Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0014884

Physical Location & Seq

Genomic Coordinates Reverse (-)

22959160 .. 22964657

Gene Structure 5498 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1095900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGGGCAACAACTGCAGCAGCGGCACTCTCCCCTCCTCCACCCAAGACCCGGCCGACCACCACCACCACCCATCAAACGGTGCCGTCCGCATCCTCCCTCCCAATGCCCCCGCCCCACCGAAACCCAAACATCACACACCCCCAACCACCACCTCCGCTTCCAGCACCGCTCCCATCGGCCGGGTCCTCGGCCGGCCCATGGAAGACGTCCGGTCCACCTACACCTTCGGCCGCGAGCTCGGCCGCGGCCAATTCGGCGTCACCTACCTCGTCACTGATAAGCAGACCAAGCAGCTGTTTGCCTGCAAATCCATCGCCACCCGCAAGCTCACCATTCAAGACGACGTGGAGGACGTCCGCCGCGAGGTCCAGATCATGCACCACCTCACCGGCCACCGCAACATCGTCGAGCTCAAGGGAGCTTACGAGGACCGACACTCCGTGAACCTCGTCATGGAGCTCTGCGCCGGCGGCGAGCTCTTCGATCGGATCATTGCCAAAGGGCATTACTCCGAGCGAGCTGCGGCCAATCTCTGCCGCCAGATCGTCACCGTGGTCCACTACTGTCACTCGATGGGGGTTATGCATCGGGATTTGAAGCCGGAGAACTTCTTGCTCTTGAGCAAGGACGAGGACTCGCCGCTCAAGGCCACCGATTTCGGGCTTTCGGTGTTTTTTAAGCCAGGAGATGTATTTAAGGATCTTGTGGGAAGTGCATATTATGTTGCTCCTGAAGTGCTACGTCGTCGTTATGGAGCTGAGGCTGATATTTGGAGTGCAGGGGTGATACTATACATTCTACTTTGTGGGGTACCACCATTCTGGGGAGAGAATGAACAAGGTATCTTTGACGCAATCCTTCGGGGACATCTTGACTTCTCATCTGACCCTTGGCCTTCCATATCCAGCAGTGCCAAAGATCTTGTGAAGAAAATGCTACGAGCTGATCCTAAGGAACGACTTATAGCGGCTGATGTCCTAAGCCATCCATGGATGCGAGAAGATGGTGATGCATCTGATAAACCTCTTGATATTGCCGTTCTATCTAGAATGAAACAGTTCAGGGCAATGAACAAACTCAAGAAAGTAGCATTGAAGGTAATTGCAGAAAATCTTTCTGAAGAGGAAATCATAGGCTTGAAGGAAATATTCAAATCCATGGACACAGATAACAGTGGAACAATTACATATGAAGAGTTAAAAGCTGGCCTTCCAAAACTTGGTACCAAGCTCTCTGAGTCAGAAGTGAGACAGTTGATGGAAGCGGCTGATGTGGATGGAAATGGAACAATTGACTACATCGAGTTTATAACAGCTACTATGCACATGAATAGAATGGAAAGAGAGGACCATTTATACACAGCCTTTGAATATTTTGACAAAGACAAGAGCGGGTACATCACGATGGAAGAATTGGAGCAAGCACTTAAGAAGTATAATATGGGCGATGAGAAAACAATTAAGGAAATCATTGCAGAAGTTGACACGGACCGTGATGGAAGGATTAACTACGATGAATTTGCAGCCATGATGAGGAAAGGCAATCCTGAGTTGGTCACAAATAGACGAAGAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010119 GO:0010359 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

526

Amino Acids

59.02

Weight (kDa)

6.09

Isoelectric Point (pI)

38.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	74 - 332	2.9e-77	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	75 - 324	1.5e-28	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	380 - 406	2.9e-07	EF hand domain
