MD10G1331900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013287
Physical Location & Seq
Reverse (-)
40950278 .. 40952280
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1331900.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGCGCTTGTTCTTAACCTAATTCCGCCATCCCAAACCCTCTTTCAGTCATCGTCCTCCTCCACTCGCCCACTTCCGACTGTCATTTCCCGCCCAAATGAAGCAACTCAGGACTGGAATTCTCTTCTGTTCAAGCTCAAGTGCCGCGGCAGGTTCTCGTGCCTTTTCTCAGACAATAGCAAACAGGACCAAGCTAGGAAAGCTTTAGAAGGTGCACTTGGTGGAAAGAAAGATGAATTCAAGAAATGGGACAAAGAAATAAAGAGAAGAGAGGAGGTGGGTGGAGGCGGTAGCACTGGTGGAGGGGGATGGTTTGGGTGGGGTAGATGGTTTGGATGGTCGAATGACGGCAATTTCTGGCAAGAAGCACAACAAGCAAGTCTTGCTGTGATAGGTATCATCTTGATGTACCTCATAATTGCAAAGGGAGAACTGATGCTTGCTGTCATCTTCAATCCGTTGCTATACGCCTTGCGAGGGACAAGAAGTGGGTTTGCTTTCATAACTTCCAGAATCTTAAGGAAGAGAGCGCCAGATGGCCATAATATCTCGATGAAGGAAGCCTACGGCGGTGTCTCCGCTAAAGAGAGTGTTCTGAGAAAATGGGGAAGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

206

Amino Acids

22.74

Weight (kDa)

