Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0016931

Physical Location & Seq

Genomic Coordinates Forward (+)

1062065 .. 1063007

Gene Structure 943 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1009300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 891 bp

ATGAAAGTCTGCTCCACAACCTTGAAAAGGATATGCAGACAACATGGGATTAAGCGTTGGCCATCTCGAACAATCAAGAAGGTTGGTCACTCTTTACAGAAACTCCAACTTGTAATCGACTCAGTTGAAGGCGCATCAGGTGCTTTCCAGATCAACTCTTTCTATACGAACTTCCCAGAGCTAACATCTCCAAATTTATCGGGGACCAGCCCATTTTCAAGTTCAAAGCTGAGTGATCAACCAAATCAGACAAACTTGTCACCCAAAGGCGGTGTTTTGAGCCCCCAAGCTACCGCATCAAAATCTCCCCCATCTTCATGCTGTCAAAGTTCCAGTTCAAGCCAAAGCTGTTCCAGCAGGACACAGCAACTTCCCTTCTCATGTAACATTGCTGGTAACGATGATCCAGTTGTTGGAGATAACTCAAGAGATGATGTGCTAAAGAGGGTCAGAAGTGAGGCAGGATTGCATGCTTTTGGCCAAGATCGAGCGGAGCTACTGCCAAGATCCCAAAGCCAGAAACTTCTCTGCGAGCAACAAAATCTGCAGTCTATTCCGCAGTCACTGAAAAATAATGGCCGGGTAGCTCAAGAAGGCAAGGTTCAAAGAGTAAAAGTTGCATATGGGGATGAGAAAACCCGCTTCCGCATGCAGAGTAATTGGAGACATGAAGATTTGGTGCAAGAAATCGCAAAGCGATTTAGCATACAAGACATGAGTACATATGATATAAAATACTTGGATGATGACTCGGAGTGGGTCTTGTTAACATGTGATGATGATTTAGAGGAGTGTGTTGATGTGTGCCGGTCTTCAGAGAGCCGCACAATTAAGCTCTCTCTCCAGATATCTCGTCATCACTTGAAAGGTTTGATAGGTAGACCGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PB1 RWP-RK

Protein Analysis

297

Amino Acids

32.93

Weight (kDa)

8.89

Isoelectric Point (pI)

71.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RWP-RK	PF02042	1 - 28	2.4e-11	RWP-RK domain
PB1	PF00564	203 - 282	6.4e-21	PB1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016931)

