Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0014884

Physical Location & Seq

Genomic Coordinates Reverse (-)

18824267 .. 18829500

Gene Structure 5234 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1116100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGGGTAACAACTGCAGTAGCGGTACTCTCCCCTCCTCCGCCGAAGACCCCGCCGACCACCACCACCGCCCATCAAACGGCGCCGTTCGCATCCTCCCTCCCAATGCCTCCGCCCCATCCAAACCCAAACCCCACACACCCCCAACCACCACCTCCGCTTCCGGCACCGCTCCCATCGGCCGGGTCCTGGGCCGGCCCATGGAAGACGTCCGGTCCTCCTACACCTTCGGCCGTGAGCTCGGCCGCGGCCAATTCGGCGTCACCTACCTCGTCACCGATAAGCAGACAAAGCAGCTGTTCGCCTGCAAATCCATCGCCACCCGCAAGCTCATCAACCACGACGACGTCGAGGATGTCCGCCGCGAGGTCCAGATCATGCACCACCTCACCGGGCACCGCAACATCGTGGAGCTCAAGGGAGCTTACGAGGACCGCCACTCCGTGAACCTGGTCATGGAGCTCTGCGCCGGCGGCGAGCTCTTCGATCGGATCATGGCTAAAGGCCACTACTCCGAGCGAGCTGCGGCCAATCTCTGCCGGCAGATCGTCACCGTGGTTCACTACTGTCACTCGATGGGGGTTATGCATAGGGATTTGAAGCCGGAGAATTTCTTGCTTTTGAGCAAGGACGAGGACTCGCCGCTCAAGGCCACCGATTTCGGGCTCTCGGTGTTCTTTAAGCCAGGAGATGTATTTAAGGATCTTGTGGGAAGTGCATATTATGTTGCTCCTGAAGTGCTGCGTCGTCGTTATGGAGCTGAGGCTGATATTTGGAGTGCAGGGGTGATACTATACATTCTACTTAGTGGGGTCCCACCATTCTGGGGAGAGAATGAACAAGGTATCTTTGATGCTATCCTTCGGGGACATCTTGACTTTGTGTCTGATCCTTGGCCTTCCATTTCCAGCAGTGCCAAAGATCTTGTGAAGAAAATGCTCCGAGCTGATCCTAAGGAACGACTTACAGCAGCTGACGTCCTGAGTCATCCATGGATGCGGGAAGATGGTGATGCATCTGATAAACCTCTTGATAGTGCTGTTCTATCTAGAATGAAACAGTTCAGGGCAATGAACAAACTCAAGAAAGTAGCATTGAAGGTAATTGCAGAAAATCTTTCTGAAGAAGAAATCATAGGCTTGAAGGAAATGTTCAAATCCATGGACACAGATAACAGTGGAACAATTACATATGAAGAGTTAAAAGCTGGCCTTCCGAAAATGGGTACCAAGCTCTCTGAGTCTGAAGTGAGACAGTTGATGGAAGCGGCTGATGTCGATGGAAATGGAACAATTGACTACATCGAGTTTATAACAGCTACCATGCACATGAATAGAATGGAAAGAGAGGATCATTTATACAAAGCCTTTGAATATTTTGACGAAGACAAGAGCGGGTACATCACGGTGGAAGAGTTGGAGCAAGCGCTTAAGAAGTATAATATGGGTGATGAGAAAACAATCAAGGAAATCATTGCAGAAGTTGACACAGATTGTGATGGAAGGATTAACTATGATGAGTTTGCAGCCATGATGAGGAAAGGCAATCCTGAGTTGGTCACAAATAGAAGAAGAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010119 GO:0010359 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

526

Amino Acids

58.88

Weight (kDa)

5.91

Isoelectric Point (pI)

