Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009819

Physical Location & Seq

Genomic Coordinates Forward (+)

22375873 .. 22385466

Gene Structure 9594 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1093000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2886 bp

ATGAGGGCTGTTCCGAGGCACGAGCGCCGATGGGCCTCGGACTCCGTCCCCATGAGATCGACGGTCAGCACCGGATCATCATCTCCGGCAATGACCGATGACTCCAACTCTGGGGATCAGGAGTTCGTCGAGGTAACTCTCGACTTACAAGATGACAACACGATTGTCCTCCGCAGCGTCGAGCCTGCCACCGTCATTCACATCGACGAACTCGCCACTGCTGGAAATGAAACTCCGACGTCGGCGTTGGCTTCGGCTTCTCAGTCGCCATCCACGATACGGCGAAGCTCGTCCAACAATCGGATACGGCAGTTTTCTCAGGAGCTCAAAGCCGAGGCCGTAGCGAAAGCGAAGCAATTTTCTCAAGAGCTCAAAGCCGAGCTGAGGAGGTTTTCGTGGAACCAAAGCCACGGCTCGAGAGTTCTCTCTGCTTCGACTTCTCAAAATGCATCGGTCGGTGGTGGCGGAGGCGGTGCGTTTGACTCGGCGCTGGCCGCTCGCGCTCTCAGGCGGCAGCGAGCGCAGCTTGATCGCACTCGCTCCGGCGCTCAGAAAGCGCTTCGGGGGTTAAGGTTCATCAGTAACTGCAAGAGTTCGAAGACCAATGGCGTCGATGCTTGGAACGAAGTGGAGGCCAATTTCAACAAGCTCGCCAAGGACGGTCACCTCCATCGCGCCCATTTCGGTCAATGCATCGGAATGAGAGATTCCAAGGAATTTGCGTTGGAGTTGTTCGATGCGTTGGGTCGTAGACGACGAATGAAGGTTGAGAAAATCAGCAAGGACGAGCTCTATGAGTTTTGGTCCCAAATTTCCGATCAAAGTTTCGATTCCAGACTCCAGATCTTCTTCGACATGGTGGACAAGAACGATGATGGTCGAATTACAGAAGAAGAAGTTAAAGAGATCATCATGTTGAGTGCTTCTGCAAACAAGCTATCAAGATTGAAGGAACAAGCAGAGGAATATGCAGCTCTGATCATGGAAGAGTTGGATCCTGAAAGACTTGGCTACATCGAGTTATGGCAATTGGAGACGCTTCTACTACAAAAAGACACGTACTTAAACTACAGCCAAGCACTCAGTTACACGAGCCAAGCATTGAGCCAGAACCTCCAAGGGCTGAGAAGTAGGAGTCCAATACGCAGAATGAGCACCAAATTACTCTATTACTTGCAGGAAAACTGGAAGAGAATCTGGGTTTTGACATTGTGGGTTAGCATTATGATTACGCTCTTCACATGGAAATTCATCCAGTACAAGAGGAAAAGTGCTTTTCAGATAATGGGTTATTGCCTTCTCACGGCCAAAGGTGCTGGCGAGACCTTGAAATTCAACATGGCTCTTGTGCTGTTGCCTGTTTGTAGAAACACCATCACTTGGCTCAGGTCCACCAGGCTTGGCTTCTTTGTGCCTTTTGACGACAACATCAACTTCCACAAGACAATTGCTGCAGCCATCGTAGTTGGTGTCATTCTCCATGCTGGGAACCATCTTGCTTGTGATTTTCCGAGGCTTATAAAGGTTCCCGAATCAGACTACGAGAAATATTTGCATCATGACTTTGGAAAGCATAAACCCACCTACCTAGATTTGGTTAAAGGGGCAGAGGGGGTGACTGGAATTATAATGGTGATATGCATGATTATTGCTTTCACACTCGCTACACGGTGGTTCAGGCGGAGCCTCATTAAGCTTCCCAAGCCCTTCAATAGGCTCACCGGCTTCAATGCTTTCTGGTATTCACATCACTTATTTGTTATTGTCTACATCTTGCTCATTATCCATGGCGTTTTCCTCTATCTCGTGCACATATGGTACCTAAAGACGACTTGGATGTATCTTTGTGTTCCTGTTTTACTATATGCTGGAGAAAGGATCTTGAGAATCTTTCGGTCCGGCTCATATATTGTCCGTCTTCTGAAGGTTGCTATTTATCCTGGAAACGTTCTCACATTGCAAATGTCTAAGCCACAACAGTTTAAGTACAAGAGTGGTCAGTACATGTTTGTACAGTGCCCAGCAGTTTCTCCGTTTGAGTGGCATCCATTTTCGATTACATCTGCTCCAGGTGATGACTATCTTAGCGTTCACATTCGTCAGCTTGGTGACTGGACACAAGAGCTTAAGAGAGTCTTCTCAGAAGCTTGCGAGCCTCCCTTAGCTGGGAAGAGTGGCCTTCTAAGGGCTGATGAGACAACCAAGACAAGTTTGCCCAAGCTTTTAATAGATGGGCCTTACGGTGCCCCAGCACAAGACTACCGGAAATATGATGTCCTTCTGCTTGTTGGTCTTGGAATCGGTGCAACGCCATTCATCAGTATTTTGAAAGATTTGCTCAACAATATTGTCAAGATGGAAGAGCAGGCAGATTCCGTTTCAGATTTCAGTCGAACATCAGACCTAAGTTCTGGAAGTATAGGTTCTCCCAATCACAACAAGGTTTATCCGAAACGAAAAAAGCCTCTGAAGACCACCAATGCCTACTTTTATTGGGTAACGAGGGAGCAAGGGTCATTTGATTGGTTCAAGGGAGTGATGAATGAAGTTGCAGAGCTTGATCAAAGGGGTGTCATTGAGATGCACAACTACTTGACTAGTGTGTACGAGGAAGGCGATGCTCGATCAACACTTATCACCATGGTTCAAGCTCTCAACCATGCTAAGAATGGGGTGGATATTGTGTCTGGCACAAGGGTGCGGACCCATTTTGCAAGGCCTAATTGGAAGAAAGTTTTCTCAAAAACTTCCTCCAAACACTGTAATGCAAGGATAGGGGTATTCTATTGTGGAGCACCAGTTTTGGCCAAAGAACTCAGCCAGCTTTGCTATGAGTTCAATCAAAAAGGTTCAACCAAGTTCGAATTCCACAAGGAACACTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0001101 GO:0002252 GO:0002376 GO:0002679 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0010941 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0016999 GO:0017000 GO:0017144 GO:0023052 GO:0032870 GO:0033036 GO:0033037 GO:0033500 GO:0033554 GO:0033993 GO:0035556 GO:0042221 GO:0042592 GO:0042743 GO:0043067 GO:0043069 GO:0044237 GO:0044249 GO:0044464 GO:0045730 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050665 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051716 GO:0052542 GO:0052545 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071396 GO:0071470 GO:0071495 GO:0071944 GO:0072593 GO:0097305 GO:0097306 GO:0104004 GO:1901700 GO:1901701 GO:1903409

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

962

Amino Acids

109.27

Weight (kDa)

9.33

Isoelectric Point (pI)

50.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	175 - 278	6.4e-40	Respiratory burst NADPH oxidase
Ferric_reduct	PF01794	446 - 594	1e-11	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	638 - 751	1.4e-36	FAD-binding domain
NAD_binding_6	PF08030	757 - 943	5.9e-52	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0009819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64060
fragaria_vesca	FvH4_5g03310 FvH4_5g03310 FvH4_5g03310 FvH4_5g03310
malus_domestica	MD06G1093000.v1.1 MD14G1113700.v1.1
prunus_persica	Prupe.5G107400_v2.0.a1
pyrus_communis	pycom06g08880
rosa_chinensis	RchiOBHm_Chr7g0196471
rosa_laevigata	RLG00000004012
rosa_multiflora	Rmu_co8333625.1_g000001 Rmu_sc0000736.1_g000003
rosa_roxburghii	Rroxscaffold_3G00259290
rosa_rugosa	Rorug07G0033600 Rorug07G0033700
rosa_samantha	Rh7AG158000 Rh7BG159900 Rh7CG166400 Rh7DG159200
rosa_wichuraiana	Rw7G013810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1520, 1628
AatII	GACGTC	1 cut(s)	242
