Rh6AG246800

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0016931
Physical Location & Seq
Forward (+)
43541279 .. 43541899
621 bp
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UTR
Exon/CDS
Intron
Rh6AG246800.1

Sequence Viewer

Length: 456 bp
ATGTTTGGCCTGCATGCTTCTGTGGCAATTCCCTTTCGAAGTGTCTATACTGGATCAGCTGATTTTGTTCTAGAGTTTTTCTTACCCAAGGATTGCCCAGATCCTGAAAAACAGAAGCAGATGCTCAACTCATTCTGCATTGTTACACAACAGGCTTGCCGGAGGTTACATGTTGATGTAGACAAAAAACTCGAAGAGGAACTCAGAAGACCAAAGGAAGAGTTCAAGGTCACAAGCCATTGGGATAACACAGGCAGTTTACAGTACACAGTGGGCAGTGGGCAGGTGTTTTCAGATTTTGGGAGACTTCATCAAAGTTCTGGATCACATGGTCTTGTTGAGGGTGGTATAGACTCTTATTCTTTTGGCGGACGTAGGGCATCAGGAGGCAGAAAAGCTGGTGAGAAGAGGAGGACAAAGACTGAGAAGACGATCAGCTTGCCAGTTCTTAGGTAA

Protein Analysis

151

Amino Acids

16.92

Weight (kDa)

