MD06G1087400.v1.1

Sin3 binding region of histone deacetylase complex subunit SAP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010563
Physical Location & Seq
Reverse (-)
21397030 .. 21401283
4254 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1087400.v1.1.491

Sequence Viewer

Length: 678 bp
ATGCTGGAGCCAGAGTTCTGTGGTTCTCGGATGCTATCGCCTTTTCGGGACGAAAGCGGAGATGAGGAACTTTCAGTTCTTCCCAGGCACACCAAGGTTATTGTCACTGGAAACAACAGAACAAAGTCTGTTTTGGTGGGTCTTCAAGGCGTGGTCAAGAAAGCTGTTGGGCTTGGGGGTTGGCACTGGCTGGTTCTGAAAAATGGAGTTGAAGTTAAGCTGCAAAGGAATGCATTGAGTGTGCTGGAACATCCTACAGGGAATGAGGAAGATAGCAATCTTGATTACTCTAGCGGCAGCTCTGACCATGGTGATAAGGAGAATGATTTCTCTAGATGCTTTGAGTTCCATAAGCATAGTAAACCAAGAGTTCGATTTTCAAGGCCATATGTTCCACCCTCAGCAACAAAGTCAGTAAATCGTGGAAGTTATAGAGAAGTTCAATACATCCAAGCACCCCAATCGAGAGTAAACTTGGGAAAACTAGGAACAAGCTCATTGAGGAGGTATTGCAGACACTTCAATCTTTTAAGCGGCAATTCTAATCCAACAAGGGAACAATTGATCAATGCTGCACAACGACATTTTACATCGCAGCAGATGCCGTTGGATGAGGTATCGGTGGTCACTGAATTTGTGGATGCTGCCAGGAGTCGGAAGTGGAAACAAGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAP30_Sin3_bdg

Protein Analysis

226

Amino Acids

25.41

Weight (kDa)

