MD14G1108300.v1.1

Sin3 binding region of histone deacetylase complex subunit SAP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010563
Physical Location & Seq
Reverse (-)
16879928 .. 16883869
3942 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1108300.v1.1.491

Sequence Viewer

Length: 687 bp
ATGCTGGAGGCAGAGCTCTGTGGTTCTCGGATTCTATCGCCTTTTCGCGAAGAAAGCGGGGATGAGGAACTTTCAGTTCTTCCCAGGCACACAAAGGTTATTGTCACTGGAAACAATAGAACAAAATCTATTTTGGTGGGTCTTCAAGGCGTGGTCAAGAAAGCTGTTGGCCTTGGGGGTTGGCACTGGCTGGTTCTGAAAAATGGGGTTGAAGTTAAGCTGCAAAGGAATGCATTGAGTGTGCTCGAACATCCTACAGGGAATGAGGAAGATTCCAATCTTGAGTACTCTAGCAGCAGCTCTGACCACGGTGATAAGGAAAATGATTTCTCTAGATGTTTTGAGTTCCATAAGCATAGTAAACCAAGAGTTCGATTTTCAAGGCCATATGTTCCATCCTCAGCAACCAAGTCAATGAATCGTGGCAGTTATAGAGAAGTTCAATACATCCAAGCACCCCAGTCGAGAGTAAACTTGGGAAAACTAGGAACAAGCTCGTTGAGGAGGTATTGCAGACACTTCAATCTTTTAAGCGGGAATTCTAATCCAACAAGGGAACAGTTGATCAATGCTGCACAACGACATTTTACCTCGCAGCAGATGCCGTTGGATGAGGTATCTGTGGTCACTGAGTTCGTCGATGCAGGCAGGAGTAGGAAGTGGAAACAAGCAGAGCGACAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAP30_Sin3_bdg

Protein Analysis

229

Amino Acids

25.83

Weight (kDa)