9.24

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAF_NLP PF22922 1 - 56 8.8e-19 NLP protein GAF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 274
Acc36I ACCTGC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 180
AciI CCGC 1 cut(s) 369
AclWI GGATC 3 cut(s) 61, 95, 331
AfaI GTAC 1 cut(s) 266
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 1 cut(s) 226
AluBI AGCT 3 cut(s) 59, 398, 438
AluI AGCT 3 cut(s) 59, 398, 438
Alw26I GTCTC 1 cut(s) 298
AlwI GGATC 3 cut(s) 61, 95, 331
AlwNI CAGNNNCTG 1 cut(s) 104
AoxI GGCC 1 cut(s) 7
AsuHPI GGTGA 1 cut(s) 413
AsuII TTCGAA 1 cut(s) 37
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
BbsI GAAGAC 2 cut(s) 214, 434
BcgI CGANNNNNNTGC 2 cut(s) 421, 455
BcoDI GTCTC 1 cut(s) 298
BfaI CTAG 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 274
BmsI GCATC 2 cut(s) 111, 389
BpiI GAAGAC 2 cut(s) 214, 434
Bpu14I TTCGAA 1 cut(s) 37
BsaJI CCNNGG 1 cut(s) 87
Bse1I ACTGG 2 cut(s) 55, 443
BseDI CCNNGG 1 cut(s) 87
BseMII CTCAG 2 cut(s) 217, 414
BseNI ACTGG 2 cut(s) 55, 443
BseRI GAGGAG 1 cut(s) 424
BshFI GGCC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 298
BsnI GGCC 1 cut(s) 9
Bsp119I TTCGAA 1 cut(s) 37
Bsp143I GATC 4 cut(s) 53, 100, 323, 432
BspACI CCGC 1 cut(s) 369
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 2 cut(s) 216, 415
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 3 cut(s) 61, 95, 331
BspT104I TTCGAA 1 cut(s) 37
BsrI ACTGG 2 cut(s) 55, 443
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 4 cut(s) 53, 100, 323, 432
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 264, 271
Bst6I CTCTTC 3 cut(s) 189, 213, 401
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 4 cut(s) 11, 15, 157, 440
BstDEI CTNAG 3 cut(s) 203, 423, 449
BstKTI GATC 4 cut(s) 56, 103, 326, 435
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 4 cut(s) 53, 100, 323, 432
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNSI RCATGY 2 cut(s) 17, 173
BstV2I GAAGAC 2 cut(s) 214, 434
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 1 cut(s) 9
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 276, 283
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 4 cut(s) 11, 15, 157, 440
CaiI CAGNNNCTG 1 cut(s) 104
Csp6I GTAC 1 cut(s) 265
CviAII CATG 3 cut(s) 14, 170, 329
CviJI RGCY 6 cut(s) 9, 59, 155, 237, 398, 438
CviKI_1 RGCY 6 cut(s) 9, 59, 155, 237, 398, 438
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 3 cut(s) 203, 423, 449
DpnI GATC 4 cut(s) 55, 102, 325, 434
DpnII GATC 4 cut(s) 53, 100, 323, 432
Eam1104I CTCTTC 3 cut(s) 189, 213, 401
EarI CTCTTC 3 cut(s) 189, 213, 401
EciI GGCGGA 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 3 cut(s) 17, 173, 332
FaiI YATR 5 cut(s) 15, 48, 171, 330, 350
FatI CATG 3 cut(s) 13, 169, 328
FblI GTMKAC 1 cut(s) 180
FspBI CTAG 1 cut(s) 71
HaeIII GGCC 1 cut(s) 9
HapII CCGG 1 cut(s) 160
Hin1II CATG 3 cut(s) 17, 173, 332
HinfI GANTC 1 cut(s) 353
HpaII CCGG 1 cut(s) 160
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 3 cut(s) 181, 260, 267
Hpy188I TCNGA 2 cut(s) 206, 295
Hpy188III TCNNGA 4 cut(s) 71, 104, 321, 384
Hpy8I GTNNAC 3 cut(s) 181, 260, 267
HpyCH4III ACNGT 2 cut(s) 264, 271
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 2 cut(s) 13, 138
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 3 cut(s) 203, 423, 449
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 3 cut(s) 17, 173, 332
Kzo9I GATC 4 cut(s) 53, 100, 323, 432
LweI GCATC 2 cut(s) 111, 389
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 3 cut(s) 142, 165, 229
MalI GATC 4 cut(s) 55, 102, 325, 434
MboI GATC 4 cut(s) 53, 100, 323, 432
MboII GAAGA 5 cut(s) 206, 219, 230, 418, 439
MflI RGATCY 1 cut(s) 100
MluCI AATT 1 cut(s) 27
MlyI GAGTC 1 cut(s) 347
MnlI CCTC 6 cut(s) 156, 190, 334, 380, 402, 405
MslI CAYNNNNRTG 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 1 cut(s) 160
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 4 cut(s) 53, 100, 323, 432
NlaIII CATG 3 cut(s) 17, 173, 332
NmuCI GTSAC 1 cut(s) 229
NspI RCATGY 2 cut(s) 17, 173
NspV TTCGAA 1 cut(s) 37
PaeI GCATGC 1 cut(s) 17
PaqCI CACCTGC 1 cut(s) 274
PciI ACATGT 1 cut(s) 169
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
PscI ACATGT 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
RseI CAYNNNNRTG 1 cut(s) 174
Sau3AI GATC 4 cut(s) 53, 100, 323, 432
SchI GAGTC 1 cut(s) 347
SetI ASST 8 cut(s) 61, 167, 231, 288, 376, 400, 440, 455
SfaNI GCATC 2 cut(s) 111, 389
SfuI TTCGAA 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 174
SphI GCATGC 1 cut(s) 17
Sse9I AATT 1 cut(s) 27
SsiI CCGC 1 cut(s) 369
SspMI CTAG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 2 cut(s) 264, 271
TaiI ACGT 1 cut(s) 376
TaqI TCGA 2 cut(s) 37, 192
TasI AATT 1 cut(s) 27
TatI WGTACW 1 cut(s) 264
TscAI CASTG 2 cut(s) 276, 283
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 299
TspRI CASTG 2 cut(s) 276, 283
XbaI TCTAGA 1 cut(s) 70
XceI RCATGY 2 cut(s) 17, 173
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.