Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0004756

Physical Location & Seq

Genomic Coordinates Forward (+)

55147710 .. 55150264

Gene Structure 2555 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0381000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGGATGGGAAGGCAGCAATATGCCCCTGGTCATTATTAGATGTGGATACACTCAATTTGCCTCGCTCGTATGCCTCTGTGGTTGCGAAACCAGTGGTTTCTGTGGACGACCTGCCAACGCCGGAAATCCATGAAGGCAGAACAACAGTGACAATTTCAGAGGAGGGGTACCGTCTTGGATTGGAGAAGTGTAAACATATGCTTTTGGCACGCCTTCATTTGGCTCCGGGAGAAAAGCACTATTCTCATACCGACCTTCATAAGAAACTGAGCCTTGTCTGGGGAGCGATAGGTCCTTGGCGTTTCATTCCGATGGGTAAGGGGTATTTTACCTTCTGTTTTTCCTCTGAGGAGGTGCTCGCCATGGTATGGGGAATAGGGGCTATTGCGTTAAAGCCTGGAATTCTGAGATTTATGCAGTGGACTCCCAATTTTTCTCCATCCAACCAGAGAAACACTAATGCTCAAGTGTGGGTGCGGCTATGGGACTTGGGTTTAGAATTTTGGGAACCTATTACTCTTTTTGAGATTTCTCATGGAATTGGTGTTCCTATTAAAGTTGATCAGCAAACTTTGGATAGGAGGTGTGGCATGTATGCTAGGGTTTTGGTAGATGTTGATTTGTCCTCTGATCCTCCATGTGAATTGACGGTACGTAGGAAAACTGGGGAGGTTGTGGTGGTGGAGGTTGAGTACGAGCGCCTCCCTGACTTCTGCTATCACTGTGGGAATGTGGGCCATAGGGTTTCTTCATGTAATTTGTTACAGAAATGTACAAATTCAATGCAGCTTGAGGAAACTGAGGGAGCTCGTGGCCGTTCGCGTAAGCCCCGTCGCCGCCGACGTAAAACTAAGCAGGTTTATGTTCAAAAACAGCAGGAAGAACACGCTAATGGGAAGCAAGTGATTGTTGATAATACTCCTCCTCCTGTGATGGATGATCCTAGAGAAGGGCCGTCTTTCACTACGAGGTCTCCCCTTCCTAATCCTATTGCAACACAGCTAATGGGTAATGCAAGTGGTACAGATGGTAACATGGATCTTGCTGTGGAAGTGGAGCTTCCTGCTCCTTCCATTGTGCAACCTGTGGAGCCGGTCATCAGGGAGATACAGAATGTGAATTTACAGATATTAGTGGCTAGTATGCCTTTAATTGAGAAGGTGAGGCCTAATAGGGATAAGGAGCTTGAAGATTCTAGTGAAAATGATAGTGCTCAGAATGTTAATGGAGAGGATGAAGTGGTACCTGAGAATTCAAAGGTGGTTGCCTCCTCTCAAACTTCTCTTTGGGGTATTGCTAATGCCCCTACGCAGAATGCCTTAAAAGATTTTATTCGGGTTCACAAGCCGGAAATACTCTGTATAGCTGAGCCTTTTGTTGCTTTGAACTCTATCCCATCATCATTTTGGCGTGGTTTTGGTTTGGCAGCAATTTGTACTAATGATCGCGGAGGTTCTAGGCCTAACTTATGGTTGTTTGCAGTCTATGCTCGCACTACTATGGCTGAGCGTCGAAGATTATGGTTTGATCTCGCTGAAGTTAAGGGCAGATTTGTTTCAGGGCCATGGTTAGTGTTTGGTGATTTTAATGCGGTGTTGGGAGCTCATGAGAAGAAAGGAGGTGCTCCTGTTTGTCGTCGCTCTTGCGAGGAATTTCAAGCTATGTCTGATATTTGCGAACTAATTCATGTTGATACTAAAGGAGTTGAGTTTACGTGGGCTCGGCGTCGTGGTCTACGGGGTAATGTTGAACTTCGGTTGGATCGATGTCTTGCAAACTTAGAATGGTGTGATGTCTGGAACTCTTTTGATTGTTGTACTTTAGCGCGGATTTGCTCAGATCATAATCCTTTGTTAATGTCTTTTTCAAAAGCTCCTGGGACTTGTCAAAGTATTTTTCGGTTTCGTAAGATGTGGCTGCAGCACAGTGATTTTAAGGAATTTGTTAAGCAATGCTGGTCCTCTATTTCCACTCATGGTTGTCCTCTTTCTGTTCTGCAACATAAGCTGCGTGTTCTGCGTAAAGCACTCAAAGCATGGAATTGGGAAGTCTTTGGGGACATTCACCGGCGTGTGGACGCTGACCTATCTGCTTTAACTATCCTACAAGAGCACATTGCTCATTCTAGTGGGTCGGATGAGGAATTTTCTAAGGAAAATGAACTTCAAGCAAACTTAATTGAGTCTCTTCGGTTGCAAGAGTTATTTTGGAGTGAGAAGTCTCGGTTACGTTGGTTATCTGATGGTGATCGTAATACTACCTACTTTCATGCTATGTGTCGAGCACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_4

Protein Analysis

763

Amino Acids

86.47

Weight (kDa)

7.06

Isoelectric Point (pI)

49.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF4283	PF14111	62 - 148	6.2e-06	Domain of unknown function (DUF4283)
zf-CCHC_4	PF14392	218 - 253	8.7e-09	Zinc knuckle
Exo_endo_phos	PF03372	407 - 615	9e-07	Endonuclease/Exonuclease/phosphatase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17150 AT4G17150 AT4G17150 AT4G17150 AT4G17150
fragaria_vesca	FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060 FvH4_3g00060
malus_domestica	MD05G1364500.v1.1
prunus_persica	Prupe.4G000500_v2.0.a1 Prupe.4G000500_v2.0.a1
pyrus_communis	pycom05g33060 pycom10g29160
rosa_chinensis	RchiOBHm_Chr5g0000061
rosa_laevigata	RLG00000030840
rosa_multiflora	Rmu_ssc0000243.1_g000025
rosa_roxburghii	Rroxscaffold_1G00076010
rosa_rugosa	Rorug04G0381000 Rorug04G0381100 Rorug04G0381200
rosa_samantha	Rh5AG000500 Rh5BG001700 Rh5CG000600 Rh5DG000500
rosa_wichuraiana	Rw5G000080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	120, 847
Acc65I	GGTACC	2 cut(s)	168, 1243
AccB1I	GGYRCC	2 cut(s)	168, 1243
AccB7I	CCANNNNNTGG	1 cut(s)	369
AccI	GTMKAC	1 cut(s)	1735
AccII	CGCG	3 cut(s)	823, 1449, 1828
AciI	CCGC	5 cut(s)	478, 838, 1449, 1592, 1828
AclWI	GGATC	4 cut(s)	626, 935, 1047, 1770
AcoI	YGGCCR	1 cut(s)	814
AcsI	RAATTY	8 cut(s)	402, 500, 778, 1120, 1252, 1652, 1940, 2144
AcuI	CTGAAG	1 cut(s)	1557
AcyI	GRCGYC	1 cut(s)	1726
AfaI	GTAC	8 cut(s)	170, 654, 695, 775, 1024, 1245, 1438, 1819
