Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0014884

Physical Location & Seq

Genomic Coordinates Reverse (-)

55785361 .. 55788973

Gene Structure 3613 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004064

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1554 bp

ATGGGCGGCAACTGCAGCACCGCCGCCGGCGACGCACCCGATCCCCGCCCCTCCAACGGCCACGTCCAAGTCCTCCCCCCAAATGCCGTCCCGACCCGGCCCAAACCCCACACCTCCGCCCCAACCACCACCACCACCAACGCCACCGCCATCGGCCGCGTCCTCGGCAAGCCCATGGAGGACGTCCGCTCCACCTACACCTTCGGCCGCGAGCTCGGCCGCGGCCAATTCGGCGTCACCTACCTCGTGACTCACAAAGAGACCAAGCAGCAGTTCGCCTGCAAATCCATCGCTACTCGGAAGCTGATCAACCACGACGACGTGGAGGACGTCCGCCGTGAGGTCCAGATCATGCACCACCTCACCGGCCACCGCAACATCGTCGAGCTCAAAGGCGCGTACGAGGACCGCCACTCGGTCAACCTGGTCATGGAGCTCTGCGCCGGCGGCGAGCTGTTCGATAGGATCATCGCCAAGGGGCACTATTCGGAGCGGGCGGCAGCGAATCTCTGCCGTCAGATGGTGACCGTCGTGCATTACTGTCACTCTATGGGGGTGTTTCACAGGGACTTGAAGCCGGAGAATTTCTTGCTGTTGAGTAAGGATGAGGACTCGCCGCTCAAGGCCACCGATTTCGGACTCTCCGTGTTCTTCAAGCCCGGAGATGTATTCAAGGATCTTGTGGGAAGTGCTTACTATGTTGCCCCCGAAGTGCTGCGCAGGCGTTATGGAGCTGAGGCTGATATTTGGAGTGCTGGAGTGATACTATACATTCTACTTAGTGGAGTCCCACCATTCTGGGGAGAGAATGAACAGGGTATCTTTGATGCTATTCTTCGGGGTCACATTGATTTTTCATCTGATCCTTGGCCTTCCATATCCAGCAGTGCCAAAGATCTTGTGAAGAAAATGCTTCGAGCTGATCCAAAGGAACGGCTTGCAGCAGTGGATGTCCTTAATCATCCATGGATGCGAGAAGATGGCGATGCATCTGATAAACCTCTTGATATTGCTGTTTTATCTAGAATGAAACAGTTCAGGGCAATGAACAAACTCAAGAAAGTTGCATTGAAGGTAATTGCAGAAAATCTTTCTGAAGAGGAAATCATAGGCTTGAAGGAAATGTTTAAATCAATGGACACAGATAACAGTGGAACAATTACATTTGAAGAGTTAAAAGCGGGCCTTCCAAAGCTGGGTACCAAGCTTTCTGAGTCTGAAGTGAGACAGTTGATGGAAGCGGCTGATGTTGATGGAAATGGAACAATTGATTACATTGAGTTTATAACCGCTACTATGCACATGAACAGAATGGATAGAGAGGACCATTTATACACAGCCTTTGAATATTTCGACAAGGACAAGAGCGGGTACATCACGATGGAAGAATTGGAGCAAGCACTTAAGAAGTATAATATGGGTGATGAACAAACGATTAAGGAAATCATCGCAGAAGTTGACACAGACCTTGATGGAAGGATCAACTATGATGAGTTTGTTGCCATGATGAGGAAAGGAAATCCCGAATTGGTCACAAACAGAAGAAGAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010119 GO:0010359 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

518

Amino Acids

58.11

Weight (kDa)

5.87

Isoelectric Point (pI)

