Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0014884

Physical Location & Seq

Genomic Coordinates Forward (+)

12735795 .. 12739791

Gene Structure 3997 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG156500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1404 bp

ATGGGCGGCAACTGCAGCACCGCCGCCGGCGACGCTCCCGATCCCCGCCCCTCCAACGGCCACGTCCAAGTCCTCCCCCCAAATGCCGCCCCGACCCGACCCGCCCCCCCGCCCAAACCCCACACCACCGCCCCAACTACCACCACCGGCCGCGTCCTCGGCAAGCCCATGGAGGACGTCCGCTCCACCTACACCTTCGGCCGCGAGCTCGGCCGCGGCCAATTCGGCGTCACCTACCTCGTGACTCACAAAGAGACCAAGCAGCAGTTCGCCTGCAAATCCATCGCTACTCGGAAGCTGATCAACCACGACGACGTGGAGGACGTCCGCCGTGAGGTCCAGATCATGCACCACCTCACCGGCCACCGCAACATCGTCGAGCTCAAAGGCGCGTACGAGGACCGCCACTCGGTCAACCTGGTCATGGAGCTCTGCGCCGGCGGCGAGCTTTTCGATAGGATCATCGCCAAGGGCCACTATTCGGAGAGGGCGGCGGCGAATCTCTGCCGTCAGATGGTGACCGTCGTTCATTACTGTCACTCTATGGGGGTGTTTCACAGGGACTTGAAGCCGGAGAATTTCTTGCTGTTGAGTAAGGATGAGGACTCGCCGCTCAAGGCCACCGATTTCGGACTCTCCGTCTTCTTCAAGCCCGAGAATGAACAGGGTATCTTTGATGCTATTCTTCGGGGTCACATTGATTTCTCATCTGATCCTTGGCCTTCCATATCCAGCAGTGCCAAAGATCTTGTGAAGAAAATGCTTCGAGCTGATCCAAAGGAACGGCTTGCAGCAGTGGATGTCCTTAATCATCCATGGATGCGAGAAGATGGCGATGCATCTGATAAACCTCTTGATATTGCTGTTTTATCTAGAATGAAACAGTTCAGGGCAATGAACAAACTCAAAAAAGTTGCATTGAAGGTAATTGCAGAAAATCTTTCTGAAGAGGAAATCATAGGCTTGAAGGAAATGTTTAAATCAATGGACACAGATAACAGTGGAACAATTACATTTGAAGAGCTAAAAGCGGGCCTTCCAAAGCTGGGTACCAAGCTTTCTGAGTCTGAAGTGAGACAGTTGATGGAAGCGGCTGATGTTGATGGAAATGGAACAATTGATTACATTGAGTTTATAACCGCTACAATGCACATGAACAGAATGGATAGAGAGGACCATTTATACACAGCCTTTGAATATTTTGACAAGGACAAGAGCGGGTACATCACGATGGAAGAATTGGAGCAAGCACTTAAGAAGTATAATATGGGTGATGAACAAACGATTAAGGATATCATCGCAGAAGTTGACACAGACCTTGATGGAAGGATCAACTATGATGAGTTTGTTGCCATGATGAGGAAAGGAAATCCTGAGTTGGTCACAAACAGAAGAAGAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010119 GO:0010359 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

467

Amino Acids

52.6

Weight (kDa)

5.93

Isoelectric Point (pI)