AfiI	CCNNNNNNNGG	5 cut(s)	220, 280, 369, 950, 2074
AgsI	TTSAA	9 cut(s)	783, 869, 1190, 1257, 1387, 1658, 1751, 1869, 2168
AjnI	CCWGG	3 cut(s)	26, 397, 1876
AleI	CACNNNNGTG	1 cut(s)	2070
AloI	GAACNNNNNNTCC	2 cut(s)	531, 563
Alw21I	GWGCWC	7 cut(s)	360, 811, 1216, 1606, 1627, 2115, 2287
Alw26I	GTCTC	3 cut(s)	978, 2190, 2226
AlwI	GGATC	4 cut(s)	626, 935, 1047, 1770
AoxI	GGCC	6 cut(s)	736, 814, 953, 1166, 1460, 1562
ApeKI	GCWGC	6 cut(s)	14, 787, 1427, 1918, 1921, 2008
ApoI	RAATTY	8 cut(s)	402, 500, 778, 1120, 1252, 1652, 1940, 2144
Asp700I	GAANNNNTTC	1 cut(s)	1683
Asp718I	GGTACC	2 cut(s)	168, 1243
AspLEI	GCGC	2 cut(s)	702, 1828
AspS9I	GGNCC	5 cut(s)	293, 736, 953, 1562, 1959
AsuC2I	CCSGG	1 cut(s)	228
AsuHPI	GGTGA	4 cut(s)	1174, 1592, 2057, 2258
AvaII	GGWCC	2 cut(s)	293, 1959
BanI	GGYRCC	2 cut(s)	168, 1243
BanII	GRGCYC	3 cut(s)	811, 1606, 1723
BarI	GAAGNNNNNNTAC	2 cut(s)	317, 349
BauI	CACGAG	1 cut(s)	810
Bbv12I	GWGCWC	7 cut(s)	360, 811, 1216, 1606, 1627, 2115, 2287
BbvI	GCAGC	6 cut(s)	26, 799, 1439, 1905, 1933, 1995
BccI	CCATC	6 cut(s)	307, 448, 928, 1022, 1405, 2237
BceAI	ACGGC	2 cut(s)	801, 940
BciT130I	CCWGG	3 cut(s)	28, 399, 1878
BciVI	GTATCC	1 cut(s)	40
BclI	TGATCA	1 cut(s)	562
BcnI	CCSGG	1 cut(s)	228
BcoDI	GTCTC	3 cut(s)	978, 2190, 2226
BfaI	CTAG	6 cut(s)	600, 945, 1140, 1197, 1458, 2127
BfmI	CTRYAG	1 cut(s)	1919
BfoI	RGCGCY	1 cut(s)	703
BfuAI	ACCTGC	2 cut(s)	120, 847
BfuI	GTATCC	1 cut(s)	40
BisI	GCNGC	8 cut(s)	15, 479, 788, 838, 1428, 1919, 1922, 2009
BlpI	GCTNAGC	2 cut(s)	1368, 1506
BlsI	GCNGC	8 cut(s)	16, 480, 789, 839, 1429, 1920, 1923, 2010
Bme1390I	CCNGG	4 cut(s)	28, 228, 399, 1878
Bme18I	GGWCC	2 cut(s)	293, 1959
BmgT120I	GGNCC	5 cut(s)	293, 736, 953, 1562, 1959
BmiI	GGNNCC	5 cut(s)	170, 225, 510, 1092, 1245
BmrFI	CCNGG	4 cut(s)	28, 228, 399, 1878
BmrI	ACTGGG	1 cut(s)	675
BmuI	ACTGGG	1 cut(s)	675
Bpu1102I	GCTNAGC	2 cut(s)	1368, 1506
BpuEI	CTTGAG	2 cut(s)	450, 812
BpuMI	CCSGG	1 cut(s)	228
Bsa29I	ATCGAT	1 cut(s)	1765
BsaAI	YACGTR	2 cut(s)	656, 1716
BsaBI	GATNNNNATC	2 cut(s)	1845, 2247
BsaHI	GRCGYC	1 cut(s)	1726
BsaI	GGTCTC	1 cut(s)	978
