MD06G1102600.v1.1

Probable zinc-ribbon domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008950
Physical Location & Seq
Forward (+)
23973440 .. 23975193
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1102600.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGATTCCATGTTTTCATCTTTTTGTCTACTTCTTGTTGGTGATGGATTTGAAGGGAGGTAGAGGAAAAGAGAGAGTTTTCGGAGAAAGAAACGGTGAGTCGATTAGTGGTAGTTCATCAAGAACAGACAGCAAAAAGGTTATGGTTGATAATTATGGTAATAATGTTAGGGAAGGGGTTAAGGGTTTAAGATTTGAGCAAAGAGGTGCAGCTATAGAAACCGAGTTTGTTGAAAAACATACTCGAATTTCGCAGTTCCCGACTGATAATTGGATCTCTGGAGATGAGATTGAAATGAACGTGAGTAGGTGTACGTCTGTAGTTTCAAGTGTAGAAGATATTTCTGCCCACTTAAAGAATTTTGCTGGGTCGGTGAGATCAAGGGAAAGTATGGACCAGTTTCGAGGTGTAGAAAGAGATCGGTTTTATGGGTTTTACGCGAGCCCTACGGTCGGGGCTCGGCGTGGAAGAATGCCAACTTCTGCTTATCCTGACGAGGGGCCTTCAACCTACAAGGTAGACTCTTTTGATGGTACGAGTGAAGTTGAAAATTTAGAGCAGGATCGAGCGGAGCTTCTAAAGAAGTTGGATGAGCTGAAGGAGAAACTGAGCAGATATTATGATGTGGCAGATAAACCAAGGGAACCGGTTCCTACGGAGAGGAGTAGAACGCTGCCTGATCCTTATGGTAACCTGTTAACTTATACTCTTTCAATGCAGCCTTGCGCTGTGGACGAGCAGATGACAAGACCCCCTTACTTCAACCATAGCCATGGACCTGTAACTTTTACGGAGCATCATAATATGGTTATGCGGCAGAAGAACTTCGATCCTCCTCAAAGGCATCCATGGAATGCGATTCCAGAAAATGAGGATCCGTCTCAGCAACAAATGAAACGGAGGCCTCCCCATCATCCACTCCAATACCCAAGACCACCACTAAACGAAAAATTTATGGGACAGCGCATGGAATTCAATGCAAATCCACATGAAAAAGCTTCTCACTCACCCGCTTGCTCTTGTTCAGGCTGCTACAACCCAAATTGGGTAGTTCCACCCCAAGTCCCTCTTGATGATTTTGGAAATCAAAGGGTTCCAAAAGCTCCAATTAATTTGAATTCCAACCACCATGTCAATCCTGTTATATTTCTGCCACATGATTATAATCCACGACATGCTAGTCTTCCTCCGTTGCACACAAGGTGGCAAAGCGACTTTGATTCAGAACCAAGAACCGCTATGGCAATCAATAGGCGTGCACAAATCTTCCATCCTGTAGCAGGTGGTGCCCCATTCATCACATGCTTTAGGTGCTTTGAAATACTGAAACTTCCGAGAAAACTTGAGAATATGAATAAGAATCAGTCAAAACTGCAGTGTGGTTCCTGTTCAACTGTTATCTCACTTGAAATCCAGAACAAAAAGCTCTTTACTTCTAAAGATTCCAAGCGACAATCTTCTGAGTTTGGTCAAAGTTCTAATGAGGCATTAAAAGGCAGCGTTTTAAGTCCTCGTGGTAGTCCGAATGAAGAAGCTGGACGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zinc_ribbon_12

Protein Analysis

516

Amino Acids

58.56

Weight (kDa)

8.56

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_12 PF11331 427 - 471 2.6e-20 EDR4 zinc-ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1164
AarI CACCTGC 1 cut(s) 1271