35.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	64 - 220	1.5e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	65 - 217	3.1e-21	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	227 - 274	8.6e-08	Protein kinase domain
EF-hand_1	PF00036	322 - 348	6.6e-08	EF hand domain
EF-hand_7	PF13499	322 - 382	2.7e-13	EF-hand domain pair
EF-hand_6	PF13405	322 - 350	1.7e-06	EF-hand domain
EF-hand_8	PF13833	334 - 380	1.6e-07	EF-hand domain pair
EF-hand_7	PF13499	391 - 454	1.8e-16	EF-hand domain pair
EF-hand_6	PF13405	394 - 421	5.5e-07	EF-hand domain
EF-hand_1	PF00036	396 - 420	2.2e-06	EF hand domain
EF-hand_8	PF13833	406 - 455	3.4e-08	EF-hand domain pair
EF-hand_1	PF00036	429 - 455	7.3e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014884)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23650
fragaria_vesca	FvH4_5g03610 FvH4_5g03610 FvH4_5g03610
malus_domestica	MD06G1095900.v1.1 MD14G1116100.v1.1
prunus_persica	Prupe.5G110500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0196111
rosa_laevigata	RLG00000004064
rosa_multiflora	Rmu_sc0013391.1_g000002
rosa_roxburghii	Rroxscaffold_3G00259800
rosa_rugosa	Rorug07G0030700
rosa_samantha	Rh7AG156500 Rh7BG158100 Rh7CG162000
rosa_wichuraiana	Rw7G013370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1136
AatII	GACGTC	2 cut(s)	180, 327
Acc65I	GGTACC	1 cut(s)	1049
AccB1I	GGYRCC	1 cut(s)	1049
AccBSI	CCGCTC	3 cut(s)	183, 613, 1218
AccII	CGCG	4 cut(s)	153, 204, 216, 392
AclWI	GGATC	5 cut(s)	35, 467, 707, 767, 1337
AcoI	YGGCCR	6 cut(s)	58, 148, 199, 211, 217, 361
AcsI	RAATTY	1 cut(s)	577
AcuI	CTGAAG	2 cut(s)	966, 1089
AcyI	GRCGYC	3 cut(s)	177, 228, 324
AfaI	GTAC	3 cut(s)	395, 1051, 1223
AfiI	CCNNNNNNNGG	8 cut(s)	56, 334, 409, 424, 481, 514, 1046, 1359
AflII	CTTAAG	1 cut(s)	1253
AgsI	TTSAA	6 cut(s)	568, 649, 922, 967, 1019, 1196
AhdI	GACNNNNNGTC	1 cut(s)	638
AjiI	CACGTC	2 cut(s)	64, 316
AjnI	CCWGG	1 cut(s)	417
AjuI	GAANNNNNNNTTGG	2 cut(s)	251, 283
AluBI	AGCT	9 cut(s)	208, 298, 382, 430, 448, 770, 1024, 1045, 1057
AluI	AGCT	9 cut(s)	208, 298, 382, 430, 448, 770, 1024, 1045, 1057
Alw21I	GWGCWC	3 cut(s)	210, 384, 432
Alw26I	GTCTC	2 cut(s)	248, 1069
AlwI	GGATC	5 cut(s)	35, 467, 707, 767, 1337
Ama87I	CYCGRG	1 cut(s)	653
ApeKI	GCWGC	3 cut(s)	15, 262, 791
ApoI	RAATTY	1 cut(s)	577
