Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005739

Physical Location & Seq

Genomic Coordinates Forward (+)

26319597 .. 26326911

Gene Structure 7315 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1121900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3129 bp

ATGTCAGATGACCGTAACACGAAACGTACGCAAGTGGACAGTTGCGTTGCGTCCACCAAACACCAGGAAGTCTCGAGGAATGCAATCTCTCCCGTGGAAGTTGGGCCTTTAAATATAAAAAGGCGATTCAGACTAGAGGGACAAATCGTTGAAAACGACGGTAAGAGATTTCTGGGGCATTTCTCTCTCCCCCTCCCACAGATCATTACCCATTGCCTTTACCCCCACCAACTACCTTCAACTGCAATCCCTCCACGCTTCCTCCGCCGTCCCGCGGCTGTTGCTGCTTTTTCACATTCTCTCTCCGCGGCGATCTGGTTGTGTGAATCATCAGCCATGACAATTCCGGATTCGGATTTCATGATGGAAAACGGAGTTAGTTGCTTACCCAACACGCCGGAGGAGGAGAAGCGGATCATCGATGAATTGGCTCGAAAATCGGAGGCCAATGTCAAAGAAGGCAACTTGTTCTATGTTGTTTCCTATAGGTGGCACTCAAGCTGGAAGAGGTATGTTGAGCAAGGCACTGGAAAACATCTATATGACGAGCAGGATTTTGTGTCTCAAAAGTTGGATCTGCTCTCATCTAAAAATATAGCTAGACCTGGACCTATTGATAATTCTGATATAGTTGTAAATGAATCTGAGGGCAGCGATTTACAACTTCATAGTACACTGCTGGAAGAGCGTGACTATGTCTTAGTTTCTCAAGAAGTTTGGGAAAAGCTTTTCGACTGGTACAAAGGAGGTCCTGCATTACCAAGAAAGATGATTTCTCAGGGTGACCTTATCAAGAATCTGATGGTGGAGGTCTACCCGCTCTGTCTTAAGATTATTGATTCAAGAGACAGCAGCCAGACAGTTGTAAGATTAAGCAAAAAGGCTTCTGTACAGGAACTTTACGAGAAGGTGTACAAAATTAGAGGAATAGAGCAACAAAAGGCTCATATTTGGGACTACTTCAACATGGTGAAGAATTCATTATTGAGTGCTTCAAACCAAACCCTAGAGCAGCTGAACTTGCAGATGGATCAAGAAATTCTACTTGAGGTGCAAGTTAATGGGAACTTTTCTTCTCAATTCTCCATGGATTCAACAGGAAATGAGTTAGCATTGGTACCCCTAGAACCATCAAGGTCATCTATGACAATTGCAGGAGGGTCTTCCTTGTCAAATGGTCATTCAATGGATTATAGTTATAATAATTTGCACCAGGGAAGTGCCTTAAGCAGCTCAACATCAACATTTACAGATGATAAAAGTTATGTTTATAATCCAATGAAAAAATTAGATAGGGGAGGTTTGGCTGGATTGCAAAATCTTGGAAACACATGTTTTATGAATAGTTCACTTCAGTGCTTAGTTCATACACCTCCACTTGTTGAGTATTTCTTGCAAGATTACTCTGATGAGATCAACACTGACAATCCTTTAGGGATGCATGGTGAGCTTGCTCTTGCATTCGGTGAGTTGTTGAGAAAATTGTGGTCATCAGGACGAACAACAATTGCACCACGTGCTTTCAAGGGAAAACTTTCTCGATTTGCTCCCCAGTTTAGTGGCTATAATCAACATGATTCTCAAGAACTTCTTGCTTTCTTACTTGATGGACTACATGAAGATTTGAATCGTGTCAAGAATAAGCCGTACATTGAAACAAAAGATTCAGATGGTCGTCCAGATGAGGAAGTTGCAATTGAATGTTGGAAAAATCACAGGGCCCGAAATGATTCATTGATTGTGGATGTGTGCCAAGGTCAATATAAATCAACATTGGTATGCCCAGTTTGTAGCAAGGTTTCAATTACTTTTGATCCGTTTATGTACCTATCATTGCCACTACCTTCAACTGTCACCCGGTCAATGACGGTAACTGTGCTTTATGGAGATGGGCGTGGGCTTCCACTTCCGTACACTGTAACCTTGATAAAAGATCGGTGCTGTAAGGATCTTATTGAGGCATTGGCTAACGTATGCTGCTTGAACAGTGATGAAACCCTTCTGCTTGCTGAGGTATATGAACATCGAATTTATCGATATTTGGACAACCTTTCTGAACCATTGTCTTCAATTAAGAATGATGATCGGATTGTGGCCTATAGATATTCTAAAGAAGAAGTAGCTTCCAGAACCAGACTTGAAATTATTCATCGACGGCAGGAAAAATGTGCATTAGATCCTCTGAAGGGTCAGAGAAAGCTTTTTGGAACACCTTTGGTTGCCTATCTAGGAGAAGACCGGATAACTGGTGTTGATGTTGGTAGAGCTGTTTCTAAAATTCTGTCGCCCTTGAAGAGAGTGGTTAAGCTTCATAGCACGAAACAAAATGGATTAGTTTCAGAAGCCATTCATGAACCATCAAACAGCCACAATTTGAGGCCCACGGAGAACATAGAGCTAGAGGAATCATCAAGTGGCGAGTTATCCTTCCAGCTATTTTTAGCTGATGAGAGGGGTATGACCTGCAAACCACTTGAAAAGTCTTCATCCATAAGTCCCGGTAAAATCGTAAAGATATTTGCGGACTGGACTGACCAAGAAGACGAAGTATATGATGCTAGCTATCTCGGGGATCTACCTGAGGTACACAAGAATGGGTTCACAGCAAAGAAAACTCGGCAAGAAGCTATTTCCTTATTCACATGCTTGGAGGCATTTTTGAAGGAAGAACCTCTAGGTCCCGATGATATGTGGTACTGCCCTAAGTGCAAGGAACATAGACAAGCTACAAAGAAACTGGATTTGTGGATGTTGCCGGAGGTTCTTGTTTTTCACCTGAAACGGTTCTCATATAGCAGATACTCAAAGAATAAACTTGATACCCTGGTGACTTATCCAATTCTTAATCTTGATTTGAGTCAATATGTGAAAAGCAAGGATGGAAGGCCTCATCTCTATGAGCTATACGCCATTAGCAACCATTACGGCGGTCTAGGTGGTGGGCACTACACTGCATATGCGAAGTTGATTGATGAGAACAGATGGTACCATTTTGACGACAGTCACGTATCTCCAGTGGATGAAACTGATATCAAGACTTCGGCCGCCTATGTGCTGTTTTATCGGAGAGTTAAAAGTGGACAAAATACCGGAGAGGCAGAGTCATCAGGGACTCGTATGGAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