BsaJI	CCNNGG	5 cut(s)	26, 296, 363, 1565, 1877
BsaXI	ACNNNNNCTCC	6 cut(s)	677, 707, 910, 940, 958, 988
Bsc4I	CCNNNNNNNGG	5 cut(s)	220, 280, 369, 950, 2074
Bse118I	RCCGGY	2 cut(s)	1093, 2067
Bse1I	ACTGG	2 cut(s)	92, 670
Bse3DI	GCAATG	2 cut(s)	1958, 2115
Bse8I	GATNNNNATC	2 cut(s)	1845, 2247
BseBI	CCWGG	3 cut(s)	28, 399, 1878
BseCI	ATCGAT	1 cut(s)	1765
BseDI	CCNNGG	5 cut(s)	26, 296, 363, 1565, 1877
BseGI	GGATG	5 cut(s)	10, 440, 943, 1240, 2143
BseJI	GATNNNNATC	2 cut(s)	1845, 2247
BseLI	CCNNNNNNNGG	5 cut(s)	220, 280, 369, 950, 2074
BseMI	GCAATG	2 cut(s)	1958, 2115
BseMII	CTCAG	9 cut(s)	260, 339, 398, 792, 1229, 1239, 1359, 1497, 1851
BseNI	ACTGG	2 cut(s)	92, 670
BseRI	GAGGAG	5 cut(s)	176, 365, 912, 915, 1261
BseXI	GCAGC	6 cut(s)	26, 799, 1439, 1905, 1933, 1995
Bsh1236I	CGCG	3 cut(s)	823, 1449, 1828
BshFI	GGCC	6 cut(s)	738, 816, 955, 1168, 1462, 1564
BshNI	GGYRCC	2 cut(s)	168, 1243
BshVI	ATCGAT	1 cut(s)	1765
BsiHKAI	GWGCWC	7 cut(s)	360, 811, 1216, 1606, 1627, 2115, 2287
BsiSI	CCGG	5 cut(s)	122, 227, 1094, 1349, 2068
BslFI	GGGAC	3 cut(s)	500, 1894, 2072
BslI	CCNNNNNNNGG	5 cut(s)	220, 280, 369, 950, 2074
BsmAI	GTCTC	3 cut(s)	978, 2190, 2226
BsmFI	GGGAC	3 cut(s)	500, 1894, 2072
BsmI	GAATGC	1 cut(s)	1321
BsnI	GGCC	6 cut(s)	738, 816, 955, 1168, 1462, 1564
Bso31I	GGTCTC	1 cut(s)	978
Bsp1286I	GDGCHC	8 cut(s)	360, 811, 1216, 1606, 1627, 1723, 2115, 2287
Bsp1407I	TGTACA	1 cut(s)	773
Bsp143I	GATC	9 cut(s)	562, 631, 940, 1039, 1444, 1528, 1762, 1840, 2248
Bsp1720I	GCTNAGC	2 cut(s)	1368, 1506
Bsp19I	CCATGG	2 cut(s)	363, 1565
BspACI	CCGC	5 cut(s)	478, 838, 1449, 1592, 1828
BspANI	GGCC	6 cut(s)	738, 816, 955, 1168, 1462, 1564
BspCNI	CTCAG	9 cut(s)	261, 340, 399, 793, 1228, 1240, 1360, 1498, 1850
BspDI	ATCGAT	1 cut(s)	1765
BspFNI	CGCG	3 cut(s)	823, 1449, 1828
BspHI	TCATGA	1 cut(s)	1606
BspLI	GGNNCC	5 cut(s)	170, 225, 510, 1092, 1245
BspMAI	CTGCAG	1 cut(s)	1923
BspMI	ACCTGC	2 cut(s)	120, 847
BspPI	GGATC	4 cut(s)	626, 935, 1047, 1770
BspT107I	GGYRCC	2 cut(s)	168, 1243
BspTNI	GGTCTC	1 cut(s)	978
BsrDI	GCAATG	2 cut(s)	1958, 2115
