MD06G1035100.v1.1

glycine-rich cell wall structural protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016405
Physical Location & Seq
Reverse (-)
4383191 .. 4383952
762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1035100.v1.1.491

Sequence Viewer

Length: 762 bp
ATGGAGATATGTTCTGCAGCTAGAACACTCCTAAATTATGAAGAGCCTGTCCACGTGCCTGCTGTCAGCTATGGGGCAGGCCATGGCATTGGGGGTGGTGCTGGATCAGGGTATGGCAGCGGTGCTGGAGGTTATGGTGGCGGAGGAGGTGCAGGAAGTGGTGGTGGCTATGGAGCTGTAGGAGAGCATGGCGCTGCTGGATATGGAGGTGGTAGTGGTGGTGGAGAAGGTGGTGGTGTTGCCTATGGAGGTGCTGGTGGACATGGTGGTGGCGGCGGTGGAGGCAGTGGTGGTGGTGGTGCATATGGTGCCGGGGGCGCAGAAGGGTATGGAAGTGGGGGTGGTAAAGGAGGTGGGGCTGGCTACGGTGCTGGTGGAGAACATGGGGCTGGTGGTGGTGGAGGAGGAGGTACTGGTGGTGGCGGAGGGTCCGGATATGCTGGAGAGCATGGCGGTGGTTATGGAGGAGGAGAAGGTGGTGGTGCTGGTGGGGGTTATGGCGCTGGTGGAGAACATGGTGGAGGATATGGTGGTGGCGGTGGGCATGGTAGTGGTGCTGGAGGAGGTTATGCTGCTGGAGGAGCAAGTGGAGGAGGATATGGGAGCGGTGGAGGAGCTGGAGGTGGAGTTGGTGGTGGTGGCGGCTATGCAGGTGGTGGTGGTGGTGGTGGAGGTTCTGGTGGCGGCAGCGGCTATGGTGGAGCGCATGGAGGAGGAGGTGGGGGTGGTGAAGGTGGTGGTCATGGTGGTTATGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

254

Amino Acids

19.95

Weight (kDa)

5.4

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016405)

