Rroxscaffold_3G00257010

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013870
Physical Location & Seq
Reverse (-)
51431872 .. 51434200
2329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257010.1

Sequence Viewer

Length: 864 bp
ATGGGTGCTGGCTTCTCGTTCTTTAAGGTGGACCCAGTTGGGTCTTTGCCTCTGCAATCAAAGCCGGGTCTTGGGGATTTGCCGGAGAGCTGTGCGGCTGAGATTATGAGGTACTTGGACCCGCCGGAGATTTGCAAGCTGGCCAAGATGAATCGGGCTTTTCGTGGGGCTTCCTGGGCCGATTTTATATGGGAGTCGAAATTGCCTAGTAATTATCAGATTATTGTGAACAAAGTGTTTGGTGATGGGTTGGGAAATTTGGGGAAAAGAGATGTGTATGAGAGGCTTTGTAGGCCTAACCCGTTTGATGGGGGAGCCAAGACAGTTTGGCTGGATAAGCGCACAGGATCTGCTTGCTTGTCCATTTCGTCAAAGGGATTAGCAATCACTGGAATCGATGATCGAAGATATTGGAAGCATATTCCTACCGAAGAATCTAGATTTAGCACTGTTGCATATCTTCAGCAAATATGGTGGTTTGAGGTAGATGGGGAGGTCGAGTTCCCATTTCCAGCCGGGAAATACAGCTTGTATTTTAGGCTGCAGCTGGGACGCTCTTCTCAGAAGAGGTTTGGTCGCCGTGTTTGCAACACGGAGCATGTCCATGGCTGGGACAGAAAACCAGTGAGGTTCCAACTATGGACTTCAGAAGGTCAGTCTGCTTCATCCGAGTGCTTTTTAACTGAACCTGGGAAATGGAATCACTACCATGCTGGGGACTTTGTCGTGGAAAACCCAAATGCAGCAACAAAGATTAAACTCTCGATGACCCAGATTGATTGTACACACACCAAAGGCGGTCTCTGTTTAGACTCTGTAGTTATATACCCTAGTGAATTTAGAGAAAGGTTGAAGCAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

287

Amino Acids

32.61

Weight (kDa)

