Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004756

Physical Location & Seq

Genomic Coordinates Reverse (-)

47935734 .. 47944481

Gene Structure 8748 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1364500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGATCCATCAGTTCATTAATTTTGTCATTCGCCCTCCCAGGGCAGAGTATAACCCAGATCAATATCTTTGGGAAAAGGATTTCACGCTTGCAGGTAGAGCATACAAACGACAAGACTTGGAGCTTACGAATGCTAGGGGCCATACCTTGCGTTGTAGTCATTATCTGCCTTCACCTTTCCCTGAAGATGTTTCTCTTCCATGTGTTATATACTGCCATGGAAACAGTGGGTGTAGGGCAGATGCAAATGAAGCTGCCGTAATGCTTCTTCCATCAAATATCACCGTTTTTACTCTTGATTTTTCGGGTTCAGGATTATCTGATGGTGATTATGTCAGCCTTGGTTGGCATGAGAGAGATGACCTCAAGATTGTGGTTTCATATTTAAGAAGCAAAAAGCAAAACTTGCGTATAGGTCTTTGGGGGCGATCGATGGGCGCAGTCACATGCCTACTTTATGGAGCAGAAGACCCTTCCATAGCCGGAATGGTGTTGGATAGTGCCTTTTCAAGCTTGTATGTTCTAATGATGGAACTAGTTGATGTATACAAGATTCGGCTTCCTAAATTTACAGTTAAGATGGCAGTACAATATATGCGTCGGATAATTGAGAAGAAGGCAAAGTTTGATATCATGGATCTTAATTGCTTGCAGGTTGCACCCAAAACATTCATTCCTGCTTTATTTGGACATGCTAAGGATGACAAATTCATCCAAACCCATCATTCTGACATCATCTACAAGTCTTATGCGGGGGACAAAAATATTATACATTTCGATGGTGATCACAACTCTTCTCGGCCACAGTTTTATTATGATTCAGTTTCCATTTTCTTCTATAATGTTCTTCATCCCCCACAAACGTCTTGTCATTCATGTAAGCTTGAGAAGTATTATGATTTGGGGGATTTGAAGATTGGTGCTGGTTTGGATGAGGGTTTGTTATATGAGATAATTACTGGAGTTCATTCTGCGGGTACTGACGTGGCAAGTTCATCTTCTGTTCCTCCTGCCATTTCAACCACAAAATGTGTTGGCGAACTTCTTTCTGAAATTGCACCAGTGACGACTGCTGTTGATTCTGTGCATGATGAAGCTGACACACTTAACAGTCACGAACCATCACATTTAGAGGAGCAGCCTAATGATGAGAATGTAGAATGTTGTTCATATACAAGCTCAAACAGGGAGAGTTGGGGCAGATGCTCTTCATTAGGAGGCAGTGATGAAGAATCTTCTGCTGCTGACAGCAGTCATCAGAAGAACCTTAAGGTGTTTGCGATGCCTCTTGGATGCATACAACAAAAATCATCAGAGCTAAGAAAAGAGGAAAAAAAGAAGAAGAAAGTTTCCATTGCTCCAAAGAAGCCCAAGGGTGAGAAATTTGAGAAGTTAGGGGCCCTTGGCAAACGACTGCGTCTTTGCATCCTAAAGCGAGTAAAAAAATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_4

Protein Analysis

484

Amino Acids

54.03

Weight (kDa)

6.98

Isoelectric Point (pI)