AasI GACNNNNNNGTC 1 cut(s) 1179
Acc36I ACCTGC 1 cut(s) 1271
AccB1I GGYRCC 1 cut(s) 1286
AccBSI CCGCTC 1 cut(s) 569
AccI GTMKAC 2 cut(s) 27, 519
AccII CGCG 1 cut(s) 440
AciI CCGC 4 cut(s) 569, 814, 1011, 1236
AclWI GGATC 6 cut(s) 281, 570, 674, 824, 869, 882
AcsI RAATTY 6 cut(s) 246, 358, 550, 950, 971, 1117
AcuI CTGAAG 1 cut(s) 617
AdeI CACNNNGTG 1 cut(s) 1203
AfaI GTAC 2 cut(s) 313, 535
AfiI CCNNNNNNNGG 4 cut(s) 452, 497, 1045, 1280
AgeI ACCGGT 1 cut(s) 646
AjiI CACGTC 1 cut(s) 1541
AluBI AGCT 7 cut(s) 212, 574, 595, 998, 1103, 1426, 1535
AluI AGCT 7 cut(s) 212, 574, 595, 998, 1103, 1426, 1535
Alw21I GWGCWC 1 cut(s) 1261
Alw26I GTCTC 1 cut(s) 885
Alw44I GTGCAC 1 cut(s) 1257
AlwI GGATC 6 cut(s) 281, 570, 674, 824, 869, 882
AoxI GGCC 2 cut(s) 500, 902
ApaLI GTGCAC 1 cut(s) 1257
ApeKI GCWGC 5 cut(s) 209, 673, 718, 1029, 1497
ApoI RAATTY 6 cut(s) 246, 358, 550, 950, 971, 1117
AseI ATTAAT 1 cut(s) 1110
AsiGI ACCGGT 1 cut(s) 646
Asp700I GAANNNNTTC 2 cut(s) 648, 824
AspLEI GCGC 2 cut(s) 728, 966
AspS9I GGNCC 3 cut(s) 394, 500, 776
AsuHPI GGTGA 4 cut(s) 52, 107, 385, 999
AvaII GGWCC 2 cut(s) 394, 776
BaeGI GKGCMC 2 cut(s) 1261, 1291
BamHI GGATCC 1 cut(s) 874
BanI GGYRCC 1 cut(s) 1286
BanII GRGCYC 2 cut(s) 446, 460
BauI CACGAG 1 cut(s) 1512
BbsI GAAGAC 1 cut(s) 1175
Bbv12I GWGCWC 1 cut(s) 1261
BbvI GCAGC 5 cut(s) 221, 660, 730, 1016, 1509
BccI CCATC 4 cut(s) 37, 524, 918, 1278
BcoDI GTCTC 1 cut(s) 885
BfaI CTAG 1 cut(s) 1179
BfmI CTRYAG 4 cut(s) 213, 318, 1275, 1373
BfuAI ACCTGC 1 cut(s) 1271
BisI GCNGC 6 cut(s) 210, 674, 719, 815, 1030, 1498
BlsI GCNGC 6 cut(s) 211, 675, 720, 816, 1031, 1499
Bme18I GGWCC 2 cut(s) 394, 776
BmgBI CACGTC 1 cut(s) 1541
BmgT120I GGNCC 3 cut(s) 394, 500, 776
BmiI GGNNCC 7 cut(s) 501, 645, 651, 876, 1095, 1288, 1384
BmsI GCATC 2 cut(s) 805, 853
BpiI GAAGAC 1 cut(s) 1175
BpmI CTGGAG 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 1364
BsaBI GATNNNNATC 1 cut(s) 1164
BsaJI CCNNGG 3 cut(s) 638, 772, 848
BsaWI WCCGGW 1 cut(s) 646
Bsc4I CCNNNNNNNGG 4 cut(s) 452, 497, 1045, 1280
Bse118I RCCGGY 1 cut(s) 646
Bse1I ACTGG 1 cut(s) 397
Bse8I GATNNNNATC 1 cut(s) 1164
