RLG00000013031

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016931
Physical Location & Seq
Reverse (-)
25199367 .. 25200561
1195 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013031

Sequence Viewer

Length: 537 bp
ATGTTTGGCCTTCATGCTTTGGTAGCAATTCCCTTTCTAAGTGTCTATACTGGATCAGCTGATTTTGTTCTAGAGTTTTTCTTACCCAAGGATTGCCCAGATCCTGAAAAACAGAACCAGATGCTGAACTCATTCTGCATTGTTACACAACAGGCTTGCCGGAGGTTACATGTTGATACTGGCGGCATATTCACCCATGGAACCACTACAAAGTCACCATCGTCCTCTTGCAGTCAGACTTCTGGCCAAAGTGTTTGTGTTCCCGGAGTGCACCGGCAACATATCTCTATGAGTTCCTTGAGCGGAGGGGATGCTTTAATGACAGAAGACCCTGTTGGAGTGCTAAAGAGAGCTTGCGGAAGCAGTAGTAGCCGTAGCTCTTCACAGGATGGGGGTATCTTTAGCGATAAAGCCACTTTCAGGGATGAAAAGTTATGGTTCAGCATGCTACCAAATTGTTCTTTAGTAGTAGCCGTAGCTCTTCACAGGATGGGGGTATCTTTACCGATAGAGCCACTTTCAGGGATGAAAAGTTAA

Protein Analysis

179

Amino Acids

19.05

Weight (kDa)

6.98

Isoelectric Point (pI)

