Rh7DG256200

glycine-rich cell wall structural protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0016405
Physical Location & Seq
Reverse (-)
26775638 .. 26776849
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG256200.1

Sequence Viewer

Length: 921 bp
ATGCGTTACAGGACCCCCTTCCCATCATTCCTATATATACCTAGCTACCTTCTCTTGCATTCTACACCAGGCACCACCATTGTTGTGGGTTTCATCTGCATTGCTTCGATGGCTTCTCAAAGAGTTCAGAGTCTTGTTTTCTTCGTGTTATTAGGTCTAGGCATATGTTCTGCAGCAAGAACACTCCTGACTTATGAAACGTATCCTGCTATCGGCCATGGTGGCGGGGGTGGATCCGGGTATGGAGGTGGTGGTGCTGGAGGTGCAGGTGGTGGCGGAGGAGGTGGAGGCAGTGGAGGTGGTTATGGCGCTGTAGGTGAACATGGTGCTGCTGGCTATGGAGGCGGTAGTGGTGGTGGAGAAGGCGGTGGTGTTGCGTATGGAGGCGCTGGTGGACATGCCGGTGGTGGAGGAGGTGGCGGTGGAAGTGGTGGTGGCGGTGCATATGGCACTGGAGGGGCTTATGGTGCTGGCGGTGCTAATGGTTATGGAAGCGGCGGTGGTGAAGGAGGTGGTGCCGGGTATGGTGGAGCTGGTGGTGCTGGTGGAGGCGGTGCTGGTGGTGGTTCTGGTGGTGGTGGGGGAGCTGCAGATGGAGGAGAGCACGGCACTGGTTATGGAGGTGGAGAAGGTGGTGGTGCCGGTGGAGGCTATGGTGCTGGCGGCGAGCATGGTGCCGGATATGGAGGTGGTGGGGGTCACGGTGGTGGCGGAGGTTACGGTGCTGGAGGAGCAAGTGGAGGTGGATATGGAGGTGGCGGAGGAGCTGGAGGTGGAGCCGGAGGAGCTGCCGGTGGGTATGCTGGTGGTGGGGGCGGAGGCGCTGGAAGCGGTGGTGGTGGTGGTGGTGCACATGGCGGTGCATATGGAGGAGGTGGCGGGAGCGGTGAGGGTGGTGGTCATGGTGGTTATATTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

306

Amino Acids

25.22

Weight (kDa)

5.92

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016405)