BsrFI	RCCGGY	2 cut(s)	1093, 2067
BsrGI	TGTACA	1 cut(s)	773
BsrI	ACTGG	2 cut(s)	92, 670
BssAI	RCCGGY	2 cut(s)	1093, 2067
BssECI	CCNNGG	5 cut(s)	26, 296, 363, 1565, 1877
BssMI	GATC	9 cut(s)	562, 631, 940, 1039, 1444, 1528, 1762, 1840, 2248
BssNI	GRCGYC	1 cut(s)	1726
BssSI	CACGAG	1 cut(s)	810
BssT1I	CCWWGG	3 cut(s)	296, 363, 1565
Bst2BI	CACGAG	1 cut(s)	810
Bst2UI	CCWGG	3 cut(s)	28, 399, 1878
Bst4CI	ACNGT	5 cut(s)	148, 173, 652, 725, 1928
Bst6I	CTCTTC	1 cut(s)	2193
BstACI	GRCGYC	1 cut(s)	1726
BstAPI	GCANNNNNTGC	1 cut(s)	1487
BstAUI	TGTACA	1 cut(s)	773
BstBAI	YACGTR	2 cut(s)	656, 1716
BstC8I	GCNNGC	3 cut(s)	211, 360, 1492
BstDSI	CCRYGG	2 cut(s)	363, 1565
BstENI	CCTNNNNNAGG	1 cut(s)	948
BstF5I	GGATG	5 cut(s)	10, 440, 943, 1240, 2143
BstFNI	CGCG	3 cut(s)	823, 1449, 1828
BstH2I	RGCGCY	1 cut(s)	703
BstHHI	GCGC	2 cut(s)	702, 1828
BstKTI	GATC	9 cut(s)	565, 634, 943, 1042, 1447, 1531, 1765, 1843, 2251
BstMAI	GTCTC	3 cut(s)	978, 2190, 2226
BstMBI	GATC	9 cut(s)	562, 631, 940, 1039, 1444, 1528, 1762, 1840, 2248
BstMWI	GCNNNNNNNGC	4 cut(s)	1487, 2005, 2017, 2033
BstNI	CCWGG	3 cut(s)	28, 399, 1878
BstNSI	RCATGY	1 cut(s)	595
BstSCI	CCNGG	4 cut(s)	26, 226, 397, 1876
BstSFI	CTRYAG	1 cut(s)	1919
BstSNI	TACGTA	1 cut(s)	656
BstUI	CGCG	3 cut(s)	823, 1449, 1828
BstV1I	GCAGC	6 cut(s)	26, 799, 1439, 1905, 1933, 1995
BstX2I	RGATCY	1 cut(s)	1039
BstYI	RGATCY	1 cut(s)	1039
Bsu15I	ATCGAT	1 cut(s)	1765
BsuI	GTATCC	1 cut(s)	40
BsuRI	GGCC	6 cut(s)	738, 816, 955, 1168, 1462, 1564
BsuTUI	ATCGAT	1 cut(s)	1765
BtgI	CCRYGG	2 cut(s)	363, 1565
BtsCI	GGATG	5 cut(s)	10, 440, 943, 1240, 2143
BtsI	GCAGTG	1 cut(s)	425
BtsIMutI	CAGTG	5 cut(s)	99, 153, 425, 721, 1933
BveI	ACCTGC	2 cut(s)	120, 847
Cac8I	GCNNGC	3 cut(s)	211, 360, 1492
CciI	TCATGA	1 cut(s)	1606
CfoI	GCGC	2 cut(s)	702, 1828
Cfr10I	RCCGGY	2 cut(s)	1093, 2067
Cfr13I	GGNCC	5 cut(s)	293, 736, 953, 1562, 1959
ClaI	ATCGAT	1 cut(s)	1765
CseI	GACGC	3 cut(s)	1499, 1715, 2087
Csp6I	GTAC	8 cut(s)	169, 653, 694, 774, 1023, 1244, 1437, 1818
CviQI	GTAC	8 cut(s)	169, 653, 694, 774, 1023, 1244, 1437, 1818
