MD05G1358000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013287
Physical Location & Seq
Reverse (-)
47343555 .. 47345625
2071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1358000.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGTGCTGGTTCTTAACCTAATCCCGCCATCCCAAGCGCAAACCCTACTCCTTCAGTCGTTGTCGTCGTCCTCCTCCTCCACTCGCCCACTGCCGACTGTCATTTCCCGCCCAAATGAAGCAACCCAGGACTGGAATTCTCTGCTGTTCAAGCTCAAGTGCCGCGGCAGGTTCTCTTGCCTTTTCTCAGACAATCGCAAACAGGACCAAGCTAGGAAAGCATTAGAAGGTGCACTTGGTGGAAAGAAAGATGAATTCAAGAAATGGGACAAAGAAATTAAGAGAAGAGAGGAGGTGGGTGGAGGAGGTAGCGCTGGTGGTGGGGGATGGTTTGGGTGGGGTAGATGGTTTGGATGGTCGAATGATGGCAATTTCTGGCAAGAAGCACAACAAGCAAGTCTTGCTGTGATGGGTATCATTGTGATGTATCTCATAATAGCAAAGGGAGAACTGATGCTTGCTGTCATCTTCAATCCGTTGCTATATGCCTTGCGAGGGACTAGAAATGGGTTTGCTTTAATAACGTCTAGAATCTTTAGGAAGACAGCACCAGATGGCCATAATATCTCGATGAAGGAAGCCTACGGCACTGTCTCCGCTAAAGAGAGTGTTCTGAGAAAGTGGGGAAGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.39

Weight (kDa)