Species Orthologous Gene IDs
malus_domestica MD06G1035100.v1.1 MD16G1273700.v1.1
prunus_persica Prupe.5G034800_v2.0.a1 Prupe.5G034900_v2.0.a1
pyrus_communis pycom06g02900
rosa_chinensis RchiOBHm_Chr7g0209821
rosa_laevigata RLG00000003087
rosa_multiflora Rmu_co8510463.1_g000001
rosa_roxburghii Rroxscaffold_3G00248920
rosa_rugosa Rorug07G0116400
rosa_samantha Rh7AG250400 Rh7BG244500 Rh7CG266400 Rh7DG256200
rosa_wichuraiana Rw7G021220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 257
AccB1I GGYRCC 4 cut(s) 71, 515, 638, 674
AccBSI CCGCTC 1 cut(s) 885
AclWI GGATC 2 cut(s) 228, 241
AcoI YGGCCR 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 212
AjnI CCWGG 1 cut(s) 67
AleI CACNNNNGTG 1 cut(s) 705
AluBI AGCT 5 cut(s) 45, 533, 587, 767, 788
AluI AGCT 5 cut(s) 45, 533, 587, 767, 788
Alw21I GWGCWC 2 cut(s) 606, 853
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 2 cut(s) 228, 241
AoxI GGCC 1 cut(s) 214
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 4 cut(s) 173, 329, 587, 788
AspLEI GCGC 3 cut(s) 311, 389, 824
AspS9I GGNCC 1 cut(s) 12
AsuC2I CCSGG 2 cut(s) 238, 520
AsuHPI GGTGA 3 cut(s) 329, 515, 899
AvaII GGWCC 1 cut(s) 12
BaeGI GKGCMC 1 cut(s) 853
BamHI GGATCC 1 cut(s) 233
BanI GGYRCC 4 cut(s) 71, 515, 638, 674
Bbv12I GWGCWC 2 cut(s) 606, 853
BbvI GCAGC 4 cut(s) 185, 316, 574, 775
BccI CCATC 3 cut(s) 31, 103, 587
BceAI ACGGC 1 cut(s) 622
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 1 cut(s) 69
BciVI GTATCC 1 cut(s) 213
BcnI CCSGG 2 cut(s) 238, 520
BfaI CTAG 2 cut(s) 42, 158
BfmI CTRYAG 3 cut(s) 171, 312, 588
BfoI RGCGCY 3 cut(s) 312, 390, 825
BfuAI ACCTGC 1 cut(s) 257
BfuI GTATCC 1 cut(s) 213
BglI GCCNNNNNGGC 1 cut(s) 222
BisI GCNGC 6 cut(s) 174, 330, 496, 588, 664, 789
BlsI GCNGC 6 cut(s) 175, 331, 497, 589, 665, 790
Bme1390I CCNGG 3 cut(s) 69, 238, 520
Bme18I GGWCC 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 7 cut(s) 14, 73, 235, 517, 640, 676, 778
BmrFI CCNGG 3 cut(s) 69, 238, 520
BpmI CTGGAG 4 cut(s) 279, 474, 747, 789
BpuMI CCSGG 2 cut(s) 238, 520
BsaJI CCNNGG 1 cut(s) 217
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse118I RCCGGY 3 cut(s) 401, 641, 791
Bse1I ACTGG 2 cut(s) 457, 616
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 69
BseDI CCNNGG 1 cut(s) 217
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMI GCAATG 1 cut(s) 99
BseNI ACTGG 2 cut(s) 457, 616
BseRI GAGGAG 7 cut(s) 294, 426, 612, 744, 777, 798, 885
BseSI GKGCMC 1 cut(s) 853
BseXI GCAGC 4 cut(s) 185, 316, 574, 775
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 1 cut(s) 216
BshNI GGYRCC 4 cut(s) 71, 515, 638, 674
BsiHKAI GWGCWC 2 cut(s) 606, 853
BsiSI CCGG 7 cut(s) 237, 402, 519, 642, 678, 780, 792
BslI CCNNNNNNNGG 1 cut(s) 212
BsmI GAATGC 1 cut(s) 58
BsnI GGCC 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 606, 853
Bsp143I GATC 1 cut(s) 233
Bsp19I CCATGG 1 cut(s) 217
BspANI GGCC 1 cut(s) 216
BspLI GGNNCC 7 cut(s) 14, 73, 235, 517, 640, 676, 778
BspMAI CTGCAG 2 cut(s) 175, 592
BspMI ACCTGC 1 cut(s) 257
BspPI GGATC 2 cut(s) 228, 241
BspT107I GGYRCC 4 cut(s) 71, 515, 638, 674
BsrBI CCGCTC 1 cut(s) 885
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 3 cut(s) 401, 641, 791
BsrI ACTGG 2 cut(s) 457, 616
BssAI RCCGGY 3 cut(s) 401, 641, 791
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 69
Bst4CI ACNGT 2 cut(s) 704, 722
BstC8I GCNNGC 4 cut(s) 334, 472, 661, 668
BstDSI CCRYGG 1 cut(s) 217
BstH2I RGCGCY 3 cut(s) 312, 390, 825
BstHHI GCGC 3 cut(s) 311, 389, 824
BstKTI GATC 1 cut(s) 236
BstMBI GATC 1 cut(s) 233
BstNI CCWGG 1 cut(s) 69
BstNSI RCATGY 1 cut(s) 401
BstSCI CCNGG 3 cut(s) 67, 236, 518
BstSFI CTRYAG 3 cut(s) 171, 312, 588
BstSLI GKGCMC 1 cut(s) 853
BstV1I GCAGC 4 cut(s) 185, 316, 574, 775
BstX2I RGATCY 1 cut(s) 233
BstXI CCANNNNNNTGG 1 cut(s) 85
BstYI RGATCY 1 cut(s) 233
BsuI GTATCC 1 cut(s) 213
BsuRI GGCC 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 217