DUSP UCH

Protein Analysis

1043

Amino Acids

117.96

Weight (kDa)

6.03

Isoelectric Point (pI)

45.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUSP	PF06337	155 - 258	2.6e-22	DUSP domain
UCH	PF00443	436 - 1021	7.3e-77	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	436 - 615	1.7e-06	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005739)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32850 AT4G10570 AT4G10590 AT4G10590 AT4G10590 AT4G10590
fragaria_vesca	FvH4_5g10610 FvH4_5g10610 FvH4_5g10610
malus_domestica	MD06G1121900.v1.1 MD14G1138300.v1.1
prunus_persica	Prupe.5G132600_v2.0.a1 Prupe.5G132600_v2.0.a1 Prupe.5G132600_v2.0.a1
pyrus_communis	pycom06g11300 pycom14g11340
rosa_chinensis	RchiOBHm_Chr7g0187531
rosa_laevigata	RLG00000004773
rosa_multiflora	Rmu_sc0008640.1_g000004 Rmu_sc0014966.1_g000001 Rmu_sc0023622.1_g000001 Rmu_ssc0000110.1_g000024
rosa_roxburghii	Rroxscaffold_3G00267350
rosa_rugosa	Rorug06G0482500
rosa_samantha	Rh7AG087100 Rh7BG088500 Rh7CG087800 Rh7DG089400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1200, 1272
Acc36I	ACCTGC	1 cut(s)	2471
Acc65I	GGTACC	2 cut(s)	1117, 2985
AccB1I	GGYRCC	2 cut(s)	1117, 2985
AccBSI	CCGCTC	1 cut(s)	820
AccI	GTMKAC	1 cut(s)	813
AccII	CGCG	2 cut(s)	275, 308
AccIII	TCCGGA	1 cut(s)	346
AclWI	GGATC	7 cut(s)	422, 582, 1038, 1808, 1956, 2171, 2580
AcoI	YGGCCR	1 cut(s)	3042
AcsI	RAATTY	4 cut(s)	976, 1038, 2028, 2277
AcuI	CTGAAG	2 cut(s)	1337, 2204
AcvI	CACGTG	1 cut(s)	1517
AdeI	CACNNNGTG	1 cut(s)	1517
AfiI	CCNNNNNNNGG	3 cut(s)	274, 489, 2186
AflII	CTTAAG	2 cut(s)	827, 1225
AflIII	ACRYGT	1 cut(s)	1331
AjnI	CCWGG	4 cut(s)	63, 604, 1212, 2823
AleI	CACNNNNGTG	1 cut(s)	1354
Alw26I	GTCTC	3 cut(s)	76, 567, 840
AlwI	GGATC	7 cut(s)	422, 582, 1038, 1808, 1956, 2171, 2580
Ama87I	CYCGRG	2 cut(s)	73, 2567
Aor13HI	TCCGGA	1 cut(s)	346
AoxI	GGCC	7 cut(s)	104, 444, 1719, 2094, 2378, 2885, 3042
ApaI	GGGCCC	1 cut(s)	1723
ApeKI	GCWGC	6 cut(s)	284, 651, 852, 1012, 1230, 1977
ApoI	RAATTY	4 cut(s)	976, 1038, 2028, 2277
Asp700I	GAANNNNTTC	7 cut(s)	1534, 1729, 1998, 2145, 2346, 2597, 2783
Asp718I	GGTACC	2 cut(s)	1117, 2985
AspS9I	GGNCC	7 cut(s)	104, 608, 749, 1719, 1720, 2379, 2678
AsuC2I	CCSGG	2 cut(s)	1858, 2499
AsuHPI	GGTGA	7 cut(s)	794, 982, 1457, 1478, 1846, 2765, 2839
AsuNHI	GCTAGC	1 cut(s)	2558
AvaI	CYCGRG	2 cut(s)	73, 2567
AvaII	GGWCC	3 cut(s)	608, 749, 2678
AxyI	CCTNAGG	1 cut(s)	2580
BaeGI	GKGCMC	2 cut(s)	1723, 2946
BaeI	ACNNNNGTAYC	2 cut(s)	2811, 2844
BanI	GGYRCC	2 cut(s)	1117, 2985