DpnI	GATC	9 cut(s)	564, 633, 942, 1041, 1446, 1530, 1764, 1842, 2250
DpnII	GATC	9 cut(s)	562, 631, 940, 1039, 1444, 1528, 1762, 1840, 2248
EaeI	YGGCCR	1 cut(s)	814
Eam1104I	CTCTTC	1 cut(s)	2193
EarI	CTCTTC	1 cut(s)	2193
Ecl136II	GAGCTC	2 cut(s)	809, 1604
Eco105I	TACGTA	1 cut(s)	656
Eco130I	CCWWGG	3 cut(s)	296, 363, 1565
Eco147I	AGGCCT	2 cut(s)	1168, 1462
Eco24I	GRGCYC	3 cut(s)	811, 1606, 1723
Eco31I	GGTCTC	1 cut(s)	978
Eco47I	GGWCC	2 cut(s)	293, 1959
Eco53kI	GAGCTC	2 cut(s)	809, 1604
Eco57I	CTGAAG	1 cut(s)	1557
EcoICRI	GAGCTC	2 cut(s)	809, 1604
EcoNI	CCTNNNNNAGG	1 cut(s)	948
EcoO109I	RGGNCCY	1 cut(s)	293
EcoRI	GAATTC	2 cut(s)	402, 1252
EcoRII	CCWGG	3 cut(s)	26, 397, 1876
EcoT14I	CCWWGG	3 cut(s)	296, 363, 1565
EcoT38I	GRGCYC	3 cut(s)	811, 1606, 1723
ErhI	CCWWGG	3 cut(s)	296, 363, 1565
FalI	AAGNNNNNCTT	2 cut(s)	1044, 1076
FaqI	GGGAC	3 cut(s)	500, 1894, 2072
FauNDI	CATATG	1 cut(s)	198
FbaI	TGATCA	1 cut(s)	562
FblI	GTMKAC	1 cut(s)	1735
Fnu4HI	GCNGC	8 cut(s)	15, 479, 788, 838, 1428, 1919, 1922, 2009
FokI	GGATG	5 cut(s)	17, 427, 950, 1247, 2150
FriOI	GRGCYC	3 cut(s)	811, 1606, 1723
Fsp4HI	GCNGC	8 cut(s)	15, 479, 788, 838, 1428, 1919, 1922, 2009
FspBI	CTAG	6 cut(s)	600, 945, 1140, 1197, 1458, 2127
GlaI	GCGC	2 cut(s)	701, 1827
GluI	GCNGC	8 cut(s)	15, 479, 788, 838, 1428, 1919, 1922, 2009
HaeII	RGCGCY	1 cut(s)	703
HaeIII	GGCC	6 cut(s)	738, 816, 955, 1168, 1462, 1564
HapII	CCGG	5 cut(s)	122, 227, 1094, 1349, 2068
HgaI	GACGC	3 cut(s)	1499, 1715, 2087
HhaI	GCGC	2 cut(s)	702, 1828
Hin1I	GRCGYC	1 cut(s)	1726
Hin6I	GCGC	2 cut(s)	700, 1826
HinP1I	GCGC	2 cut(s)	700, 1826
HinfI	GANTC	3 cut(s)	424, 1193, 2183
HpaII	CCGG	5 cut(s)	122, 227, 1094, 1349, 2068
HphI	GGTGA	4 cut(s)	1174, 1592, 2057, 2258
Hpy166II	GTNNAC	7 cut(s)	106, 194, 423, 1342, 1713, 1736, 2077
Hpy188III	TCNNGA	2 cut(s)	1607, 1798
Hpy8I	GTNNAC	7 cut(s)	106, 194, 423, 1342, 1713, 1736, 2077
Hpy99I	CGWCG	5 cut(s)	837, 846, 1515, 1641, 1731
HpyAV	CCTTC	9 cut(s)	4, 128, 224, 266, 343, 944, 989, 1080, 1153
HpyCH4III	ACNGT	5 cut(s)	148, 173, 652, 725, 1928
HpyCH4IV	ACGT	4 cut(s)	655, 844, 1715, 2230