35.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	66 - 324	1.6e-77	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	67 - 317	5e-29	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	372 - 398	7.4e-08	EF hand domain
EF-hand_7	PF13499	372 - 432	3.1e-13	EF-hand domain pair
EF-hand_6	PF13405	372 - 400	1.9e-06	EF-hand domain
EF-hand_8	PF13833	384 - 430	1.9e-07	EF-hand domain pair
EF-hand_7	PF13499	441 - 504	1.2e-16	EF-hand domain pair
EF-hand_6	PF13405	444 - 471	6.3e-07	EF-hand domain
EF-hand_1	PF00036	446 - 470	2.5e-06	EF hand domain
EF-hand_8	PF13833	456 - 505	5.5e-08	EF-hand domain pair
EF-hand_1	PF00036	479 - 505	3.5e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014884)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23650
fragaria_vesca	FvH4_5g03610 FvH4_5g03610 FvH4_5g03610
malus_domestica	MD06G1095900.v1.1 MD14G1116100.v1.1
prunus_persica	Prupe.5G110500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0196111
rosa_laevigata	RLG00000004064
rosa_multiflora	Rmu_sc0013391.1_g000002
rosa_roxburghii	Rroxscaffold_3G00259800
rosa_rugosa	Rorug07G0030700
rosa_samantha	Rh7AG156500 Rh7BG158100 Rh7CG162000
rosa_wichuraiana	Rw7G013370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1286
AatII	GACGTC	2 cut(s)	186, 333
Acc16I	TGCGCA	1 cut(s)	719
Acc65I	GGTACC	1 cut(s)	1199
AccB1I	GGYRCC	1 cut(s)	1199
AccBSI	CCGCTC	4 cut(s)	189, 493, 619, 1368
AccII	CGCG	4 cut(s)	159, 210, 222, 398
AclWI	GGATC	6 cut(s)	35, 473, 684, 857, 917, 1487
AcoI	YGGCCR	6 cut(s)	58, 154, 205, 217, 223, 367
AcsI	RAATTY	1 cut(s)	583
AcuI	CTGAAG	2 cut(s)	1116, 1239
AcyI	GRCGYC	3 cut(s)	183, 234, 330
AfaI	GTAC	3 cut(s)	401, 1201, 1373
AfiI	CCNNNNNNNGG	8 cut(s)	56, 340, 415, 430, 520, 800, 1196, 1509
AflII	CTTAAG	1 cut(s)	1403
AgsI	TTSAA	7 cut(s)	574, 655, 673, 1072, 1117, 1169, 1346
AjiI	CACGTC	2 cut(s)	64, 322
AjnI	CCWGG	1 cut(s)	423
AjuI	GAANNNNNNNTTGG	2 cut(s)	257, 289
AluBI	AGCT	9 cut(s)	214, 304, 388, 436, 454, 734, 920, 1195, 1207
AluI	AGCT	9 cut(s)	214, 304, 388, 436, 454, 734, 920, 1195, 1207
Alw21I	GWGCWC	3 cut(s)	216, 390, 438
Alw26I	GTCTC	2 cut(s)	254, 1219
AlwI	GGATC	6 cut(s)	35, 473, 684, 857, 917, 1487
ApeKI	GCWGC	5 cut(s)	15, 268, 500, 715, 941
ApoI	RAATTY	1 cut(s)	583
Asp700I	GAANNNNTTC	1 cut(s)	1034
Asp718I	GGTACC	1 cut(s)	1199
AspLEI	GCGC	3 cut(s)	398, 443, 720
