Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0009819

Physical Location & Seq

Genomic Coordinates Forward (+)

18347131 .. 18359289

Gene Structure 12159 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1113700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2880 bp

ATGAGGGCTCATCCGAGGCACGAGCGCCGATGGGCTTCGGACTCCGTCCCAGAGAGGTCCATGGTCAGCGCCGGATCATCATCTCCGGCAATGACCGACGACTCCAACTCCAGGGATCAGGAGTTCGTCGAGGTAACTCTCGACTTACAAGATGACAACACGATTGTCCTCCGCAGCGTCGAGCCCACCACCGTCATTCACATCGATGACCTCGCAGGCGCCGGAAATGAAACTCCGACGTCGGCTTCAGCTTCTTGGTCGCCATCCCCGATACGGAGAAGCTCGTCCAACAATCGGATACGGCAATTCTCTCAGGAGCTCAAAGCAGAGGCCGTGGCCAAAGCGAAGCAATTTTCTCAAGAGCTCAAAGCCGAGCTGAGGAGGTTTTCGTGGAGCCAAAGCCACAGCTCGAGAGTTCTATCCGCTTCAACTTCTCAGAATGCATCAGCCGGTGGCGGCGGAGGCGGTGCGTTTGACTTGGCGCTGGCCGCTCGCGCTCTGAGGCGGCAGCGAGCACAGCTCGATCGCACTCGCTCCGGCGCTGAGAAAGCGCTTCGGGGATTAAGGTTCATTGGTAACTACAAGAGTACGAAGACCAATGGCGTCGATGCTTGGAACGAAGTGGAGACCAATTTCAACAAGCTCGCCAAAGACGGCCATCTCTTCCGCGCCGATTTCCCTCAATGCATCGGAATGAGAGATTCGAAAGAGTTTGCGTTGGAGTTGTTCGATGCGTTGGGTCGTAGACGCAGAATGCAGGTTGAGAAAATCAGCAAGGACGAGCTCTATGAGTTTTGGTCCCAAATTTCCGATCAAAGTTTCGATTCGAGGCTCCAGATCTTCTTCGACATGGTGGACAAGAATGACGACGGTCGAATTACAGAAGAAGAAGTAAAAGAGATCATCATGTTGAGTGCTTCTGCAAACAAGCTATCAAGATTGAAGGAACAAGCAGAGGAATATGCAGCTTTGATCATGGAAGAGTTAGATCCTGAAAGCCTTGGCTACATTGAGTTATGGCAATTGGAGACGCTTCTACTACAAAAAGATACGTACTTCAACTACAGCCAAGCACTAAGCTACACGAGCCAAGCCCTAAGCCAGAACCTCCAAGGGCTGAGAAGGAGGAGTCCAATACGCAAAATGAGCACCAACTTAATCTATTATTTGCAGGAAAATTGGAGGAGAGTCTGGGTTTTGACATTGTGGTTTTGCATTATGATTGCGCTCTTCATATGGAAATTCATACAGTACAAGCAAAAAAGTGCTTTTCAGATAATGGGTTATTGCCTTCTCACGGCCAAAGGTGCTGGCGAGACCTTGAAATTCAACATGGCTCTTGTGCTGTTGCCTGTTTGTAGAAACACCATCACTCGGCTCAGGTCCACCAGGCTTGGCTTCTTTGTACCTTTTGACGACAACATCAACTTCCACAAGACAATTGCTGCGGCCATTGTAGTTGGTGTCATTCTCCATGCTGGGAACCATCTCGCTTGTGATTTTCCGAGGCTTATAAAGGTTTCCGAATCAGTCTACAAGAAGCATTTGCTTCACGACTTTGGAAAGAATAAACCCAGCTATCTAGATTTGATTAAAGGGCCAGAGGGGCTGACTGGAATTATAATGTTGATATGCATGATTATTGCTTTCACGCTCGCTACACGGTGGTTCAGGCGTAACCTCGTTAAGCTTCCCAAGCCCTTCAATAGGCTCACTGGCTTCAATGCCTTCTGGTATTCACATCACTTGTTTGTTATTGTCTATGTCTTGCTCATTATCCATGGCGTTTTCCTCTATCTCGTGCACACGTGGTACCTAAAGACGACTTGGATGTATCTTTCTGTTCCTGTTTTACTATATGCTGGAGAAAGAATCTTGAGAATCTTCCGGTCTGGCTTCTATACAGTCCGTCTTCTGAAGGTTGCTATTTATCCTGGAAACGTTCTCACATTGCAAATGTCGAAGCCACAACAGTTTAAGTACAAGAGTGGACAGTACATGTTTGTACAGTGCCCAGCAGTTTCTCCGTTTGAGTGGCATCCATTTTCAATTACCTCAGCTCCAGGCGATGACTACCTTAGTGTTCACATTCGTCAGCTTGGTGACTGGACGCAAGAGCTTAAGAGGGTTTTCTCAGAAGCTTGTGAGCCTTCCTTAGCTGGGAAGAGTGGTCTTCTGAGGGCTGATGAGACAACCAAGACAAGTTTGCCCAAGCTTTTAATAGATGGGCCTTATGGTGCCCCTGCCCAAGACTACCGGAAATATGATGTCCTGCTGCTTGTTGGTCTTGGAATCGGTGCAACGCCATTCATCAGTATTTTAAAAGATTTGCTCAACAATATTGTCAAGATGGAAGAGCAGGCAGATTCTGTTTCAGATTTCAATAGAACATCAGACCTAAGTTTTGGGAGTATAGATTCTCCCAATCCCAACAAGGTTTATCCAAAACGAAAAAAGCCTCTGAAGACCACTAATGCCTACTTTTATTGGGTAACAAGGGAGCAAGGCTCATTTGATTGGTTCAAAGGAGTGATGAATGAAGTTGCAGAGCTTGATCAAAGGGGTGTCATTGAGATGCACAACTACTTGACTAGTGTGTACGAGGAAGGTGATGCTCGATCAACACTCATCACCATGGTTCAAGCTCTCAACCATGCAAAGAATGGGGTGGACATTGTGTCTGGCACAAGGGTGCGGACCCATTTTGCAAGGCCTAATTGGAAGAAAGTTTTCTCAAAAACTTCCTCCAAACACTGTAATGCAAGGATAGGGGTATTCTATTGTGGAGCACCAGTTTTGGCAAAAGAACTCAGCCAGCTCTGCTATGACTTCAATCAAACAGGTTCAACCAAGTTCGAATTCCACAAGGAACACTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0001101 GO:0002252 GO:0002376 GO:0002679 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0010941 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0016999 GO:0017000 GO:0017144 GO:0023052 GO:0032870 GO:0033036 GO:0033037 GO:0033500 GO:0033554 GO:0033993 GO:0035556 GO:0042221 GO:0042592 GO:0042743 GO:0043067 GO:0043069 GO:0044237 GO:0044249 GO:0044464 GO:0045730 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050665 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051716 GO:0052542 GO:0052545 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071396 GO:0071470 GO:0071495 GO:0071944 GO:0072593 GO:0097305 GO:0097306 GO:0104004 GO:1901700 GO:1901701 GO:1903409

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

960

Amino Acids

109.37

Weight (kDa)

9.28

Isoelectric Point (pI)

50.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	173 - 276	1.3e-38	Respiratory burst NADPH oxidase
Ferric_reduct	PF01794	445 - 592	4.4e-13	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	634 - 749	8.6e-37	FAD-binding domain
NAD_binding_6	PF08030	755 - 941	5.9e-52	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64060
fragaria_vesca	FvH4_5g03310 FvH4_5g03310 FvH4_5g03310 FvH4_5g03310
malus_domestica	MD06G1093000.v1.1 MD14G1113700.v1.1
prunus_persica	Prupe.5G107400_v2.0.a1
pyrus_communis	pycom06g08880
rosa_chinensis	RchiOBHm_Chr7g0196471
rosa_laevigata	RLG00000004012
rosa_multiflora	Rmu_co8333625.1_g000001 Rmu_sc0000736.1_g000003
rosa_roxburghii	Rroxscaffold_3G00259290
rosa_rugosa	Rorug07G0033600 Rorug07G0033700
rosa_samantha	Rh7AG158000 Rh7BG159900 Rh7CG166400 Rh7DG159200
rosa_wichuraiana	Rw7G013810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1514, 1622
AatII	GACGTC	1 cut(s)	242
Acc36I	ACCTGC	1 cut(s)	748
Acc65I	GGTACC	1 cut(s)	1812
AccB1I	GGYRCC	3 cut(s)	218, 1812, 2237
AccBSI	CCGCTC	1 cut(s)	491
AccI	GTMKAC	2 cut(s)	745, 1533
AccII	CGCG	2 cut(s)	495, 669
AclI	AACGTT	1 cut(s)	1941
AclWI	GGATC	3 cut(s)	82, 123, 983
AcoI	YGGCCR	5 cut(s)	336, 486, 655, 1299, 1449