39.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	74 - 332	4.8e-78	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	75 - 327	1.8e-29	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	380 - 440	8.8e-14	EF-hand domain pair
EF-hand_1	PF00036	380 - 406	1.4e-07	EF hand domain
EF-hand_6	PF13405	380 - 408	2.9e-06	EF-hand domain
EF-hand_8	PF13833	392 - 439	8.7e-06	EF-hand domain pair
EF-hand_7	PF13499	449 - 512	3.3e-17	EF-hand domain pair
EF-hand_1	PF00036	452 - 478	1.9e-06	EF hand domain
EF-hand_6	PF13405	452 - 479	5.1e-07	EF-hand domain
EF-hand_8	PF13833	464 - 513	8.6e-08	EF-hand domain pair
EF-hand_1	PF00036	487 - 513	2e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014884)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23650
fragaria_vesca	FvH4_5g03610 FvH4_5g03610 FvH4_5g03610
malus_domestica	MD06G1095900.v1.1 MD14G1116100.v1.1
prunus_persica	Prupe.5G110500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0196111
rosa_laevigata	RLG00000004064
rosa_multiflora	Rmu_sc0013391.1_g000002
rosa_roxburghii	Rroxscaffold_3G00259800
rosa_rugosa	Rorug07G0030700
rosa_samantha	Rh7AG156500 Rh7BG158100 Rh7CG162000
rosa_wichuraiana	Rw7G013370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1310
AatII	GACGTC	3 cut(s)	210, 348, 978
Acc65I	GGTACC	1 cut(s)	1223
AccB1I	GGYRCC	4 cut(s)	80, 164, 393, 1223
AccBSI	CCGCTC	3 cut(s)	170, 643, 1392
AccII	CGCG	2 cut(s)	246, 363
AclWI	GGATC	5 cut(s)	497, 708, 881, 941, 1356
AcoI	YGGCCR	5 cut(s)	178, 229, 241, 247, 525
AcsI	RAATTY	1 cut(s)	607
AcuI	CTGAAG	3 cut(s)	753, 1140, 1263
AcyI	GRCGYC	5 cut(s)	81, 207, 258, 345, 975
AdeI	CACNNNGTG	1 cut(s)	442
AfaI	GTAC	3 cut(s)	25, 1225, 1397
AfeI	AGCGCT	1 cut(s)	1425
AflII	CTTAAG	1 cut(s)	1427
AgsI	TTSAA	5 cut(s)	598, 1096, 1141, 1153, 1370
AhdI	GACNNNNNGTC	2 cut(s)	211, 880
AjnI	CCWGG	3 cut(s)	186, 447, 682
Alw21I	GWGCWC	4 cut(s)	240, 414, 462, 480
Alw26I	GTCTC	1 cut(s)	1243
AlwI	GGATC	5 cut(s)	497, 708, 881, 941, 1356
AlwNI	CAGNNNCTG	1 cut(s)	971
Aor51HI	AGCGCT	1 cut(s)	1425
ApeKI	GCWGC	5 cut(s)	292, 521, 739, 968, 1523
ApoI	RAATTY	1 cut(s)	607
Asp700I	GAANNNNTTC	1 cut(s)	1058
Asp718I	GGTACC	1 cut(s)	1223
AspLEI	GCGC	3 cut(s)	83, 467, 1426