49.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	67 - 171	1.6e-06	alpha/beta hydrolase fold
Hydrolase_4	PF12146	68 - 175	6.2e-10	Serine aminopeptidase, S33
Peptidase_S15	PF02129	95 - 175	1e-05	X-Pro dipeptidyl-peptidase (S15 family)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17150 AT4G17150 AT4G17150 AT4G17150 AT4G17150
fragaria_vesca	FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060
malus_domestica	MD05G1364500.v1.1
prunus_persica	Prupe.4G000500_v2.0.a1 Prupe.4G000500_v2.0.a1
pyrus_communis	pycom05g33060 pycom10g29160
rosa_chinensis	RchiOBHm_Chr5g0000061
rosa_laevigata	RLG00000030840
rosa_multiflora	Rmu_ssc0000243.1_g000025
rosa_roxburghii	Rroxscaffold_1G00076010
rosa_rugosa	Rorug04G0381000 Rorug04G0381100 Rorug04G0381200
rosa_samantha	Rh5AG000500 Rh5BG001700 Rh5CG000600 Rh5DG000500
rosa_wichuraiana	Rw5G000080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	83, 643
AccI	GTMKAC	1 cut(s)	546
AciI	CCGC	2 cut(s)	752, 974
AclWI	GGATC	1 cut(s)	645
AcoI	YGGCCR	1 cut(s)	800
AcsI	RAATTY	3 cut(s)	566, 707, 1382
AcuI	CTGAAG	1 cut(s)	204
AfaI	GTAC	2 cut(s)	588, 979
AfiI	CCNNNNNNNGG	1 cut(s)	40
AflII	CTTAAG	1 cut(s)	1268
AgsI	TTSAA	3 cut(s)	510, 913, 1020
AhlI	ACTAGT	1 cut(s)	535
AjiI	CACGTC	1 cut(s)	985
AjnI	CCWGG	1 cut(s)	38
AluBI	AGCT	7 cut(s)	124, 254, 513, 883, 1097, 1179, 1318
AluI	AGCT	7 cut(s)	124, 254, 513, 883, 1097, 1179, 1318
AlwI	GGATC	1 cut(s)	645
AoxI	GGCC	3 cut(s)	139, 800, 1398
ApaI	GGGCCC	1 cut(s)	1402
ApeKI	GCWGC	3 cut(s)	254, 1138, 1241
ApoI	RAATTY	3 cut(s)	566, 707, 1382
ArsI	GACNNNNNNTTYG	4 cut(s)	853, 885, 1404, 1436
AseI	ATTAAT	1 cut(s)	18
AspLEI	GCGC	1 cut(s)	440
AspS9I	GGNCC	3 cut(s)	139, 1398, 1399
AsuHPI	GGTGA	5 cut(s)	165, 274, 338, 794, 1388
BaeGI	GKGCMC	1 cut(s)	1402
BaeI	ACNNNNGTAYC	2 cut(s)	969, 1002
BanII	GRGCYC	1 cut(s)	1402
BarI	GAAGNNNNNNTAC	2 cut(s)	252, 284
BbsI	GAAGAC	1 cut(s)	474
BbvI	GCAGC	3 cut(s)	241, 1150, 1228
BccI	CCATC	9 cut(s)	15, 280, 317, 427, 523, 574, 729, 773, 1129
BceAI	ACGGC	1 cut(s)	242
BciT130I	CCWGG	1 cut(s)	40
BclI	TGATCA	1 cut(s)	784
BcuI	ACTAGT	1 cut(s)	535
BfaI	CTAG	2 cut(s)	135, 536
BfrI	CTTAAG	1 cut(s)	1268
BfuAI	ACCTGC	2 cut(s)	83, 643