BanII	GRGCYC	1 cut(s)	1723
BbrPI	CACGTG	1 cut(s)	1517
BbsI	GAAGAC	5 cut(s)	1155, 2058, 2241, 2475, 2547
BbvCI	CCTCAGC	1 cut(s)	2010
BbvI	GCAGC	6 cut(s)	271, 663, 864, 1024, 1242, 1964
BceAI	ACGGC	4 cut(s)	252, 1630, 2171, 2941
BciT130I	CCWGG	4 cut(s)	65, 606, 1214, 2825
BcnI	CCSGG	2 cut(s)	1858, 2499
BcoDI	GTCTC	3 cut(s)	76, 567, 840
BfaI	CTAG	9 cut(s)	134, 600, 1007, 1124, 2228, 2399, 2559, 2675, 2933
BfmI	CTRYAG	2 cut(s)	484, 2098
BfrI	CTTAAG	2 cut(s)	827, 1225
BfuAI	ACCTGC	1 cut(s)	2471
BisI	GCNGC	9 cut(s)	276, 285, 309, 652, 853, 1013, 1231, 1978, 3045
BlsI	GCNGC	9 cut(s)	277, 286, 310, 653, 854, 1014, 1232, 1979, 3046
Bme1390I	CCNGG	6 cut(s)	65, 606, 1214, 1858, 2499, 2825
Bme18I	GGWCC	3 cut(s)	608, 749, 2678
BmeT110I	CYCGRG	2 cut(s)	73, 2567
BmgT120I	GGNCC	7 cut(s)	104, 608, 749, 1719, 1720, 2379, 2678
BmiI	GGNNCC	4 cut(s)	1119, 1721, 2680, 2987
BmrFI	CCNGG	6 cut(s)	65, 606, 1214, 1858, 2499, 2825
BmrI	ACTGGG	2 cut(s)	1546, 1778
BmsI	GCATC	2 cut(s)	1428, 2545
BmtI	GCTAGC	1 cut(s)	2562
BmuI	ACTGGG	2 cut(s)	1546, 1778
BoxI	GACNNNNGTC	1 cut(s)	3000
BpiI	GAAGAC	5 cut(s)	1155, 2058, 2241, 2475, 2547
BpmI	CTGGAG	1 cut(s)	2997
Bpu10I	CCTNAGC	1 cut(s)	2010
BpuEI	CTTGAG	4 cut(s)	481, 693, 1067, 1566
BpuMI	CCSGG	2 cut(s)	1858, 2499
Bsa29I	ATCGAT	2 cut(s)	420, 2035
BsaAI	YACGTR	2 cut(s)	1517, 3007
BsaBI	GATNNNNATC	1 cut(s)	1626
BsaJI	CCNNGG	8 cut(s)	93, 273, 306, 1086, 1213, 1753, 2382, 2823
BsaWI	WCCGGW	3 cut(s)	346, 2238, 3089
BsaXI	ACNNNNNCTCC	2 cut(s)	246, 276
Bsc4I	CCNNNNNNNGG	3 cut(s)	274, 489, 2186
Bse1I	ACTGG	8 cut(s)	532, 740, 1552, 1784, 2251, 2531, 2742, 3014
Bse21I	CCTNAGG	1 cut(s)	2580
Bse3DI	GCAATG	2 cut(s)	211, 1832
Bse8I	GATNNNNATC	1 cut(s)	1626
BseAI	TCCGGA	1 cut(s)	346
BseBI	CCWGG	4 cut(s)	65, 606, 1214, 2825
BseCI	ATCGAT	2 cut(s)	420, 2035
BseDI	CCNNGG	8 cut(s)	93, 273, 306, 1086, 1213, 1753, 2382, 2823
BseGI	GGATG	6 cut(s)	1443, 1750, 2486, 2754, 2884, 3025
BseJI	GATNNNNATC	1 cut(s)	1626
BseLI	CCNNNNNNNGG	3 cut(s)	274, 489, 2186
BseMI	GCAATG	2 cut(s)	211, 1832
BseMII	CTCAG	4 cut(s)	636, 791, 2001, 2571
BseNI	ACTGG	8 cut(s)	532, 740, 1552, 1784, 2251, 2531, 2742, 3014
BseRI	GAGGAG	2 cut(s)	416, 419
BseSI	GKGCMC	2 cut(s)	1723, 2946
BseX3I	CGGCCG	1 cut(s)	3042
BseXI	GCAGC	6 cut(s)	271, 663, 864, 1024, 1242, 1964
Bsh1236I	CGCG	2 cut(s)	275, 308
Bsh1285I	CGRYCG	1 cut(s)	3045
BshFI	GGCC	7 cut(s)	106, 446, 1721, 2096, 2380, 2887, 3044
BshNI	GGYRCC	2 cut(s)	1117, 2985
BshVI	ATCGAT	2 cut(s)	420, 2035
BsiEI	CGRYCG	1 cut(s)	3045
BsiHKCI	CYCGRG	2 cut(s)	73, 2567