AspS9I	GGNCC	7 cut(s)	184, 190, 195, 213, 367, 430, 811
AsuC2I	CCSGG	2 cut(s)	182, 391
AsuHPI	GGTGA	7 cut(s)	253, 265, 379, 541, 796, 1019, 1457
AvaII	GGWCC	5 cut(s)	184, 213, 367, 430, 811
AxyI	CCTNAGG	1 cut(s)	951
BaeGI	GKGCMC	1 cut(s)	396
BanI	GGYRCC	4 cut(s)	80, 164, 393, 1223
BanII	GRGCYC	5 cut(s)	240, 414, 462, 480, 666
BbsI	GAAGAC	3 cut(s)	51, 210, 1389
Bbv12I	GWGCWC	4 cut(s)	240, 414, 462, 480
BbvCI	CCTCAGC	1 cut(s)	759
BbvI	GCAGC	5 cut(s)	304, 508, 726, 980, 1535
BccI	CCATC	9 cut(s)	79, 124, 182, 320, 568, 998, 1252, 1271, 1490
BceAI	ACGGC	3 cut(s)	68, 94, 216
BcgI	CGANNNNNNTGC	4 cut(s)	295, 329, 503, 537
BciT130I	CCWGG	3 cut(s)	188, 449, 684
BcnI	CCSGG	2 cut(s)	182, 391
BcoDI	GTCTC	1 cut(s)	1243
BfaI	CTAG	1 cut(s)	1047
BfmI	CTRYAG	1 cut(s)	13
BfoI	RGCGCY	2 cut(s)	84, 1427
BfrI	CTTAAG	1 cut(s)	1427
BglI	GCCNNNNNGGC	1 cut(s)	255
BglII	AGATCT	1 cut(s)	919
Bme1390I	CCNGG	5 cut(s)	182, 188, 391, 449, 684
Bme18I	GGWCC	5 cut(s)	184, 213, 367, 430, 811
BmeRI	GACNNNNNGTC	2 cut(s)	211, 880
BmgT120I	GGNCC	7 cut(s)	184, 190, 195, 213, 367, 430, 811
BmiI	GGNNCC	7 cut(s)	82, 166, 185, 395, 812, 813, 1225
BmrFI	CCNGG	5 cut(s)	182, 188, 391, 449, 684
BmsI	GCATC	5 cut(s)	99, 841, 984, 1000, 1022
BpiI	GAAGAC	3 cut(s)	51, 210, 1389
Bpu10I	CCTNAGC	1 cut(s)	759
BpuEI	CTTGAG	3 cut(s)	398, 629, 1064
BpuMI	CCSGG	2 cut(s)	182, 391
BsaHI	GRCGYC	5 cut(s)	81, 207, 258, 345, 975
BsaJI	CCNNGG	7 cut(s)	187, 198, 244, 552, 890, 989, 1158
BsaWI	WCCGGW	1 cut(s)	210
BsaXI	ACNNNNNCTCC	2 cut(s)	422, 452
Bse118I	RCCGGY	3 cut(s)	192, 467, 537
Bse21I	CCTNAGG	1 cut(s)	951
Bse3DI	GCAATG	2 cut(s)	1074, 1470
BseBI	CCWGG	3 cut(s)	188, 449, 684
BseDI	CCNNGG	7 cut(s)	187, 198, 244, 552, 890, 989, 1158
BseGI	GGATG	5 cut(s)	90, 116, 358, 985, 999
BseMI	GCAATG	2 cut(s)	1074, 1470
BseMII	CTCAG	4 cut(s)	750, 971, 1227, 1539
BseRI	GAGGAG	1 cut(s)	25
BseSI	GKGCMC	1 cut(s)	396
BseX3I	CGGCCG	3 cut(s)	178, 229, 241
BseXI	GCAGC	5 cut(s)	304, 508, 726, 980, 1535
BsgI	GTGCAG	1 cut(s)	798
Bsh1236I	CGCG	2 cut(s)	246, 363
Bsh1285I	CGRYCG	4 cut(s)	181, 232, 244, 487
BshNI	GGYRCC	4 cut(s)	80, 164, 393, 1223
BsiEI	CGRYCG	4 cut(s)	181, 232, 244, 487
