Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0009819

Physical Location & Seq

Genomic Coordinates Forward (+)

53718301 .. 53726446

Gene Structure 8146 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00259290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2580 bp

ATGGGCCTCGGACTCTGTCCCAGAGAAGACGCTAGTCAGCACTGGCACGTCCCCGGGACTGAGTCTACCTCGCCGGAGGAGTTCGTCGAGGTGACTCTAGATTTGCAGGACGACAACACCATCGTCCTCCGCAGCGTCGAGCCGGCGACGGTGATTAATATTACAGACGATGGAGGAGCCGAGGCTACTCCGGCGTCTTCCTCGAGGTCGCCGTCGATGAGGCGGAGCTCGTCCAACAGCAGAATCCGGCAGTTCTCTCAAGAGCTGAAAGCTGAGGCGGTGGCCAAGGCCAAGCAGTTCTCTCAGGAGCTCAAGGCTGAGCTGAGGCGGTTCTCGTGGAGCCATGGAAATGCGTCGCGCGTGCTGGCGGCTTCTTCGGCAGTTGCTTCTCAGAATGACGGCGGAGGCGGAGGAGGAGGAGCCGGGAGTGGTTATTATGACTCGGCTCTGGCGGCGAGGGCTCTGAGGAGGCAGCGGGCTCAGCTAGATCGGACCCGCTCCGGAGCGCAGAAGGCCCTCCGGGGACTGAGGTTCATCAGCAACTGCAAGAACAAGAGCAATGGCGTGGATGCATGGAACGAGGTGGAGAGCAATTTCAACAAGCTCGCCAAAGACGGCAACCTCTACCGCTCCGATTTCGCCCAATGCATAGGAATGAAAGATTCGAAGGAGTTTGCTCTGGAACTGTTCGATGCGCTGGGCCGGAGACGAAGACTCAAGGTGGAGAAAATCAGCAAGGACGAGCTTTACGAGGTCGACAAAAATGAAGATGGAAGAATCACCGAAGAAGAAGTAAAGGAGATCATCATGCTGAGTGCTTGTGCCAACAAGTTATCAAGATTGAAGGAACAAGCAGAGGAATATGCAGCCCTGATCATGGAAGAGTTGGATCCTGAAAGACTTGGCTATATTGAGCTATGGCAATTGGAGACCCTTTTGCTCCAAAAGGACACGTACCTAAACTACAGCCAAGCACTGAGCTACACGAGCCAAGCCTTAAGCCAGAACCTCCAAGGCCTCCGAAGGCGAAGCCCGATCCGCAGAATCAGCAGCAAGTGCCTCTACTACTTGCAAGAAAACTGGAAGAGACTCTGGGTTTTGGCACTCTGGGTGTGCATTATGATCGGGCTTTTTACGTGGAAGTTCTTTCAGTACAAGCAGAAAAAATCCTTTCATATAATGGGGTACTGTCTTCTCACCGCCAAGGGTGCGGCAGAGACGCTGAAGTTCAACATGGCTCTGGTGCTGTTACCGGTGTGTAGAAACACCATTACTTGGCTCAGGAACACCAGGCTCGGGTTTGTTGTGCCTTTCGATGACAATATCAACTTCCACAAGAGCATTGCTGCAGCCATTGTAATCGGTGTTATTCTCCATGCTGGGAACCATCTTGCGTGTGATTTTCCGAGGCTTATAAAGGTGTCTGAATCAGTCTATGAGGAGTATTTGCATGACGACTTTGGAACGCATAAACCCACGTACGTAGATTTGGTTAAAGGGGCCGAAGGAGTGACTGGAATTATAATGGTGACTTGGATGTATCTTTCCGTTCCAGTTATACTATATGCTGGTGAAAGAATCCTGAGAGTCTTCCGGTCTGGCTTTTATACTGTCCGTCTTCTGAAGGTTGCTATTTATCCGGGAAATGTTCTCACATTGCAAATGTCTAAGCCACCCCAGTTTAAATACAAAAGTGGACAGTACATGTTTGTTCAGTGCCCAGCTGTTTCCCCTTTTGAGTGGCATCCGTTTTCAATTACATCTGCTCCCGGTGATGACTACCTTAGCGTTCACATCCGTCAGCTTGGTGACTGGACACAAGAGCTTAAGAGGGTTTTCTCAGAAGCCTGTGAGCCTCCCTTAGCTGGGAAGAGTGGCCTGCTAAGGGCTGATGAAACCACCAAGAAAAGTTTGCCCAAGCTTTTAATAGATGGACCTTATGGTGCCCCGGCACAAGACTACCGGAAATATGATGTCCTGCTACTTGTTGGTCTTGGGATTGGGGCGACACCTTTCATTAGTATTTTGAAAGATTTGCTCAACAATATTGTCAAAATGGAGGAACAGGCAGATTCTCCCTCAGATTTCAGTAGAAATTCAGACCTAAGTTCAGGAAGTACAGATTCTCCCAACAAGGTTTATCTGAAACGAAAAAAGCCACTGAAGACCACCAATGCCTACTTCTATTGGGTAACTAGGGAGCAAGGCTCATTTGATTGGTTCAAAGGTGTGATGAACGAAGTTGCAGATCTTGATCAACGGGGTGTCATTGAGATGCACAACTATTTGACTAGTGTGTATGAGGAAGGCGATGCTCGATCAGCGCTCATCACCATGGTCCAAGCTCTCAACCATGCTAAGAATGGGGTTGACATTGTATCTGGCACTAGGGTTCGCACTCATTTTGCAAGGCCTAACTGGAAGAAAGTTTTCTCAAAATTTTCCTCCAAGCACTATGGTGCAAGGATAGGGGTATTCTATTGTGGAGCACCAGTTTTGGCCAAAGAACTCAGCAAGCTTTGCTATGAGTTCAATCAAAAAGGTCTAACCAAATTTGAATTCCACAAGGAGCACTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0001101 