Acc65I	GGTACC	1 cut(s)	1818
AccB1I	GGYRCC	2 cut(s)	1818, 2243
AccB7I	CCANNNNNTGG	1 cut(s)	1380
AccBSI	CCGCTC	1 cut(s)	497
AccI	GTMKAC	2 cut(s)	751, 1767
AccII	CGCG	2 cut(s)	501, 675
AciI	CCGC	7 cut(s)	172, 465, 471, 495, 511, 1681, 2701
AclI	AACGTT	1 cut(s)	1947
AclWI	GGATC	5 cut(s)	82, 123, 989, 1002, 1886
AcoI	YGGCCR	3 cut(s)	492, 1305, 2805
AcsI	RAATTY	5 cut(s)	716, 810, 1247, 1331, 2864
AcuI	CTGAAG	2 cut(s)	1943, 2489
AcyI	GRCGYC	2 cut(s)	239, 609
AfaI	GTAC	7 cut(s)	1061, 1259, 1820, 1988, 2003, 2013, 2606
AfeI	AGCGCT	1 cut(s)	558
AflII	CTTAAG	1 cut(s)	2126
AflIII	ACRYGT	2 cut(s)	1056, 2004
AhlI	ACTAGT	1 cut(s)	2597
AjnI	CCWGG	3 cut(s)	1394, 1939, 2068
AjuI	GAANNNNNNNTTGG	2 cut(s)	2849, 2881
AleI	CACNNNNGTG	1 cut(s)	2696
AloI	GAACNNNNNNTCC	2 cut(s)	107, 139
Alw21I	GWGCWC	6 cut(s)	327, 372, 792, 1157, 1812, 2797
Alw26I	GTCTC	3 cut(s)	1028, 1316, 2189
Alw44I	GTGCAC	1 cut(s)	1808
AlwI	GGATC	5 cut(s)	82, 123, 989, 1002, 1886
AlwNI	CAGNNNCTG	1 cut(s)	585
Ama87I	CYCGRG	1 cut(s)	415
Aor51HI	AGCGCT	1 cut(s)	558
AoxI	GGCC	9 cut(s)	33, 336, 492, 633, 1305, 2176, 2234, 2717, 2805
ApaLI	GTGCAC	1 cut(s)	1808
ApeKI	GCWGC	6 cut(s)	174, 514, 523, 971, 1451, 1454
ApoI	RAATTY	5 cut(s)	716, 810, 1247, 1331, 2864
Asp700I	GAANNNNTTC	1 cut(s)	2735
Asp718I	GGTACC	1 cut(s)	1818
AspLEI	GCGC	7 cut(s)	27, 490, 503, 523, 548, 559, 677
AspS9I	GGNCC	6 cut(s)	33, 804, 1389, 1896, 2234, 2703
AsuHPI	GGTGA	7 cut(s)	656, 1627, 1645, 1711, 2084, 2120, 2629
AsuII	TTCGAA	2 cut(s)	596, 2862
AvaI	CYCGRG	1 cut(s)	415
AvaII	GGWCC	4 cut(s)	804, 1389, 1896, 2703
BaeGI	GKGCMC	3 cut(s)	1812, 2021, 2248
BaeI	ACNNNNGTAYC	2 cut(s)	296, 329
BalI	TGGCCA	1 cut(s)	2807
BamHI	GGATCC	1 cut(s)	994
BanI	GGYRCC	2 cut(s)	1818, 2243
BanII	GRGCYC	3 cut(s)	327, 372, 792
BauI	CACGAG	3 cut(s)	20, 1090, 1805
BbsI	GAAGAC	4 cut(s)	605, 1910, 2128, 2477
Bbv12I	GWGCWC	6 cut(s)	327, 372, 792, 1157, 1812, 2797
BbvCI	CCTCAGC	1 cut(s)	383
BbvI	GCAGC	6 cut(s)	186, 526, 535, 983, 1438, 1466
BccI	CCATC	9 cut(s)	24, 277, 678, 869, 1382, 1466, 1500, 2225, 2350
BceAI	ACGGC	5 cut(s)	296, 323, 323, 427, 1320
BcgI	CGANNNNNNTGC	2 cut(s)	512, 546
BciT130I	CCWGG	3 cut(s)	1396, 1941, 2070
BciVI	GTATCC	1 cut(s)	297
BclI	TGATCA	2 cut(s)	978, 2560
BcoDI	GTCTC	3 cut(s)	1028, 1316, 2189
BcuI	ACTAGT	1 cut(s)	2597
BfaI	CTAG	2 cut(s)	1589, 2598
BfmI	CTRYAG	2 cut(s)	1069, 1452
BfoI	RGCGCY	4 cut(s)	28, 491, 549, 560
BfrI	CTTAAG	1 cut(s)	2126
BfuI	GTATCC	1 cut(s)	297
BglII	AGATCT	1 cut(s)	843
BisI	GCNGC	8 cut(s)	175, 495, 512, 515, 524, 972, 1452, 1455