9.42

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP30_Sin3_bdg PF13867 162 - 217 1.7e-17 Sin3 binding region of histone deacetylase complex subunit SAP30
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 57, 294, 534
AcsI RAATTY 1 cut(s) 632
AfiI CCNNNNNNNGG 1 cut(s) 654
AgsI TTSAA 5 cut(s) 146, 212, 381, 443, 523
AjnI CCWGG 2 cut(s) 83, 647
AloI GAACNNNNNNTCC 1 cut(s) 31
AluBI AGCT 4 cut(s) 164, 220, 300, 495
AluI AGCT 4 cut(s) 164, 220, 300, 495
AoxI GGCC 1 cut(s) 383
ApeKI GCWGC 5 cut(s) 220, 297, 572, 595, 644
ApoI RAATTY 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 134
BbvCI CCTCAGC 1 cut(s) 400
BbvI GCAGC 5 cut(s) 207, 309, 559, 607, 631
BceAI ACGGC 1 cut(s) 589
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciT130I CCWGG 2 cut(s) 85, 649
BclI TGATCA 1 cut(s) 564
BfaI CTAG 3 cut(s) 291, 333, 485
BfmI CTRYAG 1 cut(s) 255
BisI GCNGC 7 cut(s) 221, 295, 298, 535, 573, 596, 645
BlsI GCNGC 7 cut(s) 222, 296, 299, 536, 574, 597, 646
Bme1390I CCNGG 2 cut(s) 85, 649
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 85, 649
BmsI GCATC 4 cut(s) 21, 326, 591, 631
BpiI GAAGAC 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 400
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 3 cut(s) 83, 93, 307
BsaXI ACNNNNNCTCC 3 cut(s) 29, 198, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 654
Bse1I ACTGG 2 cut(s) 112, 191
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 2 cut(s) 85, 649
BseDI CCNNGG 3 cut(s) 83, 93, 307
BseGI GGATG 5 cut(s) 36, 250, 447, 616, 646
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 654
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 2 cut(s) 112, 191
BseRI GAGGAG 1 cut(s) 517
BseXI GCAGC 5 cut(s) 207, 309, 559, 607, 631
BsgI GTGCAG 1 cut(s) 558
BshFI GGCC 1 cut(s) 385
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 654
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 1 cut(s) 385
Bsp143I GATC 1 cut(s) 564
Bsp19I CCATGG 1 cut(s) 307
BspACI CCGC 3 cut(s) 57, 294, 534
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 1 cut(s) 413
BspLI GGNNCC 1 cut(s) 9
BsrI ACTGG 2 cut(s) 112, 191
BssECI CCNNGG 3 cut(s) 83, 93, 307
BssMI GATC 1 cut(s) 564
BssT1I CCWWGG 2 cut(s) 93, 307
Bst2UI CCWGG 2 cut(s) 85, 649
BstAPI GCANNNNNTGC 1 cut(s) 601
BstDEI CTNAG 1 cut(s) 400
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 5 cut(s) 36, 250, 447, 616, 646
BstKTI GATC 1 cut(s) 567
BstMBI GATC 1 cut(s) 564
BstMWI GCNNNNNNNGC 1 cut(s) 601
BstNI CCWGG 2 cut(s) 85, 649
BstSCI CCNGG 2 cut(s) 83, 647
BstSFI CTRYAG 1 cut(s) 255
BstV1I GCAGC 5 cut(s) 207, 309, 559, 607, 631
BstV2I GAAGAC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 385
BtgI CCRYGG 1 cut(s) 307
BtgZI GCGATG 1 cut(s) 576
BtsCI GGATG 5 cut(s) 36, 250, 447, 616, 646
BtsIMutI CAGTG 3 cut(s) 105, 184, 627
CviAII CATG 1 cut(s) 308
CviJI RGCY 8 cut(s) 10, 164, 172, 190, 220, 300, 385, 495
CviKI_1 RGCY 8 cut(s) 10, 164, 172, 190, 220, 300, 385, 495
DdeI CTNAG 1 cut(s) 400
DpnI GATC 1 cut(s) 566
DpnII GATC 1 cut(s) 564
Eco130I CCWWGG 2 cut(s) 93, 307
EcoRII CCWGG 2 cut(s) 83, 647
EcoT14I CCWWGG 2 cut(s) 93, 307
EcoT22I ATGCAT 1 cut(s) 235
ErhI CCWWGG 2 cut(s) 93, 307
FaeI CATG 1 cut(s) 311
FaiI YATR 6 cut(s) 309, 351, 357, 388, 390, 432
FaqI GGGAC 1 cut(s) 62
FatI CATG 1 cut(s) 307
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 7 cut(s) 221, 295, 298, 535, 573, 596, 645
FokI GGATG 5 cut(s) 43, 237, 434, 623, 653
Fsp4HI GCNGC 7 cut(s) 221, 295, 298, 535, 573, 596, 645
FspBI CTAG 3 cut(s) 291, 333, 485
GluI GCNGC 7 cut(s) 221, 295, 298, 535, 573, 596, 645
GsuI CTGGAG 1 cut(s) 26
HaeIII GGCC 1 cut(s) 385
Hin1II CATG 1 cut(s) 311
HinfI GANTC 1 cut(s) 652
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 362, 472
Hpy188I TCNGA 4 cut(s) 30, 198, 304, 657
Hpy188III TCNNGA 5 cut(s) 47, 157, 281, 333, 465
Hpy8I GTNNAC 2 cut(s) 362, 472
HpyCH4V TGCA 4 cut(s) 223, 233, 513, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 601
HpyF3I CTNAG 1 cut(s) 400
Hsp92II CATG 1 cut(s) 311
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 1 cut(s) 564
LmnI GCTCC 1 cut(s) 7
Lsp1109I GCAGC 5 cut(s) 207, 309, 559, 607, 631
LweI GCATC 4 cut(s) 21, 326, 591, 631
MaeI CTAG 3 cut(s) 291, 333, 485
MaeIII GTNAC 2 cut(s) 103, 625
MalI GATC 1 cut(s) 566
MboI GATC 1 cut(s) 564
MboII GAAGA 3 cut(s) 71, 134, 281
MfeI CAATTG 1 cut(s) 560
MluCI AATT 3 cut(s) 538, 560, 632
MlyI GAGTC 1 cut(s) 661
MmeI TCCRAC 3 cut(s) 572, 588, 635
MnlI CCTC 6 cut(s) 58, 259, 409, 495, 498, 607
Mph1103I ATGCAT 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 326
MseI TTAA 2 cut(s) 216, 530
MspR9I CCNGG 2 cut(s) 85, 649
MunI CAATTG 1 cut(s) 560
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 2 cut(s) 85, 649
MwoI GCNNNNNNNGC 1 cut(s) 601
NcoI CCATGG 1 cut(s) 307
NdeI CATATG 1 cut(s) 388
NdeII GATC 1 cut(s) 564
NlaIII CATG 1 cut(s) 311
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 2 cut(s) 103, 625
NsiI ATGCAT 1 cut(s) 235
PctI GAATGC 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 326
PkrI GCNGC 7 cut(s) 222, 296, 299, 536, 574, 597, 646
PleI GAGTC 1 cut(s) 660
PpsI GAGTC 1 cut(s) 660
Psp6I CCWGG 2 cut(s) 83, 647
PspGI CCWGG 2 cut(s) 83, 647
PspN4I GGNNCC 1 cut(s) 9
SaqAI TTAA 2 cut(s) 216, 530
SatI GCNGC 7 cut(s) 221, 295, 298, 535, 573, 596, 645
Sau3AI GATC 1 cut(s) 564
SchI GAGTC 1 cut(s) 661
ScrFI CCNGG 2 cut(s) 85, 649
SetI ASST 7 cut(s) 99, 166, 222, 302, 497, 509, 618
SfaNI GCATC 4 cut(s) 21, 326, 591, 631
SfcI CTRYAG 1 cut(s) 255
Sse9I AATT 3 cut(s) 538, 560, 632
SsiI CCGC 3 cut(s) 57, 294, 534
SspMI CTAG 3 cut(s) 291, 333, 485
StyD4I CCNGG 2 cut(s) 83, 647
StyI CCWWGG 2 cut(s) 93, 307
TaqI TCGA 2 cut(s) 373, 464
TasI AATT 3 cut(s) 538, 560, 632
TauI GCSGC 2 cut(s) 297, 537
Tru1I TTAA 2 cut(s) 216, 530
Tru9I TTAA 2 cut(s) 216, 530
TscAI CASTG 3 cut(s) 112, 191, 634
TseFI GTSAC 2 cut(s) 103, 625
TseI GCWGC 5 cut(s) 220, 297, 572, 595, 644
Tsp45I GTSAC 2 cut(s) 103, 625
TspRI CASTG 3 cut(s) 112, 191, 634
XapI RAATTY 1 cut(s) 632
XbaI TCTAGA 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 3 cut(s) 291, 333, 485
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.