MD06G1073200.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013870
Physical Location & Seq
Reverse (-)
18192935 .. 18195751
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1073200.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGTCTCTCCATCTTATTCTCACACATCAGGCCGCTGTCCTCCCTCCTTTTCTAAGGAACCAAACAAACAGCCAAACACAATCCTCCCACAGCTTGTATTCAACGCGTCCCTCCCCTCTACACATCATCAACAAGTTTCATACTTTTTGCCACCGTTAAATCCTCCATGTCCACTCCCACCATACCCACCTCTCTCTCTCCTCTATTCTCCTAGCTTTCTCTCTCTTTCTTTCTCTAGGATTGCTGTCTACTGTTTGACTATGGGTGCGGGTTTCTCTTCTGTCTTCAAATCGGAAAACCCAGTTACAGTCGGATCATTGCCATCGAAACCCGGTCTCGGCGATTTGCCGGAGAGCTGCGCGGCGATGATTCTGGGGTATTTGGACCCGCCGGAGATTTGCAAATTGGCGAAGCTGAATCGGGCTTTTCGCGGCGCTTCTTGGGCTGATTTCATTTGGGAATCGAAATTGCCGTCGAATTATCAAACTATTGTGGGCAAAGTGTTCGGCGATGGATTGGAAAATCTGGGTAAGAGAGATGTTTATACGAGGCTTTGTCAGCCCAACTCTTTCGATGATGGAACCAAGCGTGGGAAAATTGGGAAAACGGTTTGGCTGGATAAAAGTACAGGATCAGTTTGCTTGTCGATTTCTTCTAAGGGATTAGCAATTACCGGGATTGATGATCGAAGATATTGGAATCATATTCCAACTGAAGAATCTAGATTTTGCTCAGTTGCATATCTTCAGCAAATATGGTGGTTTGAAGTTGACGGGGAGGTTGAGTTTCCATTTCCTGTTGGGACATACAGCCTATTCTTCAGGTTGCAGCTGGGACGGTCTTCTCAGAAAAGGTTTGGTCGCCGAGTTTGCAATATTGAGCATGTCCACGGTTGGGACATAAAACCCGTGAGGTTCCAACTATGGACTTCAGAAGGTCATTATGCCTCGTCTCAGTGCTTTTTAACGGAACCTGGAAAATGGAATTACTACCATGTTGGGGACTTTGTTGTGGAGAACCCGAATGCATCAACGAAGATTAAACTCTCGATGACCCAGATCGATTGCACCCACACCAAAGGCGGTCTCTGTTTAGACTCTGTACTTATATACCCTAGTGAATTTAGAGAGAGGCTAAAGCATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

383

Amino Acids

42.95

Weight (kDa)

8.73

Isoelectric Point (pI)