8.83

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 118 - 277 1.3e-39 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12710 AT1G12710 AT1G63090
fragaria_vesca FvH4_5g01540
malus_domestica MD04G1067200.v1.1 MD06G1073200.v1.1
prunus_persica Prupe.5G091600_v2.0.a1
pyrus_communis pycom04g06030
rosa_chinensis RchiOBHm_Chr7g0199151
rosa_laevigata RLG00000003810
rosa_multiflora Rmu_sc0006266.1_g000060
rosa_roxburghii Rroxscaffold_3G00257010
rosa_rugosa Rorug07G0053000
rosa_samantha Rh7BG180200 Rh7CG185800 Rh7DG179600
rosa_wichuraiana Rw7G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 95, 122, 798
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 2 cut(s) 256, 836
AcuI CTGAAG 2 cut(s) 446, 630
AfaI GTAC 2 cut(s) 113, 784
AfiI CCNNNNNNNGG 4 cut(s) 71, 308, 610, 715
AgsI TTSAA 1 cut(s) 853
AjnI CCWGG 2 cut(s) 173, 688
AloI GAACNNNNNNTCC 2 cut(s) 485, 517
AluBI AGCT 4 cut(s) 90, 139, 528, 547
AluI AGCT 4 cut(s) 90, 139, 528, 547
Alw26I GTCTC 1 cut(s) 806
AlwI GGATC 1 cut(s) 355
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 3 cut(s) 141, 177, 293
ApeKI GCWGC 3 cut(s) 541, 544, 743
ApoI RAATTY 2 cut(s) 256, 836
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 3 cut(s) 31, 118, 177
AsuC2I CCSGG 2 cut(s) 66, 517
AsuHPI GGTGA 1 cut(s) 254
AvaII GGWCC 2 cut(s) 31, 118
BalI TGGCCA 1 cut(s) 143
BbvI GCAGC 3 cut(s) 528, 556, 755
BccI CCATC 3 cut(s) 239, 302, 482
BceAI ACGGC 1 cut(s) 564
BciT130I CCWGG 2 cut(s) 175, 690
BcnI CCSGG 2 cut(s) 66, 517
BcoDI GTCTC 1 cut(s) 806
BfaI CTAG 3 cut(s) 207, 438, 831
BfmI CTRYAG 2 cut(s) 542, 816
BisI GCNGC 4 cut(s) 96, 542, 545, 744
BlsI GCNGC 4 cut(s) 97, 543, 546, 745
Bme1390I CCNGG 4 cut(s) 66, 175, 517, 690
Bme18I GGWCC 2 cut(s) 31, 118
BmgT120I GGNCC 3 cut(s) 31, 118, 177
BmiI GGNNCC 4 cut(s) 33, 120, 316, 632
BmrFI CCNGG 4 cut(s) 66, 175, 517, 690
BmrI ACTGGG 1 cut(s) 29
BmuI ACTGGG 1 cut(s) 29
BpuMI CCSGG 2 cut(s) 66, 517
Bsa29I ATCGAT 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 806
BsaJI CCNNGG 3 cut(s) 174, 604, 689
BsaXI ACNNNNNCTCC 2 cut(s) 485, 515
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 308, 610, 715
Bse1I ACTGG 3 cut(s) 35, 394, 623
BseBI CCWGG 2 cut(s) 175, 690
BseCI ATCGAT 1 cut(s) 396
BseDI CCNNGG 3 cut(s) 174, 604, 689
BseGI GGATG 1 cut(s) 665
BseLI CCNNNNNNNGG 4 cut(s) 71, 308, 610, 715
BseMII CTCAG 2 cut(s) 90, 575
BseNI ACTGG 3 cut(s) 35, 394, 623
BseXI GCAGC 3 cut(s) 528, 556, 755
BseYI CCCAGC 3 cut(s) 547, 609, 713
BshFI GGCC 3 cut(s) 143, 179, 295
BshVI ATCGAT 1 cut(s) 396
BsiSI CCGG 4 cut(s) 65, 83, 125, 516
BslFI GGGAC 3 cut(s) 564, 626, 731
BslI CCNNNNNNNGG 4 cut(s) 71, 308, 610, 715
BsmAI GTCTC 1 cut(s) 806
BsmFI GGGAC 3 cut(s) 564, 626, 731
BsnI GGCC 3 cut(s) 143, 179, 295
Bso31I GGTCTC 1 cut(s) 806
Bsp1407I TGTACA 1 cut(s) 782
Bsp143I GATC 2 cut(s) 347, 400
Bsp19I CCATGG 1 cut(s) 604
BspACI CCGC 3 cut(s) 95, 122, 798
BspANI GGCC 3 cut(s) 143, 179, 295
BspCNI CTCAG 2 cut(s) 91, 574
BspDI ATCGAT 1 cut(s) 396