Asp700I	GAANNNNTTC	1 cut(s)	884
Asp718I	GGTACC	1 cut(s)	1049
AspLEI	GCGC	2 cut(s)	392, 437
AspS9I	GGNCC	5 cut(s)	337, 400, 472, 1033, 1174
AsuHPI	GGTGA	4 cut(s)	223, 349, 529, 1283
AvaI	CYCGRG	1 cut(s)	653
AvaII	GGWCC	3 cut(s)	337, 400, 1174
BanI	GGYRCC	1 cut(s)	1049
BanII	GRGCYC	3 cut(s)	210, 384, 432
BauI	CACGAG	1 cut(s)	239
BbsI	GAAGAC	1 cut(s)	634
Bbv12I	GWGCWC	3 cut(s)	210, 384, 432
BbvI	GCAGC	3 cut(s)	27, 274, 803
BccI	CCATC	7 cut(s)	290, 508, 824, 1078, 1097, 1225, 1316
BceAI	ACGGC	4 cut(s)	73, 315, 492, 800
BcgI	CGANNNNNNTGC	4 cut(s)	265, 299, 358, 392
BciT130I	CCWGG	1 cut(s)	419
BclI	TGATCA	1 cut(s)	300
BcoDI	GTCTC	2 cut(s)	248, 1069
BfaI	CTAG	1 cut(s)	873
BfmI	CTRYAG	1 cut(s)	13
BfrI	CTTAAG	1 cut(s)	1253
BglI	GCCNNNNNGGC	1 cut(s)	225
BglII	AGATCT	1 cut(s)	745
Bme1390I	CCNGG	1 cut(s)	419
Bme18I	GGWCC	3 cut(s)	337, 400, 1174
BmeRI	GACNNNNNGTC	1 cut(s)	638
BmeT110I	CYCGRG	1 cut(s)	653
BmgBI	CACGTC	2 cut(s)	64, 316
BmgT120I	GGNCC	5 cut(s)	337, 400, 472, 1033, 1174
BmiI	GGNNCC	1 cut(s)	1051
BmrFI	CCNGG	1 cut(s)	419
BmsI	GCATC	4 cut(s)	667, 810, 826, 848
BpiI	GAAGAC	1 cut(s)	634
BpuEI	CTTGAG	1 cut(s)	599
BsaHI	GRCGYC	3 cut(s)	177, 228, 324
BsaI	GGTCTC	1 cut(s)	248
BsaJI	CCNNGG	6 cut(s)	157, 168, 214, 468, 716, 815
BsaXI	ACNNNNNCTCC	2 cut(s)	167, 197
Bsc4I	CCNNNNNNNGG	8 cut(s)	56, 334, 409, 424, 481, 514, 1046, 1359
Bse118I	RCCGGY	4 cut(s)	26, 146, 359, 437
Bse3DI	GCAATG	1 cut(s)	900
BseBI	CCWGG	1 cut(s)	419
BseDI	CCNNGG	6 cut(s)	157, 168, 214, 468, 716, 815
BseGI	GGATG	4 cut(s)	604, 805, 811, 825
BseLI	CCNNNNNNNGG	8 cut(s)	56, 334, 409, 424, 481, 514, 1046, 1359
BseMI	GCAATG	1 cut(s)	900
BseMII	CTCAG	2 cut(s)	1053, 1365
BseX3I	CGGCCG	3 cut(s)	148, 199, 211
BseXI	GCAGC	3 cut(s)	27, 274, 803
BseYI	CCCAGC	1 cut(s)	1045
Bsh1236I	CGCG	4 cut(s)	153, 204, 216, 392
Bsh1285I	CGRYCG	3 cut(s)	151, 202, 214
BshNI	GGYRCC	1 cut(s)	1049
BsiEI	CGRYCG	3 cut(s)	151, 202, 214
BsiHKAI	GWGCWC	3 cut(s)	210, 384, 432
BsiHKCI	CYCGRG	1 cut(s)	653
BsiSI	CCGG	5 cut(s)	27, 147, 360, 438, 572
BsiWI	CGTACG	1 cut(s)	393
BslFI	GGGAC	1 cut(s)	575
BslI	CCNNNNNNNGG	8 cut(s)	56, 334, 409, 424, 481, 514, 1046, 1359
BsmAI	GTCTC	2 cut(s)	248, 1069
BsmFI	GGGAC	1 cut(s)	575
Bso31I	GGTCTC	1 cut(s)	248
BsoBI	CYCGRG	1 cut(s)	653
Bsp1286I	GDGCHC	3 cut(s)	210, 384, 432