49.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 213 - 372 2.7e-37 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12710 AT1G12710 AT1G63090
fragaria_vesca FvH4_5g01540
malus_domestica MD04G1067200.v1.1 MD06G1073200.v1.1
prunus_persica Prupe.5G091600_v2.0.a1
pyrus_communis pycom04g06030
rosa_chinensis RchiOBHm_Chr7g0199151
rosa_laevigata RLG00000003810
rosa_multiflora Rmu_sc0006266.1_g000060
rosa_roxburghii Rroxscaffold_3G00257010
rosa_rugosa Rorug07G0053000
rosa_samantha Rh7BG180200 Rh7CG185800 Rh7DG179600
rosa_wichuraiana Rw7G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 249
AccII CGCG 3 cut(s) 107, 362, 432
AciI CCGC 6 cut(s) 34, 269, 362, 389, 432, 1083
AclWI GGATC 2 cut(s) 322, 640
AcsI RAATTY 1 cut(s) 1121
AcuI CTGAAG 4 cut(s) 731, 735, 805, 915
AfaI GTAC 2 cut(s) 628, 1104
AfiI CCNNNNNNNGG 4 cut(s) 338, 591, 895, 1000
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 3 cut(s) 103, 289, 767
AjnI CCWGG 1 cut(s) 973
AluBI AGCT 5 cut(s) 94, 216, 357, 415, 832
AluI AGCT 5 cut(s) 94, 216, 357, 415, 832
Alw26I GTCTC 4 cut(s) 10, 341, 957, 1091
AlwI GGATC 2 cut(s) 322, 640
AoxI GGCC 1 cut(s) 31
ApeKI GCWGC 2 cut(s) 357, 829
ApoI RAATTY 1 cut(s) 1121
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
AspLEI GCGC 2 cut(s) 362, 437
AspS9I GGNCC 1 cut(s) 385
AsuC2I CCSGG 2 cut(s) 333, 676
AvaII GGWCC 1 cut(s) 385
BbsI GAAGAC 2 cut(s) 277, 834
BbvI GCAGC 2 cut(s) 344, 841
BccI CCATC 4 cut(s) 19, 331, 506, 572
BceAI ACGGC 1 cut(s) 457
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 1 cut(s) 975
BcnI CCSGG 2 cut(s) 333, 676
BcoDI GTCTC 4 cut(s) 10, 341, 957, 1091
BfaI CTAG 4 cut(s) 213, 237, 723, 1116
BfoI RGCGCY 1 cut(s) 438
BisI GCNGC 5 cut(s) 34, 358, 363, 433, 830
BlsI GCNGC 5 cut(s) 35, 359, 364, 434, 831
Bme1390I CCNGG 3 cut(s) 333, 676, 975
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 5 cut(s) 60, 387, 583, 917, 972
BmrFI CCNGG 3 cut(s) 333, 676, 975
BmrI ACTGGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 1037
BmuI ACTGGG 1 cut(s) 296
BpiI GAAGAC 2 cut(s) 277, 834
BpuMI CCSGG 2 cut(s) 333, 676
Bsa29I ATCGAT 1 cut(s) 1062
BsaI GGTCTC 3 cut(s) 10, 341, 1091
BsaJI CCNNGG 1 cut(s) 889
BsaXI ACNNNNNCTCC 2 cut(s) 770, 800
Bsc4I CCNNNNNNNGG 4 cut(s) 338, 591, 895, 1000
Bse1I ACTGG 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 317
BseBI CCWGG 1 cut(s) 975
BseCI ATCGAT 1 cut(s) 1062
BseDI CCNNGG 1 cut(s) 889
BseLI CCNNNNNNNGG 4 cut(s) 338, 591, 895, 1000
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 3 cut(s) 747, 860, 968
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 2 cut(s) 344, 841
BseYI CCCAGC 1 cut(s) 832
Bsh1236I CGCG 3 cut(s) 107, 362, 432
BshFI GGCC 1 cut(s) 33
BshVI ATCGAT 1 cut(s) 1062
BsiSI CCGG 4 cut(s) 333, 350, 392, 675
BslFI GGGAC 5 cut(s) 94, 817, 849, 911, 1016
BslI CCNNNNNNNGG 4 cut(s) 338, 591, 895, 1000
BsmAI GTCTC 4 cut(s) 10, 341, 957, 1091
BsmBI CGTCTC 1 cut(s) 957
BsmFI GGGAC 5 cut(s) 94, 817, 849, 911, 1016