Species	Orthologous Gene IDs
malus_domestica	MD06G1009300.v1.1
rosa_chinensis	RchiOBHm_Chr6g0280731 RchiOBHm_Chr6g0280741
rosa_laevigata	RLG00000013031
rosa_multiflora	Rmu_co8197134.1_g000001 Rmu_sc0001197.1_g000011 Rmu_sc0025553.1_g000001 Rmu_sc0025867.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187710
rosa_samantha	Rh6AG246800 Rh6AG246900 Rh6BG249200 Rh6CG250300
rosa_wichuraiana	Rw6G021380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	256
AccB7I	CCANNNNNTGG	1 cut(s)	413
AccBSI	CCGCTC	1 cut(s)	491
AccI	GTMKAC	1 cut(s)	880
AciI	CCGC	7 cut(s)	270, 294, 491, 557, 640, 646, 823
AclWI	GGATC	2 cut(s)	398, 501
AcoI	YGGCCR	3 cut(s)	59, 478, 577
AcsI	RAATTY	1 cut(s)	193
AcuI	CTGAAG	1 cut(s)	798
AfaI	GTAC	1 cut(s)	721
AfiI	CCNNNNNNNGG	3 cut(s)	27, 269, 413
AflIII	ACRYGT	1 cut(s)	770
AgsI	TTSAA	7 cut(s)	25, 128, 219, 225, 339, 605, 865
AluBI	AGCT	7 cut(s)	181, 229, 290, 348, 496, 587, 835
AluI	AGCT	7 cut(s)	181, 229, 290, 348, 496, 587, 835
Alw26I	GTCTC	1 cut(s)	658
AlwI	GGATC	2 cut(s)	398, 501
AlwNI	CAGNNNCTG	1 cut(s)	565
AoxI	GGCC	3 cut(s)	59, 478, 577
ApoI	RAATTY	1 cut(s)	193
AspLEI	GCGC	1 cut(s)	134
AspS9I	GGNCC	1 cut(s)	204
AsuC2I	CCSGG	1 cut(s)	581
AsuHPI	GGTGA	1 cut(s)	252
AvaII	GGWCC	1 cut(s)	204
BalI	TGGCCA	2 cut(s)	61, 480
BanII	GRGCYC	1 cut(s)	284
BbsI	GAAGAC	1 cut(s)	804
BccI	CCATC	2 cut(s)	70, 319
BclI	TGATCA	1 cut(s)	235
BcnI	CCSGG	1 cut(s)	581
BcoDI	GTCTC	1 cut(s)	658
BfmI	CTRYAG	1 cut(s)	545
BisI	GCNGC	1 cut(s)	823
BlsI	GCNGC	1 cut(s)	824
Bme1390I	CCNGG	1 cut(s)	581
Bme18I	GGWCC	1 cut(s)	204
BmgT120I	GGNCC	1 cut(s)	204
BmiI	GGNNCC	1 cut(s)	205
BmrFI	CCNGG	1 cut(s)	581
BmsI	GCATC	2 cut(s)	143, 305
BpiI	GAAGAC	1 cut(s)	804
BpmI	CTGGAG	1 cut(s)	827
BpuEI	CTTGAG	2 cut(s)	409, 573
BpuMI	CCSGG	1 cut(s)	581
BsaXI	ACNNNNNCTCC	2 cut(s)	87, 117
Bsc4I	CCNNNNNNNGG	3 cut(s)	27, 269, 413
Bse118I	RCCGGY	1 cut(s)	807
Bse1I	ACTGG	2 cut(s)	333, 407
Bse3DI	GCAATG	1 cut(s)	387
BseGI	GGATG	2 cut(s)	634, 748
BseLI	CCNNNNNNNGG	3 cut(s)	27, 269, 413
BseMI	GCAATG	1 cut(s)	387
BseMII	CTCAG	2 cut(s)	135, 221
BseNI	ACTGG	2 cut(s)	333, 407
BseRI	GAGGAG	1 cut(s)	803
BshFI	GGCC	3 cut(s)	61, 480, 579
BsiSI	CCGG	2 cut(s)	580, 808
BslFI	GGGAC	1 cut(s)	217
BslI	CCNNNNNNNGG	3 cut(s)	27, 269, 413
BsmAI	GTCTC	1 cut(s)	658
BsmFI	GGGAC	1 cut(s)	217
BsnI	GGCC	3 cut(s)	61, 480, 579
Bsp1286I	GDGCHC	1 cut(s)	284
Bsp143I	GATC	5 cut(s)	150, 235, 403, 484, 506
BspACI	CCGC	7 cut(s)	270, 294, 491, 557, 640, 646, 823
BspANI	GGCC	3 cut(s)	61, 480, 579
BspCNI	CTCAG	2 cut(s)	134, 222
BspHI	TCATGA	1 cut(s)	887
BspLI	GGNNCC	1 cut(s)	205
BspMAI	CTGCAG	1 cut(s)	549
BspPI	GGATC	2 cut(s)	398, 501
BsrBI	CCGCTC	1 cut(s)	491