GO:0002252 GO:0002376 GO:0002679 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009873 GO:0009987 GO:0010033 GO:0010119 GO:0010941 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0016999 GO:0017000 GO:0017144 GO:0023052 GO:0032870 GO:0033036 GO:0033037 GO:0033500 GO:0033554 GO:0033993 GO:0035556 GO:0042221 GO:0042592 GO:0042743 GO:0043067 GO:0043069 GO:0044237 GO:0044249 GO:0044464 GO:0045730 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050665 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051716 GO:0052542 GO:0052545 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071369 GO:0071396 GO:0071470 GO:0071495 GO:0071944 GO:0072593 GO:0097305 GO:0097306 GO:0104004 GO:1901700 GO:1901701 GO:1903409

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

859

Amino Acids

96.91

Weight (kDa)

9.0

Isoelectric Point (pI)

53.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	161 - 252	3.1e-32	Respiratory burst NADPH oxidase
FAD_binding_8	PF08022	536 - 651	1.4e-36	FAD-binding domain
NAD_binding_6	PF08030	657 - 841	6.1e-52	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64060
fragaria_vesca	FvH4_5g03310 FvH4_5g03310 FvH4_5g03310 FvH4_5g03310
malus_domestica	MD06G1093000.v1.1 MD14G1113700.v1.1
prunus_persica	Prupe.5G107400_v2.0.a1
pyrus_communis	pycom06g08880
rosa_chinensis	RchiOBHm_Chr7g0196471
rosa_laevigata	RLG00000004012
rosa_multiflora	Rmu_co8333625.1_g000001 Rmu_sc0000736.1_g000003
rosa_roxburghii	Rroxscaffold_3G00259290
rosa_rugosa	Rorug07G0033600 Rorug07G0033700
rosa_samantha	Rh7AG158000 Rh7BG159900 Rh7CG166400 Rh7DG159200
rosa_wichuraiana	Rw7G013810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1415, 1523
AbsI	CCTCGAGG	1 cut(s)	202
AccB1I	GGYRCC	1 cut(s)	1943
AccB7I	CCANNNNNTGG	1 cut(s)	1275
AccBSI	CCGCTC	2 cut(s)	498, 630
AccI	GTMKAC	2 cut(s)	65, 756
AccII	CGCG	2 cut(s)	358, 360
AccIII	TCCGGA	1 cut(s)	500
AclWI	GGATC	3 cut(s)	884, 897, 1030
AcoI	YGGCCR	2 cut(s)	282, 2499
AcsI	RAATTY	4 cut(s)	2095, 2438, 2552, 2558
AcuI	CTGAAG	3 cut(s)	1244, 1643, 2183
AcyI	GRCGYC	1 cut(s)	194
AfaI	GTAC	6 cut(s)	956, 1154, 1187, 1481, 1703, 2119
AfeI	AGCGCT	1 cut(s)	2325
AfiI	CCNNNNNNNGG	6 cut(s)	877, 1275, 1296, 1865, 1866, 1885
