Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005133

Physical Location & Seq

Genomic Coordinates Reverse (-)

38918700 .. 38926108

Gene Structure 7409 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1302500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

ATGTCGTCGTCGTCGGCTTCCAAGGTCTGCTACAATCCTGAGTGCAAGGAGGTGAAATCGGAGTTTCCGAGAAAGGGCTGGCGGCTCCGGACCGGCGAGTACTCCGAGCTGTGCGATCGGTGCTATGTTGCTTATGAGGAAGGAAGATTCTGTGATACCTTTCATATGAATGCTGCTGGTTGGAGGTGCTGTGAGTCTTGTGGGAAGCGGGTTCATTGTGGATGCATCGTTTCAAGTCACGCATTTACATTGTTGGATCCCGGAGGAATTGAGTGCATGACATGTGCTAGGAAAAATTATGTTATGACATCAAACCCAGCATGGCCAACATCTATGTTCTTCCATTCTCCTTTATCTGAGAGACTTAAGGACCTGTCTACCAAAAATTGGACTCAGTTGGCTGGATCTGGTCCTGTACCATGGAAGCAAGCACCCAGTTTATTCAATTCTTCTATTCCTTCATCTGAGTTGCATGCAAGGGTGCCACATGAAGTTGATGGTTCAACTGGAATTAACAAACTTAATGCCAGCGAGAGAATATTTGTCTCCACCTTGGAAAAGAAGAAAGTTGAGGAATTTTCTGAAAGACTAATGAAAATAAACCTCACTATTGGCACACAACAAAGTCTTGAAAATGGGATTGGGAACCCTGGAAATAACATGGAGGAGAAAAGTAGTTCTTGTTTAAATATATCCCAGCAGTCTGCCTCTTTGAAGGAAGAACCGTCTACTCCACAGTTTGTACCTTATGGATCTGCAAGTGAAACAAACCCACAACTGGGGGTTACTGGAACTAATTTGTGGCCAATCGCTCCTTTGGCAAAGAATTATAATGGTAATTCACATAATGGAGTTGAATCATCTGGTGATACTCAGATCCGCAATGCAAGGCCTCGAGCAGATGCCCGGGGAAGAAATCAGTTACTTCCTCGGTACTGGCCTAGATTCACTGATCAAGAACTGCAGCATATATCTGGAGATAAAAATACTGTAATCACTCCACTATTCGAGAAAATGTTGAGTGCTAGTGATGCTGGACGGATTGGGCGTTTGGTGTTGCCAAAAAAATGTGCAGAGGCCTACTTTCCTCCAATTTCTCAACCCGAAGGGTTACCTCTTAAAGTTCAGGATTCAAAAGGCAAGGAATGGATTTTTCAGTTTCGCTTCTGGCCCAACAATAATAGCAGAATGTATGTTTTGGAGGGGGTTACCCCTTGCATACAATCCATGCAATTGCTGGCCGGTGACATAGTGACATTTAGTCGATTGGAACCAGAAGGGAAACTGGTTATGGGGTTCAGAAAGGCTTCAACTTCCCTACAATCTGATCAGGAAAATGAGGCAAACAAGACAACCAACGGAGTTTCAGCCCATGCAGAAGTTGAAGTGGCAGATCCTAGCTCATGGTCTAATGTTGATAAGTCAGGTTACATAGCAAAAGAAGCACTGGCAGATAAATATTATATGCCTAAAAAAAGAAAGAACAGTAACTTGGGTTCTAAGAGTAAGCGTCTGAGAATTGAAAATGAAGACTTGATAGAACTGAAGGTTACATGGGAAGAAGCTCAAGGCCTGCTTCGCCCTCCTGCTAACCATGTTCCCACTGTTGTGGTGATTGAAGGCTTTGAGTTTGAGGAATATGAGGATGCTCCACTTCTTGGGAAGCCAACAATTTATGCGACGGGCAGAGCAGGTGAAAGGATTCAGTGGGCTCAATGCGAAGATTGTTTCAAATGGCGCAAACTGCCTACCAATGCTCTTCTACCATCAAAGTGGACTTGTTCTGAGAATTCATGGGACCCTGAAAGATCTTTGTGTTCAGCAGCTCAAGAATTGACAACGGAACAGCTTCAAGATTTGCTACCCCGCTGTAACACAGTTGCTTCTAAGAAAATGAAGGCTGCTAAACAGGACCCCGATAACATTGAAGTACTGGAGGGGCTTGACACGCTCGCGAACCTAGCCATCTTGGGAGAGGGTGAGTCACTACCATCATCAGCTCAGGCCACCACAAAGCACCCCCGACATAGGCCTGGATGTTCATGTATTGTCTGCATCCAGCCCCCTAGTGGGAAGGGTCCTAAACACAAGCAAACATGCACCTGCAATGTGTGCTTAACAGTGAAGCGTCGCTTCCGTACATTAATGCTACGGCGCGAGAAGAAACAGTCAGATAAAGAAGCAGAAACTACACGGAAAAAGCAGCACAAGGGGCAATTACTGGTGGTTGATAAACCGGAGGATGACACCCTTTTATATTGTAACGCAGGAAGCAGTAGTCCAAACCAGAAAATAGAGATCCTTGAACTTTCCGATGATGACCTTAACCTGACGAAATCCTCAACTTCACCTTTTAAAGGCCAAATTGACCTGAATATCCAGCCGGAGCGGGAGGATGAACTGTCGCCTGGATCCGATTCAGGCGGCATGATGAAGTTGCTTCACGATGCCACAGCAAGGTATCTCAGGCAGCAGAGATTGTCAAGCTCTGGTGGTGGTGAAAAGTCATCAGGTAACGGGTCACCACGAGCTGCAAGTGAAGCAGGAGGGGGAGAACCCCTCAGCAACGGTGTAAACCTCGAAAGTAGCAATCATGATGGTGGCAACGACTGTCCTGCAACATTATCCATCACAGCATCAGCATCAATGACTGCTACGGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B3 zf-CW

Protein Analysis

888

Amino Acids

97.78

Weight (kDa)

7.42

Isoelectric Point (pI)

48.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf_VAL1_N	PF25813	53 - 100	1.2e-22	VAL1-like, N-terminal zinc finger domain
B3	PF02362	336 - 435	1.7e-15	B3 DNA binding domain
zf-CW	PF07496	569 - 611	1.3e-11	CW-type Zinc Finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21550 AT4G21550 AT4G21550
fragaria_vesca	FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120 FvH4_3g04120
malus_domestica	MD05G1324700.v1.1 MD10G1302500.v1.1
prunus_persica	Prupe.4G036100_v2.0.a1
pyrus_communis	pycom05g29890 pycom10g25500
rosa_chinensis	RchiOBHm_Chr5g0006571
rosa_laevigata	RLG00000031400
rosa_multiflora	Rmu_sc0007807.1_g000001 Rmu_sc0011154.1_g000008 Rmu_sc0011154.1_g000009
rosa_roxburghii	Rroxscaffold_1G00069470
rosa_rugosa	Rorug04G0422600.1 Rorug04G0422700
rosa_samantha	Rh5AG049800 Rh5BG049100 Rh5CG057400 Rh5DG048500
rosa_wichuraiana	Rw5G004990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	831
AarI	CACCTGC	2 cut(s)	1682, 2111
Acc36I	ACCTGC	2 cut(s)	1682, 2111
AccB1I	GGYRCC	1 cut(s)	481
AccB7I	CCANNNNNTGG	2 cut(s)	387, 1658
AccBSI	CCGCTC	1 cut(s)	2389
AccI	GTMKAC	2 cut(s)	377, 728
AccII	CGCG	2 cut(s)	1955, 2157
AccIII	TCCGGA	1 cut(s)	87
AciI	CCGC	6 cut(s)	82, 208, 880, 1867, 2389, 2424
AclWI	GGATC	9 cut(s)	251, 264, 412, 760, 871, 1388, 2293, 2406, 2419