EF-hand_7	PF13499	380 - 440	1.7e-13	EF-hand domain pair
EF-hand_6	PF13405	380 - 408	1e-06	EF-hand domain
EF-hand_8	PF13833	392 - 439	4e-06	EF-hand domain pair
EF-hand_7	PF13499	449 - 512	6e-17	EF-hand domain pair
EF-hand_6	PF13405	452 - 479	6.4e-07	EF-hand domain
EF-hand_1	PF00036	454 - 478	2.5e-06	EF hand domain
EF-hand_8	PF13833	464 - 513	7.9e-08	EF-hand domain pair
EF-hand_1	PF00036	487 - 513	4.5e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014884)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23650
fragaria_vesca	FvH4_5g03610 FvH4_5g03610 FvH4_5g03610
malus_domestica	MD06G1095900.v1.1 MD14G1116100.v1.1
prunus_persica	Prupe.5G110500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0196111
rosa_laevigata	RLG00000004064
rosa_multiflora	Rmu_sc0013391.1_g000002
rosa_roxburghii	Rroxscaffold_3G00259800
rosa_rugosa	Rorug07G0030700
rosa_samantha	Rh7AG156500 Rh7BG158100 Rh7CG162000
rosa_wichuraiana	Rw7G013370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1310
AatII	GACGTC	2 cut(s)	210, 357
Acc65I	GGTACC	2 cut(s)	811, 1223
AccB1I	GGYRCC	3 cut(s)	80, 811, 1223
AccB7I	CCANNNNNTGG	1 cut(s)	1220
AccBSI	CCGCTC	3 cut(s)	170, 643, 1392
AccII	CGCG	3 cut(s)	234, 246, 363
AclWI	GGATC	3 cut(s)	497, 708, 941
AcoI	YGGCCR	8 cut(s)	51, 178, 190, 229, 241, 247, 391, 525
AcsI	RAATTY	1 cut(s)	1517
AcuI	CTGAAG	2 cut(s)	753, 1140
AcyI	GRCGYC	3 cut(s)	207, 258, 354
AdeI	CACNNNGTG	1 cut(s)	442
AfaI	GTAC	3 cut(s)	813, 1225, 1397
AflII	CTTAAG	1 cut(s)	1427
AgsI	TTSAA	6 cut(s)	338, 598, 1096, 1141, 1153, 1370
AhdI	GACNNNNNGTC	1 cut(s)	211
AjiI	CACGTC	1 cut(s)	346
AjnI	CCWGG	1 cut(s)	682
Alw21I	GWGCWC	4 cut(s)	240, 414, 462, 480
Alw26I	GTCTC	1 cut(s)	1243
AlwI	GGATC	3 cut(s)	497, 708, 941
ApeKI	GCWGC	5 cut(s)	15, 18, 292, 521, 1523
ApoI	RAATTY	1 cut(s)	1517
Asp700I	GAANNNNTTC	1 cut(s)	1058
Asp718I	GGTACC	2 cut(s)	811, 1223
AspLEI	GCGC	1 cut(s)	467
AspS9I	GGNCC	8 cut(s)	184, 195, 213, 367, 430, 556, 1348, 1489
AsuC2I	CCSGG	2 cut(s)	50, 182
AsuHPI	GGTGA	6 cut(s)	253, 322, 379, 541, 796, 1019
AvaII	GGWCC	7 cut(s)	184, 213, 367, 430, 556, 1348, 1489
AxyI	CCTNAGG	1 cut(s)	951
BanI	GGYRCC	3 cut(s)	80, 811, 1223
BanII	GRGCYC	4 cut(s)	240, 414, 462, 480
BbsI	GAAGAC	1 cut(s)	210
Bbv12I	GWGCWC	4 cut(s)	240, 414, 462, 480