AflII	CTTAAG	2 cut(s)	997, 1826
AflIII	ACRYGT	2 cut(s)	951, 1704
AgeI	ACCGGT	1 cut(s)	1252
AgsI	TTSAA	8 cut(s)	598, 844, 1231, 1755, 2029, 2224, 2533, 2558
AhlI	ACTAGT	1 cut(s)	2291
AjiI	CACGTC	1 cut(s)	49
AjnI	CCWGG	1 cut(s)	1289
AjuI	GAANNNNNNNTTGG	4 cut(s)	2165, 2197, 2543, 2575
AleI	CACNNNNGTG	1 cut(s)	2457
Alw21I	GWGCWC	4 cut(s)	230, 312, 2491, 2574
Alw26I	GTCTC	4 cut(s)	700, 923, 1081, 1211
AlwI	GGATC	3 cut(s)	884, 897, 1030
AlwNI	CAGNNNCTG	1 cut(s)	543
Ama87I	CYCGRG	3 cut(s)	53, 202, 1295
Aor13HI	TCCGGA	1 cut(s)	500
Aor51HI	AGCGCT	1 cut(s)	2325
ApeKI	GCWGC	6 cut(s)	132, 472, 866, 1050, 1346, 1349
ApoI	RAATTY	4 cut(s)	2095, 2438, 2552, 2558
ArsI	GACNNNNNNTTYG	2 cut(s)	85, 117
AseI	ATTAAT	1 cut(s)	156
AsiGI	ACCGGT	1 cut(s)	1252
Asp700I	GAANNNNTTC	1 cut(s)	2429
AspLEI	GCGC	4 cut(s)	360, 508, 697, 2326
AspS9I	GGNCC	7 cut(s)	4, 492, 514, 700, 1500, 1934, 2338
AsuC2I	CCSGG	7 cut(s)	54, 55, 424, 521, 1641, 1770, 1949
AsuHPI	GGTGA	9 cut(s)	103, 163, 772, 1189, 1540, 1583, 1784, 1820, 2323
AsuII	TTCGAA	1 cut(s)	665
AvaI	CYCGRG	3 cut(s)	53, 202, 1295
AvaII	GGWCC	3 cut(s)	492, 1934, 2338
BaeGI	GKGCMC	2 cut(s)	1721, 1948
BalI	TGGCCA	2 cut(s)	284, 2501
BamHI	GGATCC	1 cut(s)	889
BanI	GGYRCC	1 cut(s)	1943
BanII	GRGCYC	4 cut(s)	230, 312, 463, 481
BauI	CACGAG	2 cut(s)	334, 985
BbsI	GAAGAC	7 cut(s)	33, 189, 718, 1184, 1582, 1610, 2171
Bbv12I	GWGCWC	4 cut(s)	230, 312, 2491, 2574
BbvCI	CCTCAGC	2 cut(s)	273, 323
BbvI	GCAGC	6 cut(s)	144, 484, 878, 1062, 1333, 1361
BccI	CCATC	5 cut(s)	128, 164, 764, 1395, 1925
BceAI	ACGGC	3 cut(s)	196, 415, 631
BcgI	CGANNNNNNTGC	2 cut(s)	1105, 1139
BciT130I	CCWGG	1 cut(s)	1291
BclI	TGATCA	2 cut(s)	873, 2254
BcnI	CCSGG	7 cut(s)	54, 55, 424, 521, 1641, 1770, 1949
BcoDI	GTCTC	4 cut(s)	700, 923, 1081, 1211
BcuI	ACTAGT	1 cut(s)	2291
BfaI	CTAG	6 cut(s)	33, 98, 485, 2196, 2292, 2388
BfmI	CTRYAG	2 cut(s)	964, 1347
BfoI	RGCGCY	1 cut(s)	2327
BfrI	CTTAAG	2 cut(s)	997, 1826
BglII	AGATCT	1 cut(s)	2248
BisI	GCNGC	9 cut(s)	133, 369, 453, 473, 867, 1051, 1212, 1347, 1350
BlpI	GCTNAGC	2 cut(s)	318, 480
BlsI	GCNGC	9 cut(s)	134, 370, 454, 474, 868, 1052, 1213, 1348, 1351
Bme1390I	CCNGG	8 cut(s)	54, 55, 424, 521, 1291, 1641, 1770, 1949
Bme18I	GGWCC	3 cut(s)	492, 1934, 2338
BmeT110I	CYCGRG	3 cut(s)	53, 202, 1295
BmgBI	CACGTC	1 cut(s)	49
BmgT120I	GGNCC	7 cut(s)	4, 492, 514, 700, 1500, 1934, 2338
BmiI	GGNNCC	8 cut(s)	178, 341, 421, 494, 891, 1385, 1501, 1945
BmrFI	CCNGG	8 cut(s)	54, 55, 424, 521, 1291, 1641, 1770, 1949
BmrI	ACTGGG	1 cut(s)	1672
BmsI	GCATC	5 cut(s)	559, 682, 1753, 2265, 2302
