Rh6BG249200

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0016931
Physical Location & Seq
Forward (+)
46084310 .. 46091190
6881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG249200.1

Sequence Viewer

Length: 339 bp
ATGTTTGGCCTGCATGCTTCTGTGGCAATTCCCTTTCGAAGTGTCTATACTGGATCAGCTGATTTTGTTCTAGAGTTTTTCTTACCCAAGGATTGCCCAGATCCTGAAAAACAGAAGCAGATGCTCAACTCATTCTGCATTGTTACACAACAGGCTTGCCGGAGGTTACATGTTGATGTAGACAAAAAACTCGAAGAGGAACTCAGGTTTCCTGTTGTTGCTTCAGATGGGGGTCTTCACAGAGAAGAAACTCAAAAACTGATCTTATCCCACAGAAGAGCCAACTGCAAAGTCCACGACGATTCTTGCTCATATGATCGAGGCCAAACAGAAGGGTAA

Protein Analysis

112

Amino Acids

12.77

Weight (kDa)

6.18

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAF_NLP PF22922 1 - 56 4.4e-19 NLP protein GAF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 180
AclWI GGATC 2 cut(s) 61, 95
AcuI CTGAAG 1 cut(s) 207
AflIII ACRYGT 1 cut(s) 169
AluBI AGCT 1 cut(s) 59
AluI AGCT 1 cut(s) 59
AlwI GGATC 2 cut(s) 61, 95
AlwNI CAGNNNCTG 1 cut(s) 104
AoxI GGCC 2 cut(s) 7, 322
AsuII TTCGAA 1 cut(s) 37
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
BbsI GAAGAC 1 cut(s) 227
BccI CCATC 1 cut(s) 221
BfaI CTAG 1 cut(s) 71
BmsI GCATC 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 227
Bpu14I TTCGAA 1 cut(s) 37
BsaJI CCNNGG 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 55
BseDI CCNNGG 1 cut(s) 87
BseMII CTCAG 1 cut(s) 217
BseNI ACTGG 1 cut(s) 55
BshFI GGCC 2 cut(s) 9, 324
BsiSI CCGG 1 cut(s) 160
BsnI GGCC 2 cut(s) 9, 324
Bsp119I TTCGAA 1 cut(s) 37
Bsp143I GATC 4 cut(s) 53, 100, 261, 316
BspANI GGCC 2 cut(s) 9, 324
BspCNI CTCAG 1 cut(s) 216
BspPI GGATC 2 cut(s) 61, 95
BspQI GCTCTTC 1 cut(s) 271
BspT104I TTCGAA 1 cut(s) 37
BsrI ACTGG 1 cut(s) 55
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 4 cut(s) 53, 100, 261, 316
BssT1I CCWWGG 1 cut(s) 87
Bst6I CTCTTC 2 cut(s) 189, 271
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 3 cut(s) 11, 15, 157
BstDEI CTNAG 1 cut(s) 203
BstKTI GATC 4 cut(s) 56, 103, 264, 319
BstMBI GATC 4 cut(s) 53, 100, 261, 316
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNSI RCATGY 2 cut(s) 17, 173
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 2 cut(s) 9, 324
Cac8I GCNNGC 3 cut(s) 11, 15, 157
CaiI CAGNNNCTG 1 cut(s) 104
CviAII CATG 2 cut(s) 14, 170
CviJI RGCY 5 cut(s) 9, 59, 155, 281, 324
CviKI_1 RGCY 5 cut(s) 9, 59, 155, 281, 324
DdeI CTNAG 1 cut(s) 203
DpnI GATC 4 cut(s) 55, 102, 263, 318
DpnII GATC 4 cut(s) 53, 100, 261, 316
Eam1104I CTCTTC 2 cut(s) 189, 271
EarI CTCTTC 2 cut(s) 189, 271
Eco130I CCWWGG 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 2 cut(s) 17, 173
FaiI YATR 5 cut(s) 15, 48, 171, 313, 315
FatI CATG 2 cut(s) 13, 169
FauNDI CATATG 1 cut(s) 313
FblI GTMKAC 1 cut(s) 180
FspBI CTAG 1 cut(s) 71
HaeIII GGCC 2 cut(s) 9, 324
HapII CCGG 1 cut(s) 160
Hin1II CATG 2 cut(s) 17, 173
HinfI GANTC 1 cut(s) 302
HpaII CCGG 1 cut(s) 160
Hpy166II GTNNAC 2 cut(s) 181, 295
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 2 cut(s) 71, 104
Hpy8I GTNNAC 2 cut(s) 181, 295
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 326
HpyCH4V TGCA 3 cut(s) 13, 138, 288
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 1 cut(s) 203
Hsp92II CATG 2 cut(s) 17, 173
Kzo9I GATC 4 cut(s) 53, 100, 261, 316
LguI GCTCTTC 1 cut(s) 271
LpnPI CCDG 8 cut(s) 23, 36, 111, 117, 137, 173, 190, 225
LweI GCATC 1 cut(s) 111
MaeI CTAG 1 cut(s) 71
MaeIII GTNAC 2 cut(s) 142, 165
MalI GATC 4 cut(s) 55, 102, 263, 318
MboI GATC 4 cut(s) 53, 100, 261, 316
MboII GAAGA 4 cut(s) 206, 227, 257, 288
MflI RGATCY 1 cut(s) 100
MluCI AATT 1 cut(s) 27
MnlI CCTC 3 cut(s) 156, 190, 314
MslI CAYNNNNRTG 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 1 cut(s) 160
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeI CATATG 1 cut(s) 313
NdeII GATC 4 cut(s) 53, 100, 261, 316
NlaIII CATG 2 cut(s) 17, 173
NspI RCATGY 2 cut(s) 17, 173
NspV TTCGAA 1 cut(s) 37
PaeI GCATGC 1 cut(s) 17
PciI ACATGT 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 271
PfeI GAWTC 1 cut(s) 302
PscI ACATGT 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 59
RseI CAYNNNNRTG 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 271
Sau3AI GATC 4 cut(s) 53, 100, 261, 316
SetI ASST 3 cut(s) 61, 167, 209
SfaNI GCATC 1 cut(s) 111
SfuI TTCGAA 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 174
SphI GCATGC 1 cut(s) 17
Sse9I AATT 1 cut(s) 27
SspMI CTAG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 87
TaqI TCGA 3 cut(s) 37, 192, 319
TasI AATT 1 cut(s) 27
TfiI GAWTC 1 cut(s) 302
XbaI TCTAGA 1 cut(s) 70
XceI RCATGY 2 cut(s) 17, 173
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.