BlsI	GCNGC	8 cut(s)	176, 496, 513, 516, 525, 973, 1453, 1456
Bme1390I	CCNGG	3 cut(s)	1396, 1941, 2070
Bme18I	GGWCC	4 cut(s)	804, 1389, 1896, 2703
BmeT110I	CYCGRG	1 cut(s)	415
BmgT120I	GGNCC	6 cut(s)	33, 804, 1389, 1896, 2234, 2703
BmiI	GGNNCC	9 cut(s)	401, 806, 996, 1490, 1527, 1685, 1820, 2245, 2705
BmrFI	CCNGG	3 cut(s)	1396, 1941, 2070
BmsI	GCATC	8 cut(s)	458, 604, 702, 727, 1564, 2053, 2571, 2608
BpiI	GAAGAC	4 cut(s)	605, 1910, 2128, 2477
BpmI	CTGGAG	3 cut(s)	824, 1890, 2052
Bpu10I	CCTNAGC	3 cut(s)	383, 1385, 2161
Bpu14I	TTCGAA	2 cut(s)	596, 2862
BpuEI	CTTGAG	2 cut(s)	348, 1903
BsaAI	YACGTR	1 cut(s)	1059
BsaBI	GATNNNNATC	2 cut(s)	79, 2079
BsaHI	GRCGYC	2 cut(s)	239, 609
BsaI	GGTCTC	1 cut(s)	1316
BsaWI	WCCGGW	2 cut(s)	71, 2262
BsaXI	ACNNNNNCTCC	6 cut(s)	2050, 2080, 2498, 2528, 2784, 2814
Bse118I	RCCGGY	1 cut(s)	1721
Bse1I	ACTGG	5 cut(s)	1190, 1255, 1624, 2117, 2798
Bse3DI	GCAATG	2 cut(s)	96, 1955
Bse8I	GATNNNNATC	2 cut(s)	79, 2079
BseBI	CCWGG	3 cut(s)	1396, 1941, 2070
BseGI	GGATG	4 cut(s)	269, 1251, 1842, 2044
BseJI	GATNNNNATC	2 cut(s)	79, 2079
BseMI	GCAATG	2 cut(s)	96, 1955
BseNI	ACTGG	5 cut(s)	1190, 1255, 1624, 2117, 2798
BseRI	GAGGAG	1 cut(s)	400
BseSI	GKGCMC	3 cut(s)	1812, 2021, 2248
BseXI	GCAGC	6 cut(s)	186, 526, 535, 983, 1438, 1466
BseYI	CCCAGC	4 cut(s)	1484, 2020, 2165, 2248
Bsh1236I	CGCG	2 cut(s)	501, 675
Bsh1285I	CGRYCG	1 cut(s)	456
BshFI	GGCC	9 cut(s)	35, 338, 494, 635, 1307, 2178, 2236, 2719, 2807
BshNI	GGYRCC	2 cut(s)	1818, 2243
BsiEI	CGRYCG	1 cut(s)	456
BsiHKAI	GWGCWC	6 cut(s)	327, 372, 792, 1157, 1812, 2797
BsiHKCI	CYCGRG	1 cut(s)	415
BsiSI	CCGG	6 cut(s)	72, 86, 543, 1722, 1899, 2263
BslFI	GGGAC	2 cut(s)	32, 790
BsmAI	GTCTC	3 cut(s)	1028, 1316, 2189
BsmBI	CGTCTC	1 cut(s)	1028
BsmFI	GGGAC	2 cut(s)	32, 790
BsnI	GGCC	9 cut(s)	35, 338, 494, 635, 1307, 2178, 2236, 2719, 2807
Bso31I	GGTCTC	1 cut(s)	1316
BsoBI	CYCGRG	1 cut(s)	415
Bsp119I	TTCGAA	2 cut(s)	596, 2862
Bsp1286I	GDGCHC	8 cut(s)	327, 372, 792, 1157, 1812, 2021, 2248, 2797
Bsp1407I	TGTACA	1 cut(s)	2011
Bsp19I	CCATGG	2 cut(s)	1786, 2640
BspACI	CCGC	7 cut(s)	172, 465, 471, 495, 511, 1681, 2701
BspANI	GGCC	9 cut(s)	35, 338, 494, 635, 1307, 2178, 2236, 2719, 2807
BspFNI	CGCG	2 cut(s)	501, 675
BspHI	TCATGA	1 cut(s)	1558
BspLI	GGNNCC	9 cut(s)	401, 806, 996, 1490, 1527, 1685, 1820, 2245, 2705
BspMAI	CTGCAG	1 cut(s)	1456
BspPI	GGATC	5 cut(s)	82, 123, 989, 1002, 1886
BspQI	GCTCTTC	2 cut(s)	1241, 2355
BspT104I	TTCGAA	2 cut(s)	596, 2862
BspT107I	GGYRCC	2 cut(s)	1818, 2243
BspTI	CTTAAG	1 cut(s)	2126
