RchiOBHm_Chr7g0197141

Sin3 binding region of histone deacetylase complex subunit SAP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010563
Physical Location & Seq
Forward (+)
15057855 .. 15061471
3617 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17635

Sequence Viewer

Length: 702 bp
ATGCTAGAGCCTGAGCTGTGTGCTTCTAGGGTGCTGTCACCTTCTCATGACGAAAGTGGGGAGGATGAGCTTTCAGTTCTTCCCAGGCACACTAAGGTAATTGTTACTGGGAACAACAGAACAAAGTCTGTGTTGGTGGGTCTACAGGGAGTGGTCAAGAAAGCGGTTGGGCTTGGTGGTTGGCACTGGCTGGTTCTGAAAAATGGCGTTGAAGTCAAGCTTCAAAGGAATGCATTGAGTGTGCTGGAACATCCTACAGGGAATGAGGAAGATTTCAACAATGATTATGATAACTCTAGCAGTAGCTCTGAACTGGGTGTGAAGGAGAATGATTTCACTAGGAGTCTTGAGTTCCACAGACATGATAAACCAAGATTTCGGTATCCAAAATCATATGTTCCATCCACCAAGTCGATTTATCGTGGCAGTTATCAAGAATTTCAGTCCATCCACAAACCTCAGCTGAGAGTAAACATGGCAAAACTAGGAACAAGCACTTTGCGGAGGTACTGCAGGCACTTCAACCTTGTAAGCAGCTATCCTAATCCAACGAGGGAGCAACTGATTAACGTTGTGCAACGACATTTTACATCACAGCTGATGCGATTGGATGAGGTATCAGTGATCACTGAATTTGTCAATGCTGCAAGGAGGCGCAAATCTGCCGAGTTGGCTAGGAAACGTACAGAACGAGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAP30_Sin3_bdg

Protein Analysis

233

Amino Acids

26.78

Weight (kDa)