AcsI	RAATTY	4 cut(s)	804, 1241, 1325, 2858
AcuI	CTGAAG	3 cut(s)	231, 1937, 2483
AcvI	CACGTG	1 cut(s)	1809
AcyI	GRCGYC	3 cut(s)	219, 239, 603
AfaI	GTAC	9 cut(s)	589, 1055, 1253, 1407, 1814, 1982, 1997, 2007, 2600
AfeI	AGCGCT	1 cut(s)	552
AfiI	CCNNNNNNNGG	8 cut(s)	111, 273, 294, 378, 1297, 1374, 1707, 2160
AflII	CTTAAG	1 cut(s)	2120
AflIII	ACRYGT	2 cut(s)	1806, 1998
AhdI	GACNNNNNGTC	1 cut(s)	2677
AhlI	ACTAGT	1 cut(s)	2591
AjnI	CCWGG	4 cut(s)	110, 1388, 1933, 2062
AjuI	GAANNNNNNNTTGG	2 cut(s)	2843, 2875
AleI	CACNNNNGTG	1 cut(s)	2690
AloI	GAACNNNNNNTCC	2 cut(s)	107, 139
Alw21I	GWGCWC	7 cut(s)	321, 366, 517, 786, 1151, 1806, 2791
Alw26I	GTCTC	4 cut(s)	620, 1022, 1310, 2183
Alw44I	GTGCAC	1 cut(s)	1802
AlwI	GGATC	3 cut(s)	82, 123, 983
AlwNI	CAGNNNCTG	1 cut(s)	2369
Ama87I	CYCGRG	1 cut(s)	409
Aor51HI	AGCGCT	1 cut(s)	552
AoxI	GGCC	9 cut(s)	330, 336, 486, 655, 1299, 1449, 1598, 2228, 2711
ApaLI	GTGCAC	1 cut(s)	1802
ApeKI	GCWGC	5 cut(s)	174, 508, 965, 1445, 2275
ApoI	RAATTY	4 cut(s)	804, 1241, 1325, 2858
ArsI	GACNNNNNNTTYG	2 cut(s)	2387, 2419
Asp700I	GAANNNNTTC	1 cut(s)	2729
Asp718I	GGTACC	1 cut(s)	1812
AspLEI	GCGC	9 cut(s)	27, 71, 221, 484, 497, 542, 553, 671, 1228
AspS9I	GGNCC	6 cut(s)	57, 798, 1383, 1598, 2228, 2697
AsuHPI	GGTGA	3 cut(s)	2114, 2621, 2623
AsuII	TTCGAA	2 cut(s)	704, 2856
AvaI	CYCGRG	1 cut(s)	409
AvaII	GGWCC	4 cut(s)	57, 798, 1383, 2697
BaeGI	GKGCMC	3 cut(s)	1806, 2015, 2242
BalI	TGGCCA	1 cut(s)	338
BanI	GGYRCC	3 cut(s)	218, 1812, 2237
BanII	GRGCYC	5 cut(s)	10, 186, 321, 366, 786
BauI	CACGAG	3 cut(s)	20, 1084, 1799
BbrPI	CACGTG	1 cut(s)	1809
BbsI	GAAGAC	4 cut(s)	599, 1904, 2165, 2471
Bbv12I	GWGCWC	7 cut(s)	321, 366, 517, 786, 1151, 1806, 2791
BbvCI	CCTCAGC	2 cut(s)	377, 2056
BbvI	GCAGC	5 cut(s)	186, 520, 977, 1432, 2262
BccI	CCATC	7 cut(s)	24, 271, 666, 1376, 1494, 2219, 2344
BceAI	ACGGC	4 cut(s)	317, 317, 670, 1314
BciT130I	CCWGG	4 cut(s)	112, 1390, 1935, 2064
BciVI	GTATCC	1 cut(s)	291
BclI	TGATCA	2 cut(s)	972, 2554
BcoDI	GTCTC	4 cut(s)	620, 1022, 1310, 2183
BcuI	ACTAGT	1 cut(s)	2591
BfaI	CTAG	2 cut(s)	1583, 2592
BfmI	CTRYAG	1 cut(s)	1063
BfoI	RGCGCY	6 cut(s)	28, 72, 222, 485, 543, 554
BfrI	CTTAAG	1 cut(s)	2120
BfuAI	ACCTGC	1 cut(s)	748
BfuI	GTATCC	1 cut(s)	291
BglI	GCCNNNNNGGC	1 cut(s)	1606
BglII	AGATCT	1 cut(s)	837
BisI	GCNGC	9 cut(s)	175, 457, 489, 506, 509, 966, 1446, 1449, 2276
BlsI	GCNGC	9 cut(s)	176, 458, 490, 507, 510, 967, 1447, 1450, 2277
Bme1390I	CCNGG	4 cut(s)	112, 1390, 1935, 2064
Bme18I	GGWCC	4 cut(s)	57, 798, 1383, 2697
BmeRI	GACNNNNNGTC	1 cut(s)	2677
BmeT110I	CYCGRG	1 cut(s)	409
BmgT120I	GGNCC	6 cut(s)	57, 798, 1383, 1598, 2228, 2697
