Rh6CG250300

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0016931
Physical Location & Seq
Forward (+)
42054090 .. 42054722
633 bp
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UTR
Exon/CDS
Intron
Rh6CG250300.1

Sequence Viewer

Length: 633 bp
ATGTTTGGCCTTCATGCTTCTGTAGCAATTCCCTTTCGAAGTGTCTATACTGGATCAGCTGATTTTGTTCTAGAGTTTTTCTTACCCAAGGATTGCCCAGATCCTGAAAAACAGAAGCAGATGCTGAACTCATTCTGCATTGTTACACAACAGGCTTGCCGGAGGTTACATGTTGATGTAGACAAAAAACTCGAAGAGGAACTCAGGTTTCCTGTTGTTGCTGCAGATGGGGGTCTTCACAGAGAAGAAACTCAAAAACTGATGTCTTATCCCACAGAAGAGCCAACTGCAAAGTCCATGAAGATTCTTGCTCATATGATTGAGGCCAAACAGAAGGGTAAAGGTGTATCGATCTGTCCATCTCCTTGGATTATCGAACAGAAGAACCAAAGGAAGAGTTCAAGGTCACAAGCCACTGGGATAACAACCAAGCAGTTTACAGTACACAGTGGCAGGTGTTTTCAGATTTTGGGAGACTTCATCAAAGTTCTGGATCAGATGGTCTTGTTGAGGATGGTGCGGATTCTTATTCTTTTGGCGCGCTTAGGGCATCAGGAGGCAGAAAAGCTGGTGAGAAGAGGAGGACAAAGACTGAGAAGACGATCATCTTGCCAGTTCATAGGCAATACTTAG

Protein Analysis

210

Amino Acids

23.9

Weight (kDa)

9.69

Isoelectric Point (pI)