AspS9I	GGNCC	5 cut(s)	99, 343, 406, 1183, 1324
AsuC2I	CCSGG	2 cut(s)	97, 660
AsuHPI	GGTGA	4 cut(s)	229, 355, 535, 1433
AvaII	GGWCC	3 cut(s)	343, 406, 1324
BaeGI	GKGCMC	1 cut(s)	483
BanI	GGYRCC	1 cut(s)	1199
BanII	GRGCYC	3 cut(s)	216, 390, 438
BauI	CACGAG	1 cut(s)	245
Bbv12I	GWGCWC	3 cut(s)	216, 390, 438
BbvCI	CCTCAGC	1 cut(s)	735
BbvI	GCAGC	5 cut(s)	27, 280, 512, 702, 953
BccI	CCATC	8 cut(s)	158, 296, 514, 974, 1228, 1247, 1375, 1466
BceAI	ACGGC	5 cut(s)	71, 73, 321, 498, 950
BcgI	CGANNNNNNTGC	4 cut(s)	271, 305, 364, 398
BciT130I	CCWGG	1 cut(s)	425
BclI	TGATCA	1 cut(s)	306
BcnI	CCSGG	2 cut(s)	97, 660
BcoDI	GTCTC	2 cut(s)	254, 1219
BfaI	CTAG	1 cut(s)	1023
BfmI	CTRYAG	1 cut(s)	13
BfrI	CTTAAG	1 cut(s)	1403
BglI	GCCNNNNNGGC	1 cut(s)	231
BglII	AGATCT	1 cut(s)	895
Bme1390I	CCNGG	3 cut(s)	97, 425, 660
Bme18I	GGWCC	3 cut(s)	343, 406, 1324
BmgBI	CACGTC	2 cut(s)	64, 322
BmgT120I	GGNCC	5 cut(s)	99, 343, 406, 1183, 1324
BmiI	GGNNCC	1 cut(s)	1201
BmrFI	CCNGG	3 cut(s)	97, 425, 660
BmsI	GCATC	4 cut(s)	817, 960, 976, 998
BpmI	CTGGAG	1 cut(s)	777
Bpu10I	CCTNAGC	1 cut(s)	735
BpuEI	CTTGAG	2 cut(s)	605, 1040
BpuMI	CCSGG	2 cut(s)	97, 660
BsaHI	GRCGYC	3 cut(s)	183, 234, 330
BsaI	GGTCTC	1 cut(s)	254
BsaJI	CCNNGG	6 cut(s)	163, 174, 220, 474, 866, 965
BsaXI	ACNNNNNCTCC	2 cut(s)	173, 203
Bsc4I	CCNNNNNNNGG	8 cut(s)	56, 340, 415, 430, 520, 800, 1196, 1509
Bse118I	RCCGGY	3 cut(s)	26, 365, 443
Bse3DI	GCAATG	1 cut(s)	1050
BseBI	CCWGG	1 cut(s)	425
BseDI	CCNNGG	6 cut(s)	163, 174, 220, 474, 866, 965
BseGI	GGATG	4 cut(s)	610, 955, 961, 975
BseLI	CCNNNNNNNGG	8 cut(s)	56, 340, 415, 430, 520, 800, 1196, 1509
BseMI	GCAATG	1 cut(s)	1050
BseMII	CTCAG	2 cut(s)	726, 1203
BseSI	GKGCMC	1 cut(s)	483
BseX3I	CGGCCG	3 cut(s)	154, 205, 217
BseXI	GCAGC	5 cut(s)	27, 280, 512, 702, 953
BseYI	CCCAGC	1 cut(s)	1195
Bsh1236I	CGCG	4 cut(s)	159, 210, 222, 398
Bsh1285I	CGRYCG	3 cut(s)	157, 208, 220
BshNI	GGYRCC	1 cut(s)	1199
BsiEI	CGRYCG	3 cut(s)	157, 208, 220
BsiHKAI	GWGCWC	3 cut(s)	216, 390, 438
BsiSI	CCGG	6 cut(s)	27, 97, 366, 444, 578, 660
BsiWI	CGTACG	1 cut(s)	399
BslFI	GGGAC	3 cut(s)	74, 581, 773
BslI	CCNNNNNNNGG	8 cut(s)	56, 340, 415, 430, 520, 800, 1196, 1509
BsmAI	GTCTC	2 cut(s)	254, 1219
BsmFI	GGGAC	3 cut(s)	74, 581, 773
Bso31I	GGTCTC	1 cut(s)	254
Bsp1286I	GDGCHC	4 cut(s)	216, 390, 438, 483