BbvCI	CCTCAGC	1 cut(s)	759
BbvI	GCAGC	5 cut(s)	27, 30, 304, 508, 1535
BceAI	ACGGC	2 cut(s)	68, 1022
BcgI	CGANNNNNNTGC	8 cut(s)	295, 329, 388, 422, 503, 537, 1502, 1536
BciT130I	CCWGG	1 cut(s)	684
BcnI	CCSGG	2 cut(s)	50, 182
BcoDI	GTCTC	1 cut(s)	1243
BfaI	CTAG	1 cut(s)	1047
BfmI	CTRYAG	1 cut(s)	13
BfrI	CTTAAG	1 cut(s)	1427
BglI	GCCNNNNNGGC	1 cut(s)	255
BglII	AGATCT	1 cut(s)	919
Bme1390I	CCNGG	3 cut(s)	50, 182, 684
Bme18I	GGWCC	7 cut(s)	184, 213, 367, 430, 556, 1348, 1489
BmeRI	GACNNNNNGTC	1 cut(s)	211
BmgBI	CACGTC	1 cut(s)	346
BmgT120I	GGNCC	8 cut(s)	184, 195, 213, 367, 430, 556, 1348, 1489
BmiI	GGNNCC	4 cut(s)	82, 185, 813, 1225
BmrFI	CCNGG	3 cut(s)	50, 182, 684
BmsI	GCATC	5 cut(s)	99, 595, 984, 1000, 1022
BpiI	GAAGAC	1 cut(s)	210
Bpu10I	CCTNAGC	2 cut(s)	759, 980
BpuEI	CTTGAG	4 cut(s)	398, 629, 640, 1064
BpuMI	CCSGG	2 cut(s)	50, 182
BsaHI	GRCGYC	3 cut(s)	207, 258, 354
BsaJI	CCNNGG	7 cut(s)	187, 198, 244, 552, 890, 989, 1158
BsaWI	WCCGGW	1 cut(s)	210
BsaXI	ACNNNNNCTCC	2 cut(s)	422, 452
Bse118I	RCCGGY	3 cut(s)	192, 389, 467
Bse21I	CCTNAGG	1 cut(s)	951
Bse3DI	GCAATG	3 cut(s)	492, 1074, 1470
BseBI	CCWGG	1 cut(s)	684
BseDI	CCNNGG	7 cut(s)	187, 198, 244, 552, 890, 989, 1158
BseGI	GGATG	4 cut(s)	90, 985, 999, 1282
BseMI	GCAATG	3 cut(s)	492, 1074, 1470
BseMII	CTCAG	3 cut(s)	750, 1227, 1539
BseRI	GAGGAG	1 cut(s)	25
BseX3I	CGGCCG	5 cut(s)	51, 178, 190, 229, 241
BseXI	GCAGC	5 cut(s)	27, 30, 304, 508, 1535
BsgI	GTGCAG	1 cut(s)	798
Bsh1236I	CGCG	3 cut(s)	234, 246, 363
Bsh1285I	CGRYCG	6 cut(s)	54, 181, 193, 232, 244, 487
BshNI	GGYRCC	3 cut(s)	80, 811, 1223
BsiEI	CGRYCG	6 cut(s)	54, 181, 193, 232, 244, 487
BsiHKAI	GWGCWC	4 cut(s)	240, 414, 462, 480
BsiSI	CCGG	7 cut(s)	50, 181, 193, 211, 390, 468, 602
BslFI	GGGAC	1 cut(s)	879
BsmAI	GTCTC	1 cut(s)	1243
BsmFI	GGGAC	1 cut(s)	879
Bsp1286I	GDGCHC	4 cut(s)	240, 414, 462, 480
Bsp143I	GATC	7 cut(s)	372, 484, 489, 543, 700, 919, 946
Bsp19I	CCATGG	3 cut(s)	198, 989, 1158
BspCNI	CTCAG	3 cut(s)	751, 1228, 1540
BspFNI	CGCG	3 cut(s)	234, 246, 363
BspLI	GGNNCC	4 cut(s)	82, 185, 813, 1225
BspMAI	CTGCAG	1 cut(s)	17
BspPI	GGATC	3 cut(s)	497, 708, 941
BspQI	GCTCTTC	1 cut(s)	485
BspT107I	GGYRCC	3 cut(s)	80, 811, 1223
BspTI	CTTAAG	1 cut(s)	1427
BsrBI	CCGCTC	3 cut(s)	170, 643, 1392
BsrDI	GCAATG	3 cut(s)	492, 1074, 1470