BsiSI	CCGG	7 cut(s)	347, 398, 1858, 2239, 2499, 2756, 3090
BsiWI	CGTACG	1 cut(s)	26
BslFI	GGGAC	6 cut(s)	153, 255, 968, 2481, 2664, 3124
BslI	CCNNNNNNNGG	3 cut(s)	274, 489, 2186
BsmAI	GTCTC	3 cut(s)	76, 567, 840
BsmFI	GGGAC	6 cut(s)	153, 255, 968, 2481, 2664, 3124
BsmI	GAATGC	2 cut(s)	85, 1460
BsnI	GGCC	7 cut(s)	106, 446, 1721, 2096, 2380, 2887, 3044
BsoBI	CYCGRG	2 cut(s)	73, 2567
Bsp120I	GGGCCC	1 cut(s)	1719
Bsp1286I	GDGCHC	2 cut(s)	1723, 2946
Bsp13I	TCCGGA	1 cut(s)	346
Bsp1407I	TGTACA	2 cut(s)	889, 912
Bsp19I	CCATGG	1 cut(s)	1086
BspANI	GGCC	7 cut(s)	106, 446, 1721, 2096, 2380, 2887, 3044
BspCNI	CTCAG	4 cut(s)	637, 790, 2002, 2572
BspDI	ATCGAT	2 cut(s)	420, 2035
BspEI	TCCGGA	1 cut(s)	346
BspFNI	CGCG	2 cut(s)	275, 308
BspHI	TCATGA	2 cut(s)	360, 2350
BspLI	GGNNCC	4 cut(s)	1119, 1721, 2680, 2987
BspMI	ACCTGC	1 cut(s)	2471
BspOI	GCTAGC	1 cut(s)	2562
BspPI	GGATC	7 cut(s)	422, 582, 1038, 1808, 1956, 2171, 2580
BspQI	GCTCTTC	1 cut(s)	678
BspT107I	GGYRCC	2 cut(s)	1117, 2985
BspTI	CTTAAG	2 cut(s)	827, 1225
BsrBI	CCGCTC	1 cut(s)	820
BsrDI	GCAATG	2 cut(s)	211, 1832
BsrGI	TGTACA	2 cut(s)	889, 912
BsrI	ACTGG	8 cut(s)	532, 740, 1552, 1784, 2251, 2531, 2742, 3014
BssECI	CCNNGG	8 cut(s)	93, 273, 306, 1086, 1213, 1753, 2382, 2823
BssT1I	CCWWGG	2 cut(s)	1086, 1753
Bst2UI	CCWGG	4 cut(s)	65, 606, 1214, 2825
Bst6I	CTCTTC	3 cut(s)	500, 678, 2288
BstAFI	CTTAAG	2 cut(s)	827, 1225
BstAPI	GCANNNNNTGC	1 cut(s)	1517
BstAUI	TGTACA	2 cut(s)	889, 912
BstBAI	YACGTR	2 cut(s)	1517, 3007
BstC8I	GCNNGC	3 cut(s)	1452, 2007, 2560
BstDEI	CTNAG	7 cut(s)	645, 700, 777, 1360, 2010, 2580, 2703
BstDSI	CCRYGG	5 cut(s)	93, 273, 306, 1086, 2382
BstEII	GGTNACC	1 cut(s)	782
BstF5I	GGATG	6 cut(s)	1443, 1750, 2486, 2754, 2884, 3025
BstFNI	CGCG	2 cut(s)	275, 308
BstMAI	GTCTC	3 cut(s)	76, 567, 840
BstMCI	CGRYCG	1 cut(s)	3045
BstMWI	GCNNNNNNNGC	8 cut(s)	264, 281, 284, 685, 1021, 1447, 1517, 2706
BstNI	CCWGG	4 cut(s)	65, 606, 1214, 2825
BstNSI	RCATGY	2 cut(s)	1335, 2646
BstPAI	GACNNNNGTC	1 cut(s)	3000
BstPI	GGTNACC	1 cut(s)	782
BstSCI	CCNGG	6 cut(s)	63, 604, 1212, 1856, 2497, 2823
BstSFI	CTRYAG	2 cut(s)	484, 2098
BstSLI	GKGCMC	2 cut(s)	1723, 2946
BstUI	CGCG	2 cut(s)	275, 308
BstV1I	GCAGC	6 cut(s)	271, 663, 864, 1024, 1242, 1964
BstV2I	GAAGAC	5 cut(s)	1155, 2058, 2241, 2475, 2547
BstX2I	RGATCY	4 cut(s)	574, 1948, 2176, 2572
BstXI	CCANNNNNNTGG	1 cut(s)	1559
BstYI	RGATCY	4 cut(s)	574, 1948, 2176, 2572
BstZI	CGGCCG	1 cut(s)	3042
Bsu15I	ATCGAT	2 cut(s)	420, 2035
Bsu36I	CCTNAGG	1 cut(s)	2580
