC	1 cut(s)	265
BanI	GGYRCC	3 cut(s)	90, 334, 1800
BanII	GRGCYC	2 cut(s)	120, 1299
BbsI	GAAGAC	1 cut(s)	12
Bbv12I	GWGCWC	2 cut(s)	75, 120
BbvCI	CCTCAGC	1 cut(s)	579
BbvI	GCAGC	7 cut(s)	23, 1898, 2278, 2281, 2284, 2533, 2593
BccI	CCATC	6 cut(s)	500, 564, 736, 1351, 1366, 1703
BciT130I	CCWGG	4 cut(s)	88, 270, 298, 2460
BclI	TGATCA	3 cut(s)	976, 1348, 2662
BcnI	CCSGG	2 cut(s)	1299, 2097
BcoDI	GTCTC	3 cut(s)	99, 158, 2291
BfaI	CTAG	5 cut(s)	966, 1461, 2024, 2130, 2288
BfmI	CTRYAG	2 cut(s)	986, 1743
BfoI	RGCGCY	4 cut(s)	94, 1576, 1804, 2194
BglII	AGATCT	3 cut(s)	1594, 1918, 2449
BlpI	GCTNAGC	1 cut(s)	1043
Bme1390I	CCNGG	6 cut(s)	88, 270, 298, 1299, 2097, 2460
Bme18I	GGWCC	6 cut(s)	419, 1712, 1861, 1975, 2141, 2350
BmeRI	GACNNNNNGTC	1 cut(s)	1284
BmeT110I	CYCGRG	2 cut(s)	76, 918
BmgT120I	GGNCC	8 cut(s)	419, 1194, 1712, 1861, 1975, 2094, 2141, 2350
BmrFI	CCNGG	6 cut(s)	88, 270, 298, 1299, 2097, 2460
BmrI	ACTGGG	2 cut(s)	438, 1923
BmsI	GCATC	9 cut(s)	119, 916, 1045, 2066, 2090, 2127, 2172, 2202, 2488
BmuI	ACTGGG	2 cut(s)	438, 1923
BpiI	GAAGAC	1 cut(s)	12
BplI	GAGNNNNNCTC	2 cut(s)	572, 604
BpmI	CTGGAG	3 cut(s)	291, 1020, 2610
Bpu10I	CCTNAGC	2 cut(s)	579, 2064
Bpu1102I	GCTNAGC	1 cut(s)	1043
Bpu14I	TTCGAA	1 cut(s)	1157
BpuEI	CTTGAG	4 cut(s)	231, 1614, 1875, 2375
BpuMI	CCSGG	2 cut(s)	1299, 2097
Bsa29I	ATCGAT	1 cut(s)	127
BsaAI	YACGTR	1 cut(s)	2712
BsaHI	GRCGYC	3 cut(s)	91, 1072, 1801
BsaI	GGTCTC	1 cut(s)	2291
BsaJI	CCNNGG	5 cut(s)	77, 428, 561, 953, 2565
BsaWI	WCCGGW	1 cut(s)	101
BsaXI	ACNNNNNCTCC	4 cut(s)	184, 214, 2374, 2404
Bse118I	RCCGGY	1 cut(s)	2193
Bse1I	ACTGG	5 cut(s)	444, 537, 629, 965, 1929
Bse3DI	GCAATG	4 cut(s)	913, 2176, 2622, 2665
BseBI	CCWGG	4 cut(s)	88, 270, 298, 2460
BseCI	ATCGAT	1 cut(s)	127
BseDI	CCNNGG	5 cut(s)	77, 428, 561, 953, 2565
BseGI	GGATG	6 cut(s)	236, 556, 1714, 2105, 2118, 2665
BseMI	GCAATG	4 cut(s)	913, 2176, 2622, 2665
BseNI	ACTGG	5 cut(s)	444, 537, 629, 965, 1929
BseRI	GAGGAG	1 cut(s)	1638
BseSI	GKGCMC	2 cut(s)	337, 1777
BseXI	GCAGC	7 cut(s)	23, 1898, 2278, 2281, 2284, 2533, 2593