BseDI CCNNGG 3 cut(s) 638, 772, 848
BseGI GGATG 4 cut(s) 595, 844, 913, 1270
BseJI GATNNNNATC 1 cut(s) 1164
BseLI CCNNNNNNNGG 4 cut(s) 452, 497, 1045, 1280
BseMII CTCAG 3 cut(s) 599, 896, 1452
BseNI ACTGG 1 cut(s) 397
BseRI GAGGAG 2 cut(s) 676, 825
BseSI GKGCMC 2 cut(s) 1261, 1291
BseXI GCAGC 5 cut(s) 221, 660, 730, 1016, 1509
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 228
Bsh1236I CGCG 1 cut(s) 440
Bsh1285I CGRYCG 1 cut(s) 453
BshFI GGCC 2 cut(s) 502, 904
BshNI GGYRCC 1 cut(s) 1286
BshTI ACCGGT 1 cut(s) 646
BsiEI CGRYCG 1 cut(s) 453
BsiHKAI GWGCWC 1 cut(s) 1261
BsiSI CCGG 1 cut(s) 647
BslFI GGGAC 2 cut(s) 972, 1049
BslI CCNNNNNNNGG 4 cut(s) 452, 497, 1045, 1280
BsmAI GTCTC 1 cut(s) 885
BsmBI CGTCTC 1 cut(s) 885
BsmFI GGGAC 2 cut(s) 972, 1049
BsmI GAATGC 2 cut(s) 477, 859
BsnI GGCC 2 cut(s) 502, 904
Bsp1286I GDGCHC 4 cut(s) 446, 460, 1261, 1291
Bsp143I GATC 7 cut(s) 273, 377, 418, 562, 679, 829, 874
Bsp19I CCATGG 2 cut(s) 772, 848
BspACI CCGC 4 cut(s) 569, 814, 1011, 1236
BspANI GGCC 2 cut(s) 502, 904
BspCNI CTCAG 3 cut(s) 600, 895, 1453
BspFNI CGCG 1 cut(s) 440
BspLI GGNNCC 7 cut(s) 501, 645, 651, 876, 1095, 1288, 1384
BspMAI CTGCAG 1 cut(s) 1377
BspMI ACCTGC 1 cut(s) 1271
BspPI GGATC 6 cut(s) 281, 570, 674, 824, 869, 882
BspT107I GGYRCC 1 cut(s) 1286
BsrBI CCGCTC 1 cut(s) 569
BsrFI RCCGGY 1 cut(s) 646
BsrI ACTGG 1 cut(s) 397
BssAI RCCGGY 1 cut(s) 646
BssECI CCNNGG 3 cut(s) 638, 772, 848
BssMI GATC 7 cut(s) 273, 377, 418, 562, 679, 829, 874
BssSI CACGAG 1 cut(s) 1512
BssT1I CCWWGG 3 cut(s) 638, 772, 848
Bst2BI CACGAG 1 cut(s) 1512
Bst4CI ACNGT 3 cut(s) 95, 451, 1396
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstC8I GCNNGC 3 cut(s) 442, 1015, 1257
BstDEI CTNAG 3 cut(s) 608, 882, 1461
BstDSI CCRYGG 2 cut(s) 772, 848
BstEII GGTNACC 1 cut(s) 689
BstENI CCTNNNNNAGG 1 cut(s) 1278
BstF5I GGATG 4 cut(s) 595, 844, 913, 1270
BstFNI CGCG 1 cut(s) 440
BstHHI GCGC 2 cut(s) 728, 966
BstKTI GATC 7 cut(s) 276, 380, 421, 565, 682, 832, 877
BstMAI GTCTC 1 cut(s) 885
BstMBI GATC 7 cut(s) 273, 377, 418, 562, 679, 829, 874
BstMCI CGRYCG 1 cut(s) 453
BstMWI GCNNNNNNNGC 2 cut(s) 1286, 1311