BmiI	GGNNCC	8 cut(s)	220, 395, 800, 833, 1484, 1814, 2239, 2699
BmrFI	CCNGG	4 cut(s)	112, 1390, 1935, 2064
BmsI	GCATC	7 cut(s)	452, 598, 696, 721, 2047, 2565, 2602
BoxI	GACNNNNGTC	1 cut(s)	870
BpiI	GAAGAC	4 cut(s)	599, 1904, 2165, 2471
BplI	GAGNNNNNCTC	4 cut(s)	504, 536, 2492, 2524
BpmI	CTGGAG	4 cut(s)	94, 818, 1884, 2046
Bpu10I	CCTNAGC	5 cut(s)	377, 1097, 1379, 2056, 2155
Bpu14I	TTCGAA	2 cut(s)	704, 2856
BpuEI	CTTGAG	2 cut(s)	342, 1897
Bsa29I	ATCGAT	1 cut(s)	204
BsaAI	YACGTR	2 cut(s)	1053, 1809
BsaBI	GATNNNNATC	1 cut(s)	79
BsaHI	GRCGYC	3 cut(s)	219, 239, 603
BsaI	GGTCTC	2 cut(s)	620, 1310
BsaJI	CCNNGG	9 cut(s)	14, 60, 111, 333, 1000, 1111, 1505, 1780, 2634
BsaWI	WCCGGW	2 cut(s)	1887, 2256
BsaXI	ACNNNNNCTCC	6 cut(s)	92, 122, 2044, 2074, 2778, 2808
Bsc4I	CCNNNNNNNGG	8 cut(s)	111, 273, 294, 378, 1297, 1374, 1707, 2160
Bse118I	RCCGGY	1 cut(s)	449
Bse1I	ACTGG	4 cut(s)	1618, 1720, 2111, 2792
Bse3DI	GCAATG	2 cut(s)	96, 1949
Bse8I	GATNNNNATC	1 cut(s)	79
BseBI	CCWGG	4 cut(s)	112, 1390, 1935, 2064
BseCI	ATCGAT	1 cut(s)	204
BseDI	CCNNGG	9 cut(s)	14, 60, 111, 333, 1000, 1111, 1505, 1780, 2634
BseGI	GGATG	4 cut(s)	10, 263, 1836, 2038
BseJI	GATNNNNATC	1 cut(s)	79
BseLI	CCNNNNNNNGG	8 cut(s)	111, 273, 294, 378, 1297, 1374, 1707, 2160
BseMI	GCAATG	2 cut(s)	96, 1949
BseNI	ACTGG	4 cut(s)	1618, 1720, 2111, 2792
BseRI	GAGGAG	3 cut(s)	394, 1141, 1198
BseSI	GKGCMC	3 cut(s)	1806, 2015, 2242
BseXI	GCAGC	5 cut(s)	186, 520, 977, 1432, 2262
BseYI	CCCAGC	4 cut(s)	1478, 1574, 2014, 2159
Bsh1236I	CGCG	2 cut(s)	495, 669
Bsh1285I	CGRYCG	2 cut(s)	526, 874
BshFI	GGCC	9 cut(s)	332, 338, 488, 657, 1301, 1451, 1600, 2230, 2713
BshNI	GGYRCC	3 cut(s)	218, 1812, 2237
BshVI	ATCGAT	1 cut(s)	204
BsiEI	CGRYCG	2 cut(s)	526, 874
BsiHKAI	GWGCWC	7 cut(s)	321, 366, 517, 786, 1151, 1806, 2791
BsiHKCI	CYCGRG	1 cut(s)	409
BsiSI	CCGG	7 cut(s)	72, 86, 222, 450, 537, 1888, 2257
BslFI	GGGAC	2 cut(s)	32, 784
BslI	CCNNNNNNNGG	8 cut(s)	111, 273, 294, 378, 1297, 1374, 1707, 2160
BsmAI	GTCTC	4 cut(s)	620, 1022, 1310, 2183
BsmBI	CGTCTC	1 cut(s)	1022
BsmFI	GGGAC	2 cut(s)	32, 784
BsmI	GAATGC	2 cut(s)	445, 759
BsnI	GGCC	9 cut(s)	332, 338, 488, 657, 1301, 1451, 1600, 2230, 2713
Bso31I	GGTCTC	2 cut(s)	620, 1310
BsoBI	CYCGRG	1 cut(s)	409
Bsp119I	TTCGAA	2 cut(s)	704, 2856
Bsp1407I	TGTACA	1 cut(s)	2005
Bsp19I	CCATGG	3 cut(s)	60, 1780, 2634
BspANI	GGCC	9 cut(s)	332, 338, 488, 657, 1301, 1451, 1600, 2230, 2713
BspDI	ATCGAT	1 cut(s)	204
BspFNI	CGCG	2 cut(s)	495, 669
BspLI	GGNNCC	8 cut(s)	220, 395, 800, 833, 1484, 1814, 2239, 2699
BspMI	ACCTGC	1 cut(s)	748
BspPI	GGATC	3 cut(s)	82, 123, 983
BspQI	GCTCTTC	2 cut(s)	1235, 2349
BspT104I	TTCGAA	2 cut(s)	704, 2856