Bsp143I	GATC	9 cut(s)	40, 306, 348, 465, 676, 862, 895, 922, 1479
Bsp19I	CCATGG	2 cut(s)	174, 965
BspCNI	CTCAG	2 cut(s)	727, 1204
BspFNI	CGCG	4 cut(s)	159, 210, 222, 398
BspLI	GGNNCC	1 cut(s)	1201
BspMAI	CTGCAG	1 cut(s)	17
BspPI	GGATC	6 cut(s)	35, 473, 684, 857, 917, 1487
BspT107I	GGYRCC	1 cut(s)	1199
BspTI	CTTAAG	1 cut(s)	1403
BspTNI	GGTCTC	1 cut(s)	254
BsrBI	CCGCTC	4 cut(s)	189, 493, 619, 1368
BsrDI	GCAATG	1 cut(s)	1050
BsrFI	RCCGGY	3 cut(s)	26, 365, 443
BssAI	RCCGGY	3 cut(s)	26, 365, 443
BssECI	CCNNGG	6 cut(s)	163, 174, 220, 474, 866, 965
BssMI	GATC	9 cut(s)	40, 306, 348, 465, 676, 862, 895, 922, 1479
BssNI	GRCGYC	3 cut(s)	183, 234, 330
BssSI	CACGAG	1 cut(s)	245
BssT1I	CCWWGG	4 cut(s)	174, 474, 866, 965
Bst2BI	CACGAG	1 cut(s)	245
Bst2UI	CCWGG	1 cut(s)	425
Bst4CI	ACNGT	5 cut(s)	529, 542, 1035, 1151, 1230
Bst6I	CTCTTC	2 cut(s)	1092, 1164
BstACI	GRCGYC	3 cut(s)	183, 234, 330
BstAFI	CTTAAG	1 cut(s)	1403
BstDEI	CTNAG	3 cut(s)	735, 779, 1212
BstDSI	CCRYGG	3 cut(s)	174, 220, 965
BstEII	GGTNACC	1 cut(s)	523
BstF5I	GGATG	4 cut(s)	610, 955, 961, 975
BstFNI	CGCG	4 cut(s)	159, 210, 222, 398
BstHHI	GCGC	3 cut(s)	398, 443, 720
BstKTI	GATC	9 cut(s)	43, 309, 351, 468, 679, 865, 898, 925, 1482
BstMAI	GTCTC	2 cut(s)	254, 1219
BstMBI	GATC	9 cut(s)	40, 306, 348, 465, 676, 862, 895, 922, 1479
BstMCI	CGRYCG	3 cut(s)	157, 208, 220
BstMWI	GCNNNNNNNGC	8 cut(s)	12, 15, 32, 165, 216, 231, 447, 721
BstNI	CCWGG	1 cut(s)	425
BstPI	GGTNACC	1 cut(s)	523
BstSCI	CCNGG	3 cut(s)	95, 423, 658
BstSFI	CTRYAG	1 cut(s)	13
BstSLI	GKGCMC	1 cut(s)	483
BstUI	CGCG	4 cut(s)	159, 210, 222, 398
BstV1I	GCAGC	5 cut(s)	27, 280, 512, 702, 953
BstX2I	RGATCY	2 cut(s)	676, 895
BstXI	CCANNNNNNTGG	1 cut(s)	798
BstYI	RGATCY	2 cut(s)	676, 895
BstZI	CGGCCG	3 cut(s)	154, 205, 217
BtgI	CCRYGG	3 cut(s)	174, 220, 965
BtgZI	GCGATG	4 cut(s)	274, 454, 999, 1432
BtrI	CACGTC	2 cut(s)	64, 322
BtsCI	GGATG	4 cut(s)	610, 955, 961, 975
BtsI	GCAGTG	2 cut(s)	892, 951
BtsIMutI	CAGTG	3 cut(s)	892, 951, 1156
CfoI	GCGC	3 cut(s)	398, 443, 720
Cfr10I	RCCGGY	3 cut(s)	26, 365, 443
Cfr13I	GGNCC	5 cut(s)	99, 343, 406, 1183, 1324
Cfr42I	CCGCGG	1 cut(s)	223
CseI	GACGC	3 cut(s)	41, 148, 223
CsiI	ACCWGGT	1 cut(s)	423
Csp6I	GTAC	3 cut(s)	400, 1200, 1372
CviAII	CATG	6 cut(s)	175, 352, 430, 966, 1303, 1504
CviQI	GTAC	3 cut(s)	400, 1200, 1372