BstNSI RCATGY 2 cut(s) 1178, 1305
BstPI GGTNACC 1 cut(s) 689
BstSFI CTRYAG 4 cut(s) 213, 318, 1275, 1373
BstSLI GKGCMC 2 cut(s) 1261, 1291
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 5 cut(s) 221, 660, 730, 1016, 1509
BstV2I GAAGAC 1 cut(s) 1175
BstX2I RGATCY 2 cut(s) 273, 874
BstXI CCANNNNNNTGG 1 cut(s) 773
BstYI RGATCY 2 cut(s) 273, 874
BsuRI GGCC 2 cut(s) 502, 904
BtgI CCRYGG 2 cut(s) 772, 848
BtrI CACGTC 1 cut(s) 1541
BtsCI GGATG 4 cut(s) 595, 844, 913, 1270
BtsI GCAGTG 1 cut(s) 1382
BtsIMutI CAGTG 1 cut(s) 1382
BveI ACCTGC 1 cut(s) 1271
Cac8I GCNNGC 3 cut(s) 442, 1015, 1257
CfoI GCGC 2 cut(s) 728, 966
Cfr10I RCCGGY 1 cut(s) 646
Cfr13I GGNCC 3 cut(s) 394, 500, 776
Csp6I GTAC 2 cut(s) 312, 534
CspAI ACCGGT 1 cut(s) 646
CviAII CATG 9 cut(s) 9, 773, 849, 967, 989, 1130, 1157, 1175, 1302
CviQI GTAC 2 cut(s) 312, 534
DdeI CTNAG 3 cut(s) 608, 882, 1461
DpnI GATC 7 cut(s) 275, 379, 420, 564, 681, 831, 876
DpnII GATC 7 cut(s) 273, 377, 418, 562, 679, 829, 874
DraIII CACNNNGTG 1 cut(s) 1203
DrdI GACNNNNNNGTC 1 cut(s) 1179
DseDI GACNNNNNNGTC 1 cut(s) 1179
Eco130I CCWWGG 3 cut(s) 638, 772, 848
Eco147I AGGCCT 1 cut(s) 904
Eco24I GRGCYC 2 cut(s) 446, 460
Eco47I GGWCC 2 cut(s) 394, 776
Eco57I CTGAAG 1 cut(s) 617
Eco91I GGTNACC 1 cut(s) 689
EcoNI CCTNNNNNAGG 1 cut(s) 1278
EcoO109I RGGNCCY 1 cut(s) 500
EcoO65I GGTNACC 1 cut(s) 689
EcoRI GAATTC 2 cut(s) 971, 1117
EcoT14I CCWWGG 3 cut(s) 638, 772, 848
EcoT38I GRGCYC 2 cut(s) 446, 460
ErhI CCWWGG 3 cut(s) 638, 772, 848
Esp3I CGTCTC 1 cut(s) 885
FaeI CATG 9 cut(s) 12, 776, 852, 970, 992, 1133, 1160, 1178, 1305
FalI AAGNNNNNCTT 4 cut(s) 739, 771, 1053, 1085
FaqI GGGAC 2 cut(s) 972, 1049
FatI CATG 9 cut(s) 8, 772, 848, 966, 988, 1129, 1156, 1174, 1301
FauI CCCGC 1 cut(s) 1018
FblI GTMKAC 2 cut(s) 27, 519
Fnu4HI GCNGC 6 cut(s) 210, 674, 719, 815, 1030, 1498
FokI GGATG 4 cut(s) 602, 831, 900, 1257
FriOI GRGCYC 2 cut(s) 446, 460
Fsp4HI GCNGC 6 cut(s) 210, 674, 719, 815, 1030, 1498
FspBI CTAG 1 cut(s) 1179
GlaI GCGC 2 cut(s) 727, 965
GluI GCNGC 6 cut(s) 210, 674, 719, 815, 1030, 1498
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 2 cut(s) 502, 904