59.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAF_NLP PF22922 1 - 56 1.7e-18 NLP protein GAF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 444
AccI GTMKAC 1 cut(s) 180
AccII CGCG 1 cut(s) 541
AciI CCGC 1 cut(s) 520
AclWI GGATC 3 cut(s) 61, 95, 501
AfaI GTAC 1 cut(s) 444
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 1 cut(s) 402
AluBI AGCT 2 cut(s) 59, 568
AluI AGCT 2 cut(s) 59, 568
Alw26I GTCTC 1 cut(s) 468
AlwI GGATC 3 cut(s) 61, 95, 501
AlwNI CAGNNNCTG 2 cut(s) 104, 124
AoxI GGCC 2 cut(s) 7, 324
ApeKI GCWGC 1 cut(s) 221
Asp700I GAANNNNTTC 1 cut(s) 131
AspLEI GCGC 2 cut(s) 541, 543
AsuHPI GGTGA 1 cut(s) 583
AsuII TTCGAA 1 cut(s) 37
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
BbsI GAAGAC 2 cut(s) 227, 604
BbvI GCAGC 1 cut(s) 208
BccI CCATC 4 cut(s) 221, 367, 493, 508
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BcoDI GTCTC 1 cut(s) 468
BfaI CTAG 1 cut(s) 71
BfmI CTRYAG 2 cut(s) 21, 222
BfuAI ACCTGC 1 cut(s) 444
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 2 cut(s) 111, 559
BmuI ACTGGG 1 cut(s) 426
BpiI GAAGAC 2 cut(s) 227, 604
Bpu10I CCTNAGC 1 cut(s) 544
Bpu14I TTCGAA 1 cut(s) 37
Bsa29I ATCGAT 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 87, 365
Bse1I ACTGG 3 cut(s) 55, 421, 613
BseCI ATCGAT 1 cut(s) 350
BseDI CCNNGG 2 cut(s) 87, 365
BseGI GGATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 217, 584
BseNI ACTGG 3 cut(s) 55, 421, 613
BsePI GCGCGC 1 cut(s) 539
BseRI GAGGAG 1 cut(s) 594
BseXI GCAGC 1 cut(s) 208
Bsh1236I CGCG 1 cut(s) 541
BshFI GGCC 2 cut(s) 9, 326
BshVI ATCGAT 1 cut(s) 350
BsiSI CCGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 468
BsnI GGCC 2 cut(s) 9, 326
Bsp119I TTCGAA 1 cut(s) 37
Bsp143I GATC 5 cut(s) 53, 100, 351, 493, 602
BspACI CCGC 1 cut(s) 520
BspANI GGCC 2 cut(s) 9, 326
BspCNI CTCAG 2 cut(s) 216, 585
BspDI ATCGAT 1 cut(s) 350
BspFNI CGCG 1 cut(s) 541
BspMAI CTGCAG 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 444
BspPI GGATC 3 cut(s) 61, 95, 501
BspQI GCTCTTC 1 cut(s) 273
BspT104I TTCGAA 1 cut(s) 37
BsrI ACTGG 3 cut(s) 55, 421, 613
BssECI CCNNGG 2 cut(s) 87, 365
BssHII GCGCGC 1 cut(s) 539
BssMI GATC 5 cut(s) 53, 100, 351, 493, 602
BssT1I CCWWGG 2 cut(s) 87, 365
Bst4CI ACNGT 2 cut(s) 442, 449
Bst6I CTCTTC 4 cut(s) 189, 273, 389, 571
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 157, 541
BstDEI CTNAG 4 cut(s) 203, 544, 593, 630
BstF5I GGATG 1 cut(s) 519
BstFNI CGCG 1 cut(s) 541
BstHHI GCGC 2 cut(s) 541, 543
BstKTI GATC 5 cut(s) 56, 103, 354, 496, 605
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 5 cut(s) 53, 100, 351, 493, 602
BstMWI GCNNNNNNNGC 2 cut(s) 23, 547
BstNSI RCATGY 1 cut(s) 173
BstSFI CTRYAG 2 cut(s) 21, 222
BstUI CGCG 1 cut(s) 541
BstV1I GCAGC 1 cut(s) 208
BstV2I GAAGAC 2 cut(s) 227, 604
BstX2I RGATCY 1 cut(s) 100
BstXI CCANNNNNNTGG 1 cut(s) 366
BstYI RGATCY 1 cut(s) 100
Bsu15I ATCGAT 1 cut(s) 350
BsuRI GGCC 2 cut(s) 9, 326
BsuTUI ATCGAT 1 cut(s) 350
BtsCI GGATG 1 cut(s) 519
BtsIMutI CAGTG 2 cut(s) 414, 454
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 2 cut(s) 157, 541
CaiI CAGNNNCTG 2 cut(s) 104, 124
CfoI GCGC 2 cut(s) 541, 543
ClaI ATCGAT 1 cut(s) 350
Csp6I GTAC 1 cut(s) 443
CviAII CATG 3 cut(s) 14, 170, 298
CviJI RGCY 7 cut(s) 9, 59, 155, 283, 326, 413, 568
CviKI_1 RGCY 7 cut(s) 9, 59, 155, 283, 326, 413, 568
CviQI GTAC 1 cut(s) 443