9.63

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP30_Sin3_bdg PF13867 162 - 217 1e-18 Sin3 binding region of histone deacetylase complex subunit SAP30
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 142
AciI CCGC 2 cut(s) 164, 502
AclI AACGTT 1 cut(s) 570
AcsI RAATTY 2 cut(s) 437, 632
AfaI GTAC 2 cut(s) 509, 685
AgsI TTSAA 4 cut(s) 212, 224, 277, 523
AjnI CCWGG 1 cut(s) 83
AluBI AGCT 7 cut(s) 16, 70, 220, 306, 463, 537, 598
AluI AGCT 7 cut(s) 16, 70, 220, 306, 463, 537, 598
ApeKI GCWGC 2 cut(s) 534, 644
ApoI RAATTY 2 cut(s) 437, 632
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 1 cut(s) 657
AsuHPI GGTGA 1 cut(s) 30
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 2 cut(s) 546, 631
BccI CCATC 2 cut(s) 409, 455
BciT130I CCWGG 1 cut(s) 85
BciVI GTATCC 1 cut(s) 393
BclI TGATCA 1 cut(s) 624
BfaI CTAG 6 cut(s) 5, 27, 297, 339, 485, 675
BfmI CTRYAG 3 cut(s) 143, 255, 511
BfuI GTATCC 1 cut(s) 393
BglI GCCNNNNNGGC 1 cut(s) 671
BisI GCNGC 2 cut(s) 535, 645
BlsI GCNGC 2 cut(s) 536, 646
Bme1390I CCNGG 1 cut(s) 85
BmrFI CCNGG 1 cut(s) 85
BmrI ACTGGG 2 cut(s) 117, 323
BmsI GCATC 1 cut(s) 591
BmuI ACTGGG 2 cut(s) 117, 323
Bpu10I CCTNAGC 2 cut(s) 12, 459
BpuEI CTTGAG 1 cut(s) 368
BsaJI CCNNGG 1 cut(s) 83
Bse1I ACTGG 3 cut(s) 112, 191, 318
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 1 cut(s) 83
BseGI GGATG 5 cut(s) 70, 250, 401, 447, 616
BseMII CTCAG 2 cut(s) 455, 473
BseNI ACTGG 3 cut(s) 112, 191, 318
BseXI GCAGC 2 cut(s) 546, 631
BsmI GAATGC 1 cut(s) 235
Bsp143I GATC 1 cut(s) 624
BspACI CCGC 2 cut(s) 164, 502
BspCNI CTCAG 3 cut(s) 4, 456, 472
BspHI TCATGA 1 cut(s) 46
BspMAI CTGCAG 1 cut(s) 515
BsrI ACTGG 3 cut(s) 112, 191, 318
BssECI CCNNGG 1 cut(s) 83
BssMI GATC 1 cut(s) 624
Bst2UI CCWGG 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 515
BstDEI CTNAG 4 cut(s) 12, 93, 459, 464
BstF5I GGATG 5 cut(s) 70, 250, 401, 447, 616
BstHHI GCGC 1 cut(s) 657
BstKTI GATC 1 cut(s) 627
BstMBI GATC 1 cut(s) 624
BstMWI GCNNNNNNNGC 1 cut(s) 671
BstNI CCWGG 1 cut(s) 85
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 3 cut(s) 143, 255, 511
BstV1I GCAGC 2 cut(s) 546, 631
BsuI GTATCC 1 cut(s) 393
BtsCI GGATG 5 cut(s) 70, 250, 401, 447, 616
BtsIMutI CAGTG 3 cut(s) 184, 627, 627
Cac8I GCNNGC 1 cut(s) 515
CciI TCATGA 1 cut(s) 46
CfoI GCGC 1 cut(s) 657
Csp6I GTAC 2 cut(s) 508, 684
CviAII CATG 3 cut(s) 47, 362, 475
CviQI GTAC 2 cut(s) 508, 684
DdeI CTNAG 4 cut(s) 12, 93, 459, 464
DpnI GATC 1 cut(s) 626
DpnII GATC 1 cut(s) 624
EcoRII CCWGG 1 cut(s) 83
EcoT22I ATGCAT 1 cut(s) 235
FaeI CATG 3 cut(s) 50, 365, 478
FaiI YATR 6 cut(s) 48, 288, 363, 394, 396, 476
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FatI CATG 3 cut(s) 46, 361, 474
FauNDI CATATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 624
FblI GTMKAC 1 cut(s) 142
Fnu4HI GCNGC 2 cut(s) 535, 645
FokI GGATG 5 cut(s) 77, 237, 388, 434, 623
Fsp4HI GCNGC 2 cut(s) 535, 645
FspBI CTAG 6 cut(s) 5, 27, 297, 339, 485, 675
GlaI GCGC 1 cut(s) 656
GluI GCNGC 2 cut(s) 535, 645
HhaI GCGC 1 cut(s) 657
Hin1II CATG 3 cut(s) 50, 365, 478
Hin6I GCGC 1 cut(s) 655
HinP1I GCGC 1 cut(s) 655
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 1 cut(s) 343
HphI GGTGA 1 cut(s) 30
Hpy166II GTNNAC 2 cut(s) 143, 472
Hpy188I TCNGA 2 cut(s) 198, 310
Hpy188III TCNNGA 4 cut(s) 47, 157, 347, 434
Hpy8I GTNNAC 2 cut(s) 143, 472
HpyAV CCTTC 2 cut(s) 51, 316
HpyCH4IV ACGT 2 cut(s) 570, 682
HpyCH4V TGCA 4 cut(s) 233, 513, 577, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 671
HpyF3I CTNAG 4 cut(s) 12, 93, 459, 464
HpySE526I ACGT 2 cut(s) 570, 682
Hsp92II CATG 3 cut(s) 50, 365, 478
HspAI GCGC 1 cut(s) 655
Ksp22I TGATCA 1 cut(s) 624
Kzo9I GATC 1 cut(s) 624
LmnI GCTCC 1 cut(s) 556
Lsp1109I GCAGC 2 cut(s) 546, 631
LweI GCATC 1 cut(s) 591
MaeI CTAG 6 cut(s) 5, 27, 297, 339, 485, 675
MaeII ACGT 2 cut(s) 570, 682
MaeIII GTNAC 2 cut(s) 36, 103
MalI GATC 1 cut(s) 626
MboI GATC 1 cut(s) 624
MboII GAAGA 2 cut(s) 71, 281
MluCI AATT 3 cut(s) 99, 437, 632
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 1 cut(s) 572
MnlI CCTC 7 cut(s) 55, 259, 468, 498, 546, 607, 645
Mph1103I ATGCAT 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 332
MseI TTAA 1 cut(s) 567
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 2 cut(s) 463, 598
MspR9I CCNGG 1 cut(s) 85
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 1 cut(s) 85
MwoI GCNNNNNNNGC 1 cut(s) 671
NdeI CATATG 1 cut(s) 394
NdeII GATC 1 cut(s) 624
NlaIII CATG 3 cut(s) 50, 365, 478
NmeAIII GCCGAG 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 235
PagI TCATGA 1 cut(s) 46
PcsI WCGNNNNNNNCGW 1 cut(s) 688
PctI GAATGC 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 332
PkrI GCNGC 2 cut(s) 536, 646
PleI GAGTC 1 cut(s) 351
PpsI GAGTC 1 cut(s) 351
Psp1406I AACGTT 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 83
PspGI CCWGG 1 cut(s) 83
PstI CTGCAG 1 cut(s) 515
PvuII CAGCTG 2 cut(s) 463, 598
RsaI GTAC 2 cut(s) 509, 685
RsaNI GTAC 2 cut(s) 508, 684
RseI CAYNNNNRTG 1 cut(s) 360
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 2 cut(s) 535, 645
Sau3AI GATC 1 cut(s) 624
SchI GAGTC 1 cut(s) 352
ScrFI CCNGG 1 cut(s) 85
SfaNI GCATC 1 cut(s) 591
SfcI CTRYAG 3 cut(s) 143, 255, 511
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 3 cut(s) 99, 437, 632
SsiI CCGC 2 cut(s) 164, 502
SspMI CTAG 6 cut(s) 5, 27, 297, 339, 485, 675
StyD4I CCNGG 1 cut(s) 83
TaiI ACGT 2 cut(s) 573, 685
TaqI TCGA 1 cut(s) 413
TasI AATT 3 cut(s) 99, 437, 632
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 3 cut(s) 191, 627, 634
TseFI GTSAC 1 cut(s) 36
TseI GCWGC 2 cut(s) 534, 644
Tsp45I GTSAC 1 cut(s) 36
TspRI CASTG 3 cut(s) 191, 627, 634
XapI RAATTY 2 cut(s) 437, 632
XmiI GTMKAC 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 6 cut(s) 5, 27, 297, 339, 485, 675
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.