BsuRI	GGCC	7 cut(s)	106, 446, 1721, 2096, 2380, 2887, 3044
BsuTUI	ATCGAT	2 cut(s)	420, 2035
BtgI	CCRYGG	5 cut(s)	93, 273, 306, 1086, 2382
BtsCI	GGATG	6 cut(s)	1443, 1750, 2486, 2754, 2884, 3025
BtsI	GCAGTG	2 cut(s)	674, 2949
BtsIMutI	CAGTG	8 cut(s)	525, 674, 1361, 1419, 1914, 1993, 2949, 3021
BveI	ACCTGC	1 cut(s)	2471
Cac8I	GCNNGC	3 cut(s)	1452, 2007, 2560
CciI	TCATGA	2 cut(s)	360, 2350
Cfr13I	GGNCC	7 cut(s)	104, 608, 749, 1719, 1720, 2379, 2678
Cfr42I	CCGCGG	2 cut(s)	276, 309
ClaI	ATCGAT	2 cut(s)	420, 2035
CseI	GACGC	1 cut(s)	39
DdeI	CTNAG	7 cut(s)	645, 700, 777, 1360, 2010, 2580, 2703
DraI	TTTAAA	1 cut(s)	111
DraIII	CACNNNGTG	1 cut(s)	1517
EaeI	YGGCCR	1 cut(s)	3042
EagI	CGGCCG	1 cut(s)	3042
Eam1104I	CTCTTC	3 cut(s)	500, 678, 2288
EarI	CTCTTC	3 cut(s)	500, 678, 2288
EciI	GGCGGA	1 cut(s)	254
EclXI	CGGCCG	1 cut(s)	3042
Eco130I	CCWWGG	2 cut(s)	1086, 1753
Eco147I	AGGCCT	1 cut(s)	2887
Eco24I	GRGCYC	1 cut(s)	1723
Eco32I	GATATC	1 cut(s)	3031
Eco47I	GGWCC	3 cut(s)	608, 749, 2678
Eco52I	CGGCCG	1 cut(s)	3042
Eco57I	CTGAAG	2 cut(s)	1337, 2204
Eco72I	CACGTG	1 cut(s)	1517
Eco81I	CCTNAGG	1 cut(s)	2580
Eco88I	CYCGRG	2 cut(s)	73, 2567
Eco91I	GGTNACC	1 cut(s)	782
EcoO109I	RGGNCCY	3 cut(s)	749, 1719, 2678
EcoO65I	GGTNACC	1 cut(s)	782
EcoRI	GAATTC	1 cut(s)	976
EcoRII	CCWGG	4 cut(s)	63, 604, 1212, 2823
EcoRV	GATATC	1 cut(s)	3031
EcoT14I	CCWWGG	2 cut(s)	1086, 1753
EcoT22I	ATGCAT	1 cut(s)	1443
EcoT38I	GRGCYC	1 cut(s)	1723
ErhI	CCWWGG	2 cut(s)	1086, 1753
FalI	AAGNNNNNCTT	2 cut(s)	1575, 1607
FaqI	GGGAC	6 cut(s)	153, 255, 968, 2481, 2664, 3124
FauI	CCCGC	2 cut(s)	280, 825
FauNDI	CATATG	1 cut(s)	2956
FblI	GTMKAC	1 cut(s)	813
Fnu4HI	GCNGC	9 cut(s)	276, 285, 309, 652, 853, 1013, 1231, 1978, 3045
FokI	GGATG	6 cut(s)	1450, 1757, 2473, 2761, 2891, 3032
FriOI	GRGCYC	1 cut(s)	1723
Fsp4HI	GCNGC	9 cut(s)	276, 285, 309, 652, 853, 1013, 1231, 1978, 3045
FspBI	CTAG	9 cut(s)	134, 600, 1007, 1124, 2228, 2399, 2559, 2675, 2933
GluI	GCNGC	9 cut(s)	276, 285, 309, 652, 853, 1013, 1231, 1978, 3045
GsuI	CTGGAG	1 cut(s)	2997
HaeIII	GGCC	7 cut(s)	106, 446, 1721, 2096, 2380, 2887, 3044
HapII	CCGG	7 cut(s)	347, 398, 1858, 2239, 2499, 2756, 3090
HgaI	GACGC	1 cut(s)	39
HindIII	AAGCTT	3 cut(s)	725, 2198, 2306
HpaII	CCGG	7 cut(s)	347, 398, 1858, 2239, 2499, 2756, 3090
HphI	GGTGA	7 cut(s)	794, 982, 1457, 1478, 1846, 2765, 2839
Hpy99I	CGWCG	2 cut(s)	161, 2157
HpyAV	CCTTC	9 cut(s)	246, 452, 901, 1854, 2009, 2179, 2437, 2656, 2877
HpyCH4IV	ACGT	4 cut(s)	25, 1516, 1971, 3006
HpyF10VI	GCNNNNNNNGC	8 cut(s)	264, 281, 284, 685, 1021, 1447, 1517, 2706