BsrFI	RCCGGY	3 cut(s)	192, 389, 467
BssAI	RCCGGY	3 cut(s)	192, 389, 467
BssECI	CCNNGG	7 cut(s)	187, 198, 244, 552, 890, 989, 1158
BssMI	GATC	7 cut(s)	372, 484, 489, 543, 700, 919, 946
BssNI	GRCGYC	3 cut(s)	207, 258, 354
BssT1I	CCWWGG	4 cut(s)	198, 890, 989, 1158
Bst2UI	CCWGG	1 cut(s)	684
Bst4CI	ACNGT	7 cut(s)	80, 553, 566, 1059, 1175, 1254, 1493
Bst6I	CTCTTC	3 cut(s)	485, 1116, 1188
BstACI	GRCGYC	3 cut(s)	207, 258, 354
BstAFI	CTTAAG	1 cut(s)	1427
BstAPI	GCANNNNNTGC	1 cut(s)	12
BstC8I	GCNNGC	9 cut(s)	194, 236, 304, 326, 469, 476, 519, 1207, 1422
BstDEI	CTNAG	5 cut(s)	759, 951, 980, 1236, 1548
BstDSI	CCRYGG	5 cut(s)	198, 244, 552, 989, 1158
BstF5I	GGATG	4 cut(s)	90, 985, 999, 1282
BstFNI	CGCG	3 cut(s)	234, 246, 363
BstHHI	GCGC	1 cut(s)	467
BstKTI	GATC	7 cut(s)	375, 487, 492, 546, 703, 922, 949
BstMAI	GTCTC	1 cut(s)	1243
BstMBI	GATC	7 cut(s)	372, 484, 489, 543, 700, 919, 946
BstMCI	CGRYCG	6 cut(s)	54, 181, 193, 232, 244, 487
BstMWI	GCNNNNNNNGC	6 cut(s)	12, 21, 240, 255, 289, 471
BstNI	CCWGG	1 cut(s)	684
BstSCI	CCNGG	3 cut(s)	48, 180, 682
BstSFI	CTRYAG	1 cut(s)	13
BstUI	CGCG	3 cut(s)	234, 246, 363
BstV1I	GCAGC	5 cut(s)	27, 30, 304, 508, 1535
BstV2I	GAAGAC	1 cut(s)	210
BstX2I	RGATCY	2 cut(s)	700, 919
BstXI	CCANNNNNNTGG	1 cut(s)	822
BstYI	RGATCY	2 cut(s)	700, 919
BstZI	CGGCCG	5 cut(s)	51, 178, 190, 229, 241
Bsu36I	CCTNAGG	1 cut(s)	951
BtgI	CCRYGG	5 cut(s)	198, 244, 552, 989, 1158
BtgZI	GCGATG	2 cut(s)	298, 1461
BtrI	CACGTC	1 cut(s)	346
BtsCI	GGATG	4 cut(s)	90, 985, 999, 1282
BtsI	GCAGTG	1 cut(s)	916
BtsIMutI	CAGTG	3 cut(s)	273, 916, 1180
Cac8I	GCNNGC	9 cut(s)	194, 236, 304, 326, 469, 476, 519, 1207, 1422
CfoI	GCGC	1 cut(s)	467
Cfr10I	RCCGGY	3 cut(s)	192, 389, 467
Cfr13I	GGNCC	8 cut(s)	184, 195, 213, 367, 430, 556, 1348, 1489
Cfr42I	CCGCGG	1 cut(s)	247
CpoI	CGGWCCG	1 cut(s)	1489
CseI	GACGC	2 cut(s)	247, 860
Csp6I	GTAC	3 cut(s)	812, 1224, 1396
CspI	CGGWCCG	1 cut(s)	1489
CviAII	CATG	7 cut(s)	199, 376, 454, 990, 1159, 1327, 1528
CviQI	GTAC	3 cut(s)	812, 1224, 1396
DdeI	CTNAG	5 cut(s)	759, 951, 980, 1236, 1548
DpnI	GATC	7 cut(s)	374, 486, 491, 545, 702, 921, 948
DpnII	GATC	7 cut(s)	372, 484, 489, 543, 700, 919, 946
DraIII	CACNNNGTG	1 cut(s)	442
DriI	GACNNNNNGTC	1 cut(s)	211
EaeI	YGGCCR	8 cut(s)	51, 178, 190, 229, 241, 247, 391, 525