BsiHKAI	GWGCWC	4 cut(s)	240, 414, 462, 480
BsiSI	CCGG	8 cut(s)	162, 181, 193, 211, 390, 468, 538, 602
BslFI	GGGAC	2 cut(s)	797, 879
BsmAI	GTCTC	1 cut(s)	1243
BsmFI	GGGAC	2 cut(s)	797, 879
Bsp1286I	GDGCHC	6 cut(s)	240, 396, 414, 462, 480, 666
Bsp143I	GATC	9 cut(s)	372, 484, 489, 543, 700, 886, 919, 946, 1348
Bsp19I	CCATGG	3 cut(s)	198, 989, 1158
BspCNI	CTCAG	4 cut(s)	751, 972, 1228, 1540
BspFNI	CGCG	2 cut(s)	246, 363
BspLI	GGNNCC	7 cut(s)	82, 166, 185, 395, 812, 813, 1225
BspMAI	CTGCAG	1 cut(s)	17
BspPI	GGATC	5 cut(s)	497, 708, 881, 941, 1356
BspQI	GCTCTTC	1 cut(s)	485
BspT107I	GGYRCC	4 cut(s)	80, 164, 393, 1223
BspTI	CTTAAG	1 cut(s)	1427
BsrBI	CCGCTC	3 cut(s)	170, 643, 1392
BsrDI	GCAATG	2 cut(s)	1074, 1470
BsrFI	RCCGGY	3 cut(s)	192, 467, 537
BssAI	RCCGGY	3 cut(s)	192, 467, 537
BssECI	CCNNGG	7 cut(s)	187, 198, 244, 552, 890, 989, 1158
BssMI	GATC	9 cut(s)	372, 484, 489, 543, 700, 886, 919, 946, 1348
BssNI	GRCGYC	5 cut(s)	81, 207, 258, 345, 975
BssT1I	CCWWGG	4 cut(s)	198, 890, 989, 1158
Bst2UI	CCWGG	3 cut(s)	188, 449, 684
Bst4CI	ACNGT	6 cut(s)	553, 566, 1059, 1175, 1254, 1405
Bst6I	CTCTTC	3 cut(s)	485, 1188, 1404
BstACI	GRCGYC	5 cut(s)	81, 207, 258, 345, 975
BstAFI	CTTAAG	1 cut(s)	1427
BstC8I	GCNNGC	9 cut(s)	194, 304, 326, 469, 476, 519, 539, 1207, 1422
BstDEI	CTNAG	6 cut(s)	759, 803, 951, 980, 1236, 1548
BstDSI	CCRYGG	5 cut(s)	198, 244, 552, 989, 1158
BstF5I	GGATG	5 cut(s)	90, 116, 358, 985, 999
BstFNI	CGCG	2 cut(s)	246, 363
BstH2I	RGCGCY	2 cut(s)	84, 1427
BstHHI	GCGC	3 cut(s)	83, 467, 1426
BstKTI	GATC	9 cut(s)	375, 487, 492, 546, 703, 889, 922, 949, 1351
BstMAI	GTCTC	1 cut(s)	1243
BstMBI	GATC	9 cut(s)	372, 484, 489, 543, 700, 886, 919, 946, 1348
BstMCI	CGRYCG	4 cut(s)	181, 232, 244, 487
BstMWI	GCNNNNNNNGC	4 cut(s)	87, 255, 289, 471
BstNI	CCWGG	3 cut(s)	188, 449, 684
BstSCI	CCNGG	5 cut(s)	180, 186, 389, 447, 682
BstSFI	CTRYAG	1 cut(s)	13
BstSLI	GKGCMC	1 cut(s)	396
BstUI	CGCG	2 cut(s)	246, 363
BstV1I	GCAGC	5 cut(s)	304, 508, 726, 980, 1535
BstV2I	GAAGAC	3 cut(s)	51, 210, 1389
BstX2I	RGATCY	2 cut(s)	700, 919
BstXI	CCANNNNNNTGG	1 cut(s)	822
BstYI	RGATCY	2 cut(s)	700, 919