AcoI	YGGCCR	3 cut(s)	323, 803, 1239
AcsI	RAATTY	2 cut(s)	575, 1789
AcuI	CTGAAG	1 cut(s)	1565
AfaI	GTAC	6 cut(s)	101, 417, 744, 935, 1932, 2140
AflII	CTTAAG	1 cut(s)	365
AflIII	ACRYGT	1 cut(s)	281
AhdI	GACNNNNNGTC	1 cut(s)	1260
AjnI	CCWGG	3 cut(s)	649, 2032, 2407
AjuI	GAANNNNNNNTTGG	4 cut(s)	624, 656, 1475, 1507
AleI	CACNNNNGTG	1 cut(s)	1607
AluBI	AGCT	8 cut(s)	109, 1401, 1565, 1826, 1849, 2000, 2487, 2531
AluI	AGCT	8 cut(s)	109, 1401, 1565, 1826, 1849, 2000, 2487, 2531
Alw26I	GTCTC	2 cut(s)	355, 550
AlwI	GGATC	9 cut(s)	251, 264, 412, 760, 871, 1388, 2293, 2406, 2419
Ama87I	CYCGRG	2 cut(s)	894, 906
Aor13HI	TCCGGA	1 cut(s)	87
ApeKI	GCWGC	7 cut(s)	173, 964, 1823, 1901, 2203, 2470, 2531
ApoI	RAATTY	2 cut(s)	575, 1789
AseI	ATTAAT	1 cut(s)	2144
Asp700I	GAANNNNTTC	3 cut(s)	1306, 1701, 1848
AspLEI	GCGC	2 cut(s)	1740, 2157
AspS9I	GGNCC	7 cut(s)	90, 370, 410, 1170, 1798, 1912, 2078
AsuC2I	CCSGG	3 cut(s)	261, 907, 908
AsuHPI	GGTGA	9 cut(s)	64, 878, 1256, 1624, 1706, 1991, 2340, 2510, 2514
AvaI	CYCGRG	2 cut(s)	894, 906
AvaII	GGWCC	6 cut(s)	90, 370, 410, 1798, 1912, 2078
BalI	TGGCCA	2 cut(s)	325, 805
BamHI	GGATCC	2 cut(s)	256, 2411
BanI	GGYRCC	1 cut(s)	481
BanII	GRGCYC	1 cut(s)	1714
BauI	CACGAG	1 cut(s)	2526
BbsI	GAAGAC	1 cut(s)	1536
BbvCI	CCTCAGC	1 cut(s)	2561
BbvI	GCAGC	7 cut(s)	160, 976, 1835, 1888, 2215, 2482, 2518
BccI	CCATC	6 cut(s)	491, 1774, 1973, 1999, 2591, 2636
BceAI	ACGGC	1 cut(s)	2168
BcgI	CGANNNNNNTGC	2 cut(s)	2597, 2631
BciT130I	CCWGG	3 cut(s)	651, 2034, 2409
BclI	TGATCA	2 cut(s)	952, 1327
BcnI	CCSGG	3 cut(s)	261, 907, 908
BcoDI	GTCTC	2 cut(s)	355, 550
BfaI	CTAG	6 cut(s)	288, 942, 1026, 1398, 1961, 2067
BfmI	CTRYAG	1 cut(s)	962
BfrI	CTTAAG	1 cut(s)	365
BfuAI	ACCTGC	2 cut(s)	1682, 2111
BglII	AGATCT	1 cut(s)	1808
BisI	GCNGC	9 cut(s)	83, 174, 965, 1824, 1902, 2204, 2425, 2471, 2532
BlsI	GCNGC	9 cut(s)	84, 175, 966, 1825, 1903, 2205, 2426, 2472, 2533
BmcAI	AGTACT	2 cut(s)	101, 1932
Bme1390I	CCNGG	6 cut(s)	261, 651, 907, 908, 2034, 2409
Bme18I	GGWCC	6 cut(s)	90, 370, 410, 1798, 1912, 2078
BmeRI	GACNNNNNGTC	1 cut(s)	1260
BmeT110I	CYCGRG	2 cut(s)	894, 906
BmgT120I	GGNCC	7 cut(s)	90, 370, 410, 1170, 1798, 1912, 2078