BspTNI	GGTCTC	1 cut(s)	1316
BsrBI	CCGCTC	1 cut(s)	497
BsrDI	GCAATG	2 cut(s)	96, 1955
BsrFI	RCCGGY	1 cut(s)	1721
BsrGI	TGTACA	1 cut(s)	2011
BsrI	ACTGG	5 cut(s)	1190, 1255, 1624, 2117, 2798
BssAI	RCCGGY	1 cut(s)	1721
BssNI	GRCGYC	2 cut(s)	239, 609
BssSI	CACGAG	3 cut(s)	20, 1090, 1805
BssT1I	CCWWGG	5 cut(s)	654, 711, 1117, 1786, 2640
Bst2BI	CACGAG	3 cut(s)	20, 1090, 1805
Bst2UI	CCWGG	3 cut(s)	1396, 1941, 2070
Bst4CI	ACNGT	8 cut(s)	64, 193, 662, 1671, 1980, 2016, 2243, 2762
Bst6I	CTCTTC	5 cut(s)	981, 1184, 1241, 2165, 2355
BstACI	GRCGYC	2 cut(s)	239, 609
BstAFI	CTTAAG	1 cut(s)	2126
BstAUI	TGTACA	1 cut(s)	2011
BstBAI	YACGTR	1 cut(s)	1059
BstBI	TTCGAA	2 cut(s)	596, 2862
BstDSI	CCRYGG	3 cut(s)	409, 1786, 2640
BstEII	GGTNACC	1 cut(s)	662
BstENI	CCTNNNNNAGG	1 cut(s)	1711
BstF5I	GGATG	4 cut(s)	269, 1251, 1842, 2044
BstFNI	CGCG	2 cut(s)	501, 675
BstH2I	RGCGCY	4 cut(s)	28, 491, 549, 560
BstHHI	GCGC	7 cut(s)	27, 490, 503, 523, 548, 559, 677
BstMAI	GTCTC	3 cut(s)	1028, 1316, 2189
BstMCI	CGRYCG	1 cut(s)	456
BstMWI	GCNNNNNNNGC	9 cut(s)	494, 500, 520, 523, 554, 1152, 1313, 1702, 2826
BstNI	CCWGG	3 cut(s)	1396, 1941, 2070
BstNSI	RCATGY	1 cut(s)	2008
BstPI	GGTNACC	1 cut(s)	662
BstSCI	CCNGG	3 cut(s)	1394, 1939, 2068
BstSFI	CTRYAG	2 cut(s)	1069, 1452
BstSLI	GKGCMC	3 cut(s)	1812, 2021, 2248
BstUI	CGCG	2 cut(s)	501, 675
BstV1I	GCAGC	6 cut(s)	186, 526, 535, 983, 1438, 1466
BstV2I	GAAGAC	4 cut(s)	605, 1910, 2128, 2477
BstX2I	RGATCY	3 cut(s)	843, 994, 1878
BstYI	RGATCY	3 cut(s)	843, 994, 1878
BsuI	GTATCC	1 cut(s)	297
BsuRI	GGCC	9 cut(s)	35, 338, 494, 635, 1307, 2178, 2236, 2719, 2807
BtgI	CCRYGG	3 cut(s)	409, 1786, 2640
BtgZI	GCGATG	2 cut(s)	656, 2631
BtsCI	GGATG	4 cut(s)	269, 1251, 1842, 2044
BtsI	GCAGTG	1 cut(s)	216
BtsIMutI	CAGTG	3 cut(s)	216, 2021, 2758
CaiI	CAGNNNCTG	1 cut(s)	585
CciI	TCATGA	1 cut(s)	1558
CfoI	GCGC	7 cut(s)	27, 490, 503, 523, 548, 559, 677
Cfr10I	RCCGGY	1 cut(s)	1721
Cfr13I	GGNCC	6 cut(s)	33, 804, 1389, 1896, 2234, 2703
CpoI	CGGWCCG	1 cut(s)	1896
CseI	GACGC	3 cut(s)	166, 598, 1045
Csp6I	GTAC	7 cut(s)	1060, 1258, 1819, 1987, 2002, 2012, 2605
CspCI	CAANNNNNGTGG	2 cut(s)	1381, 1416
CspI	CGGWCCG	1 cut(s)	1896
CviQI	GTAC	7 cut(s)	1060, 1258, 1819, 1987, 2002, 2012, 2605
EaeI	YGGCCR	3 cut(s)	492, 1305, 2805
Eam1104I	CTCTTC	5 cut(s)	981, 1184, 1241, 2165, 2355
EarI	CTCTTC	5 cut(s)	981, 1184, 1241, 2165, 2355
EciI	GGCGGA	2 cut(s)	480, 1696
Ecl136II	GAGCTC	3 cut(s)	325, 370, 790
Eco130I	CCWWGG	5 cut(s)	654, 711, 1117, 1786, 2640
Eco147I	AGGCCT	1 cut(s)	2719