BstZI	CGGCCG	3 cut(s)	178, 229, 241
Bsu36I	CCTNAGG	1 cut(s)	951
BtgI	CCRYGG	5 cut(s)	198, 244, 552, 989, 1158
BtgZI	GCGATG	1 cut(s)	298
BtsCI	GGATG	5 cut(s)	90, 116, 358, 985, 999
BtsI	GCAGTG	1 cut(s)	916
BtsIMutI	CAGTG	2 cut(s)	916, 1180
Cac8I	GCNNGC	9 cut(s)	194, 304, 326, 469, 476, 519, 539, 1207, 1422
CaiI	CAGNNNCTG	1 cut(s)	971
CfoI	GCGC	3 cut(s)	83, 467, 1426
Cfr10I	RCCGGY	3 cut(s)	192, 467, 537
Cfr13I	GGNCC	7 cut(s)	184, 190, 195, 213, 367, 430, 811
Cfr42I	CCGCGG	1 cut(s)	247
CseI	GACGC	2 cut(s)	247, 731
CsiI	ACCWGGT	1 cut(s)	447
Csp6I	GTAC	3 cut(s)	24, 1224, 1396
CviAII	CATG	9 cut(s)	199, 376, 454, 493, 990, 1159, 1321, 1327, 1528
CviQI	GTAC	3 cut(s)	24, 1224, 1396
DdeI	CTNAG	6 cut(s)	759, 803, 951, 980, 1236, 1548
DinI	GGCGCC	1 cut(s)	82
DpnI	GATC	9 cut(s)	374, 486, 491, 545, 702, 888, 921, 948, 1350
DpnII	GATC	9 cut(s)	372, 484, 489, 543, 700, 886, 919, 946, 1348
DraIII	CACNNNGTG	1 cut(s)	442
DriI	GACNNNNNGTC	2 cut(s)	211, 880
EaeI	YGGCCR	5 cut(s)	178, 229, 241, 247, 525
EagI	CGGCCG	3 cut(s)	178, 229, 241
Eam1104I	CTCTTC	3 cut(s)	485, 1188, 1404
Eam1105I	GACNNNNNGTC	2 cut(s)	211, 880
EarI	CTCTTC	3 cut(s)	485, 1188, 1404
EciI	GGCGGA	3 cut(s)	28, 100, 347
Ecl136II	GAGCTC	4 cut(s)	238, 412, 460, 478
EclXI	CGGCCG	3 cut(s)	178, 229, 241
Eco130I	CCWWGG	4 cut(s)	198, 890, 989, 1158
Eco24I	GRGCYC	5 cut(s)	240, 414, 462, 480, 666
Eco47I	GGWCC	5 cut(s)	184, 213, 367, 430, 811
Eco47III	AGCGCT	1 cut(s)	1425
Eco52I	CGGCCG	3 cut(s)	178, 229, 241
Eco53kI	GAGCTC	4 cut(s)	238, 412, 460, 478
Eco57I	CTGAAG	3 cut(s)	753, 1140, 1263
Eco81I	CCTNAGG	1 cut(s)	951
EcoICRI	GAGCTC	4 cut(s)	238, 412, 460, 478
EcoO109I	RGGNCCY	2 cut(s)	184, 811
EcoRII	CCWGG	3 cut(s)	186, 447, 682
EcoT14I	CCWWGG	4 cut(s)	198, 890, 989, 1158
EcoT22I	ATGCAT	2 cut(s)	588, 1015
EcoT38I	GRGCYC	5 cut(s)	240, 414, 462, 480, 666
EgeI	GGCGCC	1 cut(s)	82
EheI	GGCGCC	1 cut(s)	82
ErhI	CCWWGG	4 cut(s)	198, 890, 989, 1158
FaeI	CATG	9 cut(s)	202, 379, 457, 496, 993, 1162, 1324, 1330, 1531
FalI	AAGNNNNNCTT	2 cut(s)	1194, 1226
FaqI	GGGAC	2 cut(s)	797, 879
FatI	CATG	9 cut(s)	198, 375, 453, 492, 989, 1158, 1320, 1326, 1527