DdeI	CTNAG	3 cut(s)	735, 779, 1212
DpnI	GATC	9 cut(s)	42, 308, 350, 467, 678, 864, 897, 924, 1481
DpnII	GATC	9 cut(s)	40, 306, 348, 465, 676, 862, 895, 922, 1479
DraI	TTTAAA	1 cut(s)	1129
EaeI	YGGCCR	6 cut(s)	58, 154, 205, 217, 223, 367
EagI	CGGCCG	3 cut(s)	154, 205, 217
Eam1104I	CTCTTC	2 cut(s)	1092, 1164
EarI	CTCTTC	2 cut(s)	1092, 1164
EciI	GGCGGA	2 cut(s)	106, 323
Ecl136II	GAGCTC	3 cut(s)	214, 388, 436
EclXI	CGGCCG	3 cut(s)	154, 205, 217
Eco130I	CCWWGG	4 cut(s)	174, 474, 866, 965
Eco24I	GRGCYC	3 cut(s)	216, 390, 438
Eco31I	GGTCTC	1 cut(s)	254
Eco47I	GGWCC	3 cut(s)	343, 406, 1324
Eco52I	CGGCCG	3 cut(s)	154, 205, 217
Eco53kI	GAGCTC	3 cut(s)	214, 388, 436
Eco57I	CTGAAG	2 cut(s)	1116, 1239
Eco91I	GGTNACC	1 cut(s)	523
EcoICRI	GAGCTC	3 cut(s)	214, 388, 436
EcoO65I	GGTNACC	1 cut(s)	523
EcoRII	CCWGG	1 cut(s)	423
EcoT14I	CCWWGG	4 cut(s)	174, 474, 866, 965
EcoT22I	ATGCAT	1 cut(s)	991
EcoT38I	GRGCYC	3 cut(s)	216, 390, 438
ErhI	CCWWGG	4 cut(s)	174, 474, 866, 965
FaeI	CATG	6 cut(s)	178, 355, 433, 969, 1306, 1507
FalI	AAGNNNNNCTT	2 cut(s)	1170, 1202
FaqI	GGGAC	3 cut(s)	74, 581, 773
FatI	CATG	6 cut(s)	174, 351, 429, 965, 1302, 1503
FauI	CCCGC	4 cut(s)	53, 486, 1174, 1361
FbaI	TGATCA	1 cut(s)	306
FokI	GGATG	4 cut(s)	617, 948, 962, 982
FriOI	GRGCYC	3 cut(s)	216, 390, 438
FspBI	CTAG	1 cut(s)	1023
FspI	TGCGCA	1 cut(s)	719
GlaI	GCGC	3 cut(s)	397, 442, 719
GsaI	CCCAGC	1 cut(s)	1199
GsuI	CTGGAG	1 cut(s)	777
HapII	CCGG	6 cut(s)	27, 97, 366, 444, 578, 660
HgaI	GACGC	3 cut(s)	41, 148, 223
HhaI	GCGC	3 cut(s)	398, 443, 720
Hin1I	GRCGYC	3 cut(s)	183, 234, 330
Hin1II	CATG	6 cut(s)	178, 355, 433, 969, 1306, 1507
Hin6I	GCGC	3 cut(s)	396, 441, 718
HinP1I	GCGC	3 cut(s)	396, 441, 718
HincII	GTYRAC	2 cut(s)	421, 1459
HindII	GTYRAC	2 cut(s)	421, 1459
HindIII	AAGCTT	1 cut(s)	1205
HinfI	GANTC	6 cut(s)	250, 505, 611, 639, 786, 1214
HpaII	CCGG	6 cut(s)	27, 97, 366, 444, 578, 660
HphI	GGTGA	4 cut(s)	229, 355, 535, 1433
Hpy166II	GTNNAC	2 cut(s)	421, 1459
Hpy188I	TCNGA	9 cut(s)	300, 490, 519, 638, 862, 994, 1096, 1213, 1219
Hpy188III	TCNNGA	8 cut(s)	91, 247, 346, 1004, 1023, 1057, 1378, 1523
Hpy8I	GTNNAC	2 cut(s)	421, 1459
Hpy99I	CGWCG	5 cut(s)	35, 320, 323, 386, 533
HpyAV	CCTTC	6 cut(s)	211, 882, 1066, 1111, 1196, 1470
HpyCH4III	ACNGT	5 cut(s)	529, 542, 1035, 1151, 1230
HpyCH4IV	ACGT	4 cut(s)	63, 183, 321, 330