Bsp143I	GATC	8 cut(s)	40, 300, 342, 459, 712, 745, 772, 1329
Bsp19I	CCATGG	2 cut(s)	168, 815
BspCNI	CTCAG	2 cut(s)	1054, 1366
BspFNI	CGCG	4 cut(s)	153, 204, 216, 392
BspLI	GGNNCC	1 cut(s)	1051
BspMAI	CTGCAG	1 cut(s)	17
BspPI	GGATC	5 cut(s)	35, 467, 707, 767, 1337
BspQI	GCTCTTC	1 cut(s)	1014
BspT107I	GGYRCC	1 cut(s)	1049
BspTI	CTTAAG	1 cut(s)	1253
BspTNI	GGTCTC	1 cut(s)	248
BsrBI	CCGCTC	3 cut(s)	183, 613, 1218
BsrDI	GCAATG	1 cut(s)	900
BsrFI	RCCGGY	4 cut(s)	26, 146, 359, 437
BssAI	RCCGGY	4 cut(s)	26, 146, 359, 437
BssECI	CCNNGG	6 cut(s)	157, 168, 214, 468, 716, 815
BssMI	GATC	8 cut(s)	40, 300, 342, 459, 712, 745, 772, 1329
BssNI	GRCGYC	3 cut(s)	177, 228, 324
BssSI	CACGAG	1 cut(s)	239
BssT1I	CCWWGG	4 cut(s)	168, 468, 716, 815
Bst2BI	CACGAG	1 cut(s)	239
Bst2UI	CCWGG	1 cut(s)	419
Bst4CI	ACNGT	5 cut(s)	523, 536, 885, 1001, 1080
Bst6I	CTCTTC	2 cut(s)	942, 1014
BstACI	GRCGYC	3 cut(s)	177, 228, 324
BstAFI	CTTAAG	1 cut(s)	1253
BstC8I	GCNNGC	9 cut(s)	28, 164, 206, 274, 439, 446, 789, 1033, 1248
BstDEI	CTNAG	2 cut(s)	1062, 1374
BstDSI	CCRYGG	3 cut(s)	168, 214, 815
BstEII	GGTNACC	1 cut(s)	517
BstF5I	GGATG	4 cut(s)	604, 805, 811, 825
BstFNI	CGCG	4 cut(s)	153, 204, 216, 392
BstHHI	GCGC	2 cut(s)	392, 437
BstKTI	GATC	8 cut(s)	43, 303, 345, 462, 715, 748, 775, 1332
BstMAI	GTCTC	2 cut(s)	248, 1069
BstMBI	GATC	8 cut(s)	40, 300, 342, 459, 712, 745, 772, 1329
BstMCI	CGRYCG	3 cut(s)	151, 202, 214
BstMWI	GCNNNNNNNGC	7 cut(s)	12, 15, 32, 159, 210, 225, 441
BstNI	CCWGG	1 cut(s)	419
BstPI	GGTNACC	1 cut(s)	517
BstSCI	CCNGG	1 cut(s)	417
BstSFI	CTRYAG	1 cut(s)	13
BstUI	CGCG	4 cut(s)	153, 204, 216, 392
BstV1I	GCAGC	3 cut(s)	27, 274, 803
BstV2I	GAAGAC	1 cut(s)	634
BstX2I	RGATCY	1 cut(s)	745
BstYI	RGATCY	1 cut(s)	745
BstZI	CGGCCG	3 cut(s)	148, 199, 211
BtgI	CCRYGG	3 cut(s)	168, 214, 815
BtgZI	GCGATG	4 cut(s)	268, 448, 849, 1282
BtrI	CACGTC	2 cut(s)	64, 316
BtsCI	GGATG	4 cut(s)	604, 805, 811, 825
BtsI	GCAGTG	2 cut(s)	742, 801
BtsIMutI	CAGTG	3 cut(s)	742, 801, 1006
Cac8I	GCNNGC	9 cut(s)	28, 164, 206, 274, 439, 446, 789, 1033, 1248
CfoI	GCGC	2 cut(s)	392, 437
Cfr10I	RCCGGY	4 cut(s)	26, 146, 359, 437
Cfr13I	GGNCC	5 cut(s)	337, 400, 472, 1033, 1174
Cfr42I	CCGCGG	1 cut(s)	217
CseI	GACGC	3 cut(s)	41, 142, 217
CsiI	ACCWGGT	1 cut(s)	417
Csp6I	GTAC	3 cut(s)	394, 1050, 1222