Eco24I	GRGCYC	3 cut(s)	327, 372, 792
Eco31I	GGTCTC	1 cut(s)	1316
Eco47I	GGWCC	4 cut(s)	804, 1389, 1896, 2703
Eco47III	AGCGCT	1 cut(s)	558
Eco53kI	GAGCTC	3 cut(s)	325, 370, 790
Eco57I	CTGAAG	2 cut(s)	1943, 2489
Eco88I	CYCGRG	1 cut(s)	415
Eco91I	GGTNACC	1 cut(s)	662
EcoICRI	GAGCTC	3 cut(s)	325, 370, 790
EcoNI	CCTNNNNNAGG	1 cut(s)	1711
EcoO65I	GGTNACC	1 cut(s)	662
EcoRI	GAATTC	1 cut(s)	2864
EcoRII	CCWGG	3 cut(s)	1394, 1939, 2068
EcoT14I	CCWWGG	5 cut(s)	654, 711, 1117, 1786, 2640
EcoT22I	ATGCAT	3 cut(s)	451, 695, 1643
EcoT38I	GRGCYC	3 cut(s)	327, 372, 792
ErhI	CCWWGG	5 cut(s)	654, 711, 1117, 1786, 2640
Esp3I	CGTCTC	1 cut(s)	1028
FalI	AAGNNNNNCTT	3 cut(s)	2120, 2152, 2864
FaqI	GGGAC	2 cut(s)	32, 790
FauNDI	CATATG	1 cut(s)	1814
FbaI	TGATCA	2 cut(s)	978, 2560
FblI	GTMKAC	2 cut(s)	751, 1767
Fnu4HI	GCNGC	8 cut(s)	175, 495, 512, 515, 524, 972, 1452, 1455
FokI	GGATG	4 cut(s)	256, 1238, 1849, 2031
FriOI	GRGCYC	3 cut(s)	327, 372, 792
Fsp4HI	GCNGC	8 cut(s)	175, 495, 512, 515, 524, 972, 1452, 1455
FspBI	CTAG	2 cut(s)	1589, 2598
GlaI	GCGC	7 cut(s)	26, 489, 502, 522, 547, 558, 676
GluI	GCNGC	8 cut(s)	175, 495, 512, 515, 524, 972, 1452, 1455
GsaI	CCCAGC	4 cut(s)	1488, 2024, 2169, 2252
GsuI	CTGGAG	3 cut(s)	824, 1890, 2052
HaeII	RGCGCY	4 cut(s)	28, 491, 549, 560
HaeIII	GGCC	9 cut(s)	35, 338, 494, 635, 1307, 2178, 2236, 2719, 2807
HapII	CCGG	6 cut(s)	72, 86, 543, 1722, 1899, 2263
HgaI	GACGC	3 cut(s)	166, 598, 1045
HhaI	GCGC	7 cut(s)	27, 490, 503, 523, 548, 559, 677
Hin1I	GRCGYC	2 cut(s)	239, 609
Hin6I	GCGC	7 cut(s)	25, 488, 501, 521, 546, 557, 675
HinP1I	GCGC	7 cut(s)	25, 488, 501, 521, 546, 557, 675
HindIII	AAGCTT	3 cut(s)	1694, 2145, 2219
HpaII	CCGG	6 cut(s)	72, 86, 543, 1722, 1899, 2263
HphI	GGTGA	7 cut(s)	656, 1627, 1645, 1711, 2084, 2120, 2629
Hpy166II	GTNNAC	7 cut(s)	752, 862, 1392, 1768, 1810, 2092, 2605
Hpy8I	GTNNAC	7 cut(s)	752, 862, 1392, 1768, 1810, 2092, 2605
Hpy99I	CGWCG	8 cut(s)	64, 131, 182, 209, 241, 244, 614, 759
HpyAV	CCTTC	8 cut(s)	757, 943, 1307, 1717, 1918, 2189, 2288, 2606
HpyCH4III	ACNGT	8 cut(s)	64, 193, 662, 1671, 1980, 2016, 2243, 2762
HpyCH4IV	ACGT	3 cut(s)	239, 1058, 1947
HpyF10VI	GCNNNNNNNGC	9 cut(s)	494, 500, 520, 523, 554, 1152, 1313, 1702, 2826
HpySE526I	ACGT	3 cut(s)	239, 1058, 1947
Hsp92I	GRCGYC	2 cut(s)	239, 609
HspAI	GCGC	7 cut(s)	25, 488, 501, 521, 546, 557, 675
KpnI	GGTACC	1 cut(s)	1822
Ksp22I	TGATCA	2 cut(s)	978, 2560
LguI	GCTCTTC	2 cut(s)	1241, 2355
LmnI	GCTCC	6 cut(s)	322, 545, 1683, 2071, 2506, 2792
Lsp1109I	GCAGC	6 cut(s)	186, 526, 535, 983, 1438, 1466