BmrFI	CCNGG	6 cut(s)	261, 651, 907, 908, 2034, 2409
BmrI	ACTGGG	2 cut(s)	429, 788
BmsI	GCATC	9 cut(s)	212, 234, 892, 1021, 1636, 2064, 2437, 2645, 2651
BmuI	ACTGGG	2 cut(s)	429, 788
BpiI	GAAGAC	1 cut(s)	1536
BplI	GAGNNNNNCTC	2 cut(s)	2544, 2576
BpmI	CTGGAG	2 cut(s)	996, 1955
Bpu10I	CCTNAGC	2 cut(s)	2001, 2561
BpuEI	CTTGAG	2 cut(s)	1551, 1812
BpuMI	CCSGG	3 cut(s)	261, 907, 908
BsaJI	CCNNGG	7 cut(s)	21, 419, 552, 649, 906, 907, 929
BsaWI	WCCGGW	2 cut(s)	87, 2236
BsaXI	ACNNNNNCTCC	2 cut(s)	175, 205
Bse118I	RCCGGY	2 cut(s)	92, 1241
Bse1I	ACTGG	9 cut(s)	435, 511, 783, 793, 941, 1290, 1452, 1938, 2226
Bse3DI	GCAATG	2 cut(s)	889, 2113
BseAI	TCCGGA	1 cut(s)	87
BseBI	CCWGG	3 cut(s)	651, 2034, 2409
BseDI	CCNNGG	7 cut(s)	21, 419, 552, 649, 906, 907, 929
BseGI	GGATG	6 cut(s)	227, 1651, 2042, 2055, 2248, 2401
BseMI	GCAATG	2 cut(s)	889, 2113
BseNI	ACTGG	9 cut(s)	435, 511, 783, 793, 941, 1290, 1452, 1938, 2226
BseRI	GAGGAG	1 cut(s)	680
BseXI	GCAGC	7 cut(s)	160, 976, 1835, 1888, 2215, 2482, 2518
BseYI	CCCAGC	2 cut(s)	316, 696
BsgI	GTGCAG	1 cut(s)	1092
Bsh1236I	CGCG	2 cut(s)	1955, 2157
Bsh1285I	CGRYCG	1 cut(s)	118
BshNI	GGYRCC	1 cut(s)	481
BsiEI	CGRYCG	1 cut(s)	118
BsiHKCI	CYCGRG	2 cut(s)	894, 906
BsiSI	CCGG	7 cut(s)	88, 93, 261, 907, 1242, 2237, 2384
BslFI	GGGAC	1 cut(s)	1811
BsmAI	GTCTC	2 cut(s)	355, 550
BsmFI	GGGAC	1 cut(s)	1811
BsmI	GAATGC	1 cut(s)	175
BsoBI	CYCGRG	2 cut(s)	894, 906
Bsp1286I	GDGCHC	1 cut(s)	1714
Bsp13I	TCCGGA	1 cut(s)	87
Bsp19I	CCATGG	1 cut(s)	419
Bsp68I	TCGCGA	1 cut(s)	1955
BspACI	CCGC	6 cut(s)	82, 208, 880, 1867, 2389, 2424
BspEI	TCCGGA	1 cut(s)	87
BspFNI	CGCG	2 cut(s)	1955, 2157
BspHI	TCATGA	1 cut(s)	2593
BspMAI	CTGCAG	1 cut(s)	966
BspMI	ACCTGC	2 cut(s)	1682, 2111
BspPI	GGATC	9 cut(s)	251, 264, 412, 760, 871, 1388, 2293, 2406, 2419
BspQI	GCTCTTC	1 cut(s)	1764
BspT107I	GGYRCC	1 cut(s)	481
BspTI	CTTAAG	1 cut(s)	365
BsrBI	CCGCTC	1 cut(s)	2389
BsrDI	GCAATG	2 cut(s)	889, 2113
BsrFI	RCCGGY	2 cut(s)	92, 1241
BsrI	ACTGG	9 cut(s)	435, 511, 783, 793, 941, 1290, 1452, 1938, 2226
BssAI	RCCGGY	2 cut(s)	92, 1241
BssECI	CCNNGG	7 cut(s)	21, 419, 552, 649, 906, 907, 929
BssSI	CACGAG	1 cut(s)	2526
BssT1I	CCWWGG	3 cut(s)	21, 419, 552
Bst2BI	CACGAG	1 cut(s)	2526