58.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAF_NLP PF22922 1 - 56 4.4e-17 NLP protein GAF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 303
AciI CCGC 3 cut(s) 183, 303, 357
AclWI GGATC 2 cut(s) 61, 95
AcoI YGGCCR 1 cut(s) 244
AfiI CCNNNNNNNGG 2 cut(s) 420, 521
AflIII ACRYGT 1 cut(s) 169
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 4 cut(s) 59, 353, 378, 479
AluI AGCT 4 cut(s) 59, 353, 378, 479
Alw21I GWGCWC 1 cut(s) 273
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 2 cut(s) 61, 95
AlwNI CAGNNNCTG 2 cut(s) 104, 124
AoxI GGCC 2 cut(s) 7, 244
ApaLI GTGCAC 1 cut(s) 269
Asp700I GAANNNNTTC 1 cut(s) 131
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 2 cut(s) 184, 207
BaeGI GKGCMC 1 cut(s) 273
BalI TGGCCA 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 273
BccI CCATC 3 cut(s) 226, 383, 484
BceAI ACGGC 2 cut(s) 357, 458
BcnI CCSGG 1 cut(s) 264
BfaI CTAG 1 cut(s) 71
BisI GCNGC 1 cut(s) 184
BlsI GCNGC 1 cut(s) 185
Bme1390I CCNGG 1 cut(s) 264
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 111, 301
BpiI GAAGAC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 319
BpuMI CCSGG 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 87, 196
Bsc4I CCNNNNNNNGG 2 cut(s) 420, 521
Bse118I RCCGGY 1 cut(s) 273
Bse1I ACTGG 2 cut(s) 55, 184
BseDI CCNNGG 2 cut(s) 87, 196
BseGI GGATG 5 cut(s) 316, 394, 430, 495, 531
BseLI CCNNNNNNNGG 2 cut(s) 420, 521
BseNI ACTGG 2 cut(s) 55, 184
BseSI GKGCMC 1 cut(s) 273
BshFI GGCC 2 cut(s) 9, 246
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 3 cut(s) 160, 264, 274
BslI CCNNNNNNNGG 2 cut(s) 420, 521
BsnI GGCC 2 cut(s) 9, 246
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 2 cut(s) 53, 100
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 3 cut(s) 183, 303, 357
BspANI GGCC 2 cut(s) 9, 246
BspLI GGNNCC 1 cut(s) 202
BspPI GGATC 2 cut(s) 61, 95
BspQI GCTCTTC 2 cut(s) 385, 486
BsrBI CCGCTC 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 273
BsrI ACTGG 2 cut(s) 55, 184
BssAI RCCGGY 1 cut(s) 273
BssECI CCNNGG 2 cut(s) 87, 196
BssMI GATC 2 cut(s) 53, 100
BssT1I CCWWGG 2 cut(s) 87, 196
Bst6I CTCTTC 2 cut(s) 385, 486
BstC8I GCNNGC 3 cut(s) 157, 355, 446
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 5 cut(s) 316, 394, 430, 495, 531
BstKTI GATC 2 cut(s) 56, 103
BstMBI GATC 2 cut(s) 53, 100
BstMWI GCNNNNNNNGC 2 cut(s) 23, 369
BstNSI RCATGY 2 cut(s) 173, 448
BstSCI CCNGG 1 cut(s) 262
BstSLI GKGCMC 1 cut(s) 273
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 2 cut(s) 9, 246
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 5 cut(s) 316, 394, 430, 495, 531
Cac8I GCNNGC 3 cut(s) 157, 355, 446
CaiI CAGNNNCTG 2 cut(s) 104, 124
Cfr10I RCCGGY 1 cut(s) 273
CviAII CATG 4 cut(s) 14, 170, 197, 445
DdeI CTNAG 1 cut(s) 38
DpnI GATC 2 cut(s) 55, 102
DpnII GATC 2 cut(s) 53, 100
EaeI YGGCCR 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 385, 486
EarI CTCTTC 2 cut(s) 385, 486
Eco130I CCWWGG 2 cut(s) 87, 196
EcoT14I CCWWGG 2 cut(s) 87, 196
ErhI CCWWGG 2 cut(s) 87, 196
FaeI CATG 4 cut(s) 17, 173, 200, 448
FaiI YATR 9 cut(s) 15, 48, 171, 188, 198, 282, 290, 436, 446
FatI CATG 4 cut(s) 13, 169, 196, 444
Fnu4HI GCNGC 1 cut(s) 184
FokI GGATG 4 cut(s) 323, 401, 437, 502
Fsp4HI GCNGC 1 cut(s) 184
FspBI CTAG 1 cut(s) 71
GluI GCNGC 1 cut(s) 184
HaeIII GGCC 2 cut(s) 9, 246
HapII CCGG 3 cut(s) 160, 264, 274
Hin1II CATG 4 cut(s) 17, 173, 200, 448
HpaII CCGG 3 cut(s) 160, 264, 274
HphI GGTGA 2 cut(s) 184, 207
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 1 cut(s) 237
Hpy188III TCNNGA 2 cut(s) 71, 104
Hpy8I GTNNAC 1 cut(s) 271
HpyAV CCTTC 1 cut(s) 20
HpyCH4V TGCA 3 cut(s) 138, 231, 271
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 369
HpyF3I CTNAG 1 cut(s) 38
Hsp92II CATG 4 cut(s) 17, 173, 200, 448
Kzo9I GATC 2 cut(s) 53, 100
LguI GCTCTTC 2 cut(s) 385, 486
LweI GCATC 2 cut(s) 111, 301
MaeI CTAG 1 cut(s) 71
MaeIII GTNAC 3 cut(s) 142, 165, 213
MalI GATC 2 cut(s) 55, 102
MbiI CCGCTC 1 cut(s) 303
MboI GATC 2 cut(s) 53, 100
MboII GAAGA 3 cut(s) 338, 372, 473
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 273
MlsI TGGCCA 1 cut(s) 246
MluCI AATT 2 cut(s) 27, 454
MluNI TGGCCA 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 3 cut(s) 156, 235, 299
Mox20I TGGCCA 1 cut(s) 246
MroXI GAANNNNTTC 1 cut(s) 131
MscI TGGCCA 1 cut(s) 246
MseI TTAA 2 cut(s) 317, 535
Msp20I TGGCCA 1 cut(s) 246
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 3 cut(s) 160, 264, 274
MspR9I CCNGG 1 cut(s) 264
MwoI GCNNNNNNNGC 2 cut(s) 23, 369
NciI CCSGG 1 cut(s) 264
NcoI CCATGG 1 cut(s) 196
NdeII GATC 2 cut(s) 53, 100
NlaIII CATG 4 cut(s) 17, 173, 200, 448
NlaIV GGNNCC 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 213
NspI RCATGY 2 cut(s) 173, 448
PaeI GCATGC 1 cut(s) 448
PciI ACATGT 1 cut(s) 169
PciSI GCTCTTC 2 cut(s) 385, 486
PdmI GAANNNNTTC 1 cut(s) 131
PfoI TCCNGGA 1 cut(s) 262
PkrI GCNGC 1 cut(s) 185
PscI ACATGT 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 202
PstNI CAGNNNCTG 2 cut(s) 104, 124
PsuI RGATCY 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 59
SapI GCTCTTC 2 cut(s) 385, 486
SaqAI TTAA 2 cut(s) 317, 535
SatI GCNGC 1 cut(s) 184
Sau3AI GATC 2 cut(s) 53, 100
ScrFI CCNGG 1 cut(s) 264
SduI GDGCHC 1 cut(s) 273
SetI ASST 5 cut(s) 61, 167, 355, 380, 481
SfaNI GCATC 2 cut(s) 111, 301
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
SphI GCATGC 1 cut(s) 448
Sse9I AATT 2 cut(s) 27, 454
SsiI CCGC 3 cut(s) 183, 303, 357
SspMI CTAG 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 262
StyI CCWWGG 2 cut(s) 87, 196
TasI AATT 2 cut(s) 27, 454
TauI GCSGC 1 cut(s) 186
Tru1I TTAA 2 cut(s) 317, 535
Tru9I TTAA 2 cut(s) 317, 535
TseFI GTSAC 1 cut(s) 213
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 1 cut(s) 441
VneI GTGCAC 1 cut(s) 269
XbaI TCTAGA 1 cut(s) 70
XceI RCATGY 2 cut(s) 173, 448
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.