FauI	CCCGC	4 cut(s)	58, 329, 990, 1385
FauNDI	CATATG	1 cut(s)	1189
FokI	GGATG	5 cut(s)	77, 103, 365, 972, 1006
FriOI	GRGCYC	5 cut(s)	240, 414, 462, 480, 666
FseI	GGCCGGCC	1 cut(s)	196
FspBI	CTAG	1 cut(s)	1047
GlaI	GCGC	3 cut(s)	82, 466, 1425
HaeII	RGCGCY	2 cut(s)	84, 1427
HapII	CCGG	8 cut(s)	162, 181, 193, 211, 390, 468, 538, 602
HgaI	GACGC	2 cut(s)	247, 731
HhaI	GCGC	3 cut(s)	83, 467, 1426
Hin1I	GRCGYC	5 cut(s)	81, 207, 258, 345, 975
Hin1II	CATG	9 cut(s)	202, 379, 457, 496, 993, 1162, 1324, 1330, 1531
Hin6I	GCGC	3 cut(s)	81, 465, 1424
HinP1I	GCGC	3 cut(s)	81, 465, 1424
HincII	GTYRAC	1 cut(s)	1483
HindII	GTYRAC	1 cut(s)	1483
HinfI	GANTC	3 cut(s)	635, 982, 1238
HpaII	CCGG	8 cut(s)	162, 181, 193, 211, 390, 468, 538, 602
HphI	GGTGA	7 cut(s)	253, 265, 379, 541, 796, 1019, 1457
Hpy166II	GTNNAC	3 cut(s)	445, 559, 1483
Hpy188I	TCNGA	9 cut(s)	489, 514, 886, 941, 1018, 1120, 1215, 1237, 1243
Hpy188III	TCNNGA	8 cut(s)	370, 731, 872, 979, 1028, 1047, 1081, 1547
Hpy8I	GTNNAC	3 cut(s)	445, 559, 1483
Hpy99I	CGWCG	5 cut(s)	344, 347, 350, 747, 750
HpyAV	CCTTC	7 cut(s)	235, 869, 906, 1090, 1135, 1220, 1494
HpyCH4III	ACNGT	6 cut(s)	553, 566, 1059, 1175, 1254, 1405
HpyCH4IV	ACGT	3 cut(s)	207, 345, 975
HpyF10VI	GCNNNNNNNGC	4 cut(s)	87, 255, 289, 471
HpyF3I	CTNAG	6 cut(s)	759, 803, 951, 980, 1236, 1548
HpySE526I	ACGT	3 cut(s)	207, 345, 975
Hsp92I	GRCGYC	5 cut(s)	81, 207, 258, 345, 975
Hsp92II	CATG	9 cut(s)	202, 379, 457, 496, 993, 1162, 1324, 1330, 1531
HspAI	GCGC	3 cut(s)	81, 465, 1424
KasI	GGCGCC	1 cut(s)	80
KflI	GGGWCCC	1 cut(s)	811
KpnI	GGTACC	1 cut(s)	1227
KroI	GCCGGC	3 cut(s)	192, 467, 537
KroNI	GCCGGC	3 cut(s)	194, 469, 539
KspI	CCGCGG	1 cut(s)	247
Kzo9I	GATC	9 cut(s)	372, 484, 489, 543, 700, 886, 919, 946, 1348
LguI	GCTCTTC	1 cut(s)	485
LmnI	GCTCC	8 cut(s)	175, 409, 419, 457, 733, 755, 942, 1417
Lsp1109I	GCAGC	5 cut(s)	304, 508, 726, 980, 1535
LweI	GCATC	5 cut(s)	99, 841, 984, 1000, 1022
MabI	ACCWGGT	1 cut(s)	447
MaeI	CTAG	1 cut(s)	1047
MaeII	ACGT	3 cut(s)	207, 345, 975
MaeIII	GTNAC	6 cut(s)	5, 259, 271, 547, 566, 1555
MalI	GATC	9 cut(s)	374, 486, 491, 545, 702, 888, 921, 948, 1350