Bst2UI	CCWGG	3 cut(s)	651, 2034, 2409
Bst6I	CTCTTC	1 cut(s)	1764
BstAFI	CTTAAG	1 cut(s)	365
BstAPI	GCANNNNNTGC	1 cut(s)	2112
BstC8I	GCNNGC	7 cut(s)	80, 429, 474, 529, 1239, 1574, 1953
BstDSI	CCRYGG	1 cut(s)	419
BstEII	GGTNACC	3 cut(s)	1110, 1207, 2520
BstENI	CCTNNNNNAGG	1 cut(s)	2355
BstF5I	GGATG	6 cut(s)	227, 1651, 2042, 2055, 2248, 2401
BstFNI	CGCG	2 cut(s)	1955, 2157
BstHHI	GCGC	2 cut(s)	1740, 2157
BstMAI	GTCTC	2 cut(s)	355, 550
BstMCI	CGRYCG	1 cut(s)	118
BstNI	CCWGG	3 cut(s)	651, 2034, 2409
BstNSI	RCATGY	3 cut(s)	285, 476, 2100
BstPI	GGTNACC	3 cut(s)	1110, 1207, 2520
BstSCI	CCNGG	6 cut(s)	259, 649, 905, 906, 2032, 2407
BstSFI	CTRYAG	1 cut(s)	962
BstUI	CGCG	2 cut(s)	1955, 2157
BstV1I	GCAGC	7 cut(s)	160, 976, 1835, 1888, 2215, 2482, 2518
BstV2I	GAAGAC	1 cut(s)	1536
BstX2I	RGATCY	8 cut(s)	256, 404, 752, 876, 1393, 1808, 2298, 2411
BstXI	CCANNNNNNTGG	2 cut(s)	1609, 1773
BstYI	RGATCY	8 cut(s)	256, 404, 752, 876, 1393, 1808, 2298, 2411
BtgI	CCRYGG	1 cut(s)	419
BtsCI	GGATG	6 cut(s)	227, 1651, 2042, 2055, 2248, 2401
BtsIMutI	CAGTG	5 cut(s)	948, 1445, 1602, 1712, 2127
BtuMI	TCGCGA	1 cut(s)	1955
BveI	ACCTGC	2 cut(s)	1682, 2111
Cac8I	GCNNGC	7 cut(s)	80, 429, 474, 529, 1239, 1574, 1953
CciI	TCATGA	1 cut(s)	2593
CfoI	GCGC	2 cut(s)	1740, 2157
Cfr10I	RCCGGY	2 cut(s)	92, 1241
Cfr13I	GGNCC	7 cut(s)	90, 370, 410, 1170, 1798, 1912, 2078
Cfr9I	CCCGGG	1 cut(s)	906
CpoI	CGGWCCG	1 cut(s)	90
CseI	GACGC	2 cut(s)	1499, 2117
Csp6I	GTAC	6 cut(s)	100, 416, 743, 934, 1931, 2139
CspI	CGGWCCG	1 cut(s)	90
CviQI	GTAC	6 cut(s)	100, 416, 743, 934, 1931, 2139
DraI	TTTAAA	2 cut(s)	687, 2356
DriI	GACNNNNNGTC	1 cut(s)	1260
EaeI	YGGCCR	3 cut(s)	323, 803, 1239
Eam1104I	CTCTTC	1 cut(s)	1764
Eam1105I	GACNNNNNGTC	1 cut(s)	1260
EarI	CTCTTC	1 cut(s)	1764
Eco130I	CCWWGG	3 cut(s)	21, 419, 552
Eco147I	AGGCCT	4 cut(s)	892, 1079, 1572, 2032
Eco24I	GRGCYC	1 cut(s)	1714
Eco47I	GGWCC	6 cut(s)	90, 370, 410, 1798, 1912, 2078
Eco57I	CTGAAG	1 cut(s)	1565
Eco88I	CYCGRG	2 cut(s)	894, 906
Eco91I	GGTNACC	3 cut(s)	1110, 1207, 2520
EcoNI	CCTNNNNNAGG	1 cut(s)	2355
EcoO109I	RGGNCCY	4 cut(s)	370, 1798, 1912, 2078
EcoO65I	GGTNACC	3 cut(s)	1110, 1207, 2520
EcoRI	GAATTC	1 cut(s)	1789
EcoRII	CCWGG	3 cut(s)	649, 2032, 2407