BmuI	ACTGGG	1 cut(s)	1672
BoxI	GACNNNNGTC	1 cut(s)	33
BpiI	GAAGAC	7 cut(s)	33, 189, 718, 1184, 1582, 1610, 2171
BplI	GAGNNNNNCTC	4 cut(s)	53, 85, 2192, 2224
Bpu10I	CCTNAGC	6 cut(s)	273, 323, 1280, 1784, 1861, 1883
Bpu1102I	GCTNAGC	2 cut(s)	318, 480
Bpu14I	TTCGAA	1 cut(s)	665
BpuEI	CTTGAG	3 cut(s)	243, 296, 701
BpuMI	CCSGG	7 cut(s)	54, 55, 424, 521, 1641, 1770, 1949
BsaAI	YACGTR	4 cut(s)	954, 1137, 1479, 1483
BsaBI	GATNNNNATC	1 cut(s)	2253
BsaHI	GRCGYC	1 cut(s)	194
BsaI	GGTCTC	1 cut(s)	923
BsaWI	WCCGGW	4 cut(s)	500, 1252, 1593, 1962
BsaXI	ACNNNNNCTCC	6 cut(s)	1750, 1780, 2110, 2140, 2478, 2508
Bsc4I	CCNNNNNNNGG	6 cut(s)	877, 1275, 1296, 1865, 1866, 1885
Bse118I	RCCGGY	2 cut(s)	142, 1252
Bse1I	ACTGG	8 cut(s)	47, 1085, 1519, 1553, 1678, 1817, 2423, 2492
Bse3DI	GCAATG	3 cut(s)	565, 1341, 1655
Bse8I	GATNNNNATC	1 cut(s)	2253
BseAI	TCCGGA	1 cut(s)	500
BseBI	CCWGG	1 cut(s)	1291
BseGI	GGATG	4 cut(s)	574, 1542, 1744, 1794
BseJI	GATNNNNATC	1 cut(s)	2253
BseLI	CCNNNNNNNGG	6 cut(s)	877, 1275, 1296, 1865, 1866, 1885
BseMI	GCAATG	3 cut(s)	565, 1341, 1655
BseNI	ACTGG	8 cut(s)	47, 1085, 1519, 1553, 1678, 1817, 2423, 2492
BseRI	GAGGAG	7 cut(s)	92, 189, 426, 429, 432, 481, 1454
BseSI	GKGCMC	2 cut(s)	1721, 1948
BseXI	GCAGC	6 cut(s)	144, 484, 878, 1062, 1333, 1361
BseYI	CCCAGC	4 cut(s)	697, 1379, 1720, 1865
Bsh1236I	CGCG	2 cut(s)	358, 360
BshNI	GGYRCC	1 cut(s)	1943
BshTI	ACCGGT	1 cut(s)	1252
BsiHKAI	GWGCWC	4 cut(s)	230, 312, 2491, 2574
BsiHKCI	CYCGRG	3 cut(s)	53, 202, 1295
BsiWI	CGTACG	1 cut(s)	1479
BslFI	GGGAC	4 cut(s)	3, 35, 70, 537
BslI	CCNNNNNNNGG	6 cut(s)	877, 1275, 1296, 1865, 1866, 1885
BsmAI	GTCTC	4 cut(s)	700, 923, 1081, 1211
BsmBI	CGTCTC	2 cut(s)	700, 1211
BsmFI	GGGAC	4 cut(s)	3, 35, 70, 537
Bso31I	GGTCTC	1 cut(s)	923
BsoBI	CYCGRG	3 cut(s)	53, 202, 1295
Bsp119I	TTCGAA	1 cut(s)	665
Bsp1286I	GDGCHC	8 cut(s)	230, 312, 463, 481, 1721, 1948, 2491, 2574
Bsp13I	TCCGGA	1 cut(s)	500
Bsp143I	GATC	9 cut(s)	487, 801, 873, 889, 1035, 1122, 2248, 2254, 2318
Bsp1720I	GCTNAGC	2 cut(s)	318, 480
Bsp19I	CCATGG	2 cut(s)	343, 2334
BspEI	TCCGGA	1 cut(s)	500
BspFNI	CGCG	2 cut(s)	358, 360
BspLI	GGNNCC	8 cut(s)	178, 341, 421, 494, 891, 1385, 1501, 1945
BspMAI	CTGCAG	1 cut(s)	1351
BspPI	GGATC	3 cut(s)	884, 897, 1030
BspT104I	TTCGAA	1 cut(s)	665
BspT107I	GGYRCC	1 cut(s)	1943
BspTI	CTTAAG	2 cut(s)	997, 1826
BspTNI	GGTCTC	1 cut(s)	923
BsrBI	CCGCTC	2 cut(s)	498, 630
BsrDI	GCAATG	3 cut(s)	565, 1341, 1655
BsrFI	RCCGGY	2 cut(s)	142, 1252
BsrI	ACTGG	8 cut(s)	47, 1085, 1519, 1553, 1678, 1817, 2423, 2492
BssAI	RCCGGY	2 cut(s)	142, 1252