CviAII	CATG	6 cut(s)	169, 346, 424, 816, 1153, 1354
CviQI	GTAC	3 cut(s)	394, 1050, 1222
DdeI	CTNAG	2 cut(s)	1062, 1374
DpnI	GATC	8 cut(s)	42, 302, 344, 461, 714, 747, 774, 1331
DpnII	GATC	8 cut(s)	40, 300, 342, 459, 712, 745, 772, 1329
DraI	TTTAAA	1 cut(s)	979
DriI	GACNNNNNGTC	1 cut(s)	638
EaeI	YGGCCR	6 cut(s)	58, 148, 199, 211, 217, 361
EagI	CGGCCG	3 cut(s)	148, 199, 211
Eam1104I	CTCTTC	2 cut(s)	942, 1014
Eam1105I	GACNNNNNGTC	1 cut(s)	638
EarI	CTCTTC	2 cut(s)	942, 1014
EciI	GGCGGA	1 cut(s)	317
Ecl136II	GAGCTC	3 cut(s)	208, 382, 430
EclXI	CGGCCG	3 cut(s)	148, 199, 211
Eco130I	CCWWGG	4 cut(s)	168, 468, 716, 815
Eco24I	GRGCYC	3 cut(s)	210, 384, 432
Eco31I	GGTCTC	1 cut(s)	248
Eco32I	GATATC	1 cut(s)	1294
Eco47I	GGWCC	3 cut(s)	337, 400, 1174
Eco52I	CGGCCG	3 cut(s)	148, 199, 211
Eco53kI	GAGCTC	3 cut(s)	208, 382, 430
Eco57I	CTGAAG	2 cut(s)	966, 1089
Eco88I	CYCGRG	1 cut(s)	653
Eco91I	GGTNACC	1 cut(s)	517
EcoICRI	GAGCTC	3 cut(s)	208, 382, 430
EcoO65I	GGTNACC	1 cut(s)	517
EcoRII	CCWGG	1 cut(s)	417
EcoRV	GATATC	1 cut(s)	1294
EcoT14I	CCWWGG	4 cut(s)	168, 468, 716, 815
EcoT22I	ATGCAT	1 cut(s)	841
EcoT38I	GRGCYC	3 cut(s)	210, 384, 432
ErhI	CCWWGG	4 cut(s)	168, 468, 716, 815
FaeI	CATG	6 cut(s)	172, 349, 427, 819, 1156, 1357
FalI	AAGNNNNNCTT	2 cut(s)	1020, 1052
FaqI	GGGAC	1 cut(s)	575
FatI	CATG	6 cut(s)	168, 345, 423, 815, 1152, 1353
FauI	CCCGC	5 cut(s)	53, 109, 117, 1024, 1211
FbaI	TGATCA	1 cut(s)	300
FokI	GGATG	4 cut(s)	611, 798, 812, 832
FriOI	GRGCYC	3 cut(s)	210, 384, 432
FspBI	CTAG	1 cut(s)	873
GlaI	GCGC	2 cut(s)	391, 436
GsaI	CCCAGC	1 cut(s)	1049
HapII	CCGG	5 cut(s)	27, 147, 360, 438, 572
HgaI	GACGC	3 cut(s)	41, 142, 217
HhaI	GCGC	2 cut(s)	392, 437
Hin1I	GRCGYC	3 cut(s)	177, 228, 324
Hin1II	CATG	6 cut(s)	172, 349, 427, 819, 1156, 1357
Hin6I	GCGC	2 cut(s)	390, 435
HinP1I	GCGC	2 cut(s)	390, 435
HincII	GTYRAC	2 cut(s)	415, 1309
HindII	GTYRAC	2 cut(s)	415, 1309
HindIII	AAGCTT	1 cut(s)	1055
HinfI	GANTC	5 cut(s)	244, 499, 605, 633, 1064
HpaII	CCGG	5 cut(s)	27, 147, 360, 438, 572
HphI	GGTGA	4 cut(s)	223, 349, 529, 1283
Hpy166II	GTNNAC	2 cut(s)	415, 1309
Hpy188I	TCNGA	9 cut(s)	294, 484, 513, 632, 712, 844, 946, 1063, 1069
Hpy188III	TCNNGA	7 cut(s)	38, 241, 340, 854, 873, 1228, 1373
Hpy8I	GTNNAC	2 cut(s)	415, 1309
Hpy99I	CGWCG	5 cut(s)	35, 314, 317, 380, 527