HapII CCGG 1 cut(s) 647
HhaI GCGC 2 cut(s) 728, 966
Hin1II CATG 9 cut(s) 12, 776, 852, 970, 992, 1133, 1160, 1178, 1305
Hin6I GCGC 2 cut(s) 726, 964
HinP1I GCGC 2 cut(s) 726, 964
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 1 cut(s) 996
HinfI GANTC 7 cut(s) 4, 98, 521, 859, 1220, 1360, 1442
HpaI GTTAAC 1 cut(s) 699
HpaII CCGG 1 cut(s) 647
HphI GGTGA 4 cut(s) 52, 107, 385, 999
Hpy166II GTNNAC 6 cut(s) 28, 312, 520, 699, 733, 1259
Hpy188I TCNGA 5 cut(s) 83, 1225, 1335, 1462, 1524
Hpy188III TCNNGA 7 cut(s) 120, 259, 279, 491, 863, 1070, 1414
Hpy8I GTNNAC 6 cut(s) 28, 312, 520, 699, 733, 1259
HpyAV CCTTC 4 cut(s) 46, 167, 513, 592
HpyCH4III ACNGT 3 cut(s) 95, 451, 1396
HpyCH4IV ACGT 3 cut(s) 300, 314, 1540
HpyCH4V TGCA 6 cut(s) 209, 718, 980, 1195, 1259, 1375
HpyF10VI GCNNNNNNNGC 2 cut(s) 1286, 1311
HpyF3I CTNAG 3 cut(s) 608, 882, 1461
HpySE526I ACGT 3 cut(s) 300, 314, 1540
Hsp92II CATG 9 cut(s) 12, 776, 852, 970, 992, 1133, 1160, 1178, 1305
HspAI GCGC 2 cut(s) 726, 964
KspAI GTTAAC 1 cut(s) 699
Kzo9I GATC 7 cut(s) 273, 377, 418, 562, 679, 829, 874
LmnI GCTCC 3 cut(s) 571, 793, 1108
Lsp1109I GCAGC 5 cut(s) 221, 660, 730, 1016, 1509
LweI GCATC 2 cut(s) 805, 853
MaeI CTAG 1 cut(s) 1179
MaeII ACGT 3 cut(s) 300, 314, 1540
MaeIII GTNAC 2 cut(s) 689, 781
MalI GATC 7 cut(s) 275, 379, 420, 564, 681, 831, 876
MbiI CCGCTC 1 cut(s) 569
MboI GATC 7 cut(s) 273, 377, 418, 562, 679, 829, 874
MboII GAAGA 7 cut(s) 347, 480, 832, 1175, 1258, 1449, 1541
MflI RGATCY 2 cut(s) 273, 874
MhlI GDGCHC 4 cut(s) 446, 460, 1261, 1291
MlyI GAGTC 2 cut(s) 107, 515
MmeI TCCRAC 2 cut(s) 567, 1146
MroXI GAANNNNTTC 2 cut(s) 648, 824
MseI TTAA 7 cut(s) 180, 188, 353, 698, 1110, 1490, 1505
MslI CAYNNNNRTG 1 cut(s) 771
MspI CCGG 1 cut(s) 647
Mva1269I GAATGC 2 cut(s) 477, 859
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 1286, 1311
NcoI CCATGG 2 cut(s) 772, 848
NdeII GATC 7 cut(s) 273, 377, 418, 562, 679, 829, 874
NlaIII CATG 9 cut(s) 12, 776, 852, 970, 992, 1133, 1160, 1178, 1305
NlaIV GGNNCC 7 cut(s) 501, 645, 651, 876, 1095, 1288, 1384
NmeAIII GCCGAG 1 cut(s) 439
NspI RCATGY 2 cut(s) 1178, 1305