LweI	GCATC	8 cut(s)	458, 604, 702, 727, 1564, 2053, 2571, 2608
MaeI	CTAG	2 cut(s)	1589, 2598
MaeII	ACGT	3 cut(s)	239, 1058, 1947
MaeIII	GTNAC	7 cut(s)	133, 581, 662, 1085, 1615, 2108, 2497
MbiI	CCGCTC	1 cut(s)	497
MfeI	CAATTG	2 cut(s)	1028, 1446
MflI	RGATCY	3 cut(s)	843, 994, 1878
MhlI	GDGCHC	8 cut(s)	327, 372, 792, 1157, 1812, 2021, 2248, 2797
MlsI	TGGCCA	1 cut(s)	2807
MluNI	TGGCCA	1 cut(s)	2807
MlyI	GAGTC	6 cut(s)	35, 95, 476, 831, 1144, 2142
MmeI	TCCRAC	5 cut(s)	129, 260, 318, 705, 972
Mox20I	TGGCCA	1 cut(s)	2807
Mph1103I	ATGCAT	3 cut(s)	451, 695, 1643
MroXI	GAANNNNTTC	1 cut(s)	2735
MscI	TGGCCA	1 cut(s)	2807
MseI	TTAA	8 cut(s)	569, 900, 1064, 1599, 1692, 1983, 2127, 2225
MslI	CAYNNNNRTG	4 cut(s)	698, 2579, 2696, 2763
Msp20I	TGGCCA	1 cut(s)	2807
MspCI	CTTAAG	1 cut(s)	2126
MspI	CCGG	6 cut(s)	72, 86, 543, 1722, 1899, 2263
MspR9I	CCNGG	3 cut(s)	1396, 1941, 2070
MunI	CAATTG	2 cut(s)	1028, 1446
MvaI	CCWGG	3 cut(s)	1396, 1941, 2070
MvnI	CGCG	2 cut(s)	501, 675
MwoI	GCNNNNNNNGC	9 cut(s)	494, 500, 520, 523, 554, 1152, 1313, 1702, 2826
NcoI	CCATGG	2 cut(s)	1786, 2640
NdeI	CATATG	1 cut(s)	1814
NlaIV	GGNNCC	9 cut(s)	401, 806, 996, 1490, 1527, 1685, 1820, 2245, 2705
NmeAIII	GCCGAG	3 cut(s)	358, 403, 464
NmuCI	GTSAC	3 cut(s)	662, 1615, 2108
NsiI	ATGCAT	3 cut(s)	451, 695, 1643
NspI	RCATGY	1 cut(s)	2008
NspV	TTCGAA	2 cut(s)	596, 2862
OliI	CACNNNNGTG	1 cut(s)	2696
PaeR7I	CTCGAG	1 cut(s)	415
PagI	TCATGA	1 cut(s)	1558
PceI	AGGCCT	1 cut(s)	2719
PciI	ACATGT	1 cut(s)	2004
PciSI	GCTCTTC	2 cut(s)	1241, 2355
PcsI	WCGNNNNNNNCGW	3 cut(s)	272, 754, 2613
PdmI	GAANNNNTTC	1 cut(s)	2735
PfeI	GAWTC	7 cut(s)	707, 830, 1194, 1532, 1887, 2298, 2372
PflFI	GACNNNGTC	1 cut(s)	44
PflMI	CCANNNNNTGG	1 cut(s)	1380
PfoI	TCCNGGA	1 cut(s)	1939
PkrI	GCNGC	8 cut(s)	176, 496, 513, 516, 525, 973, 1453, 1456
PleI	GAGTC	6 cut(s)	35, 95, 476, 831, 1143, 2141
PpsI	GAGTC	6 cut(s)	35, 95, 476, 831, 1143, 2141
Ppu21I	YACGTR	1 cut(s)	1059
PscI	ACATGT	1 cut(s)	2004
PsiI	TTATAA	2 cut(s)	1520, 1628
Psp124BI	GAGCTC	3 cut(s)	327, 372, 792
Psp1406I	AACGTT	1 cut(s)	1947
Psp6I	CCWGG	3 cut(s)	1394, 1939, 2068
PspEI	GGTNACC	1 cut(s)	662
PspFI	CCCAGC	4 cut(s)	1484, 2020, 2165, 2248
PspGI	CCWGG	3 cut(s)	1394, 1939, 2068
PspN4I	GGNNCC	9 cut(s)	401, 806, 996, 1490, 1527, 1685, 1820, 2245, 2705
PspPI	GGNCC	6 cut(s)	33, 804, 1389, 1896, 2234, 2703
PstI	CTGCAG	1 cut(s)	1456
PstNI	CAGNNNCTG	1 cut(s)	585
PsuI	RGATCY	3 cut(s)	843, 994, 1878
PsyI	GACNNNGTC	1 cut(s)	44
RsaI	GTAC	7 cut(s)	1061, 1259, 1820, 1988, 2003, 2013, 2606
