Rorug04G0381200

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004756
Physical Location & Seq
Reverse (-)
55155521 .. 55164025
8505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0381200.1

Sequence Viewer

Length: 381 bp
ATGAAATCATTTGATAATCTAAACAACTGGAGAGAAGAATTTCTGATTCAGGCTAGTCCATCCGATCCCGAGAACTTTCCATTCGTTGTGTTGGGGAACAAGATTGATGTTGATGGTGGAAATAGTCGAGTGGTTTCTGAAAAGAAAGTGAAGGCCTGGTGTGCTTCCAAGGGAAACATACCATACTTTGAGACCTCTGCAAAAGAAGGCTTTAACGTGGATGATGACTTCCAATGTATAGCGAAAAACGCACTGAAGAATGAACCTGAAGAAGAAATGCATAATTGGGACCTGGTGTGGGATGCAACTGCAGCTGATCCTAAATTGCTGGTCTTTTTGAAATCGTACAGGAATTTCTTACACATTGAATCTGGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

126

Amino Acids

14.37

Weight (kDa)

4.78

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 2 - 84 1.8e-14 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 59, 311
AcsI RAATTY 2 cut(s) 38, 352
AcuI CTGAAG 2 cut(s) 275, 288
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 1 cut(s) 298
AgsI TTSAA 2 cut(s) 340, 368
AjnI CCWGG 2 cut(s) 155, 291
AluBI AGCT 1 cut(s) 314
AluI AGCT 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 185
AlwI GGATC 2 cut(s) 59, 311
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 38, 352
Asp700I GAANNNNTTC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 289
AvaI CYCGRG 1 cut(s) 68
AvaII GGWCC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 323
BccI CCATC 2 cut(s) 67, 107
BciT130I CCWGG 2 cut(s) 157, 293
BcoDI GTCTC 1 cut(s) 185
BfaI CTAG 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 309
BisI GCNGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 2 cut(s) 157, 293
Bme18I GGWCC 1 cut(s) 289
BmeT110I CYCGRG 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 290
BmrFI CCNGG 2 cut(s) 157, 293
BmsI GCATC 1 cut(s) 292
BpmI CTGGAG 1 cut(s) 49
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 168
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 32
BseBI CCWGG 2 cut(s) 157, 293
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 3 cut(s) 59, 226, 307
BseLI CCNNNNNNNGG 1 cut(s) 298
BseNI ACTGG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 323
BshFI GGCC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 68
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 1 cut(s) 155
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 68
Bsp143I GATC 2 cut(s) 64, 316
BspANI GGCC 1 cut(s) 155
BspLI GGNNCC 1 cut(s) 290
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 2 cut(s) 59, 311
BspTNI GGTCTC 1 cut(s) 185
BsrI ACTGG 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 2 cut(s) 64, 316
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 2 cut(s) 157, 293
BstF5I GGATG 3 cut(s) 59, 226, 307
BstKTI GATC 2 cut(s) 67, 319
BstMAI GTCTC 1 cut(s) 185
BstMBI GATC 2 cut(s) 64, 316
BstMWI GCNNNNNNNGC 3 cut(s) 161, 248, 311
BstNI CCWGG 2 cut(s) 157, 293
BstSCI CCNGG 2 cut(s) 155, 291
BstSFI CTRYAG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 323
BsuRI GGCC 1 cut(s) 155
BtsCI GGATG 3 cut(s) 59, 226, 307