BspT107I	GGYRCC	3 cut(s)	218, 1812, 2237
BspTI	CTTAAG	1 cut(s)	2120
BspTNI	GGTCTC	2 cut(s)	620, 1310
BsrBI	CCGCTC	1 cut(s)	491
BsrDI	GCAATG	2 cut(s)	96, 1949
BsrFI	RCCGGY	1 cut(s)	449
BsrGI	TGTACA	1 cut(s)	2005
BsrI	ACTGG	4 cut(s)	1618, 1720, 2111, 2792
BssAI	RCCGGY	1 cut(s)	449
BssECI	CCNNGG	9 cut(s)	14, 60, 111, 333, 1000, 1111, 1505, 1780, 2634
BssNI	GRCGYC	3 cut(s)	219, 239, 603
BssSI	CACGAG	3 cut(s)	20, 1084, 1799
BssT1I	CCWWGG	5 cut(s)	60, 1000, 1111, 1780, 2634
Bst2BI	CACGAG	3 cut(s)	20, 1084, 1799
Bst2UI	CCWGG	4 cut(s)	112, 1390, 1935, 2064
Bst4CI	ACNGT	9 cut(s)	193, 872, 1251, 1665, 1906, 1974, 1995, 2010, 2756
Bst6I	CTCTTC	5 cut(s)	668, 975, 1235, 2159, 2349
BstACI	GRCGYC	3 cut(s)	219, 239, 603
BstAFI	CTTAAG	1 cut(s)	2120
BstAUI	TGTACA	1 cut(s)	2005
BstBAI	YACGTR	2 cut(s)	1053, 1809
BstBI	TTCGAA	2 cut(s)	704, 2856
BstC8I	GCNNGC	9 cut(s)	217, 486, 493, 513, 645, 1312, 1656, 2361, 2816
BstDSI	CCRYGG	4 cut(s)	60, 333, 1780, 2634
BstENI	CCTNNNNNAGG	1 cut(s)	1705
BstF5I	GGATG	4 cut(s)	10, 263, 1836, 2038
BstFNI	CGCG	2 cut(s)	495, 669
BstH2I	RGCGCY	6 cut(s)	28, 72, 222, 485, 543, 554
BstHHI	GCGC	9 cut(s)	27, 71, 221, 484, 497, 542, 553, 671, 1228
BstMAI	GTCTC	4 cut(s)	620, 1022, 1310, 2183
BstMCI	CGRYCG	2 cut(s)	526, 874
BstNI	CCWGG	4 cut(s)	112, 1390, 1935, 2064
BstNSI	RCATGY	1 cut(s)	2002
BstPAI	GACNNNNGTC	1 cut(s)	870
BstSCI	CCNGG	4 cut(s)	110, 1388, 1933, 2062
BstSFI	CTRYAG	1 cut(s)	1063
BstSLI	GKGCMC	3 cut(s)	1806, 2015, 2242
BstSNI	TACGTA	1 cut(s)	1053
BstUI	CGCG	2 cut(s)	495, 669
BstV1I	GCAGC	5 cut(s)	186, 520, 977, 1432, 2262
BstV2I	GAAGAC	4 cut(s)	599, 1904, 2165, 2471
BstX2I	RGATCY	2 cut(s)	837, 988
BstYI	RGATCY	2 cut(s)	837, 988
Bsu15I	ATCGAT	1 cut(s)	204
BsuI	GTATCC	1 cut(s)	291
BsuRI	GGCC	9 cut(s)	332, 338, 488, 657, 1301, 1451, 1600, 2230, 2713
BsuTUI	ATCGAT	1 cut(s)	204
BtgI	CCRYGG	4 cut(s)	60, 333, 1780, 2634
BtgZI	GCGATG	1 cut(s)	2082
BtsCI	GGATG	4 cut(s)	10, 263, 1836, 2038
BtsIMutI	CAGTG	3 cut(s)	1713, 2015, 2752
BveI	ACCTGC	1 cut(s)	748
Cac8I	GCNNGC	9 cut(s)	217, 486, 493, 513, 645, 1312, 1656, 2361, 2816
CaiI	CAGNNNCTG	1 cut(s)	2369
CfoI	GCGC	9 cut(s)	27, 71, 221, 484, 497, 542, 553, 671, 1228
Cfr10I	RCCGGY	1 cut(s)	449
Cfr13I	GGNCC	6 cut(s)	57, 798, 1383, 1598, 2228, 2697
ClaI	ATCGAT	1 cut(s)	204
CseI	GACGC	5 cut(s)	166, 592, 756, 1039, 2119
Csp6I	GTAC	9 cut(s)	588, 1054, 1252, 1406, 1813, 1981, 1996, 2006, 2599
CspCI	CAANNNNNGTGG	2 cut(s)	1375, 1410
CviQI	GTAC	9 cut(s)	588, 1054, 1252, 1406, 1813, 1981, 1996, 2006, 2599
DinI	GGCGCC	1 cut(s)	220
DraI	TTTAAA	1 cut(s)	2322
DriI	GACNNNNNGTC	1 cut(s)	2677
EaeI	YGGCCR	5 cut(s)	336, 486, 655, 1299, 1449