MbiI	CCGCTC	3 cut(s)	170, 643, 1392
MboI	GATC	9 cut(s)	372, 484, 489, 543, 700, 886, 919, 946, 1348
MfeI	CAATTG	1 cut(s)	1290
MflI	RGATCY	2 cut(s)	700, 919
MhlI	GDGCHC	6 cut(s)	240, 396, 414, 462, 480, 666
MluCI	AATT	5 cut(s)	251, 607, 1101, 1182, 1290
Mly113I	GGCGCC	1 cut(s)	81
MlyI	GAGTC	3 cut(s)	629, 991, 1247
MmeI	TCCRAC	1 cut(s)	1395
Mph1103I	ATGCAT	2 cut(s)	588, 1015
MreI	CGCCGGCG	1 cut(s)	467
MroNI	GCCGGC	3 cut(s)	192, 467, 537
MroXI	GAANNNNTTC	1 cut(s)	1058
MseI	TTAA	5 cut(s)	678, 696, 1199, 1428, 1506
MslI	CAYNNNNRTG	2 cut(s)	1325, 1403
MspA1I	CMGCKG	3 cut(s)	246, 295, 971
MspCI	CTTAAG	1 cut(s)	1427
MspI	CCGG	8 cut(s)	162, 181, 193, 211, 390, 468, 538, 602
MspR9I	CCNGG	5 cut(s)	182, 188, 391, 449, 684
MunI	CAATTG	1 cut(s)	1290
MvaI	CCWGG	3 cut(s)	188, 449, 684
MvnI	CGCG	2 cut(s)	246, 363
MwoI	GCNNNNNNNGC	4 cut(s)	87, 255, 289, 471
NaeI	GCCGGC	3 cut(s)	194, 469, 539
NarI	GGCGCC	1 cut(s)	81
NciI	CCSGG	2 cut(s)	182, 391
NcoI	CCATGG	3 cut(s)	198, 989, 1158
NdeI	CATATG	1 cut(s)	1189
NdeII	GATC	9 cut(s)	372, 484, 489, 543, 700, 886, 919, 946, 1348
NgoMIV	GCCGGC	3 cut(s)	192, 467, 537
NlaIII	CATG	9 cut(s)	202, 379, 457, 496, 993, 1162, 1324, 1330, 1531
NlaIV	GGNNCC	7 cut(s)	82, 166, 185, 395, 812, 813, 1225
NmeAIII	GCCGAG	1 cut(s)	219
NmuCI	GTSAC	5 cut(s)	259, 271, 547, 566, 1555
NsiI	ATGCAT	2 cut(s)	588, 1015
PciSI	GCTCTTC	1 cut(s)	485
PcsI	WCGNNNNNNNCGW	1 cut(s)	345
PdiI	GCCGGC	3 cut(s)	194, 469, 539
PdmI	GAANNNNTTC	1 cut(s)	1058
PflFI	GACNNNGTC	1 cut(s)	344
Ple19I	CGATCG	1 cut(s)	487
PleI	GAGTC	3 cut(s)	629, 990, 1246
PluTI	GGCGCC	1 cut(s)	84
PpsI	GAGTC	3 cut(s)	629, 990, 1246
PpuMI	RGGWCCY	2 cut(s)	184, 811
PsiI	TTATAA	1 cut(s)	1310
Psp124BI	GAGCTC	4 cut(s)	240, 414, 462, 480
Psp5II	RGGWCCY	2 cut(s)	184, 811
Psp6I	CCWGG	3 cut(s)	186, 447, 682
PspGI	CCWGG	3 cut(s)	186, 447, 682
PspN4I	GGNNCC	7 cut(s)	82, 166, 185, 395, 812, 813, 1225
PspPI	GGNCC	7 cut(s)	184, 190, 195, 213, 367, 430, 811
PspPPI	RGGWCCY	2 cut(s)	184, 811
PstI	CTGCAG	1 cut(s)	17
PstNI	CAGNNNCTG	1 cut(s)	971
PsuI	RGATCY	2 cut(s)	700, 919
PsyI	GACNNNGTC	1 cut(s)	344
PvuI	CGATCG	1 cut(s)	487