BseYI	CCCAGC	2 cut(s)	1058, 2053
BsgI	GTGCAG	2 cut(s)	1116, 1342
Bsh1236I	CGCG	1 cut(s)	2220
Bsh1285I	CGRYCG	1 cut(s)	127
BshFI	GGCC	9 cut(s)	86, 829, 916, 964, 1103, 1195, 2069, 2095, 2412
BshNI	GGYRCC	3 cut(s)	90, 334, 1800
BshVI	ATCGAT	1 cut(s)	127
BsiEI	CGRYCG	1 cut(s)	127
BsiHKAI	GWGCWC	2 cut(s)	75, 120
BsiHKCI	CYCGRG	2 cut(s)	76, 918
BsiSI	CCGG	5 cut(s)	68, 102, 1299, 2097, 2194
BsiWI	CGTACG	1 cut(s)	2201
BslFI	GGGAC	1 cut(s)	1874
BsmAI	GTCTC	3 cut(s)	99, 158, 2291
BsmFI	GGGAC	1 cut(s)	1874
BsnI	GGCC	9 cut(s)	86, 829, 916, 964, 1103, 1195, 2069, 2095, 2412
Bso31I	GGTCTC	1 cut(s)	2291
BsoBI	CYCGRG	2 cut(s)	76, 918
Bsp119I	TTCGAA	1 cut(s)	1157
Bsp1286I	GDGCHC	5 cut(s)	75, 120, 337, 1299, 1777
Bsp1720I	GCTNAGC	1 cut(s)	1043
Bsp19I	CCATGG	2 cut(s)	428, 2565
BspACI	CCGC	8 cut(s)	54, 97, 217, 826, 904, 1139, 2215, 2440
BspANI	GGCC	9 cut(s)	86, 829, 916, 964, 1103, 1195, 2069, 2095, 2412
BspDI	ATCGAT	1 cut(s)	127
BspFNI	CGCG	1 cut(s)	2220
BspHI	TCATGA	1 cut(s)	2647
BspPI	GGATC	6 cut(s)	260, 273, 421, 895, 1451, 2556
BspT104I	TTCGAA	1 cut(s)	1157
BspT107I	GGYRCC	3 cut(s)	90, 334, 1800
BspTNI	GGTCTC	1 cut(s)	2291
BsrBI	CCGCTC	1 cut(s)	2440
BsrDI	GCAATG	4 cut(s)	913, 2176, 2622, 2665
BsrFI	RCCGGY	1 cut(s)	2193
BsrI	ACTGG	5 cut(s)	444, 537, 629, 965, 1929
BssAI	RCCGGY	1 cut(s)	2193
BssECI	CCNNGG	5 cut(s)	77, 428, 561, 953, 2565
BssNI	GRCGYC	3 cut(s)	91, 1072, 1801
BssT1I	CCWWGG	3 cut(s)	428, 561, 2565
Bst2UI	CCWGG	4 cut(s)	88, 270, 298, 2460
Bst4CI	ACNGT	5 cut(s)	741, 797, 957, 1669, 2185
BstACI	GRCGYC	3 cut(s)	91, 1072, 1801
BstAPI	GCANNNNNTGC	3 cut(s)	1055, 2115, 2175
BstBAI	YACGTR	1 cut(s)	2712
BstBI	TTCGAA	1 cut(s)	1157
BstC8I	GCNNGC	7 cut(s)	438, 708, 831, 1263, 1267, 1571, 2586
BstDSI	CCRYGG	3 cut(s)	428, 953, 2565
BstENI	CCTNNNNNAGG	1 cut(s)	2406
BstF5I	GGATG	6 cut(s)	236, 556, 1714, 2105, 2118, 2665
BstFNI	CGCG	1 cut(s)	2220
BstH2I	RGCGCY	4 cut(s)	94, 1576, 1804, 2194
BstHHI	GCGC	7 cut(s)	93, 134, 1049, 1575, 1803, 2193, 2220
BstMAI	GTCTC	3 cut(s)	99, 158, 2291
BstMCI	CGRYCG	1 cut(s)	127