HpyCH4V	TGCA	9 cut(s)	15, 282, 355, 535, 941, 989, 1067, 1082, 1300
HpyF10VI	GCNNNNNNNGC	8 cut(s)	12, 15, 32, 165, 216, 231, 447, 721
HpyF3I	CTNAG	3 cut(s)	735, 779, 1212
HpySE526I	ACGT	4 cut(s)	63, 183, 321, 330
Hsp92I	GRCGYC	3 cut(s)	183, 234, 330
Hsp92II	CATG	6 cut(s)	178, 355, 433, 969, 1306, 1507
HspAI	GCGC	3 cut(s)	396, 441, 718
KpnI	GGTACC	1 cut(s)	1203
KroI	GCCGGC	2 cut(s)	26, 443
KroNI	GCCGGC	2 cut(s)	28, 445
Ksp22I	TGATCA	1 cut(s)	306
KspI	CCGCGG	1 cut(s)	223
Kzo9I	GATC	9 cut(s)	40, 306, 348, 465, 676, 862, 895, 922, 1479
LmnI	GCTCC	5 cut(s)	194, 433, 490, 731, 1393
Lsp1109I	GCAGC	5 cut(s)	27, 280, 512, 702, 953
LweI	GCATC	4 cut(s)	817, 960, 976, 998
MabI	ACCWGGT	1 cut(s)	423
MaeI	CTAG	1 cut(s)	1023
MaeII	ACGT	4 cut(s)	63, 183, 321, 330
MaeIII	GTNAC	6 cut(s)	235, 247, 523, 542, 842, 1531
MalI	GATC	9 cut(s)	42, 308, 350, 467, 678, 864, 897, 924, 1481
MbiI	CCGCTC	4 cut(s)	189, 493, 619, 1368
MboI	GATC	9 cut(s)	40, 306, 348, 465, 676, 862, 895, 922, 1479
MboII	GAAGA	8 cut(s)	643, 827, 916, 989, 1109, 1181, 1397, 1554
MfeI	CAATTG	1 cut(s)	1266
MflI	RGATCY	2 cut(s)	676, 895
MhlI	GDGCHC	4 cut(s)	216, 390, 438, 483
MluCI	AATT	7 cut(s)	227, 583, 1077, 1158, 1266, 1388, 1526
MlyI	GAGTC	5 cut(s)	244, 605, 633, 795, 1223
MmeI	TCCRAC	1 cut(s)	78
Mph1103I	ATGCAT	1 cut(s)	991
MreI	CGCCGGCG	2 cut(s)	26, 443
MroNI	GCCGGC	2 cut(s)	26, 443
MroXI	GAANNNNTTC	1 cut(s)	1034
MseI	TTAA	5 cut(s)	957, 1128, 1175, 1404, 1437
MslI	CAYNNNNRTG	1 cut(s)	1379
MspA1I	CMGCKG	1 cut(s)	222
MspCI	CTTAAG	1 cut(s)	1403
MspI	CCGG	6 cut(s)	27, 97, 366, 444, 578, 660
MspR9I	CCNGG	3 cut(s)	97, 425, 660
MunI	CAATTG	1 cut(s)	1266
MvaI	CCWGG	1 cut(s)	425
MvnI	CGCG	4 cut(s)	159, 210, 222, 398
MwoI	GCNNNNNNNGC	8 cut(s)	12, 15, 32, 165, 216, 231, 447, 721
NaeI	GCCGGC	2 cut(s)	28, 445
NciI	CCSGG	2 cut(s)	97, 660
NcoI	CCATGG	2 cut(s)	174, 965
NdeII	GATC	9 cut(s)	40, 306, 348, 465, 676, 862, 895, 922, 1479
NgoMIV	GCCGGC	2 cut(s)	26, 443
NlaIII	CATG	6 cut(s)	178, 355, 433, 969, 1306, 1507
NlaIV	GGNNCC	1 cut(s)	1201
NmeAIII	GCCGAG	2 cut(s)	144, 195
NmuCI	GTSAC	6 cut(s)	235, 247, 523, 542, 842, 1531
NsbI	TGCGCA	1 cut(s)	719
NsiI	ATGCAT	1 cut(s)	991
PcsI	WCGNNNNNNNCGW	2 cut(s)	327, 642
PdiI	GCCGGC	2 cut(s)	28, 445
PdmI	GAANNNNTTC	1 cut(s)	1034
PfeI	GAWTC	1 cut(s)	505
Pfl23II	CGTACG	1 cut(s)	399