PvuII	CAGCTG	2 cut(s)	295, 971
RigI	GGCCGGCC	1 cut(s)	196
RsaI	GTAC	3 cut(s)	25, 1225, 1397
RsaNI	GTAC	3 cut(s)	24, 1224, 1396
RseI	CAYNNNNRTG	2 cut(s)	1325, 1403
SacI	GAGCTC	4 cut(s)	240, 414, 462, 480
SacII	CCGCGG	1 cut(s)	247
SapI	GCTCTTC	1 cut(s)	485
SaqAI	TTAA	5 cut(s)	678, 696, 1199, 1428, 1506
Sau3AI	GATC	9 cut(s)	372, 484, 489, 543, 700, 886, 919, 946, 1348
Sau96I	GGNCC	7 cut(s)	184, 190, 195, 213, 367, 430, 811
SchI	GAGTC	3 cut(s)	629, 991, 1247
ScrFI	CCNGG	5 cut(s)	182, 188, 391, 449, 684
SduI	GDGCHC	6 cut(s)	240, 396, 414, 462, 480, 666
SexAI	ACCWGGT	1 cut(s)	447
SfaNI	GCATC	5 cut(s)	99, 841, 984, 1000, 1022
SfcI	CTRYAG	1 cut(s)	13
SfoI	GGCGCC	1 cut(s)	82
Sfr303I	CCGCGG	1 cut(s)	247
SgrAI	CRCCGGYG	1 cut(s)	467
SgrBI	CCGCGG	1 cut(s)	247
SinI	GGWCC	5 cut(s)	184, 213, 367, 430, 811
SmiMI	CAYNNNNRTG	2 cut(s)	1325, 1403
SmlI	CTYRAG	4 cut(s)	413, 644, 1079, 1427
SmoI	CTYRAG	4 cut(s)	413, 644, 1079, 1427
Sse9I	AATT	5 cut(s)	251, 607, 1101, 1182, 1290
SspDI	GGCGCC	1 cut(s)	80
SspI	AATATT	1 cut(s)	1373
SspMI	CTAG	1 cut(s)	1047
SstI	GAGCTC	4 cut(s)	240, 414, 462, 480
StyD4I	CCNGG	5 cut(s)	180, 186, 389, 447, 682
StyI	CCWWGG	4 cut(s)	198, 890, 989, 1158
TaaI	ACNGT	6 cut(s)	553, 566, 1059, 1175, 1254, 1405
TaiI	ACGT	3 cut(s)	210, 348, 978
TaqI	TCGA	5 cut(s)	348, 483, 572, 1275, 1302
TasI	AATT	5 cut(s)	251, 607, 1101, 1182, 1290
TauI	GCSGC	7 cut(s)	246, 249, 363, 474, 527, 643, 1268
Tru1I	TTAA	5 cut(s)	678, 696, 1199, 1428, 1506
Tru9I	TTAA	5 cut(s)	678, 696, 1199, 1428, 1506
TscAI	CASTG	2 cut(s)	916, 1180
TseFI	GTSAC	5 cut(s)	259, 271, 547, 566, 1555
TseI	GCWGC	5 cut(s)	292, 521, 739, 968, 1523
Tsp45I	GTSAC	5 cut(s)	259, 271, 547, 566, 1555
TspDTI	ATGAA	5 cut(s)	849, 1067, 1085, 1206, 1343
TspGWI	ACGGA	1 cut(s)	430
TspRI	CASTG	2 cut(s)	916, 1180
Tth111I	GACNNNGTC	1 cut(s)	344
Vha464I	CTTAAG	1 cut(s)	1427
VpaK11BI	GGWCC	5 cut(s)	184, 213, 367, 430, 811
XapI	RAATTY	1 cut(s)	607
XbaI	TCTAGA	1 cut(s)	1046
XmnI	GAANNNNTTC	1 cut(s)	1058
XspI	CTAG	1 cut(s)	1047
ZraI	GACGTC	3 cut(s)	208, 346, 976
Zsp2I	ATGCAT	2 cut(s)	588, 1015

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.