BstNI	CCWGG	4 cut(s)	88, 270, 298, 2460
BstNSI	RCATGY	2 cut(s)	294, 385
BstSCI	CCNGG	6 cut(s)	86, 268, 296, 1297, 2095, 2458
BstSFI	CTRYAG	2 cut(s)	986, 1743
BstSLI	GKGCMC	2 cut(s)	337, 1777
BstUI	CGCG	1 cut(s)	2220
BstV1I	GCAGC	7 cut(s)	23, 1898, 2278, 2281, 2284, 2533, 2593
BstV2I	GAAGAC	1 cut(s)	12
BstX2I	RGATCY	8 cut(s)	265, 413, 900, 1456, 1594, 1918, 2449, 2561
BstXI	CCANNNNNNTGG	1 cut(s)	960
BstYI	RGATCY	8 cut(s)	265, 413, 900, 1456, 1594, 1918, 2449, 2561
Bsu15I	ATCGAT	1 cut(s)	127
BsuRI	GGCC	9 cut(s)	86, 829, 916, 964, 1103, 1195, 2069, 2095, 2412
BsuTUI	ATCGAT	1 cut(s)	127
BtgI	CCRYGG	3 cut(s)	428, 953, 2565
BtsCI	GGATG	6 cut(s)	236, 556, 1714, 2105, 2118, 2665
BtsIMutI	CAGTG	2 cut(s)	544, 2190
Cac8I	GCNNGC	7 cut(s)	438, 708, 831, 1263, 1267, 1571, 2586
CaiI	CAGNNNCTG	1 cut(s)	2471
CciI	TCATGA	1 cut(s)	2647
CfoI	GCGC	7 cut(s)	93, 134, 1049, 1575, 1803, 2193, 2220
Cfr10I	RCCGGY	1 cut(s)	2193
Cfr13I	GGNCC	8 cut(s)	419, 1194, 1712, 1861, 1975, 2094, 2141, 2350
ClaI	ATCGAT	1 cut(s)	127
Csp6I	GTAC	6 cut(s)	425, 755, 861, 958, 1464, 2202
CviQI	GTAC	6 cut(s)	425, 755, 861, 958, 1464, 2202
DinI	GGCGCC	2 cut(s)	92, 1802
DraI	TTTAAA	1 cut(s)	690
DraIII	CACNNNGTG	1 cut(s)	1781
DriI	GACNNNNNGTC	1 cut(s)	1284
Eam1105I	GACNNNNNGTC	1 cut(s)	1284
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	3 cut(s)	428, 561, 2565
Eco147I	AGGCCT	3 cut(s)	86, 916, 1103
Eco24I	GRGCYC	2 cut(s)	120, 1299
Eco31I	GGTCTC	1 cut(s)	2291
Eco47I	GGWCC	6 cut(s)	419, 1712, 1861, 1975, 2141, 2350
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	4 cut(s)	395, 1149, 1628, 2676
Eco88I	CYCGRG	2 cut(s)	76, 918
EcoICRI	GAGCTC	1 cut(s)	118
EcoNI	CCTNNNNNAGG	1 cut(s)	2406
EcoO109I	RGGNCCY	4 cut(s)	1861, 1975, 2141, 2350
EcoRI	GAATTC	1 cut(s)	649
EcoRII	CCWGG	4 cut(s)	86, 268, 296, 2458
EcoT14I	CCWWGG	3 cut(s)	428, 561, 2565
EcoT22I	ATGCAT	2 cut(s)	668, 2111
EcoT38I	GRGCYC	2 cut(s)	120, 1299
EgeI	GGCGCC	2 cut(s)	92, 1802
EheI	GGCGCC	2 cut(s)	92, 1802
ErhI	CCWWGG	3 cut(s)	428, 561, 2565
FalI	AAGNNNNNCTT	6 cut(s)	597, 629, 1623, 1655, 1908, 1940