BsmI GAATGC 1 cut(s) 1030
BsnI GGCC 1 cut(s) 33
Bso31I GGTCTC 3 cut(s) 10, 341, 1091
Bsp143I GATC 4 cut(s) 314, 632, 685, 1059
BspACI CCGC 6 cut(s) 34, 269, 362, 389, 432, 1083
BspANI GGCC 1 cut(s) 33
BspCNI CTCAG 3 cut(s) 746, 859, 967
BspDI ATCGAT 1 cut(s) 1062
BspFNI CGCG 3 cut(s) 107, 362, 432
BspLI GGNNCC 5 cut(s) 60, 387, 583, 917, 972
BspPI GGATC 2 cut(s) 322, 640
BspTNI GGTCTC 3 cut(s) 10, 341, 1091
BsrDI GCAATG 1 cut(s) 317
BsrI ACTGG 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 889
BssMI GATC 4 cut(s) 314, 632, 685, 1059
Bst2UI CCWGG 1 cut(s) 975
Bst4CI ACNGT 6 cut(s) 156, 254, 310, 610, 840, 893
Bst6I CTCTTC 1 cut(s) 283
BstDEI CTNAG 5 cut(s) 54, 657, 733, 846, 954
BstDSI CCRYGG 1 cut(s) 889
BstFNI CGCG 3 cut(s) 107, 362, 432
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 2 cut(s) 362, 437
BstKTI GATC 4 cut(s) 317, 635, 688, 1062
BstMAI GTCTC 4 cut(s) 10, 341, 957, 1091
BstMBI GATC 4 cut(s) 314, 632, 685, 1059
BstMWI GCNNNNNNNGC 3 cut(s) 443, 559, 870
BstNI CCWGG 1 cut(s) 975
BstNSI RCATGY 1 cut(s) 887
BstSCI CCNGG 3 cut(s) 331, 674, 973
BstUI CGCG 3 cut(s) 107, 362, 432
BstV1I GCAGC 2 cut(s) 344, 841
BstV2I GAAGAC 2 cut(s) 277, 834
Bsu15I ATCGAT 1 cut(s) 1062
BsuRI GGCC 1 cut(s) 33
BsuTUI ATCGAT 1 cut(s) 1062
BtgI CCRYGG 1 cut(s) 889
BtgZI GCGATG 2 cut(s) 380, 525
BtsIMutI CAGTG 1 cut(s) 962
CfoI GCGC 2 cut(s) 362, 437
Cfr13I GGNCC 1 cut(s) 385
ClaI ATCGAT 1 cut(s) 1062
CseI GACGC 1 cut(s) 96
Csp6I GTAC 2 cut(s) 627, 1103
CspCI CAANNNNNGTGG 2 cut(s) 740, 775
CviAII CATG 3 cut(s) 168, 884, 995
CviQI GTAC 2 cut(s) 627, 1103
DdeI CTNAG 5 cut(s) 54, 657, 733, 846, 954
DpnI GATC 4 cut(s) 316, 634, 687, 1061
DpnII GATC 4 cut(s) 314, 632, 685, 1059
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
Eco31I GGTCTC 3 cut(s) 10, 341, 1091
Eco47I GGWCC 1 cut(s) 385
Eco57I CTGAAG 4 cut(s) 731, 735, 805, 915
EcoRII CCWGG 1 cut(s) 973
EcoT22I ATGCAT 1 cut(s) 1030
Esp3I CGTCTC 1 cut(s) 957
FaeI CATG 3 cut(s) 171, 887, 998
FaqI GGGAC 5 cut(s) 94, 817, 849, 911, 1016
FatI CATG 3 cut(s) 167, 883, 994
FauI CCCGC 2 cut(s) 262, 396
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 5 cut(s) 34, 358, 363, 433, 830
Fsp4HI GCNGC 5 cut(s) 34, 358, 363, 433, 830
FspBI CTAG 4 cut(s) 213, 237, 723, 1116
GlaI GCGC 2 cut(s) 361, 436
GluI GCNGC 5 cut(s) 34, 358, 363, 433, 830
GsaI CCCAGC 1 cut(s) 836
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 1 cut(s) 33
HapII CCGG 4 cut(s) 333, 350, 392, 675
HgaI GACGC 1 cut(s) 96
HhaI GCGC 2 cut(s) 362, 437
Hin1II CATG 3 cut(s) 171, 887, 998
Hin6I GCGC 2 cut(s) 360, 435
HinP1I GCGC 2 cut(s) 360, 435
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 6 cut(s) 370, 418, 461, 700, 719, 1097
HpaII CCGG 4 cut(s) 333, 350, 392, 675
Hpy166II GTNNAC 4 cut(s) 173, 250, 772, 889
Hpy188I TCNGA 4 cut(s) 295, 314, 849, 934
Hpy188III TCNNGA 2 cut(s) 723, 1048
Hpy8I GTNNAC 4 cut(s) 173, 250, 772, 889
Hpy99I CGWCG 1 cut(s) 478