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 3 cut(s) 298, 450, 609
BveI ACCTGC 1 cut(s) 257
Cac8I GCNNGC 4 cut(s) 334, 472, 661, 668
CfoI GCGC 3 cut(s) 311, 389, 824
Cfr10I RCCGGY 3 cut(s) 401, 641, 791
Cfr13I GGNCC 1 cut(s) 12
CspCI CAANNNNNGTGG 2 cut(s) 64, 99
CviAII CATG 6 cut(s) 218, 323, 398, 671, 854, 902
DpnI GATC 1 cut(s) 235
DpnII GATC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 214
EciI GGCGGA 4 cut(s) 291, 726, 774, 831
Eco130I CCWWGG 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 6 cut(s) 221, 326, 401, 674, 857, 905
FatI CATG 6 cut(s) 217, 322, 397, 670, 853, 901
FauI CCCGC 2 cut(s) 218, 872
FauNDI CATATG 3 cut(s) 164, 445, 865
Fnu4HI GCNGC 6 cut(s) 174, 330, 496, 588, 664, 789
Fsp4HI GCNGC 6 cut(s) 174, 330, 496, 588, 664, 789
FspBI CTAG 2 cut(s) 42, 158
GlaI GCGC 3 cut(s) 310, 388, 823
GluI GCNGC 6 cut(s) 174, 330, 496, 588, 664, 789
GsuI CTGGAG 4 cut(s) 279, 474, 747, 789
HaeII RGCGCY 3 cut(s) 312, 390, 825
HaeIII GGCC 1 cut(s) 216
HapII CCGG 7 cut(s) 237, 402, 519, 642, 678, 780, 792
HhaI GCGC 3 cut(s) 311, 389, 824
Hin1II CATG 6 cut(s) 221, 326, 401, 674, 857, 905
Hin6I GCGC 3 cut(s) 309, 387, 822
HinP1I GCGC 3 cut(s) 309, 387, 822
HinfI GANTC 1 cut(s) 130
HpaII CCGG 7 cut(s) 237, 402, 519, 642, 678, 780, 792
HphI GGTGA 3 cut(s) 329, 515, 899
Hpy166II GTNNAC 3 cut(s) 320, 395, 851
Hpy188I TCNGA 1 cut(s) 129
Hpy188III TCNNGA 1 cut(s) 187
Hpy8I GTNNAC 3 cut(s) 320, 395, 851
HpyAV CCTTC 5 cut(s) 28, 59, 356, 500, 623
HpyCH4III ACNGT 2 cut(s) 704, 722
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 8 cut(s) 58, 99, 173, 266, 443, 590, 851, 863
HpySE526I ACGT 1 cut(s) 200
Hsp92II CATG 6 cut(s) 221, 326, 401, 674, 857, 905
HspAI GCGC 3 cut(s) 309, 387, 822
Kzo9I GATC 1 cut(s) 233
LmnI GCTCC 7 cut(s) 530, 584, 731, 764, 776, 785, 882
Lsp1109I GCAGC 4 cut(s) 185, 316, 574, 775
MaeI CTAG 2 cut(s) 42, 158
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 3 cut(s) 5, 698, 716
MalI GATC 1 cut(s) 235
MbiI CCGCTC 1 cut(s) 885
MboI GATC 1 cut(s) 233
MboII GAAGA 1 cut(s) 133
MflI RGATCY 1 cut(s) 233
MhlI GDGCHC 2 cut(s) 606, 853
MlyI GAGTC 1 cut(s) 139
MslI CAYNNNNRTG 4 cut(s) 83, 402, 705, 858
MspI CCGG 7 cut(s) 237, 402, 519, 642, 678, 780, 792
MspR9I CCNGG 3 cut(s) 69, 238, 520
Mva1269I GAATGC 1 cut(s) 58
MvaI CCWGG 1 cut(s) 69
NciI CCSGG 2 cut(s) 238, 520
NcoI CCATGG 1 cut(s) 217
NdeI CATATG 3 cut(s) 164, 445, 865
NdeII GATC 1 cut(s) 233
NlaIII CATG 6 cut(s) 221, 326, 401, 674, 857, 905
NlaIV GGNNCC 7 cut(s) 14, 73, 235, 517, 640, 676, 778
NmuCI GTSAC 1 cut(s) 698
NspI RCATGY 1 cut(s) 401
OliI CACNNNNGTG 1 cut(s) 705
PaqCI CACCTGC 1 cut(s) 257
PctI GAATGC 1 cut(s) 58
PkrI GCNGC 6 cut(s) 175, 331, 497, 589, 665, 790
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
PpuMI RGGWCCY 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 67
PspGI CCWGG 1 cut(s) 67
PspN4I GGNNCC 7 cut(s) 14, 73, 235, 517, 640, 676, 778
PspPI GGNCC 1 cut(s) 12
PspPPI RGGWCCY 1 cut(s) 12
PstI CTGCAG 2 cut(s) 175, 592
PsuI RGATCY 1 cut(s) 233
RseI CAYNNNNRTG 4 cut(s) 83, 402, 705, 858
SatI GCNGC 6 cut(s) 174, 330, 496, 588, 664, 789
Sau3AI GATC 1 cut(s) 233
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 3 cut(s) 69, 238, 520
SduI GDGCHC 2 cut(s) 606, 853
SfcI CTRYAG 3 cut(s) 171, 312, 588
SinI GGWCC 1 cut(s) 12
SmiMI CAYNNNNRTG 4 cut(s) 83, 402, 705, 858
SspMI CTAG 2 cut(s) 42, 158
StyD4I CCNGG 3 cut(s) 67, 236, 518
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 2 cut(s) 704, 722
TaiI ACGT 1 cut(s) 203
TaqI TCGA 1 cut(s) 107
TauI GCSGC 2 cut(s) 498, 666
TscAI CASTG 3 cut(s) 298, 457, 616
TseFI GTSAC 1 cut(s) 698
TseI GCWGC 4 cut(s) 173, 329, 587, 788
Tsp45I GTSAC 1 cut(s) 698
TspDTI ATGAA 2 cut(s) 82, 210
TspRI CASTG 3 cut(s) 298, 457, 616
VneI GTGCAC 1 cut(s) 849
VpaK11BI GGWCC 1 cut(s) 12
XceI RCATGY 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 82
XspI CTAG 2 cut(s) 42, 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.