Eam1104I	CTCTTC	5 cut(s)	668, 975, 1235, 2159, 2349
Eam1105I	GACNNNNNGTC	1 cut(s)	2677
EarI	CTCTTC	5 cut(s)	668, 975, 1235, 2159, 2349
EciI	GGCGGA	1 cut(s)	474
Ecl136II	GAGCTC	3 cut(s)	319, 364, 784
Eco105I	TACGTA	1 cut(s)	1053
Eco130I	CCWWGG	5 cut(s)	60, 1000, 1111, 1780, 2634
Eco147I	AGGCCT	1 cut(s)	2713
Eco24I	GRGCYC	5 cut(s)	10, 186, 321, 366, 786
Eco31I	GGTCTC	2 cut(s)	620, 1310
Eco47I	GGWCC	4 cut(s)	57, 798, 1383, 2697
Eco47III	AGCGCT	1 cut(s)	552
Eco53kI	GAGCTC	3 cut(s)	319, 364, 784
Eco57I	CTGAAG	3 cut(s)	231, 1937, 2483
Eco72I	CACGTG	1 cut(s)	1809
Eco88I	CYCGRG	1 cut(s)	409
EcoICRI	GAGCTC	3 cut(s)	319, 364, 784
EcoNI	CCTNNNNNAGG	1 cut(s)	1705
EcoRI	GAATTC	1 cut(s)	2858
EcoRII	CCWGG	4 cut(s)	110, 1388, 1933, 2062
EcoT14I	CCWWGG	5 cut(s)	60, 1000, 1111, 1780, 2634
EcoT22I	ATGCAT	3 cut(s)	445, 689, 1637
EcoT38I	GRGCYC	5 cut(s)	10, 186, 321, 366, 786
EgeI	GGCGCC	1 cut(s)	220
EheI	GGCGCC	1 cut(s)	220
ErhI	CCWWGG	5 cut(s)	60, 1000, 1111, 1780, 2634
Esp3I	CGTCTC	1 cut(s)	1022
FalI	AAGNNNNNCTT	1 cut(s)	2858
FaqI	GGGAC	2 cut(s)	32, 784
FauNDI	CATATG	1 cut(s)	1235
FbaI	TGATCA	2 cut(s)	972, 2554
FblI	GTMKAC	2 cut(s)	745, 1533
Fnu4HI	GCNGC	9 cut(s)	175, 457, 489, 506, 509, 966, 1446, 1449, 2276
FokI	GGATG	3 cut(s)	250, 1843, 2025
FriOI	GRGCYC	5 cut(s)	10, 186, 321, 366, 786
Fsp4HI	GCNGC	9 cut(s)	175, 457, 489, 506, 509, 966, 1446, 1449, 2276
FspBI	CTAG	2 cut(s)	1583, 2592
GlaI	GCGC	9 cut(s)	26, 70, 220, 483, 496, 541, 552, 670, 1227
GluI	GCNGC	9 cut(s)	175, 457, 489, 506, 509, 966, 1446, 1449, 2276
GsaI	CCCAGC	4 cut(s)	1482, 1578, 2018, 2163
GsuI	CTGGAG	4 cut(s)	94, 818, 1884, 2046
HaeII	RGCGCY	6 cut(s)	28, 72, 222, 485, 543, 554
HaeIII	GGCC	9 cut(s)	332, 338, 488, 657, 1301, 1451, 1600, 2230, 2713
HapII	CCGG	7 cut(s)	72, 86, 222, 450, 537, 1888, 2257
HgaI	GACGC	5 cut(s)	166, 592, 756, 1039, 2119
HhaI	GCGC	9 cut(s)	27, 71, 221, 484, 497, 542, 553, 671, 1228
Hin1I	GRCGYC	3 cut(s)	219, 239, 603
Hin6I	GCGC	9 cut(s)	25, 69, 219, 482, 495, 540, 551, 669, 1226
HinP1I	GCGC	9 cut(s)	25, 69, 219, 482, 495, 540, 551, 669, 1226
HindIII	AAGCTT	3 cut(s)	1688, 2139, 2213
HpaII	CCGG	7 cut(s)	72, 86, 222, 450, 537, 1888, 2257
HphI	GGTGA	3 cut(s)	2114, 2621, 2623
Hpy166II	GTNNAC	9 cut(s)	746, 856, 1386, 1534, 1804, 1991, 2086, 2599, 2671
Hpy8I	GTNNAC	9 cut(s)	746, 856, 1386, 1534, 1804, 1991, 2086, 2599, 2671
Hpy99I	CGWCG	7 cut(s)	101, 131, 182, 241, 244, 608, 872
HpyAV	CCTTC	8 cut(s)	937, 1116, 1301, 1711, 1738, 1912, 2160, 2600
HpyCH4III	ACNGT	9 cut(s)	193, 872, 1251, 1665, 1906, 1974, 1995, 2010, 2756
HpyCH4IV	ACGT	4 cut(s)	239, 1052, 1808, 1941
HpySE526I	ACGT	4 cut(s)	239, 1052, 1808, 1941