PaqCI CACCTGC 1 cut(s) 1271
PceI AGGCCT 1 cut(s) 904
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PctI GAATGC 2 cut(s) 477, 859
PdmI GAANNNNTTC 2 cut(s) 648, 824
PfeI GAWTC 5 cut(s) 4, 859, 1220, 1360, 1442
PinAI ACCGGT 1 cut(s) 646
PkrI GCNGC 6 cut(s) 211, 675, 720, 816, 1031, 1499
PleI GAGTC 2 cut(s) 106, 515
PpsI GAGTC 2 cut(s) 106, 515
PshBI ATTAAT 1 cut(s) 1110
PsiI TTATAA 1 cut(s) 1164
PspEI GGTNACC 1 cut(s) 689
PspFI CCCAGC 1 cut(s) 365
PspN4I GGNNCC 7 cut(s) 501, 645, 651, 876, 1095, 1288, 1384
PspPI GGNCC 3 cut(s) 394, 500, 776
PstI CTGCAG 1 cut(s) 1377
PsuI RGATCY 2 cut(s) 273, 874
RsaI GTAC 2 cut(s) 313, 535
RsaNI GTAC 2 cut(s) 312, 534
RseI CAYNNNNRTG 1 cut(s) 771
SaqAI TTAA 7 cut(s) 180, 188, 353, 698, 1110, 1490, 1505
SatI GCNGC 6 cut(s) 210, 674, 719, 815, 1030, 1498
Sau3AI GATC 7 cut(s) 273, 377, 418, 562, 679, 829, 874
Sau96I GGNCC 3 cut(s) 394, 500, 776
SchI GAGTC 2 cut(s) 107, 515
SduI GDGCHC 4 cut(s) 446, 460, 1261, 1291
SfaNI GCATC 2 cut(s) 805, 853
SfcI CTRYAG 4 cut(s) 213, 318, 1275, 1373
SinI GGWCC 2 cut(s) 394, 776
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 1343
SmoI CTYRAG 1 cut(s) 1343
SseBI AGGCCT 1 cut(s) 904
SsiI CCGC 4 cut(s) 569, 814, 1011, 1236
SspMI CTAG 1 cut(s) 1179
StuI AGGCCT 1 cut(s) 904
StyI CCWWGG 3 cut(s) 638, 772, 848
TaaI ACNGT 3 cut(s) 95, 451, 1396
TaiI ACGT 3 cut(s) 303, 317, 1543
TaqI TCGA 5 cut(s) 101, 244, 403, 565, 828
TauI GCSGC 1 cut(s) 817
TfiI GAWTC 5 cut(s) 4, 859, 1220, 1360, 1442
Tru1I TTAA 7 cut(s) 180, 188, 353, 698, 1110, 1490, 1505
Tru9I TTAA 7 cut(s) 180, 188, 353, 698, 1110, 1490, 1505
TscAI CASTG 1 cut(s) 1382
TseI GCWGC 5 cut(s) 209, 673, 718, 1029, 1497
TspDTI ATGAA 8 cut(s) 5, 105, 311, 908, 1005, 1285, 1367, 1542
TspGWI ACGGA 5 cut(s) 671, 806, 867, 913, 1179
TspRI CASTG 1 cut(s) 1382
VneI GTGCAC 1 cut(s) 1257
VpaK11BI GGWCC 2 cut(s) 394, 776
VspI ATTAAT 1 cut(s) 1110
XagI CCTNNNNNAGG 1 cut(s) 1278
XapI RAATTY 6 cut(s) 246, 358, 550, 950, 971, 1117
XceI RCATGY 2 cut(s) 1178, 1305
XmiI GTMKAC 2 cut(s) 27, 519
XmnI GAANNNNTTC 2 cut(s) 648, 824
XspI CTAG 1 cut(s) 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.