HpyF3I	CTNAG	7 cut(s)	645, 700, 777, 1360, 2010, 2580, 2703
HpySE526I	ACGT	4 cut(s)	25, 1516, 1971, 3006
Kpn2I	TCCGGA	1 cut(s)	346
KpnI	GGTACC	2 cut(s)	1121, 2989
KspI	CCGCGG	2 cut(s)	276, 309
LguI	GCTCTTC	1 cut(s)	678
LmnI	GCTCC	1 cut(s)	1552
Lsp1109I	GCAGC	6 cut(s)	271, 663, 864, 1024, 1242, 1964
LweI	GCATC	2 cut(s)	1428, 2545
MaeI	CTAG	9 cut(s)	134, 600, 1007, 1124, 2228, 2399, 2559, 2675, 2933
MaeII	ACGT	4 cut(s)	25, 1516, 1971, 3006
MaeIII	GTNAC	8 cut(s)	14, 689, 782, 1852, 1870, 1918, 2827, 3002
MbiI	CCGCTC	1 cut(s)	820
MfeI	CAATTG	3 cut(s)	1149, 1506, 1695
MflI	RGATCY	4 cut(s)	574, 1948, 2176, 2572
MhlI	GDGCHC	2 cut(s)	1723, 2946
MlyI	GAGTC	3 cut(s)	2866, 3106, 3110
MmeI	TCCRAC	2 cut(s)	552, 1685
Mph1103I	ATGCAT	1 cut(s)	1443
MroI	TCCGGA	1 cut(s)	346
MroXI	GAANNNNTTC	7 cut(s)	1534, 1729, 1998, 2145, 2346, 2597, 2783
MseI	TTAA	9 cut(s)	110, 828, 872, 1059, 1226, 2073, 2304, 2844, 3072
MslI	CAYNNNNRTG	5 cut(s)	540, 1354, 1777, 2592, 2895
MspA1I	CMGCKG	3 cut(s)	275, 308, 1015
MspCI	CTTAAG	2 cut(s)	827, 1225
MspI	CCGG	7 cut(s)	347, 398, 1858, 2239, 2499, 2756, 3090
MspR9I	CCNGG	6 cut(s)	65, 606, 1214, 1858, 2499, 2825
MunI	CAATTG	3 cut(s)	1149, 1506, 1695
Mva1269I	GAATGC	2 cut(s)	85, 1460
MvaI	CCWGG	4 cut(s)	65, 606, 1214, 2825
MvnI	CGCG	2 cut(s)	275, 308
MwoI	GCNNNNNNNGC	8 cut(s)	264, 281, 284, 685, 1021, 1447, 1517, 2706
NciI	CCSGG	2 cut(s)	1858, 2499
NcoI	CCATGG	1 cut(s)	1086
NdeI	CATATG	1 cut(s)	2956
NheI	GCTAGC	1 cut(s)	2558
NlaIV	GGNNCC	4 cut(s)	1119, 1721, 2680, 2987
NmeAIII	GCCGAG	1 cut(s)	2596
NmuCI	GTSAC	5 cut(s)	689, 782, 1852, 2827, 3002
NsiI	ATGCAT	1 cut(s)	1443
NspI	RCATGY	2 cut(s)	1335, 2646
OliI	CACNNNNGTG	1 cut(s)	1354
PaeR7I	CTCGAG	1 cut(s)	73
PagI	TCATGA	2 cut(s)	360, 2350
PceI	AGGCCT	1 cut(s)	2887
PciI	ACATGT	1 cut(s)	1331
PciSI	GCTCTTC	1 cut(s)	678
PcsI	WCGNNNNNNNCGW	3 cut(s)	153, 2032, 3003
PctI	GAATGC	2 cut(s)	85, 1460
PdmI	GAANNNNTTC	7 cut(s)	1534, 1729, 1998, 2145, 2346, 2597, 2783
Pfl23II	CGTACG	1 cut(s)	26
PflFI	GACNNNGTC	1 cut(s)	695
PkrI	GCNGC	9 cut(s)	277, 286, 310, 653, 854, 1014, 1232, 1979, 3046
PleI	GAGTC	3 cut(s)	2865, 3106, 3109
PmaCI	CACGTG	1 cut(s)	1517
PmlI	CACGTG	1 cut(s)	1517
PpsI	GAGTC	3 cut(s)	2865, 3106, 3109
Ppu21I	YACGTR	2 cut(s)	1517, 3007
PpuMI	RGGWCCY	2 cut(s)	749, 2678
PscI	ACATGT	1 cut(s)	1331
PshAI	GACNNNNGTC	1 cut(s)	3000
PsiI	TTATAA	2 cut(s)	1200, 1272
Psp5II	RGGWCCY	2 cut(s)	749, 2678
Psp6I	CCWGG	4 cut(s)	63, 604, 1212, 2823
PspCI	CACGTG	1 cut(s)	1517