Hsp92I	GRCGYC	3 cut(s)	219, 239, 603
HspAI	GCGC	9 cut(s)	25, 69, 219, 482, 495, 540, 551, 669, 1226
KasI	GGCGCC	1 cut(s)	218
KpnI	GGTACC	1 cut(s)	1816
Ksp22I	TGATCA	2 cut(s)	972, 2554
LguI	GCTCTTC	2 cut(s)	1235, 2349
LmnI	GCTCC	7 cut(s)	316, 393, 539, 837, 2065, 2500, 2786
Lsp1109I	GCAGC	5 cut(s)	186, 520, 977, 1432, 2262
LweI	GCATC	7 cut(s)	452, 598, 696, 721, 2047, 2565, 2602
MaeI	CTAG	2 cut(s)	1583, 2592
MaeII	ACGT	4 cut(s)	239, 1052, 1808, 1941
MaeIII	GTNAC	5 cut(s)	133, 575, 1676, 2102, 2491
MbiI	CCGCTC	1 cut(s)	491
MfeI	CAATTG	2 cut(s)	1022, 1440
MflI	RGATCY	2 cut(s)	837, 988
MlsI	TGGCCA	1 cut(s)	338
MluNI	TGGCCA	1 cut(s)	338
Mly113I	GGCGCC	1 cut(s)	219
MlyI	GAGTC	4 cut(s)	35, 95, 1138, 1197
MmeI	TCCRAC	4 cut(s)	129, 260, 312, 699
Mox20I	TGGCCA	1 cut(s)	338
Mph1103I	ATGCAT	3 cut(s)	445, 689, 1637
MroXI	GAANNNNTTC	1 cut(s)	2729
MscI	TGGCCA	1 cut(s)	338
MseI	TTAA	8 cut(s)	563, 1157, 1593, 1686, 1977, 2121, 2219, 2321
MslI	CAYNNNNRTG	6 cut(s)	204, 692, 2573, 2633, 2690, 2757
Msp20I	TGGCCA	1 cut(s)	338
MspCI	CTTAAG	1 cut(s)	2120
MspI	CCGG	7 cut(s)	72, 86, 222, 450, 537, 1888, 2257
MspR9I	CCNGG	4 cut(s)	112, 1390, 1935, 2064
MunI	CAATTG	2 cut(s)	1022, 1440
Mva1269I	GAATGC	2 cut(s)	445, 759
MvaI	CCWGG	4 cut(s)	112, 1390, 1935, 2064
MvnI	CGCG	2 cut(s)	495, 669
NarI	GGCGCC	1 cut(s)	219
NcoI	CCATGG	3 cut(s)	60, 1780, 2634
NdeI	CATATG	1 cut(s)	1235
NlaIV	GGNNCC	8 cut(s)	220, 395, 800, 833, 1484, 1814, 2239, 2699
NmeAIII	GCCGAG	2 cut(s)	397, 1354
NmuCI	GTSAC	1 cut(s)	2102
NsiI	ATGCAT	3 cut(s)	445, 689, 1637
NspI	RCATGY	1 cut(s)	2002
NspV	TTCGAA	2 cut(s)	704, 2856
OliI	CACNNNNGTG	1 cut(s)	2690
PaeR7I	CTCGAG	1 cut(s)	409
PceI	AGGCCT	1 cut(s)	2713
PciI	ACATGT	1 cut(s)	1998
PciSI	GCTCTTC	2 cut(s)	1235, 2349
PcsI	WCGNNNNNNNCGW	1 cut(s)	266
PctI	GAATGC	2 cut(s)	445, 759
PdmI	GAANNNNTTC	1 cut(s)	2729
PfeI	GAWTC	8 cut(s)	701, 824, 1526, 1872, 1881, 2292, 2366, 2417
PflFI	GACNNNGTC	1 cut(s)	44
PfoI	TCCNGGA	1 cut(s)	1933
PkrI	GCNGC	9 cut(s)	176, 458, 490, 507, 510, 967, 1447, 1450, 2277
Ple19I	CGATCG	1 cut(s)	526
PleI	GAGTC	4 cut(s)	35, 95, 1137, 1196
PluTI	GGCGCC	1 cut(s)	222
PmaCI	CACGTG	1 cut(s)	1809
PmlI	CACGTG	1 cut(s)	1809
PpsI	GAGTC	4 cut(s)	35, 95, 1137, 1196
Ppu21I	YACGTR	2 cut(s)	1053, 1809
PscI	ACATGT	1 cut(s)	1998
PshAI	GACNNNNGTC	1 cut(s)	870
PsiI	TTATAA	2 cut(s)	1514, 1622
Psp124BI	GAGCTC	3 cut(s)	321, 366, 786
Psp1406I	AACGTT	1 cut(s)	1941
Psp6I	CCWGG	4 cut(s)	110, 1388, 1933, 2062
PspCI	CACGTG	1 cut(s)	1809
PspFI	CCCAGC	4 cut(s)	1478, 1574, 2014, 2159
PspGI	CCWGG	4 cut(s)	110, 1388, 1933, 2062
PspN4I	GGNNCC	8 cut(s)	220, 395, 800, 833, 1484, 1814, 2239, 2699