FaqI	GGGAC	1 cut(s)	1874
FauI	CCCGC	2 cut(s)	210, 2433
FauNDI	CATATG	1 cut(s)	496
FbaI	TGATCA	3 cut(s)	976, 1348, 2662
FokI	GGATG	6 cut(s)	243, 543, 1721, 2105, 2112, 2652
FriOI	GRGCYC	2 cut(s)	120, 1299
FspBI	CTAG	5 cut(s)	966, 1461, 2024, 2130, 2288
GlaI	GCGC	7 cut(s)	92, 133, 1048, 1574, 1802, 2192, 2219
GsaI	CCCAGC	2 cut(s)	1062, 2057
GsuI	CTGGAG	3 cut(s)	291, 1020, 2610
HaeII	RGCGCY	4 cut(s)	94, 1576, 1804, 2194
HaeIII	GGCC	9 cut(s)	86, 829, 916, 964, 1103, 1195, 2069, 2095, 2412
HapII	CCGG	5 cut(s)	68, 102, 1299, 2097, 2194
HhaI	GCGC	7 cut(s)	93, 134, 1049, 1575, 1803, 2193, 2220
Hin1I	GRCGYC	3 cut(s)	91, 1072, 1801
Hin6I	GCGC	7 cut(s)	91, 132, 1047, 1573, 1801, 2191, 2218
HinP1I	GCGC	7 cut(s)	91, 132, 1047, 1573, 1801, 2191, 2218
HincII	GTYRAC	2 cut(s)	1899, 2541
HindII	GTYRAC	2 cut(s)	1899, 2541
HindIII	AAGCTT	3 cut(s)	180, 1992, 2688
HinfI	GANTC	7 cut(s)	203, 400, 1004, 1154, 1765, 2045, 2468
HpaII	CCGG	5 cut(s)	68, 102, 1299, 2097, 2194
Hpy166II	GTNNAC	4 cut(s)	610, 1839, 1899, 2541
Hpy188III	TCNNGA	7 cut(s)	654, 980, 999, 1352, 1533, 1892, 2648
Hpy8I	GTNNAC	4 cut(s)	610, 1839, 1899, 2541
Hpy99I	CGWCG	3 cut(s)	13, 16, 19
HpyCH4III	ACNGT	5 cut(s)	741, 797, 957, 1669, 2185
HpyCH4IV	ACGT	4 cut(s)	1072, 2159, 2204, 2711
HpySE526I	ACGT	4 cut(s)	1072, 2159, 2204, 2711
Hsp92I	GRCGYC	3 cut(s)	91, 1072, 1801
HspAI	GCGC	7 cut(s)	91, 132, 1047, 1573, 1801, 2191, 2218
KasI	GGCGCC	2 cut(s)	90, 1800
KflI	GGGWCCC	1 cut(s)	1861
Ksp22I	TGATCA	3 cut(s)	976, 1348, 2662
LmnI	GCTCC	5 cut(s)	70, 670, 1514, 1951, 2496
Lsp1109I	GCAGC	7 cut(s)	23, 1898, 2278, 2281, 2284, 2533, 2593
LweI	GCATC	9 cut(s)	119, 916, 1045, 2066, 2090, 2127, 2172, 2202, 2488
MaeI	CTAG	5 cut(s)	966, 1461, 2024, 2130, 2288
MaeII	ACGT	4 cut(s)	1072, 2159, 2204, 2711
MaeIII	GTNAC	8 cut(s)	286, 808, 945, 1231, 1276, 1490, 2046, 2312
MbiI	CCGCTC	1 cut(s)	2440
MfeI	CAATTG	4 cut(s)	618, 1256, 1805, 2628
MflI	RGATCY	8 cut(s)	265, 413, 900, 1456, 1594, 1918, 2449, 2561
MhlI	GDGCHC	5 cut(s)	75, 120, 337, 1299, 1777
Mly113I	GGCGCC	2 cut(s)	91, 1801