Species Orthologous Gene IDs
malus_domestica MD06G1035100.v1.1 MD16G1273700.v1.1
prunus_persica Prupe.5G034800_v2.0.a1 Prupe.5G034900_v2.0.a1
pyrus_communis pycom06g02900
rosa_chinensis RchiOBHm_Chr7g0209821
rosa_laevigata RLG00000003087
rosa_multiflora Rmu_co8510463.1_g000001
rosa_roxburghii Rroxscaffold_3G00248920
rosa_rugosa Rorug07G0116400
rosa_samantha Rh7AG250400 Rh7BG244500 Rh7CG266400 Rh7DG256200
rosa_wichuraiana Rw7G021220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 641
Acc36I ACCTGC 1 cut(s) 641
AccB1I GGYRCC 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 606
AccIII TCCGGA 1 cut(s) 431
AclWI GGATC 1 cut(s) 112
AcvI CACGTG 1 cut(s) 55
AfaI GTAC 1 cut(s) 412
AluBI AGCT 4 cut(s) 20, 69, 176, 617
AluI AGCT 4 cut(s) 20, 69, 176, 617
AlwI GGATC 1 cut(s) 112
Aor13HI TCCGGA 1 cut(s) 431
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 5 cut(s) 17, 117, 194, 572, 687
AspLEI GCGC 4 cut(s) 194, 320, 503, 706
AspS9I GGNCC 1 cut(s) 429
AsuC2I CCSGG 1 cut(s) 313
AsuHPI GGTGA 1 cut(s) 740
AvaII GGWCC 1 cut(s) 429
BanI GGYRCC 1 cut(s) 308
BbrPI CACGTG 1 cut(s) 55
BbvI GCAGC 5 cut(s) 29, 129, 181, 559, 699
BcnI CCSGG 1 cut(s) 313
BfaI CTAG 1 cut(s) 21
BfmI CTRYAG 2 cut(s) 15, 177
BfoI RGCGCY 2 cut(s) 195, 504
BfuAI ACCTGC 1 cut(s) 641
BisI GCNGC 9 cut(s) 18, 118, 195, 274, 573, 643, 685, 688, 691
BlsI GCNGC 9 cut(s) 19, 119, 196, 275, 574, 644, 686, 689, 692
Bme1390I CCNGG 1 cut(s) 313
Bme18I GGWCC 1 cut(s) 429
BmgT120I GGNCC 1 cut(s) 429
BmiI GGNNCC 2 cut(s) 310, 430
BmrFI CCNGG 1 cut(s) 313
BpmI CTGGAG 5 cut(s) 147, 462, 579, 597, 639
BpuMI CCSGG 1 cut(s) 313
BsaAI YACGTR 1 cut(s) 55
BsaJI CCNNGG 2 cut(s) 82, 312
BsaWI WCCGGW 1 cut(s) 431
BsaXI ACNNNNNCTCC 2 cut(s) 612, 642
Bse1I ACTGG 1 cut(s) 418
BseAI TCCGGA 1 cut(s) 431
BseDI CCNNGG 2 cut(s) 82, 312
BseNI ACTGG 1 cut(s) 418
BseXI GCAGC 5 cut(s) 29, 129, 181, 559, 699
BsgI GTGCAG 1 cut(s) 171
BshFI GGCC 1 cut(s) 81
BshNI GGYRCC 1 cut(s) 308
BsiSI CCGG 2 cut(s) 312, 432
BsnI GGCC 1 cut(s) 81
Bsp13I TCCGGA 1 cut(s) 431
Bsp143I GATC 1 cut(s) 104
Bsp19I CCATGG 1 cut(s) 82
BspANI GGCC 1 cut(s) 81
BspEI TCCGGA 1 cut(s) 431
BspLI GGNNCC 2 cut(s) 310, 430
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 641
BspPI GGATC 1 cut(s) 112
BspQI GCTCTTC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 308
BsrBI CCGCTC 1 cut(s) 606
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 2 cut(s) 82, 312
BssMI GATC 1 cut(s) 104
BssT1I CCWWGG 1 cut(s) 82
Bst4CI ACNGT 1 cut(s) 368
Bst6I CTCTTC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 3 cut(s) 60, 79, 361
BstDSI CCRYGG 1 cut(s) 82
BstH2I RGCGCY 2 cut(s) 195, 504
BstHHI GCGC 4 cut(s) 194, 320, 503, 706
BstKTI GATC 1 cut(s) 107
BstMBI GATC 1 cut(s) 104
BstMWI GCNNNNNNNGC 5 cut(s) 282, 308, 317, 581, 690
BstSCI CCNGG 1 cut(s) 311
BstSFI CTRYAG 2 cut(s) 15, 177
BstV1I GCAGC 5 cut(s) 29, 129, 181, 559, 699
BstXI CCANNNNNNTGG 1 cut(s) 89
BsuRI GGCC 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 82
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 292
BveI ACCTGC 1 cut(s) 641
Cac8I GCNNGC 3 cut(s) 60, 79, 361
CfoI GCGC 4 cut(s) 194, 320, 503, 706
Cfr13I GGNCC 1 cut(s) 429
Csp6I GTAC 1 cut(s) 411
CviAII CATG 9 cut(s) 83, 188, 263, 383, 449, 515, 545, 707, 743
CviQI GTAC 1 cut(s) 411
DpnI GATC 1 cut(s) 106