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1487, 2005, 2017, 2033
HpySE526I	ACGT	4 cut(s)	655, 844, 1715, 2230
Hsp92I	GRCGYC	1 cut(s)	1726
HspAI	GCGC	2 cut(s)	700, 1826
KpnI	GGTACC	2 cut(s)	172, 1247
Ksp22I	TGATCA	1 cut(s)	562
Kzo9I	GATC	9 cut(s)	562, 631, 940, 1039, 1444, 1528, 1762, 1840, 2248
Lsp1109I	GCAGC	6 cut(s)	26, 799, 1439, 1905, 1933, 1995
MaeI	CTAG	6 cut(s)	600, 945, 1140, 1197, 1458, 2127
MaeII	ACGT	4 cut(s)	655, 844, 1715, 2230
MaeIII	GTNAC	4 cut(s)	148, 762, 1031, 2226
MalI	GATC	9 cut(s)	564, 633, 942, 1041, 1446, 1530, 1764, 1842, 2250
MboI	GATC	9 cut(s)	562, 631, 940, 1039, 1444, 1528, 1762, 1840, 2248
MboII	GAAGA	6 cut(s)	741, 893, 1202, 1527, 1624, 2180
MflI	RGATCY	1 cut(s)	1039
MhlI	GDGCHC	8 cut(s)	360, 811, 1216, 1606, 1627, 1723, 2115, 2287
MlyI	GAGTC	2 cut(s)	418, 2192
MmeI	TCCRAC	3 cut(s)	468, 1740, 2115
MroXI	GAANNNNTTC	1 cut(s)	1683
MslI	CAYNNNNRTG	5 cut(s)	311, 891, 1499, 2070, 2127
MspI	CCGG	5 cut(s)	122, 227, 1094, 1349, 2068
MspR9I	CCNGG	4 cut(s)	28, 228, 399, 1878
Mva1269I	GAATGC	1 cut(s)	1321
MvaI	CCWGG	3 cut(s)	28, 399, 1878
MvnI	CGCG	3 cut(s)	823, 1449, 1828
MwoI	GCNNNNNNNGC	4 cut(s)	1487, 2005, 2017, 2033
NciI	CCSGG	1 cut(s)	228
NcoI	CCATGG	2 cut(s)	363, 1565
NdeI	CATATG	1 cut(s)	198
NdeII	GATC	9 cut(s)	562, 631, 940, 1039, 1444, 1528, 1762, 1840, 2248
NlaIV	GGNNCC	5 cut(s)	170, 225, 510, 1092, 1245
NmeAIII	GCCGAG	1 cut(s)	1702
NmuCI	GTSAC	1 cut(s)	148
NspI	RCATGY	1 cut(s)	595
OliI	CACNNNNGTG	1 cut(s)	2070
PagI	TCATGA	1 cut(s)	1606
PceI	AGGCCT	2 cut(s)	1168, 1462
PcsI	WCGNNNNNNNCGW	2 cut(s)	841, 1762
PctI	GAATGC	1 cut(s)	1321
PdmI	GAANNNNTTC	1 cut(s)	1683
PfeI	GAWTC	1 cut(s)	1193
PflMI	CCANNNNNTGG	1 cut(s)	369
PfoI	TCCNGGA	1 cut(s)	226
PkrI	GCNGC	8 cut(s)	16, 480, 789, 839, 1429, 1920, 1923, 2010
PleI	GAGTC	2 cut(s)	418, 2191
PpsI	GAGTC	2 cut(s)	418, 2191
Ppu21I	YACGTR	2 cut(s)	656, 1716
PpuMI	RGGWCCY	1 cut(s)	293
Psp124BI	GAGCTC	2 cut(s)	811, 1606
Psp5II	RGGWCCY	1 cut(s)	293
Psp6I	CCWGG	3 cut(s)	26, 397, 1876
PspGI	CCWGG	3 cut(s)	26, 397, 1876
PspN4I	GGNNCC	5 cut(s)	170, 225, 510, 1092, 1245