MlyI	GAGTC	3 cut(s)	212, 394, 2054
MmeI	TCCRAC	3 cut(s)	243, 2214, 2417
Mph1103I	ATGCAT	2 cut(s)	668, 2111
MroXI	GAANNNNTTC	1 cut(s)	1330
MseI	TTAA	8 cut(s)	522, 531, 599, 689, 1143, 1815, 2207, 2376
MslI	CAYNNNNRTG	2 cut(s)	1398, 2652
MspI	CCGG	5 cut(s)	68, 102, 1299, 2097, 2194
MspR9I	CCNGG	6 cut(s)	88, 270, 298, 1299, 2097, 2460
MunI	CAATTG	4 cut(s)	618, 1256, 1805, 2628
MvaI	CCWGG	4 cut(s)	88, 270, 298, 2460
MvnI	CGCG	1 cut(s)	2220
NarI	GGCGCC	2 cut(s)	91, 1801
NciI	CCSGG	2 cut(s)	1299, 2097
NcoI	CCATGG	2 cut(s)	428, 2565
NdeI	CATATG	1 cut(s)	496
NmeAIII	GCCGAG	1 cut(s)	139
NmuCI	GTSAC	2 cut(s)	1276, 2046
NsiI	ATGCAT	2 cut(s)	668, 2111
NspI	RCATGY	2 cut(s)	294, 385
NspV	TTCGAA	1 cut(s)	1157
PaeR7I	CTCGAG	1 cut(s)	918
PagI	TCATGA	1 cut(s)	2647
PceI	AGGCCT	3 cut(s)	86, 916, 1103
PciI	ACATGT	2 cut(s)	290, 381
PcsI	WCGNNNNNNNCGW	1 cut(s)	11
PdmI	GAANNNNTTC	1 cut(s)	1330
PfeI	GAWTC	4 cut(s)	1004, 1154, 1765, 2468
Pfl23II	CGTACG	1 cut(s)	2201
PflMI	CCANNNNNTGG	2 cut(s)	562, 1721
PfoI	TCCNGGA	1 cut(s)	268
Ple19I	CGATCG	1 cut(s)	127
PleI	GAGTC	3 cut(s)	211, 394, 2053
PluTI	GGCGCC	2 cut(s)	94, 1804
PpsI	GAGTC	3 cut(s)	211, 394, 2053
Ppu21I	YACGTR	1 cut(s)	2712
PpuMI	RGGWCCY	4 cut(s)	1861, 1975, 2141, 2350
PscI	ACATGT	2 cut(s)	290, 381
PshBI	ATTAAT	2 cut(s)	522, 599
PsiI	TTATAA	1 cut(s)	2406
Psp124BI	GAGCTC	1 cut(s)	120
Psp5II	RGGWCCY	4 cut(s)	1861, 1975, 2141, 2350
Psp6I	CCWGG	4 cut(s)	86, 268, 296, 2458
PspFI	CCCAGC	2 cut(s)	1058, 2053
PspGI	CCWGG	4 cut(s)	86, 268, 296, 2458
PspLI	CGTACG	1 cut(s)	2201
PspPI	GGNCC	8 cut(s)	419, 1194, 1712, 1861, 1975, 2094, 2141, 2350
PspPPI	RGGWCCY	4 cut(s)	1861, 1975, 2141, 2350
PspXI	VCTCGAGB	1 cut(s)	918
PstNI	CAGNNNCTG	1 cut(s)	2471
PsuI	RGATCY	8 cut(s)	265, 413, 900, 1456, 1594, 1918, 2449, 2561
PvuI	CGATCG	1 cut(s)	127
RsaI	GTAC	6 cut(s)	426, 756, 862, 959, 1465, 2203
RsaNI	GTAC	6 cut(s)	425, 755, 861, 958, 1464, 2202
RseI	CAYNNNNRTG	2 cut(s)	1398, 2652
SacI	GAGCTC	1 cut(s)	120
SaqAI	TTAA	8 cut(s)	522, 531, 599, 689, 1143, 1815, 2207, 2376