BssMI	GATC	9 cut(s)	487, 801, 873, 889, 1035, 1122, 2248, 2254, 2318
BssNI	GRCGYC	1 cut(s)	194
BssSI	CACGAG	2 cut(s)	334, 985
BssT1I	CCWWGG	5 cut(s)	285, 343, 1012, 1203, 2334
Bst2BI	CACGAG	2 cut(s)	334, 985
Bst2UI	CCWGG	1 cut(s)	1291
Bst4CI	ACNGT	5 cut(s)	151, 687, 1190, 1612, 1701
Bst6I	CTCTTC	3 cut(s)	876, 1079, 1865
BstACI	GRCGYC	1 cut(s)	194
BstAFI	CTTAAG	2 cut(s)	997, 1826
BstAPI	GCANNNNNTGC	2 cut(s)	1056, 2520
BstBAI	YACGTR	4 cut(s)	954, 1137, 1479, 1483
BstBI	TTCGAA	1 cut(s)	665
BstC8I	GCNNGC	7 cut(s)	144, 362, 366, 477, 606, 1880, 2516
BstDSI	CCRYGG	2 cut(s)	343, 2334
BstF5I	GGATG	4 cut(s)	574, 1542, 1744, 1794
BstFNI	CGCG	2 cut(s)	358, 360
BstH2I	RGCGCY	1 cut(s)	2327
BstHHI	GCGC	4 cut(s)	360, 508, 697, 2326
BstKTI	GATC	9 cut(s)	490, 804, 876, 892, 1038, 1125, 2251, 2257, 2321
BstMAI	GTCTC	4 cut(s)	700, 923, 1081, 1211
BstMBI	GATC	9 cut(s)	487, 801, 873, 889, 1035, 1122, 2248, 2254, 2318
BstNI	CCWGG	1 cut(s)	1291
BstNSI	RCATGY	1 cut(s)	1708
BstPAI	GACNNNNGTC	1 cut(s)	33
BstSCI	CCNGG	8 cut(s)	52, 53, 422, 519, 1289, 1639, 1768, 1947
BstSFI	CTRYAG	2 cut(s)	964, 1347
BstSLI	GKGCMC	2 cut(s)	1721, 1948
BstSNI	TACGTA	1 cut(s)	1483
BstUI	CGCG	2 cut(s)	358, 360
BstV1I	GCAGC	6 cut(s)	144, 484, 878, 1062, 1333, 1361
BstV2I	GAAGAC	7 cut(s)	33, 189, 718, 1184, 1582, 1610, 2171
BstX2I	RGATCY	2 cut(s)	889, 2248
BstYI	RGATCY	2 cut(s)	889, 2248
BtgI	CCRYGG	2 cut(s)	343, 2334
BtgZI	GCGATG	1 cut(s)	2325
BtrI	CACGTC	1 cut(s)	49
BtsCI	GGATG	4 cut(s)	574, 1542, 1744, 1794
BtsIMutI	CAGTG	4 cut(s)	40, 974, 1721, 2159
Cac8I	GCNNGC	7 cut(s)	144, 362, 366, 477, 606, 1880, 2516
CaiI	CAGNNNCTG	1 cut(s)	543
CfoI	GCGC	4 cut(s)	360, 508, 697, 2326
Cfr10I	RCCGGY	2 cut(s)	142, 1252
Cfr13I	GGNCC	7 cut(s)	4, 492, 514, 700, 1500, 1934, 2338
Cfr9I	CCCGGG	1 cut(s)	53
CseI	GACGC	5 cut(s)	38, 124, 183, 342, 1228
Csp6I	GTAC	6 cut(s)	955, 1153, 1186, 1480, 1702, 2118
CspAI	ACCGGT	1 cut(s)	1252
CviQI	GTAC	6 cut(s)	955, 1153, 1186, 1480, 1702, 2118
DpnI	GATC	9 cut(s)	489, 803, 875, 891, 1037, 1124, 2250, 2256, 2320
DpnII	GATC	9 cut(s)	487, 801, 873, 889, 1035, 1122, 2248, 2254, 2318
DraI	TTTAAA	1 cut(s)	1684
EaeI	YGGCCR	2 cut(s)	282, 2499
Eam1104I	CTCTTC	3 cut(s)	876, 1079, 1865
EarI	CTCTTC	3 cut(s)	876, 1079, 1865
EciI	GGCGGA	3 cut(s)	238, 417, 423
Ecl136II	GAGCTC	2 cut(s)	228, 310
Eco105I	TACGTA	1 cut(s)	1483
Eco130I	CCWWGG	5 cut(s)	285, 343, 1012, 1203, 2334
Eco147I	AGGCCT	2 cut(s)	1017, 2413
Eco24I	GRGCYC	4 cut(s)	230, 312, 463, 481
Eco31I	GGTCTC	1 cut(s)	923
Eco47I	GGWCC	3 cut(s)	492, 1934, 2338
Eco47III	AGCGCT	1 cut(s)	2325
Eco53kI	GAGCTC	2 cut(s)	228, 310