9.91

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013287)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20130 AT5G20130
fragaria_vesca FvH4_3g00960
malus_domestica MD05G1358000.v1.1 MD10G1331900.v1.1
prunus_persica Prupe.4G010200_v2.0.a1
pyrus_communis pycom05g32380
rosa_chinensis RchiOBHm_Chr5g0001481
rosa_laevigata RLG00000030932
rosa_multiflora Rmu_co8090336.1_g000001 Rmu_sc0003749.1_g000010
rosa_roxburghii Rroxscaffold_1G00074910
rosa_rugosa Rorug04G0390500
rosa_samantha Rh5AG011500 Rh5BG014000 Rh5CG012500 Rh5DG011700
rosa_wichuraiana Rw5G001090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 141
AccII CGCG 1 cut(s) 147
AciI CCGC 7 cut(s) 26, 91, 145, 147, 288, 570, 579
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 2 cut(s) 118, 236
AdeI CACNNNGTG 1 cut(s) 221
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 1 cut(s) 477
AflII CTTAAG 1 cut(s) 517
AgsI TTSAA 3 cut(s) 133, 241, 454
AjuI GAANNNNNNNTTGG 4 cut(s) 27, 59, 201, 233
AluBI AGCT 3 cut(s) 136, 194, 203
AluI AGCT 3 cut(s) 136, 194, 203
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 1 cut(s) 580
Alw44I GTGCAC 1 cut(s) 213
AoxI GGCC 1 cut(s) 538
ApaLI GTGCAC 1 cut(s) 213
ApoI RAATTY 2 cut(s) 118, 236
AspLEI GCGC 2 cut(s) 7, 532
AspS9I GGNCC 1 cut(s) 187
AvaII GGWCC 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 540
BarI GAAGNNNNNNTAC 2 cut(s) 548, 580
BauI CACGAG 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 217
BccI CCATC 5 cut(s) 37, 303, 321, 330, 530
BceAI ACGGC 2 cut(s) 364, 583
BcoDI GTCTC 1 cut(s) 580
BfaI CTAG 2 cut(s) 195, 616
BfoI RGCGCY 2 cut(s) 8, 533
BfrI CTTAAG 1 cut(s) 517
BfuAI ACCTGC 1 cut(s) 141
BisI GCNGC 2 cut(s) 145, 148
BlsI GCNGC 2 cut(s) 146, 149
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 187
BmsI GCATC 1 cut(s) 426
BpuEI CTTGAG 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 395
BsaJI CCNNGG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse1I ACTGG 2 cut(s) 119, 301
Bse8I GATNNNNATC 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 3 cut(s) 29, 314, 341
BseJI GATNNNNATC 1 cut(s) 395
BseLI CCNNNNNNNGG 1 cut(s) 477
BseMII CTCAG 3 cut(s) 122, 183, 587
BseNI ACTGG 2 cut(s) 119, 301
BseRI GAGGAG 2 cut(s) 49, 287
BseSI GKGCMC 1 cut(s) 217
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 540
BsiHKAI GWGCWC 1 cut(s) 217
BslFI GGGAC 2 cut(s) 263, 493
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 1 cut(s) 580
BsmFI GGGAC 2 cut(s) 263, 493
BsnI GGCC 1 cut(s) 540
Bsp1286I GDGCHC 1 cut(s) 217
BspACI CCGC 7 cut(s) 26, 91, 145, 147, 288, 570, 579
BspANI GGCC 1 cut(s) 540
BspCNI CTCAG 3 cut(s) 121, 182, 588
BspFNI CGCG 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 141
BspTI CTTAAG 1 cut(s) 517
BsrI ACTGG 2 cut(s) 119, 301
BssECI CCNNGG 1 cut(s) 145
BssSI CACGAG 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 157
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 3 cut(s) 129, 262, 518
BstAFI CTTAAG 1 cut(s) 517
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 3 cut(s) 108, 169, 596
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 3 cut(s) 29, 314, 341
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 2 cut(s) 8, 533
BstHHI GCGC 2 cut(s) 7, 532
BstMAI GTCTC 1 cut(s) 580
BstMWI GCNNNNNNNGC 3 cut(s) 200, 374, 383
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 1 cut(s) 147
BsuRI GGCC 1 cut(s) 540
BtgI CCRYGG 1 cut(s) 145
BtsCI GGATG 3 cut(s) 29, 314, 341
BtsIMutI CAGTG 1 cut(s) 294
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 1 cut(s) 441
CfoI GCGC 2 cut(s) 7, 532
Cfr13I GGNCC 1 cut(s) 187
Cfr42I CCGCGG 1 cut(s) 148
Csp6I GTAC 1 cut(s) 409
CviJI RGCY 5 cut(s) 136, 194, 203, 540, 563
CviKI_1 RGCY 5 cut(s) 136, 194, 203, 540, 563
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 3 cut(s) 108, 169, 596
DraIII CACNNNGTG 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 3 cut(s) 129, 262, 518
EarI CTCTTC 3 cut(s) 129, 262, 518
EciI GGCGGA 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 187
EcoRI GAATTC 2 cut(s) 118, 236
FaiI YATR 4 cut(s) 416, 466, 503, 543
FalI AAGNNNNNCTT 4 cut(s) 201, 233, 366, 398
FaqI GGGAC 2 cut(s) 263, 493
FauI CCCGC 1 cut(s) 98
Fnu4HI GCNGC 2 cut(s) 145, 148
FokI GGATG 3 cut(s) 16, 321, 348
Fsp4HI GCNGC 2 cut(s) 145, 148
FspBI CTAG 2 cut(s) 195, 616
GlaI GCGC 2 cut(s) 6, 531
GluI GCNGC 2 cut(s) 145, 148
HaeII RGCGCY 2 cut(s) 8, 533
HaeIII GGCC 1 cut(s) 540
HhaI GCGC 2 cut(s) 7, 532
Hin6I GCGC 2 cut(s) 5, 530
HinP1I GCGC 2 cut(s) 5, 530
HindIII AAGCTT 1 cut(s) 201
HinfI GANTC 1 cut(s) 513
Hpy166II GTNNAC 1 cut(s) 215
Hpy188I TCNGA 3 cut(s) 78, 172, 597
Hpy188III TCNNGA 5 cut(s) 110, 241, 403, 510, 550
Hpy8I GTNNAC 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 203, 550
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4V TGCA 2 cut(s) 215, 422
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 374, 383
HpyF3I CTNAG 3 cut(s) 108, 169, 596
HspAI GCGC 2 cut(s) 5, 530
KspI CCGCGG 1 cut(s) 148
LpnPI CCDG 8 cut(s) 95, 100, 136, 170, 282, 343, 523, 546
LweI GCATC 1 cut(s) 426
MaeI CTAG 2 cut(s) 195, 616
MaeIII GTNAC 1 cut(s) 611
MboII GAAGA 4 cut(s) 116, 279, 442, 535
MhlI GDGCHC 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 540
MluCI AATT 5 cut(s) 21, 118, 236, 352, 417
MluNI TGGCCA 1 cut(s) 540
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 9 cut(s) 50, 67, 70, 265, 268, 278, 296, 422, 470
Mox20I TGGCCA 1 cut(s) 540
MscI TGGCCA 1 cut(s) 540
MseI TTAA 2 cut(s) 15, 518
MslI CAYNNNNRTG 1 cut(s) 404
Msp20I TGGCCA 1 cut(s) 540
MspA1I CMGCKG 1 cut(s) 147
MspCI CTTAAG 1 cut(s) 517
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 3 cut(s) 200, 374, 383
NmuCI GTSAC 1 cut(s) 611
PfeI GAWTC 1 cut(s) 513
PkrI GCNGC 2 cut(s) 146, 149
PspPI GGNCC 1 cut(s) 187
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 404
SacII CCGCGG 1 cut(s) 148
SaqAI TTAA 2 cut(s) 15, 518
SatI GCNGC 2 cut(s) 145, 148
Sau96I GGNCC 1 cut(s) 187
SduI GDGCHC 1 cut(s) 217
SetI ASST 9 cut(s) 21, 138, 155, 196, 205, 214, 279, 397, 414
SfaNI GCATC 1 cut(s) 426
Sfr303I CCGCGG 1 cut(s) 148
SgrBI CCGCGG 1 cut(s) 148
SinI GGWCC 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 2 cut(s) 137, 517
SmoI CTYRAG 2 cut(s) 137, 517
Sse9I AATT 5 cut(s) 21, 118, 236, 352, 417
SsiI CCGC 7 cut(s) 26, 91, 145, 147, 288, 570, 579
SspMI CTAG 2 cut(s) 195, 616
TaaI ACNGT 1 cut(s) 82
TaqI TCGA 2 cut(s) 341, 551
TasI AATT 5 cut(s) 21, 118, 236, 352, 417
TauI GCSGC 2 cut(s) 147, 150
TfiI GAWTC 1 cut(s) 513
Tru1I TTAA 2 cut(s) 15, 518
Tru9I TTAA 2 cut(s) 15, 518
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 1 cut(s) 611
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 4 cut(s) 114, 249, 490, 569
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 517
VneI GTGCAC 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 2 cut(s) 118, 236
XspI CTAG 2 cut(s) 195, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.