EcoT14I	CCWWGG	3 cut(s)	21, 419, 552
EcoT22I	ATGCAT	1 cut(s)	227
EcoT38I	GRGCYC	1 cut(s)	1714
ErhI	CCWWGG	3 cut(s)	21, 419, 552
FalI	AAGNNNNNCTT	6 cut(s)	664, 696, 1560, 1592, 2117, 2149
FaqI	GGGAC	1 cut(s)	1811
FauI	CCCGC	3 cut(s)	201, 1874, 2382
FauNDI	CATATG	1 cut(s)	165
FbaI	TGATCA	2 cut(s)	952, 1327
FblI	GTMKAC	2 cut(s)	377, 728
Fnu4HI	GCNGC	9 cut(s)	83, 174, 965, 1824, 1902, 2204, 2425, 2471, 2532
FokI	GGATG	6 cut(s)	234, 1658, 2042, 2049, 2255, 2408
FriOI	GRGCYC	1 cut(s)	1714
Fsp4HI	GCNGC	9 cut(s)	83, 174, 965, 1824, 1902, 2204, 2425, 2471, 2532
FspBI	CTAG	6 cut(s)	288, 942, 1026, 1398, 1961, 2067
GlaI	GCGC	2 cut(s)	1739, 2156
GluI	GCNGC	9 cut(s)	83, 174, 965, 1824, 1902, 2204, 2425, 2471, 2532
GsaI	CCCAGC	2 cut(s)	320, 700
GsuI	CTGGAG	2 cut(s)	996, 1955
HapII	CCGG	7 cut(s)	88, 93, 261, 907, 1242, 2237, 2384
HgaI	GACGC	2 cut(s)	1499, 2117
HhaI	GCGC	2 cut(s)	1740, 2157
Hin6I	GCGC	2 cut(s)	1738, 2155
HinP1I	GCGC	2 cut(s)	1738, 2155
HinfI	GANTC	9 cut(s)	147, 194, 391, 857, 945, 1130, 1702, 1982, 2417
HpaII	CCGG	7 cut(s)	88, 93, 261, 907, 1242, 2237, 2384
HphI	GGTGA	9 cut(s)	64, 878, 1256, 1624, 1706, 1991, 2340, 2510, 2514
Hpy166II	GTNNAC	4 cut(s)	378, 729, 1776, 2575
Hpy8I	GTNNAC	4 cut(s)	378, 729, 1776, 2575
Hpy99I	CGWCG	5 cut(s)	10, 13, 16, 1684, 2133
HpyAV	CCTTC	9 cut(s)	134, 468, 709, 1100, 1271, 1540, 1613, 1891, 2068
HspAI	GCGC	2 cut(s)	1738, 2155
KflI	GGGWCCC	1 cut(s)	1798
Kpn2I	TCCGGA	1 cut(s)	87
Ksp22I	TGATCA	2 cut(s)	952, 1327
LguI	GCTCTTC	1 cut(s)	1764
LmnI	GCTCC	4 cut(s)	90, 817, 1654, 2386
Lsp1109I	GCAGC	7 cut(s)	160, 976, 1835, 1888, 2215, 2482, 2518
LweI	GCATC	9 cut(s)	212, 234, 892, 1021, 1636, 2064, 2437, 2645, 2651
MaeI	CTAG	6 cut(s)	288, 942, 1026, 1398, 1961, 2067
MbiI	CCGCTC	1 cut(s)	2389
MfeI	CAATTG	1 cut(s)	1232
MflI	RGATCY	8 cut(s)	256, 404, 752, 876, 1393, 1808, 2298, 2411
MhlI	GDGCHC	1 cut(s)	1714
MlsI	TGGCCA	2 cut(s)	325, 805
MluNI	TGGCCA	2 cut(s)	325, 805
MlyI	GAGTC	3 cut(s)	203, 385, 1991
MmeI	TCCRAC	2 cut(s)	161, 234
Mox20I	TGGCCA	2 cut(s)	325, 805
Mph1103I	ATGCAT	1 cut(s)	227
MroI	TCCGGA	1 cut(s)	87
MroXI	GAANNNNTTC	3 cut(s)	1306, 1701, 1848
MscI	TGGCCA	2 cut(s)	325, 805