HpyAV	CCTTC	6 cut(s)	205, 732, 916, 961, 1046, 1320
HpyCH4III	ACNGT	5 cut(s)	523, 536, 885, 1001, 1080
HpyCH4IV	ACGT	4 cut(s)	63, 177, 315, 324
HpyCH4V	TGCA	8 cut(s)	15, 276, 349, 791, 839, 917, 932, 1150
HpyF10VI	GCNNNNNNNGC	7 cut(s)	12, 15, 32, 159, 210, 225, 441
HpyF3I	CTNAG	2 cut(s)	1062, 1374
HpySE526I	ACGT	4 cut(s)	63, 177, 315, 324
Hsp92I	GRCGYC	3 cut(s)	177, 228, 324
Hsp92II	CATG	6 cut(s)	172, 349, 427, 819, 1156, 1357
HspAI	GCGC	2 cut(s)	390, 435
KpnI	GGTACC	1 cut(s)	1053
KroI	GCCGGC	2 cut(s)	26, 437
KroNI	GCCGGC	2 cut(s)	28, 439
Ksp22I	TGATCA	1 cut(s)	300
KspI	CCGCGG	1 cut(s)	217
Kzo9I	GATC	8 cut(s)	40, 300, 342, 459, 712, 745, 772, 1329
LguI	GCTCTTC	1 cut(s)	1014
LmnI	GCTCC	4 cut(s)	40, 188, 427, 1243
Lsp1109I	GCAGC	3 cut(s)	27, 274, 803
LweI	GCATC	4 cut(s)	667, 810, 826, 848
MabI	ACCWGGT	1 cut(s)	417
MaeI	CTAG	1 cut(s)	873
MaeII	ACGT	4 cut(s)	63, 177, 315, 324
MaeIII	GTNAC	6 cut(s)	229, 241, 517, 536, 692, 1381
MalI	GATC	8 cut(s)	42, 302, 344, 461, 714, 747, 774, 1331
MbiI	CCGCTC	3 cut(s)	183, 613, 1218
MboI	GATC	8 cut(s)	40, 300, 342, 459, 712, 745, 772, 1329
MboII	GAAGA	9 cut(s)	634, 637, 677, 766, 839, 959, 1031, 1247, 1404
MfeI	CAATTG	1 cut(s)	1116
MflI	RGATCY	1 cut(s)	745
MhlI	GDGCHC	3 cut(s)	210, 384, 432
MluCI	AATT	6 cut(s)	221, 577, 927, 1008, 1116, 1238
MlyI	GAGTC	4 cut(s)	238, 599, 627, 1073
MmeI	TCCRAC	1 cut(s)	78
Mph1103I	ATGCAT	1 cut(s)	841
MreI	CGCCGGCG	2 cut(s)	26, 437
MroNI	GCCGGC	2 cut(s)	26, 437
MroXI	GAANNNNTTC	1 cut(s)	884
MseI	TTAA	4 cut(s)	807, 978, 1254, 1287
MslI	CAYNNNNRTG	1 cut(s)	1229
MspA1I	CMGCKG	1 cut(s)	216
MspCI	CTTAAG	1 cut(s)	1253
MspI	CCGG	5 cut(s)	27, 147, 360, 438, 572
MspR9I	CCNGG	1 cut(s)	419
MunI	CAATTG	1 cut(s)	1116
MvaI	CCWGG	1 cut(s)	419
MvnI	CGCG	4 cut(s)	153, 204, 216, 392
MwoI	GCNNNNNNNGC	7 cut(s)	12, 15, 32, 159, 210, 225, 441
NaeI	GCCGGC	2 cut(s)	28, 439
NcoI	CCATGG	2 cut(s)	168, 815
NdeII	GATC	8 cut(s)	40, 300, 342, 459, 712, 745, 772, 1329
NgoMIV	GCCGGC	2 cut(s)	26, 437
NlaIII	CATG	6 cut(s)	172, 349, 427, 819, 1156, 1357
NlaIV	GGNNCC	1 cut(s)	1051
NmeAIII	GCCGAG	2 cut(s)	138, 189
NmuCI	GTSAC	6 cut(s)	229, 241, 517, 536, 692, 1381
NsiI	ATGCAT	1 cut(s)	841
PciSI	GCTCTTC	1 cut(s)	1014
PcsI	WCGNNNNNNNCGW	2 cut(s)	321, 636
PdiI	GCCGGC	2 cut(s)	28, 439
PdmI	GAANNNNTTC	1 cut(s)	884