BspLI GGNNCC 4 cut(s) 33, 120, 316, 632
BspMAI CTGCAG 1 cut(s) 546
BspPI GGATC 1 cut(s) 355
BspQI GCTCTTC 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 806
BsrGI TGTACA 1 cut(s) 782
BsrI ACTGG 3 cut(s) 35, 394, 623
BssECI CCNNGG 3 cut(s) 174, 604, 689
BssMI GATC 2 cut(s) 347, 400
BssT1I CCWWGG 1 cut(s) 604
Bst2UI CCWGG 2 cut(s) 175, 690
Bst4CI ACNGT 2 cut(s) 325, 451
Bst6I CTCTTC 2 cut(s) 560, 562
BstAUI TGTACA 1 cut(s) 782
BstC8I GCNNGC 4 cut(s) 10, 137, 141, 355
BstDEI CTNAG 2 cut(s) 99, 561
BstDSI CCRYGG 1 cut(s) 604
BstF5I GGATG 1 cut(s) 665
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 2 cut(s) 350, 403
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 2 cut(s) 347, 400
BstMWI GCNNNNNNNGC 5 cut(s) 61, 176, 292, 337, 585
BstNI CCWGG 2 cut(s) 175, 690
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 4 cut(s) 64, 173, 515, 688
BstSFI CTRYAG 2 cut(s) 542, 816
BstV1I GCAGC 3 cut(s) 528, 556, 755
BstX2I RGATCY 1 cut(s) 347
BstYI RGATCY 1 cut(s) 347
Bsu15I ATCGAT 1 cut(s) 396
BsuRI GGCC 3 cut(s) 143, 179, 295
BsuTUI ATCGAT 1 cut(s) 396
BtgI CCRYGG 1 cut(s) 604
BtsCI GGATG 1 cut(s) 665
BtsIMutI CAGTG 3 cut(s) 387, 447, 630
Cac8I GCNNGC 4 cut(s) 10, 137, 141, 355
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 1 cut(s) 342
Cfr13I GGNCC 3 cut(s) 31, 118, 177
ClaI ATCGAT 1 cut(s) 396
CseI GACGC 1 cut(s) 561
Csp6I GTAC 2 cut(s) 112, 783
CspCI CAANNNNNGTGG 2 cut(s) 455, 490
CviAII CATG 3 cut(s) 599, 605, 710
CviQI GTAC 2 cut(s) 112, 783
DdeI CTNAG 2 cut(s) 99, 561
DpnI GATC 2 cut(s) 349, 402
DpnII GATC 2 cut(s) 347, 400
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 2 cut(s) 560, 562
EarI CTCTTC 2 cut(s) 560, 562
Eco130I CCWWGG 1 cut(s) 604
Eco147I AGGCCT 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 806
Eco47I GGWCC 2 cut(s) 31, 118
Eco57I CTGAAG 2 cut(s) 446, 630
EcoRII CCWGG 2 cut(s) 173, 688
EcoT14I CCWWGG 1 cut(s) 604
ErhI CCWWGG 1 cut(s) 604
FaeI CATG 3 cut(s) 602, 608, 713
FaqI GGGAC 3 cut(s) 564, 626, 731
FatI CATG 3 cut(s) 598, 604, 709
FauI CCCGC 1 cut(s) 129
Fnu4HI GCNGC 4 cut(s) 96, 542, 545, 744
FokI GGATG 1 cut(s) 652
Fsp4HI GCNGC 4 cut(s) 96, 542, 545, 744
FspBI CTAG 3 cut(s) 207, 438, 831
GlaI GCGC 1 cut(s) 341
GluI GCNGC 4 cut(s) 96, 542, 545, 744
GsaI CCCAGC 3 cut(s) 551, 613, 717
HaeIII GGCC 3 cut(s) 143, 179, 295
HapII CCGG 4 cut(s) 65, 83, 125, 516
HgaI GACGC 1 cut(s) 561
HhaI GCGC 1 cut(s) 342
Hin1II CATG 3 cut(s) 602, 608, 713
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HinfI GANTC 6 cut(s) 151, 194, 393, 434, 700, 812
HpaII CCGG 4 cut(s) 65, 83, 125, 516
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 3 cut(s) 31, 229, 785
Hpy188I TCNGA 4 cut(s) 219, 564, 649, 670
Hpy188III TCNNGA 2 cut(s) 438, 763
Hpy8I GTNNAC 3 cut(s) 31, 229, 785
HpyAV CCTTC 1 cut(s) 644
HpyCH4III ACNGT 2 cut(s) 325, 451
HpyCH4V TGCA 6 cut(s) 55, 135, 455, 544, 588, 743
HpyF10VI GCNNNNNNNGC 5 cut(s) 61, 176, 292, 337, 585
HpyF3I CTNAG 2 cut(s) 99, 561