MseI	TTAA	9 cut(s)	366, 513, 522, 686, 1119, 2117, 2144, 2325, 2355
MslI	CAYNNNNRTG	4 cut(s)	168, 1607, 1771, 2598
Msp20I	TGGCCA	2 cut(s)	325, 805
MspA1I	CMGCKG	1 cut(s)	1869
MspCI	CTTAAG	1 cut(s)	365
MspI	CCGG	7 cut(s)	88, 93, 261, 907, 1242, 2237, 2384
MspR9I	CCNGG	6 cut(s)	261, 651, 907, 908, 2034, 2409
MunI	CAATTG	1 cut(s)	1232
Mva1269I	GAATGC	1 cut(s)	175
MvaI	CCWGG	3 cut(s)	651, 2034, 2409
MvnI	CGCG	2 cut(s)	1955, 2157
NciI	CCSGG	3 cut(s)	261, 907, 908
NcoI	CCATGG	1 cut(s)	419
NdeI	CATATG	1 cut(s)	165
NmuCI	GTSAC	5 cut(s)	236, 1244, 1252, 1983, 2520
NruI	TCGCGA	1 cut(s)	1955
NsiI	ATGCAT	1 cut(s)	227
NspI	RCATGY	3 cut(s)	285, 476, 2100
OliI	CACNNNNGTG	1 cut(s)	1607
PaeI	GCATGC	1 cut(s)	476
PaeR7I	CTCGAG	1 cut(s)	894
PagI	TCATGA	1 cut(s)	2593
PaqCI	CACCTGC	2 cut(s)	1682, 2111
PceI	AGGCCT	4 cut(s)	892, 1079, 1572, 2032
PciI	ACATGT	1 cut(s)	281
PciSI	GCTCTTC	1 cut(s)	1764
PcsI	WCGNNNNNNNCGW	2 cut(s)	65, 1045
PctI	GAATGC	1 cut(s)	175
PdmI	GAANNNNTTC	3 cut(s)	1306, 1701, 1848
PfeI	GAWTC	6 cut(s)	147, 857, 945, 1130, 1702, 2417
PflMI	CCANNNNNTGG	2 cut(s)	387, 1658
PfoI	TCCNGGA	1 cut(s)	259
PkrI	GCNGC	9 cut(s)	84, 175, 966, 1825, 1903, 2205, 2426, 2472, 2533
Ple19I	CGATCG	1 cut(s)	118
PleI	GAGTC	3 cut(s)	202, 385, 1990
PpsI	GAGTC	3 cut(s)	202, 385, 1990
PpuMI	RGGWCCY	4 cut(s)	370, 1798, 1912, 2078
PscI	ACATGT	1 cut(s)	281
PshBI	ATTAAT	1 cut(s)	2144
PsiI	TTATAA	1 cut(s)	831
Psp5II	RGGWCCY	4 cut(s)	370, 1798, 1912, 2078
Psp6I	CCWGG	3 cut(s)	649, 2032, 2407
PspEI	GGTNACC	3 cut(s)	1110, 1207, 2520
PspFI	CCCAGC	2 cut(s)	316, 696
PspGI	CCWGG	3 cut(s)	649, 2032, 2407
PspPI	GGNCC	7 cut(s)	90, 370, 410, 1170, 1798, 1912, 2078
PspPPI	RGGWCCY	4 cut(s)	370, 1798, 1912, 2078
PspXI	VCTCGAGB	1 cut(s)	894
PsrI	GAACNNNNNNTAC	2 cut(s)	1480, 1512
PstI	CTGCAG	1 cut(s)	966
PsuI	RGATCY	8 cut(s)	256, 404, 752, 876, 1393, 1808, 2298, 2411
PvuI	CGATCG	1 cut(s)	118
RruI	TCGCGA	1 cut(s)	1955
RsaI	GTAC	6 cut(s)	101, 417, 744, 935, 1932, 2140
RsaNI	GTAC	6 cut(s)	100, 416, 743, 934, 1931, 2139
RseI	CAYNNNNRTG	4 cut(s)	168, 1607, 1771, 2598
Rsr2I	CGGWCCG	1 cut(s)	90
RsrII	CGGWCCG	1 cut(s)	90
SapI	GCTCTTC	1 cut(s)	1764
SaqAI	TTAA	9 cut(s)	366, 513, 522, 686, 1119, 2117, 2144, 2325, 2355