PfeI	GAWTC	1 cut(s)	499
Pfl23II	CGTACG	1 cut(s)	393
PleI	GAGTC	4 cut(s)	238, 599, 627, 1072
PpsI	GAGTC	4 cut(s)	238, 599, 627, 1072
PsiI	TTATAA	1 cut(s)	1136
Psp124BI	GAGCTC	3 cut(s)	210, 384, 432
Psp6I	CCWGG	1 cut(s)	417
PspEI	GGTNACC	1 cut(s)	517
PspFI	CCCAGC	1 cut(s)	1045
PspGI	CCWGG	1 cut(s)	417
PspLI	CGTACG	1 cut(s)	393
PspN4I	GGNNCC	1 cut(s)	1051
PspPI	GGNCC	5 cut(s)	337, 400, 472, 1033, 1174
PstI	CTGCAG	1 cut(s)	17
PsuI	RGATCY	1 cut(s)	745
RsaI	GTAC	3 cut(s)	395, 1051, 1223
RsaNI	GTAC	3 cut(s)	394, 1050, 1222
RseI	CAYNNNNRTG	1 cut(s)	1229
SacI	GAGCTC	3 cut(s)	210, 384, 432
SacII	CCGCGG	1 cut(s)	217
SapI	GCTCTTC	1 cut(s)	1014
SaqAI	TTAA	4 cut(s)	807, 978, 1254, 1287
Sau3AI	GATC	8 cut(s)	40, 300, 342, 459, 712, 745, 772, 1329
Sau96I	GGNCC	5 cut(s)	337, 400, 472, 1033, 1174
SchI	GAGTC	4 cut(s)	238, 599, 627, 1073
ScrFI	CCNGG	1 cut(s)	419
SduI	GDGCHC	3 cut(s)	210, 384, 432
SexAI	ACCWGGT	1 cut(s)	417
SfaNI	GCATC	4 cut(s)	667, 810, 826, 848
SfcI	CTRYAG	1 cut(s)	13
Sfr303I	CCGCGG	1 cut(s)	217
SgrAI	CRCCGGYG	2 cut(s)	26, 437
SgrBI	CCGCGG	1 cut(s)	217
SinI	GGWCC	3 cut(s)	337, 400, 1174
SmiMI	CAYNNNNRTG	1 cut(s)	1229
SmlI	CTYRAG	2 cut(s)	614, 1253
SmoI	CTYRAG	2 cut(s)	614, 1253
Sse9I	AATT	6 cut(s)	221, 577, 927, 1008, 1116, 1238
SspI	AATATT	1 cut(s)	1199
SspMI	CTAG	1 cut(s)	873
SstI	GAGCTC	3 cut(s)	210, 384, 432
StyD4I	CCNGG	1 cut(s)	417
StyI	CCWWGG	4 cut(s)	168, 468, 716, 815
TaaI	ACNGT	5 cut(s)	523, 536, 885, 1001, 1080
TaiI	ACGT	4 cut(s)	66, 180, 318, 327
TaqI	TCGA	3 cut(s)	378, 453, 766
TaqII	GACCGA	1 cut(s)	400
TasI	AATT	6 cut(s)	221, 577, 927, 1008, 1116, 1238
TfiI	GAWTC	1 cut(s)	499
Tru1I	TTAA	4 cut(s)	807, 978, 1254, 1287
Tru9I	TTAA	4 cut(s)	807, 978, 1254, 1287
TscAI	CASTG	3 cut(s)	742, 801, 1006
TseFI	GTSAC	6 cut(s)	229, 241, 517, 536, 692, 1381
TseI	GCWGC	3 cut(s)	15, 262, 791
Tsp45I	GTSAC	6 cut(s)	229, 241, 517, 536, 692, 1381
TspDTI	ATGAA	6 cut(s)	518, 675, 893, 911, 1169, 1290
TspGWI	ACGGA	1 cut(s)	628
TspRI	CASTG	3 cut(s)	742, 801, 1006
Vha464I	CTTAAG	1 cut(s)	1253
VpaK11BI	GGWCC	3 cut(s)	337, 400, 1174
XapI	RAATTY	1 cut(s)	577
XbaI	TCTAGA	1 cut(s)	872
XmnI	GAANNNNTTC	1 cut(s)	884
XspI	CTAG	1 cut(s)	873
ZraI	GACGTC	2 cut(s)	178, 325
Zsp2I	ATGCAT	1 cut(s)	841

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.