Hsp92II CATG 3 cut(s) 602, 608, 713
HspAI GCGC 1 cut(s) 340
Kzo9I GATC 2 cut(s) 347, 400
LguI GCTCTTC 1 cut(s) 562
LmnI GCTCC 2 cut(s) 314, 595
Lsp1109I GCAGC 3 cut(s) 528, 556, 755
MaeI CTAG 3 cut(s) 207, 438, 831
MalI GATC 2 cut(s) 349, 402
MboI GATC 2 cut(s) 347, 400
MboII GAAGA 5 cut(s) 417, 443, 452, 549, 577
MflI RGATCY 1 cut(s) 347
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 4 cut(s) 200, 211, 256, 836
MluNI TGGCCA 1 cut(s) 143
MlyI GAGTC 2 cut(s) 203, 806
MmeI TCCRAC 1 cut(s) 658
MnlI CCTC 7 cut(s) 60, 102, 276, 475, 487, 561, 621
Mox20I TGGCCA 1 cut(s) 143
MscI TGGCCA 1 cut(s) 143
MseI TTAA 3 cut(s) 24, 680, 756
MslI CAYNNNNRTG 3 cut(s) 603, 670, 708
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 547
MspI CCGG 4 cut(s) 65, 83, 125, 516
MspR9I CCNGG 4 cut(s) 66, 175, 517, 690
MvaI CCWGG 2 cut(s) 175, 690
MwoI GCNNNNNNNGC 5 cut(s) 61, 176, 292, 337, 585
NciI CCSGG 2 cut(s) 66, 517
NcoI CCATGG 1 cut(s) 604
NdeII GATC 2 cut(s) 347, 400
NlaIII CATG 3 cut(s) 602, 608, 713
NlaIV GGNNCC 4 cut(s) 33, 120, 316, 632
NspI RCATGY 1 cut(s) 602
PceI AGGCCT 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 562
PcsI WCGNNNNNNNCGW 1 cut(s) 160
PfeI GAWTC 4 cut(s) 151, 393, 434, 700
PflFI GACNNNGTC 1 cut(s) 722
PkrI GCNGC 4 cut(s) 97, 543, 546, 745
PleI GAGTC 2 cut(s) 202, 806
PpsI GAGTC 2 cut(s) 202, 806
Psp6I CCWGG 2 cut(s) 173, 688
PspFI CCCAGC 3 cut(s) 547, 609, 713
PspGI CCWGG 2 cut(s) 173, 688
PspN4I GGNNCC 4 cut(s) 33, 120, 316, 632
PspPI GGNCC 3 cut(s) 31, 118, 177
PstI CTGCAG 1 cut(s) 546
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 347
PsyI GACNNNGTC 1 cut(s) 722
PvuII CAGCTG 1 cut(s) 547
RsaI GTAC 2 cut(s) 113, 784
RsaNI GTAC 2 cut(s) 112, 783
RseI CAYNNNNRTG 3 cut(s) 603, 670, 708
SapI GCTCTTC 1 cut(s) 562
SaqAI TTAA 3 cut(s) 24, 680, 756
SatI GCNGC 4 cut(s) 96, 542, 545, 744
Sau3AI GATC 2 cut(s) 347, 400
Sau96I GGNCC 3 cut(s) 31, 118, 177
SchI GAGTC 2 cut(s) 203, 806
ScrFI CCNGG 4 cut(s) 66, 175, 517, 690
SfcI CTRYAG 2 cut(s) 542, 816
SinI GGWCC 2 cut(s) 31, 118
SmiMI CAYNNNNRTG 3 cut(s) 603, 670, 708
Sse9I AATT 4 cut(s) 200, 211, 256, 836
SseBI AGGCCT 1 cut(s) 295
SsiI CCGC 3 cut(s) 95, 122, 798
SspMI CTAG 3 cut(s) 207, 438, 831
StuI AGGCCT 1 cut(s) 295
StyD4I CCNGG 4 cut(s) 64, 173, 515, 688
StyI CCWWGG 1 cut(s) 604
TaaI ACNGT 2 cut(s) 325, 451
TaqI TCGA 5 cut(s) 197, 396, 403, 498, 764
TasI AATT 4 cut(s) 200, 211, 256, 836
TatI WGTACW 1 cut(s) 782
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 4 cut(s) 151, 393, 434, 700
Tru1I TTAA 3 cut(s) 24, 680, 756
Tru9I TTAA 3 cut(s) 24, 680, 756
TscAI CASTG 3 cut(s) 394, 454, 630
TseI GCWGC 3 cut(s) 541, 544, 743
TspDTI ATGAA 2 cut(s) 164, 654
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 3 cut(s) 394, 454, 630
Tth111I GACNNNGTC 1 cut(s) 722
VpaK11BI GGWCC 2 cut(s) 31, 118
XapI RAATTY 2 cut(s) 256, 836
XbaI TCTAGA 1 cut(s) 437
XceI RCATGY 1 cut(s) 602
XspI CTAG 3 cut(s) 207, 438, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.