BsrDI	GCAATG	1 cut(s)	387
BsrFI	RCCGGY	1 cut(s)	807
BsrI	ACTGG	2 cut(s)	333, 407
BssAI	RCCGGY	1 cut(s)	807
BssMI	GATC	5 cut(s)	150, 235, 403, 484, 506
Bst4CI	ACNGT	1 cut(s)	885
BstAPI	GCANNNNNTGC	1 cut(s)	140
BstC8I	GCNNGC	3 cut(s)	471, 533, 650
BstDEI	CTNAG	2 cut(s)	121, 230
BstENI	CCTNNNNNAGG	1 cut(s)	25
BstF5I	GGATG	2 cut(s)	634, 748
BstHHI	GCGC	1 cut(s)	134
BstKTI	GATC	5 cut(s)	153, 238, 406, 487, 509
BstMAI	GTCTC	1 cut(s)	658
BstMBI	GATC	5 cut(s)	150, 235, 403, 484, 506
BstMWI	GCNNNNNNNGC	2 cut(s)	140, 354
BstNSI	RCATGY	3 cut(s)	473, 652, 774
BstSCI	CCNGG	1 cut(s)	579
BstSFI	CTRYAG	1 cut(s)	545
BstV2I	GAAGAC	1 cut(s)	804
BstX2I	RGATCY	1 cut(s)	506
BstYI	RGATCY	1 cut(s)	506
BsuRI	GGCC	3 cut(s)	61, 480, 579
BtsCI	GGATG	2 cut(s)	634, 748
BtsIMutI	CAGTG	1 cut(s)	563
Cac8I	GCNNGC	3 cut(s)	471, 533, 650
CaiI	CAGNNNCTG	1 cut(s)	565
CciI	TCATGA	1 cut(s)	887
CfoI	GCGC	1 cut(s)	134
Cfr10I	RCCGGY	1 cut(s)	807
Cfr13I	GGNCC	1 cut(s)	204
Csp6I	GTAC	1 cut(s)	720
CviAII	CATG	9 cut(s)	44, 318, 381, 470, 649, 668, 715, 771, 888
CviQI	GTAC	1 cut(s)	720
DdeI	CTNAG	2 cut(s)	121, 230
DpnI	GATC	5 cut(s)	152, 237, 405, 486, 508
DpnII	GATC	5 cut(s)	150, 235, 403, 484, 506
DrdI	GACNNNNNNGTC	1 cut(s)	256
DseDI	GACNNNNNNGTC	1 cut(s)	256
EaeI	YGGCCR	3 cut(s)	59, 478, 577
Eco24I	GRGCYC	1 cut(s)	284
Eco32I	GATATC	1 cut(s)	849
Eco47I	GGWCC	1 cut(s)	204
Eco57I	CTGAAG	1 cut(s)	798
EcoNI	CCTNNNNNAGG	1 cut(s)	25
EcoRV	GATATC	1 cut(s)	849
EcoT38I	GRGCYC	1 cut(s)	284
FaeI	CATG	9 cut(s)	47, 321, 384, 473, 652, 671, 718, 774, 891
FaqI	GGGAC	1 cut(s)	217
FatI	CATG	9 cut(s)	43, 317, 380, 469, 648, 667, 714, 770, 887
FauI	CCCGC	1 cut(s)	647
FauNDI	CATATG	2 cut(s)	622, 724
FbaI	TGATCA	1 cut(s)	235
FblI	GTMKAC	1 cut(s)	880
Fnu4HI	GCNGC	1 cut(s)	823
FokI	GGATG	2 cut(s)	641, 755
FriOI	GRGCYC	1 cut(s)	284
Fsp4HI	GCNGC	1 cut(s)	823
GlaI	GCGC	1 cut(s)	133
GluI	GCNGC	1 cut(s)	823
GsuI	CTGGAG	1 cut(s)	827
HaeIII	GGCC	3 cut(s)	61, 480, 579
HapII	CCGG	2 cut(s)	580, 808
HhaI	GCGC	1 cut(s)	134
Hin1II	CATG	9 cut(s)	47, 321, 384, 473, 652, 671, 718, 774, 891
Hin6I	GCGC	1 cut(s)	132
HinP1I	GCGC	1 cut(s)	132
HincII	GTYRAC	1 cut(s)	768
HindII	GTYRAC	1 cut(s)	768
HinfI	GANTC	2 cut(s)	119, 749
HpaI	GTTAAC	1 cut(s)	768
HpaII	CCGG	2 cut(s)	580, 808
HphI	GGTGA	1 cut(s)	252
Hpy166II	GTNNAC	2 cut(s)	768, 881
Hpy188I	TCNGA	4 cut(s)	249, 452, 754, 817
Hpy188III	TCNNGA	7 cut(s)	66, 76, 148, 426, 590, 844, 888
Hpy8I	GTNNAC	2 cut(s)	768, 881
HpyAV	CCTTC	4 cut(s)	73, 122, 385, 587
HpyCH4III	ACNGT	1 cut(s)	885
HpyCH4V	TGCA	6 cut(s)	36, 469, 547, 620, 652, 682
HpyF10VI	GCNNNNNNNGC	2 cut(s)	140, 354
HpyF3I	CTNAG	2 cut(s)	121, 230
Hsp92II	CATG	9 cut(s)	47, 321, 384, 473, 652, 671, 718, 774, 891