BisI	GCNGC	3 cut(s)	255, 1139, 1242
BlsI	GCNGC	3 cut(s)	256, 1140, 1243
Bme1390I	CCNGG	1 cut(s)	40
BmgBI	CACGTC	1 cut(s)	985
BmgT120I	GGNCC	3 cut(s)	139, 1398, 1399
BmiI	GGNNCC	3 cut(s)	140, 1399, 1400
BmrFI	CCNGG	1 cut(s)	40
BmsI	GCATC	5 cut(s)	232, 1193, 1272, 1283, 1434
BoxI	GACNNNNGTC	1 cut(s)	1251
BpiI	GAAGAC	1 cut(s)	474
BplI	GAGNNNNNCTC	2 cut(s)	348, 380
BpmI	CTGGAG	1 cut(s)	981
Bpu10I	CCTNAGC	1 cut(s)	696
BpuEI	CTTGAG	2 cut(s)	350, 905
Bsa29I	ATCGAT	1 cut(s)	431
BsaBI	GATNNNNATC	2 cut(s)	63, 783
BsaJI	CCNNGG	6 cut(s)	38, 39, 217, 340, 1371, 1402
Bsc4I	CCNNNNNNNGG	1 cut(s)	40
Bse1I	ACTGG	2 cut(s)	964, 1061
Bse3DI	GCAATG	1 cut(s)	1353
Bse8I	GATNNNNATC	2 cut(s)	63, 783
BseBI	CCWGG	1 cut(s)	40
BseCI	ATCGAT	1 cut(s)	431
BseDI	CCNNGG	6 cut(s)	38, 39, 217, 340, 1371, 1402
BseGI	GGATG	6 cut(s)	706, 711, 850, 937, 1298, 1425
BseJI	GATNNNNATC	2 cut(s)	63, 783
BseLI	CCNNNNNNNGG	1 cut(s)	40
BseMI	GCAATG	1 cut(s)	1353
BseNI	ACTGG	2 cut(s)	964, 1061
BseRI	GAGGAG	1 cut(s)	1148
BseSI	GKGCMC	1 cut(s)	1402
BseXI	GCAGC	3 cut(s)	241, 1150, 1228
Bsh1285I	CGRYCG	1 cut(s)	431
BshFI	GGCC	3 cut(s)	141, 802, 1400
BshVI	ATCGAT	1 cut(s)	431
BsiEI	CGRYCG	1 cut(s)	431
BsiSI	CCGG	1 cut(s)	483
BslFI	GGGAC	1 cut(s)	770
BslI	CCNNNNNNNGG	1 cut(s)	40
BsmFI	GGGAC	1 cut(s)	770
BsmI	GAATGC	1 cut(s)	136
BsnI	GGCC	3 cut(s)	141, 802, 1400
Bsp120I	GGGCCC	1 cut(s)	1398
Bsp1286I	GDGCHC	1 cut(s)	1402
Bsp143I	GATC	5 cut(s)	3, 58, 428, 637, 784
Bsp19I	CCATGG	1 cut(s)	217
BspACI	CCGC	2 cut(s)	752, 974
BspANI	GGCC	3 cut(s)	141, 802, 1400
BspDI	ATCGAT	1 cut(s)	431
BspLI	GGNNCC	3 cut(s)	140, 1399, 1400
BspMI	ACCTGC	2 cut(s)	83, 643
BspPI	GGATC	1 cut(s)	645
BspQI	GCTCTTC	1 cut(s)	1213
BspTI	CTTAAG	1 cut(s)	1268
BsrDI	GCAATG	1 cut(s)	1353
BsrI	ACTGG	2 cut(s)	964, 1061
BssECI	CCNNGG	6 cut(s)	38, 39, 217, 340, 1371, 1402
BssMI	GATC	5 cut(s)	3, 58, 428, 637, 784
BssNAI	GTATAC	1 cut(s)	547
BssT1I	CCWWGG	4 cut(s)	217, 340, 1371, 1402
Bst1107I	GTATAC	1 cut(s)	547
Bst2UI	CCWGG	1 cut(s)	40
Bst4CI	ACNGT	5 cut(s)	227, 286, 574, 807, 1112
Bst6I	CTCTTC	3 cut(s)	201, 799, 1213
BstAFI	CTTAAG	1 cut(s)	1268
BstAPI	GCANNNNNTGC	1 cut(s)	406