Sau96I	GGNCC	8 cut(s)	419, 1194, 1712, 1861, 1975, 2094, 2141, 2350
SchI	GAGTC	3 cut(s)	212, 394, 2054
ScrFI	CCNGG	6 cut(s)	88, 270, 298, 1299, 2097, 2460
SduI	GDGCHC	5 cut(s)	75, 120, 337, 1299, 1777
SfaNI	GCATC	9 cut(s)	119, 916, 1045, 2066, 2090, 2127, 2172, 2202, 2488
SfcI	CTRYAG	2 cut(s)	986, 1743
SfoI	GGCGCC	2 cut(s)	92, 1802
Sfr274I	CTCGAG	1 cut(s)	918
SfuI	TTCGAA	1 cut(s)	1157
SinI	GGWCC	6 cut(s)	419, 1712, 1861, 1975, 2141, 2350
SlaI	CTCGAG	1 cut(s)	918
SmiMI	CAYNNNNRTG	2 cut(s)	1398, 2652
SmlI	CTYRAG	5 cut(s)	246, 918, 1629, 1890, 2354
SmoI	CTYRAG	5 cut(s)	246, 918, 1629, 1890, 2354
SseBI	AGGCCT	3 cut(s)	86, 916, 1103
SsiI	CCGC	8 cut(s)	54, 97, 217, 826, 904, 1139, 2215, 2440
SspDI	GGCGCC	2 cut(s)	90, 1800
SspMI	CTAG	5 cut(s)	966, 1461, 2024, 2130, 2288
SstI	GAGCTC	1 cut(s)	120
StuI	AGGCCT	3 cut(s)	86, 916, 1103
StyD4I	CCNGG	6 cut(s)	86, 268, 296, 1297, 2095, 2458
StyI	CCWWGG	3 cut(s)	428, 561, 2565
TaaI	ACNGT	5 cut(s)	741, 797, 957, 1669, 2185
TaiI	ACGT	4 cut(s)	1075, 2162, 2207, 2714
TaqI	TCGA	5 cut(s)	127, 919, 1157, 1288, 1534
TatI	WGTACW	1 cut(s)	1463
TauI	GCSGC	3 cut(s)	57, 829, 2218
TfiI	GAWTC	4 cut(s)	1004, 1154, 1765, 2468
Tru1I	TTAA	8 cut(s)	522, 531, 599, 689, 1143, 1815, 2207, 2376
Tru9I	TTAA	8 cut(s)	522, 531, 599, 689, 1143, 1815, 2207, 2376
TscAI	CASTG	2 cut(s)	544, 2190
TseFI	GTSAC	2 cut(s)	1276, 2046
TseI	GCWGC	7 cut(s)	36, 1886, 2266, 2269, 2272, 2521, 2581
Tsp45I	GTSAC	2 cut(s)	1276, 2046
TspDTI	ATGAA	9 cut(s)	159, 161, 212, 341, 459, 617, 672, 1727, 1973
TspGWI	ACGGA	2 cut(s)	933, 2189
TspRI	CASTG	2 cut(s)	544, 2190
Van91I	CCANNNNNTGG	2 cut(s)	562, 1721
VpaK11BI	GGWCC	6 cut(s)	419, 1712, 1861, 1975, 2141, 2350
VspI	ATTAAT	2 cut(s)	522, 599
XagI	CCTNNNNNAGG	1 cut(s)	2406
XapI	RAATTY	2 cut(s)	649, 1009
XceI	RCATGY	2 cut(s)	294, 385
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2129
XhoI	CTCGAG	1 cut(s)	918
XmnI	GAANNNNTTC	1 cut(s)	1330
XspI	CTAG	5 cut(s)	966, 1461, 2024, 2130, 2288
ZraI	GACGTC	1 cut(s)	1073
Zsp2I	ATGCAT	2 cut(s)	668, 2111

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.