Eco57I	CTGAAG	3 cut(s)	1244, 1643, 2183
Eco88I	CYCGRG	3 cut(s)	53, 202, 1295
EcoICRI	GAGCTC	2 cut(s)	228, 310
EcoO109I	RGGNCCY	1 cut(s)	514
EcoRI	GAATTC	1 cut(s)	2558
EcoRII	CCWGG	1 cut(s)	1289
EcoT14I	CCWWGG	5 cut(s)	285, 343, 1012, 1203, 2334
EcoT22I	ATGCAT	2 cut(s)	574, 650
EcoT38I	GRGCYC	4 cut(s)	230, 312, 463, 481
ErhI	CCWWGG	5 cut(s)	285, 343, 1012, 1203, 2334
Esp3I	CGTCTC	2 cut(s)	700, 1211
FalI	AAGNNNNNCTT	1 cut(s)	2558
FaqI	GGGAC	4 cut(s)	3, 35, 70, 537
FauI	CCCGC	2 cut(s)	468, 503
FbaI	TGATCA	2 cut(s)	873, 2254
FblI	GTMKAC	2 cut(s)	65, 756
Fnu4HI	GCNGC	9 cut(s)	133, 369, 453, 473, 867, 1051, 1212, 1347, 1350
FokI	GGATG	4 cut(s)	581, 1549, 1731, 1781
FriOI	GRGCYC	4 cut(s)	230, 312, 463, 481
Fsp4HI	GCNGC	9 cut(s)	133, 369, 453, 473, 867, 1051, 1212, 1347, 1350
FspBI	CTAG	6 cut(s)	33, 98, 485, 2196, 2292, 2388
GlaI	GCGC	4 cut(s)	359, 507, 696, 2325
GluI	GCNGC	9 cut(s)	133, 369, 453, 473, 867, 1051, 1212, 1347, 1350
GsaI	CCCAGC	4 cut(s)	701, 1383, 1724, 1869
HaeII	RGCGCY	1 cut(s)	2327
HgaI	GACGC	5 cut(s)	38, 124, 183, 342, 1228
HhaI	GCGC	4 cut(s)	360, 508, 697, 2326
Hin1I	GRCGYC	1 cut(s)	194
Hin6I	GCGC	4 cut(s)	358, 506, 695, 2324
HinP1I	GCGC	4 cut(s)	358, 506, 695, 2324
HincII	GTYRAC	2 cut(s)	757, 2371
HindII	GTYRAC	2 cut(s)	757, 2371
HindIII	AAGCTT	2 cut(s)	1919, 2516
HphI	GGTGA	9 cut(s)	103, 163, 772, 1189, 1540, 1583, 1784, 1820, 2323
Hpy166II	GTNNAC	5 cut(s)	66, 757, 1697, 1792, 2371
Hpy8I	GTNNAC	5 cut(s)	66, 757, 1697, 1792, 2371
Hpy99I	CGWCG	5 cut(s)	89, 140, 151, 217, 358
HpyAV	CCTTC	7 cut(s)	505, 661, 838, 1017, 1499, 1618, 2300
HpyCH4III	ACNGT	5 cut(s)	151, 687, 1190, 1612, 1701
HpyCH4IV	ACGT	5 cut(s)	48, 953, 1136, 1478, 1482
HpySE526I	ACGT	5 cut(s)	48, 953, 1136, 1478, 1482
Hsp92I	GRCGYC	1 cut(s)	194
HspAI	GCGC	4 cut(s)	358, 506, 695, 2324
Kpn2I	TCCGGA	1 cut(s)	500
KroI	GCCGGC	1 cut(s)	142
KroNI	GCCGGC	1 cut(s)	144
Ksp22I	TGATCA	2 cut(s)	873, 2254
Kzo9I	GATC	9 cut(s)	487, 801, 873, 889, 1035, 1122, 2248, 2254, 2318
Lsp1109I	GCAGC	6 cut(s)	144, 484, 878, 1062, 1333, 1361
LweI	GCATC	5 cut(s)	559, 682, 1753, 2265, 2302
MaeI	CTAG	6 cut(s)	33, 98, 485, 2196, 2292, 2388
MaeII	ACGT	5 cut(s)	48, 953, 1136, 1478, 1482
MaeIII	GTNAC	6 cut(s)	91, 1248, 1510, 1528, 1808, 2191
MalI	GATC	9 cut(s)	489, 803, 875, 891, 1037, 1124, 2250, 2256, 2320
MbiI	CCGCTC	2 cut(s)	498, 630
MboI	GATC	9 cut(s)	487, 801, 873, 889, 1035, 1122, 2248, 2254, 2318
MfeI	CAATTG	1 cut(s)	923
MflI	RGATCY	2 cut(s)	889, 2248
MhlI	GDGCHC	8 cut(s)	230, 312, 463, 481, 1721, 1948, 2491, 2574
MlsI	TGGCCA	2 cut(s)	284, 2501