BstC8I	GCNNGC	2 cut(s)	90, 650
BstDEI	CTNAG	2 cut(s)	696, 1319
BstDSI	CCRYGG	1 cut(s)	217
BstF5I	GGATG	6 cut(s)	706, 711, 850, 937, 1298, 1425
BstHHI	GCGC	1 cut(s)	440
BstKTI	GATC	5 cut(s)	6, 61, 431, 640, 787
BstMBI	GATC	5 cut(s)	3, 58, 428, 637, 784
BstMCI	CGRYCG	1 cut(s)	431
BstMWI	GCNNNNNNNGC	3 cut(s)	98, 251, 406
BstNI	CCWGG	1 cut(s)	40
BstNSI	RCATGY	2 cut(s)	450, 695
BstPAI	GACNNNNGTC	1 cut(s)	1251
BstSCI	CCNGG	1 cut(s)	38
BstSLI	GKGCMC	1 cut(s)	1402
BstV1I	GCAGC	3 cut(s)	241, 1150, 1228
BstV2I	GAAGAC	1 cut(s)	474
BstX2I	RGATCY	1 cut(s)	637
BstYI	RGATCY	1 cut(s)	637
BstZ17I	GTATAC	1 cut(s)	547
Bsu15I	ATCGAT	1 cut(s)	431
BsuRI	GGCC	3 cut(s)	141, 802, 1400
BsuTUI	ATCGAT	1 cut(s)	431
BtgI	CCRYGG	1 cut(s)	217
BtgZI	GCGATG	1 cut(s)	1295
BtrI	CACGTC	1 cut(s)	985
BtsCI	GGATG	6 cut(s)	706, 711, 850, 937, 1298, 1425
BtsI	GCAGTG	1 cut(s)	1228
BtsIMutI	CAGTG	3 cut(s)	232, 1068, 1228
BveI	ACCTGC	2 cut(s)	83, 643
Cac8I	GCNNGC	2 cut(s)	90, 650
CfoI	GCGC	1 cut(s)	440
Cfr13I	GGNCC	3 cut(s)	139, 1398, 1399
ClaI	ATCGAT	1 cut(s)	431
CseI	GACGC	2 cut(s)	587, 1406
Csp6I	GTAC	2 cut(s)	587, 978
CviAII	CATG	8 cut(s)	201, 218, 350, 447, 634, 692, 876, 1088
CviQI	GTAC	2 cut(s)	587, 978
DdeI	CTNAG	2 cut(s)	696, 1319
DpnI	GATC	5 cut(s)	5, 60, 430, 639, 786
DpnII	GATC	5 cut(s)	3, 58, 428, 637, 784
EaeI	YGGCCR	1 cut(s)	800
Eam1104I	CTCTTC	3 cut(s)	201, 799, 1213
EarI	CTCTTC	3 cut(s)	201, 799, 1213
Eco130I	CCWWGG	4 cut(s)	217, 340, 1371, 1402
Eco24I	GRGCYC	1 cut(s)	1402
Eco32I	GATATC	1 cut(s)	631
Eco57I	CTGAAG	1 cut(s)	204
EcoO109I	RGGNCCY	2 cut(s)	1398, 1399
EcoRII	CCWGG	1 cut(s)	38
EcoRV	GATATC	1 cut(s)	631
EcoT14I	CCWWGG	4 cut(s)	217, 340, 1371, 1402
EcoT22I	ATGCAT	1 cut(s)	1298
EcoT38I	GRGCYC	1 cut(s)	1402
ErhI	CCWWGG	4 cut(s)	217, 340, 1371, 1402
FaeI	CATG	8 cut(s)	204, 221, 353, 450, 637, 695, 879, 1091
FalI	AAGNNNNNCTT	4 cut(s)	389, 421, 982, 1014
FaqI	GGGAC	1 cut(s)	770
FatI	CATG	8 cut(s)	200, 217, 349, 446, 633, 691, 875, 1087
FauI	CCCGC	2 cut(s)	745, 967
FbaI	TGATCA	1 cut(s)	784
FblI	GTMKAC	1 cut(s)	546
Fnu4HI	GCNGC	3 cut(s)	255, 1139, 1242
FokI	GGATG	6 cut(s)	698, 713, 837, 944, 1305, 1412
FriOI	GRGCYC	1 cut(s)	1402