9.91

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013287)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20130 AT5G20130
fragaria_vesca FvH4_3g00960
malus_domestica MD05G1358000.v1.1 MD10G1331900.v1.1
prunus_persica Prupe.4G010200_v2.0.a1
pyrus_communis pycom05g32380
rosa_chinensis RchiOBHm_Chr5g0001481
rosa_laevigata RLG00000030932
rosa_multiflora Rmu_co8090336.1_g000001 Rmu_sc0003749.1_g000010
rosa_roxburghii Rroxscaffold_1G00074910
rosa_rugosa Rorug04G0390500
rosa_samantha Rh5AG011500 Rh5BG014000 Rh5CG012500 Rh5DG011700
rosa_wichuraiana Rw5G001090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 159
AccII CGCG 1 cut(s) 165
AciI CCGC 5 cut(s) 26, 109, 163, 165, 597
AcoI YGGCCR 1 cut(s) 556
AcsI RAATTY 2 cut(s) 136, 254
AcuI CTGAAG 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 239
AfeI AGCGCT 1 cut(s) 313
AfiI CCNNNNNNNGG 2 cut(s) 132, 495
AgsI TTSAA 3 cut(s) 151, 259, 472
AjnI CCWGG 1 cut(s) 126
AjuI GAANNNNNNNTTGG 2 cut(s) 219, 251
AluBI AGCT 2 cut(s) 154, 212
AluI AGCT 2 cut(s) 154, 212
Alw21I GWGCWC 1 cut(s) 235
Alw26I GTCTC 1 cut(s) 598
Alw44I GTGCAC 1 cut(s) 231
Aor51HI AGCGCT 1 cut(s) 313
AoxI GGCC 1 cut(s) 556
ApaLI GTGCAC 1 cut(s) 231
ApoI RAATTY 2 cut(s) 136, 254
AspLEI GCGC 2 cut(s) 40, 314
AspS9I GGNCC 1 cut(s) 205
AvaII GGWCC 1 cut(s) 205
BaeGI GKGCMC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 558
BarI GAAGNNNNNNTAC 2 cut(s) 566, 598
BbsI GAAGAC 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 235
BccI CCATC 7 cut(s) 37, 321, 339, 348, 359, 403, 548
BceAI ACGGC 1 cut(s) 601
BciT130I CCWGG 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 598
BfaI CTAG 4 cut(s) 213, 501, 528, 634
BfoI RGCGCY 1 cut(s) 315
BfuAI ACCTGC 1 cut(s) 159
BisI GCNGC 2 cut(s) 163, 166
BlsI GCNGC 2 cut(s) 164, 167
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 1 cut(s) 444
BpiI GAAGAC 1 cut(s) 548
BpuEI CTTGAG 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 126, 163
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 495
Bse1I ACTGG 1 cut(s) 137
Bse8I GATNNNNATC 1 cut(s) 413
BseBI CCWGG 1 cut(s) 128
BseDI CCNNGG 2 cut(s) 126, 163
BseGI GGATG 3 cut(s) 29, 332, 359
BseJI GATNNNNATC 1 cut(s) 413
BseLI CCNNNNNNNGG 2 cut(s) 132, 495
BseMII CTCAG 2 cut(s) 201, 605
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 4 cut(s) 64, 67, 305, 318
BseSI GKGCMC 1 cut(s) 235
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 1 cut(s) 558
BsiHKAI GWGCWC 1 cut(s) 235
BslFI GGGAC 2 cut(s) 281, 511
BslI CCNNNNNNNGG 2 cut(s) 132, 495
BsmAI GTCTC 1 cut(s) 598
BsmFI GGGAC 2 cut(s) 281, 511
BsnI GGCC 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 235
BspACI CCGC 5 cut(s) 26, 109, 163, 165, 597
BspANI GGCC 1 cut(s) 558
BspCNI CTCAG 2 cut(s) 200, 606
BspFNI CGCG 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 159
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 126, 163
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 2 cut(s) 100, 592
Bst6I CTCTTC 1 cut(s) 280
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 2 cut(s) 187, 614
BstDSI CCRYGG 1 cut(s) 163
BstF5I GGATG 3 cut(s) 29, 332, 359
BstFNI CGCG 1 cut(s) 165
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 2 cut(s) 40, 314
BstMAI GTCTC 1 cut(s) 598
BstMWI GCNNNNNNNGC 4 cut(s) 151, 218, 392, 401
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 126
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 1 cut(s) 165
BstV2I GAAGAC 1 cut(s) 548
BsuRI GGCC 1 cut(s) 558
BtgI CCRYGG 1 cut(s) 163
BtsCI GGATG 3 cut(s) 29, 332, 359
BtsI GCAGTG 1 cut(s) 89
BtsIMutI CAGTG 2 cut(s) 89, 588
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 1 cut(s) 459
CfoI GCGC 2 cut(s) 40, 314
Cfr13I GGNCC 1 cut(s) 205
Cfr42I CCGCGG 1 cut(s) 166
CviJI RGCY 4 cut(s) 154, 212, 558, 581
CviKI_1 RGCY 4 cut(s) 154, 212, 558, 581
DdeI CTNAG 2 cut(s) 187, 614
DraIII CACNNNGTG 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 556
Eam1104I CTCTTC 1 cut(s) 280
EarI CTCTTC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 205
Eco47III AGCGCT 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 38
EcoRI GAATTC 2 cut(s) 136, 254
EcoRII CCWGG 1 cut(s) 126
FaiI YATR 4 cut(s) 434, 484, 486, 561
FalI AAGNNNNNCTT 4 cut(s) 219, 251, 384, 416
FaqI GGGAC 2 cut(s) 281, 511
FauI CCCGC 2 cut(s) 33, 116
Fnu4HI GCNGC 2 cut(s) 163, 166
FokI GGATG 3 cut(s) 16, 339, 366
Fsp4HI GCNGC 2 cut(s) 163, 166
FspBI CTAG 4 cut(s) 213, 501, 528, 634
GlaI GCGC 2 cut(s) 39, 313
GluI GCNGC 2 cut(s) 163, 166
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 1 cut(s) 558
HhaI GCGC 2 cut(s) 40, 314
Hin6I GCGC 2 cut(s) 38, 312
HinP1I GCGC 2 cut(s) 38, 312
HinfI GANTC 1 cut(s) 531
Hpy166II GTNNAC 1 cut(s) 233
Hpy188I TCNGA 2 cut(s) 190, 615
Hpy188III TCNNGA 3 cut(s) 259, 528, 568
Hpy8I GTNNAC 1 cut(s) 233
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 3 cut(s) 62, 221, 568
HpyCH4III ACNGT 2 cut(s) 100, 592
HpyCH4IV ACGT 1 cut(s) 524
HpyCH4V TGCA 1 cut(s) 233
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 218, 392, 401
HpyF3I CTNAG 2 cut(s) 187, 614
HpySE526I ACGT 1 cut(s) 524
HspAI GCGC 2 cut(s) 38, 312
KspI CCGCGG 1 cut(s) 166
LpnPI CCDG 8 cut(s) 113, 118, 140, 154, 188, 300, 361, 564
LweI GCATC 1 cut(s) 444
MaeI CTAG 4 cut(s) 213, 501, 528, 634
MaeII ACGT 1 cut(s) 524
MaeIII GTNAC 1 cut(s) 629
MboII GAAGA 3 cut(s) 297, 460, 553
MhlI GDGCHC 1 cut(s) 235
MlsI TGGCCA 1 cut(s) 558
MluCI AATT 4 cut(s) 136, 254, 276, 370
MluNI TGGCCA 1 cut(s) 558
MnlI CCTC 8 cut(s) 82, 85, 88, 283, 286, 296, 299, 488
Mox20I TGGCCA 1 cut(s) 558
MscI TGGCCA 1 cut(s) 558
MseI TTAA 3 cut(s) 15, 279, 518
MslI CAYNNNNRTG 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 4 cut(s) 151, 218, 392, 401
NmuCI GTSAC 1 cut(s) 629
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PfeI GAWTC 1 cut(s) 531
PkrI GCNGC 2 cut(s) 164, 167
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspPI GGNCC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 422
SacII CCGCGG 1 cut(s) 166
SaqAI TTAA 3 cut(s) 15, 279, 518
SatI GCNGC 2 cut(s) 163, 166
Sau96I GGNCC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 1 cut(s) 235
SetI ASST 8 cut(s) 21, 156, 173, 214, 232, 297, 310, 527
SfaNI GCATC 1 cut(s) 444
Sfr303I CCGCGG 1 cut(s) 166
SgrBI CCGCGG 1 cut(s) 166
SinI GGWCC 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 4 cut(s) 136, 254, 276, 370
SsiI CCGC 5 cut(s) 26, 109, 163, 165, 597
SspMI CTAG 4 cut(s) 213, 501, 528, 634
StyD4I CCNGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 100, 592
TaiI ACGT 1 cut(s) 527
TaqI TCGA 2 cut(s) 359, 569
TasI AATT 4 cut(s) 136, 254, 276, 370
TauI GCSGC 2 cut(s) 165, 168
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 3 cut(s) 15, 279, 518
Tru9I TTAA 3 cut(s) 15, 279, 518
TscAI CASTG 2 cut(s) 96, 595
TseFI GTSAC 1 cut(s) 629
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 3 cut(s) 132, 267, 587
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 2 cut(s) 96, 595
VneI GTGCAC 1 cut(s) 231
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 2 cut(s) 136, 254
XbaI TCTAGA 1 cut(s) 527
XspI CTAG 4 cut(s) 213, 501, 528, 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.