PspPI	GGNCC	5 cut(s)	293, 736, 953, 1562, 1959
PspPPI	RGGWCCY	1 cut(s)	293
PstI	CTGCAG	1 cut(s)	1923
PsuI	RGATCY	1 cut(s)	1039
RsaI	GTAC	8 cut(s)	170, 654, 695, 775, 1024, 1245, 1438, 1819
RsaNI	GTAC	8 cut(s)	169, 653, 694, 774, 1023, 1244, 1437, 1818
RseI	CAYNNNNRTG	5 cut(s)	311, 891, 1499, 2070, 2127
SacI	GAGCTC	2 cut(s)	811, 1606
SatI	GCNGC	8 cut(s)	15, 479, 788, 838, 1428, 1919, 1922, 2009
Sau3AI	GATC	9 cut(s)	562, 631, 940, 1039, 1444, 1528, 1762, 1840, 2248
Sau96I	GGNCC	5 cut(s)	293, 736, 953, 1562, 1959
SchI	GAGTC	2 cut(s)	418, 2192
ScrFI	CCNGG	4 cut(s)	28, 228, 399, 1878
SduI	GDGCHC	8 cut(s)	360, 811, 1216, 1606, 1627, 1723, 2115, 2287
SfcI	CTRYAG	1 cut(s)	1919
SgrAI	CRCCGGYG	1 cut(s)	2067
SinI	GGWCC	2 cut(s)	293, 1959
SmiMI	CAYNNNNRTG	5 cut(s)	311, 891, 1499, 2070, 2127
SmlI	CTYRAG	2 cut(s)	465, 791
SmoI	CTYRAG	2 cut(s)	465, 791
SnaBI	TACGTA	1 cut(s)	656
SseBI	AGGCCT	2 cut(s)	1168, 1462
SsiI	CCGC	5 cut(s)	478, 838, 1449, 1592, 1828
SspMI	CTAG	6 cut(s)	600, 945, 1140, 1197, 1458, 2127
SstI	GAGCTC	2 cut(s)	811, 1606
StuI	AGGCCT	2 cut(s)	1168, 1462
StyD4I	CCNGG	4 cut(s)	26, 226, 397, 1876
StyI	CCWWGG	3 cut(s)	296, 363, 1565
TaaI	ACNGT	5 cut(s)	148, 173, 652, 725, 1928
TaiI	ACGT	4 cut(s)	658, 847, 1718, 2233
TaqI	TCGA	3 cut(s)	1513, 1765, 2281
TatI	WGTACW	3 cut(s)	773, 1436, 1817
TauI	GCSGC	2 cut(s)	481, 840
TfiI	GAWTC	1 cut(s)	1193
TscAI	CASTG	5 cut(s)	99, 153, 425, 728, 1933
TseFI	GTSAC	1 cut(s)	148
TseI	GCWGC	6 cut(s)	14, 787, 1427, 1918, 1921, 2008
Tsp45I	GTSAC	1 cut(s)	148
TspDTI	ATGAA	9 cut(s)	147, 206, 248, 295, 741, 1251, 1676, 2175, 2258
TspRI	CASTG	5 cut(s)	99, 153, 425, 728, 1933
Van91I	CCANNNNNTGG	1 cut(s)	369
VpaK11BI	GGWCC	2 cut(s)	293, 1959
XagI	CCTNNNNNAGG	1 cut(s)	948
XapI	RAATTY	8 cut(s)	402, 500, 778, 1120, 1252, 1652, 1940, 2144
XceI	RCATGY	1 cut(s)	595
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1404
XmiI	GTMKAC	1 cut(s)	1735
XmnI	GAANNNNTTC	1 cut(s)	1683
XspI	CTAG	6 cut(s)	600, 945, 1140, 1197, 1458, 2127

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.