Fsp4HI	GCNGC	3 cut(s)	255, 1139, 1242
FspBI	CTAG	2 cut(s)	135, 536
GlaI	GCGC	1 cut(s)	439
GluI	GCNGC	3 cut(s)	255, 1139, 1242
GsuI	CTGGAG	1 cut(s)	981
HaeIII	GGCC	3 cut(s)	141, 802, 1400
HapII	CCGG	1 cut(s)	483
HgaI	GACGC	2 cut(s)	587, 1406
HhaI	GCGC	1 cut(s)	440
Hin1II	CATG	8 cut(s)	204, 221, 353, 450, 637, 695, 879, 1091
Hin6I	GCGC	1 cut(s)	438
HinP1I	GCGC	1 cut(s)	438
HindIII	AAGCTT	2 cut(s)	511, 881
HinfI	GANTC	4 cut(s)	553, 818, 1079, 1232
HpaII	CCGG	1 cut(s)	483
HphI	GGTGA	5 cut(s)	165, 274, 338, 794, 1388
Hpy166II	GTNNAC	1 cut(s)	547
Hpy188I	TCNGA	6 cut(s)	322, 603, 730, 1051, 1260, 1315
Hpy188III	TCNNGA	4 cut(s)	296, 312, 367, 1115
Hpy8I	GTNNAC	1 cut(s)	547
Hpy99I	CGWCG	1 cut(s)	603
HpyAV	CCTTC	3 cut(s)	180, 483, 610
HpyCH4III	ACNGT	5 cut(s)	227, 286, 574, 807, 1112
HpyCH4IV	ACGT	2 cut(s)	863, 984
HpyCH4V	TGCA	8 cut(s)	92, 245, 652, 659, 1058, 1087, 1296, 1425
HpyF10VI	GCNNNNNNNGC	3 cut(s)	98, 251, 406
HpyF3I	CTNAG	2 cut(s)	696, 1319
HpySE526I	ACGT	2 cut(s)	863, 984
Hsp92II	CATG	8 cut(s)	204, 221, 353, 450, 637, 695, 879, 1091
HspAI	GCGC	1 cut(s)	438
Ksp22I	TGATCA	1 cut(s)	784
Kzo9I	GATC	5 cut(s)	3, 58, 428, 637, 784
LguI	GCTCTTC	1 cut(s)	1213
LmnI	GCTCC	4 cut(s)	121, 461, 1135, 1363
Lsp1109I	GCAGC	3 cut(s)	241, 1150, 1228
LweI	GCATC	5 cut(s)	232, 1193, 1272, 1283, 1434
MaeI	CTAG	2 cut(s)	135, 536
MaeII	ACGT	2 cut(s)	863, 984
MaeIII	GTNAC	3 cut(s)	442, 1063, 1112
MalI	GATC	5 cut(s)	5, 60, 430, 639, 786
MboI	GATC	5 cut(s)	3, 58, 428, 637, 784
MflI	RGATCY	1 cut(s)	637
MhlI	GDGCHC	1 cut(s)	1402
MluCI	AATT	9 cut(s)	19, 566, 606, 643, 707, 954, 1053, 1382, 1445
MmeI	TCCRAC	2 cut(s)	474, 581
MnlI	CCTC	8 cut(s)	45, 374, 928, 1017, 1126, 1211, 1296, 1321
Mph1103I	ATGCAT	1 cut(s)	1298
MseI	TTAA	6 cut(s)	18, 386, 576, 642, 1107, 1269
MslI	CAYNNNNRTG	1 cut(s)	777
MspCI	CTTAAG	1 cut(s)	1268
MspI	CCGG	1 cut(s)	483
MspR9I	CCNGG	1 cut(s)	40
Mva1269I	GAATGC	1 cut(s)	136
MvaI	CCWGG	1 cut(s)	40
MwoI	GCNNNNNNNGC	3 cut(s)	98, 251, 406
NcoI	CCATGG	1 cut(s)	217
NdeII	GATC	5 cut(s)	3, 58, 428, 637, 784
NlaIII	CATG	8 cut(s)	204, 221, 353, 450, 637, 695, 879, 1091
NlaIV	GGNNCC	3 cut(s)	140, 1399, 1400
NmeAIII	GCCGAG	1 cut(s)	778