BtsIMutI CAGTG 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 289
CsiI ACCWGGT 1 cut(s) 291
Csp6I GTAC 1 cut(s) 346
CviJI RGCY 4 cut(s) 53, 155, 210, 314
CviKI_1 RGCY 4 cut(s) 53, 155, 210, 314
CviQI GTAC 1 cut(s) 346
DpnI GATC 2 cut(s) 66, 318
DpnII GATC 2 cut(s) 64, 316
Eco130I CCWWGG 1 cut(s) 168
Eco147I AGGCCT 1 cut(s) 155
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 289
Eco57I CTGAAG 2 cut(s) 275, 288
Eco88I CYCGRG 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 289
EcoRII CCWGG 2 cut(s) 155, 291
EcoT14I CCWWGG 1 cut(s) 168
EcoT22I ATGCAT 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 168
FaiI YATR 4 cut(s) 179, 184, 239, 282
FaqI GGGAC 1 cut(s) 302
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 3 cut(s) 46, 233, 314
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 1 cut(s) 54
GluI GCNGC 1 cut(s) 312
GsuI CTGGAG 1 cut(s) 49
HaeIII GGCC 1 cut(s) 155
HinfI GANTC 2 cut(s) 46, 368
Hpy188I TCNGA 3 cut(s) 45, 64, 139
Hpy188III TCNNGA 1 cut(s) 68
HpyAV CCTTC 2 cut(s) 145, 200
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 4 cut(s) 200, 280, 305, 311
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 248, 311
HpySE526I ACGT 1 cut(s) 216
Kzo9I GATC 2 cut(s) 64, 316
Lsp1109I GCAGC 1 cut(s) 323
LweI GCATC 1 cut(s) 292
MabI ACCWGGT 1 cut(s) 291
MaeI CTAG 1 cut(s) 54
MaeII ACGT 1 cut(s) 216
MalI GATC 2 cut(s) 66, 318
MboI GATC 2 cut(s) 64, 316
MboII GAAGA 4 cut(s) 47, 268, 281, 284
MluCI AATT 5 cut(s) 38, 283, 323, 352, 376
MnlI CCTC 1 cut(s) 205
Mph1103I ATGCAT 1 cut(s) 282
MroXI GAANNNNTTC 1 cut(s) 39
MseI TTAA 1 cut(s) 213
MspA1I CMGCKG 1 cut(s) 314
MspR9I CCNGG 2 cut(s) 157, 293
MvaI CCWGG 2 cut(s) 157, 293
MwoI GCNNNNNNNGC 3 cut(s) 161, 248, 311
NdeII GATC 2 cut(s) 64, 316
NlaIV GGNNCC 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 282
PceI AGGCCT 1 cut(s) 155
PdmI GAANNNNTTC 1 cut(s) 39
PfeI GAWTC 2 cut(s) 46, 368
PkrI GCNGC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 289
Psp5II RGGWCCY 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 155, 291
PspGI CCWGG 2 cut(s) 155, 291
PspN4I GGNNCC 1 cut(s) 290
PspPI GGNCC 1 cut(s) 289
PspPPI RGGWCCY 1 cut(s) 289
PstI CTGCAG 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 2 cut(s) 64, 316
Sau96I GGNCC 1 cut(s) 289
ScrFI CCNGG 2 cut(s) 157, 293
SetI ASST 5 cut(s) 197, 219, 268, 294, 316
SexAI ACCWGGT 1 cut(s) 291
SfaNI GCATC 1 cut(s) 292
SfcI CTRYAG 1 cut(s) 309
SinI GGWCC 1 cut(s) 289
Sse9I AATT 5 cut(s) 38, 283, 323, 352, 376
SseBI AGGCCT 1 cut(s) 155
SspMI CTAG 1 cut(s) 54
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 2 cut(s) 155, 291
StyI CCWWGG 1 cut(s) 168
TaiI ACGT 1 cut(s) 219
TaqI TCGA 1 cut(s) 127
TasI AATT 5 cut(s) 38, 283, 323, 352, 376
TfiI GAWTC 2 cut(s) 46, 368
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 258
TseI GCWGC 1 cut(s) 311
TspDTI ATGAA 2 cut(s) 17, 276
TspRI CASTG 1 cut(s) 258
VpaK11BI GGWCC 1 cut(s) 289
XapI RAATTY 2 cut(s) 38, 352
XmnI GAANNNNTTC 1 cut(s) 39
XspI CTAG 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.