PspPI	GGNCC	6 cut(s)	57, 798, 1383, 1598, 2228, 2697
PstNI	CAGNNNCTG	1 cut(s)	2369
PsuI	RGATCY	2 cut(s)	837, 988
PsyI	GACNNNGTC	1 cut(s)	44
PvuI	CGATCG	1 cut(s)	526
RsaI	GTAC	9 cut(s)	589, 1055, 1253, 1407, 1814, 1982, 1997, 2007, 2600
RsaNI	GTAC	9 cut(s)	588, 1054, 1252, 1406, 1813, 1981, 1996, 2006, 2599
RseI	CAYNNNNRTG	6 cut(s)	204, 692, 2573, 2633, 2690, 2757
SacI	GAGCTC	3 cut(s)	321, 366, 786
SapI	GCTCTTC	2 cut(s)	1235, 2349
SaqAI	TTAA	8 cut(s)	563, 1157, 1593, 1686, 1977, 2121, 2219, 2321
SatI	GCNGC	9 cut(s)	175, 457, 489, 506, 509, 966, 1446, 1449, 2276
Sau96I	GGNCC	6 cut(s)	57, 798, 1383, 1598, 2228, 2697
SchI	GAGTC	4 cut(s)	35, 95, 1138, 1197
ScrFI	CCNGG	4 cut(s)	112, 1390, 1935, 2064
SfaNI	GCATC	7 cut(s)	452, 598, 696, 721, 2047, 2565, 2602
SfcI	CTRYAG	1 cut(s)	1063
SfoI	GGCGCC	1 cut(s)	220
Sfr274I	CTCGAG	1 cut(s)	409
SfuI	TTCGAA	2 cut(s)	704, 2856
SinI	GGWCC	4 cut(s)	57, 798, 1383, 2697
SlaI	CTCGAG	1 cut(s)	409
SmiMI	CAYNNNNRTG	6 cut(s)	204, 692, 2573, 2633, 2690, 2757
SmlI	CTYRAG	4 cut(s)	357, 409, 1876, 2120
SmoI	CTYRAG	4 cut(s)	357, 409, 1876, 2120
SnaBI	TACGTA	1 cut(s)	1053
SpeI	ACTAGT	1 cut(s)	2591
SseBI	AGGCCT	1 cut(s)	2713
SspDI	GGCGCC	1 cut(s)	218
SspI	AATATT	1 cut(s)	2341
SspMI	CTAG	2 cut(s)	1583, 2592
SstI	GAGCTC	3 cut(s)	321, 366, 786
StuI	AGGCCT	1 cut(s)	2713
StyD4I	CCNGG	4 cut(s)	110, 1388, 1933, 2062
StyI	CCWWGG	5 cut(s)	60, 1000, 1111, 1780, 2634
TaaI	ACNGT	9 cut(s)	193, 872, 1251, 1665, 1906, 1974, 1995, 2010, 2756
TaiI	ACGT	4 cut(s)	242, 1055, 1811, 1944
TaqII	GACCGA	1 cut(s)	110
TatI	WGTACW	4 cut(s)	1251, 1980, 1995, 2005
TauI	GCSGC	4 cut(s)	459, 491, 508, 1451
TfiI	GAWTC	8 cut(s)	701, 824, 1526, 1872, 1881, 2292, 2366, 2417
Tru1I	TTAA	8 cut(s)	563, 1157, 1593, 1686, 1977, 2121, 2219, 2321
Tru9I	TTAA	8 cut(s)	563, 1157, 1593, 1686, 1977, 2121, 2219, 2321
TscAI	CASTG	3 cut(s)	1720, 2015, 2759
TseFI	GTSAC	1 cut(s)	2102
TseI	GCWGC	5 cut(s)	174, 508, 965, 1445, 2275
Tsp45I	GTSAC	1 cut(s)	2102
TspDTI	ATGAA	7 cut(s)	243, 559, 1222, 1234, 2299, 2549, 2553
TspGWI	ACGGA	4 cut(s)	34, 289, 1898, 2016
TspRI	CASTG	3 cut(s)	1720, 2015, 2759
Tth111I	GACNNNGTC	1 cut(s)	44
Vha464I	CTTAAG	1 cut(s)	2120
VneI	GTGCAC	1 cut(s)	1802
VpaK11BI	GGWCC	4 cut(s)	57, 798, 1383, 2697
XagI	CCTNNNNNAGG	1 cut(s)	1705
XapI	RAATTY	4 cut(s)	804, 1241, 1325, 2858
XbaI	TCTAGA	1 cut(s)	1582
XceI	RCATGY	1 cut(s)	2002
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2660
XhoI	CTCGAG	1 cut(s)	409
XmiI	GTMKAC	2 cut(s)	745, 1533
XmnI	GAANNNNTTC	1 cut(s)	2729
XspI	CTAG	2 cut(s)	1583, 2592
ZraI	GACGTC	1 cut(s)	240
Zsp2I	ATGCAT	3 cut(s)	445, 689, 1637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.