EagI	CGGCCG	5 cut(s)	51, 178, 190, 229, 241
Eam1104I	CTCTTC	3 cut(s)	485, 1116, 1188
Eam1105I	GACNNNNNGTC	1 cut(s)	211
EarI	CTCTTC	3 cut(s)	485, 1116, 1188
EciI	GGCGGA	1 cut(s)	347
Ecl136II	GAGCTC	4 cut(s)	238, 412, 460, 478
EclXI	CGGCCG	5 cut(s)	51, 178, 190, 229, 241
Eco130I	CCWWGG	4 cut(s)	198, 890, 989, 1158
Eco24I	GRGCYC	4 cut(s)	240, 414, 462, 480
Eco47I	GGWCC	7 cut(s)	184, 213, 367, 430, 556, 1348, 1489
Eco52I	CGGCCG	5 cut(s)	51, 178, 190, 229, 241
Eco53kI	GAGCTC	4 cut(s)	238, 412, 460, 478
Eco57I	CTGAAG	2 cut(s)	753, 1140
Eco81I	CCTNAGG	1 cut(s)	951
EcoICRI	GAGCTC	4 cut(s)	238, 412, 460, 478
EcoO109I	RGGNCCY	1 cut(s)	184
EcoRII	CCWGG	1 cut(s)	682
EcoT14I	CCWWGG	4 cut(s)	198, 890, 989, 1158
EcoT22I	ATGCAT	2 cut(s)	588, 1015
EcoT38I	GRGCYC	4 cut(s)	240, 414, 462, 480
ErhI	CCWWGG	4 cut(s)	198, 890, 989, 1158
FaeI	CATG	7 cut(s)	202, 379, 457, 993, 1162, 1330, 1531
FalI	AAGNNNNNCTT	2 cut(s)	1194, 1226
FaqI	GGGAC	1 cut(s)	879
FatI	CATG	7 cut(s)	198, 375, 453, 989, 1158, 1326, 1527
FauI	CCCGC	3 cut(s)	118, 329, 1385
FauNDI	CATATG	1 cut(s)	1189
FokI	GGATG	4 cut(s)	77, 972, 1006, 1289
FriOI	GRGCYC	4 cut(s)	240, 414, 462, 480
FseI	GGCCGGCC	1 cut(s)	196
FspBI	CTAG	1 cut(s)	1047
GlaI	GCGC	1 cut(s)	466
HapII	CCGG	7 cut(s)	50, 181, 193, 211, 390, 468, 602
HgaI	GACGC	2 cut(s)	247, 860
HhaI	GCGC	1 cut(s)	467
Hin1I	GRCGYC	3 cut(s)	207, 258, 354
Hin1II	CATG	7 cut(s)	202, 379, 457, 993, 1162, 1330, 1531
Hin6I	GCGC	1 cut(s)	465
HinP1I	GCGC	1 cut(s)	465
HincII	GTYRAC	1 cut(s)	1483
HindII	GTYRAC	1 cut(s)	1483
HinfI	GANTC	2 cut(s)	635, 1238
HpaII	CCGG	7 cut(s)	50, 181, 193, 211, 390, 468, 602
HphI	GGTGA	6 cut(s)	253, 322, 379, 541, 796, 1019
Hpy166II	GTNNAC	4 cut(s)	216, 445, 559, 1483
Hpy188I	TCNGA	7 cut(s)	489, 514, 886, 1018, 1120, 1237, 1243
Hpy8I	GTNNAC	4 cut(s)	216, 445, 559, 1483
Hpy99I	CGWCG	4 cut(s)	347, 410, 747, 750
HpyAV	CCTTC	7 cut(s)	235, 869, 906, 1090, 1135, 1220, 1494
HpyCH4III	ACNGT	7 cut(s)	80, 553, 566, 1059, 1175, 1254, 1493
HpyCH4IV	ACGT	4 cut(s)	207, 345, 354, 742
HpyF10VI	GCNNNNNNNGC	6 cut(s)	12, 21, 240, 255, 289, 471
HpyF3I	CTNAG	5 cut(s)	759, 951, 980, 1236, 1548
HpySE526I	ACGT	4 cut(s)	207, 345, 354, 742
Hsp92I	GRCGYC	3 cut(s)	207, 258, 354
Hsp92II	CATG	7 cut(s)	202, 379, 457, 993, 1162, 1330, 1531