MluCI	AATT	8 cut(s)	592, 923, 1518, 1755, 2095, 2438, 2552, 2558
MluNI	TGGCCA	2 cut(s)	284, 2501
MlyI	GAGTC	7 cut(s)	6, 71, 88, 434, 708, 1083, 1596
MmeI	TCCRAC	2 cut(s)	258, 867
Mox20I	TGGCCA	2 cut(s)	284, 2501
Mph1103I	ATGCAT	2 cut(s)	574, 650
MroI	TCCGGA	1 cut(s)	500
MroNI	GCCGGC	1 cut(s)	142
MroXI	GAANNNNTTC	1 cut(s)	2429
MscI	TGGCCA	2 cut(s)	284, 2501
MseI	TTAA	6 cut(s)	156, 998, 1494, 1683, 1827, 1925
MslI	CAYNNNNRTG	5 cut(s)	348, 653, 2273, 2333, 2457
Msp20I	TGGCCA	2 cut(s)	284, 2501
MspA1I	CMGCKG	2 cut(s)	475, 1724
MspCI	CTTAAG	2 cut(s)	997, 1826
MspR9I	CCNGG	8 cut(s)	54, 55, 424, 521, 1291, 1641, 1770, 1949
MunI	CAATTG	1 cut(s)	923
MvaI	CCWGG	1 cut(s)	1291
MvnI	CGCG	2 cut(s)	358, 360
NaeI	GCCGGC	1 cut(s)	144
NciI	CCSGG	7 cut(s)	54, 55, 424, 521, 1641, 1770, 1949
NcoI	CCATGG	2 cut(s)	343, 2334
NdeII	GATC	9 cut(s)	487, 801, 873, 889, 1035, 1122, 2248, 2254, 2318
NgoMIV	GCCGGC	1 cut(s)	142
NlaIV	GGNNCC	8 cut(s)	178, 341, 421, 494, 891, 1385, 1501, 1945
NmeAIII	GCCGAG	2 cut(s)	205, 422
NmuCI	GTSAC	4 cut(s)	91, 1510, 1528, 1808
NsiI	ATGCAT	2 cut(s)	574, 650
NspI	RCATGY	1 cut(s)	1708
NspV	TTCGAA	1 cut(s)	665
OliI	CACNNNNGTG	1 cut(s)	2457
PaeR7I	CTCGAG	1 cut(s)	202
PceI	AGGCCT	2 cut(s)	1017, 2413
PciI	ACATGT	1 cut(s)	1704
PcsI	WCGNNNNNNNCGW	2 cut(s)	209, 747
PdiI	GCCGGC	1 cut(s)	144
PdmI	GAANNNNTTC	1 cut(s)	2429
PfeI	GAWTC	8 cut(s)	243, 662, 777, 1044, 1427, 1578, 2072, 2123
Pfl23II	CGTACG	1 cut(s)	1479
PflFI	GACNNNGTC	2 cut(s)	15, 61
PflMI	CCANNNNNTGG	1 cut(s)	1275
PfoI	TCCNGGA	1 cut(s)	1639
PinAI	ACCGGT	1 cut(s)	1252
PkrI	GCNGC	9 cut(s)	134, 370, 454, 474, 868, 1052, 1213, 1348, 1351
PleI	GAGTC	7 cut(s)	6, 70, 88, 434, 708, 1083, 1595
PpsI	GAGTC	7 cut(s)	6, 70, 88, 434, 708, 1083, 1595
Ppu21I	YACGTR	4 cut(s)	954, 1137, 1479, 1483
PscI	ACATGT	1 cut(s)	1704
PshAI	GACNNNNGTC	1 cut(s)	33
PshBI	ATTAAT	1 cut(s)	156
PsiI	TTATAA	2 cut(s)	1415, 1523
Psp124BI	GAGCTC	2 cut(s)	230, 312
Psp6I	CCWGG	1 cut(s)	1289
PspFI	CCCAGC	4 cut(s)	697, 1379, 1720, 1865
PspGI	CCWGG	1 cut(s)	1289
PspLI	CGTACG	1 cut(s)	1479
PspN4I	GGNNCC	8 cut(s)	178, 341, 421, 494, 891, 1385, 1501, 1945
PspPI	GGNCC	7 cut(s)	4, 492, 514, 700, 1500, 1934, 2338
PspXI	VCTCGAGB	1 cut(s)	202
PstI	CTGCAG	1 cut(s)	1351
PstNI	CAGNNNCTG	1 cut(s)	543
PsuI	RGATCY	2 cut(s)	889, 2248
PsyI	GACNNNGTC	2 cut(s)	15, 61
PvuII	CAGCTG	1 cut(s)	1724
RsaI	GTAC	6 cut(s)	956, 1154, 1187, 1481, 1703, 2119
RsaNI	GTAC	6 cut(s)	955, 1153, 1186, 1480, 1702, 2118
RseI	CAYNNNNRTG	5 cut(s)	348, 653, 2273, 2333, 2457
SacI	GAGCTC	2 cut(s)	230, 312
SalI	GTCGAC	1 cut(s)	755
