pycom05g32380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013287
Physical Location & Seq
Reverse (-)
31799091 .. 31800748
1658 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32380.2

Sequence Viewer

Length: 606 bp
ATGCTAAAGATGGCTCTGGTTCTTAACCTAATCCCGCCATCCCAAGCGCAAACCCTACTCCTTCAGTCGTCGTCGTCGTCCTCCTCCACTCGCCCACTGCCGACTGTCATTTCCCGCCCAAATGAAGCAACCCAAGGCTGGAATTCTCTGCTGTTCAAGCTCAAGTGCCGCGGCAGGTTCTCTTGTCTTTTCTCAGACAATCGCAAGCAGGACCAAGCTAGGAAAGCATTAGAAGGTGCACTTGGTGGAAAGAAAGATGAATTCAAGAAATGGGACAAAGAAATTAAGAGAAGGGAGGAGGTGGGTGGAGGAGGTAGCAATGGTGGAGGGGGATGGTTTGGGTGGGGTAGATGGTTTGGATGGTCAAATGATGGCAATTTCTGGCAAGAAGCACAACAAGCAAGTCTTGCTGTGATAGAACTGATGCTTGCTGTCATCTTCAATCCGTTGCTATATGCCTTGCGAGGGACTAGAAATGGGTTTGCTTTAGTAACGTCTAGAATCTTGAGGAAGACAGCACCAGATGGCCATAATATCTCGATGAAGGAAGCCTACGGCACTGTCTCCGCTAAAGAAAGTGTTCTGAGAAAGTGGGGAAGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.27

Weight (kDa)