NmuCI	GTSAC	3 cut(s)	442, 1063, 1112
NsiI	ATGCAT	1 cut(s)	1298
NspI	RCATGY	2 cut(s)	450, 695
PasI	CCCWGGG	1 cut(s)	39
PciSI	GCTCTTC	1 cut(s)	1213
PctI	GAATGC	1 cut(s)	136
PfeI	GAWTC	4 cut(s)	553, 818, 1079, 1232
PflFI	GACNNNGTC	1 cut(s)	1416
PkrI	GCNGC	3 cut(s)	256, 1140, 1243
Ple19I	CGATCG	1 cut(s)	431
PshAI	GACNNNNGTC	1 cut(s)	1251
PshBI	ATTAAT	1 cut(s)	18
Psp6I	CCWGG	1 cut(s)	38
PspGI	CCWGG	1 cut(s)	38
PspN4I	GGNNCC	3 cut(s)	140, 1399, 1400
PspOMI	GGGCCC	1 cut(s)	1398
PspPI	GGNCC	3 cut(s)	139, 1398, 1399
PsuI	RGATCY	1 cut(s)	637
PsyI	GACNNNGTC	1 cut(s)	1416
PvuI	CGATCG	1 cut(s)	431
RsaI	GTAC	2 cut(s)	588, 979
RsaNI	GTAC	2 cut(s)	587, 978
RseI	CAYNNNNRTG	1 cut(s)	777
SapI	GCTCTTC	1 cut(s)	1213
SaqAI	TTAA	6 cut(s)	18, 386, 576, 642, 1107, 1269
SatI	GCNGC	3 cut(s)	255, 1139, 1242
Sau3AI	GATC	5 cut(s)	3, 58, 428, 637, 784
Sau96I	GGNCC	3 cut(s)	139, 1398, 1399
ScrFI	CCNGG	1 cut(s)	40
SduI	GDGCHC	1 cut(s)	1402
SfaNI	GCATC	5 cut(s)	232, 1193, 1272, 1283, 1434
SmiMI	CAYNNNNRTG	1 cut(s)	777
SmlI	CTYRAG	3 cut(s)	365, 884, 1268
SmoI	CTYRAG	3 cut(s)	365, 884, 1268
SpeI	ACTAGT	1 cut(s)	535
Sse9I	AATT	9 cut(s)	19, 566, 606, 643, 707, 954, 1053, 1382, 1445
SsiI	CCGC	2 cut(s)	752, 974
SspI	AATATT	1 cut(s)	766
SspMI	CTAG	2 cut(s)	135, 536
StyD4I	CCNGG	1 cut(s)	38
StyI	CCWWGG	4 cut(s)	217, 340, 1371, 1402
TaaI	ACNGT	5 cut(s)	227, 286, 574, 807, 1112
TaiI	ACGT	2 cut(s)	866, 987
TaqI	TCGA	2 cut(s)	431, 777
TasI	AATT	9 cut(s)	19, 566, 606, 643, 707, 954, 1053, 1382, 1445
TatI	WGTACW	1 cut(s)	586
TfiI	GAWTC	4 cut(s)	553, 818, 1079, 1232
Tru1I	TTAA	6 cut(s)	18, 386, 576, 642, 1107, 1269
Tru9I	TTAA	6 cut(s)	18, 386, 576, 642, 1107, 1269
TscAI	CASTG	3 cut(s)	232, 1068, 1228
TseFI	GTSAC	3 cut(s)	442, 1063, 1112
TseI	GCWGC	3 cut(s)	254, 1138, 1241
Tsp45I	GTSAC	3 cut(s)	442, 1063, 1112
TspRI	CASTG	3 cut(s)	232, 1068, 1228
Tth111I	GACNNNGTC	1 cut(s)	1416
Vha464I	CTTAAG	1 cut(s)	1268
VspI	ATTAAT	1 cut(s)	18
XapI	RAATTY	3 cut(s)	566, 707, 1382
XceI	RCATGY	2 cut(s)	450, 695
XcmI	CCANNNNNNNNNTGG	2 cut(s)	224, 484
XmiI	GTMKAC	1 cut(s)	546
XspI	CTAG	2 cut(s)	135, 536
Zsp2I	ATGCAT	1 cut(s)	1298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.