SaqAI	TTAA	6 cut(s)	156, 998, 1494, 1683, 1827, 1925
SatI	GCNGC	9 cut(s)	133, 369, 453, 473, 867, 1051, 1212, 1347, 1350
Sau3AI	GATC	9 cut(s)	487, 801, 873, 889, 1035, 1122, 2248, 2254, 2318
Sau96I	GGNCC	7 cut(s)	4, 492, 514, 700, 1500, 1934, 2338
SchI	GAGTC	7 cut(s)	6, 71, 88, 434, 708, 1083, 1596
ScrFI	CCNGG	8 cut(s)	54, 55, 424, 521, 1291, 1641, 1770, 1949
SduI	GDGCHC	8 cut(s)	230, 312, 463, 481, 1721, 1948, 2491, 2574
SfaNI	GCATC	5 cut(s)	559, 682, 1753, 2265, 2302
SfcI	CTRYAG	2 cut(s)	964, 1347
Sfr274I	CTCGAG	1 cut(s)	202
SfuI	TTCGAA	1 cut(s)	665
SinI	GGWCC	3 cut(s)	492, 1934, 2338
SlaI	CTCGAG	1 cut(s)	202
SmaI	CCCGGG	1 cut(s)	55
SmiMI	CAYNNNNRTG	5 cut(s)	348, 653, 2273, 2333, 2457
SmlI	CTYRAG	6 cut(s)	202, 258, 311, 716, 997, 1826
SmoI	CTYRAG	6 cut(s)	202, 258, 311, 716, 997, 1826
SnaBI	TACGTA	1 cut(s)	1483
SpeI	ACTAGT	1 cut(s)	2291
Sse9I	AATT	8 cut(s)	592, 923, 1518, 1755, 2095, 2438, 2552, 2558
SseBI	AGGCCT	2 cut(s)	1017, 2413
SspI	AATATT	2 cut(s)	160, 2047
SspMI	CTAG	6 cut(s)	33, 98, 485, 2196, 2292, 2388
SstI	GAGCTC	2 cut(s)	230, 312
StuI	AGGCCT	2 cut(s)	1017, 2413
StyD4I	CCNGG	8 cut(s)	52, 53, 422, 519, 1289, 1639, 1768, 1947
StyI	CCWWGG	5 cut(s)	285, 343, 1012, 1203, 2334
TaaI	ACNGT	5 cut(s)	151, 687, 1190, 1612, 1701
TaiI	ACGT	5 cut(s)	51, 956, 1139, 1481, 1485
TaqI	TCGA	9 cut(s)	87, 138, 203, 215, 665, 690, 756, 1314, 2317
TasI	AATT	8 cut(s)	592, 923, 1518, 1755, 2095, 2438, 2552, 2558
TatI	WGTACW	3 cut(s)	1152, 1701, 2117
TauI	GCSGC	3 cut(s)	371, 455, 1214
TfiI	GAWTC	8 cut(s)	243, 662, 777, 1044, 1427, 1578, 2072, 2123
Tru1I	TTAA	6 cut(s)	156, 998, 1494, 1683, 1827, 1925
Tru9I	TTAA	6 cut(s)	156, 998, 1494, 1683, 1827, 1925
TscAI	CASTG	4 cut(s)	47, 981, 1721, 2166
TseFI	GTSAC	4 cut(s)	91, 1510, 1528, 1808
TseI	GCWGC	6 cut(s)	132, 472, 866, 1050, 1346, 1349
Tsp45I	GTSAC	4 cut(s)	91, 1510, 1528, 1808
TspDTI	ATGAA	7 cut(s)	523, 671, 780, 1163, 1908, 2005, 2249
TspGWI	ACGGA	4 cut(s)	1537, 1604, 1737, 1787
TspMI	CCCGGG	1 cut(s)	53
TspRI	CASTG	4 cut(s)	47, 981, 1721, 2166
Tth111I	GACNNNGTC	2 cut(s)	15, 61
Van91I	CCANNNNNTGG	1 cut(s)	1275
Vha464I	CTTAAG	2 cut(s)	997, 1826
VpaK11BI	GGWCC	3 cut(s)	492, 1934, 2338
VspI	ATTAAT	1 cut(s)	156
XapI	RAATTY	4 cut(s)	2095, 2438, 2552, 2558
XbaI	TCTAGA	1 cut(s)	97
XceI	RCATGY	1 cut(s)	1708
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2360
XhoI	CTCGAG	1 cut(s)	202
XmaI	CCCGGG	1 cut(s)	53
XmiI	GTMKAC	2 cut(s)	65, 756
XmnI	GAANNNNTTC	1 cut(s)	2429
XspI	CTAG	6 cut(s)	33, 98, 485, 2196, 2292, 2388
Zsp2I	ATGCAT	2 cut(s)	574, 650

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.