HspAI	GCGC	1 cut(s)	132
Ksp22I	TGATCA	1 cut(s)	235
KspAI	GTTAAC	1 cut(s)	768
Kzo9I	GATC	5 cut(s)	150, 235, 403, 484, 506
LmnI	GCTCC	2 cut(s)	17, 493
LweI	GCATC	2 cut(s)	143, 305
MaeIII	GTNAC	5 cut(s)	86, 258, 383, 395, 561
MalI	GATC	5 cut(s)	152, 237, 405, 486, 508
MbiI	CCGCTC	1 cut(s)	491
MboI	GATC	5 cut(s)	150, 235, 403, 484, 506
MboII	GAAGA	3 cut(s)	306, 683, 804
MflI	RGATCY	1 cut(s)	506
MhlI	GDGCHC	1 cut(s)	284
MlsI	TGGCCA	2 cut(s)	61, 480
MluCI	AATT	3 cut(s)	193, 658, 828
MluNI	TGGCCA	2 cut(s)	61, 480
MlyI	GAGTC	2 cut(s)	113, 743
MmeI	TCCRAC	2 cut(s)	130, 394
MnlI	CCTC	3 cut(s)	438, 451, 781
Mox20I	TGGCCA	2 cut(s)	61, 480
MscI	TGGCCA	2 cut(s)	61, 480
MseI	TTAA	3 cut(s)	51, 767, 831
MslI	CAYNNNNRTG	1 cut(s)	316
Msp20I	TGGCCA	2 cut(s)	61, 480
MspI	CCGG	2 cut(s)	580, 808
MspR9I	CCNGG	1 cut(s)	581
MwoI	GCNNNNNNNGC	2 cut(s)	140, 354
NciI	CCSGG	1 cut(s)	581
NdeI	CATATG	2 cut(s)	622, 724
NdeII	GATC	5 cut(s)	150, 235, 403, 484, 506
NlaIII	CATG	9 cut(s)	47, 321, 384, 473, 652, 671, 718, 774, 891
NlaIV	GGNNCC	1 cut(s)	205
NmuCI	GTSAC	3 cut(s)	86, 258, 561
NspI	RCATGY	3 cut(s)	473, 652, 774
PaeI	GCATGC	2 cut(s)	473, 652
PagI	TCATGA	1 cut(s)	887
PciI	ACATGT	1 cut(s)	770
PflMI	CCANNNNNTGG	1 cut(s)	413
PkrI	GCNGC	1 cut(s)	824
PleI	GAGTC	2 cut(s)	113, 743
PpsI	GAGTC	2 cut(s)	113, 743
PscI	ACATGT	1 cut(s)	770
PspN4I	GGNNCC	1 cut(s)	205
PspPI	GGNCC	1 cut(s)	204
PstI	CTGCAG	1 cut(s)	549
PstNI	CAGNNNCTG	1 cut(s)	565
PsuI	RGATCY	1 cut(s)	506
RsaI	GTAC	1 cut(s)	721
RsaNI	GTAC	1 cut(s)	720
RseI	CAYNNNNRTG	1 cut(s)	316
SaqAI	TTAA	3 cut(s)	51, 767, 831
SatI	GCNGC	1 cut(s)	823
Sau3AI	GATC	5 cut(s)	150, 235, 403, 484, 506
Sau96I	GGNCC	1 cut(s)	204
SchI	GAGTC	2 cut(s)	113, 743
ScrFI	CCNGG	1 cut(s)	581
SduI	GDGCHC	1 cut(s)	284
SfaNI	GCATC	2 cut(s)	143, 305
SfcI	CTRYAG	1 cut(s)	545
SinI	GGWCC	1 cut(s)	204
SmiMI	CAYNNNNRTG	1 cut(s)	316
SmlI	CTYRAG	2 cut(s)	424, 588
SmoI	CTYRAG	2 cut(s)	424, 588
SphI	GCATGC	2 cut(s)	473, 652
Sse9I	AATT	3 cut(s)	193, 658, 828
SsiI	CCGC	7 cut(s)	270, 294, 491, 557, 640, 646, 823
StyD4I	CCNGG	1 cut(s)	579
TaaI	ACNGT	1 cut(s)	885
TaqI	TCGA	3 cut(s)	67, 117, 487
TasI	AATT	3 cut(s)	193, 658, 828
TatI	WGTACW	1 cut(s)	719
TauI	GCSGC	1 cut(s)	825
Tru1I	TTAA	3 cut(s)	51, 767, 831
Tru9I	TTAA	3 cut(s)	51, 767, 831
TscAI	CASTG	1 cut(s)	570
TseFI	GTSAC	3 cut(s)	86, 258, 561
Tsp45I	GTSAC	3 cut(s)	86, 258, 561
TspDTI	ATGAA	3 cut(s)	17, 306, 684
TspRI	CASTG	1 cut(s)	570
Van91I	CCANNNNNTGG	1 cut(s)	413
VpaK11BI	GGWCC	1 cut(s)	204
XagI	CCTNNNNNAGG	1 cut(s)	25
XapI	RAATTY	1 cut(s)	193
XceI	RCATGY	3 cut(s)	473, 652, 774
XmiI	GTMKAC	1 cut(s)	880

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.