HspAI	GCGC	1 cut(s)	465
KpnI	GGTACC	2 cut(s)	815, 1227
KroI	GCCGGC	2 cut(s)	192, 467
KroNI	GCCGGC	2 cut(s)	194, 469
KspI	CCGCGG	1 cut(s)	247
Kzo9I	GATC	7 cut(s)	372, 484, 489, 543, 700, 919, 946
LguI	GCTCTTC	1 cut(s)	485
LmnI	GCTCC	6 cut(s)	175, 419, 457, 733, 755, 1417
Lsp1109I	GCAGC	5 cut(s)	27, 30, 304, 508, 1535
LweI	GCATC	5 cut(s)	99, 595, 984, 1000, 1022
MaeI	CTAG	1 cut(s)	1047
MaeII	ACGT	4 cut(s)	207, 345, 354, 742
MaeIII	GTNAC	5 cut(s)	259, 271, 547, 566, 1555
MalI	GATC	7 cut(s)	374, 486, 491, 545, 702, 921, 948
MbiI	CCGCTC	3 cut(s)	170, 643, 1392
MboI	GATC	7 cut(s)	372, 484, 489, 543, 700, 919, 946
MboII	GAAGA	7 cut(s)	215, 472, 940, 1013, 1133, 1205, 1421
MfeI	CAATTG	1 cut(s)	1290
MflI	RGATCY	2 cut(s)	700, 919
MhlI	GDGCHC	4 cut(s)	240, 414, 462, 480
MluCI	AATT	7 cut(s)	251, 1101, 1182, 1290, 1412, 1458, 1517
MlyI	GAGTC	2 cut(s)	629, 1247
Mph1103I	ATGCAT	2 cut(s)	588, 1015
MreI	CGCCGGCG	1 cut(s)	467
MroNI	GCCGGC	2 cut(s)	192, 467
MroXI	GAANNNNTTC	1 cut(s)	1058
MseI	TTAA	6 cut(s)	678, 696, 1199, 1428, 1461, 1506
MslI	CAYNNNNRTG	1 cut(s)	1403
MspA1I	CMGCKG	3 cut(s)	21, 246, 295
MspCI	CTTAAG	1 cut(s)	1427
MspI	CCGG	7 cut(s)	50, 181, 193, 211, 390, 468, 602
MspR9I	CCNGG	3 cut(s)	50, 182, 684
MunI	CAATTG	1 cut(s)	1290
MvaI	CCWGG	1 cut(s)	684
MvnI	CGCG	3 cut(s)	234, 246, 363
MwoI	GCNNNNNNNGC	6 cut(s)	12, 21, 240, 255, 289, 471
NaeI	GCCGGC	2 cut(s)	194, 469
NciI	CCSGG	2 cut(s)	50, 182
NcoI	CCATGG	3 cut(s)	198, 989, 1158
NdeI	CATATG	1 cut(s)	1189
NdeII	GATC	7 cut(s)	372, 484, 489, 543, 700, 919, 946
NgoMIV	GCCGGC	2 cut(s)	192, 467
NlaIII	CATG	7 cut(s)	202, 379, 457, 993, 1162, 1330, 1531
NlaIV	GGNNCC	4 cut(s)	82, 185, 813, 1225
NmeAIII	GCCGAG	2 cut(s)	168, 219
NmuCI	GTSAC	5 cut(s)	259, 271, 547, 566, 1555
NsiI	ATGCAT	2 cut(s)	588, 1015
PciSI	GCTCTTC	1 cut(s)	485
PcsI	WCGNNNNNNNCGW	2 cut(s)	351, 360
PdiI	GCCGGC	2 cut(s)	194, 469
PdmI	GAANNNNTTC	1 cut(s)	1058
PflMI	CCANNNNNTGG	1 cut(s)	1220
Ple19I	CGATCG	1 cut(s)	487
PleI	GAGTC	2 cut(s)	629, 1246
PpsI	GAGTC	2 cut(s)	629, 1246
PpuMI	RGGWCCY	1 cut(s)	184
PsiI	TTATAA	1 cut(s)	1310
Psp124BI	GAGCTC	4 cut(s)	240, 414, 462, 480
Psp5II	RGGWCCY	1 cut(s)	184
Psp6I	CCWGG	1 cut(s)	682
PspGI	CCWGG	1 cut(s)	682
PspN4I	GGNNCC	4 cut(s)	82, 185, 813, 1225