9.54

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP30_Sin3_bdg PF13867 162 - 215 6.4e-17 Sin3 binding region of histone deacetylase complex subunit SAP30
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 48
AciI CCGC 2 cut(s) 57, 534
AcsI RAATTY 1 cut(s) 538
AfaI GTAC 1 cut(s) 287
AgsI TTSAA 5 cut(s) 146, 212, 381, 443, 523
AjnI CCWGG 1 cut(s) 83
AluBI AGCT 5 cut(s) 16, 164, 220, 300, 495
AluI AGCT 5 cut(s) 16, 164, 220, 300, 495
Alw21I GWGCWC 2 cut(s) 18, 246
AoxI GGCC 2 cut(s) 169, 383
ApeKI GCWGC 5 cut(s) 220, 294, 297, 572, 595
ApoI RAATTY 1 cut(s) 538
AsuHPI GGTGA 1 cut(s) 323
BanII GRGCYC 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 2 cut(s) 18, 246
BbvCI CCTCAGC 1 cut(s) 400
BbvI GCAGC 5 cut(s) 207, 306, 309, 559, 607
BccI CCATC 1 cut(s) 403
BceAI ACGGC 1 cut(s) 589
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciT130I CCWGG 1 cut(s) 85
BclI TGATCA 1 cut(s) 564
BfaI CTAG 3 cut(s) 291, 333, 485
BfmI CTRYAG 1 cut(s) 255
BisI GCNGC 5 cut(s) 221, 295, 298, 573, 596
BlsI GCNGC 5 cut(s) 222, 296, 299, 574, 597
BmcAI AGTACT 1 cut(s) 287
Bme1390I CCNGG 1 cut(s) 85
BmrFI CCNGG 1 cut(s) 85
BmrI ACTGGG 1 cut(s) 454
BmsI GCATC 2 cut(s) 591, 631
BmuI ACTGGG 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 400
BpuEI CTTGAG 1 cut(s) 302
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 3 cut(s) 83, 172, 307
Bse1I ACTGG 3 cut(s) 112, 191, 460
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 3 cut(s) 83, 172, 307
BseGI GGATG 5 cut(s) 67, 250, 395, 447, 616
BseJI GATNNNNATC 1 cut(s) 276
BseMII CTCAG 2 cut(s) 414, 621
BseNI ACTGG 3 cut(s) 112, 191, 460
BseRI GAGGAG 1 cut(s) 517
BseXI GCAGC 5 cut(s) 207, 306, 309, 559, 607
BsgI GTGCAG 1 cut(s) 558
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 2 cut(s) 171, 385
BsiHKAI GWGCWC 2 cut(s) 18, 246
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 2 cut(s) 171, 385
Bsp1286I GDGCHC 2 cut(s) 18, 246
Bsp143I GATC 1 cut(s) 564
Bsp68I TCGCGA 1 cut(s) 48
BspACI CCGC 2 cut(s) 57, 534
BspANI GGCC 2 cut(s) 171, 385
BspCNI CTCAG 2 cut(s) 413, 622
BspFNI CGCG 1 cut(s) 48
BsrI ACTGG 3 cut(s) 112, 191, 460
BssECI CCNNGG 3 cut(s) 83, 172, 307
BssMI GATC 1 cut(s) 564
BssT1I CCWWGG 1 cut(s) 172
Bst2UI CCWGG 1 cut(s) 85
Bst4CI ACNGT 2 cut(s) 311, 561
BstAPI GCANNNNNTGC 1 cut(s) 601
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 2 cut(s) 400, 630
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 5 cut(s) 67, 250, 395, 447, 616
BstFNI CGCG 1 cut(s) 48
BstKTI GATC 1 cut(s) 567
BstMBI GATC 1 cut(s) 564
BstMWI GCNNNNNNNGC 2 cut(s) 54, 601
BstNI CCWGG 1 cut(s) 85
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 1 cut(s) 255
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 5 cut(s) 207, 306, 309, 559, 607
BstV2I GAAGAC 1 cut(s) 134
BsuRI GGCC 2 cut(s) 171, 385
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 5 cut(s) 67, 250, 395, 447, 616
BtsIMutI CAGTG 3 cut(s) 105, 184, 627
BtuMI TCGCGA 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 646
Csp6I GTAC 1 cut(s) 286
CviJI RGCY 8 cut(s) 16, 164, 171, 190, 220, 300, 385, 495
CviKI_1 RGCY 8 cut(s) 16, 164, 171, 190, 220, 300, 385, 495
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 2 cut(s) 400, 630
DpnI GATC 1 cut(s) 566
DpnII GATC 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 83
EcoT14I CCWWGG 1 cut(s) 172
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 172
FaiI YATR 5 cut(s) 351, 357, 388, 390, 432
FauI CCCGC 2 cut(s) 50, 527
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 5 cut(s) 221, 295, 298, 573, 596
FokI GGATG 5 cut(s) 74, 237, 382, 434, 623
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 5 cut(s) 221, 295, 298, 573, 596
FspBI CTAG 3 cut(s) 291, 333, 485
GluI GCNGC 5 cut(s) 221, 295, 298, 573, 596
GsuI CTGGAG 1 cut(s) 26
HaeIII GGCC 2 cut(s) 171, 385
HinfI GANTC 3 cut(s) 31, 272, 418
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 362, 472
Hpy188I TCNGA 3 cut(s) 30, 198, 304
Hpy188III TCNNGA 5 cut(s) 47, 157, 281, 333, 465
Hpy8I GTNNAC 2 cut(s) 362, 472
Hpy99I CGWCG 1 cut(s) 641
HpyCH4III ACNGT 2 cut(s) 311, 561
HpyCH4V TGCA 5 cut(s) 223, 233, 513, 575, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 601
HpyF3I CTNAG 2 cut(s) 400, 630
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 1 cut(s) 564
LpnPI CCDG 9 cut(s) 70, 93, 97, 172, 176, 243, 473, 630, 634
Lsp1109I GCAGC 5 cut(s) 207, 306, 309, 559, 607
LweI GCATC 2 cut(s) 591, 631
MaeI CTAG 3 cut(s) 291, 333, 485
MaeIII GTNAC 2 cut(s) 103, 625
MalI GATC 1 cut(s) 566
MboI GATC 1 cut(s) 564
MboII GAAGA 4 cut(s) 62, 71, 134, 281
MhlI GDGCHC 2 cut(s) 18, 246
MluCI AATT 1 cut(s) 538
MmeI TCCRAC 2 cut(s) 572, 588
MnlI CCTC 7 cut(s) 58, 259, 409, 495, 498, 601, 607
Mph1103I ATGCAT 1 cut(s) 235
MseI TTAA 2 cut(s) 216, 530
MspR9I CCNGG 1 cut(s) 85
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 1 cut(s) 85
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 2 cut(s) 54, 601
NdeI CATATG 1 cut(s) 388
NdeII GATC 1 cut(s) 564
NmuCI GTSAC 2 cut(s) 103, 625
NruI TCGCGA 1 cut(s) 48
NsiI ATGCAT 1 cut(s) 235
PctI GAATGC 1 cut(s) 235
PfeI GAWTC 3 cut(s) 31, 272, 418
PkrI GCNGC 5 cut(s) 222, 296, 299, 574, 597
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 83
PspGI CCWGG 1 cut(s) 83
RruI TCGCGA 1 cut(s) 48
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SacI GAGCTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 216, 530
SatI GCNGC 5 cut(s) 221, 295, 298, 573, 596
Sau3AI GATC 1 cut(s) 564
ScaI AGTACT 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 85
SduI GDGCHC 2 cut(s) 18, 246
SetI ASST 9 cut(s) 18, 99, 166, 222, 302, 497, 509, 593, 618
SfaNI GCATC 2 cut(s) 591, 631
SfcI CTRYAG 1 cut(s) 255
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 1 cut(s) 538
SsiI CCGC 2 cut(s) 57, 534
SspMI CTAG 3 cut(s) 291, 333, 485
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 2 cut(s) 311, 561
TaqI TCGA 4 cut(s) 246, 373, 464, 639
TasI AATT 1 cut(s) 538
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 3 cut(s) 31, 272, 418
Tru1I TTAA 2 cut(s) 216, 530
Tru9I TTAA 2 cut(s) 216, 530
TscAI CASTG 3 cut(s) 112, 191, 634
TseFI GTSAC 2 cut(s) 103, 625
TseI GCWGC 5 cut(s) 220, 294, 297, 572, 595
Tsp45I GTSAC 2 cut(s) 103, 625
TspDTI ATGAA 1 cut(s) 431
TspRI CASTG 3 cut(s) 112, 191, 634
XapI RAATTY 1 cut(s) 538
XbaI TCTAGA 1 cut(s) 332
XspI CTAG 3 cut(s) 291, 333, 485
ZrmI AGTACT 1 cut(s) 287
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.