DdeI CTNAG 4 cut(s) 203, 544, 593, 630
DpnI GATC 5 cut(s) 55, 102, 353, 495, 604
DpnII GATC 5 cut(s) 53, 100, 351, 493, 602
Eam1104I CTCTTC 4 cut(s) 189, 273, 389, 571
EarI CTCTTC 4 cut(s) 189, 273, 389, 571
Eco130I CCWWGG 2 cut(s) 87, 365
EcoT14I CCWWGG 2 cut(s) 87, 365
ErhI CCWWGG 2 cut(s) 87, 365
FaeI CATG 3 cut(s) 17, 173, 301
FaiI YATR 7 cut(s) 15, 48, 171, 299, 315, 317, 620
FatI CATG 3 cut(s) 13, 169, 297
FauNDI CATATG 1 cut(s) 315
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 1 cut(s) 526
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 1 cut(s) 71
GlaI GCGC 2 cut(s) 540, 542
GluI GCNGC 1 cut(s) 222
HaeIII GGCC 2 cut(s) 9, 326
HapII CCGG 1 cut(s) 160
HhaI GCGC 2 cut(s) 541, 543
Hin1II CATG 3 cut(s) 17, 173, 301
Hin6I GCGC 2 cut(s) 539, 541
HinP1I GCGC 2 cut(s) 539, 541
HinfI GANTC 2 cut(s) 304, 523
HpaII CCGG 1 cut(s) 160
HphI GGTGA 1 cut(s) 583
Hpy166II GTNNAC 3 cut(s) 181, 438, 445
Hpy188I TCNGA 2 cut(s) 465, 498
Hpy188III TCNNGA 4 cut(s) 71, 104, 491, 554
Hpy8I GTNNAC 3 cut(s) 181, 438, 445
HpyAV CCTTC 2 cut(s) 20, 328
HpyCH4III ACNGT 2 cut(s) 442, 449
HpyCH4V TGCA 3 cut(s) 138, 224, 290
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 547
HpyF3I CTNAG 4 cut(s) 203, 544, 593, 630
Hsp92II CATG 3 cut(s) 17, 173, 301
HspAI GCGC 2 cut(s) 539, 541
Kzo9I GATC 5 cut(s) 53, 100, 351, 493, 602
LguI GCTCTTC 1 cut(s) 273
Lsp1109I GCAGC 1 cut(s) 208
LweI GCATC 2 cut(s) 111, 559
MaeI CTAG 1 cut(s) 71
MaeIII GTNAC 3 cut(s) 142, 165, 405
MalI GATC 5 cut(s) 55, 102, 353, 495, 604
MboI GATC 5 cut(s) 53, 100, 351, 493, 602
MboII GAAGA 9 cut(s) 206, 227, 257, 290, 313, 394, 406, 588, 609
MflI RGATCY 1 cut(s) 100
MluCI AATT 1 cut(s) 27
MnlI CCTC 7 cut(s) 156, 190, 316, 504, 550, 572, 575
MroXI GAANNNNTTC 1 cut(s) 131
MslI CAYNNNNRTG 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 1 cut(s) 160
MvnI CGCG 1 cut(s) 541
MwoI GCNNNNNNNGC 2 cut(s) 23, 547
NdeI CATATG 1 cut(s) 315
NdeII GATC 5 cut(s) 53, 100, 351, 493, 602
NlaIII CATG 3 cut(s) 17, 173, 301
NmuCI GTSAC 1 cut(s) 405
NspI RCATGY 1 cut(s) 173
NspV TTCGAA 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 444
PauI GCGCGC 1 cut(s) 539
PciI ACATGT 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 131
PfeI GAWTC 2 cut(s) 304, 523
PkrI GCNGC 1 cut(s) 223
PscI ACATGT 1 cut(s) 169
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 2 cut(s) 104, 124
PsuI RGATCY 1 cut(s) 100
PteI GCGCGC 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 1 cut(s) 444
RsaNI GTAC 1 cut(s) 443
RseI CAYNNNNRTG 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 273
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 5 cut(s) 53, 100, 351, 493, 602
SetI ASST 7 cut(s) 61, 167, 209, 346, 407, 458, 570
SfaNI GCATC 2 cut(s) 111, 559
SfcI CTRYAG 2 cut(s) 21, 222
SfuI TTCGAA 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 174
Sse9I AATT 1 cut(s) 27
SsiI CCGC 1 cut(s) 520
SspMI CTAG 1 cut(s) 71
StyI CCWWGG 2 cut(s) 87, 365
TaaI ACNGT 2 cut(s) 442, 449
TaqI TCGA 4 cut(s) 37, 192, 350, 375
TasI AATT 1 cut(s) 27
TatI WGTACW 1 cut(s) 442
TfiI GAWTC 2 cut(s) 304, 523
TscAI CASTG 2 cut(s) 421, 454
TseFI GTSAC 1 cut(s) 405
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 405
TspDTI ATGAA 3 cut(s) 314, 469, 607
TspRI CASTG 2 cut(s) 421, 454
XbaI TCTAGA 1 cut(s) 70
XceI RCATGY 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.