PleI	GAGTC	5 cut(s)	244, 605, 633, 794, 1222
PpsI	GAGTC	5 cut(s)	244, 605, 633, 794, 1222
PsiI	TTATAA	1 cut(s)	1286
Psp124BI	GAGCTC	3 cut(s)	216, 390, 438
Psp6I	CCWGG	1 cut(s)	423
PspEI	GGTNACC	1 cut(s)	523
PspFI	CCCAGC	1 cut(s)	1195
PspGI	CCWGG	1 cut(s)	423
PspLI	CGTACG	1 cut(s)	399
PspN4I	GGNNCC	1 cut(s)	1201
PspPI	GGNCC	5 cut(s)	99, 343, 406, 1183, 1324
PstI	CTGCAG	1 cut(s)	17
PsuI	RGATCY	2 cut(s)	676, 895
RsaI	GTAC	3 cut(s)	401, 1201, 1373
RsaNI	GTAC	3 cut(s)	400, 1200, 1372
RseI	CAYNNNNRTG	1 cut(s)	1379
SacI	GAGCTC	3 cut(s)	216, 390, 438
SacII	CCGCGG	1 cut(s)	223
SaqAI	TTAA	5 cut(s)	957, 1128, 1175, 1404, 1437
Sau3AI	GATC	9 cut(s)	40, 306, 348, 465, 676, 862, 895, 922, 1479
Sau96I	GGNCC	5 cut(s)	99, 343, 406, 1183, 1324
SchI	GAGTC	5 cut(s)	244, 605, 633, 795, 1223
ScrFI	CCNGG	3 cut(s)	97, 425, 660
SduI	GDGCHC	4 cut(s)	216, 390, 438, 483
SexAI	ACCWGGT	1 cut(s)	423
SfaNI	GCATC	4 cut(s)	817, 960, 976, 998
SfcI	CTRYAG	1 cut(s)	13
Sfr303I	CCGCGG	1 cut(s)	223
SgrAI	CRCCGGYG	2 cut(s)	26, 443
SgrBI	CCGCGG	1 cut(s)	223
SinI	GGWCC	3 cut(s)	343, 406, 1324
SmiMI	CAYNNNNRTG	1 cut(s)	1379
SmlI	CTYRAG	3 cut(s)	620, 1055, 1403
SmoI	CTYRAG	3 cut(s)	620, 1055, 1403
Sse9I	AATT	7 cut(s)	227, 583, 1077, 1158, 1266, 1388, 1526
SspI	AATATT	1 cut(s)	1349
SspMI	CTAG	1 cut(s)	1023
SstI	GAGCTC	3 cut(s)	216, 390, 438
StyD4I	CCNGG	3 cut(s)	95, 423, 658
StyI	CCWWGG	4 cut(s)	174, 474, 866, 965
TaaI	ACNGT	5 cut(s)	529, 542, 1035, 1151, 1230
TaiI	ACGT	4 cut(s)	66, 186, 324, 333
TaqI	TCGA	4 cut(s)	384, 459, 916, 1353
TaqII	GACCGA	1 cut(s)	406
TasI	AATT	7 cut(s)	227, 583, 1077, 1158, 1266, 1388, 1526
TfiI	GAWTC	1 cut(s)	505
Tru1I	TTAA	5 cut(s)	957, 1128, 1175, 1404, 1437
Tru9I	TTAA	5 cut(s)	957, 1128, 1175, 1404, 1437
TscAI	CASTG	3 cut(s)	892, 951, 1156
TseFI	GTSAC	6 cut(s)	235, 247, 523, 542, 842, 1531
TseI	GCWGC	5 cut(s)	15, 268, 500, 715, 941
Tsp45I	GTSAC	6 cut(s)	235, 247, 523, 542, 842, 1531
TspDTI	ATGAA	6 cut(s)	825, 846, 1043, 1061, 1319, 1440
TspGWI	ACGGA	1 cut(s)	634
TspRI	CASTG	3 cut(s)	892, 951, 1156
Vha464I	CTTAAG	1 cut(s)	1403
VpaK11BI	GGWCC	3 cut(s)	343, 406, 1324
XapI	RAATTY	1 cut(s)	583
XbaI	TCTAGA	1 cut(s)	1022
XmnI	GAANNNNTTC	1 cut(s)	1034
XspI	CTAG	1 cut(s)	1023
ZraI	GACGTC	2 cut(s)	184, 331
Zsp2I	ATGCAT	1 cut(s)	991

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.