10.05

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013287)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20130 AT5G20130
fragaria_vesca FvH4_3g00960
malus_domestica MD05G1358000.v1.1 MD10G1331900.v1.1
prunus_persica Prupe.4G010200_v2.0.a1
pyrus_communis pycom05g32380
rosa_chinensis RchiOBHm_Chr5g0001481
rosa_laevigata RLG00000030932
rosa_multiflora Rmu_co8090336.1_g000001 Rmu_sc0003749.1_g000010
rosa_roxburghii Rroxscaffold_1G00074910
rosa_rugosa Rorug04G0390500
rosa_samantha Rh5AG011500 Rh5BG014000 Rh5CG012500 Rh5DG011700
rosa_wichuraiana Rw5G001090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 165
AccII CGCG 1 cut(s) 171
AciI CCGC 5 cut(s) 35, 115, 169, 171, 567
AcoI YGGCCR 1 cut(s) 526
AcsI RAATTY 2 cut(s) 142, 260
AcuI CTGAAG 1 cut(s) 47
AdeI CACNNNGTG 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 138, 465
AgsI TTSAA 3 cut(s) 157, 265, 442
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
AluBI AGCT 2 cut(s) 160, 218
AluI AGCT 2 cut(s) 160, 218
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 1 cut(s) 568
Alw44I GTGCAC 1 cut(s) 237
AoxI GGCC 1 cut(s) 526
ApaLI GTGCAC 1 cut(s) 237
ApoI RAATTY 2 cut(s) 142, 260
Asp700I GAANNNNTTC 1 cut(s) 579
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 211
AvaII GGWCC 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 241
BalI TGGCCA 1 cut(s) 528
BarI GAAGNNNNNNTAC 2 cut(s) 536, 568
BbsI GAAGAC 1 cut(s) 518
Bbv12I GWGCWC 1 cut(s) 241
BccI CCATC 7 cut(s) 4, 46, 327, 345, 354, 365, 518
BceAI ACGGC 1 cut(s) 571
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 4 cut(s) 219, 471, 498, 604
BfuAI ACCTGC 1 cut(s) 165
BisI GCNGC 2 cut(s) 169, 172
BlsI GCNGC 2 cut(s) 170, 173
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmsI GCATC 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 518
BpuEI CTTGAG 2 cut(s) 146, 526
BsaJI CCNNGG 2 cut(s) 133, 169
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 465
Bse3DI GCAATG 1 cut(s) 325
BseDI CCNNGG 2 cut(s) 133, 169
BseGI GGATG 3 cut(s) 38, 338, 365
BseLI CCNNNNNNNGG 2 cut(s) 138, 465
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 2 cut(s) 207, 575
BseRI GAGGAG 3 cut(s) 73, 311, 324
BseSI GKGCMC 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 241
BslFI GGGAC 2 cut(s) 287, 481
BslI CCNNNNNNNGG 2 cut(s) 138, 465
BsmAI GTCTC 1 cut(s) 568
BsmFI GGGAC 2 cut(s) 287, 481
BsnI GGCC 1 cut(s) 528
Bsp1286I GDGCHC 1 cut(s) 241
BspACI CCGC 5 cut(s) 35, 115, 169, 171, 567
BspANI GGCC 1 cut(s) 528
BspCNI CTCAG 2 cut(s) 206, 576
BspFNI CGCG 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 165
BsrDI GCAATG 1 cut(s) 325
BssECI CCNNGG 2 cut(s) 133, 169
BssT1I CCWWGG 1 cut(s) 133
Bst4CI ACNGT 2 cut(s) 106, 562
BstAPI GCANNNNNTGC 1 cut(s) 407
BstC8I GCNNGC 2 cut(s) 206, 429
BstDEI CTNAG 2 cut(s) 193, 584
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 3 cut(s) 38, 338, 365
BstFNI CGCG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 49
BstMAI GTCTC 1 cut(s) 568
BstMWI GCNNNNNNNGC 4 cut(s) 157, 224, 398, 407
BstSLI GKGCMC 1 cut(s) 241
BstUI CGCG 1 cut(s) 171
BstV2I GAAGAC 1 cut(s) 518
BsuRI GGCC 1 cut(s) 528
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 3 cut(s) 38, 338, 365
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 95, 558
BveI ACCTGC 1 cut(s) 165
Cac8I GCNNGC 2 cut(s) 206, 429
CfoI GCGC 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 211
Cfr42I CCGCGG 1 cut(s) 172
CviJI RGCY 6 cut(s) 14, 138, 160, 218, 528, 551
CviKI_1 RGCY 6 cut(s) 14, 138, 160, 218, 528, 551
DdeI CTNAG 2 cut(s) 193, 584
DraIII CACNNNGTG 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 526
Eco130I CCWWGG 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 47
EcoRI GAATTC 2 cut(s) 142, 260
EcoT14I CCWWGG 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 133
FaiI YATR 3 cut(s) 454, 456, 531
FalI AAGNNNNNCTT 4 cut(s) 225, 257, 390, 422
FaqI GGGAC 2 cut(s) 287, 481
FauI CCCGC 2 cut(s) 42, 122
Fnu4HI GCNGC 2 cut(s) 169, 172
FokI GGATG 3 cut(s) 25, 345, 372
Fsp4HI GCNGC 2 cut(s) 169, 172
FspBI CTAG 4 cut(s) 219, 471, 498, 604
GlaI GCGC 1 cut(s) 48
GluI GCNGC 2 cut(s) 169, 172
HaeIII GGCC 1 cut(s) 528
HhaI GCGC 1 cut(s) 49
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HinfI GANTC 1 cut(s) 501
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 2 cut(s) 196, 585
Hpy188III TCNNGA 4 cut(s) 265, 498, 505, 538
Hpy8I GTNNAC 1 cut(s) 239
Hpy99I CGWCG 3 cut(s) 73, 76, 79
HpyAV CCTTC 4 cut(s) 71, 227, 285, 538
HpyCH4III ACNGT 2 cut(s) 106, 562
HpyCH4IV ACGT 1 cut(s) 494
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 4 cut(s) 157, 224, 398, 407
HpyF3I CTNAG 2 cut(s) 193, 584
HpySE526I ACGT 1 cut(s) 494
HspAI GCGC 1 cut(s) 47
KspI CCGCGG 1 cut(s) 172
LpnPI CCDG 6 cut(s) 2, 124, 160, 194, 367, 534
LweI GCATC 1 cut(s) 414
MaeI CTAG 4 cut(s) 219, 471, 498, 604
MaeII ACGT 1 cut(s) 494
MaeIII GTNAC 2 cut(s) 490, 599
MboII GAAGA 2 cut(s) 430, 523
MhlI GDGCHC 1 cut(s) 241
MlsI TGGCCA 1 cut(s) 528
MluCI AATT 4 cut(s) 142, 260, 282, 376
MluNI TGGCCA 1 cut(s) 528
MnlI CCTC 9 cut(s) 91, 94, 289, 292, 302, 305, 320, 458, 501
Mox20I TGGCCA 1 cut(s) 528
MroXI GAANNNNTTC 1 cut(s) 579
MscI TGGCCA 1 cut(s) 528
MseI TTAA 2 cut(s) 24, 285
Msp20I TGGCCA 1 cut(s) 528
MspA1I CMGCKG 1 cut(s) 171
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 4 cut(s) 157, 224, 398, 407
NmuCI GTSAC 1 cut(s) 599
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 1 cut(s) 501
PkrI GCNGC 2 cut(s) 170, 173
PspPI GGNCC 1 cut(s) 211
SacII CCGCGG 1 cut(s) 172
SaqAI TTAA 2 cut(s) 24, 285
SatI GCNGC 2 cut(s) 169, 172
Sau96I GGNCC 1 cut(s) 211
SduI GDGCHC 1 cut(s) 241
SetI ASST 8 cut(s) 30, 162, 179, 220, 238, 303, 316, 497
SfaNI GCATC 1 cut(s) 414
Sfr303I CCGCGG 1 cut(s) 172
SgrBI CCGCGG 1 cut(s) 172
SinI GGWCC 1 cut(s) 211
SmlI CTYRAG 2 cut(s) 161, 505
SmoI CTYRAG 2 cut(s) 161, 505
Sse9I AATT 4 cut(s) 142, 260, 282, 376
SsiI CCGC 5 cut(s) 35, 115, 169, 171, 567
SspMI CTAG 4 cut(s) 219, 471, 498, 604
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 106, 562
TaiI ACGT 1 cut(s) 497
TaqI TCGA 1 cut(s) 539
TasI AATT 4 cut(s) 142, 260, 282, 376
TauI GCSGC 2 cut(s) 171, 174
TfiI GAWTC 1 cut(s) 501
Tru1I TTAA 2 cut(s) 24, 285
Tru9I TTAA 2 cut(s) 24, 285
TscAI CASTG 2 cut(s) 102, 565
TseFI GTSAC 1 cut(s) 599
Tsp45I GTSAC 1 cut(s) 599
TspDTI ATGAA 3 cut(s) 138, 273, 557
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 2 cut(s) 102, 565
VneI GTGCAC 1 cut(s) 237
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 2 cut(s) 142, 260
XbaI TCTAGA 1 cut(s) 497
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 4 cut(s) 219, 471, 498, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.