PspPI	GGNCC	8 cut(s)	184, 195, 213, 367, 430, 556, 1348, 1489
PspPPI	RGGWCCY	1 cut(s)	184
PstI	CTGCAG	1 cut(s)	17
PsuI	RGATCY	2 cut(s)	700, 919
PvuI	CGATCG	1 cut(s)	487
PvuII	CAGCTG	1 cut(s)	295
RigI	GGCCGGCC	1 cut(s)	196
RsaI	GTAC	3 cut(s)	813, 1225, 1397
RsaNI	GTAC	3 cut(s)	812, 1224, 1396
RseI	CAYNNNNRTG	1 cut(s)	1403
Rsr2I	CGGWCCG	1 cut(s)	1489
RsrII	CGGWCCG	1 cut(s)	1489
SacI	GAGCTC	4 cut(s)	240, 414, 462, 480
SacII	CCGCGG	1 cut(s)	247
SapI	GCTCTTC	1 cut(s)	485
SaqAI	TTAA	6 cut(s)	678, 696, 1199, 1428, 1461, 1506
Sau3AI	GATC	7 cut(s)	372, 484, 489, 543, 700, 919, 946
Sau96I	GGNCC	8 cut(s)	184, 195, 213, 367, 430, 556, 1348, 1489
SchI	GAGTC	2 cut(s)	629, 1247
ScrFI	CCNGG	3 cut(s)	50, 182, 684
SduI	GDGCHC	4 cut(s)	240, 414, 462, 480
SfaNI	GCATC	5 cut(s)	99, 595, 984, 1000, 1022
SfcI	CTRYAG	1 cut(s)	13
Sfr303I	CCGCGG	1 cut(s)	247
SgrAI	CRCCGGYG	1 cut(s)	467
SgrBI	CCGCGG	1 cut(s)	247
SinI	GGWCC	7 cut(s)	184, 213, 367, 430, 556, 1348, 1489
SmiMI	CAYNNNNRTG	1 cut(s)	1403
SmlI	CTYRAG	5 cut(s)	413, 619, 644, 1079, 1427
SmoI	CTYRAG	5 cut(s)	413, 619, 644, 1079, 1427
Sse9I	AATT	7 cut(s)	251, 1101, 1182, 1290, 1412, 1458, 1517
SspI	AATATT	2 cut(s)	1149, 1373
SspMI	CTAG	1 cut(s)	1047
SstI	GAGCTC	4 cut(s)	240, 414, 462, 480
StyD4I	CCNGG	3 cut(s)	48, 180, 682
StyI	CCWWGG	4 cut(s)	198, 890, 989, 1158
TaaI	ACNGT	7 cut(s)	80, 553, 566, 1059, 1175, 1254, 1493
TaiI	ACGT	4 cut(s)	210, 348, 357, 745
TaqI	TCGA	4 cut(s)	408, 483, 572, 1302
TaqII	GACCGA	1 cut(s)	447
TasI	AATT	7 cut(s)	251, 1101, 1182, 1290, 1412, 1458, 1517
Tru1I	TTAA	6 cut(s)	678, 696, 1199, 1428, 1461, 1506
Tru9I	TTAA	6 cut(s)	678, 696, 1199, 1428, 1461, 1506
TscAI	CASTG	3 cut(s)	280, 916, 1180
TseFI	GTSAC	5 cut(s)	259, 271, 547, 566, 1555
TseI	GCWGC	5 cut(s)	15, 18, 292, 521, 1523
Tsp45I	GTSAC	5 cut(s)	259, 271, 547, 566, 1555
TspDTI	ATGAA	6 cut(s)	849, 1067, 1085, 1206, 1343, 1530
TspGWI	ACGGA	2 cut(s)	430, 1502
TspRI	CASTG	3 cut(s)	280, 916, 1180
Van91I	CCANNNNNTGG	1 cut(s)	1220
Vha464I	CTTAAG	1 cut(s)	1427
VpaK11BI	GGWCC	7 cut(s)	184, 213, 367, 430, 556, 1348, 1489
XapI	RAATTY	1 cut(s)	1517
XbaI	TCTAGA	1 cut(s)	1046
XmnI	GAANNNNTTC	1 cut(s)	1058
XspI	CTAG	1 cut(s)	1047
ZraI	GACGTC	2 cut(s)	208, 355
Zsp2I	ATGCAT	2 cut(s)	588, 1015

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.