RsaNI	GTAC	7 cut(s)	1060, 1258, 1819, 1987, 2002, 2012, 2605
RseI	CAYNNNNRTG	4 cut(s)	698, 2579, 2696, 2763
Rsr2I	CGGWCCG	1 cut(s)	1896
RsrII	CGGWCCG	1 cut(s)	1896
SacI	GAGCTC	3 cut(s)	327, 372, 792
SapI	GCTCTTC	2 cut(s)	1241, 2355
SaqAI	TTAA	8 cut(s)	569, 900, 1064, 1599, 1692, 1983, 2127, 2225
SatI	GCNGC	8 cut(s)	175, 495, 512, 515, 524, 972, 1452, 1455
Sau96I	GGNCC	6 cut(s)	33, 804, 1389, 1896, 2234, 2703
SchI	GAGTC	6 cut(s)	35, 95, 476, 831, 1144, 2142
ScrFI	CCNGG	3 cut(s)	1396, 1941, 2070
SduI	GDGCHC	8 cut(s)	327, 372, 792, 1157, 1812, 2021, 2248, 2797
SfaNI	GCATC	8 cut(s)	458, 604, 702, 727, 1564, 2053, 2571, 2608
SfcI	CTRYAG	2 cut(s)	1069, 1452
Sfr274I	CTCGAG	1 cut(s)	415
SfuI	TTCGAA	2 cut(s)	596, 2862
SinI	GGWCC	4 cut(s)	804, 1389, 1896, 2703
SlaI	CTCGAG	1 cut(s)	415
SmiMI	CAYNNNNRTG	4 cut(s)	698, 2579, 2696, 2763
SmlI	CTYRAG	4 cut(s)	363, 415, 1882, 2126
SmoI	CTYRAG	4 cut(s)	363, 415, 1882, 2126
SpeI	ACTAGT	1 cut(s)	2597
SseBI	AGGCCT	1 cut(s)	2719
SsiI	CCGC	7 cut(s)	172, 465, 471, 495, 511, 1681, 2701
SspI	AATATT	2 cut(s)	1550, 2347
SspMI	CTAG	2 cut(s)	1589, 2598
SstI	GAGCTC	3 cut(s)	327, 372, 792
StuI	AGGCCT	1 cut(s)	2719
StyD4I	CCNGG	3 cut(s)	1394, 1939, 2068
StyI	CCWWGG	5 cut(s)	654, 711, 1117, 1786, 2640
TaaI	ACNGT	8 cut(s)	64, 193, 662, 1671, 1980, 2016, 2243, 2762
TaiI	ACGT	3 cut(s)	242, 1061, 1950
TaqII	GACCGA	4 cut(s)	110, 442, 674, 1884
TatI	WGTACW	4 cut(s)	1257, 1986, 2001, 2011
TauI	GCSGC	2 cut(s)	497, 514
TfiI	GAWTC	7 cut(s)	707, 830, 1194, 1532, 1887, 2298, 2372
Tru1I	TTAA	8 cut(s)	569, 900, 1064, 1599, 1692, 1983, 2127, 2225
Tru9I	TTAA	8 cut(s)	569, 900, 1064, 1599, 1692, 1983, 2127, 2225
TscAI	CASTG	3 cut(s)	223, 2021, 2765
TseFI	GTSAC	3 cut(s)	662, 1615, 2108
TseI	GCWGC	6 cut(s)	174, 514, 523, 971, 1451, 1454
Tsp45I	GTSAC	3 cut(s)	662, 1615, 2108
TspDTI	ATGAA	7 cut(s)	243, 565, 776, 1240, 2305, 2555, 2559
TspGWI	ACGGA	4 cut(s)	34, 1904, 2022, 2365
TspRI	CASTG	3 cut(s)	223, 2021, 2765
Tth111I	GACNNNGTC	1 cut(s)	44
Van91I	CCANNNNNTGG	1 cut(s)	1380
Vha464I	CTTAAG	1 cut(s)	2126
VneI	GTGCAC	1 cut(s)	1808
VpaK11BI	GGWCC	4 cut(s)	804, 1389, 1896, 2703
XagI	CCTNNNNNAGG	1 cut(s)	1711
XapI	RAATTY	5 cut(s)	716, 810, 1247, 1331, 2864
XceI	RCATGY	1 cut(s)	2008
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2666
XhoI	CTCGAG	1 cut(s)	415
XmiI	GTMKAC	2 cut(s)	751, 1767
XmnI	GAANNNNTTC	1 cut(s)	2735
XspI	CTAG	2 cut(s)	1589, 2598
ZraI	GACGTC	1 cut(s)	240
Zsp2I	ATGCAT	3 cut(s)	451, 695, 1643

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.