DpnII GATC 1 cut(s) 104
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EciI GGCGGA 2 cut(s) 156, 438
Eco130I CCWWGG 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 429
Eco72I CACGTG 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 9 cut(s) 86, 191, 266, 386, 452, 518, 548, 710, 746
FatI CATG 9 cut(s) 82, 187, 262, 382, 448, 514, 544, 706, 742
FauNDI CATATG 1 cut(s) 304
Fnu4HI GCNGC 9 cut(s) 18, 118, 195, 274, 573, 643, 685, 688, 691
Fsp4HI GCNGC 9 cut(s) 18, 118, 195, 274, 573, 643, 685, 688, 691
FspBI CTAG 1 cut(s) 21
GlaI GCGC 4 cut(s) 193, 319, 502, 705
GluI GCNGC 9 cut(s) 18, 118, 195, 274, 573, 643, 685, 688, 691
GsuI CTGGAG 5 cut(s) 147, 462, 579, 597, 639
HaeII RGCGCY 2 cut(s) 195, 504
HaeIII GGCC 1 cut(s) 81
HapII CCGG 2 cut(s) 312, 432
HhaI GCGC 4 cut(s) 194, 320, 503, 706
Hin1II CATG 9 cut(s) 86, 191, 266, 386, 452, 518, 548, 710, 746
Hin6I GCGC 4 cut(s) 192, 318, 501, 704
HinP1I GCGC 4 cut(s) 192, 318, 501, 704
HpaII CCGG 2 cut(s) 312, 432
HphI GGTGA 1 cut(s) 740
Hpy166II GTNNAC 2 cut(s) 52, 260
Hpy188III TCNNGA 1 cut(s) 432
Hpy8I GTNNAC 2 cut(s) 52, 260
HpyAV CCTTC 4 cut(s) 221, 317, 467, 725
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 4 cut(s) 17, 152, 302, 650
HpyF10VI GCNNNNNNNGC 5 cut(s) 282, 308, 317, 581, 690
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 9 cut(s) 86, 191, 266, 386, 452, 518, 548, 710, 746
HspAI GCGC 4 cut(s) 192, 318, 501, 704
Kpn2I TCCGGA 1 cut(s) 431
Kzo9I GATC 1 cut(s) 104
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 5 cut(s) 173, 581, 603, 614, 701
Lsp1109I GCAGC 5 cut(s) 29, 129, 181, 559, 699
MaeI CTAG 1 cut(s) 21
MaeII ACGT 1 cut(s) 54
MalI GATC 1 cut(s) 106
MbiI CCGCTC 1 cut(s) 606
MboI GATC 1 cut(s) 104
MboII GAAGA 1 cut(s) 53
MluCI AATT 1 cut(s) 34
MroI TCCGGA 1 cut(s) 431
MslI CAYNNNNRTG 3 cut(s) 267, 453, 549
MspA1I CMGCKG 2 cut(s) 120, 690
MspI CCGG 2 cut(s) 312, 432
MspR9I CCNGG 1 cut(s) 313
MwoI GCNNNNNNNGC 5 cut(s) 282, 308, 317, 581, 690
NciI CCSGG 1 cut(s) 313
NcoI CCATGG 1 cut(s) 82
NdeI CATATG 1 cut(s) 304
NdeII GATC 1 cut(s) 104
NlaIII CATG 9 cut(s) 86, 191, 266, 386, 452, 518, 548, 710, 746
NlaIV GGNNCC 2 cut(s) 310, 430
PaqCI CACCTGC 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 36
PkrI GCNGC 9 cut(s) 19, 119, 196, 275, 574, 644, 686, 689, 692
PmaCI CACGTG 1 cut(s) 55
PmlI CACGTG 1 cut(s) 55
Ppu21I YACGTR 1 cut(s) 55
PspCI CACGTG 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 310, 430
PspPI GGNCC 1 cut(s) 429
PstI CTGCAG 1 cut(s) 19
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 3 cut(s) 267, 453, 549
SapI GCTCTTC 1 cut(s) 36
SatI GCNGC 9 cut(s) 18, 118, 195, 274, 573, 643, 685, 688, 691
Sau3AI GATC 1 cut(s) 104
Sau96I GGNCC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 313
SfcI CTRYAG 2 cut(s) 15, 177
SinI GGWCC 1 cut(s) 429
SmiMI CAYNNNNRTG 3 cut(s) 267, 453, 549
Sse9I AATT 1 cut(s) 34
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 311
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 368
TaiI ACGT 1 cut(s) 57
TasI AATT 1 cut(s) 34
TauI GCSGC 4 cut(s) 276, 645, 687, 693
TscAI CASTG 1 cut(s) 292
TseI GCWGC 5 cut(s) 17, 117, 194, 572, 687
TspDTI ATGAA 1 cut(s) 54
TspRI CASTG 1 cut(s) 292
VpaK11BI GGWCC 1 cut(s) 429
XspI CTAG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.