HpyAV CCTTC 1 cut(s) 929
HpyCH4III ACNGT 6 cut(s) 156, 254, 310, 610, 840, 893
HpyCH4V TGCA 6 cut(s) 402, 740, 829, 873, 1028, 1068
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 559, 870
HpyF3I CTNAG 5 cut(s) 54, 657, 733, 846, 954
Hsp92II CATG 3 cut(s) 171, 887, 998
HspAI GCGC 2 cut(s) 360, 435
Kzo9I GATC 4 cut(s) 314, 632, 685, 1059
Lsp1109I GCAGC 2 cut(s) 344, 841
LweI GCATC 1 cut(s) 1037
MaeI CTAG 4 cut(s) 213, 237, 723, 1116
MaeIII GTNAC 1 cut(s) 304
MalI GATC 4 cut(s) 316, 634, 687, 1061
MboI GATC 4 cut(s) 314, 632, 685, 1059
MboII GAAGA 9 cut(s) 270, 277, 645, 702, 728, 737, 811, 834, 1048
MluCI AATT 7 cut(s) 404, 467, 478, 597, 669, 985, 1121
MluI ACGCGT 1 cut(s) 105
MlyI GAGTC 1 cut(s) 1091
MmeI TCCRAC 3 cut(s) 292, 734, 943
Mph1103I ATGCAT 1 cut(s) 1030
MseI TTAA 3 cut(s) 158, 965, 1041
MspA1I CMGCKG 2 cut(s) 36, 832
MspI CCGG 4 cut(s) 333, 350, 392, 675
MspR9I CCNGG 3 cut(s) 333, 676, 975
Mva1269I GAATGC 1 cut(s) 1030
MvaI CCWGG 1 cut(s) 975
MvnI CGCG 3 cut(s) 107, 362, 432
MwoI GCNNNNNNNGC 3 cut(s) 443, 559, 870
NciI CCSGG 2 cut(s) 333, 676
NdeII GATC 4 cut(s) 314, 632, 685, 1059
NlaIII CATG 3 cut(s) 171, 887, 998
NlaIV GGNNCC 5 cut(s) 60, 387, 583, 917, 972
NmeAIII GCCGAG 2 cut(s) 318, 890
NsiI ATGCAT 1 cut(s) 1030
NspI RCATGY 1 cut(s) 887
PcsI WCGNNNNNNNCGW 1 cut(s) 470
PctI GAATGC 1 cut(s) 1030
PfeI GAWTC 5 cut(s) 370, 418, 461, 700, 719
PkrI GCNGC 5 cut(s) 35, 359, 364, 434, 831
PleI GAGTC 1 cut(s) 1091
PpsI GAGTC 1 cut(s) 1091
Psp6I CCWGG 1 cut(s) 973
PspFI CCCAGC 1 cut(s) 832
PspGI CCWGG 1 cut(s) 973
PspN4I GGNNCC 5 cut(s) 60, 387, 583, 917, 972
PspPI GGNCC 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 832
RsaI GTAC 2 cut(s) 628, 1104
RsaNI GTAC 2 cut(s) 627, 1103
SaqAI TTAA 3 cut(s) 158, 965, 1041
SatI GCNGC 5 cut(s) 34, 358, 363, 433, 830
Sau3AI GATC 4 cut(s) 314, 632, 685, 1059
Sau96I GGNCC 1 cut(s) 385
SchI GAGTC 1 cut(s) 1091
ScrFI CCNGG 3 cut(s) 333, 676, 975
SfaNI GCATC 1 cut(s) 1037
SinI GGWCC 1 cut(s) 385
Sse9I AATT 7 cut(s) 404, 467, 478, 597, 669, 985, 1121
SsiI CCGC 6 cut(s) 34, 269, 362, 389, 432, 1083
SspI AATATT 1 cut(s) 877
SspMI CTAG 4 cut(s) 213, 237, 723, 1116
StyD4I CCNGG 3 cut(s) 331, 674, 973
TaaI ACNGT 6 cut(s) 156, 254, 310, 610, 840, 893
TaqI TCGA 8 cut(s) 326, 464, 476, 573, 647, 688, 1049, 1062
TasI AATT 7 cut(s) 404, 467, 478, 597, 669, 985, 1121
TatI WGTACW 2 cut(s) 626, 1102
TauI GCSGC 3 cut(s) 36, 365, 435
TfiI GAWTC 5 cut(s) 370, 418, 461, 700, 719
Tru1I TTAA 3 cut(s) 158, 965, 1041
Tru9I TTAA 3 cut(s) 158, 965, 1041
TscAI CASTG 1 cut(s) 962
TseI GCWGC 2 cut(s) 357, 829
TspDTI ATGAA 2 cut(s) 129, 442
TspGWI ACGGA 1 cut(s) 983
TspRI CASTG 1 cut(s) 962
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 1 cut(s) 1121
XbaI TCTAGA 1 cut(s) 722
XceI RCATGY 1 cut(s) 887
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 4 cut(s) 213, 237, 723, 1116
Zsp2I ATGCAT 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.