SatI	GCNGC	9 cut(s)	83, 174, 965, 1824, 1902, 2204, 2425, 2471, 2532
Sau96I	GGNCC	7 cut(s)	90, 370, 410, 1170, 1798, 1912, 2078
ScaI	AGTACT	2 cut(s)	101, 1932
SchI	GAGTC	3 cut(s)	203, 385, 1991
ScrFI	CCNGG	6 cut(s)	261, 651, 907, 908, 2034, 2409
SduI	GDGCHC	1 cut(s)	1714
SfaNI	GCATC	9 cut(s)	212, 234, 892, 1021, 1636, 2064, 2437, 2645, 2651
SfcI	CTRYAG	1 cut(s)	962
Sfr274I	CTCGAG	1 cut(s)	894
SinI	GGWCC	6 cut(s)	90, 370, 410, 1798, 1912, 2078
SlaI	CTCGAG	1 cut(s)	894
SmaI	CCCGGG	1 cut(s)	908
SmiMI	CAYNNNNRTG	4 cut(s)	168, 1607, 1771, 2598
SmlI	CTYRAG	4 cut(s)	365, 894, 1566, 1827
SmoI	CTYRAG	4 cut(s)	365, 894, 1566, 1827
SphI	GCATGC	1 cut(s)	476
SseBI	AGGCCT	4 cut(s)	892, 1079, 1572, 2032
SsiI	CCGC	6 cut(s)	82, 208, 880, 1867, 2389, 2424
SspI	AATATT	2 cut(s)	540, 1460
SspMI	CTAG	6 cut(s)	288, 942, 1026, 1398, 1961, 2067
StuI	AGGCCT	4 cut(s)	892, 1079, 1572, 2032
StyD4I	CCNGG	6 cut(s)	259, 649, 905, 906, 2032, 2407
StyI	CCWWGG	3 cut(s)	21, 419, 552
TaqI	TCGA	4 cut(s)	895, 1008, 1264, 2580
TatI	WGTACW	2 cut(s)	99, 1930
TauI	GCSGC	2 cut(s)	85, 2427
TfiI	GAWTC	6 cut(s)	147, 857, 945, 1130, 1702, 2417
Tru1I	TTAA	9 cut(s)	366, 513, 522, 686, 1119, 2117, 2144, 2325, 2355
Tru9I	TTAA	9 cut(s)	366, 513, 522, 686, 1119, 2117, 2144, 2325, 2355
TscAI	CASTG	5 cut(s)	955, 1452, 1609, 1712, 2127
TseFI	GTSAC	5 cut(s)	236, 1244, 1252, 1983, 2520
TseI	GCWGC	7 cut(s)	173, 964, 1823, 1901, 2203, 2470, 2531
Tsp45I	GTSAC	5 cut(s)	236, 1244, 1252, 1983, 2520
TspGWI	ACGGA	5 cut(s)	1054, 1374, 1856, 2126, 2209
TspMI	CCCGGG	1 cut(s)	906
TspRI	CASTG	5 cut(s)	955, 1452, 1609, 1712, 2127
Van91I	CCANNNNNTGG	2 cut(s)	387, 1658
Vha464I	CTTAAG	1 cut(s)	365
VpaK11BI	GGWCC	6 cut(s)	90, 370, 410, 1798, 1912, 2078
VspI	ATTAAT	1 cut(s)	2144
XagI	CCTNNNNNAGG	1 cut(s)	2355
XapI	RAATTY	2 cut(s)	575, 1789
XceI	RCATGY	3 cut(s)	285, 476, 2100
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1234, 2066
XhoI	CTCGAG	1 cut(s)	894
XmaI	CCCGGG	1 cut(s)	906
XmiI	GTMKAC	2 cut(s)	377, 728
XmnI	GAANNNNTTC	3 cut(s)	1306, 1701, 1848
XspI	CTAG	6 cut(s)	288, 942, 1026, 1398, 1961, 2067
ZrmI	AGTACT	2 cut(s)	101, 1932
Zsp2I	ATGCAT	1 cut(s)	227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.