PspEI	GGTNACC	1 cut(s)	782
PspGI	CCWGG	4 cut(s)	63, 604, 1212, 2823
PspLI	CGTACG	1 cut(s)	26
PspN4I	GGNNCC	4 cut(s)	1119, 1721, 2680, 2987
PspOMI	GGGCCC	1 cut(s)	1719
PspPI	GGNCC	7 cut(s)	104, 608, 749, 1719, 1720, 2379, 2678
PspPPI	RGGWCCY	2 cut(s)	749, 2678
PsrI	GAACNNNNNNTAC	2 cut(s)	2969, 3001
PsuI	RGATCY	4 cut(s)	574, 1948, 2176, 2572
PsyI	GACNNNGTC	1 cut(s)	695
PvuII	CAGCTG	1 cut(s)	1015
RseI	CAYNNNNRTG	5 cut(s)	540, 1354, 1777, 2592, 2895
SacII	CCGCGG	2 cut(s)	276, 309
SapI	GCTCTTC	1 cut(s)	678
SaqAI	TTAA	9 cut(s)	110, 828, 872, 1059, 1226, 2073, 2304, 2844, 3072
SatI	GCNGC	9 cut(s)	276, 285, 309, 652, 853, 1013, 1231, 1978, 3045
Sau96I	GGNCC	7 cut(s)	104, 608, 749, 1719, 1720, 2379, 2678
SchI	GAGTC	3 cut(s)	2866, 3106, 3110
ScrFI	CCNGG	6 cut(s)	65, 606, 1214, 1858, 2499, 2825
SduI	GDGCHC	2 cut(s)	1723, 2946
SfaNI	GCATC	2 cut(s)	1428, 2545
SfcI	CTRYAG	2 cut(s)	484, 2098
Sfr274I	CTCGAG	1 cut(s)	73
Sfr303I	CCGCGG	2 cut(s)	276, 309
SgrBI	CCGCGG	2 cut(s)	276, 309
SinI	GGWCC	3 cut(s)	608, 749, 2678
SlaI	CTCGAG	1 cut(s)	73
SmiMI	CAYNNNNRTG	5 cut(s)	540, 1354, 1777, 2592, 2895
SmlI	CTYRAG	7 cut(s)	73, 496, 708, 827, 1046, 1225, 1581
SmoI	CTYRAG	7 cut(s)	73, 496, 708, 827, 1046, 1225, 1581
SseBI	AGGCCT	1 cut(s)	2887
SspMI	CTAG	9 cut(s)	134, 600, 1007, 1124, 2228, 2399, 2559, 2675, 2933
StuI	AGGCCT	1 cut(s)	2887
StyD4I	CCNGG	6 cut(s)	63, 604, 1212, 1856, 2497, 2823
StyI	CCWWGG	2 cut(s)	1086, 1753
TaiI	ACGT	4 cut(s)	28, 1519, 1974, 3009
TaqI	TCGA	8 cut(s)	74, 420, 433, 732, 1540, 2026, 2035, 2152
TatI	WGTACW	3 cut(s)	671, 889, 912
TauI	GCSGC	3 cut(s)	278, 311, 3047
Tru1I	TTAA	9 cut(s)	110, 828, 872, 1059, 1226, 2073, 2304, 2844, 3072
Tru9I	TTAA	9 cut(s)	110, 828, 872, 1059, 1226, 2073, 2304, 2844, 3072
TscAI	CASTG	8 cut(s)	532, 681, 1361, 1426, 1921, 1993, 2956, 3021
TseFI	GTSAC	5 cut(s)	689, 782, 1852, 2827, 3002
TseI	GCWGC	6 cut(s)	284, 651, 852, 1012, 1230, 1977
Tsp45I	GTSAC	5 cut(s)	689, 782, 1852, 2827, 3002
TspGWI	ACGGA	4 cut(s)	387, 1806, 1899, 2399
TspRI	CASTG	8 cut(s)	532, 681, 1361, 1426, 1921, 1993, 2956, 3021
Tth111I	GACNNNGTC	1 cut(s)	695
Vha464I	CTTAAG	2 cut(s)	827, 1225
VpaK11BI	GGWCC	3 cut(s)	608, 749, 2678
XapI	RAATTY	4 cut(s)	976, 1038, 2028, 2277
XceI	RCATGY	2 cut(s)	1335, 2646
XhoI	CTCGAG	1 cut(s)	73
XmiI	GTMKAC	1 cut(s)	813
XmnI	GAANNNNTTC	7 cut(s)	1534, 1729, 1998, 2145, 2346, 2597, 2783
XspI	CTAG	9 cut(s)	134, 600, 1007, 1124, 2228, 2399, 2559, 2675, 2933
Zsp2I	ATGCAT	1 cut(s)	1443

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.